FvH4_6g24450

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000620
Physical Location & Seq
Reverse (-)
18544659 .. 18546564
1906 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24450.t1

Sequence Viewer

Length: 933 bp
ATGGTTTTCTTCTCATGCATCCCAATAAAGCCATATAGGTTTCTGCGGAAACGTTGTAGATCTTCGTCTATCAAGTCATCCACAAATGACTCCCGCAAGTTGTCTGCTAAGGATCCCAGTGAGTTCTCTTCTCGCAGTGGTGACATTCACAATCAAAATCTTAGCCAAAACCCAGCGACATATGAAGCGGTACAAGAGCTTCCCTTCGACATCATCACTGAGATTTTGAGTTGGCTGCCAGTAGAGTCTCTGCTCAGGTTCAAGTGTGTGTGCAAAAATTGGCGTTCCTTGCTTGGAGACTACAAGTTCATTGCAAAACATATGGCTCGAGCTAGATCTCATCAACTTTCCTGTCAACGTATAGTGGAACCTACTGCTAGTCCTACCAAACAGGAGGGCGATGGAGAATTTGGCCATAAAGTAGGGTTTAAAGTTCTAAGTATTGGAAAGGATGTTGAGTGGAGAACCCTGAAGCTGCCCAGGCAAAATGAAAGTCGGGTGATGATAAGAAGACGTATTGCTAATGCTAGAGCACTTCCTAATGAAGGGGTCGCTCATTTGGTGGACCTTATTGAAGTGGGAAAAGATTTCCAGCTAGAAGTTCTATCCTTTGATCTATGGAATCGTATAGCCGTTGCTGATATAGTAGGGGAAGCCCTTAACGTCTTGGTGTTACAAGACTTCAAGGAGCACAAATGGAGTAACATCACTGTTCCCTTAGAGTTTCTGAAGCATGACCCAGTTTTGAAAGATCAAATTAAACCTATCCTAGCTACTAGGTTTGGTAAACTCCAGTTCACCAATCCTGTGAGAAATGACATTCTTGAATATGATATGGAACGGGAGAAGATTACGGCGACGGGTACAAAGTCCACAGAGCAAAAAATATACTTGGTCAAGCCAAGCCTTGTTACTCTCAAGGGAATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

311

Amino Acids

35.55

Weight (kDa)

9.53

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 65 - 100 5.7e-08 F-box-like
F-box PF00646 66 - 103 1.7e-11 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 46, 94, 188
AclI AACGTT 1 cut(s) 52
AclWI GGATC 2 cut(s) 107, 120
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 1 cut(s) 407
AcuI CTGAAG 2 cut(s) 491, 749
AfaI GTAC 2 cut(s) 192, 865
AfiI CCNNNNNNNGG 1 cut(s) 545
AgsI TTSAA 5 cut(s) 262, 575, 685, 748, 827
AjnI CCWGG 1 cut(s) 479
AluBI AGCT 5 cut(s) 199, 332, 475, 595, 773
AluI AGCT 5 cut(s) 199, 332, 475, 595, 773
Alw21I GWGCWC 2 cut(s) 535, 693
Alw26I GTCTC 2 cut(s) 252, 291
AlwI GGATC 2 cut(s) 107, 120
Ama87I CYCGRG 1 cut(s) 327
AoxI GGCC 1 cut(s) 412
ApeKI GCWGC 2 cut(s) 235, 475
ApoI RAATTY 1 cut(s) 407
ArsI GACNNNNNNTTYG 2 cut(s) 478, 510
AspS9I GGNCC 1 cut(s) 565
AsuHPI GGTGA 3 cut(s) 152, 511, 790
AvaI CYCGRG 1 cut(s) 327
AvaII GGWCC 1 cut(s) 565
BalI TGGCCA 1 cut(s) 414
BamHI GGATCC 1 cut(s) 112
BarI GAAGNNNNNNTAC 2 cut(s) 183, 215
BbsI GAAGAC 1 cut(s) 517
Bbv12I GWGCWC 2 cut(s) 535, 693
BbvI GCAGC 2 cut(s) 222, 462
BccI CCATC 1 cut(s) 395
BceAI ACGGC 2 cut(s) 617, 870
BciT130I CCWGG 1 cut(s) 481
BcoDI GTCTC 2 cut(s) 252, 291
BfaI CTAG 6 cut(s) 333, 378, 528, 596, 770, 777
BglII AGATCT 2 cut(s) 59, 335
BisI GCNGC 2 cut(s) 236, 476
BlsI GCNGC 2 cut(s) 237, 477
Bme1390I CCNGG 1 cut(s) 481
Bme18I GGWCC 1 cut(s) 565
BmeT110I CYCGRG 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 565
BmiI GGNNCC 2 cut(s) 114, 369
BmrFI CCNGG 1 cut(s) 481
BmrI ACTGGG 2 cut(s) 111, 734
BmsI GCATC 1 cut(s) 27
BmuI ACTGGG 2 cut(s) 111, 734
BpiI GAAGAC 1 cut(s) 517
BpmI CTGGAG 1 cut(s) 776
Bpu10I CCTNAGC 2 cut(s) 108, 254
BpuEI CTTGAG 1 cut(s) 902
BsaJI CCNNGG 1 cut(s) 479
Bsc4I CCNNNNNNNGG 1 cut(s) 545
Bse1I ACTGG 4 cut(s) 117, 239, 740, 793
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 479
BseGI GGATG 3 cut(s) 18, 77, 457
BseLI CCNNNNNNNGG 1 cut(s) 545
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 2 cut(s) 210, 268
BseNI ACTGG 4 cut(s) 117, 239, 740, 793
BseXI GCAGC 2 cut(s) 222, 462
BseYI CCCAGC 1 cut(s) 172
BshFI GGCC 1 cut(s) 414
BsiHKAI GWGCWC 2 cut(s) 535, 693
BsiHKCI CYCGRG 1 cut(s) 327
BslI CCNNNNNNNGG 1 cut(s) 545
BsmAI GTCTC 2 cut(s) 252, 291
BsnI GGCC 1 cut(s) 414
BsoBI CYCGRG 1 cut(s) 327
Bsp1286I GDGCHC 2 cut(s) 535, 693
Bsp143I GATC 5 cut(s) 59, 112, 335, 613, 751
BspACI CCGC 3 cut(s) 46, 94, 188
BspANI GGCC 1 cut(s) 414
BspCNI CTCAG 2 cut(s) 211, 267
BspLI GGNNCC 2 cut(s) 114, 369
BspPI GGATC 2 cut(s) 107, 120
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 4 cut(s) 117, 239, 740, 793
BssECI CCNNGG 1 cut(s) 479
BssMI GATC 5 cut(s) 59, 112, 335, 613, 751
Bst2UI CCWGG 1 cut(s) 481
Bst4CI ACNGT 1 cut(s) 712
Bst6I CTCTTC 1 cut(s) 133
BstDEI CTNAG 6 cut(s) 108, 161, 219, 254, 437, 718
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 3 cut(s) 18, 77, 457
BstKTI GATC 5 cut(s) 62, 115, 338, 616, 754
BstMAI GTCTC 2 cut(s) 252, 291
BstMBI GATC 5 cut(s) 59, 112, 335, 613, 751
BstMWI GCNNNNNNNGC 2 cut(s) 289, 481
BstNI CCWGG 1 cut(s) 481
BstSCI CCNGG 1 cut(s) 479
BstV1I GCAGC 2 cut(s) 222, 462
BstV2I GAAGAC 1 cut(s) 517
BstX2I RGATCY 3 cut(s) 59, 112, 335
BstYI RGATCY 3 cut(s) 59, 112, 335
BsuRI GGCC 1 cut(s) 414
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 3 cut(s) 18, 77, 457
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 4 cut(s) 124, 142, 216, 708
Cfr13I GGNCC 1 cut(s) 565
Csp6I GTAC 2 cut(s) 191, 864
CviAII CATG 2 cut(s) 15, 734
CviQI GTAC 2 cut(s) 191, 864
DdeI CTNAG 6 cut(s) 108, 161, 219, 254, 437, 718
DpnI GATC 5 cut(s) 61, 114, 337, 615, 753
DpnII GATC 5 cut(s) 59, 112, 335, 613, 751
DraI TTTAAA 1 cut(s) 430
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 565
Eco57I CTGAAG 2 cut(s) 491, 749
Eco88I CYCGRG 1 cut(s) 327
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 479
EcoT22I ATGCAT 1 cut(s) 20
FaeI CATG 2 cut(s) 18, 737
FatI CATG 2 cut(s) 14, 733
FauI CCCGC 1 cut(s) 101
FauNDI CATATG 2 cut(s) 181, 321
Fnu4HI GCNGC 2 cut(s) 236, 476
FokI GGATG 3 cut(s) 5, 64, 464
Fsp4HI GCNGC 2 cut(s) 236, 476
FspBI CTAG 6 cut(s) 333, 378, 528, 596, 770, 777
GluI GCNGC 2 cut(s) 236, 476
GsaI CCCAGC 1 cut(s) 176
GsuI CTGGAG 1 cut(s) 776
HaeIII GGCC 1 cut(s) 414
Hin1II CATG 2 cut(s) 18, 737
HincII GTYRAC 1 cut(s) 356
HindII GTYRAC 1 cut(s) 356
HinfI GANTC 3 cut(s) 89, 245, 622
HphI GGTGA 3 cut(s) 152, 511, 790
Hpy166II GTNNAC 5 cut(s) 356, 565, 788, 798, 873
Hpy188I TCNGA 1 cut(s) 729
Hpy188III TCNNGA 1 cut(s) 824
Hpy8I GTNNAC 5 cut(s) 356, 565, 788, 798, 873
Hpy99I CGWCG 1 cut(s) 862
HpyAV CCTTC 2 cut(s) 214, 539
HpyCH4III ACNGT 1 cut(s) 712
HpyCH4IV ACGT 4 cut(s) 52, 358, 514, 663
HpyCH4V TGCA 3 cut(s) 18, 273, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 289, 481
HpyF3I CTNAG 6 cut(s) 108, 161, 219, 254, 437, 718
HpySE526I ACGT 4 cut(s) 52, 358, 514, 663
Hsp92II CATG 2 cut(s) 18, 737
Kzo9I GATC 5 cut(s) 59, 112, 335, 613, 751
LmnI GCTCC 1 cut(s) 688
Lsp1109I GCAGC 2 cut(s) 222, 462
LweI GCATC 1 cut(s) 27
MaeI CTAG 6 cut(s) 333, 378, 528, 596, 770, 777
MaeII ACGT 4 cut(s) 52, 358, 514, 663
MaeIII GTNAC 4 cut(s) 140, 672, 701, 910
MalI GATC 5 cut(s) 61, 114, 337, 615, 753
MboI GATC 5 cut(s) 59, 112, 335, 613, 751
MboII GAAGA 4 cut(s) 54, 120, 522, 859
MflI RGATCY 3 cut(s) 59, 112, 335
MhlI GDGCHC 2 cut(s) 535, 693
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 3 cut(s) 277, 407, 756
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 2 cut(s) 83, 254
MnlI CCTC 1 cut(s) 388
Mox20I TGGCCA 1 cut(s) 414
Mph1103I ATGCAT 1 cut(s) 20
MscI TGGCCA 1 cut(s) 414
MseI TTAA 3 cut(s) 429, 660, 759
Msp20I TGGCCA 1 cut(s) 414
MspR9I CCNGG 1 cut(s) 481
MvaI CCWGG 1 cut(s) 481
MwoI GCNNNNNNNGC 2 cut(s) 289, 481
NdeI CATATG 2 cut(s) 181, 321
NdeII GATC 5 cut(s) 59, 112, 335, 613, 751
NlaIII CATG 2 cut(s) 18, 737
NlaIV GGNNCC 2 cut(s) 114, 369
NmuCI GTSAC 1 cut(s) 140
NsiI ATGCAT 1 cut(s) 20
PaeR7I CTCGAG 1 cut(s) 327
PfeI GAWTC 1 cut(s) 622
PkrI GCNGC 2 cut(s) 237, 477
PleI GAGTC 2 cut(s) 83, 253
PpsI GAGTC 2 cut(s) 83, 253
Psp1406I AACGTT 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 479
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 1 cut(s) 479
PspN4I GGNNCC 2 cut(s) 114, 369
PspPI GGNCC 1 cut(s) 565
PspXI VCTCGAGB 1 cut(s) 327
PsuI RGATCY 3 cut(s) 59, 112, 335
RsaI GTAC 2 cut(s) 192, 865
RsaNI GTAC 2 cut(s) 191, 864
SaqAI TTAA 3 cut(s) 429, 660, 759
SatI GCNGC 2 cut(s) 236, 476
Sau3AI GATC 5 cut(s) 59, 112, 335, 613, 751
Sau96I GGNCC 1 cut(s) 565
SchI GAGTC 2 cut(s) 83, 254
ScrFI CCNGG 1 cut(s) 481
SduI GDGCHC 2 cut(s) 535, 693
SfaNI GCATC 1 cut(s) 27
Sfr274I CTCGAG 1 cut(s) 327
SinI GGWCC 1 cut(s) 565
SlaI CTCGAG 1 cut(s) 327
SmlI CTYRAG 2 cut(s) 327, 917
SmoI CTYRAG 2 cut(s) 327, 917
Sse9I AATT 3 cut(s) 277, 407, 756
SsiI CCGC 3 cut(s) 46, 94, 188
SspMI CTAG 6 cut(s) 333, 378, 528, 596, 770, 777
StyD4I CCNGG 1 cut(s) 479
TaaI ACNGT 1 cut(s) 712
TaiI ACGT 4 cut(s) 55, 361, 517, 666
TaqI TCGA 2 cut(s) 207, 328
TasI AATT 3 cut(s) 277, 407, 756
TfiI GAWTC 1 cut(s) 622
Tru1I TTAA 3 cut(s) 429, 660, 759
Tru9I TTAA 3 cut(s) 429, 660, 759
TscAI CASTG 4 cut(s) 124, 142, 223, 715
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 2 cut(s) 235, 475
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 4 cut(s) 198, 298, 504, 558
TspRI CASTG 4 cut(s) 124, 142, 223, 715
VpaK11BI GGWCC 1 cut(s) 565
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 1 cut(s) 407
XhoI CTCGAG 1 cut(s) 327
XspI CTAG 6 cut(s) 333, 378, 528, 596, 770, 777
Zsp2I ATGCAT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.