Rmu_sc0002454.1_g000005

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002454.1
OG0000620
Physical Location & Seq
Forward (+)
32264 .. 33301
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002454.1_g000005.1.cds

Sequence Viewer

Length: 1038 bp
atggtgcgttcgaacaaaagaaataaagggagtagtttggaagccataaaactaccaaaacttccttttgataccatcattgagattttgagctggttgccagtcgtgtctttgctaaggtgcaagtgtgtgtgcaagcaatggtgttcgttgattcaagatgacaagttcgttctaaaacatatggatcgaagccgtccaaccgatcagttttatcgtaccgctgatgaaaagttcaagtttatttcaacttatggaggcttgtgtttggagagaagtcttagtaattctagggtttttcgtatcagaaaccctgcagcccaacaagtgctatacttgcctgatggacctgagtatactaacaccatggattttgcttttaattcatctactcgcgagtgtaaagtggtatgttgttgttgggagaacaacgaagttcacagtactgaagcaagtttgaaaattataactcttggaaaagatgagcaatggagaactctgatcctcccaaaccaaagtttgatgaatgcgcgcggcgagttcgtgttaaaagtgcacttcagtggagtacgtaaggttgaaggggttgttcatttgacccaaataattagacttactgcaccagattattacttacaagttcaatctttggatgtttggagagaatctttcaccactactactctgcctcagggagttttcgtagacctgacaaaagttcggggttatacttggaataattgcacgtctgttgttgatatgacagaggaaaccctcagcattttagccttggaagacttcaaagaacacaagtggagtaaaaacaaaatcaaaattcccttgaaagttctgaaggattgtcatccacatttgagggaccgcactgttccggctgcattcgaattactatttggagcgagctatgattttaaatataataagagaaaagggatagttaccaaggccacacttagaccttctggggaaagattacataagccaagtctcatgacgcttagaggaatgaagtcggaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

345

Amino Acids

39.81

Weight (kDa)

9.32

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AccI GTMKAC 2 cut(s) 356, 705
AccII CGCG 3 cut(s) 396, 532, 534
AciI CCGC 3 cut(s) 222, 534, 880
AclWI GGATC 2 cut(s) 195, 496
AcsI RAATTY 1 cut(s) 834
AcuI CTGAAG 3 cut(s) 468, 544, 872
AfaI GTAC 3 cut(s) 220, 445, 570
AgsI TTSAA 8 cut(s) 158, 238, 249, 460, 581, 644, 802, 844
AjiI CACGTC 1 cut(s) 747
AjuI GAANNNNNNNTTGG 2 cut(s) 894, 926
AleI CACNNNNGTG 1 cut(s) 561
AluBI AGCT 2 cut(s) 93, 921
AluI AGCT 2 cut(s) 93, 921
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 1 cut(s) 1010
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 2 cut(s) 195, 496
AoxI GGCC 1 cut(s) 963
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 2 cut(s) 317, 893
ApoI RAATTY 1 cut(s) 834
AspLEI GCGC 2 cut(s) 532, 534
AspS9I GGNCC 2 cut(s) 347, 877
AsuHPI GGTGA 1 cut(s) 664
AsuII TTCGAA 2 cut(s) 11, 900
AvaII GGWCC 2 cut(s) 347, 877
AxyI CCTNAGG 1 cut(s) 690
BaeGI GKGCMC 1 cut(s) 558
BbsI GAAGAC 1 cut(s) 801
Bbv12I GWGCWC 1 cut(s) 558
BbvCI CCTCAGC 1 cut(s) 776
BbvI GCAGC 2 cut(s) 329, 880
BccI CCATC 2 cut(s) 83, 338
BceAI ACGGC 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 1010
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 315
BisI GCNGC 3 cut(s) 318, 535, 894
BlsI GCNGC 3 cut(s) 319, 536, 895
BmcAI AGTACT 1 cut(s) 445
Bme18I GGWCC 2 cut(s) 347, 877
BmgBI CACGTC 1 cut(s) 747
BmgT120I GGNCC 2 cut(s) 347, 877
BmiI GGNNCC 1 cut(s) 878
BpiI GAAGAC 1 cut(s) 801
Bpu10I CCTNAGC 2 cut(s) 116, 776
Bpu14I TTCGAA 2 cut(s) 11, 900
BsaAI YACGTR 1 cut(s) 572
BsaBI GATNNNNATC 1 cut(s) 861
BsaJI CCNNGG 3 cut(s) 366, 789, 960
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bse1I ACTGG 1 cut(s) 101
Bse21I CCTNAGG 1 cut(s) 690
Bse3DI GCAATG 2 cut(s) 146, 494
Bse8I GATNNNNATC 1 cut(s) 861
BseDI CCNNGG 3 cut(s) 366, 789, 960
BseGI GGATG 2 cut(s) 658, 862
BseJI GATNNNNATC 1 cut(s) 861
BseMI GCAATG 2 cut(s) 146, 494
BseMII CTCAG 3 cut(s) 342, 704, 790
BseNI ACTGG 1 cut(s) 101
BsePI GCGCGC 1 cut(s) 530
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 2 cut(s) 329, 880
BsgI GTGCAG 1 cut(s) 603
Bsh1236I CGCG 3 cut(s) 396, 532, 534
BshFI GGCC 1 cut(s) 965
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 1 cut(s) 890
BslFI GGGAC 1 cut(s) 890
BsmAI GTCTC 1 cut(s) 1010
BsmFI GGGAC 1 cut(s) 890
BsmI GAATGC 2 cut(s) 532, 896
BsnI GGCC 1 cut(s) 965
Bsp119I TTCGAA 2 cut(s) 11, 900
Bsp1286I GDGCHC 1 cut(s) 558
Bsp143I GATC 3 cut(s) 187, 205, 501
Bsp19I CCATGG 1 cut(s) 366
Bsp68I TCGCGA 1 cut(s) 396
BspACI CCGC 3 cut(s) 222, 534, 880
BspANI GGCC 1 cut(s) 965
BspCNI CTCAG 3 cut(s) 343, 703, 789
BspFNI CGCG 3 cut(s) 396, 532, 534
BspHI TCATGA 1 cut(s) 1008
BspLI GGNNCC 1 cut(s) 878
BspMAI CTGCAG 1 cut(s) 319
BspPI GGATC 2 cut(s) 195, 496
BspT104I TTCGAA 2 cut(s) 11, 900
BsrDI GCAATG 2 cut(s) 146, 494
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 3 cut(s) 366, 789, 960
BssHII GCGCGC 1 cut(s) 530
BssMI GATC 3 cut(s) 187, 205, 501
BssNAI GTATAC 1 cut(s) 357
BssT1I CCWWGG 3 cut(s) 366, 789, 960
Bst1107I GTATAC 1 cut(s) 357
Bst4CI ACNGT 2 cut(s) 443, 886
BstBAI YACGTR 1 cut(s) 572
BstBI TTCGAA 2 cut(s) 11, 900
BstC8I GCNNGC 3 cut(s) 137, 532, 919
BstDEI CTNAG 7 cut(s) 116, 281, 351, 690, 776, 971, 1016
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 2 cut(s) 658, 862
BstFNI CGCG 3 cut(s) 396, 532, 534
BstHHI GCGC 2 cut(s) 532, 534
BstKTI GATC 3 cut(s) 190, 208, 504
BstMAI GTCTC 1 cut(s) 1010
BstMBI GATC 3 cut(s) 187, 205, 501
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 1 cut(s) 558
BstSNI TACGTA 1 cut(s) 572
BstUI CGCG 3 cut(s) 396, 532, 534
BstV1I GCAGC 2 cut(s) 329, 880
BstV2I GAAGAC 1 cut(s) 801
BstZ17I GTATAC 1 cut(s) 357
Bsu36I CCTNAGG 1 cut(s) 690
BsuRI GGCC 1 cut(s) 965
BtgI CCRYGG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 747
BtsCI GGATG 2 cut(s) 658, 862
BtsIMutI CAGTG 2 cut(s) 568, 882
BtuMI TCGCGA 1 cut(s) 396
Cac8I GCNNGC 3 cut(s) 137, 532, 919
CciI TCATGA 1 cut(s) 1008
CfoI GCGC 2 cut(s) 532, 534
Cfr13I GGNCC 2 cut(s) 347, 877
CseI GACGC 1 cut(s) 1021
Csp6I GTAC 3 cut(s) 219, 444, 569
CviAII CATG 2 cut(s) 367, 1009
CviQI GTAC 3 cut(s) 219, 444, 569
DdeI CTNAG 7 cut(s) 116, 281, 351, 690, 776, 971, 1016
DpnI GATC 3 cut(s) 189, 207, 503
DpnII GATC 3 cut(s) 187, 205, 501
DraI TTTAAA 1 cut(s) 931
Eco105I TACGTA 1 cut(s) 572
Eco130I CCWWGG 3 cut(s) 366, 789, 960
Eco47I GGWCC 2 cut(s) 347, 877
Eco57I CTGAAG 3 cut(s) 468, 544, 872
Eco81I CCTNAGG 1 cut(s) 690
EcoT14I CCWWGG 3 cut(s) 366, 789, 960
ErhI CCWWGG 3 cut(s) 366, 789, 960
FaeI CATG 2 cut(s) 370, 1012
FaqI GGGAC 1 cut(s) 890
FatI CATG 2 cut(s) 366, 1008
FauNDI CATATG 1 cut(s) 183
FblI GTMKAC 2 cut(s) 356, 705
Fnu4HI GCNGC 3 cut(s) 318, 535, 894
FokI GGATG 2 cut(s) 665, 849
Fsp4HI GCNGC 3 cut(s) 318, 535, 894
FspBI CTAG 1 cut(s) 291
GlaI GCGC 2 cut(s) 531, 533
GluI GCNGC 3 cut(s) 318, 535, 894
HaeIII GGCC 1 cut(s) 965
HapII CCGG 1 cut(s) 890
HgaI GACGC 1 cut(s) 1021
HhaI GCGC 2 cut(s) 532, 534
Hin1II CATG 2 cut(s) 370, 1012
Hin6I GCGC 2 cut(s) 530, 532
HinP1I GCGC 2 cut(s) 530, 532
HinfI GANTC 2 cut(s) 154, 665
HpaII CCGG 1 cut(s) 890
HphI GGTGA 1 cut(s) 664
Hpy166II GTNNAC 4 cut(s) 357, 439, 556, 706
Hpy188I TCNGA 4 cut(s) 308, 501, 852, 1033
Hpy188III TCNNGA 3 cut(s) 158, 395, 1009
Hpy8I GTNNAC 4 cut(s) 357, 439, 556, 706
HpyAV CCTTC 3 cut(s) 575, 847, 987
HpyCH4III ACNGT 2 cut(s) 443, 886
HpyCH4IV ACGT 2 cut(s) 571, 746
HpyCH4V TGCA 7 cut(s) 123, 135, 317, 556, 620, 744, 896
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpyF3I CTNAG 7 cut(s) 116, 281, 351, 690, 776, 971, 1016
HpySE526I ACGT 2 cut(s) 571, 746
Hsp92II CATG 2 cut(s) 370, 1012
HspAI GCGC 2 cut(s) 530, 532
Kzo9I GATC 3 cut(s) 187, 205, 501
LmnI GCTCC 1 cut(s) 914
Lsp1109I GCAGC 2 cut(s) 329, 880
MaeI CTAG 1 cut(s) 291
MaeII ACGT 2 cut(s) 571, 746
MaeIII GTNAC 1 cut(s) 955
MalI GATC 3 cut(s) 189, 207, 503
MboI GATC 3 cut(s) 187, 205, 501
MboII GAAGA 1 cut(s) 806
MhlI GDGCHC 1 cut(s) 558
MluCI AATT 7 cut(s) 286, 382, 462, 606, 739, 834, 902
MmeI TCCRAC 2 cut(s) 224, 1011
MnlI CCTC 7 cut(s) 251, 515, 699, 760, 785, 867, 1013
MseI TTAA 3 cut(s) 381, 548, 930
MslI CAYNNNNRTG 1 cut(s) 561
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 1 cut(s) 890
Mva1269I GAATGC 2 cut(s) 532, 896
MvnI CGCG 3 cut(s) 396, 532, 534
MwoI GCNNNNNNNGC 1 cut(s) 337
NcoI CCATGG 1 cut(s) 366
NdeI CATATG 1 cut(s) 183
NdeII GATC 3 cut(s) 187, 205, 501
NlaIII CATG 2 cut(s) 370, 1012
NlaIV GGNNCC 1 cut(s) 878
NruI TCGCGA 1 cut(s) 396
NspV TTCGAA 2 cut(s) 11, 900
OliI CACNNNNGTG 1 cut(s) 561
PagI TCATGA 1 cut(s) 1008
PauI GCGCGC 1 cut(s) 530
PctI GAATGC 2 cut(s) 532, 896
PfeI GAWTC 2 cut(s) 154, 665
PkrI GCNGC 3 cut(s) 319, 536, 895
Ppu21I YACGTR 1 cut(s) 572
PsiI TTATAA 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 878
PspPI GGNCC 2 cut(s) 347, 877
PstI CTGCAG 1 cut(s) 319
PteI GCGCGC 1 cut(s) 530
RruI TCGCGA 1 cut(s) 396
RsaI GTAC 3 cut(s) 220, 445, 570
RsaNI GTAC 3 cut(s) 219, 444, 569
RseI CAYNNNNRTG 1 cut(s) 561
SaqAI TTAA 3 cut(s) 381, 548, 930
SatI GCNGC 3 cut(s) 318, 535, 894
Sau3AI GATC 3 cut(s) 187, 205, 501
Sau96I GGNCC 2 cut(s) 347, 877
ScaI AGTACT 1 cut(s) 445
SduI GDGCHC 1 cut(s) 558
SetI ASST 9 cut(s) 95, 122, 352, 574, 579, 711, 749, 923, 979
SfcI CTRYAG 1 cut(s) 315
SfuI TTCGAA 2 cut(s) 11, 900
SinI GGWCC 2 cut(s) 347, 877
SmiMI CAYNNNNRTG 1 cut(s) 561
SnaBI TACGTA 1 cut(s) 572
Sse9I AATT 7 cut(s) 286, 382, 462, 606, 739, 834, 902
SsiI CCGC 3 cut(s) 222, 534, 880
SspMI CTAG 1 cut(s) 291
StyI CCWWGG 3 cut(s) 366, 789, 960
TaaI ACNGT 2 cut(s) 443, 886
TaiI ACGT 2 cut(s) 574, 749
TaqI TCGA 3 cut(s) 11, 190, 900
TasI AATT 7 cut(s) 286, 382, 462, 606, 739, 834, 902
TatI WGTACW 1 cut(s) 443
TauI GCSGC 1 cut(s) 537
TfiI GAWTC 2 cut(s) 154, 665
Tru1I TTAA 3 cut(s) 381, 548, 930
Tru9I TTAA 3 cut(s) 381, 548, 930
TscAI CASTG 2 cut(s) 568, 889
TseI GCWGC 2 cut(s) 317, 893
TspDTI ATGAA 4 cut(s) 243, 375, 539, 581
TspRI CASTG 2 cut(s) 568, 889
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 2 cut(s) 347, 877
XapI RAATTY 1 cut(s) 834
XmiI GTMKAC 2 cut(s) 356, 705
XspI CTAG 1 cut(s) 291
ZrmI AGTACT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.