Rh5CG043100

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000620
Physical Location & Seq
Reverse (-)
2974587 .. 2975405
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG043100.1

Sequence Viewer

Length: 819 bp
ATGACTGAAGAAGTGTATGAGCTTCTTTTCGATGTTATCCTTGAGATTTTGACTTGGTTGCCAGTAAAGTCTCTACTAAGATTTAAGTGTGTCTGCAAGCAATGGTGTTCCTTGATCAAGGAAGATCGCAAGTTCATCGCAAAACATATGGATCGGGCTACTCCTCTCCAACTTACTCATCTGCATAAATGCGGAGATTTCTACAAAGTGGACTATGGCTATGGATTATATCTGGAAAAAAGTGATGATTTTGGCTGTCGTATCAGAAATCCTGCAACCCAACAAGTACTTTACTTACCCTATGCAGATGAGGATATTGTCCTACTACGTTTTTATTATGATTCAGTCACCGATGAGTGTAAAGTGCTACGTCGTCGCAATTCAGATAATGTTTTATCATATACATTTGAAATTATAACAATTGGAAAGGATGACCAATGGAGACCCCTATATAATATTGGCAAATCACCAAGGTCTGTACATTTTTATGGAGCAGATGAGGGTTCAGATTGGTTATATAACGACTTTAATACTGTACCCCCAGAAATTCATTCTTGTGATACGAGGAGGGAATGTATCACGACTAATACTGTACCCCCAGGAGCTTTTACAGATTTGACAAAAGTATCGGTTCTCCTTTGGAACGATCGTCTCGCTGTTGGTGATATAGCAAATGAAGCCCTAAATGTCTTGGTGTTAGAGGACTTCAAAGAGCACAAATGGAGCAAAAACAAGATCGTTGTTCCCTTGAAATTTTCGAAGGATGACCCTCTTTTCCAGAAGAATTGGAGTATTCCTTGGTCATTTGATTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

272

Amino Acids

31.89

Weight (kDa)

5.47

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 10 - 45 5.2e-09 F-box domain
F-box-like PF12937 11 - 42 2e-06 F-box-like
FBA_3 PF08268 79 - 253 5.3e-13 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
AciI CCGC 1 cut(s) 192
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 2 cut(s) 546, 752
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 4 cut(s) 288, 480, 537, 594
AgsI TTSAA 3 cut(s) 410, 709, 751
AjnI CCWGG 1 cut(s) 598
AluBI AGCT 2 cut(s) 22, 605
AluI AGCT 2 cut(s) 22, 605
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 3 cut(s) 75, 436, 656
AlwI GGATC 1 cut(s) 159
ApoI RAATTY 2 cut(s) 546, 752
AsuHPI GGTGA 3 cut(s) 340, 459, 674
AsuII TTCGAA 1 cut(s) 758
BaeI ACNNNNGTAYC 2 cut(s) 609, 642
Bbv12I GWGCWC 1 cut(s) 717
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 600
BclI TGATCA 1 cut(s) 114
BcoDI GTCTC 3 cut(s) 75, 436, 656
BmcAI AGTACT 1 cut(s) 288
Bme1390I CCNGG 1 cut(s) 600
BmrFI CCNGG 1 cut(s) 600
Bpu14I TTCGAA 1 cut(s) 758
BpuEI CTTGAG 1 cut(s) 62
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 3 cut(s) 470, 598, 797
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bse1I ACTGG 1 cut(s) 62
Bse3DI GCAATG 1 cut(s) 107
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 3 cut(s) 470, 598, 797
BseGI GGATG 2 cut(s) 436, 769
BseMI GCAATG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 2 cut(s) 153, 580
Bsh1285I CGRYCG 1 cut(s) 649
BsiEI CGRYCG 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 717
BsmAI GTCTC 3 cut(s) 75, 436, 656
BsmBI CGTCTC 1 cut(s) 656
Bso31I GGTCTC 1 cut(s) 436
Bsp119I TTCGAA 1 cut(s) 758
Bsp1286I GDGCHC 1 cut(s) 717
Bsp1407I TGTACA 1 cut(s) 478
Bsp143I GATC 5 cut(s) 114, 124, 151, 646, 735
BspACI CCGC 1 cut(s) 192
BspCNI CTCAG 1 cut(s) 805
BspPI GGATC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 758
BspTNI GGTCTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 107
BsrGI TGTACA 1 cut(s) 478
BsrI ACTGG 1 cut(s) 62
BssECI CCNNGG 3 cut(s) 470, 598, 797
BssMI GATC 5 cut(s) 114, 124, 151, 646, 735
BssT1I CCWWGG 2 cut(s) 470, 797
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 2 cut(s) 535, 592
BstAUI TGTACA 1 cut(s) 478
BstBI TTCGAA 1 cut(s) 758
BstC8I GCNNGC 1 cut(s) 98
BstDEI CTNAG 2 cut(s) 77, 813
BstF5I GGATG 2 cut(s) 436, 769
BstKTI GATC 5 cut(s) 117, 127, 154, 649, 738
BstMAI GTCTC 3 cut(s) 75, 436, 656
BstMBI GATC 5 cut(s) 114, 124, 151, 646, 735
BstMCI CGRYCG 1 cut(s) 649
BstMWI GCNNNNNNNGC 1 cut(s) 677
BstNI CCWGG 1 cut(s) 600
BstSCI CCNGG 1 cut(s) 598
BtgZI GCGATG 1 cut(s) 121
BtsCI GGATG 2 cut(s) 436, 769
Cac8I GCNNGC 1 cut(s) 98
Csp6I GTAC 4 cut(s) 287, 479, 536, 593
CviJI RGCY 6 cut(s) 22, 158, 219, 255, 605, 680
CviKI_1 RGCY 6 cut(s) 22, 158, 219, 255, 605, 680
CviQI GTAC 4 cut(s) 287, 479, 536, 593
DdeI CTNAG 2 cut(s) 77, 813
DpnI GATC 5 cut(s) 116, 126, 153, 648, 737
DpnII GATC 5 cut(s) 114, 124, 151, 646, 735
Eco130I CCWWGG 2 cut(s) 470, 797
Eco31I GGTCTC 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 598
EcoT14I CCWWGG 2 cut(s) 470, 797
ErhI CCWWGG 2 cut(s) 470, 797
Esp3I CGTCTC 1 cut(s) 656
FauNDI CATATG 1 cut(s) 147
FbaI TGATCA 1 cut(s) 114
FokI GGATG 2 cut(s) 443, 776
HinfI GANTC 2 cut(s) 341, 809
HphI GGTGA 3 cut(s) 340, 459, 674
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 4 cut(s) 266, 385, 508, 814
Hpy188III TCNNGA 3 cut(s) 233, 580, 778
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 2 cut(s) 375, 378
HpyAV CCTTC 1 cut(s) 754
HpyCH4III ACNGT 2 cut(s) 535, 592
HpyCH4IV ACGT 2 cut(s) 328, 370
HpyCH4V TGCA 4 cut(s) 96, 184, 275, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 677
HpyF3I CTNAG 2 cut(s) 77, 813
HpySE526I ACGT 2 cut(s) 328, 370
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 5 cut(s) 114, 124, 151, 646, 735
LmnI GCTCC 3 cut(s) 491, 602, 723
LpnPI CCDG 7 cut(s) 75, 218, 285, 555, 585, 612, 791
MaeII ACGT 2 cut(s) 328, 370
MaeIII GTNAC 1 cut(s) 346
MalI GATC 5 cut(s) 116, 126, 153, 648, 737
MboI GATC 5 cut(s) 114, 124, 151, 646, 735
MboII GAAGA 3 cut(s) 20, 134, 793
MfeI CAATTG 1 cut(s) 420
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 6 cut(s) 379, 411, 420, 546, 752, 784
MmeI TCCRAC 1 cut(s) 193
MnlI CCTC 7 cut(s) 174, 304, 493, 558, 561, 694, 780
MseI TTAA 2 cut(s) 84, 528
MslI CAYNNNNRTG 2 cut(s) 486, 555
MspR9I CCNGG 1 cut(s) 600
MunI CAATTG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 600
MwoI GCNNNNNNNGC 1 cut(s) 677
NdeI CATATG 1 cut(s) 147
NdeII GATC 5 cut(s) 114, 124, 151, 646, 735
NmuCI GTSAC 1 cut(s) 346
NspV TTCGAA 1 cut(s) 758
PfeI GAWTC 2 cut(s) 341, 809
Ple19I CGATCG 1 cut(s) 649
PsiI TTATAA 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PvuI CGATCG 1 cut(s) 649
RsaI GTAC 4 cut(s) 288, 480, 537, 594
RsaNI GTAC 4 cut(s) 287, 479, 536, 593
RseI CAYNNNNRTG 2 cut(s) 486, 555
SaqAI TTAA 2 cut(s) 84, 528
Sau3AI GATC 5 cut(s) 114, 124, 151, 646, 735
ScaI AGTACT 1 cut(s) 288
ScrFI CCNGG 1 cut(s) 600
SduI GDGCHC 1 cut(s) 717
SetI ASST 5 cut(s) 24, 331, 373, 476, 607
SfuI TTCGAA 1 cut(s) 758
SmiMI CAYNNNNRTG 2 cut(s) 486, 555
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 6 cut(s) 379, 411, 420, 546, 752, 784
SsiI CCGC 1 cut(s) 192
SspI AATATT 1 cut(s) 457
StyD4I CCNGG 1 cut(s) 598
StyI CCWWGG 2 cut(s) 470, 797
TaaI ACNGT 2 cut(s) 535, 592
TaiI ACGT 2 cut(s) 331, 373
TaqI TCGA 2 cut(s) 30, 758
TasI AATT 6 cut(s) 379, 411, 420, 546, 752, 784
TatI WGTACW 2 cut(s) 286, 478
TfiI GAWTC 2 cut(s) 341, 809
Tru1I TTAA 2 cut(s) 84, 528
Tru9I TTAA 2 cut(s) 84, 528
TseFI GTSAC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 3 cut(s) 124, 539, 690
XapI RAATTY 2 cut(s) 546, 752
ZrmI AGTACT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.