pycom05g30760

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000620
Physical Location & Seq
Forward (+)
30748304 .. 30749239
936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30760.1

Sequence Viewer

Length: 936 bp
ATGGGAGTCCAACGTGTCGCTACCAAAGAAGAAGAAGAAGTGCCGCGGCAACAATCACGAGAGCTTCCCTTTGATATTGTTGCTCAGATTTTGTTGTGGCTGCCTGTGGTGTCTTTGGTGAGATTCAAGTGTGTTTGCAAGCAATGGTGTCTGCTAATTGAGGGCTACAAGTTCATAGAAAAGCATAGGGCTCAAACACGTTACAGTTTGCCACCTTGTTATCAATGCCAATGGGATAAGGGGAGAATGAAGGGAGTTGCTTCCTATGGGGATTTCGAGCTTCAGTGTGATTGTAGTGGCTTGTTTTTGGAGAAGAGTAAAACTTCTCAAGTTTGCCGTATTAGAAATCCTGCAACCCACAAAGTGCTCTACTTGCCCAAGGCACCCGAGGGTACCAGAGTAGTTCTGGACCTCGGTTTTAATTCCCTTACTGGTGAGTGTAAAGTGGCCTGTGCTTATCGCCGAGAGGGCAGTTTAGTAGGTGTTGATGTTATAACAATTGGAAAGGATGAAATGTGGAGACCCTTGCTCCGCCAAAAGCTGAATTTTCTGGAACAAGGCAGATGGGTGGTAAAGCCAAATTGTTGTGAGGAAGATAAGGTTAACTGGGCTATTTATATCTCCAAAATTGTAAAAGATGAACATGATGATCATTCGTGCCTACAAATTCAGTCTCTTGATCTGTGGAGTGAATGTCTCACCACAACTACTTTGCCCCAGGGAGTTTTCCTAAATTTGAGGAATGTTCGGGCTTTTCCTTGGGATCTCTGTCTAGGCGTTGCTGAAACAGTAGGGGGAGCCATTCACATCTTGGTGTTAGAAGACTTCAAGGAACACAAATGGAGTCGAAACAAGATCGATCATTCCTTTGAAAAATTTAAAGGTCGACCACACAGTTTGGACGGATCATTTAGTTCTCACACAAGCTCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

312

Amino Acids

35.74

Weight (kDa)

8.3

Isoelectric Point (pI)

48.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 21 - 58 2.3e-08 F-box domain
FBA_3 PF08268 98 - 289 3.1e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 2 cut(s) 382, 392
AccI GTMKAC 1 cut(s) 886
AccII CGCG 1 cut(s) 46
AciI CCGC 3 cut(s) 44, 46, 532
AclWI GGATC 2 cut(s) 771, 913
AcsI RAATTY 4 cut(s) 544, 666, 733, 875
AcuI CTGAAG 1 cut(s) 266
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 1 cut(s) 394
AflIII ACRYGT 2 cut(s) 13, 197
AgsI TTSAA 3 cut(s) 127, 829, 872
AjnI CCWGG 1 cut(s) 717
AluBI AGCT 4 cut(s) 64, 280, 541, 927
AluI AGCT 4 cut(s) 64, 280, 541, 927
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 3 cut(s) 514, 678, 701
AlwI GGATC 2 cut(s) 771, 913
Ama87I CYCGRG 1 cut(s) 386
AoxI GGCC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 4 cut(s) 544, 666, 733, 875
Asp718I GGTACC 1 cut(s) 392
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 3 cut(s) 130, 446, 691
AvaI CYCGRG 1 cut(s) 386
AvaII GGWCC 1 cut(s) 409
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BanI GGYRCC 2 cut(s) 382, 392
BanII GRGCYC 1 cut(s) 193
BauI CACGAG 1 cut(s) 57
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 87
BccI CCATC 1 cut(s) 558
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 1 cut(s) 719
BclI TGATCA 1 cut(s) 649
BcoDI GTCTC 3 cut(s) 514, 678, 701
BfaI CTAG 2 cut(s) 773, 934
BglI GCCNNNNNGGC 1 cut(s) 468
BisI GCNGC 3 cut(s) 44, 47, 101
BlsI GCNGC 3 cut(s) 45, 48, 102
Bme1390I CCNGG 1 cut(s) 719
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 409
BmiI GGNNCC 3 cut(s) 384, 394, 799
BmrFI CCNGG 1 cut(s) 719
BmrI ACTGGG 1 cut(s) 616
BmuI ACTGGG 1 cut(s) 616
BpiI GAAGAC 1 cut(s) 828
BpuEI CTTGAG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 7 cut(s) 44, 378, 387, 412, 717, 718, 758
BsaXI ACNNNNNCTCC 4 cut(s) 513, 543, 679, 709
Bse1I ACTGG 2 cut(s) 436, 611
Bse3DI GCAATG 1 cut(s) 149
BseBI CCWGG 1 cut(s) 719
BseCI ATCGAT 1 cut(s) 858
BseDI CCNNGG 7 cut(s) 44, 378, 387, 412, 717, 718, 758
BseGI GGATG 1 cut(s) 514
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 436, 611
BseXI GCAGC 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 1 cut(s) 449
BshNI GGYRCC 2 cut(s) 382, 392
BshVI ATCGAT 1 cut(s) 858
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 386
BsmAI GTCTC 3 cut(s) 514, 678, 701
BsnI GGCC 1 cut(s) 449
Bso31I GGTCTC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 386
Bsp1286I GDGCHC 2 cut(s) 193, 369
Bsp143I GATC 6 cut(s) 649, 679, 763, 855, 859, 905
BspACI CCGC 3 cut(s) 44, 46, 532
BspANI GGCC 1 cut(s) 449
BspCNI CTCAG 1 cut(s) 97
BspDI ATCGAT 1 cut(s) 858
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 384, 394, 799
BspPI GGATC 2 cut(s) 771, 913
BspT107I GGYRCC 2 cut(s) 382, 392
BspTNI GGTCTC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 149
BsrI ACTGG 2 cut(s) 436, 611
BssECI CCNNGG 7 cut(s) 44, 378, 387, 412, 717, 718, 758
BssMI GATC 6 cut(s) 649, 679, 763, 855, 859, 905
BssSI CACGAG 1 cut(s) 57
BssT1I CCWWGG 2 cut(s) 378, 758
Bst2BI CACGAG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 719
Bst4CI ACNGT 3 cut(s) 206, 790, 896
Bst6I CTCTTC 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 1 cut(s) 84
BstDSI CCRYGG 1 cut(s) 44
BstF5I GGATG 1 cut(s) 514
BstFNI CGCG 1 cut(s) 46
BstKTI GATC 6 cut(s) 652, 682, 766, 858, 862, 908
BstMAI GTCTC 3 cut(s) 514, 678, 701
BstMBI GATC 6 cut(s) 649, 679, 763, 855, 859, 905
BstMWI GCNNNNNNNGC 2 cut(s) 373, 468
BstNI CCWGG 1 cut(s) 719
BstSCI CCNGG 1 cut(s) 717
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 1 cut(s) 87
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 1 cut(s) 763
BstYI RGATCY 1 cut(s) 763
Bsu15I ATCGAT 1 cut(s) 858
BsuRI GGCC 1 cut(s) 449
BsuTUI ATCGAT 1 cut(s) 858
BtgI CCRYGG 1 cut(s) 44
BtsCI GGATG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 409
Cfr42I CCGCGG 1 cut(s) 47
ClaI ATCGAT 1 cut(s) 858
Csp6I GTAC 1 cut(s) 393
CspCI CAANNNNNGTGG 2 cut(s) 879, 914
CviAII CATG 1 cut(s) 644
CviQI GTAC 1 cut(s) 393
DdeI CTNAG 1 cut(s) 84
DpnI GATC 6 cut(s) 651, 681, 765, 857, 861, 907
DpnII GATC 6 cut(s) 649, 679, 763, 855, 859, 905
DraI TTTAAA 1 cut(s) 880
DraIII CACNNNGTG 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 1 cut(s) 521
Eco130I CCWWGG 2 cut(s) 378, 758
Eco24I GRGCYC 1 cut(s) 193
Eco31I GGTCTC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 717
EcoT14I CCWWGG 2 cut(s) 378, 758
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 2 cut(s) 378, 758
FaeI CATG 1 cut(s) 647
FaiI YATR 6 cut(s) 176, 186, 267, 494, 618, 645
FatI CATG 1 cut(s) 643
FbaI TGATCA 1 cut(s) 649
FblI GTMKAC 1 cut(s) 886
Fnu4HI GCNGC 3 cut(s) 44, 47, 101
FokI GGATG 1 cut(s) 521
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 3 cut(s) 44, 47, 101
FspBI CTAG 2 cut(s) 773, 934
GluI GCNGC 3 cut(s) 44, 47, 101
HaeIII GGCC 1 cut(s) 449
Hin1II CATG 1 cut(s) 647
HincII GTYRAC 2 cut(s) 604, 887
HindII GTYRAC 2 cut(s) 604, 887
HinfI GANTC 3 cut(s) 6, 123, 844
HpaI GTTAAC 1 cut(s) 604
HphI GGTGA 3 cut(s) 130, 446, 691
Hpy166II GTNNAC 2 cut(s) 604, 887
Hpy188I TCNGA 1 cut(s) 87
Hpy188III TCNNGA 4 cut(s) 57, 407, 551, 677
Hpy8I GTNNAC 2 cut(s) 604, 887
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 3 cut(s) 206, 790, 896
HpyCH4IV ACGT 2 cut(s) 13, 199
HpyCH4V TGCA 2 cut(s) 138, 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 373, 468
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 2 cut(s) 13, 199
Hsp92II CATG 1 cut(s) 647
KpnI GGTACC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 649
KspAI GTTAAC 1 cut(s) 604
KspI CCGCGG 1 cut(s) 47
Kzo9I GATC 6 cut(s) 649, 679, 763, 855, 859, 905
LmnI GCTCC 2 cut(s) 534, 797
Lsp1109I GCAGC 1 cut(s) 87
MaeI CTAG 2 cut(s) 773, 934
MaeII ACGT 2 cut(s) 13, 199
MaeIII GTNAC 1 cut(s) 200
MalI GATC 6 cut(s) 651, 681, 765, 857, 861, 907
MboI GATC 6 cut(s) 649, 679, 763, 855, 859, 905
MboII GAAGA 6 cut(s) 41, 44, 47, 325, 605, 833
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 2 cut(s) 193, 369
MluCI AATT 9 cut(s) 156, 421, 498, 544, 580, 627, 666, 733, 875
MlyI GAGTC 2 cut(s) 15, 853
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 6 cut(s) 154, 382, 422, 460, 583, 732
MseI TTAA 3 cut(s) 420, 603, 879
MslI CAYNNNNRTG 1 cut(s) 812
MspA1I CMGCKG 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 719
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 719
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 373, 468
NdeII GATC 6 cut(s) 649, 679, 763, 855, 859, 905
NlaIII CATG 1 cut(s) 647
NlaIV GGNNCC 3 cut(s) 384, 394, 799
NmeAIII GCCGAG 1 cut(s) 488
PasI CCCWGGG 1 cut(s) 718
PfeI GAWTC 1 cut(s) 123
PkrI GCNGC 3 cut(s) 45, 48, 102
PleI GAGTC 2 cut(s) 14, 852
PpsI GAGTC 2 cut(s) 14, 852
PsiI TTATAA 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 717
PspGI CCWGG 1 cut(s) 717
PspN4I GGNNCC 3 cut(s) 384, 394, 799
PspPI GGNCC 1 cut(s) 409
PsuI RGATCY 1 cut(s) 763
RsaI GTAC 1 cut(s) 394
RsaNI GTAC 1 cut(s) 393
RseI CAYNNNNRTG 1 cut(s) 812
SacII CCGCGG 1 cut(s) 47
SalI GTCGAC 1 cut(s) 885
SaqAI TTAA 3 cut(s) 420, 603, 879
SatI GCNGC 3 cut(s) 44, 47, 101
Sau3AI GATC 6 cut(s) 649, 679, 763, 855, 859, 905
Sau96I GGNCC 1 cut(s) 409
SchI GAGTC 2 cut(s) 15, 853
ScrFI CCNGG 1 cut(s) 719
SduI GDGCHC 2 cut(s) 193, 369
Sfr303I CCGCGG 1 cut(s) 47
SgrBI CCGCGG 1 cut(s) 47
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 9 cut(s) 156, 421, 498, 544, 580, 627, 666, 733, 875
SsiI CCGC 3 cut(s) 44, 46, 532
SspMI CTAG 2 cut(s) 773, 934
StyD4I CCNGG 1 cut(s) 717
StyI CCWWGG 2 cut(s) 378, 758
TaaI ACNGT 3 cut(s) 206, 790, 896
TaiI ACGT 2 cut(s) 16, 202
TaqI TCGA 4 cut(s) 276, 847, 858, 886
TasI AATT 9 cut(s) 156, 421, 498, 544, 580, 627, 666, 733, 875
TauI GCSGC 2 cut(s) 46, 49
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 3 cut(s) 420, 603, 879
Tru9I TTAA 3 cut(s) 420, 603, 879
TscAI CASTG 1 cut(s) 290
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 4 cut(s) 163, 263, 525, 654
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 4 cut(s) 544, 666, 733, 875
XcmI CCANNNNNNNNNTGG 2 cut(s) 403, 808
XmiI GTMKAC 1 cut(s) 886
XspI CTAG 2 cut(s) 773, 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.