MD10G1212400.v1.1

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000620
Physical Location & Seq
Reverse (-)
31103544 .. 31104674
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1212400.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGGCGCACGGCTACCACCTGAGAAGTAGGAGGAGGAGGAGGAGATTGGACCCCAAACCAGAAGAACCAAAGCTTCCCTTTGAAATCATTATTGAGATTCTGAGCTGGTTGCCTGTAGAGGCATTACTCCGGTTCAAGTGTGTCAGCAAAACATGGTGCTCTTTGATTGAAGAATACAACTTCATTTTAAAGCACATGAAACGGGTCAGTCCTATCCATCTACAGTTTCGACTAAAAAGCGAAAAGAACCATGTAGCCCCTTACTTTGATTCAAATTACGAGGGCATGGGACGTGTTGCTGGGATGTTTCTGGAGAAGCATATTACTACTCACCTTTGCCGCATCAGAAATCCGGCAACCCGCCGAGTACTCAACTTGCCTGAAGCTCATAAGGGTGCGAGAAGAATGTCATCTGCTTTCAATTTATCGACTGGTGAGATCAAAGTGGTAACTTTATATGTCGAGAACAATGAATTAGGGTTTCAAATTATGACCATTGGGAAAGATGAAAAGTGGAGACCCTGGACGCACCCAAATCAGGGTTTGCTCAAGGAACAAGGCAGAAGGGTAGTGAGATGTAATTTTTTTGCAGCTGAAAAGATGAATGGTAGGGTTTGTTATTCTGCTGAGTTTATGAAAAGAGATGGCCAAGATCTGTCAATATTAGAAGTCCAACGCCTTGATCTGGTGGATGAGTCTGTCTTAACAGCAACCCTACCTCAGGGAGTTTTTCCCGATTTAAACAAAGTTTGCGTCATCAATTGGAACAAGCATATGGCTGTGGTTGAGATAGTAAATGCCACCCTTCATGCCTTGGTGTGTGAAGACTCCAAGGAGAACAAATGGAGCCCGAAAAAGATCGTTGTTCCGTTGAAGTTTATGGAGAAAATACTTCCACCTCTTCAGGAAGGTCGAGATGTGATCATTCCGGTGAATGTTGAAGGTGATGACTTCAGTTTCTATTGTTTTCAGGGAGAACAGTCTTTTGTGTACAACACGAAGATGCAGAAGGCTAAGTATTTATACTCTGGTGTGGATCGTTGGACTGCTCATAGGCCAAGCTTGATGGGCTTTAAGGGAATGAGGCTGGAAGAGGAAGCAACTACAAGTGCAGCTAAGATTGAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

377

Amino Acids

43.67

Weight (kDa)

9.25

Isoelectric Point (pI)

57.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 24 - 61 2e-09 F-box domain
F-box-like PF12937 24 - 57 3.3e-07 F-box-like
FBA_3 PF08268 93 - 295 3.7e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 340, 361
AclWI GGATC 1 cut(s) 1044
AcoI YGGCCR 1 cut(s) 646
AcuI CTGAAG 3 cut(s) 402, 887, 937
AfaI GTAC 2 cut(s) 369, 992
AfiI CCNNNNNNNGG 4 cut(s) 538, 539, 685, 721
AflIII ACRYGT 1 cut(s) 292
AgsI TTSAA 8 cut(s) 83, 136, 170, 273, 421, 485, 874, 941
AjiI CACGTC 1 cut(s) 293
AjnI CCWGG 1 cut(s) 521
AluBI AGCT 6 cut(s) 73, 105, 386, 593, 1062, 1115
AluI AGCT 6 cut(s) 73, 105, 386, 593, 1062, 1115
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 1 cut(s) 511
AlwI GGATC 1 cut(s) 1044
AoxI GGCC 2 cut(s) 646, 1055
ApeKI GCWGC 2 cut(s) 590, 1112
ArsI GACNNNNNNTTYG 2 cut(s) 738, 770
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 4 cut(s) 323, 446, 943, 956
AvaII GGWCC 1 cut(s) 49
AxyI CCTNAGG 1 cut(s) 720
BalI TGGCCA 1 cut(s) 648
BanII GRGCYC 1 cut(s) 851
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 161
BbvI GCAGC 2 cut(s) 602, 1124
BccI CCATC 3 cut(s) 225, 638, 1060
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 1 cut(s) 523
BclI TGATCA 1 cut(s) 921
BcoDI GTCTC 1 cut(s) 511
BfmI CTRYAG 2 cut(s) 114, 221
BglII AGATCT 1 cut(s) 652
BisI GCNGC 3 cut(s) 340, 591, 1113
BlsI GCNGC 3 cut(s) 341, 592, 1114
BmcAI AGTACT 1 cut(s) 369
Bme1390I CCNGG 1 cut(s) 523
Bme18I GGWCC 1 cut(s) 49
BmgBI CACGTC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 2 cut(s) 51, 848
BmrFI CCNGG 1 cut(s) 523
BmsI GCATC 2 cut(s) 351, 993
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 533
BsaI GGTCTC 1 cut(s) 511
BsaJI CCNNGG 3 cut(s) 521, 813, 831
BsaWI WCCGGW 2 cut(s) 129, 928
Bsc4I CCNNNNNNNGG 4 cut(s) 538, 539, 685, 721
Bse1I ACTGG 1 cut(s) 436
Bse21I CCTNAGG 1 cut(s) 720
BseBI CCWGG 1 cut(s) 523
BseDI CCNNGG 3 cut(s) 521, 813, 831
BseGI GGATG 2 cut(s) 309, 697
BseLI CCNNNNNNNGG 4 cut(s) 538, 539, 685, 721
BseMII CTCAG 4 cut(s) 11, 92, 618, 734
BseNI ACTGG 1 cut(s) 436
BseRI GAGGAG 4 cut(s) 46, 49, 52, 55
BseXI GCAGC 2 cut(s) 602, 1124
BseYI CCCAGC 1 cut(s) 299
BsgI GTGCAG 1 cut(s) 1131
BshFI GGCC 2 cut(s) 648, 1057
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 3 cut(s) 130, 353, 929
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 4 cut(s) 538, 539, 685, 721
BsmAI GTCTC 1 cut(s) 511
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 2 cut(s) 648, 1057
Bso31I GGTCTC 1 cut(s) 511
Bsp1286I GDGCHC 2 cut(s) 161, 851
Bsp1407I TGTACA 1 cut(s) 990
Bsp143I GATC 6 cut(s) 438, 652, 682, 858, 921, 1036
BspACI CCGC 2 cut(s) 340, 361
BspANI GGCC 2 cut(s) 648, 1057
BspCNI CTCAG 4 cut(s) 12, 93, 619, 733
BspLI GGNNCC 2 cut(s) 51, 848
BspPI GGATC 1 cut(s) 1044
BspTNI GGTCTC 1 cut(s) 511
BsrGI TGTACA 1 cut(s) 990
BsrI ACTGG 1 cut(s) 436
BssECI CCNNGG 3 cut(s) 521, 813, 831
BssMI GATC 6 cut(s) 438, 652, 682, 858, 921, 1036
BssT1I CCWWGG 2 cut(s) 813, 831
Bst2UI CCWGG 1 cut(s) 523
Bst4CI ACNGT 2 cut(s) 225, 981
Bst6I CTCTTC 2 cut(s) 906, 1086
BstAUI TGTACA 1 cut(s) 990
BstDEI CTNAG 6 cut(s) 20, 101, 627, 720, 1014, 1116
BstENI CCTNNNNNAGG 1 cut(s) 719
BstF5I GGATG 2 cut(s) 309, 697
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 6 cut(s) 441, 655, 685, 861, 924, 1039
BstMAI GTCTC 1 cut(s) 511
BstMBI GATC 6 cut(s) 438, 652, 682, 858, 921, 1036
BstMWI GCNNNNNNNGC 1 cut(s) 1068
BstNI CCWGG 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 521
BstSFI CTRYAG 2 cut(s) 114, 221
BstV1I GCAGC 2 cut(s) 602, 1124
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 1 cut(s) 652
BstYI RGATCY 1 cut(s) 652
Bsu36I CCTNAGG 1 cut(s) 720
BsuRI GGCC 2 cut(s) 648, 1057
BtrI CACGTC 1 cut(s) 293
BtsCI GGATG 2 cut(s) 309, 697
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 49
CseI GACGC 2 cut(s) 535, 742
Csp6I GTAC 2 cut(s) 368, 991
CviAII CATG 5 cut(s) 153, 196, 251, 286, 809
CviQI GTAC 2 cut(s) 368, 991
DdeI CTNAG 6 cut(s) 20, 101, 627, 720, 1014, 1116
DpnI GATC 6 cut(s) 440, 654, 684, 860, 923, 1038
DpnII GATC 6 cut(s) 438, 652, 682, 858, 921, 1036
DraI TTTAAA 2 cut(s) 189, 741
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 2 cut(s) 906, 1086
EarI CTCTTC 2 cut(s) 906, 1086
Eco130I CCWWGG 2 cut(s) 813, 831
Eco24I GRGCYC 1 cut(s) 851
Eco31I GGTCTC 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 3 cut(s) 402, 887, 937
Eco81I CCTNAGG 1 cut(s) 720
EcoNI CCTNNNNNAGG 1 cut(s) 719
EcoRII CCWGG 1 cut(s) 521
EcoT14I CCWWGG 2 cut(s) 813, 831
EcoT38I GRGCYC 1 cut(s) 851
ErhI CCWWGG 2 cut(s) 813, 831
FaeI CATG 5 cut(s) 156, 199, 254, 289, 812
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FaqI GGGAC 1 cut(s) 303
FatI CATG 5 cut(s) 152, 195, 250, 285, 808
FauI CCCGC 1 cut(s) 368
FauNDI CATATG 1 cut(s) 774
FbaI TGATCA 1 cut(s) 921
Fnu4HI GCNGC 3 cut(s) 340, 591, 1113
FokI GGATG 2 cut(s) 316, 704
FriOI GRGCYC 1 cut(s) 851
Fsp4HI GCNGC 3 cut(s) 340, 591, 1113
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 340, 591, 1113
GsaI CCCAGC 1 cut(s) 303
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 2 cut(s) 648, 1057
HapII CCGG 3 cut(s) 130, 353, 929
HgaI GACGC 2 cut(s) 535, 742
HhaI GCGC 1 cut(s) 7
Hin1II CATG 5 cut(s) 156, 199, 254, 289, 812
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HindIII AAGCTT 2 cut(s) 71, 1060
HinfI GANTC 4 cut(s) 97, 269, 695, 827
HpaII CCGG 3 cut(s) 130, 353, 929
HphI GGTGA 4 cut(s) 323, 446, 943, 956
Hpy166II GTNNAC 1 cut(s) 991
Hpy188I TCNGA 2 cut(s) 102, 347
Hpy188III TCNNGA 5 cut(s) 311, 463, 734, 905, 914
Hpy8I GTNNAC 1 cut(s) 991
HpyAV CCTTC 5 cut(s) 558, 815, 902, 935, 1003
HpyCH4III ACNGT 2 cut(s) 225, 981
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 3 cut(s) 590, 1006, 1112
HpyF10VI GCNNNNNNNGC 1 cut(s) 1068
HpyF3I CTNAG 6 cut(s) 20, 101, 627, 720, 1014, 1116
HpySE526I ACGT 1 cut(s) 292
Hsp92II CATG 5 cut(s) 156, 199, 254, 289, 812
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 921
Kzo9I GATC 6 cut(s) 438, 652, 682, 858, 921, 1036
LmnI GCTCC 1 cut(s) 846
Lsp1109I GCAGC 2 cut(s) 602, 1124
LweI GCATC 2 cut(s) 351, 993
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 448
MalI GATC 6 cut(s) 440, 654, 684, 860, 923, 1038
MboI GATC 6 cut(s) 438, 652, 682, 858, 921, 1036
MboII GAAGA 7 cut(s) 74, 182, 414, 836, 893, 1012, 1103
MfeI CAATTG 1 cut(s) 760
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 2 cut(s) 161, 851
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 6 cut(s) 274, 421, 473, 486, 580, 760
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 2 cut(s) 704, 821
MmeI TCCRAC 2 cut(s) 697, 1022
Mox20I TGGCCA 1 cut(s) 648
MscI TGGCCA 1 cut(s) 648
MseI TTAA 4 cut(s) 188, 704, 740, 1074
MslI CAYNNNNRTG 3 cut(s) 393, 929, 1001
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 3 cut(s) 130, 353, 929
MspR9I CCNGG 1 cut(s) 523
MunI CAATTG 1 cut(s) 760
MvaI CCWGG 1 cut(s) 523
MwoI GCNNNNNNNGC 1 cut(s) 1068
NdeI CATATG 1 cut(s) 774
NdeII GATC 6 cut(s) 438, 652, 682, 858, 921, 1036
NlaIII CATG 5 cut(s) 156, 199, 254, 289, 812
NlaIV GGNNCC 2 cut(s) 51, 848
NmeAIII GCCGAG 1 cut(s) 389
PfeI GAWTC 2 cut(s) 97, 269
PkrI GCNGC 3 cut(s) 341, 592, 1114
PleI GAGTC 2 cut(s) 703, 821
PpsI GAGTC 2 cut(s) 703, 821
Psp6I CCWGG 1 cut(s) 521
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 1 cut(s) 521
PspN4I GGNNCC 2 cut(s) 51, 848
PspPI GGNCC 1 cut(s) 49
PsuI RGATCY 1 cut(s) 652
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 2 cut(s) 369, 992
RsaNI GTAC 2 cut(s) 368, 991
RseI CAYNNNNRTG 3 cut(s) 393, 929, 1001
SaqAI TTAA 4 cut(s) 188, 704, 740, 1074
SatI GCNGC 3 cut(s) 340, 591, 1113
Sau3AI GATC 6 cut(s) 438, 652, 682, 858, 921, 1036
Sau96I GGNCC 1 cut(s) 49
ScaI AGTACT 1 cut(s) 369
SchI GAGTC 2 cut(s) 704, 821
ScrFI CCNGG 1 cut(s) 523
SduI GDGCHC 2 cut(s) 161, 851
SfaNI GCATC 2 cut(s) 351, 993
SfcI CTRYAG 2 cut(s) 114, 221
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 3 cut(s) 393, 929, 1001
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 6 cut(s) 274, 421, 473, 486, 580, 760
SsiI CCGC 2 cut(s) 340, 361
SspI AATATT 1 cut(s) 663
StyD4I CCNGG 1 cut(s) 521
StyI CCWWGG 2 cut(s) 813, 831
TaaI ACNGT 2 cut(s) 225, 981
TaiI ACGT 1 cut(s) 295
TaqI TCGA 4 cut(s) 229, 428, 462, 913
TasI AATT 6 cut(s) 274, 421, 473, 486, 580, 760
TatI WGTACW 2 cut(s) 367, 990
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 2 cut(s) 97, 269
Tru1I TTAA 4 cut(s) 188, 704, 740, 1074
Tru9I TTAA 4 cut(s) 188, 704, 740, 1074
TseI GCWGC 2 cut(s) 590, 1112
TspDTI ATGAA 7 cut(s) 172, 212, 486, 522, 617, 650, 797
TspGWI ACGGA 1 cut(s) 858
VpaK11BI GGWCC 1 cut(s) 49
XagI CCTNNNNNAGG 1 cut(s) 719
ZrmI AGTACT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.