Rh5BG144100

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000620
Physical Location & Seq
Reverse (-)
14954082 .. 14954966
885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG144100.1

Sequence Viewer

Length: 885 bp
ATGGTTCGAGCCAGTCCTCTGCAACTTTCCCATAAAAAAGAATGGGAACCTAACAAATTGATCTGGACCATTCTTTCTGAGGCAAACTTCAAGCTTATCTCAGGTGTGGCTGGCTTGCTTCTGGAGGAGAGTCTTACTTCTAGAGTTTCCCGAATCAGAAACTTTGCAACGCATCAAGTACTTTACTTGCCTGATGCACATGGGAAAACTAGTTCAGTGGGTTTTGTTTTTGATTCATCCACAGGTGAGTGTAAAGCGACATGTTTTCATTGGAAACAGGAGGGTGACACTGGCTATGAAGTAGGCTTTAAAGTTCTGAGTATCGGAAAGGATGATCAATGGAGGACCCTGAAGCTGCCCAAGCAAAATGGCAAATTGTTCGTGGCACATTATTTTACAGCAACTAACAAAGAAGAGGTCGCTCATGTGGTGGAAATTATTAGAGATGGACAAGATTTCAAGCTAGAAGTTCAATCTGTTGATATATGGAGTGAATGTTTCACCACTACCACTCTGCCCCGGGGAGCTTTCTTAGACTTGAAAAGAGTCACAAGTTTTCGTTGGAATTACTATGTAGCTGTTGCTGATATAGTAGAGGAATCCCTTAACGTCTTGGTGTTGGAAGACTTCAAGGAACACAAATGGAGGAAGATCATTGTTCCCTTGAAATTTTTGAAGGACAATCCAGGTTTAAAAGATGAGATTCGCCCTCATGCAGTTTGTTTAAATAACCTCCAGTTGTACAATGCAGTGAAAAAGAATATTCTCGTGTATGATATGGAAAGGAAGGTGATCAAGGTGACGCATACCAAGTCTACGGAGAAAATATATGAATTCCGAAAGCCAAGCCTGGTTACTCTCAAGGGAATGAAGAATGAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

294

Amino Acids

33.91

Weight (kDa)

9.05

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 30 - 274 1.4e-09 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 815
AcsI RAATTY 2 cut(s) 668, 833
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 2 cut(s) 180, 743
AflIII ACRYGT 1 cut(s) 260
AgsI TTSAA 7 cut(s) 91, 460, 473, 541, 631, 667, 676
AhlI ACTAGT 1 cut(s) 209
AjnI CCWGG 2 cut(s) 685, 849
AloI GAACNNNNNNTCC 2 cut(s) 196, 228
AluBI AGCT 5 cut(s) 94, 355, 463, 527, 578
AluI AGCT 5 cut(s) 94, 355, 463, 527, 578
Ama87I CYCGRG 1 cut(s) 519
ApeKI GCWGC 1 cut(s) 355
ApoI RAATTY 2 cut(s) 668, 833
ArsI GACNNNNNNTTYG 2 cut(s) 402, 434
AspS9I GGNCC 2 cut(s) 66, 345
AsuC2I CCSGG 2 cut(s) 520, 521
AsuHPI GGTGA 5 cut(s) 257, 296, 493, 802, 811
AvaI CYCGRG 1 cut(s) 519
AvaII GGWCC 2 cut(s) 66, 345
BarI GAAGNNNNNNTAC 1 cut(s) 863
BauI CACGAG 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 342
BccI CCATC 1 cut(s) 440
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BciT130I CCWGG 2 cut(s) 687, 851
BclI TGATCA 2 cut(s) 334, 792
BcnI CCSGG 2 cut(s) 520, 521
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 4 cut(s) 141, 210, 464, 883
BisI GCNGC 1 cut(s) 356
BlsI GCNGC 1 cut(s) 357
BmcAI AGTACT 1 cut(s) 180
Bme1390I CCNGG 4 cut(s) 520, 521, 687, 851
Bme18I GGWCC 2 cut(s) 66, 345
BmeT110I CYCGRG 1 cut(s) 519
BmgT120I GGNCC 2 cut(s) 66, 345
BmiI GGNNCC 2 cut(s) 48, 347
BmrFI CCNGG 4 cut(s) 520, 521, 687, 851
BmsI GCATC 2 cut(s) 181, 184
BpiI GAAGAC 1 cut(s) 630
BpmI CTGGAG 2 cut(s) 143, 719
BpuEI CTTGAG 1 cut(s) 845
BpuMI CCSGG 2 cut(s) 520, 521
BsaJI CCNNGG 3 cut(s) 518, 519, 520
BsaXI ACNNNNNCTCC 2 cut(s) 481, 511
Bse1I ACTGG 3 cut(s) 12, 295, 736
BseBI CCWGG 2 cut(s) 687, 851
BseDI CCNNGG 3 cut(s) 518, 519, 520
BseGI GGATG 2 cut(s) 236, 337
BseMII CTCAG 3 cut(s) 69, 114, 308
BseNI ACTGG 3 cut(s) 12, 295, 736
BseRI GAGGAG 1 cut(s) 140
BseXI GCAGC 1 cut(s) 342
BsiHKCI CYCGRG 1 cut(s) 519
BsiSI CCGG 1 cut(s) 520
BsoBI CYCGRG 1 cut(s) 519
Bsp1407I TGTACA 1 cut(s) 741
Bsp143I GATC 4 cut(s) 60, 334, 651, 792
BspCNI CTCAG 3 cut(s) 70, 113, 309
BspLI GGNNCC 2 cut(s) 48, 347
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 3 cut(s) 12, 295, 736
BssECI CCNNGG 3 cut(s) 518, 519, 520
BssMI GATC 4 cut(s) 60, 334, 651, 792
BssSI CACGAG 1 cut(s) 767
Bst2BI CACGAG 1 cut(s) 767
Bst2UI CCWGG 2 cut(s) 687, 851
Bst6I CTCTTC 1 cut(s) 408
BstAUI TGTACA 1 cut(s) 741
BstC8I GCNNGC 2 cut(s) 112, 116
BstDEI CTNAG 4 cut(s) 78, 100, 317, 532
BstF5I GGATG 2 cut(s) 236, 337
BstKTI GATC 4 cut(s) 63, 337, 654, 795
BstMBI GATC 4 cut(s) 60, 334, 651, 792
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstNI CCWGG 2 cut(s) 687, 851
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 4 cut(s) 518, 519, 685, 849
BstV1I GCAGC 1 cut(s) 342
BstV2I GAAGAC 1 cut(s) 630
BtsCI GGATG 2 cut(s) 236, 337
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 3 cut(s) 222, 288, 756
Cac8I GCNNGC 2 cut(s) 112, 116
Cfr13I GGNCC 2 cut(s) 66, 345
Cfr9I CCCGGG 1 cut(s) 519
CseI GACGC 1 cut(s) 811
Csp6I GTAC 2 cut(s) 179, 742
CviAII CATG 4 cut(s) 200, 261, 425, 713
CviQI GTAC 2 cut(s) 179, 742
DdeI CTNAG 4 cut(s) 78, 100, 317, 532
DpnI GATC 4 cut(s) 62, 336, 653, 794
DpnII GATC 4 cut(s) 60, 334, 651, 792
DraI TTTAAA 3 cut(s) 310, 693, 726
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco47I GGWCC 2 cut(s) 66, 345
Eco57I CTGAAG 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 345
EcoRI GAATTC 1 cut(s) 833
EcoRII CCWGG 2 cut(s) 685, 849
FaeI CATG 4 cut(s) 203, 264, 428, 716
FatI CATG 4 cut(s) 199, 260, 424, 712
FbaI TGATCA 2 cut(s) 334, 792
FblI GTMKAC 1 cut(s) 815
Fnu4HI GCNGC 1 cut(s) 356
FokI GGATG 2 cut(s) 223, 344
Fsp4HI GCNGC 1 cut(s) 356
FspBI CTAG 4 cut(s) 141, 210, 464, 883
GluI GCNGC 1 cut(s) 356
GsuI CTGGAG 2 cut(s) 143, 719
HapII CCGG 1 cut(s) 520
HgaI GACGC 1 cut(s) 811
Hin1II CATG 4 cut(s) 203, 264, 428, 716
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 6 cut(s) 130, 153, 233, 546, 599, 703
HpaII CCGG 1 cut(s) 520
HphI GGTGA 5 cut(s) 257, 296, 493, 802, 811
Hpy166II GTNNAC 1 cut(s) 816
Hpy188I TCNGA 5 cut(s) 79, 158, 318, 326, 839
Hpy188III TCNNGA 4 cut(s) 64, 122, 141, 150
Hpy8I GTNNAC 1 cut(s) 816
HpyAV CCTTC 2 cut(s) 670, 781
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 5 cut(s) 22, 167, 197, 716, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 4 cut(s) 78, 100, 317, 532
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 4 cut(s) 203, 264, 428, 716
Ksp22I TGATCA 2 cut(s) 334, 792
Kzo9I GATC 4 cut(s) 60, 334, 651, 792
LmnI GCTCC 1 cut(s) 524
Lsp1109I GCAGC 1 cut(s) 342
LweI GCATC 2 cut(s) 181, 184
MaeI CTAG 4 cut(s) 141, 210, 464, 883
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 4 cut(s) 284, 547, 799, 853
MalI GATC 4 cut(s) 62, 336, 653, 794
MboI GATC 4 cut(s) 60, 334, 651, 792
MboII GAAGA 4 cut(s) 425, 635, 661, 883
MluCI AATT 6 cut(s) 56, 374, 435, 565, 668, 833
MlyI GAGTC 2 cut(s) 139, 555
MmeI TCCRAC 2 cut(s) 542, 600
MseI TTAA 4 cut(s) 309, 606, 692, 725
MspI CCGG 1 cut(s) 520
MspR9I CCNGG 4 cut(s) 520, 521, 687, 851
MvaI CCWGG 2 cut(s) 687, 851
MwoI GCNNNNNNNGC 1 cut(s) 361
NciI CCSGG 2 cut(s) 520, 521
NdeII GATC 4 cut(s) 60, 334, 651, 792
NlaIII CATG 4 cut(s) 203, 264, 428, 716
NlaIV GGNNCC 2 cut(s) 48, 347
NmuCI GTSAC 3 cut(s) 284, 547, 799
NspI RCATGY 1 cut(s) 264
PciI ACATGT 1 cut(s) 260
PfeI GAWTC 4 cut(s) 153, 233, 599, 703
PkrI GCNGC 1 cut(s) 357
PleI GAGTC 2 cut(s) 138, 554
PpsI GAGTC 2 cut(s) 138, 554
PpuMI RGGWCCY 1 cut(s) 345
PscI ACATGT 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 345
Psp6I CCWGG 2 cut(s) 685, 849
PspGI CCWGG 2 cut(s) 685, 849
PspN4I GGNNCC 2 cut(s) 48, 347
PspPI GGNCC 2 cut(s) 66, 345
PspPPI RGGWCCY 1 cut(s) 345
RsaI GTAC 2 cut(s) 180, 743
RsaNI GTAC 2 cut(s) 179, 742
SaqAI TTAA 4 cut(s) 309, 606, 692, 725
SatI GCNGC 1 cut(s) 356
Sau3AI GATC 4 cut(s) 60, 334, 651, 792
Sau96I GGNCC 2 cut(s) 66, 345
ScaI AGTACT 1 cut(s) 180
SchI GAGTC 2 cut(s) 139, 555
ScrFI CCNGG 4 cut(s) 520, 521, 687, 851
SfaNI GCATC 2 cut(s) 181, 184
SinI GGWCC 2 cut(s) 66, 345
SmaI CCCGGG 1 cut(s) 521
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SpeI ACTAGT 1 cut(s) 209
Sse9I AATT 6 cut(s) 56, 374, 435, 565, 668, 833
SspI AATATT 1 cut(s) 763
SspMI CTAG 4 cut(s) 141, 210, 464, 883
StyD4I CCNGG 4 cut(s) 518, 519, 685, 849
TaiI ACGT 1 cut(s) 612
TaqI TCGA 1 cut(s) 7
TasI AATT 6 cut(s) 56, 374, 435, 565, 668, 833
TatI WGTACW 2 cut(s) 178, 741
TfiI GAWTC 4 cut(s) 153, 233, 599, 703
Tru1I TTAA 4 cut(s) 309, 606, 692, 725
Tru9I TTAA 4 cut(s) 309, 606, 692, 725
TscAI CASTG 3 cut(s) 222, 295, 756
TseFI GTSAC 3 cut(s) 284, 547, 799
TseI GCWGC 1 cut(s) 355
Tsp45I GTSAC 3 cut(s) 284, 547, 799
TspDTI ATGAA 5 cut(s) 225, 257, 312, 846, 884
TspGWI ACGGA 1 cut(s) 833
TspMI CCCGGG 1 cut(s) 519
TspRI CASTG 3 cut(s) 222, 295, 756
VpaK11BI GGWCC 2 cut(s) 66, 345
XapI RAATTY 2 cut(s) 668, 833
XbaI TCTAGA 1 cut(s) 140
XceI RCATGY 1 cut(s) 264
XmaI CCCGGG 1 cut(s) 519
XmiI GTMKAC 1 cut(s) 815
XspI CTAG 4 cut(s) 141, 210, 464, 883
ZrmI AGTACT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.