MD10G1311600.v1.1

A Receptor for Ubiquitination Targets

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000620
Physical Location & Seq
Forward (+)
39635799 .. 39637402
1604 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1311600.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGATGTGTTACTACCAACGAAGAAAAAGGCCGACACAAGTATCGTCAGAGCTTCCTTTTGAAATTATCAGTGAGATTTTGAGTTGGCTTCCTGTATTGTCTTTGTTGAAATTCAAGTGCGTGTGCAAGCAATGGTTTTTGTTGCTTCAAGACTACAAGTTCATTGCAAAGCATAGCAATCAGACCTGTTGCATCCTCTCACCTTACTATCATTGCGAATGGGAAAACAAATCGATTGTCACTGTTAGTGATAAAAAGTATGAGCTCAAAGGTTACTGTTCTGGCTTGTCTTTGGAGAAGAGCACAACATCTCAAGTTTGCCGCATTAGAAATCCTGCGACCCGCAAAGTACTCTACTTGCCAGATGCACACGATGAGGGTTCCAAAGAATTAATGGACCTCGGTTTGAATTCACTCACTGGTGAGTGTAAAGTGTTCCGTGTCTATTTCGATACGGCACAGGAACAGGTAGGTATTGAAGTGATAACAATTGGAAAGGATGAAATGTGGAGACCTTTGCACAAACAAAACAAGAATTGGCTGGGACGAGGCAAACCGGTGCTGAAACAATCTCGCTGTGGGGCAGATAAGGTTGAATGGGCTCTTCACTTACCCGAAATTATAACCGAGGGACGTAAGTTGTGCTTACAAATTCATTCTCTTGATCTGTGGAGTGAACGTCTCACCACTACTACTCTGCCTCAAGGAGCTTTCATAGATTTGGAGAATCTCTGGCCTTTTCTTTGGGATAATCGTCCAGCCGTTGCTGGTATAGTAGGGGGGGACCTTCACATCTTGGTGTTAGTAGACTTCAAGGAGCACAAATGGAGTCAAAACAAGATCATCTTTCCCTTGAAAAATTTAGAGGTCGACGACACAATTTTGACGGATAAAATTGCACTCATACAAGGTCGTTCAAATCAACTCGTGTTTCTAAACGGAGAACAACTTATTTCATATGACATTGAAAAAAAGACTGTTAAGGTTTTTGCCAATTTAGAATCCCTGAAGGAACGAATACCTCGACTTAAGTCAACCCTCGTTACTCTCAAGGAAATGACATCAGAAATTAAAGGAGCACAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

363

Amino Acids

41.78

Weight (kDa)

8.51

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 16 - 54 5.8e-08 F-box domain
F-box-like PF12937 16 - 51 3.3e-06 F-box-like
FBA_3 PF08268 66 - 293 2.3e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 623
AccI GTMKAC 2 cut(s) 807, 870
AciI CCGC 2 cut(s) 322, 343
AcsI RAATTY 4 cut(s) 110, 409, 651, 859
AcuI CTGAAG 1 cut(s) 1028
AfaI GTAC 1 cut(s) 351
AflII CTTAAG 1 cut(s) 1028
AgeI ACCGGT 1 cut(s) 556
AluBI AGCT 3 cut(s) 52, 265, 710
AluI AGCT 3 cut(s) 52, 265, 710
Alw21I GWGCWC 4 cut(s) 267, 305, 822, 1081
Alw26I GTCTC 2 cut(s) 505, 686
AoxI GGCC 2 cut(s) 29, 734
ApoI RAATTY 4 cut(s) 110, 409, 651, 859
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
AseI ATTAAT 1 cut(s) 392
AsiGI ACCGGT 1 cut(s) 556
AspS9I GGNCC 2 cut(s) 397, 784
AsuHPI GGTGA 3 cut(s) 192, 434, 676
AvaII GGWCC 2 cut(s) 397, 784
BaeI ACNNNNGTAYC 2 cut(s) 24, 57
BanII GRGCYC 2 cut(s) 267, 604
BauI CACGAG 1 cut(s) 926
Bbv12I GWGCWC 4 cut(s) 267, 305, 822, 1081
BceAI ACGGC 2 cut(s) 471, 746
BcoDI GTCTC 2 cut(s) 505, 686
BfaI CTAG 1 cut(s) 1087
BfrI CTTAAG 1 cut(s) 1028
BisI GCNGC 1 cut(s) 322
BlsI GCNGC 1 cut(s) 323
BmcAI AGTACT 1 cut(s) 351
Bme18I GGWCC 2 cut(s) 397, 784
BmgT120I GGNCC 2 cut(s) 397, 784
BmiI GGNNCC 2 cut(s) 382, 785
BmsI GCATC 2 cut(s) 201, 355
BoxI GACNNNNGTC 1 cut(s) 1030
BpuEI CTTGAG 3 cut(s) 297, 687, 1034
Bsa29I ATCGAT 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 2 cut(s) 400, 627
BsaWI WCCGGW 1 cut(s) 556
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 1 cut(s) 424
Bse3DI GCAATG 3 cut(s) 137, 162, 211
BseCI ATCGAT 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 400, 627
BseGI GGATG 2 cut(s) 192, 505
BseMI GCAATG 3 cut(s) 137, 162, 211
BseNI ACTGG 1 cut(s) 424
BseYI CCCAGC 1 cut(s) 541
BshFI GGCC 2 cut(s) 31, 736
BshTI ACCGGT 1 cut(s) 556
BshVI ATCGAT 1 cut(s) 233
BsiHKAI GWGCWC 4 cut(s) 267, 305, 822, 1081
BsiSI CCGG 1 cut(s) 557
BslFI GGGAC 3 cut(s) 558, 645, 797
BsmAI GTCTC 2 cut(s) 505, 686
BsmBI CGTCTC 1 cut(s) 686
BsmFI GGGAC 3 cut(s) 558, 645, 797
BsnI GGCC 2 cut(s) 31, 736
Bso31I GGTCTC 1 cut(s) 505
Bsp1286I GDGCHC 5 cut(s) 267, 305, 604, 822, 1081
Bsp143I GATC 2 cut(s) 664, 840
BspACI CCGC 2 cut(s) 322, 343
BspANI GGCC 2 cut(s) 31, 736
BspDI ATCGAT 1 cut(s) 233
BspLI GGNNCC 2 cut(s) 382, 785
BspQI GCTCTTC 2 cut(s) 293, 609
BspTI CTTAAG 1 cut(s) 1028
BspTNI GGTCTC 1 cut(s) 505
BsrDI GCAATG 3 cut(s) 137, 162, 211
BsrFI RCCGGY 1 cut(s) 556
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 2 cut(s) 400, 627
BssMI GATC 2 cut(s) 664, 840
BssSI CACGAG 1 cut(s) 926
Bst2BI CACGAG 1 cut(s) 926
Bst4CI ACNGT 3 cut(s) 244, 278, 979
Bst6I CTCTTC 2 cut(s) 293, 609
BstAFI CTTAAG 1 cut(s) 1028
BstC8I GCNNGC 1 cut(s) 128
BstF5I GGATG 2 cut(s) 192, 505
BstKTI GATC 2 cut(s) 667, 843
BstMAI GTCTC 2 cut(s) 505, 686
BstMBI GATC 2 cut(s) 664, 840
BstPAI GACNNNNGTC 1 cut(s) 1030
Bsu15I ATCGAT 1 cut(s) 233
BsuRI GGCC 2 cut(s) 31, 736
BsuTUI ATCGAT 1 cut(s) 233
BtsCI GGATG 2 cut(s) 192, 505
BtsIMutI CAGTG 3 cut(s) 76, 240, 417
Cac8I GCNNGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 397, 784
ClaI ATCGAT 1 cut(s) 233
Csp6I GTAC 1 cut(s) 350
CspAI ACCGGT 1 cut(s) 556
CviQI GTAC 1 cut(s) 350
DpnI GATC 2 cut(s) 666, 842
DpnII GATC 2 cut(s) 664, 840
Eam1104I CTCTTC 2 cut(s) 293, 609
EarI CTCTTC 2 cut(s) 293, 609
Ecl136II GAGCTC 1 cut(s) 265
Eco24I GRGCYC 2 cut(s) 267, 604
Eco31I GGTCTC 1 cut(s) 505
Eco47I GGWCC 2 cut(s) 397, 784
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 1028
EcoICRI GAGCTC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 784
EcoRI GAATTC 1 cut(s) 409
EcoT38I GRGCYC 2 cut(s) 267, 604
Esp3I CGTCTC 1 cut(s) 686
FaiI YATR 8 cut(s) 174, 261, 623, 716, 773, 905, 958, 960
FalI AAGNNNNNCTT 4 cut(s) 629, 661, 830, 862
FaqI GGGAC 3 cut(s) 558, 645, 797
FauI CCCGC 1 cut(s) 350
FauNDI CATATG 1 cut(s) 958
FblI GTMKAC 2 cut(s) 807, 870
Fnu4HI GCNGC 1 cut(s) 322
FokI GGATG 2 cut(s) 179, 512
FriOI GRGCYC 2 cut(s) 267, 604
Fsp4HI GCNGC 1 cut(s) 322
FspBI CTAG 1 cut(s) 1087
GluI GCNGC 1 cut(s) 322
GsaI CCCAGC 1 cut(s) 545
HaeIII GGCC 2 cut(s) 31, 736
HapII CCGG 1 cut(s) 557
HincII GTYRAC 2 cut(s) 871, 1035
HindII GTYRAC 2 cut(s) 871, 1035
HinfI GANTC 3 cut(s) 727, 829, 1001
HpaII CCGG 1 cut(s) 557
HphI GGTGA 3 cut(s) 192, 434, 676
Hpy166II GTNNAC 4 cut(s) 677, 808, 871, 1035
Hpy188I TCNGA 3 cut(s) 49, 183, 1066
Hpy188III TCNNGA 2 cut(s) 149, 662
Hpy8I GTNNAC 4 cut(s) 677, 808, 871, 1035
Hpy99I CGWCG 1 cut(s) 875
HpyAV CCTTC 2 cut(s) 797, 1003
HpyCH4III ACNGT 3 cut(s) 244, 278, 979
HpyCH4IV ACGT 2 cut(s) 634, 679
HpyCH4V TGCA 6 cut(s) 126, 167, 192, 368, 520, 899
HpySE526I ACGT 2 cut(s) 634, 679
Kzo9I GATC 2 cut(s) 664, 840
LguI GCTCTTC 2 cut(s) 293, 609
LmnI GCTCC 3 cut(s) 707, 817, 1076
LweI GCATC 2 cut(s) 201, 355
MaeI CTAG 1 cut(s) 1087
MaeII ACGT 2 cut(s) 634, 679
MaeIII GTNAC 4 cut(s) 8, 238, 272, 1042
MalI GATC 2 cut(s) 666, 842
MboI GATC 2 cut(s) 664, 840
MboII GAAGA 3 cut(s) 33, 310, 596
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 5 cut(s) 267, 305, 604, 822, 1081
MlyI GAGTC 1 cut(s) 838
MnlI CCTC 9 cut(s) 206, 370, 410, 542, 622, 711, 859, 1032, 1049
MseI TTAA 4 cut(s) 392, 981, 1029, 1071
MslI CAYNNNNRTG 1 cut(s) 797
MspCI CTTAAG 1 cut(s) 1028
MspI CCGG 1 cut(s) 557
MunI CAATTG 1 cut(s) 489
NdeI CATATG 1 cut(s) 958
NdeII GATC 2 cut(s) 664, 840
NlaIV GGNNCC 2 cut(s) 382, 785
NmuCI GTSAC 1 cut(s) 238
PciSI GCTCTTC 2 cut(s) 293, 609
PcsI WCGNNNNNNNCGW 1 cut(s) 1021
PfeI GAWTC 2 cut(s) 727, 1001
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 1 cut(s) 323
PleI GAGTC 1 cut(s) 837
PpsI GAGTC 1 cut(s) 837
PpuMI RGGWCCY 1 cut(s) 784
PshAI GACNNNNGTC 1 cut(s) 1030
PshBI ATTAAT 1 cut(s) 392
PsiI TTATAA 1 cut(s) 623
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 784
PspFI CCCAGC 1 cut(s) 541
PspN4I GGNNCC 2 cut(s) 382, 785
PspPI GGNCC 2 cut(s) 397, 784
PspPPI RGGWCCY 1 cut(s) 784
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
RseI CAYNNNNRTG 1 cut(s) 797
SacI GAGCTC 1 cut(s) 267
SalI GTCGAC 1 cut(s) 869
SapI GCTCTTC 2 cut(s) 293, 609
SaqAI TTAA 4 cut(s) 392, 981, 1029, 1071
SatI GCNGC 1 cut(s) 322
Sau3AI GATC 2 cut(s) 664, 840
Sau96I GGNCC 2 cut(s) 397, 784
ScaI AGTACT 1 cut(s) 351
SchI GAGTC 1 cut(s) 838
SduI GDGCHC 5 cut(s) 267, 305, 604, 822, 1081
SfaNI GCATC 2 cut(s) 201, 355
SinI GGWCC 2 cut(s) 397, 784
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 4 cut(s) 312, 702, 1028, 1049
SmoI CTYRAG 4 cut(s) 312, 702, 1028, 1049
SsiI CCGC 2 cut(s) 322, 343
SspMI CTAG 1 cut(s) 1087
SstI GAGCTC 1 cut(s) 267
TaaI ACNGT 3 cut(s) 244, 278, 979
TaiI ACGT 2 cut(s) 637, 682
TaqI TCGA 4 cut(s) 233, 450, 870, 1024
TatI WGTACW 1 cut(s) 349
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 2 cut(s) 727, 1001
Tru1I TTAA 4 cut(s) 392, 981, 1029, 1071
Tru9I TTAA 4 cut(s) 392, 981, 1029, 1071
TscAI CASTG 3 cut(s) 76, 247, 424
TseFI GTSAC 1 cut(s) 238
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 5 cut(s) 151, 516, 644, 703, 945
TspGWI ACGGA 3 cut(s) 428, 902, 954
TspRI CASTG 3 cut(s) 76, 247, 424
Vha464I CTTAAG 1 cut(s) 1028
VpaK11BI GGWCC 2 cut(s) 397, 784
VspI ATTAAT 1 cut(s) 392
XapI RAATTY 4 cut(s) 110, 409, 651, 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmiI GTMKAC 2 cut(s) 807, 870
XspI CTAG 1 cut(s) 1087
ZrmI AGTACT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.