Rh5BG147400

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000620
Physical Location & Seq
Forward (+)
15150685 .. 15154024
3340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG147400.1

Sequence Viewer

Length: 1143 bp
ATGCCATCCACAGAGGACTGCTGCAAGTTACCTGCAAAGGATCGCCATGAGTTCTTTTCTCGCAGGGTTCGCATTGACAATCTAAATCTTAGCCAAAACCCGGCGATGTCTGGAGTGGTACCAGAGCTTCCCTTTGATATCATCACTGAGATTTTGAGTTGGTTGCCGGTAGAGTCTTTGCTCAGGTTCAAGTGTGTGTGCAAACAATGGTGTTCCTTGCTTCTAGACTACAAGTTCATTGCAAAACATATGCTTCGAGCCCGACCTCTGCAACTTTCCGATCAACGTATGTGGAAACATAACAAAAAGAGTACCTTTATTTATGATGAAAATGTCAAACGTGTTACAGAAGTGGCTGGCTTGTTTCTGGAGGAGAGTCTTACTACTCGAGTTTTCCGAATCAGAAACTTTGCAACGCACCAAGTCCTCTACTTGCCCTATGCAGGTAATGCTACTACCGCAATGGGTTTTGTTTTTGATTCATCCACTGGTGAGTGTAAAGTGGCATGTTTTTATAAGAATGAGGCGGGTGACTTCGGCCGTGAAGTAGGCGTAGAAGTTCTAAGTATCGGAAAGGATGATCAATGGAGGACCCTGAAGCTGCCCAACCAAAATGGCAAATTGTTCCGGAAAAGATATTTTAGAGCAACTCACGAAGAAGGGGCCGCTCATTTGGTGGAAATCATTAGAGATGGAGAAGATTTCAAGCTAGAAGTTCAATCTTTTGATCTGTGGAGTGAATGTTTCACCATTACCACTCTGCCCCGCGGAGCTTTCTTAGACTTGAAAAGAGTTAGAGTTTTTCGTTGGAATCAATATGTAGCTGTTGCTGATATAGTAGGGGAAGCCCTTAACGTCTTGGTGTTACAAGACTTCAAGAAGCACAAATGGAGTAAGATCACTGTTCCCCTGAAGTTTTTGAAGGACGACCCAGTTTTGAAAGATCAAATTAAACCTCGTGGAGCTAATAGGTTTGGTGAACTCCAGTTACACAATGCAATGAGAAATAATCTTCTTTTATATGATATGAAACGGGAGATGATTCAAGCCACCATTAACATTACAAACTCCTCAGAAAAAATATTCACTTTCCATAAGCCAAGCCTGGTTACTCTTAAGGGAATGAAGAGGGAATATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

380

Amino Acids

44.21

Weight (kDa)

9.3

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 42 - 79 5.1e-10 F-box domain
F-box-like PF12937 42 - 76 1.2e-07 F-box-like
FBA_3 PF08268 101 - 351 3.3e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
Acc36I ACCTGC 2 cut(s) 40, 434
Acc65I GGTACC 1 cut(s) 118
AccB1I GGYRCC 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 668
AccII CGCG 1 cut(s) 768
AccIII TCCGGA 1 cut(s) 627
AciI CCGC 5 cut(s) 459, 527, 666, 766, 768
AclWI GGATC 1 cut(s) 48
AcoI YGGCCR 1 cut(s) 538
AcuI CTGAAG 2 cut(s) 617, 932
AfaI GTAC 2 cut(s) 120, 313
AfiI CCNNNNNNNGG 2 cut(s) 100, 443
AflII CTTAAG 1 cut(s) 1115
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 8 cut(s) 190, 706, 719, 787, 877, 922, 940, 1046
AjnI CCWGG 1 cut(s) 1104
AluBI AGCT 6 cut(s) 127, 601, 709, 773, 824, 965
AluI AGCT 6 cut(s) 127, 601, 709, 773, 824, 965
AlwI GGATC 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 387
Aor13HI TCCGGA 1 cut(s) 627
AoxI GGCC 2 cut(s) 538, 663
ApeKI GCWGC 2 cut(s) 21, 601
Asp718I GGTACC 1 cut(s) 118
AspS9I GGNCC 2 cut(s) 591, 663
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 4 cut(s) 503, 542, 739, 989
AvaI CYCGRG 1 cut(s) 387
AvaII GGWCC 1 cut(s) 591
BanI GGYRCC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 262
BarI GAAGNNNNNNTAC 2 cut(s) 111, 143
BauI CACGAG 1 cut(s) 957
BbvI GCAGC 2 cut(s) 8, 588
BccI CCATC 2 cut(s) 13, 686
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 1 cut(s) 1106
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 101
BfaI CTAG 2 cut(s) 224, 710
BfrI CTTAAG 1 cut(s) 1115
BfuAI ACCTGC 2 cut(s) 40, 434
BisI GCNGC 3 cut(s) 22, 602, 666
BlsI GCNGC 3 cut(s) 23, 603, 667
Bme1390I CCNGG 2 cut(s) 101, 1106
Bme18I GGWCC 1 cut(s) 591
BmeT110I CYCGRG 1 cut(s) 387
BmgT120I GGNCC 2 cut(s) 591, 663
BmiI GGNNCC 3 cut(s) 120, 593, 664
BmrFI CCNGG 2 cut(s) 101, 1106
BmrI ACTGGG 1 cut(s) 926
BmuI ACTGGG 1 cut(s) 926
BpmI CTGGAG 3 cut(s) 132, 389, 968
Bpu10I CCTNAGC 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 101
BsaJI CCNNGG 1 cut(s) 766
BsaWI WCCGGW 1 cut(s) 627
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 443
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 3 cut(s) 493, 932, 985
Bse3DI GCAATG 3 cut(s) 237, 468, 1005
BseAI TCCGGA 1 cut(s) 627
BseBI CCWGG 1 cut(s) 1106
BseDI CCNNGG 1 cut(s) 766
BseGI GGATG 3 cut(s) 5, 482, 583
BseLI CCNNNNNNNGG 2 cut(s) 100, 443
BseMI GCAATG 3 cut(s) 237, 468, 1005
BseMII CTCAG 3 cut(s) 138, 196, 1086
BseNI ACTGG 3 cut(s) 493, 932, 985
BseRI GAGGAG 2 cut(s) 386, 1060
BseX3I CGGCCG 1 cut(s) 538
BseXI GCAGC 2 cut(s) 8, 588
Bsh1236I CGCG 1 cut(s) 768
Bsh1285I CGRYCG 1 cut(s) 541
BshFI GGCC 2 cut(s) 540, 665
BshNI GGYRCC 1 cut(s) 118
BsiEI CGRYCG 1 cut(s) 541
BsiHKCI CYCGRG 1 cut(s) 387
BsiSI CCGG 3 cut(s) 101, 167, 628
BslI CCNNNNNNNGG 2 cut(s) 100, 443
BsnI GGCC 2 cut(s) 540, 665
BsoBI CYCGRG 1 cut(s) 387
Bsp1286I GDGCHC 1 cut(s) 262
Bsp13I TCCGGA 1 cut(s) 627
Bsp143I GATC 6 cut(s) 40, 280, 580, 727, 897, 943
BspACI CCGC 5 cut(s) 459, 527, 666, 766, 768
BspANI GGCC 2 cut(s) 540, 665
BspCNI CTCAG 3 cut(s) 139, 195, 1085
BspEI TCCGGA 1 cut(s) 627
BspFNI CGCG 1 cut(s) 768
BspLI GGNNCC 3 cut(s) 120, 593, 664
BspMI ACCTGC 2 cut(s) 40, 434
BspPI GGATC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 118
BspTI CTTAAG 1 cut(s) 1115
BsrBI CCGCTC 1 cut(s) 668
BsrDI GCAATG 3 cut(s) 237, 468, 1005
BsrFI RCCGGY 1 cut(s) 166
BsrI ACTGG 3 cut(s) 493, 932, 985
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 766
BssMI GATC 6 cut(s) 40, 280, 580, 727, 897, 943
BssSI CACGAG 1 cut(s) 957
Bst2BI CACGAG 1 cut(s) 957
Bst2UI CCWGG 1 cut(s) 1106
Bst4CI ACNGT 1 cut(s) 904
Bst6I CTCTTC 1 cut(s) 1121
BstAFI CTTAAG 1 cut(s) 1115
BstAPI GCANNNNNTGC 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 6 cut(s) 89, 147, 182, 563, 778, 1072
BstDSI CCRYGG 1 cut(s) 766
BstF5I GGATG 3 cut(s) 5, 482, 583
BstFNI CGCG 1 cut(s) 768
BstKTI GATC 6 cut(s) 43, 283, 583, 730, 900, 946
BstMBI GATC 6 cut(s) 40, 280, 580, 727, 897, 943
BstMCI CGRYCG 1 cut(s) 541
BstMWI GCNNNNNNNGC 3 cut(s) 69, 449, 458
BstNI CCWGG 1 cut(s) 1106
BstNSI RCATGY 1 cut(s) 510
BstSCI CCNGG 2 cut(s) 99, 1104
BstUI CGCG 1 cut(s) 768
BstV1I GCAGC 2 cut(s) 8, 588
BstZI CGGCCG 1 cut(s) 538
BsuRI GGCC 2 cut(s) 540, 665
BtgI CCRYGG 1 cut(s) 766
BtgZI GCGATG 1 cut(s) 119
BtsCI GGATG 3 cut(s) 5, 482, 583
BtsIMutI CAGTG 3 cut(s) 144, 486, 900
BveI ACCTGC 2 cut(s) 40, 434
Cac8I GCNNGC 1 cut(s) 358
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 591, 663
Cfr42I CCGCGG 1 cut(s) 769
Csp6I GTAC 2 cut(s) 119, 312
CspCI CAANNNNNGTGG 2 cut(s) 272, 307
CviAII CATG 2 cut(s) 47, 507
CviQI GTAC 2 cut(s) 119, 312
DdeI CTNAG 6 cut(s) 89, 147, 182, 563, 778, 1072
DpnI GATC 6 cut(s) 42, 282, 582, 729, 899, 945
DpnII GATC 6 cut(s) 40, 280, 580, 727, 897, 943
EaeI YGGCCR 1 cut(s) 538
EagI CGGCCG 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 1121
EarI CTCTTC 1 cut(s) 1121
EclXI CGGCCG 1 cut(s) 538
Eco24I GRGCYC 1 cut(s) 262
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 591
Eco52I CGGCCG 1 cut(s) 538
Eco57I CTGAAG 2 cut(s) 617, 932
Eco88I CYCGRG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 1104
EcoRV GATATC 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 262
FaeI CATG 2 cut(s) 50, 510
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FatI CATG 2 cut(s) 46, 506
FauI CCCGC 2 cut(s) 520, 773
FauNDI CATATG 1 cut(s) 249
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 3 cut(s) 22, 602, 666
FokI GGATG 2 cut(s) 469, 590
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 3 cut(s) 22, 602, 666
FspBI CTAG 2 cut(s) 224, 710
GluI GCNGC 3 cut(s) 22, 602, 666
GsuI CTGGAG 3 cut(s) 132, 389, 968
HaeIII GGCC 2 cut(s) 540, 665
HapII CCGG 3 cut(s) 101, 167, 628
Hin1II CATG 2 cut(s) 50, 510
HinfI GANTC 6 cut(s) 173, 376, 399, 479, 811, 1042
HpaII CCGG 3 cut(s) 101, 167, 628
HphI GGTGA 4 cut(s) 503, 542, 739, 989
Hpy166II GTNNAC 1 cut(s) 980
Hpy188I TCNGA 5 cut(s) 280, 398, 404, 572, 1075
Hpy188III TCNNGA 6 cut(s) 111, 224, 368, 628, 653, 877
Hpy8I GTNNAC 1 cut(s) 980
HpyAV CCTTC 2 cut(s) 653, 916
HpyCH4III ACNGT 1 cut(s) 904
HpyCH4IV ACGT 3 cut(s) 286, 340, 855
HpyCH4V TGCA 8 cut(s) 24, 35, 201, 242, 271, 413, 443, 998
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 449, 458
HpyF3I CTNAG 6 cut(s) 89, 147, 182, 563, 778, 1072
HpySE526I ACGT 3 cut(s) 286, 340, 855
Hsp92II CATG 2 cut(s) 50, 510
Kpn2I TCCGGA 1 cut(s) 627
KpnI GGTACC 1 cut(s) 122
Ksp22I TGATCA 1 cut(s) 580
KspI CCGCGG 1 cut(s) 769
Kzo9I GATC 6 cut(s) 40, 280, 580, 727, 897, 943
LmnI GCTCC 2 cut(s) 770, 962
Lsp1109I GCAGC 2 cut(s) 8, 588
MaeI CTAG 2 cut(s) 224, 710
MaeII ACGT 3 cut(s) 286, 340, 855
MaeIII GTNAC 6 cut(s) 27, 343, 530, 864, 987, 1108
MalI GATC 6 cut(s) 42, 282, 582, 729, 899, 945
MbiI CCGCTC 1 cut(s) 668
MboI GATC 6 cut(s) 40, 280, 580, 727, 897, 943
MboII GAAGA 4 cut(s) 668, 710, 1004, 1138
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 2 cut(s) 620, 948
MlyI GAGTC 2 cut(s) 182, 385
MmeI TCCRAC 1 cut(s) 788
MnlI CCTC 9 cut(s) 7, 276, 364, 437, 517, 582, 966, 1081, 1122
MroI TCCGGA 1 cut(s) 627
MseI TTAA 4 cut(s) 852, 951, 1056, 1116
MspA1I CMGCKG 1 cut(s) 768
MspCI CTTAAG 1 cut(s) 1115
MspI CCGG 3 cut(s) 101, 167, 628
MspR9I CCNGG 2 cut(s) 101, 1106
MvaI CCWGG 1 cut(s) 1106
MvnI CGCG 1 cut(s) 768
MwoI GCNNNNNNNGC 3 cut(s) 69, 449, 458
NciI CCSGG 1 cut(s) 101
NdeI CATATG 1 cut(s) 249
NdeII GATC 6 cut(s) 40, 280, 580, 727, 897, 943
NlaIII CATG 2 cut(s) 50, 510
NlaIV GGNNCC 3 cut(s) 120, 593, 664
NmuCI GTSAC 1 cut(s) 530
NspI RCATGY 1 cut(s) 510
PaeR7I CTCGAG 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 394
PfeI GAWTC 4 cut(s) 399, 479, 811, 1042
PkrI GCNGC 3 cut(s) 23, 603, 667
PleI GAGTC 2 cut(s) 181, 384
PpsI GAGTC 2 cut(s) 181, 384
PpuMI RGGWCCY 1 cut(s) 591
PsiI TTATAA 1 cut(s) 516
Psp5II RGGWCCY 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 1104
PspGI CCWGG 1 cut(s) 1104
PspN4I GGNNCC 3 cut(s) 120, 593, 664
PspPI GGNCC 2 cut(s) 591, 663
PspPPI RGGWCCY 1 cut(s) 591
PspXI VCTCGAGB 1 cut(s) 387
PsrI GAACNNNNNNTAC 2 cut(s) 972, 1004
RsaI GTAC 2 cut(s) 120, 313
RsaNI GTAC 2 cut(s) 119, 312
SacII CCGCGG 1 cut(s) 769
SaqAI TTAA 4 cut(s) 852, 951, 1056, 1116
SatI GCNGC 3 cut(s) 22, 602, 666
Sau3AI GATC 6 cut(s) 40, 280, 580, 727, 897, 943
Sau96I GGNCC 2 cut(s) 591, 663
SchI GAGTC 2 cut(s) 182, 385
ScrFI CCNGG 2 cut(s) 101, 1106
SduI GDGCHC 1 cut(s) 262
Sfr274I CTCGAG 1 cut(s) 387
Sfr303I CCGCGG 1 cut(s) 769
SgrBI CCGCGG 1 cut(s) 769
SinI GGWCC 1 cut(s) 591
SlaI CTCGAG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 387, 1115
SmoI CTYRAG 2 cut(s) 387, 1115
Sse9I AATT 2 cut(s) 620, 948
SsiI CCGC 5 cut(s) 459, 527, 666, 766, 768
SspI AATATT 1 cut(s) 1083
SspMI CTAG 2 cut(s) 224, 710
StyD4I CCNGG 2 cut(s) 99, 1104
TaaI ACNGT 1 cut(s) 904
TaiI ACGT 3 cut(s) 289, 343, 858
TaqI TCGA 2 cut(s) 256, 388
TasI AATT 2 cut(s) 620, 948
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 4 cut(s) 399, 479, 811, 1042
Tru1I TTAA 4 cut(s) 852, 951, 1056, 1116
Tru9I TTAA 4 cut(s) 852, 951, 1056, 1116
TscAI CASTG 3 cut(s) 151, 493, 907
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 2 cut(s) 21, 601
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 5 cut(s) 226, 342, 471, 1043, 1139
TspRI CASTG 3 cut(s) 151, 493, 907
Vha464I CTTAAG 1 cut(s) 1115
VpaK11BI GGWCC 1 cut(s) 591
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 1 cut(s) 510
XhoI CTCGAG 1 cut(s) 387
XspI CTAG 2 cut(s) 224, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.