MD05G1336100.v1.1

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000620
Physical Location & Seq
Forward (+)
45842901 .. 45844861
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1336100.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGGGAGTCCAATGTGTCGATACCAAAGAAGAAGAAGAAGTGCCGCGGCAACAATTACGAGAGCTTCCCTTTGATATTGTTGCTCAGATTTTGTTGTGGCTGCCCGTGGTGTCTTTGGTGAGATTCAAGTGTGTCTGCAAGCAATGGTGTCTGTTAATTGAGGGCTACAAGTTCATAGAAAAGCATAGGGCTCTAACACATTACGGTTTGCCACCTTGTTATCAATGCCAATGGGATAAGGAGAGAATGAAGGGAGTTGCTTCCTGTGGGGATTTCGAGCTTCAATGTGATTGTAGTGGCTTGTATTTGGAGAAGAGTAAAACTTCTCAAGTTTGCCGTATTAGAAATCCTGCAACCCACAAAGTGCTCTACTTGCCCAAGGCACCCGAGGGTACCAGAGTAGTTCTGGACCTCGGTTTTAATTCCCTTACTGGTGAGTGTAAAGTGGCCTGTGCTTATCGCCGAGAGGGCAGTTTAGTAGGTGTTGATGTTATAACAATTGGAAAGGATGAAATGTGGAGACCCTTGCTCCGCCAAAAGCTGAATTTTCTGGAACAAGGCAGATGGGTGGTGAAGCCAAATTGTTGTGAGGAAGATAAGGTTAACTGGGCTATTTATATCTCCAAAATTATAAAAGATGAACACAATGATGATTCGTGCCTACAAATTCAGTCTCTTGATCTGTGGAGTGAATGTCTCACCACAACTACTTTGCCCCAGGGAGTTTTCCTAAATTTGAAGAATGTTCGGGCTTTTCCTTGGGATCTCTGTCTAGGCGTTGCTGAAACAGTAGGGGGAGCCATTCACATCTTGGTGTTAGAAGACTTCAAGGAACACAAATGGAGTCGAAACAAGATCATCATTCCTTTGAAAAATTTAAAGGTTGACTACACAGTTTGGACGGATCATTTAGTTCTCACACAAGCTCATTCTAGCTCACTCAGGTTTCGAAATGGCCAAGGGCAAGTTCTTTCCTATGACATGGAAACTGAGGACCTTGAGGTTTTGGAACGTTCGGAATTCCTCAAGAAACGACTTGCTCTACGCAAATCAACCCTCGTGACTGTAAAGGGAATGAGACTAGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

364

Amino Acids

41.84

Weight (kDa)

7.45

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 21 - 58 2.2e-08 F-box domain
FBA_3 PF08268 99 - 341 2.3e-12 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000620)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03910 FvH4_4g13750 FvH4_6g21492 FvH4_6g21740 FvH4_6g24450
malus_domestica MD05G1336000.v1.1 MD05G1336100.v1.1 MD10G1212400.v1.1 MD10G1311600.v1.1
prunus_persica Prupe.4G112200_v2.0.a1 Prupe.4G130000_v2.0.a1 Prupe.4G130300_v2.0.a1 Prupe.4G219800_v2.0.a1 Prupe.4G219900_v2.0.a1 Prupe.4G220100_v2.0.a1
pyrus_communis pycom05g30760 pycom10g26280
rosa_chinensis RchiOBHm_Chr3g0478691 RchiOBHm_Chr3g0479021 RchiOBHm_Chr5g0004511 RchiOBHm_Chr5g0019881 RchiOBHm_Chr5g0021181
rosa_laevigata RLG00000023610 RLG00000023612 RLG00000031235 RLG00000032481 RLG00000032506 RLG00000032589 RLG00000032592
rosa_multiflora Rmu_co8446543.1_g000001 Rmu_sc0001396.1_g000010 Rmu_sc0002454.1_g000005 Rmu_sc0002531.1_g000072 Rmu_sc0002715.1_g000028 Rmu_sc0005207.1_g000008 Rmu_sc0005645.1_g000010 Rmu_sc0008074.1_g000017 Rmu_sc0012286.1_g000001 Rmu_ssc0000244.1_g000041
rosa_roxburghii Rroxscaffold_1G00039650 Rroxscaffold_1G00058030 Rroxscaffold_1G00058290 Rroxscaffold_1G00071010 Rroxscaffold_6G00402820 Rroxscaffold_6G00403180
rosa_rugosa Rorug03G0167000 Rorug03G0167100 Rorug03G0170200 Rorug03G0304300 Rorug05G0054800 Rorug05G0065000 Rorug05G0189900
rosa_samantha Rh3AG219300 Rh3AG221100 Rh3BG251000 Rh3BG253400 Rh3BG253800 Rh3CG247800 Rh3CG250000 Rh3DG244100 Rh3DG245900 Rh4AG008500 Rh4CG009200 Rh4CG009600 Rh5AG275400 Rh5BG144100 Rh5BG144400 Rh5BG144500 Rh5BG147400 Rh5BG280200 Rh5CG043100 Rh5CG155800 Rh5CG312400 Rh5DG143800 Rh5DG146700 Rh5DG288400
rosa_wichuraiana Rw3G019900 Rw5G012800 Rw5G013070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 494, 632
Acc65I GGTACC 1 cut(s) 392
AccB1I GGYRCC 2 cut(s) 382, 392
AccII CGCG 1 cut(s) 46
AciI CCGC 3 cut(s) 44, 46, 532
AclI AACGTT 1 cut(s) 1012
AclWI GGATC 2 cut(s) 771, 912
AcoI YGGCCR 1 cut(s) 955
AcsI RAATTY 5 cut(s) 544, 666, 733, 874, 1019
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 1 cut(s) 394
AgsI TTSAA 5 cut(s) 127, 284, 739, 829, 871
AjnI CCWGG 1 cut(s) 717
AjuI GAANNNNNNNTTGG 2 cut(s) 951, 983
AluBI AGCT 5 cut(s) 64, 280, 541, 926, 936
AluI AGCT 5 cut(s) 64, 280, 541, 926, 936
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 4 cut(s) 514, 678, 701, 1072
AlwI GGATC 2 cut(s) 771, 912
Ama87I CYCGRG 1 cut(s) 386
AoxI GGCC 2 cut(s) 447, 955
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 5 cut(s) 544, 666, 733, 874, 1019
Asp718I GGTACC 1 cut(s) 392
AspS9I GGNCC 2 cut(s) 409, 994
AsuHPI GGTGA 4 cut(s) 130, 446, 583, 691
AsuII TTCGAA 1 cut(s) 949
AvaI CYCGRG 1 cut(s) 386
AvaII GGWCC 2 cut(s) 409, 994
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BalI TGGCCA 1 cut(s) 957
BanI GGYRCC 2 cut(s) 382, 392
BanII GRGCYC 1 cut(s) 193
BauI CACGAG 1 cut(s) 1058
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 87
BccI CCATC 1 cut(s) 558
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 1 cut(s) 719
BcoDI GTCTC 4 cut(s) 514, 678, 701, 1072
BfaI CTAG 3 cut(s) 773, 933, 1082
BglI GCCNNNNNGGC 1 cut(s) 468
BisI GCNGC 3 cut(s) 44, 47, 101
BlsI GCNGC 3 cut(s) 45, 48, 102
Bme1390I CCNGG 1 cut(s) 719
Bme18I GGWCC 2 cut(s) 409, 994
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 2 cut(s) 409, 994
BmiI GGNNCC 3 cut(s) 384, 394, 799
BmrFI CCNGG 1 cut(s) 719
BmrI ACTGGG 1 cut(s) 616
BmuI ACTGGG 1 cut(s) 616
BpiI GAAGAC 1 cut(s) 828
Bpu14I TTCGAA 1 cut(s) 949
BpuEI CTTGAG 3 cut(s) 312, 1010, 1019
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 9 cut(s) 44, 105, 378, 387, 412, 717, 718, 758, 958
BsaXI ACNNNNNCTCC 4 cut(s) 513, 543, 679, 709
Bse1I ACTGG 2 cut(s) 436, 611
Bse3DI GCAATG 1 cut(s) 149
BseBI CCWGG 1 cut(s) 719
BseDI CCNNGG 9 cut(s) 44, 105, 378, 387, 412, 717, 718, 758, 958
BseGI GGATG 1 cut(s) 514
BseMI GCAATG 1 cut(s) 149
BseMII CTCAG 3 cut(s) 98, 955, 981
BseNI ACTGG 2 cut(s) 436, 611
BseXI GCAGC 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 46
BshFI GGCC 2 cut(s) 449, 957
BshNI GGYRCC 2 cut(s) 382, 392
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 386
BsmAI GTCTC 4 cut(s) 514, 678, 701, 1072
BsnI GGCC 2 cut(s) 449, 957
Bso31I GGTCTC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 949
Bsp1286I GDGCHC 2 cut(s) 193, 369
Bsp143I GATC 4 cut(s) 679, 763, 855, 904
BspACI CCGC 3 cut(s) 44, 46, 532
BspANI GGCC 2 cut(s) 449, 957
BspCNI CTCAG 3 cut(s) 97, 954, 982
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 384, 394, 799
BspPI GGATC 2 cut(s) 771, 912
BspT104I TTCGAA 1 cut(s) 949
BspT107I GGYRCC 2 cut(s) 382, 392
BspTNI GGTCTC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 149
BsrI ACTGG 2 cut(s) 436, 611
BssECI CCNNGG 9 cut(s) 44, 105, 378, 387, 412, 717, 718, 758, 958
BssMI GATC 4 cut(s) 679, 763, 855, 904
BssSI CACGAG 1 cut(s) 1058
BssT1I CCWWGG 3 cut(s) 378, 758, 958
Bst2BI CACGAG 1 cut(s) 1058
Bst2UI CCWGG 1 cut(s) 719
Bst4CI ACNGT 4 cut(s) 206, 790, 895, 1066
Bst6I CTCTTC 1 cut(s) 308
BstBI TTCGAA 1 cut(s) 949
BstC8I GCNNGC 1 cut(s) 140
BstDEI CTNAG 3 cut(s) 84, 941, 990
BstDSI CCRYGG 2 cut(s) 44, 105
BstF5I GGATG 1 cut(s) 514
BstFNI CGCG 1 cut(s) 46
BstKTI GATC 4 cut(s) 682, 766, 858, 907
BstMAI GTCTC 4 cut(s) 514, 678, 701, 1072
BstMBI GATC 4 cut(s) 679, 763, 855, 904
BstMWI GCNNNNNNNGC 2 cut(s) 373, 468
BstNI CCWGG 1 cut(s) 719
BstSCI CCNGG 1 cut(s) 717
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 1 cut(s) 87
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 1 cut(s) 763
BstYI RGATCY 1 cut(s) 763
BsuRI GGCC 2 cut(s) 449, 957
BtgI CCRYGG 2 cut(s) 44, 105
BtsCI GGATG 1 cut(s) 514
Cac8I GCNNGC 1 cut(s) 140
Cfr13I GGNCC 2 cut(s) 409, 994
Cfr42I CCGCGG 1 cut(s) 47
Csp6I GTAC 1 cut(s) 393
CviAII CATG 1 cut(s) 982
CviQI GTAC 1 cut(s) 393
DdeI CTNAG 3 cut(s) 84, 941, 990
DpnI GATC 4 cut(s) 681, 765, 857, 906
DpnII GATC 4 cut(s) 679, 763, 855, 904
DraI TTTAAA 1 cut(s) 879
DraIII CACNNNGTG 1 cut(s) 364
EaeI YGGCCR 1 cut(s) 955
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
EciI GGCGGA 1 cut(s) 521
Eco130I CCWWGG 3 cut(s) 378, 758, 958
Eco24I GRGCYC 1 cut(s) 193
Eco31I GGTCTC 1 cut(s) 514
Eco47I GGWCC 2 cut(s) 409, 994
Eco88I CYCGRG 1 cut(s) 386
EcoO109I RGGNCCY 1 cut(s) 994
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 717
EcoT14I CCWWGG 3 cut(s) 378, 758, 958
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 3 cut(s) 378, 758, 958
FaeI CATG 1 cut(s) 985
FaiI YATR 7 cut(s) 176, 186, 494, 618, 632, 978, 983
FatI CATG 1 cut(s) 981
Fnu4HI GCNGC 3 cut(s) 44, 47, 101
FokI GGATG 1 cut(s) 521
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 3 cut(s) 44, 47, 101
FspBI CTAG 3 cut(s) 773, 933, 1082
GluI GCNGC 3 cut(s) 44, 47, 101
HaeIII GGCC 2 cut(s) 449, 957
Hin1II CATG 1 cut(s) 985
HincII GTYRAC 2 cut(s) 604, 886
HindII GTYRAC 2 cut(s) 604, 886
HinfI GANTC 4 cut(s) 6, 123, 653, 844
HpaI GTTAAC 1 cut(s) 604
HphI GGTGA 4 cut(s) 130, 446, 583, 691
Hpy166II GTNNAC 2 cut(s) 604, 886
Hpy188I TCNGA 2 cut(s) 87, 1018
Hpy188III TCNNGA 5 cut(s) 407, 551, 677, 1027, 1060
Hpy8I GTNNAC 2 cut(s) 604, 886
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 4 cut(s) 206, 790, 895, 1066
HpyCH4IV ACGT 1 cut(s) 1012
HpyCH4V TGCA 2 cut(s) 138, 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 373, 468
HpyF3I CTNAG 3 cut(s) 84, 941, 990
HpySE526I ACGT 1 cut(s) 1012
Hsp92II CATG 1 cut(s) 985
KpnI GGTACC 1 cut(s) 396
KspAI GTTAAC 1 cut(s) 604
KspI CCGCGG 1 cut(s) 47
Kzo9I GATC 4 cut(s) 679, 763, 855, 904
LmnI GCTCC 2 cut(s) 534, 797
Lsp1109I GCAGC 1 cut(s) 87
MaeI CTAG 3 cut(s) 773, 933, 1082
MaeII ACGT 1 cut(s) 1012
MaeIII GTNAC 1 cut(s) 1060
MalI GATC 4 cut(s) 681, 765, 857, 906
MboI GATC 4 cut(s) 679, 763, 855, 904
MboII GAAGA 7 cut(s) 41, 44, 47, 325, 605, 751, 833
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 2 cut(s) 193, 369
MlsI TGGCCA 1 cut(s) 957
MluNI TGGCCA 1 cut(s) 957
MlyI GAGTC 2 cut(s) 15, 853
Mox20I TGGCCA 1 cut(s) 957
MscI TGGCCA 1 cut(s) 957
MseI TTAA 4 cut(s) 155, 420, 603, 878
MslI CAYNNNNRTG 2 cut(s) 648, 812
Msp20I TGGCCA 1 cut(s) 957
MspA1I CMGCKG 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 719
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 719
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 2 cut(s) 373, 468
NdeII GATC 4 cut(s) 679, 763, 855, 904
NlaIII CATG 1 cut(s) 985
NlaIV GGNNCC 3 cut(s) 384, 394, 799
NmeAIII GCCGAG 1 cut(s) 488
NmuCI GTSAC 1 cut(s) 1060
NspV TTCGAA 1 cut(s) 949
PasI CCCWGGG 1 cut(s) 718
PfeI GAWTC 2 cut(s) 123, 653
PkrI GCNGC 3 cut(s) 45, 48, 102
PleI GAGTC 2 cut(s) 14, 852
PpsI GAGTC 2 cut(s) 14, 852
PpuMI RGGWCCY 1 cut(s) 994
PsiI TTATAA 2 cut(s) 494, 632
Psp1406I AACGTT 1 cut(s) 1012
Psp5II RGGWCCY 1 cut(s) 994
Psp6I CCWGG 1 cut(s) 717
PspGI CCWGG 1 cut(s) 717
PspN4I GGNNCC 3 cut(s) 384, 394, 799
PspPI GGNCC 2 cut(s) 409, 994
PspPPI RGGWCCY 1 cut(s) 994
PsuI RGATCY 1 cut(s) 763
RsaI GTAC 1 cut(s) 394
RsaNI GTAC 1 cut(s) 393
RseI CAYNNNNRTG 2 cut(s) 648, 812
SacII CCGCGG 1 cut(s) 47
SaqAI TTAA 4 cut(s) 155, 420, 603, 878
SatI GCNGC 3 cut(s) 44, 47, 101
Sau3AI GATC 4 cut(s) 679, 763, 855, 904
Sau96I GGNCC 2 cut(s) 409, 994
SchI GAGTC 2 cut(s) 15, 853
ScrFI CCNGG 1 cut(s) 719
SduI GDGCHC 2 cut(s) 193, 369
Sfr303I CCGCGG 1 cut(s) 47
SfuI TTCGAA 1 cut(s) 949
SgrBI CCGCGG 1 cut(s) 47
SinI GGWCC 2 cut(s) 409, 994
SmiMI CAYNNNNRTG 2 cut(s) 648, 812
SmlI CTYRAG 3 cut(s) 327, 998, 1025
SmoI CTYRAG 3 cut(s) 327, 998, 1025
SsiI CCGC 3 cut(s) 44, 46, 532
SspMI CTAG 3 cut(s) 773, 933, 1082
StyD4I CCNGG 1 cut(s) 717
StyI CCWWGG 3 cut(s) 378, 758, 958
TaaI ACNGT 4 cut(s) 206, 790, 895, 1066
TaiI ACGT 1 cut(s) 1015
TaqI TCGA 4 cut(s) 18, 276, 847, 949
TauI GCSGC 2 cut(s) 46, 49
TfiI GAWTC 2 cut(s) 123, 653
Tru1I TTAA 4 cut(s) 155, 420, 603, 878
Tru9I TTAA 4 cut(s) 155, 420, 603, 878
TseFI GTSAC 1 cut(s) 1060
TseI GCWGC 1 cut(s) 100
Tsp45I GTSAC 1 cut(s) 1060
TspDTI ATGAA 4 cut(s) 163, 263, 525, 654
TspGWI ACGGA 1 cut(s) 917
VpaK11BI GGWCC 2 cut(s) 409, 994
XapI RAATTY 5 cut(s) 544, 666, 733, 874, 1019
XcmI CCANNNNNNNNNTGG 2 cut(s) 403, 808
XspI CTAG 3 cut(s) 773, 933, 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.