Rorug03G0170100

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003503
Physical Location & Seq
Forward (+)
14371555 .. 14373245
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0170100.1

Sequence Viewer

Length: 528 bp
ATGTGGGAGTTCCGTAGCCCAAAGGGAGCATCCCATTCCCACTGCTGGTTGTGCTTTCGCAGAAACAAGAAGAAGAAGAAGAAAACGAAGGAATCATCATCAGATATGGCTCGGAGCATCTCCTACATAACCGGGTCTCAGCTCCTCTCTCTCAGACGCCGCCCCAACAATATCGCCATCATCGATGTCAGGGATGATGAAAGGAGCTACGATGGGCATATAGCAGGGTCTTTGCACTACCCCAATGACAGTTTCTATGACAAGATCTCCAATCTCGCTCAAGAAGTCAAAGGCAAAGACACCCTCGTTTTCCACTGCGCTCTCAGCCAGGTCCGAGGCCCGTCCTGTGCGCGAAAGTTTGCCAATTATCTTGAGGAGATGAAGGAAGATACAGGAATCAAAGACATTTTGGTTTTGGAGCGTGGCTTCAATGGCTGGGAATCTTCTGGTAGACCTGTTTGTCGCTGCAACAGCATTCCCTGTAAGGGTGAGATTCAGACTGAGACTGAGACTGAGAAGAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

175

Amino Acids

19.99

Weight (kDa)

9.17

Isoelectric Point (pI)

63.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 50 - 148 8.5e-12 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 459
AccI GTMKAC 1 cut(s) 451
AccII CGCG 1 cut(s) 352
AciI CCGC 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 157
AfiI CCNNNNNNNGG 2 cut(s) 45, 485
AgsI TTSAA 1 cut(s) 430
AjnI CCWGG 1 cut(s) 327
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 2 cut(s) 142, 207
AluI AGCT 2 cut(s) 142, 207
Alw26I GTCTC 3 cut(s) 141, 497, 503
AoxI GGCC 1 cut(s) 337
ApeKI GCWGC 1 cut(s) 465
AspLEI GCGC 2 cut(s) 320, 352
AspS9I GGNCC 2 cut(s) 331, 338
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 500
AvaII GGWCC 1 cut(s) 331
BbvI GCAGC 1 cut(s) 452
BccI CCATC 2 cut(s) 185, 206
BciT130I CCWGG 1 cut(s) 329
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 3 cut(s) 141, 497, 503
BglII AGATCT 1 cut(s) 264
BisI GCNGC 2 cut(s) 160, 466
BlsI GCNGC 2 cut(s) 161, 467
Bme1390I CCNGG 2 cut(s) 133, 329
Bme18I GGWCC 1 cut(s) 331
BmgT120I GGNCC 2 cut(s) 331, 338
BmrFI CCNGG 2 cut(s) 133, 329
BmsI GCATC 2 cut(s) 38, 126
BpuEI CTTGAG 2 cut(s) 264, 392
BpuMI CCSGG 1 cut(s) 133
Bsa29I ATCGAT 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 157
BsaI GGTCTC 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 334
BsaXI ACNNNNNCTCC 2 cut(s) 251, 281
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 485
BseBI CCWGG 1 cut(s) 329
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 2 cut(s) 29, 199
BseLI CCNNNNNNNGG 2 cut(s) 45, 485
BseMII CTCAG 6 cut(s) 152, 166, 337, 492, 498, 504
BseRI GAGGAG 2 cut(s) 134, 389
BseXI GCAGC 1 cut(s) 452
BseYI CCCAGC 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 352
BshFI GGCC 1 cut(s) 339
BshVI ATCGAT 1 cut(s) 183
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 2 cut(s) 45, 485
BsmAI GTCTC 3 cut(s) 141, 497, 503
BsmI GAATGC 1 cut(s) 474
BsnI GGCC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 141
Bsp143I GATC 1 cut(s) 264
BspACI CCGC 1 cut(s) 160
BspANI GGCC 1 cut(s) 339
BspCNI CTCAG 6 cut(s) 151, 165, 336, 493, 499, 505
BspDI ATCGAT 1 cut(s) 183
BspFNI CGCG 1 cut(s) 352
BspTNI GGTCTC 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 1 cut(s) 264
BssNI GRCGYC 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 251
BstACI GRCGYC 1 cut(s) 157
BstDEI CTNAG 6 cut(s) 138, 152, 323, 501, 507, 513
BstF5I GGATG 2 cut(s) 29, 199
BstFNI CGCG 1 cut(s) 352
BstHHI GCGC 2 cut(s) 320, 352
BstKTI GATC 1 cut(s) 267
BstMAI GTCTC 3 cut(s) 141, 497, 503
BstMBI GATC 1 cut(s) 264
BstMWI GCNNNNNNNGC 4 cut(s) 51, 324, 432, 471
BstNI CCWGG 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 131, 327
BstUI CGCG 1 cut(s) 352
BstV1I GCAGC 1 cut(s) 452
BstX2I RGATCY 1 cut(s) 264
BstYI RGATCY 1 cut(s) 264
Bsu15I ATCGAT 1 cut(s) 183
BsuRI GGCC 1 cut(s) 339
BsuTUI ATCGAT 1 cut(s) 183
BtsCI GGATG 2 cut(s) 29, 199
BtsI GCAGTG 2 cut(s) 40, 313
BtsIMutI CAGTG 2 cut(s) 40, 313
CfoI GCGC 2 cut(s) 320, 352
Cfr13I GGNCC 2 cut(s) 331, 338
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 1 cut(s) 165
CviJI RGCY 8 cut(s) 18, 110, 142, 207, 327, 339, 426, 435
CviKI_1 RGCY 8 cut(s) 18, 110, 142, 207, 327, 339, 426, 435
DdeI CTNAG 6 cut(s) 138, 152, 323, 501, 507, 513
DpnI GATC 1 cut(s) 266
DpnII GATC 1 cut(s) 264
DrdI GACNNNNNNGTC 1 cut(s) 459
DseDI GACNNNNNNGTC 1 cut(s) 459
Eco31I GGTCTC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 327
FaiI YATR 6 cut(s) 107, 128, 219, 221, 258, 526
FblI GTMKAC 1 cut(s) 451
Fnu4HI GCNGC 2 cut(s) 160, 466
FokI GGATG 2 cut(s) 16, 206
Fsp4HI GCNGC 2 cut(s) 160, 466
GlaI GCGC 2 cut(s) 319, 351
GluI GCNGC 2 cut(s) 160, 466
GsaI CCCAGC 1 cut(s) 439
HaeIII GGCC 1 cut(s) 339
HapII CCGG 1 cut(s) 132
HgaI GACGC 1 cut(s) 165
HhaI GCGC 2 cut(s) 320, 352
Hin1I GRCGYC 1 cut(s) 157
Hin6I GCGC 2 cut(s) 318, 350
HinP1I GCGC 2 cut(s) 318, 350
HinfI GANTC 4 cut(s) 92, 396, 440, 493
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 5 cut(s) 103, 114, 155, 335, 498
Hpy188III TCNNGA 2 cut(s) 281, 371
Hpy8I GTNNAC 1 cut(s) 452
HpyAV CCTTC 2 cut(s) 82, 376
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4V TGCA 2 cut(s) 235, 468
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 324, 432, 471
HpyF3I CTNAG 6 cut(s) 138, 152, 323, 501, 507, 513
Hsp92I GRCGYC 1 cut(s) 157
HspAI GCGC 2 cut(s) 318, 350
Kzo9I GATC 1 cut(s) 264
LmnI GCTCC 5 cut(s) 26, 114, 147, 204, 418
Lsp1109I GCAGC 1 cut(s) 452
LweI GCATC 2 cut(s) 38, 126
MalI GATC 1 cut(s) 266
MboI GATC 1 cut(s) 264
MboII GAAGA 6 cut(s) 82, 85, 88, 91, 398, 435
MflI RGATCY 1 cut(s) 264
MluCI AATT 1 cut(s) 364
MnlI CCTC 4 cut(s) 155, 314, 329, 367
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 133, 329
Mva1269I GAATGC 1 cut(s) 474
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 1 cut(s) 352
MwoI GCNNNNNNNGC 4 cut(s) 51, 324, 432, 471
NciI CCSGG 1 cut(s) 133
NdeII GATC 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 474
PfeI GAWTC 4 cut(s) 92, 396, 440, 493
PkrI GCNGC 2 cut(s) 161, 467
Psp6I CCWGG 1 cut(s) 327
PspFI CCCAGC 1 cut(s) 435
PspGI CCWGG 1 cut(s) 327
PspPI GGNCC 2 cut(s) 331, 338
PsuI RGATCY 1 cut(s) 264
SatI GCNGC 2 cut(s) 160, 466
Sau3AI GATC 1 cut(s) 264
Sau96I GGNCC 2 cut(s) 331, 338
ScrFI CCNGG 2 cut(s) 133, 329
SetI ASST 4 cut(s) 144, 209, 333, 457
SfaNI GCATC 2 cut(s) 38, 126
SinI GGWCC 1 cut(s) 331
SmlI CTYRAG 2 cut(s) 279, 371
SmoI CTYRAG 2 cut(s) 279, 371
Sse9I AATT 1 cut(s) 364
SsiI CCGC 1 cut(s) 160
StyD4I CCNGG 2 cut(s) 131, 327
TaaI ACNGT 1 cut(s) 251
TaqI TCGA 1 cut(s) 183
TasI AATT 1 cut(s) 364
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 4 cut(s) 92, 396, 440, 493
TscAI CASTG 2 cut(s) 47, 320
TseI GCWGC 1 cut(s) 465
TspDTI ATGAA 2 cut(s) 213, 395
TspRI CASTG 2 cut(s) 47, 320
VpaK11BI GGWCC 1 cut(s) 331
XmiI GTMKAC 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.