MD14G1052400.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003503
Physical Location & Seq
Forward (+)
5060912 .. 5061502
591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1052400.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGCCAAGACCCTCCAAAACCTCAGTTCTACCTACACCATTCTCATAACCACTTCCTTCTTCTTCCTCTCAACCCTTGTCACATCAGAAACTCACCGTTTTATAAGATCGTTATCTTCATCAAAACATGGTTTAAAGAGAGAGAAACTAAGCCATGTTCACTTCTACTTCCATGACATAGTGAGTGGCCGAACCCCCACGGCAGTTAAAGTGGCTGACGCACCCACGACAAACACTTCCATGACTGGCTTTGGCACTGTGTTCATGATGGACGATCCATTGACTATCAGCCCTGAATCGAGCTCTAAGCTAGTCGGAAAAGCCCAAGGTATTTATGCTTCAGCTTCACAAAGTGAGTTGGGGCTTTTGATGGCCTTGAATTTTGTTTTTGTTGAACGGAAGTACAATGGTAGCACTCTTAGTGTATTGGGGCGCAACACCGTGTTCTCAGCCATGAGGGAGATGCCGATAGTAGGCGGAAGTGGGCTTTTCCGATTTGCTCGGGGCTATGCTCATGCTAGCACTCACCAGTTTGACATCAAAACTGGAGATGCTGTTGTGGAGTATAATGTCTATGTCTTCCATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

197

Amino Acids

21.63

Weight (kDa)

9.49

Isoelectric Point (pI)

28.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 51 - 194 3.3e-59 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 104
AciI CCGC 1 cut(s) 477
AclWI GGATC 1 cut(s) 269
AcoI YGGCCR 2 cut(s) 3, 187
AcsI RAATTY 1 cut(s) 379
AcuI CTGAAG 1 cut(s) 324
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 2 cut(s) 379, 395
AluBI AGCT 3 cut(s) 303, 310, 344
AluI AGCT 3 cut(s) 303, 310, 344
Alw21I GWGCWC 1 cut(s) 305
AlwI GGATC 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 501
AoxI GGCC 3 cut(s) 3, 187, 372
ApoI RAATTY 1 cut(s) 379
AspLEI GCGC 1 cut(s) 435
AsuHPI GGTGA 2 cut(s) 86, 518
AsuNHI GCTAGC 1 cut(s) 518
AvaI CYCGRG 1 cut(s) 501
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 305
BarI GAAGNNNNNNTAC 2 cut(s) 322, 354
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 1 cut(s) 305
BccI CCATC 2 cut(s) 262, 364
BceAI ACGGC 1 cut(s) 216
BfaI CTAG 2 cut(s) 311, 519
BmeT110I CYCGRG 1 cut(s) 501
BmsI GCATC 2 cut(s) 453, 541
BmtI GCTAGC 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 567
BsaBI GATNNNNATC 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 198, 325
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse1I ACTGG 3 cut(s) 250, 529, 550
Bse8I GATNNNNATC 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 198, 325
BseJI GATNNNNATC 1 cut(s) 112
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMII CTCAG 2 cut(s) 37, 462
BseNI ACTGG 3 cut(s) 250, 529, 550
BshFI GGCC 3 cut(s) 5, 189, 374
BsiHKAI GWGCWC 1 cut(s) 305
BsiHKCI CYCGRG 1 cut(s) 501
BslI CCNNNNNNNGG 1 cut(s) 473
BsnI GGCC 3 cut(s) 5, 189, 374
BsoBI CYCGRG 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 305
Bsp143I GATC 2 cut(s) 107, 274
BspACI CCGC 1 cut(s) 477
BspANI GGCC 3 cut(s) 5, 189, 374
BspCNI CTCAG 2 cut(s) 36, 461
BspHI TCATGA 1 cut(s) 264
BspOI GCTAGC 1 cut(s) 522
BspPI GGATC 1 cut(s) 269
BsrI ACTGG 3 cut(s) 250, 529, 550
BssECI CCNNGG 2 cut(s) 198, 325
BssMI GATC 2 cut(s) 107, 274
BssT1I CCWWGG 1 cut(s) 325
Bst4CI ACNGT 3 cut(s) 98, 259, 442
BstC8I GCNNGC 1 cut(s) 520
BstDEI CTNAG 5 cut(s) 23, 149, 306, 419, 448
BstDSI CCRYGG 1 cut(s) 198
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 2 cut(s) 110, 277
BstMBI GATC 2 cut(s) 107, 274
BstV2I GAAGAC 1 cut(s) 571
BsuRI GGCC 3 cut(s) 5, 189, 374
BtgI CCRYGG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 520
CciI TCATGA 1 cut(s) 264
CfoI GCGC 1 cut(s) 435
CseI GACGC 1 cut(s) 227
Csp6I GTAC 1 cut(s) 403
CviAII CATG 7 cut(s) 128, 155, 173, 241, 265, 454, 515
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 5 cut(s) 23, 149, 306, 419, 448
DpnI GATC 2 cut(s) 109, 276
DpnII GATC 2 cut(s) 107, 274
DraI TTTAAA 1 cut(s) 135
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 2 cut(s) 3, 187
EciI GGCGGA 1 cut(s) 492
Ecl136II GAGCTC 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 303
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 7 cut(s) 131, 158, 176, 244, 268, 457, 518
FatI CATG 7 cut(s) 127, 154, 172, 240, 264, 453, 514
FriOI GRGCYC 1 cut(s) 305
FspBI CTAG 2 cut(s) 311, 519
GlaI GCGC 1 cut(s) 434
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 3 cut(s) 5, 189, 374
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 435
Hin1II CATG 7 cut(s) 131, 158, 176, 244, 268, 457, 518
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HinfI GANTC 1 cut(s) 296
HphI GGTGA 2 cut(s) 86, 518
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 3 cut(s) 88, 317, 494
Hpy188III TCNNGA 1 cut(s) 265
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 3 cut(s) 98, 259, 442
HpyF3I CTNAG 5 cut(s) 23, 149, 306, 419, 448
Hsp92II CATG 7 cut(s) 131, 158, 176, 244, 268, 457, 518
HspAI GCGC 1 cut(s) 433
Kzo9I GATC 2 cut(s) 107, 274
LpnPI CCDG 4 cut(s) 231, 306, 531, 542
LweI GCATC 2 cut(s) 453, 541
MaeI CTAG 2 cut(s) 311, 519
MaeIII GTNAC 1 cut(s) 79
MalI GATC 2 cut(s) 109, 276
MboI GATC 2 cut(s) 107, 274
MboII GAAGA 4 cut(s) 52, 55, 108, 571
MhlI GDGCHC 1 cut(s) 305
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 379
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 4 cut(s) 23, 32, 77, 450
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 134, 207, 589
MslI CAYNNNNRTG 1 cut(s) 239
Msp20I TGGCCA 1 cut(s) 5
NdeII GATC 2 cut(s) 107, 274
NheI GCTAGC 1 cut(s) 518
NlaIII CATG 7 cut(s) 131, 158, 176, 244, 268, 457, 518
NmuCI GTSAC 1 cut(s) 79
PagI TCATGA 1 cut(s) 264
PfeI GAWTC 1 cut(s) 296
PsiI TTATAA 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 305
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 305
SaqAI TTAA 3 cut(s) 134, 207, 589
Sau3AI GATC 2 cut(s) 107, 274
SduI GDGCHC 1 cut(s) 305
SetI ASST 6 cut(s) 24, 35, 305, 312, 331, 346
SfaNI GCATC 2 cut(s) 453, 541
SmiMI CAYNNNNRTG 1 cut(s) 239
Sse9I AATT 1 cut(s) 379
SsiI CCGC 1 cut(s) 477
SspMI CTAG 2 cut(s) 311, 519
SstI GAGCTC 1 cut(s) 305
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 3 cut(s) 98, 259, 442
TaqI TCGA 1 cut(s) 299
TasI AATT 1 cut(s) 379
TatI WGTACW 1 cut(s) 402
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 134, 207, 589
Tru9I TTAA 3 cut(s) 134, 207, 589
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 79
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 2 cut(s) 108, 253
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 1 cut(s) 262
XapI RAATTY 1 cut(s) 379
XspI CTAG 2 cut(s) 311, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.