Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005731

Physical Location & Seq

Genomic Coordinates Forward (+)

25493193 .. 25496053

Gene Structure 2861 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G68010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGGCGAAACCGGTGTCCATTGAAGTGTATAATCCTAATGGGAAATACAGAGTTGTTAGCACAAAACCGATGCCTGGAACTCGCTGGATCAATCTCTTGGTAGACCAAGGTTGTCGCGTTGAGATATGTCATTTGAAGAAGACAATCTTGTCTGTAGAAGATATCATTGATCTGATCGGAGACAAGTGTGATGGAGTCATCGGTCAGTTGACGGAAGATTGGGGAGAGACTCTGTTCTCAGCTTTGAGCAAAGCTGGAGGGAAAGCTTTCAGTAACATGGCCGTTGGTTATAACAACGTTGATGTTGAAGCTGCCAATAAGTATGGAATTGCTGTCGGTAACACTCCGGGAGTGTTGACTGAGACGACGGCTGAACTAGCTGCTTCTCTTTCCTTGGCTGCTGCAAGAAGAATTGTTGAAGCCGACGAATTCATGAGAGGTGGCTTGTACGAGGGATGGCTTCCTCATCTGTTTGTGGGGAACTTACTTAAAGGACAGACTGTTGGAGTTATTGGAGCTGGACGTATTGGATCTGCTTATGCTAGAATGATGGTGGAAGGGTTCAAGATGAATTTGATCTACTTTGATCTTTACCAATCCACTCGTCTTGAGAAATTTGTGACAGCTTATGGACAGTTCTTGAAAGCAAATGGAGAACAACCTGTGACATGGAAACGAGCTTCGTCCATGGAGGAGGTGCTGCGTGAGGCTGATCTGATAAGTCTTCACCCGGTGCTGGACAAAACCACTTACCATCTTGTCAACAAGGAGAGGCTTGCCATGATGAAAAAGGAAGCAATCCTTGTGAACTGCAGCAGAGGTCCTGTGATCGATGAGGCAGCTTTGGTCGAACATCTCAAAGAGAACCCGATGTTCCGAGTTGGTCTCGATGTGTTCGAGGAAGAGCCATTCATGAAACCAGGGCTTGCTGATACGAAAAACGCTATTGTTGTTCCTCACATTGCTTCTGCTTCCAAGTGGACTCGTGAAGGAATGGCTACGCTTGCAGCTCTCAACGTCCTCGGAAGAGTCAAAGGGTACCCGATTTGGCATGACCCGAACCGAGTCGATCCATTCTTGAACGAAAACGCTTCACCGCCCAATGCCAGTCCAAGCATCGTCAACTCAAAGGCCTTAGGATTGCCTGTTTCGAAGCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0008465 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009536 GO:0009605 GO:0009628 GO:0009853 GO:0009854 GO:0009987 GO:0009991 GO:0010035 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0030267 GO:0031668 GO:0033554 GO:0042221 GO:0042579 GO:0042631 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050896 GO:0051716 GO:0055114 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071478 GO:0071482 GO:0071496 GO:0097159 GO:0104004 GO:1901363 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

386

Amino Acids

42.25

Weight (kDa)

6.68

Isoelectric Point (pI)

28.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	30 - 346	6.9e-18	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	130 - 322	1.3e-44	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68010 AT1G68010 AT1G68010
fragaria_vesca	FvH4_5g30340 FvH4_5g30340
malus_domestica	MD05G1122100.v1.1
prunus_persica	Prupe.7G264000_v2.0.a1 Prupe.8G166000_v2.0.a1 Prupe.8G166000_v2.0.a1
pyrus_communis	pycom05g11630 pycom10g10740
rosa_chinensis	RchiOBHm_Chr7g0224721 RchiOBHm_Chr7g0224761
rosa_laevigata	RLG00000001858 RLG00000001862
rosa_multiflora	Rmu_sc0000144.1_g000017 Rmu_sc0000144.1_g000025
rosa_roxburghii	Rroxscaffold_3G00234460
rosa_rugosa	Rorug07G0225200 Rorug07G0225400
rosa_samantha	Rh7AG377600 Rh7AG377700 Rh7BG361200 Rh7BG361500 Rh7CG396400 Rh7CG396600 Rh7DG371300
rosa_wichuraiana	Rw7G031340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	291
AasI	GACNNNNNNGTC	1 cut(s)	148
Acc65I	GGTACC	1 cut(s)	1038
AccB1I	GGYRCC	1 cut(s)	1038
AccI	GTMKAC	1 cut(s)	102
AccII	CGCG	1 cut(s)	117
AciI	CCGC	1 cut(s)	1097
AclI	AACGTT	1 cut(s)	297
AclWI	GGATC	3 cut(s)	95, 538, 1064
AcoI	YGGCCR	1 cut(s)	279
AcsI	RAATTY	3 cut(s)	428, 571, 614
AdeI	CACNNNGTG	1 cut(s)	733
AfaI	GTAC	2 cut(s)	449, 1040
AfiI	CCNNNNNNNGG	2 cut(s)	74, 736
AgeI	ACCGGT	1 cut(s)	10
AgsI	TTSAA	7 cut(s)	23, 136, 308, 419, 565, 643, 1081
AjnI	CCWGG	2 cut(s)	73, 919
Alw26I	GTCTC	4 cut(s)	174, 221, 356, 890
AlwI	GGATC	3 cut(s)	95, 538, 1064
AoxI	GGCC	2 cut(s)	279, 1131
ApeKI	GCWGC	8 cut(s)	311, 380, 398, 401, 700, 813, 839, 1007
ApoI	RAATTY	3 cut(s)	428, 571, 614
ArsI	GACNNNNNNTTYG	1 cut(s)	31
AsiGI	ACCGGT	1 cut(s)	10
Asp700I	GAANNNNTTC	1 cut(s)	266
Asp718I	GGTACC	1 cut(s)	1038
AspS9I	GGNCC	1 cut(s)	821
AsuC2I	CCSGG	2 cut(s)	348, 731
AsuHPI	GGTGA	2 cut(s)	719, 1086
AsuII	TTCGAA	1 cut(s)	1151
AvaII	GGWCC	1 cut(s)	821
AxyI	CCTNAGG	1 cut(s)	1135
BanI	GGYRCC	1 cut(s)	1038
BauI	CACGAG	1 cut(s)	984
BbsI	GAAGAC	2 cut(s)	146, 716
BbvI	GCAGC	8 cut(s)	298, 367, 385, 388, 687, 825, 851, 1019
BccI	CCATC	4 cut(s)	185, 450, 544, 762
BceAI	ACGGC	2 cut(s)	266, 384
BciT130I	CCWGG	2 cut(s)	75, 921
BcnI	CCSGG	2 cut(s)	348, 731
BcoDI	GTCTC	4 cut(s)	174, 221, 356, 890
BfaI	CTAG	2 cut(s)	377, 543
BfmI	CTRYAG	2 cut(s)	153, 811
BisI	GCNGC	8 cut(s)	312, 381, 399, 402, 701, 814, 840, 1008
BlsI	GCNGC	8 cut(s)	313, 382, 400, 403, 702, 815, 841, 1009
Bme1390I	CCNGG	4 cut(s)	75, 348, 731, 921
Bme18I	GGWCC	1 cut(s)	821
BmgT120I	GGNCC	1 cut(s)	821
BmiI	GGNNCC	1 cut(s)	1040
BmrFI	CCNGG	4 cut(s)	75, 348, 731, 921
BmsI	GCATC	2 cut(s)	60, 1125
BpiI	GAAGAC	2 cut(s)	146, 716
BplI	GAGNNNNNCTC	2 cut(s)	870, 902
BpmI	CTGGAG	1 cut(s)	276
Bpu14I	TTCGAA	1 cut(s)	1151
BpuEI	CTTGAG	1 cut(s)	629
BpuMI	CCSGG	2 cut(s)	348, 731
Bsa29I	ATCGAT	1 cut(s)	831
BsaI	GGTCTC	1 cut(s)	890
BsaJI	CCNNGG	5 cut(s)	106, 393, 687, 920, 1021
BsaWI	WCCGGW	1 cut(s)	10
Bsc4I	CCNNNNNNNGG	2 cut(s)	74, 736
Bse118I	RCCGGY	1 cut(s)	10
Bse1I	ACTGG	1 cut(s)	1107
Bse21I	CCTNAGG	1 cut(s)	1135
Bse3DI	GCAATG	1 cut(s)	960
BseBI	CCWGG	2 cut(s)	75, 921
BseCI	ATCGAT	1 cut(s)	831
BseDI	CCNNGG	5 cut(s)	106, 393, 687, 920, 1021
BseGI	GGATG	1 cut(s)	461
BseLI	CCNNNNNNNGG	2 cut(s)	74, 736
BseMI	GCAATG	1 cut(s)	960
BseMII	CTCAG	2 cut(s)	252, 351
BseNI	ACTGG	1 cut(s)	1107
BseRI	GAGGAG	1 cut(s)	707
BseXI	GCAGC	8 cut(s)	298, 367, 385, 388, 687, 825, 851, 1019
Bsh1236I	CGCG	1 cut(s)	117
BshFI	GGCC	2 cut(s)	281, 1133
BshNI	GGYRCC	1 cut(s)	1038
BshTI	ACCGGT	1 cut(s)	10
BshVI	ATCGAT	1 cut(s)	831
BsiSI	CCGG	3 cut(s)	11, 347, 731
BslI	CCNNNNNNNGG	2 cut(s)	74, 736
BsmAI	GTCTC	4 cut(s)	174, 221, 356, 890
BsmBI	CGTCTC	1 cut(s)	356
BsnI	GGCC	2 cut(s)	281, 1133
Bso31I	GGTCTC	1 cut(s)	890
Bsp119I	TTCGAA	1 cut(s)	1151
Bsp143I	GATC	9 cut(s)	87, 169, 174, 530, 576, 586, 712, 828, 1069
Bsp19I	CCATGG	1 cut(s)	687
BspACI	CCGC	1 cut(s)	1097
BspANI	GGCC	2 cut(s)	281, 1133
BspCNI	CTCAG	2 cut(s)	251, 352
BspDI	ATCGAT	1 cut(s)	831
BspFNI	CGCG	1 cut(s)	117
BspHI	TCATGA	2 cut(s)	432, 912
BspLI	GGNNCC	1 cut(s)	1040
BspMAI	CTGCAG	1 cut(s)	815
BspPI	GGATC	3 cut(s)	95, 538, 1064
BspQI	GCTCTTC	1 cut(s)	897
BspT104I	TTCGAA	1 cut(s)	1151
BspT107I	GGYRCC	1 cut(s)	1038
BspTNI	GGTCTC	1 cut(s)	890
BsrDI	GCAATG	1 cut(s)	960
BsrFI	RCCGGY	1 cut(s)	10
BsrI	ACTGG	1 cut(s)	1107
BssAI	RCCGGY	1 cut(s)	10
BssECI	CCNNGG	5 cut(s)	106, 393, 687, 920, 1021
BssMI	GATC	9 cut(s)	87, 169, 174, 530, 576, 586, 712, 828, 1069
BssSI	CACGAG	1 cut(s)	984
BssT1I	CCWWGG	3 cut(s)	106, 393, 687
Bst2BI	CACGAG	1 cut(s)	984
Bst2UI	CCWGG	2 cut(s)	75, 921
Bst4CI	ACNGT	2 cut(s)	502, 636
Bst6I	CTCTTC	2 cut(s)	897, 1021
BstBI	TTCGAA	1 cut(s)	1151
BstC8I	GCNNGC	3 cut(s)	777, 927, 1005
BstDEI	CTNAG	3 cut(s)	238, 360, 1135
BstDSI	CCRYGG	1 cut(s)	687
BstF5I	GGATG	1 cut(s)	461
BstFNI	CGCG	1 cut(s)	117
BstKTI	GATC	9 cut(s)	90, 172, 177, 533, 579, 589, 715, 831, 1072
BstMAI	GTCTC	4 cut(s)	174, 221, 356, 890
BstMBI	GATC	9 cut(s)	87, 169, 174, 530, 576, 586, 712, 828, 1069
BstMWI	GCNNNNNNNGC	2 cut(s)	377, 1004
BstNI	CCWGG	2 cut(s)	75, 921
BstSCI	CCNGG	4 cut(s)	73, 346, 729, 919
BstSFI	CTRYAG	2 cut(s)	153, 811
BstUI	CGCG	1 cut(s)	117
BstV1I	GCAGC	8 cut(s)	298, 367, 385, 388, 687, 825, 851, 1019
BstV2I	GAAGAC	2 cut(s)	146, 716
BstX2I	RGATCY	1 cut(s)	530
BstYI	RGATCY	1 cut(s)	530
Bsu15I	ATCGAT	1 cut(s)	831
Bsu36I	CCTNAGG	1 cut(s)	1135
BsuRI	GGCC	2 cut(s)	281, 1133
BsuTUI	ATCGAT	1 cut(s)	831
BtgI	CCRYGG	1 cut(s)	687
BtsCI	GGATG	1 cut(s)	461
Cac8I	GCNNGC	3 cut(s)	777, 927, 1005
CciI	TCATGA	2 cut(s)	432, 912
Cfr10I	RCCGGY	1 cut(s)	10
Cfr13I	GGNCC	1 cut(s)	821
ClaI	ATCGAT	1 cut(s)	831
Csp6I	GTAC	2 cut(s)	448, 1039
CspAI	ACCGGT	1 cut(s)	10
CspCI	CAANNNNNGTGG	2 cut(s)	589, 624
CviAII	CATG	7 cut(s)	277, 433, 669, 688, 781, 913, 1052
CviQI	GTAC	2 cut(s)	448, 1039
DdeI	CTNAG	3 cut(s)	238, 360, 1135
DpnI	GATC	9 cut(s)	89, 171, 176, 532, 578, 588, 714, 830, 1071
DpnII	GATC	9 cut(s)	87, 169, 174, 530, 576, 586, 712, 828, 1069
DraIII	CACNNNGTG	1 cut(s)	733
DrdI	GACNNNNNNGTC	1 cut(s)	148
DseDI	GACNNNNNNGTC	1 cut(s)	148
EaeI	YGGCCR	1 cut(s)	279
Eam1104I	CTCTTC	2 cut(s)	897, 1021
EarI	CTCTTC	2 cut(s)	897, 1021
Eco130I	CCWWGG	3 cut(s)	106, 393, 687
Eco147I	AGGCCT	1 cut(s)	1133
Eco31I	GGTCTC	1 cut(s)	890
Eco32I	GATATC	1 cut(s)	163
Eco47I	GGWCC	1 cut(s)	821
Eco81I	CCTNAGG	1 cut(s)	1135
EcoO109I	RGGNCCY	1 cut(s)	821
EcoRI	GAATTC	1 cut(s)	428
EcoRII	CCWGG	2 cut(s)	73, 919
EcoRV	GATATC	1 cut(s)	163
EcoT14I	CCWWGG	3 cut(s)	106, 393, 687
ErhI	CCWWGG	3 cut(s)	106, 393, 687
Esp3I	CGTCTC	1 cut(s)	356
FaeI	CATG	7 cut(s)	280, 436, 672, 691, 784, 916, 1055
FalI	AAGNNNNNCTT	4 cut(s)	131, 163, 786, 818
FatI	CATG	7 cut(s)	276, 432, 668, 687, 780, 912, 1051
FblI	GTMKAC	1 cut(s)	102
Fnu4HI	GCNGC	8 cut(s)	312, 381, 399, 402, 701, 814, 840, 1008
FokI	GGATG	1 cut(s)	468
Fsp4HI	GCNGC	8 cut(s)	312, 381, 399, 402, 701, 814, 840, 1008
FspBI	CTAG	2 cut(s)	377, 543
GluI	GCNGC	8 cut(s)	312, 381, 399, 402, 701, 814, 840, 1008
GsuI	CTGGAG	1 cut(s)	276
HaeIII	GGCC	2 cut(s)	281, 1133
HapII	CCGG	3 cut(s)	11, 347, 731
Hin1II	CATG	7 cut(s)	280, 436, 672, 691, 784, 916, 1055
HincII	GTYRAC	4 cut(s)	210, 357, 763, 1123
HindII	GTYRAC	4 cut(s)	210, 357, 763, 1123
HindIII	AAGCTT	1 cut(s)	264
HinfI	GANTC	5 cut(s)	195, 229, 982, 1029, 1065
HpaII	CCGG	3 cut(s)	11, 347, 731
HphI	GGTGA	2 cut(s)	719, 1086
Hpy166II	GTNNAC	7 cut(s)	103, 210, 357, 763, 808, 981, 1123
Hpy188I	TCNGA	5 cut(s)	174, 179, 717, 878, 1025
Hpy188III	TCNNGA	8 cut(s)	433, 565, 608, 640, 887, 913, 986, 1078
Hpy8I	GTNNAC	7 cut(s)	103, 210, 357, 763, 808, 981, 1123
Hpy99I	CGWCG	2 cut(s)	370, 428
HpyAV	CCTTC	2 cut(s)	551, 983
HpyCH4III	ACNGT	2 cut(s)	502, 636
HpyCH4IV	ACGT	3 cut(s)	297, 523, 1017
HpyCH4V	TGCA	3 cut(s)	404, 813, 1007
HpyF10VI	GCNNNNNNNGC	2 cut(s)	377, 1004
HpyF3I	CTNAG	3 cut(s)	238, 360, 1135
HpySE526I	ACGT	3 cut(s)	297, 523, 1017
Hsp92II	CATG	7 cut(s)	280, 436, 672, 691, 784, 916, 1055
KpnI	GGTACC	1 cut(s)	1042
Kzo9I	GATC	9 cut(s)	87, 169, 174, 530, 576, 586, 712, 828, 1069
LguI	GCTCTTC	1 cut(s)	897
LmnI	GCTCC	1 cut(s)	515
Lsp1109I	GCAGC	8 cut(s)	298, 367, 385, 388, 687, 825, 851, 1019
LweI	GCATC	2 cut(s)	60, 1125
MaeI	CTAG	2 cut(s)	377, 543
MaeII	ACGT	3 cut(s)	297, 523, 1017
MaeIII	GTNAC	4 cut(s)	272, 338, 619, 664
MalI	GATC	9 cut(s)	89, 171, 176, 532, 578, 588, 714, 830, 1071
MboI	GATC	9 cut(s)	87, 169, 174, 530, 576, 586, 712, 828, 1069
MboII	GAAGA	8 cut(s)	148, 151, 170, 227, 420, 716, 914, 1038
MflI	RGATCY	1 cut(s)	530
MluCI	AATT	5 cut(s)	327, 411, 428, 571, 614
MlyI	GAGTC	5 cut(s)	204, 223, 976, 1038, 1074
MmeI	TCCRAC	1 cut(s)	484
MroXI	GAANNNNTTC	1 cut(s)	266
MseI	TTAA	1 cut(s)	489
MslI	CAYNNNNRTG	1 cut(s)	23
MspI	CCGG	3 cut(s)	11, 347, 731
MspR9I	CCNGG	4 cut(s)	75, 348, 731, 921
MvaI	CCWGG	2 cut(s)	75, 921
MvnI	CGCG	1 cut(s)	117
MwoI	GCNNNNNNNGC	2 cut(s)	377, 1004
NciI	CCSGG	2 cut(s)	348, 731
NcoI	CCATGG	1 cut(s)	687
NdeII	GATC	9 cut(s)	87, 169, 174, 530, 576, 586, 712, 828, 1069
NlaIII	CATG	7 cut(s)	280, 436, 672, 691, 784, 916, 1055
NlaIV	GGNNCC	1 cut(s)	1040
NmuCI	GTSAC	2 cut(s)	619, 664
NspV	TTCGAA	1 cut(s)	1151
PagI	TCATGA	2 cut(s)	432, 912
PceI	AGGCCT	1 cut(s)	1133
PciSI	GCTCTTC	1 cut(s)	897
PcsI	WCGNNNNNNNCGW	1 cut(s)	894
PdmI	GAANNNNTTC	1 cut(s)	266
PfoI	TCCNGGA	1 cut(s)	346
PinAI	ACCGGT	1 cut(s)	10
PkrI	GCNGC	8 cut(s)	313, 382, 400, 403, 702, 815, 841, 1009
PleI	GAGTC	5 cut(s)	203, 223, 976, 1037, 1073
PpsI	GAGTC	5 cut(s)	203, 223, 976, 1037, 1073
PpuMI	RGGWCCY	1 cut(s)	821
PsiI	TTATAA	1 cut(s)	291
Psp1406I	AACGTT	1 cut(s)	297
Psp5II	RGGWCCY	1 cut(s)	821
Psp6I	CCWGG	2 cut(s)	73, 919
PspGI	CCWGG	2 cut(s)	73, 919
PspN4I	GGNNCC	1 cut(s)	1040
PspPI	GGNCC	1 cut(s)	821
PspPPI	RGGWCCY	1 cut(s)	821
PstI	CTGCAG	1 cut(s)	815
PsuI	RGATCY	1 cut(s)	530
RsaI	GTAC	2 cut(s)	449, 1040
RsaNI	GTAC	2 cut(s)	448, 1039
RseI	CAYNNNNRTG	1 cut(s)	23
SapI	GCTCTTC	1 cut(s)	897
SaqAI	TTAA	1 cut(s)	489
SatI	GCNGC	8 cut(s)	312, 381, 399, 402, 701, 814, 840, 1008
Sau3AI	GATC	9 cut(s)	87, 169, 174, 530, 576, 586, 712, 828, 1069
Sau96I	GGNCC	1 cut(s)	821
SchI	GAGTC	5 cut(s)	204, 223, 976, 1038, 1074
ScrFI	CCNGG	4 cut(s)	75, 348, 731, 921
SfaNI	GCATC	2 cut(s)	60, 1125
SfcI	CTRYAG	2 cut(s)	153, 811
SfuI	TTCGAA	1 cut(s)	1151
SinI	GGWCC	1 cut(s)	821
SmiMI	CAYNNNNRTG	1 cut(s)	23
SmlI	CTYRAG	1 cut(s)	608
SmoI	CTYRAG	1 cut(s)	608
Sse9I	AATT	5 cut(s)	327, 411, 428, 571, 614
SseBI	AGGCCT	1 cut(s)	1133
SsiI	CCGC	1 cut(s)	1097
SspMI	CTAG	2 cut(s)	377, 543
StuI	AGGCCT	1 cut(s)	1133
StyD4I	CCNGG	4 cut(s)	73, 346, 729, 919
StyI	CCWWGG	3 cut(s)	106, 393, 687
TaaI	ACNGT	2 cut(s)	502, 636
TaiI	ACGT	3 cut(s)	300, 526, 1020
TaqI	TCGA	6 cut(s)	831, 849, 888, 897, 1068, 1151
TaqII	GACCGA	1 cut(s)	191
TasI	AATT	5 cut(s)	327, 411, 428, 571, 614
Tru1I	TTAA	1 cut(s)	489
Tru9I	TTAA	1 cut(s)	489
TseFI	GTSAC	2 cut(s)	619, 664
TseI	GCWGC	8 cut(s)	311, 380, 398, 401, 700, 813, 839, 1007
Tsp45I	GTSAC	2 cut(s)	619, 664
TspDTI	ATGAA	5 cut(s)	421, 584, 800, 901, 929
TspGWI	ACGGA	1 cut(s)	227
VpaK11BI	GGWCC	1 cut(s)	821
XapI	RAATTY	3 cut(s)	428, 571, 614
XmiI	GTMKAC	1 cut(s)	102
XmnI	GAANNNNTTC	1 cut(s)	266
XspI	CTAG	2 cut(s)	377, 543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.