Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005731

Physical Location & Seq

Genomic Coordinates Reverse (-)

24471991 .. 24475932

Gene Structure 3942 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1122100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGGCAAAGCCAGTTTCAATTGAAGTGTGGAATCCCAATGGAAAATACAGAGTTGTCAGCACAAAACCCATGCCTGGAACTCGCTGGATCAATCTCTTAATTGAGCAAGATTGCAGAGTCGAAATATGCACCCAGAAGAAAACAATTCTTTCTGTTGAGGATATCATTGCTCTGATTGGAGATAAGTGTGATGGAGTTATTGGGCAGTTGACAGAAGACTGGGGAGAGACTTTGTTCTCAGCACTGAGCAGAGCAGGAGGCAAAGCCTTCAGCAACATGGCTGTTGGCTACAACAATGTGGATGTTGATGCTGCTAACAAGTATGGAGTTGCTGTTGGGAACACACCTGGAGTACTTACAGAAACTACAGCAGAGCTGGCCGCTTCACTTTCTTTAACTGCCGCTAGAAGAATAGTTGAAGCGGATGAGTTTATGAGGGCCGGCTTATACGATGGATGGCTTCCTAATCTATTTGTGGGAAACTTGCTCAAAGGGCAAACTGTCGGTGTGATTGGAGCTGGCCGTATAGGTTCTGCATATGCCAGAATGATGGTTGAAGGTTTCAAGATGAACCTAATTTACTATGATCTGTACCAGGCTACCCGGCTAGAAAAGTTTGTTACAGCTTACGGTCAGTTCCTAAAAGCTAATGGTGAACAGCCAGTGACTTGGAAAAGAGCATCAAGCATGGAGGAGGTGCTCCGAGAAGCAGATGTGATAAGCCTTCACCCAATTCTGGATAAAACCACTTATCATCTAGTCAACAAAGACAGTCTGGCAATTATGAAGAAGGAAGCAGTCCTTATAAACTGTAGCAGAGGGCCTGTGATTGATGAAGTGGCTCTTGTAGAACATTTGAAACAAAACCCTATGTTTCGAGTTGGCCTGGATGTCTTTGAGGATGAGCCTTACATGAAACCTGGTCTTGCTGACTTGAAGAATGCTGTTGTGGTACCTCACATTGCTTCTGCTTCGAAGTGGACTCGTGAAGGAATGGCAACACTAGCTGCCCTCAATGTCCTGGGAAAAATTAAAGGATACCCTGTTTGGAAGAATCCAAACCAGGTTGAACCATTTCTCAACGAGAATGCTCCGCCACCTGCTGCCAGTCCTAGCATTGTCAACTCAAAGGCCTTAGGTTTACCAGTTTCAAGGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0008465 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009536 GO:0009605 GO:0009628 GO:0009853 GO:0009854 GO:0009987 GO:0009991 GO:0010035 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0030267 GO:0031668 GO:0033554 GO:0042221 GO:0042579 GO:0042631 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050896 GO:0051716 GO:0055114 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071478 GO:0071482 GO:0071496 GO:0097159 GO:0104004 GO:1901363 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

387

Amino Acids

42.17

Weight (kDa)

6.92

Isoelectric Point (pI)

33.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	34 - 347	1.2e-17	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	130 - 322	3.9e-44	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68010 AT1G68010 AT1G68010
fragaria_vesca	FvH4_5g30340 FvH4_5g30340
malus_domestica	MD05G1122100.v1.1
prunus_persica	Prupe.7G264000_v2.0.a1 Prupe.8G166000_v2.0.a1 Prupe.8G166000_v2.0.a1
pyrus_communis	pycom05g11630 pycom10g10740
rosa_chinensis	RchiOBHm_Chr7g0224721 RchiOBHm_Chr7g0224761
rosa_laevigata	RLG00000001858 RLG00000001862
rosa_multiflora	Rmu_sc0000144.1_g000017 Rmu_sc0000144.1_g000025
rosa_roxburghii	Rroxscaffold_3G00234460
rosa_rugosa	Rorug07G0225200 Rorug07G0225400
rosa_samantha	Rh7AG377600 Rh7AG377700 Rh7BG361200 Rh7BG361500 Rh7CG396400 Rh7CG396600 Rh7DG371300
rosa_wichuraiana	Rw7G031340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	806
AarI	CACCTGC	1 cut(s)	1108
Acc36I	ACCTGC	1 cut(s)	1108
Acc65I	GGTACC	1 cut(s)	952
AccB1I	GGYRCC	1 cut(s)	952
AciI	CCGC	4 cut(s)	381, 402, 422, 1094
AclWI	GGATC	1 cut(s)	95
AcoI	YGGCCR	2 cut(s)	378, 520
AcuI	CTGAAG	1 cut(s)	253
AfaI	GTAC	3 cut(s)	354, 593, 954
AfiI	CCNNNNNNNGG	2 cut(s)	74, 736
AgsI	TTSAA	9 cut(s)	18, 23, 419, 557, 565, 859, 937, 1070, 1152
AjnI	CCWGG	7 cut(s)	73, 346, 594, 885, 919, 1020, 1062
AluBI	AGCT	5 cut(s)	376, 518, 626, 647, 1007
AluI	AGCT	5 cut(s)	376, 518, 626, 647, 1007
Alw21I	GWGCWC	1 cut(s)	702
Alw26I	GTCTC	1 cut(s)	221
AlwI	GGATC	1 cut(s)	95
AoxI	GGCC	6 cut(s)	378, 438, 520, 821, 883, 1131
ApeKI	GCWGC	3 cut(s)	311, 1007, 1103
Asp700I	GAANNNNTTC	1 cut(s)	1074
Asp718I	GGTACC	1 cut(s)	952
AspS9I	GGNCC	2 cut(s)	438, 821
AsuC2I	CCSGG	1 cut(s)	604
AsuHPI	GGTGA	2 cut(s)	665, 719
AsuII	TTCGAA	1 cut(s)	974
AxyI	CCTNAGG	1 cut(s)	1135
BanI	GGYRCC	1 cut(s)	952
BauI	CACGAG	1 cut(s)	984
BbsI	GAAGAC	1 cut(s)	222
Bbv12I	GWGCWC	1 cut(s)	702
BbvI	GCAGC	3 cut(s)	298, 994, 1090
BccI	CCATC	4 cut(s)	185, 446, 450, 544
BceAI	ACGGC	1 cut(s)	507
BciT130I	CCWGG	7 cut(s)	75, 348, 596, 887, 921, 1022, 1064
BciVI	GTATCC	1 cut(s)	1031
BcnI	CCSGG	1 cut(s)	604
BcoDI	GTCTC	1 cut(s)	221
BfaI	CTAG	5 cut(s)	405, 608, 758, 1004, 1113
BfmI	CTRYAG	2 cut(s)	366, 811
BfuAI	ACCTGC	1 cut(s)	1108
BfuI	GTATCC	1 cut(s)	1031
BisI	GCNGC	5 cut(s)	312, 381, 402, 1008, 1104
BlsI	GCNGC	5 cut(s)	313, 382, 403, 1009, 1105
BmcAI	AGTACT	1 cut(s)	354
Bme1390I	CCNGG	8 cut(s)	75, 348, 596, 604, 887, 921, 1022, 1064
BmgT120I	GGNCC	2 cut(s)	438, 821
BmiI	GGNNCC	1 cut(s)	954
BmrFI	CCNGG	8 cut(s)	75, 348, 596, 604, 887, 921, 1022, 1064
BmrI	ACTGGG	1 cut(s)	229
BmsI	GCATC	2 cut(s)	298, 689
BmuI	ACTGGG	1 cut(s)	229
BpiI	GAAGAC	1 cut(s)	222
BpmI	CTGGAG	1 cut(s)	369
Bpu14I	TTCGAA	1 cut(s)	974
BpuMI	CCSGG	1 cut(s)	604
BsaJI	CCNNGG	1 cut(s)	1021
BsaXI	ACNNNNNCTCC	2 cut(s)	318, 348
Bsc4I	CCNNNNNNNGG	2 cut(s)	74, 736
Bse118I	RCCGGY	1 cut(s)	440
Bse1I	ACTGG	5 cut(s)	11, 224, 662, 1107, 1145
Bse21I	CCTNAGG	1 cut(s)	1135
Bse3DI	GCAATG	2 cut(s)	165, 960
BseBI	CCWGG	7 cut(s)	75, 348, 596, 887, 921, 1022, 1064
BseDI	CCNNGG	1 cut(s)	1021
BseGI	GGATG	5 cut(s)	307, 430, 461, 895, 907
BseLI	CCNNNNNNNGG	2 cut(s)	74, 736
BseMI	GCAATG	2 cut(s)	165, 960
BseMII	CTCAG	2 cut(s)	236, 252
BseNI	ACTGG	5 cut(s)	11, 224, 662, 1107, 1145
BseRI	GAGGAG	1 cut(s)	707
BseXI	GCAGC	3 cut(s)	298, 994, 1090
BshFI	GGCC	6 cut(s)	380, 440, 522, 823, 885, 1133
BshNI	GGYRCC	1 cut(s)	952
BsiHKAI	GWGCWC	1 cut(s)	702
BsiSI	CCGG	2 cut(s)	441, 604
BslI	CCNNNNNNNGG	2 cut(s)	74, 736
BsmAI	GTCTC	1 cut(s)	221
BsmI	GAATGC	2 cut(s)	946, 1093
BsnI	GGCC	6 cut(s)	380, 440, 522, 823, 885, 1133
Bsp119I	TTCGAA	1 cut(s)	974
Bsp1286I	GDGCHC	1 cut(s)	702
Bsp143I	GATC	2 cut(s)	87, 586
BspACI	CCGC	4 cut(s)	381, 402, 422, 1094
BspANI	GGCC	6 cut(s)	380, 440, 522, 823, 885, 1133
BspCNI	CTCAG	2 cut(s)	237, 251
BspLI	GGNNCC	1 cut(s)	954
BspMI	ACCTGC	1 cut(s)	1108
BspPI	GGATC	1 cut(s)	95
BspT104I	TTCGAA	1 cut(s)	974
BspT107I	GGYRCC	1 cut(s)	952
BsrDI	GCAATG	2 cut(s)	165, 960
BsrFI	RCCGGY	1 cut(s)	440
BsrI	ACTGG	5 cut(s)	11, 224, 662, 1107, 1145
BssAI	RCCGGY	1 cut(s)	440
BssECI	CCNNGG	1 cut(s)	1021
BssMI	GATC	2 cut(s)	87, 586
BssSI	CACGAG	1 cut(s)	984
Bst2BI	CACGAG	1 cut(s)	984
Bst2UI	CCWGG	7 cut(s)	75, 348, 596, 887, 921, 1022, 1064
Bst4CI	ACNGT	4 cut(s)	502, 632, 773, 812
BstBI	TTCGAA	1 cut(s)	974
BstC8I	GCNNGC	3 cut(s)	378, 442, 520
BstDEI	CTNAG	3 cut(s)	238, 245, 1135
BstF5I	GGATG	5 cut(s)	307, 430, 461, 895, 907
BstKTI	GATC	2 cut(s)	90, 589
BstMAI	GTCTC	1 cut(s)	221
BstMBI	GATC	2 cut(s)	87, 586
BstMWI	GCNNNNNNNGC	3 cut(s)	377, 493, 1004
BstNI	CCWGG	7 cut(s)	75, 348, 596, 887, 921, 1022, 1064
BstSCI	CCNGG	8 cut(s)	73, 346, 594, 602, 885, 919, 1020, 1062
BstSFI	CTRYAG	2 cut(s)	366, 811
BstV1I	GCAGC	3 cut(s)	298, 994, 1090
BstV2I	GAAGAC	1 cut(s)	222
BstXI	CCANNNNNNTGG	2 cut(s)	550, 669
Bsu36I	CCTNAGG	1 cut(s)	1135
BsuI	GTATCC	1 cut(s)	1031
BsuRI	GGCC	6 cut(s)	380, 440, 522, 823, 885, 1133
BtsCI	GGATG	5 cut(s)	307, 430, 461, 895, 907
BtsIMutI	CAGTG	2 cut(s)	242, 669
BveI	ACCTGC	1 cut(s)	1108
Cac8I	GCNNGC	3 cut(s)	378, 442, 520
Cfr10I	RCCGGY	1 cut(s)	440
Cfr13I	GGNCC	2 cut(s)	438, 821
CsiI	ACCWGGT	2 cut(s)	919, 1062
Csp6I	GTAC	3 cut(s)	353, 592, 953
CviAII	CATG	4 cut(s)	70, 277, 688, 913
CviQI	GTAC	3 cut(s)	353, 592, 953
DdeI	CTNAG	3 cut(s)	238, 245, 1135
DpnI	GATC	2 cut(s)	89, 588
DpnII	GATC	2 cut(s)	87, 586
EaeI	YGGCCR	2 cut(s)	378, 520
EciI	GGCGGA	1 cut(s)	1083
Eco147I	AGGCCT	1 cut(s)	1133
Eco32I	GATATC	1 cut(s)	163
Eco57I	CTGAAG	1 cut(s)	253
Eco81I	CCTNAGG	1 cut(s)	1135
EcoO109I	RGGNCCY	1 cut(s)	821
EcoRII	CCWGG	7 cut(s)	73, 346, 594, 885, 919, 1020, 1062
EcoRV	GATATC	1 cut(s)	163
FaeI	CATG	4 cut(s)	73, 280, 691, 916
FalI	AAGNNNNNCTT	4 cut(s)	786, 818, 828, 860
FatI	CATG	4 cut(s)	69, 276, 687, 912
FauNDI	CATATG	1 cut(s)	538
Fnu4HI	GCNGC	5 cut(s)	312, 381, 402, 1008, 1104
FokI	GGATG	5 cut(s)	314, 437, 468, 902, 914
Fsp4HI	GCNGC	5 cut(s)	312, 381, 402, 1008, 1104
FspBI	CTAG	5 cut(s)	405, 608, 758, 1004, 1113
GluI	GCNGC	5 cut(s)	312, 381, 402, 1008, 1104
GsuI	CTGGAG	1 cut(s)	369
HaeIII	GGCC	6 cut(s)	380, 440, 522, 823, 885, 1133
HapII	CCGG	2 cut(s)	441, 604
Hin1II	CATG	4 cut(s)	73, 280, 691, 916
HincII	GTYRAC	3 cut(s)	210, 763, 1123
HindII	GTYRAC	3 cut(s)	210, 763, 1123
HinfI	GANTC	4 cut(s)	31, 117, 982, 1054
HpaII	CCGG	2 cut(s)	441, 604
HphI	GGTGA	2 cut(s)	665, 719
Hpy166II	GTNNAC	6 cut(s)	210, 656, 763, 981, 1123, 1142
Hpy188I	TCNGA	2 cut(s)	174, 704
Hpy188III	TCNNGA	3 cut(s)	565, 737, 986
Hpy8I	GTNNAC	6 cut(s)	210, 656, 763, 981, 1123, 1142
HpyAV	CCTTC	5 cut(s)	277, 551, 734, 784, 983
HpyCH4III	ACNGT	4 cut(s)	502, 632, 773, 812
HpyCH4V	TGCA	3 cut(s)	114, 129, 536
HpyF10VI	GCNNNNNNNGC	3 cut(s)	377, 493, 1004
HpyF3I	CTNAG	3 cut(s)	238, 245, 1135
Hsp92II	CATG	4 cut(s)	73, 280, 691, 916
KpnI	GGTACC	1 cut(s)	956
KroI	GCCGGC	1 cut(s)	440
KroNI	GCCGGC	1 cut(s)	442
Kzo9I	GATC	2 cut(s)	87, 586
LmnI	GCTCC	3 cut(s)	515, 705, 1096
Lsp1109I	GCAGC	3 cut(s)	298, 994, 1090
LweI	GCATC	2 cut(s)	298, 689
MabI	ACCWGGT	2 cut(s)	919, 1062
MaeI	CTAG	5 cut(s)	405, 608, 758, 1004, 1113
MaeIII	GTNAC	2 cut(s)	619, 664
MalI	GATC	2 cut(s)	89, 588
MboI	GATC	2 cut(s)	87, 586
MboII	GAAGA	6 cut(s)	148, 227, 420, 799, 949, 1063
MfeI	CAATTG	1 cut(s)	18
MhlI	GDGCHC	1 cut(s)	702
MluCI	AATT	7 cut(s)	18, 99, 144, 576, 732, 780, 1029
MlyI	GAGTC	2 cut(s)	126, 976
MnlI	CCTC	9 cut(s)	151, 251, 429, 685, 688, 812, 892, 966, 1022
MroNI	GCCGGC	1 cut(s)	440
MroXI	GAANNNNTTC	1 cut(s)	1074
MseI	TTAA	3 cut(s)	98, 395, 1032
MspI	CCGG	2 cut(s)	441, 604
MspR9I	CCNGG	8 cut(s)	75, 348, 596, 604, 887, 921, 1022, 1064
MunI	CAATTG	1 cut(s)	18
Mva1269I	GAATGC	2 cut(s)	946, 1093
MvaI	CCWGG	7 cut(s)	75, 348, 596, 887, 921, 1022, 1064
MwoI	GCNNNNNNNGC	3 cut(s)	377, 493, 1004
NaeI	GCCGGC	1 cut(s)	442
NciI	CCSGG	1 cut(s)	604
NdeI	CATATG	1 cut(s)	538
NdeII	GATC	2 cut(s)	87, 586
NgoMIV	GCCGGC	1 cut(s)	440
NlaIII	CATG	4 cut(s)	73, 280, 691, 916
NlaIV	GGNNCC	1 cut(s)	954
NmuCI	GTSAC	1 cut(s)	664
NspV	TTCGAA	1 cut(s)	974
PaqCI	CACCTGC	1 cut(s)	1108
PceI	AGGCCT	1 cut(s)	1133
PctI	GAATGC	2 cut(s)	946, 1093
PdiI	GCCGGC	1 cut(s)	442
PdmI	GAANNNNTTC	1 cut(s)	1074
PfeI	GAWTC	2 cut(s)	31, 1054
PkrI	GCNGC	5 cut(s)	313, 382, 403, 1009, 1105
PleI	GAGTC	2 cut(s)	125, 976
PpsI	GAGTC	2 cut(s)	125, 976
PsiI	TTATAA	1 cut(s)	806
Psp6I	CCWGG	7 cut(s)	73, 346, 594, 885, 919, 1020, 1062
PspGI	CCWGG	7 cut(s)	73, 346, 594, 885, 919, 1020, 1062
PspN4I	GGNNCC	1 cut(s)	954
PspPI	GGNCC	2 cut(s)	438, 821
PsrI	GAACNNNNNNTAC	2 cut(s)	563, 595
RsaI	GTAC	3 cut(s)	354, 593, 954
RsaNI	GTAC	3 cut(s)	353, 592, 953
SaqAI	TTAA	3 cut(s)	98, 395, 1032
SatI	GCNGC	5 cut(s)	312, 381, 402, 1008, 1104
Sau3AI	GATC	2 cut(s)	87, 586
Sau96I	GGNCC	2 cut(s)	438, 821
ScaI	AGTACT	1 cut(s)	354
SchI	GAGTC	2 cut(s)	126, 976
ScrFI	CCNGG	8 cut(s)	75, 348, 596, 604, 887, 921, 1022, 1064
SduI	GDGCHC	1 cut(s)	702
SexAI	ACCWGGT	2 cut(s)	919, 1062
SfaNI	GCATC	2 cut(s)	298, 689
SfcI	CTRYAG	2 cut(s)	366, 811
SfuI	TTCGAA	1 cut(s)	974
Sse9I	AATT	7 cut(s)	18, 99, 144, 576, 732, 780, 1029
SseBI	AGGCCT	1 cut(s)	1133
SsiI	CCGC	4 cut(s)	381, 402, 422, 1094
SspMI	CTAG	5 cut(s)	405, 608, 758, 1004, 1113
StuI	AGGCCT	1 cut(s)	1133
StyD4I	CCNGG	8 cut(s)	73, 346, 594, 602, 885, 919, 1020, 1062
TaaI	ACNGT	4 cut(s)	502, 632, 773, 812
TaqI	TCGA	3 cut(s)	120, 877, 974
TasI	AATT	7 cut(s)	18, 99, 144, 576, 732, 780, 1029
TatI	WGTACW	1 cut(s)	352
TauI	GCSGC	2 cut(s)	383, 404
TfiI	GAWTC	2 cut(s)	31, 1054
Tru1I	TTAA	3 cut(s)	98, 395, 1032
Tru9I	TTAA	3 cut(s)	98, 395, 1032
TscAI	CASTG	2 cut(s)	249, 669
TseFI	GTSAC	1 cut(s)	664
TseI	GCWGC	3 cut(s)	311, 1007, 1103
Tsp45I	GTSAC	1 cut(s)	664
TspDTI	ATGAA	4 cut(s)	584, 800, 849, 929
TspRI	CASTG	2 cut(s)	249, 669
XmnI	GAANNNNTTC	1 cut(s)	1074
XspI	CTAG	5 cut(s)	405, 608, 758, 1004, 1113
ZrmI	AGTACT	1 cut(s)	354

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.