Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0005731

Physical Location & Seq

Genomic Coordinates Forward (+)

20851936 .. 20857417

Gene Structure 5482 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00234460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGACCCATCCCAAATATCATCATCCTCTCTGTCTTCAAATGGAACCATTGAGTATTGTAATTCTGTCTTGGCTGCATTTGATCGGGAGCAAGATTTGTTACTTCGTACGTATACTAACAACAATCTCCTCTTGGCGGAACATCTCAACGACAAAGAGGACGATGCTCAAACTAAAAGGAGAGGCGCAGTTCCTGGTCACATAGTTATTTATCGTGATCAAGAAGGCTGCTGCACACAATTTGTTTACGGACTATTTTGCGAGAGGTCCCTCGATTTGCGTGATGGAATTGGTAGGCTCGAATTGTCATCCCTTAAAAAAATCATAGCTGTTTTTCGAATGCTTGCATATGTTTCAATTGAAGTGTACAACCCAAATGGGAAGTACAGAGTTGTTAGCACAAAACCCATGCCTGGAACTCGCTGGATCAATCTCTTAGTTGAACAAGACTGTAGAGTTGAAATATGCACCCAGAAGAAAACCATTCTATCTGTTGAGGATATCATCGCTCTGATTGGTGACAAGTGTGATGGAGTAATTGGGCAGTTGACAGAAGACTGGGGTGAGACTTTGTTCTCAGCACTGAGCAGAGCTGGAGGTAAAGCTTTCAGTAACATGGCTGTTGGGTATAACAATGTTGATGTCAATGCTGCTAACAAGTATGGAGTTGCTGTTGGAAACACTCCTGGAGTACTTACAGAAACTACAGCAGAGCTGGCAGCTTCACTCTCAGTATCAGCTGCTAGGAGAATAGTGGAAGCAGATGAGTTTATGAGGGCTGGTTTATATGATGGATGGCTTCCCAATCTGTTTGTGGGAAACTTGCTAAAAGGGCAGACTGTTGGTGTGATTGGAGCTGGTCGTATAGGGTCTGCATATGCCAGAATGATGTGTGCTTTATTAAGTATGCTCATGGGACTAAAATCTGTGTTGATCTCAACAGCTTATGGTCAGTTCCTACAAGCCAATGGCGAAAGCCCTGTGACTTGGAAAAGAGCAGCAAGCATGGATGAGGTGCTCCGAGAAGCAGATGTGATAAGCCTTCACCCGATTCTTGATAAAACCACTTATCATCTGATCAACAAGGAAAGATTAGCAACAATGAAGAAGGAAGCAGTCCTTGTTAACTGTAGCAGAGGGCCTGTGGTTGATGAGGTTGCTCTTGTGGAGCATTTGAAACAAAATCCCATGTTTCGAGTTGGTCTTGACGTCTTTGAGGATGAGCCTGACATGAAACCTGGGCTTGCCGACCTGAAGAATGCCATCGTGGTACCTCACATTGCTTCTGCTTCCAAGTGGACTCGAGAAGGAATGGCAACGTTGGCTGCTCTTAATGTCCTGGGTAAAATCAAAGGATATCCAGTTTGGTCTGATGCGAACCGGGTAGAACCATTCCTGAATGAGAATGCTCCACCACCAGCTGCAAGTCCTAGCATTGTTAACGCCAAGGCCTTGGCTTTGAGCATTGATGTAAATGGCCACATTCTGAGCCTTCCTGGTGCAAATGGAGCAATTGTTATGCATGGAAACCAGGCTCTAGCGATACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0008465 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009536 GO:0009605 GO:0009628 GO:0009853 GO:0009854 GO:0009987 GO:0009991 GO:0010035 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0030267 GO:0031668 GO:0033554 GO:0042221 GO:0042579 GO:0042631 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050896 GO:0051716 GO:0055114 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071478 GO:0071482 GO:0071496 GO:0097159 GO:0104004 GO:1901363 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

516

Amino Acids

55.94

Weight (kDa)

5.86

Isoelectric Point (pI)

31.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	145 - 454	6.4e-17	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	243 - 428	2.6e-42	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68010 AT1G68010 AT1G68010
fragaria_vesca	FvH4_5g30340 FvH4_5g30340
malus_domestica	MD05G1122100.v1.1
prunus_persica	Prupe.7G264000_v2.0.a1 Prupe.8G166000_v2.0.a1 Prupe.8G166000_v2.0.a1
pyrus_communis	pycom05g11630 pycom10g10740
rosa_chinensis	RchiOBHm_Chr7g0224721 RchiOBHm_Chr7g0224761
rosa_laevigata	RLG00000001858 RLG00000001862
rosa_multiflora	Rmu_sc0000144.1_g000017 Rmu_sc0000144.1_g000025
rosa_roxburghii	Rroxscaffold_3G00234460
rosa_rugosa	Rorug07G0225200 Rorug07G0225400
rosa_samantha	Rh7AG377600 Rh7AG377700 Rh7BG361200 Rh7BG361500 Rh7CG396400 Rh7CG396600 Rh7DG371300
rosa_wichuraiana	Rw7G031340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1212
Acc65I	GGTACC	1 cut(s)	1270
AccB1I	GGYRCC	1 cut(s)	1270
AccI	GTMKAC	1 cut(s)	113
AciI	CCGC	1 cut(s)	137
AclI	AACGTT	1 cut(s)	1319
AclWI	GGATC	1 cut(s)	434
AcoI	YGGCCR	1 cut(s)	1477
AcuI	CTGAAG	1 cut(s)	1274
AcyI	GRCGYC	1 cut(s)	1209
AfaI	GTAC	5 cut(s)	109, 368, 386, 693, 1272
AfiI	CCNNNNNNNGG	2 cut(s)	136, 413
AgsI	TTSAA	6 cut(s)	39, 357, 362, 443, 461, 1177
AjnI	CCWGG	7 cut(s)	193, 412, 685, 1237, 1338, 1495, 1530
AluBI	AGCT	9 cut(s)	329, 593, 605, 715, 722, 740, 857, 944, 1421
AluI	AGCT	9 cut(s)	329, 593, 605, 715, 722, 740, 857, 944, 1421
Alw21I	GWGCWC	1 cut(s)	1020
Alw26I	GTCTC	1 cut(s)	560
AlwI	GGATC	1 cut(s)	434
AlwNI	CAGNNNCTG	1 cut(s)	194
Ama87I	CYCGRG	1 cut(s)	1302
AoxI	GGCC	3 cut(s)	1139, 1449, 1477
ApeKI	GCWGC	9 cut(s)	74, 228, 231, 650, 719, 740, 998, 1325, 1421
Asp718I	GGTACC	1 cut(s)	1270
AspLEI	GCGC	1 cut(s)	188
AspS9I	GGNCC	3 cut(s)	4, 267, 1139
AsuC2I	CCSGG	1 cut(s)	1382
AsuHPI	GGTGA	3 cut(s)	530, 575, 1037
AsuII	TTCGAA	1 cut(s)	337
AvaI	CYCGRG	1 cut(s)	1302
AvaII	GGWCC	2 cut(s)	4, 267
BalI	TGGCCA	1 cut(s)	1479
BanI	GGYRCC	1 cut(s)	1270
BbsI	GAAGAC	2 cut(s)	27, 561
Bbv12I	GWGCWC	1 cut(s)	1020
BbvI	GCAGC	9 cut(s)	61, 215, 218, 637, 727, 731, 1010, 1312, 1408
BccI	CCATC	6 cut(s)	16, 278, 524, 785, 789, 1271
BciT130I	CCWGG	7 cut(s)	195, 414, 687, 1239, 1340, 1497, 1532
BclI	TGATCA	2 cut(s)	217, 1077
BcnI	CCSGG	1 cut(s)	1382
BcoDI	GTCTC	1 cut(s)	560
BfaI	CTAG	3 cut(s)	744, 1431, 1538
BfmI	CTRYAG	3 cut(s)	451, 705, 1129
BisI	GCNGC	9 cut(s)	75, 229, 232, 651, 720, 741, 999, 1326, 1422
BlsI	GCNGC	9 cut(s)	76, 230, 233, 652, 721, 742, 1000, 1327, 1423
BmcAI	AGTACT	1 cut(s)	693
Bme1390I	CCNGG	8 cut(s)	195, 414, 687, 1239, 1340, 1382, 1497, 1532
Bme18I	GGWCC	2 cut(s)	4, 267
BmeT110I	CYCGRG	1 cut(s)	1302
BmgT120I	GGNCC	3 cut(s)	4, 267, 1139
BmiI	GGNNCC	4 cut(s)	6, 46, 269, 1272
BmrFI	CCNGG	8 cut(s)	195, 414, 687, 1239, 1340, 1382, 1497, 1532
BmrI	ACTGGG	1 cut(s)	568
BmsI	GCATC	2 cut(s)	154, 1363
BmuI	ACTGGG	1 cut(s)	568
BpiI	GAAGAC	2 cut(s)	27, 561
BpmI	CTGGAG	2 cut(s)	615, 708
Bpu14I	TTCGAA	1 cut(s)	337
BpuMI	CCSGG	1 cut(s)	1382
BsaAI	YACGTR	1 cut(s)	111
BsaHI	GRCGYC	1 cut(s)	1209
BsaJI	CCNNGG	4 cut(s)	1238, 1339, 1446, 1452
BsaXI	ACNNNNNCTCC	4 cut(s)	657, 687, 1500, 1530
Bsc4I	CCNNNNNNNGG	2 cut(s)	136, 413
Bse1I	ACTGG	2 cut(s)	563, 1361
Bse3DI	GCAATG	1 cut(s)	1278
BseBI	CCWGG	7 cut(s)	195, 414, 687, 1239, 1340, 1497, 1532
BseDI	CCNNGG	4 cut(s)	1238, 1339, 1446, 1452
BseGI	GGATG	6 cut(s)	8, 23, 308, 800, 1015, 1225
BseLI	CCNNNNNNNGG	2 cut(s)	136, 413
BseMI	GCAATG	1 cut(s)	1278
BseMII	CTCAG	4 cut(s)	575, 591, 744, 1478
BseNI	ACTGG	2 cut(s)	563, 1361
BseRI	GAGGAG	1 cut(s)	119
BseXI	GCAGC	9 cut(s)	61, 215, 218, 637, 727, 731, 1010, 1312, 1408
BsgI	GTGCAG	1 cut(s)	217
BshFI	GGCC	3 cut(s)	1141, 1451, 1479
BshNI	GGYRCC	1 cut(s)	1270
BsiHKAI	GWGCWC	1 cut(s)	1020
BsiHKCI	CYCGRG	1 cut(s)	1302
BsiSI	CCGG	1 cut(s)	1381
BsiWI	CGTACG	1 cut(s)	107
BslFI	GGGAC	2 cut(s)	253, 930
BslI	CCNNNNNNNGG	2 cut(s)	136, 413
BsmAI	GTCTC	1 cut(s)	560
BsmFI	GGGAC	2 cut(s)	253, 930
BsmI	GAATGC	3 cut(s)	345, 1264, 1411
BsnI	GGCC	3 cut(s)	1141, 1451, 1479
BsoBI	CYCGRG	1 cut(s)	1302
Bsp119I	TTCGAA	1 cut(s)	337
Bsp1286I	GDGCHC	1 cut(s)	1020
Bsp1407I	TGTACA	1 cut(s)	366
Bsp143I	GATC	5 cut(s)	82, 217, 426, 933, 1077
BspACI	CCGC	1 cut(s)	137
BspANI	GGCC	3 cut(s)	1141, 1451, 1479
BspCNI	CTCAG	4 cut(s)	576, 590, 743, 1479
BspLI	GGNNCC	4 cut(s)	6, 46, 269, 1272
BspPI	GGATC	1 cut(s)	434
BspT104I	TTCGAA	1 cut(s)	337
BspT107I	GGYRCC	1 cut(s)	1270
BsrDI	GCAATG	1 cut(s)	1278
BsrGI	TGTACA	1 cut(s)	366
BsrI	ACTGG	2 cut(s)	563, 1361
BssECI	CCNNGG	4 cut(s)	1238, 1339, 1446, 1452
BssMI	GATC	5 cut(s)	82, 217, 426, 933, 1077
BssNAI	GTATAC	1 cut(s)	114
BssNI	GRCGYC	1 cut(s)	1209
BssT1I	CCWWGG	2 cut(s)	1446, 1452
Bst1107I	GTATAC	1 cut(s)	114
Bst2UI	CCWGG	7 cut(s)	195, 414, 687, 1239, 1340, 1497, 1532
Bst4CI	ACNGT	4 cut(s)	452, 841, 1130, 1548
BstACI	GRCGYC	1 cut(s)	1209
BstAUI	TGTACA	1 cut(s)	366
BstBAI	YACGTR	1 cut(s)	111
BstBI	TTCGAA	1 cut(s)	337
BstC8I	GCNNGC	4 cut(s)	345, 717, 1003, 1245
BstDEI	CTNAG	5 cut(s)	436, 577, 584, 730, 1487
BstF5I	GGATG	6 cut(s)	8, 23, 308, 800, 1015, 1225
BstHHI	GCGC	1 cut(s)	188
BstKTI	GATC	5 cut(s)	85, 220, 429, 936, 1080
BstMAI	GTCTC	1 cut(s)	560
BstMBI	GATC	5 cut(s)	82, 217, 426, 933, 1077
BstMWI	GCNNNNNNNGC	4 cut(s)	716, 832, 1322, 1508
BstNI	CCWGG	7 cut(s)	195, 414, 687, 1239, 1340, 1497, 1532
BstSCI	CCNGG	8 cut(s)	193, 412, 685, 1237, 1338, 1380, 1495, 1530
BstSFI	CTRYAG	3 cut(s)	451, 705, 1129
BstSNI	TACGTA	1 cut(s)	111
BstV1I	GCAGC	9 cut(s)	61, 215, 218, 637, 727, 731, 1010, 1312, 1408
BstV2I	GAAGAC	2 cut(s)	27, 561
BstXI	CCANNNNNNTGG	1 cut(s)	1453
BstZ17I	GTATAC	1 cut(s)	114
BsuRI	GGCC	3 cut(s)	1141, 1451, 1479
BtgZI	GCGATG	1 cut(s)	490
BtsCI	GGATG	6 cut(s)	8, 23, 308, 800, 1015, 1225
BtsIMutI	CAGTG	1 cut(s)	581
Cac8I	GCNNGC	4 cut(s)	345, 717, 1003, 1245
CaiI	CAGNNNCTG	1 cut(s)	194
CfoI	GCGC	1 cut(s)	188
Cfr13I	GGNCC	3 cut(s)	4, 267, 1139
Csp6I	GTAC	5 cut(s)	108, 367, 385, 692, 1271
CviAII	CATG	7 cut(s)	409, 616, 913, 1006, 1189, 1231, 1523
CviQI	GTAC	5 cut(s)	108, 367, 385, 692, 1271
DdeI	CTNAG	5 cut(s)	436, 577, 584, 730, 1487
DpnI	GATC	5 cut(s)	84, 219, 428, 935, 1079
DpnII	GATC	5 cut(s)	82, 217, 426, 933, 1077
EaeI	YGGCCR	1 cut(s)	1477
EciI	GGCGGA	1 cut(s)	152
Eco105I	TACGTA	1 cut(s)	111
Eco130I	CCWWGG	2 cut(s)	1446, 1452
Eco147I	AGGCCT	1 cut(s)	1451
Eco32I	GATATC	2 cut(s)	502, 1358
Eco47I	GGWCC	2 cut(s)	4, 267
Eco57I	CTGAAG	1 cut(s)	1274
Eco88I	CYCGRG	1 cut(s)	1302
EcoO109I	RGGNCCY	2 cut(s)	267, 1139
EcoRII	CCWGG	7 cut(s)	193, 412, 685, 1237, 1338, 1495, 1530
EcoRV	GATATC	2 cut(s)	502, 1358
EcoT14I	CCWWGG	2 cut(s)	1446, 1452
EcoT22I	ATGCAT	1 cut(s)	1524
ErhI	CCWWGG	2 cut(s)	1446, 1452
FaeI	CATG	7 cut(s)	412, 619, 916, 1009, 1192, 1234, 1526
FalI	AAGNNNNNCTT	2 cut(s)	1104, 1136
FaqI	GGGAC	2 cut(s)	253, 930
FatI	CATG	7 cut(s)	408, 615, 912, 1005, 1188, 1230, 1522
FauNDI	CATATG	2 cut(s)	349, 877
FbaI	TGATCA	2 cut(s)	217, 1077
FblI	GTMKAC	1 cut(s)	113
Fnu4HI	GCNGC	9 cut(s)	75, 229, 232, 651, 720, 741, 999, 1326, 1422
FokI	GGATG	5 cut(s)	10, 295, 807, 1022, 1232
Fsp4HI	GCNGC	9 cut(s)	75, 229, 232, 651, 720, 741, 999, 1326, 1422
FspBI	CTAG	3 cut(s)	744, 1431, 1538
GlaI	GCGC	1 cut(s)	187
GluI	GCNGC	9 cut(s)	75, 229, 232, 651, 720, 741, 999, 1326, 1422
GsuI	CTGGAG	2 cut(s)	615, 708
HaeIII	GGCC	3 cut(s)	1141, 1451, 1479
HapII	CCGG	1 cut(s)	1381
HhaI	GCGC	1 cut(s)	188
Hin1I	GRCGYC	1 cut(s)	1209
Hin1II	CATG	7 cut(s)	412, 619, 916, 1009, 1192, 1234, 1526
Hin6I	GCGC	1 cut(s)	186
HinP1I	GCGC	1 cut(s)	186
HincII	GTYRAC	3 cut(s)	549, 1126, 1441
HindII	GTYRAC	3 cut(s)	549, 1126, 1441
HindIII	AAGCTT	1 cut(s)	603
HinfI	GANTC	2 cut(s)	1051, 1300
HpaI	GTTAAC	2 cut(s)	1126, 1441
HpaII	CCGG	1 cut(s)	1381
HphI	GGTGA	3 cut(s)	530, 575, 1037
Hpy166II	GTNNAC	7 cut(s)	114, 247, 367, 549, 1126, 1299, 1441
Hpy188I	TCNGA	5 cut(s)	513, 1022, 1077, 1372, 1488
Hpy188III	TCNNGA	7 cut(s)	86, 215, 221, 1055, 1205, 1304, 1396
Hpy8I	GTNNAC	7 cut(s)	114, 247, 367, 549, 1126, 1299, 1441
HpyAV	CCTTC	5 cut(s)	218, 1052, 1102, 1301, 1502
HpyCH4III	ACNGT	4 cut(s)	452, 841, 1130, 1548
HpyCH4IV	ACGT	3 cut(s)	110, 1209, 1319
HpyCH4V	TGCA	8 cut(s)	77, 234, 347, 468, 875, 1424, 1502, 1522
HpyF10VI	GCNNNNNNNGC	4 cut(s)	716, 832, 1322, 1508
HpyF3I	CTNAG	5 cut(s)	436, 577, 584, 730, 1487
HpySE526I	ACGT	3 cut(s)	110, 1209, 1319
Hsp92I	GRCGYC	1 cut(s)	1209
Hsp92II	CATG	7 cut(s)	412, 619, 916, 1009, 1192, 1234, 1526
HspAI	GCGC	1 cut(s)	186
KpnI	GGTACC	1 cut(s)	1274
Ksp22I	TGATCA	2 cut(s)	217, 1077
KspAI	GTTAAC	2 cut(s)	1126, 1441
Kzo9I	GATC	5 cut(s)	82, 217, 426, 933, 1077
LmnI	GCTCC	6 cut(s)	88, 854, 1023, 1168, 1414, 1508
Lsp1109I	GCAGC	9 cut(s)	61, 215, 218, 637, 727, 731, 1010, 1312, 1408
LweI	GCATC	2 cut(s)	154, 1363
MaeI	CTAG	3 cut(s)	744, 1431, 1538
MaeII	ACGT	3 cut(s)	110, 1209, 1319
MaeIII	GTNAC	5 cut(s)	99, 197, 518, 611, 982
MalI	GATC	5 cut(s)	84, 219, 428, 935, 1079
MboI	GATC	5 cut(s)	82, 217, 426, 933, 1077
MboII	GAAGA	5 cut(s)	27, 487, 566, 1117, 1267
MfeI	CAATTG	2 cut(s)	357, 1512
MhlI	GDGCHC	1 cut(s)	1020
MlsI	TGGCCA	1 cut(s)	1479
MluCI	AATT	7 cut(s)	61, 239, 288, 302, 357, 537, 1512
MluNI	TGGCCA	1 cut(s)	1479
MlyI	GAGTC	1 cut(s)	1294
MmeI	TCCRAC	1 cut(s)	655
Mox20I	TGGCCA	1 cut(s)	1479
Mph1103I	ATGCAT	1 cut(s)	1524
MscI	TGGCCA	1 cut(s)	1479
MseI	TTAA	5 cut(s)	315, 902, 1125, 1332, 1440
Msp20I	TGGCCA	1 cut(s)	1479
MspA1I	CMGCKG	2 cut(s)	740, 1421
MspI	CCGG	1 cut(s)	1381
MspR9I	CCNGG	8 cut(s)	195, 414, 687, 1239, 1340, 1382, 1497, 1532
MunI	CAATTG	2 cut(s)	357, 1512
Mva1269I	GAATGC	3 cut(s)	345, 1264, 1411
MvaI	CCWGG	7 cut(s)	195, 414, 687, 1239, 1340, 1497, 1532
MwoI	GCNNNNNNNGC	4 cut(s)	716, 832, 1322, 1508
NciI	CCSGG	1 cut(s)	1382
NdeI	CATATG	2 cut(s)	349, 877
NdeII	GATC	5 cut(s)	82, 217, 426, 933, 1077
NlaIII	CATG	7 cut(s)	412, 619, 916, 1009, 1192, 1234, 1526
NlaIV	GGNNCC	4 cut(s)	6, 46, 269, 1272
NmuCI	GTSAC	3 cut(s)	197, 518, 982
NsiI	ATGCAT	1 cut(s)	1524
NspV	TTCGAA	1 cut(s)	337
PaeR7I	CTCGAG	1 cut(s)	1302
PceI	AGGCCT	1 cut(s)	1451
PctI	GAATGC	3 cut(s)	345, 1264, 1411
PfeI	GAWTC	1 cut(s)	1051
Pfl23II	CGTACG	1 cut(s)	107
PfoI	TCCNGGA	1 cut(s)	685
PkrI	GCNGC	9 cut(s)	76, 230, 233, 652, 721, 742, 1000, 1327, 1423
PleI	GAGTC	1 cut(s)	1294
PpsI	GAGTC	1 cut(s)	1294
Ppu21I	YACGTR	1 cut(s)	111
PpuMI	RGGWCCY	1 cut(s)	267
Psp1406I	AACGTT	1 cut(s)	1319
Psp5II	RGGWCCY	1 cut(s)	267
Psp6I	CCWGG	7 cut(s)	193, 412, 685, 1237, 1338, 1495, 1530
PspGI	CCWGG	7 cut(s)	193, 412, 685, 1237, 1338, 1495, 1530
PspLI	CGTACG	1 cut(s)	107
PspN4I	GGNNCC	4 cut(s)	6, 46, 269, 1272
PspPI	GGNCC	3 cut(s)	4, 267, 1139
PspPPI	RGGWCCY	1 cut(s)	267
PstNI	CAGNNNCTG	1 cut(s)	194
PvuII	CAGCTG	2 cut(s)	740, 1421
RsaI	GTAC	5 cut(s)	109, 368, 386, 693, 1272
RsaNI	GTAC	5 cut(s)	108, 367, 385, 692, 1271
SaqAI	TTAA	5 cut(s)	315, 902, 1125, 1332, 1440
SatI	GCNGC	9 cut(s)	75, 229, 232, 651, 720, 741, 999, 1326, 1422
Sau3AI	GATC	5 cut(s)	82, 217, 426, 933, 1077
Sau96I	GGNCC	3 cut(s)	4, 267, 1139
ScaI	AGTACT	1 cut(s)	693
SchI	GAGTC	1 cut(s)	1294
ScrFI	CCNGG	8 cut(s)	195, 414, 687, 1239, 1340, 1382, 1497, 1532
SduI	GDGCHC	1 cut(s)	1020
SfaNI	GCATC	2 cut(s)	154, 1363
SfcI	CTRYAG	3 cut(s)	451, 705, 1129
Sfr274I	CTCGAG	1 cut(s)	1302
SfuI	TTCGAA	1 cut(s)	337
SinI	GGWCC	2 cut(s)	4, 267
SlaI	CTCGAG	1 cut(s)	1302
SmlI	CTYRAG	1 cut(s)	1302
SmoI	CTYRAG	1 cut(s)	1302
SnaBI	TACGTA	1 cut(s)	111
Sse9I	AATT	7 cut(s)	61, 239, 288, 302, 357, 537, 1512
SseBI	AGGCCT	1 cut(s)	1451
SsiI	CCGC	1 cut(s)	137
SspMI	CTAG	3 cut(s)	744, 1431, 1538
StuI	AGGCCT	1 cut(s)	1451
StyD4I	CCNGG	8 cut(s)	193, 412, 685, 1237, 1338, 1380, 1495, 1530
StyI	CCWWGG	2 cut(s)	1446, 1452
TaaI	ACNGT	4 cut(s)	452, 841, 1130, 1548
TaiI	ACGT	3 cut(s)	113, 1212, 1322
TaqI	TCGA	5 cut(s)	273, 300, 337, 1195, 1303
TasI	AATT	7 cut(s)	61, 239, 288, 302, 357, 537, 1512
TatI	WGTACW	3 cut(s)	366, 384, 691
TfiI	GAWTC	1 cut(s)	1051
Tru1I	TTAA	5 cut(s)	315, 902, 1125, 1332, 1440
Tru9I	TTAA	5 cut(s)	315, 902, 1125, 1332, 1440
TscAI	CASTG	1 cut(s)	588
TseFI	GTSAC	3 cut(s)	197, 518, 982
TseI	GCWGC	9 cut(s)	74, 228, 231, 650, 719, 740, 998, 1325, 1421
Tsp45I	GTSAC	3 cut(s)	197, 518, 982
TspDTI	ATGAA	2 cut(s)	1118, 1247
TspGWI	ACGGA	1 cut(s)	264
TspRI	CASTG	1 cut(s)	588
VpaK11BI	GGWCC	2 cut(s)	4, 267
XcmI	CCANNNNNNNNNTGG	1 cut(s)	811
XhoI	CTCGAG	1 cut(s)	1302
XmiI	GTMKAC	1 cut(s)	113
XspI	CTAG	3 cut(s)	744, 1431, 1538
ZraI	GACGTC	1 cut(s)	1210
ZrmI	AGTACT	1 cut(s)	693
Zsp2I	ATGCAT	1 cut(s)	1524

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.