Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0005731

Physical Location & Seq

Genomic Coordinates Forward (+)

22153167 .. 22156407

Gene Structure 3241 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001858

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGGCCAAACCAGTTTCAATTGAAGTGTACAACCCAAATGGGAAGTACAGAGTTGTTAGCACAAAACCCATGCCTGGAACTCGCTGGATCAATCTCTTAGTTGAACAAGACTGTAGAGTTGAAATATGCACCCAGAAGAAAACCATTCTATCTGTTGAGGATATCATCGCTCTGATTGGTGACAAGTGTGATGGAGTAATTGGGCAGTTGACAGAAGACTGGGGTGAGACTTTGTTCTCAGCACTGAGCAGAGCTGGAGGTAAAGCTTTCAGCAACATGGCTGTTGGGTATAACAATGTTGATGTCAATGCTGCTAACAAGTATGGAGTTGCTGTTGGAAACACTCCTGGAGTACTTACAGAAACTACAGCAGAGCTGGCAGCTTCACTCTCAGTATCAGCTGCTAGGAGAATAGTGGAAGCAGATGAGTTTATGAGGGCTGGTTTATATGATGGATGGCTTCCCAATCTGTTTGTGGGAAACTTGCTAAAAGGGCAGACTGTTGGTGTGATTGGAGCTGGTCGTATAGGGTCTGCATATGCCAGAATGATGGTTGAAGGTTTCAAGATGAACCTAATTTACTATGATCTGTACCAGGCTACACGACTAGAAAAGTTTGTTACAGCTTATGGTCAGTTCCTACAAGCCAATGGTGAAAGCCCTGTGACTTGGAAAAGAGCAGCAAGCATGGATGAGGTGCTCCGAGAAGCAGATGTGATAAGCCTTCACCCAATTCTCGATAAAACCACTTATCATCTGATCAACAAAGAAAGATTAGCGACGATGAAGAAGGAAGCAGTCCTTGTTAACTGTAGCAGAGGGCCTGTGATTGATGAGGTTGCTCTTGTGGAGCATTTGAAACAAAATCCCATGTTTCGAGTTGGTCTTGACGTCTTTGAGGATGAGCCTTACATGAAACCTGGGCTTGCCGACCTGAAGAATGCCATCGTGGTACCTCACATTGCTTCTGCCTCCAAGTGGACTCGAGAAGGAATGGCAACATTGGCTGCTCTTAATGTCCTGGGTAAAATCAAAGGATATCCAATTTGGTCTGATGCGAACCGGGTAGAACCATTCCTGAATGAGAATGCTTCACCACCAGCTGCCAGTCCTAGCATTGTTAACGCCAAGGCCTTGGGTAATAATATCTATAAAACTATCAATTACACGCACAGACGTCGAATTTCTTTTCTGAAAACTCTGTTTCACTGCAGGATTACCAGTTTCAAGGCTTTGAGCATTGATGTAAATGGCCACATTTTGAGCCTTCCTGGTGCAAATGGAGCAATTGTTATGCATGGAAACCAGGCTCTGTCGATACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0008465 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009536 GO:0009605 GO:0009628 GO:0009853 GO:0009854 GO:0009987 GO:0009991 GO:0010035 GO:0016491 GO:0016614 GO:0016616 GO:0016618 GO:0030267 GO:0031668 GO:0033554 GO:0042221 GO:0042579 GO:0042631 GO:0043094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050896 GO:0051716 GO:0055114 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071478 GO:0071482 GO:0071496 GO:0097159 GO:0104004 GO:1901363 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

442

Amino Acids

48.23

Weight (kDa)

8.76

Isoelectric Point (pI)

32.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	32 - 347	4.6e-17	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	130 - 322	9.4e-45	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005731)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68010 AT1G68010 AT1G68010
fragaria_vesca	FvH4_5g30340 FvH4_5g30340
malus_domestica	MD05G1122100.v1.1
prunus_persica	Prupe.7G264000_v2.0.a1 Prupe.8G166000_v2.0.a1 Prupe.8G166000_v2.0.a1
pyrus_communis	pycom05g11630 pycom10g10740
rosa_chinensis	RchiOBHm_Chr7g0224721 RchiOBHm_Chr7g0224761
rosa_laevigata	RLG00000001858 RLG00000001862
rosa_multiflora	Rmu_sc0000144.1_g000017 Rmu_sc0000144.1_g000025
rosa_roxburghii	Rroxscaffold_3G00234460
rosa_rugosa	Rorug07G0225200 Rorug07G0225400
rosa_samantha	Rh7AG377600 Rh7AG377700 Rh7BG361200 Rh7BG361500 Rh7CG396400 Rh7CG396600 Rh7DG371300
rosa_wichuraiana	Rw7G031340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	894, 1180
Acc65I	GGTACC	1 cut(s)	952
AccB1I	GGYRCC	1 cut(s)	952
AclWI	GGATC	1 cut(s)	95
AcoI	YGGCCR	2 cut(s)	3, 1252
AcsI	RAATTY	1 cut(s)	1182
AcuI	CTGAAG	1 cut(s)	956
AcyI	GRCGYC	2 cut(s)	891, 1177
AfaI	GTAC	5 cut(s)	29, 47, 354, 593, 954
AfiI	CCNNNNNNNGG	2 cut(s)	74, 978
AgsI	TTSAA	8 cut(s)	18, 23, 104, 122, 557, 565, 859, 1228
AjnI	CCWGG	7 cut(s)	73, 346, 594, 919, 1020, 1270, 1305
AluBI	AGCT	8 cut(s)	254, 266, 376, 383, 401, 518, 626, 1103
AluI	AGCT	8 cut(s)	254, 266, 376, 383, 401, 518, 626, 1103
Alw21I	GWGCWC	1 cut(s)	702
Alw26I	GTCTC	1 cut(s)	221
AlwI	GGATC	1 cut(s)	95
AlwNI	CAGNNNCTG	1 cut(s)	1312
Ama87I	CYCGRG	1 cut(s)	984
AoxI	GGCC	4 cut(s)	3, 821, 1131, 1252
ApeKI	GCWGC	6 cut(s)	311, 380, 401, 680, 1007, 1103
ApoI	RAATTY	1 cut(s)	1182
Asp718I	GGTACC	1 cut(s)	952
AspS9I	GGNCC	1 cut(s)	821
AsuC2I	CCSGG	1 cut(s)	1064
AsuHPI	GGTGA	5 cut(s)	191, 236, 665, 719, 1086
AvaI	CYCGRG	1 cut(s)	984
BalI	TGGCCA	2 cut(s)	5, 1254
BanI	GGYRCC	1 cut(s)	952
BbsI	GAAGAC	1 cut(s)	222
Bbv12I	GWGCWC	1 cut(s)	702
BbvI	GCAGC	6 cut(s)	298, 388, 392, 692, 994, 1090
BccI	CCATC	5 cut(s)	185, 446, 450, 544, 953
BcgI	CGANNNNNNTGC	2 cut(s)	1160, 1194
BciT130I	CCWGG	7 cut(s)	75, 348, 596, 921, 1022, 1272, 1307
BclI	TGATCA	1 cut(s)	759
BcnI	CCSGG	1 cut(s)	1064
BcoDI	GTCTC	1 cut(s)	221
BfaI	CTAG	3 cut(s)	405, 608, 1113
BfmI	CTRYAG	4 cut(s)	112, 366, 811, 1210
BisI	GCNGC	6 cut(s)	312, 381, 402, 681, 1008, 1104
BlsI	GCNGC	6 cut(s)	313, 382, 403, 682, 1009, 1105
BmcAI	AGTACT	1 cut(s)	354
Bme1390I	CCNGG	8 cut(s)	75, 348, 596, 921, 1022, 1064, 1272, 1307
BmeT110I	CYCGRG	1 cut(s)	984
BmgT120I	GGNCC	1 cut(s)	821
BmiI	GGNNCC	1 cut(s)	954
BmrFI	CCNGG	8 cut(s)	75, 348, 596, 921, 1022, 1064, 1272, 1307
BmrI	ACTGGG	1 cut(s)	229
BmsI	GCATC	1 cut(s)	1045
BmuI	ACTGGG	1 cut(s)	229
BpiI	GAAGAC	1 cut(s)	222
BpmI	CTGGAG	2 cut(s)	276, 369
BpuMI	CCSGG	1 cut(s)	1064
BsaHI	GRCGYC	2 cut(s)	891, 1177
BsaJI	CCNNGG	4 cut(s)	920, 1021, 1128, 1134
BsaXI	ACNNNNNCTCC	4 cut(s)	318, 348, 1275, 1305
Bsc4I	CCNNNNNNNGG	2 cut(s)	74, 978
Bse1I	ACTGG	4 cut(s)	11, 224, 1107, 1221
Bse3DI	GCAATG	1 cut(s)	960
BseBI	CCWGG	7 cut(s)	75, 348, 596, 921, 1022, 1272, 1307
BseDI	CCNNGG	4 cut(s)	920, 1021, 1128, 1134
BseGI	GGATG	3 cut(s)	461, 697, 907
BseLI	CCNNNNNNNGG	2 cut(s)	74, 978
BseMI	GCAATG	1 cut(s)	960
BseMII	CTCAG	3 cut(s)	236, 252, 405
BseNI	ACTGG	4 cut(s)	11, 224, 1107, 1221
BseXI	GCAGC	6 cut(s)	298, 388, 392, 692, 994, 1090
BshFI	GGCC	4 cut(s)	5, 823, 1133, 1254
BshNI	GGYRCC	1 cut(s)	952
BsiHKAI	GWGCWC	1 cut(s)	702
BsiHKCI	CYCGRG	1 cut(s)	984
BsiSI	CCGG	1 cut(s)	1063
BslI	CCNNNNNNNGG	2 cut(s)	74, 978
BsmAI	GTCTC	1 cut(s)	221
BsmI	GAATGC	2 cut(s)	946, 1093
BsnI	GGCC	4 cut(s)	5, 823, 1133, 1254
BsoBI	CYCGRG	1 cut(s)	984
Bsp1286I	GDGCHC	1 cut(s)	702
Bsp1407I	TGTACA	1 cut(s)	27
Bsp143I	GATC	3 cut(s)	87, 586, 759
BspANI	GGCC	4 cut(s)	5, 823, 1133, 1254
BspCNI	CTCAG	3 cut(s)	237, 251, 404
BspLI	GGNNCC	1 cut(s)	954
BspMAI	CTGCAG	1 cut(s)	1214
BspPI	GGATC	1 cut(s)	95
BspT107I	GGYRCC	1 cut(s)	952
BsrDI	GCAATG	1 cut(s)	960
BsrGI	TGTACA	1 cut(s)	27
BsrI	ACTGG	4 cut(s)	11, 224, 1107, 1221
BssECI	CCNNGG	4 cut(s)	920, 1021, 1128, 1134
BssMI	GATC	3 cut(s)	87, 586, 759
BssNI	GRCGYC	2 cut(s)	891, 1177
BssT1I	CCWWGG	2 cut(s)	1128, 1134
Bst2UI	CCWGG	7 cut(s)	75, 348, 596, 921, 1022, 1272, 1307
Bst4CI	ACNGT	4 cut(s)	113, 502, 812, 1323
BstACI	GRCGYC	2 cut(s)	891, 1177
BstAUI	TGTACA	1 cut(s)	27
BstC8I	GCNNGC	3 cut(s)	378, 685, 927
BstDEI	CTNAG	4 cut(s)	97, 238, 245, 391
BstF5I	GGATG	3 cut(s)	461, 697, 907
BstKTI	GATC	3 cut(s)	90, 589, 762
BstMAI	GTCTC	1 cut(s)	221
BstMBI	GATC	3 cut(s)	87, 586, 759
BstMWI	GCNNNNNNNGC	4 cut(s)	377, 493, 1004, 1283
BstNI	CCWGG	7 cut(s)	75, 348, 596, 921, 1022, 1272, 1307
BstSCI	CCNGG	8 cut(s)	73, 346, 594, 919, 1020, 1062, 1270, 1305
BstSFI	CTRYAG	4 cut(s)	112, 366, 811, 1210
BstV1I	GCAGC	6 cut(s)	298, 388, 392, 692, 994, 1090
BstV2I	GAAGAC	1 cut(s)	222
BstXI	CCANNNNNNTGG	2 cut(s)	550, 1135
BsuRI	GGCC	4 cut(s)	5, 823, 1133, 1254
BtgZI	GCGATG	1 cut(s)	151
BtsCI	GGATG	3 cut(s)	461, 697, 907
BtsI	GCAGTG	1 cut(s)	1207
BtsIMutI	CAGTG	2 cut(s)	242, 1207
Cac8I	GCNNGC	3 cut(s)	378, 685, 927
CaiI	CAGNNNCTG	1 cut(s)	1312
Cfr13I	GGNCC	1 cut(s)	821
Csp6I	GTAC	5 cut(s)	28, 46, 353, 592, 953
CviAII	CATG	6 cut(s)	70, 277, 688, 871, 913, 1298
CviQI	GTAC	5 cut(s)	28, 46, 353, 592, 953
DdeI	CTNAG	4 cut(s)	97, 238, 245, 391
DpnI	GATC	3 cut(s)	89, 588, 761
DpnII	GATC	3 cut(s)	87, 586, 759
EaeI	YGGCCR	2 cut(s)	3, 1252
Eco130I	CCWWGG	2 cut(s)	1128, 1134
Eco147I	AGGCCT	1 cut(s)	1133
Eco32I	GATATC	2 cut(s)	163, 1040
Eco57I	CTGAAG	1 cut(s)	956
Eco88I	CYCGRG	1 cut(s)	984
EcoO109I	RGGNCCY	1 cut(s)	821
EcoRII	CCWGG	7 cut(s)	73, 346, 594, 919, 1020, 1270, 1305
EcoRV	GATATC	2 cut(s)	163, 1040
EcoT14I	CCWWGG	2 cut(s)	1128, 1134
EcoT22I	ATGCAT	1 cut(s)	1299
ErhI	CCWWGG	2 cut(s)	1128, 1134
FaeI	CATG	6 cut(s)	73, 280, 691, 874, 916, 1301
FalI	AAGNNNNNCTT	2 cut(s)	786, 818
FatI	CATG	6 cut(s)	69, 276, 687, 870, 912, 1297
FauNDI	CATATG	1 cut(s)	538
FbaI	TGATCA	1 cut(s)	759
Fnu4HI	GCNGC	6 cut(s)	312, 381, 402, 681, 1008, 1104
FokI	GGATG	3 cut(s)	468, 704, 914
Fsp4HI	GCNGC	6 cut(s)	312, 381, 402, 681, 1008, 1104
FspBI	CTAG	3 cut(s)	405, 608, 1113
GluI	GCNGC	6 cut(s)	312, 381, 402, 681, 1008, 1104
GsuI	CTGGAG	2 cut(s)	276, 369
HaeIII	GGCC	4 cut(s)	5, 823, 1133, 1254
HapII	CCGG	1 cut(s)	1063
Hin1I	GRCGYC	2 cut(s)	891, 1177
Hin1II	CATG	6 cut(s)	73, 280, 691, 874, 916, 1301
HincII	GTYRAC	3 cut(s)	210, 808, 1123
HindII	GTYRAC	3 cut(s)	210, 808, 1123
HindIII	AAGCTT	1 cut(s)	264
HinfI	GANTC	1 cut(s)	982
HpaI	GTTAAC	2 cut(s)	808, 1123
HpaII	CCGG	1 cut(s)	1063
HphI	GGTGA	5 cut(s)	191, 236, 665, 719, 1086
Hpy166II	GTNNAC	5 cut(s)	28, 210, 808, 981, 1123
Hpy188I	TCNGA	5 cut(s)	174, 704, 759, 1054, 1194
Hpy188III	TCNNGA	5 cut(s)	565, 737, 887, 986, 1078
Hpy8I	GTNNAC	5 cut(s)	28, 210, 808, 981, 1123
Hpy99I	CGWCG	2 cut(s)	784, 1182
HpyAV	CCTTC	5 cut(s)	551, 734, 784, 983, 1277
HpyCH4III	ACNGT	4 cut(s)	113, 502, 812, 1323
HpyCH4IV	ACGT	2 cut(s)	891, 1177
HpyCH4V	TGCA	5 cut(s)	129, 536, 1212, 1277, 1297
HpyF10VI	GCNNNNNNNGC	4 cut(s)	377, 493, 1004, 1283
HpyF3I	CTNAG	4 cut(s)	97, 238, 245, 391
HpySE526I	ACGT	2 cut(s)	891, 1177
Hsp92I	GRCGYC	2 cut(s)	891, 1177
Hsp92II	CATG	6 cut(s)	73, 280, 691, 874, 916, 1301
KpnI	GGTACC	1 cut(s)	956
Ksp22I	TGATCA	1 cut(s)	759
KspAI	GTTAAC	2 cut(s)	808, 1123
Kzo9I	GATC	3 cut(s)	87, 586, 759
LmnI	GCTCC	4 cut(s)	515, 705, 850, 1283
Lsp1109I	GCAGC	6 cut(s)	298, 388, 392, 692, 994, 1090
LweI	GCATC	1 cut(s)	1045
MaeI	CTAG	3 cut(s)	405, 608, 1113
MaeII	ACGT	2 cut(s)	891, 1177
MaeIII	GTNAC	3 cut(s)	179, 619, 664
MalI	GATC	3 cut(s)	89, 588, 761
MboI	GATC	3 cut(s)	87, 586, 759
MboII	GAAGA	4 cut(s)	148, 227, 799, 949
MfeI	CAATTG	2 cut(s)	18, 1287
MhlI	GDGCHC	1 cut(s)	702
MlsI	TGGCCA	2 cut(s)	5, 1254
MluCI	AATT	8 cut(s)	18, 198, 576, 732, 1044, 1162, 1182, 1287
MluNI	TGGCCA	2 cut(s)	5, 1254
MlyI	GAGTC	1 cut(s)	976
MmeI	TCCRAC	1 cut(s)	316
MnlI	CCTC	9 cut(s)	151, 251, 429, 688, 812, 829, 892, 966, 982
Mox20I	TGGCCA	2 cut(s)	5, 1254
Mph1103I	ATGCAT	1 cut(s)	1299
MscI	TGGCCA	2 cut(s)	5, 1254
MseI	TTAA	3 cut(s)	807, 1014, 1122
Msp20I	TGGCCA	2 cut(s)	5, 1254
MspA1I	CMGCKG	2 cut(s)	401, 1103
MspI	CCGG	1 cut(s)	1063
MspR9I	CCNGG	8 cut(s)	75, 348, 596, 921, 1022, 1064, 1272, 1307
MunI	CAATTG	2 cut(s)	18, 1287
Mva1269I	GAATGC	2 cut(s)	946, 1093
MvaI	CCWGG	7 cut(s)	75, 348, 596, 921, 1022, 1272, 1307
MwoI	GCNNNNNNNGC	4 cut(s)	377, 493, 1004, 1283
NciI	CCSGG	1 cut(s)	1064
NdeI	CATATG	1 cut(s)	538
NdeII	GATC	3 cut(s)	87, 586, 759
NlaIII	CATG	6 cut(s)	73, 280, 691, 874, 916, 1301
NlaIV	GGNNCC	1 cut(s)	954
NmuCI	GTSAC	2 cut(s)	179, 664
NsiI	ATGCAT	1 cut(s)	1299
PaeR7I	CTCGAG	1 cut(s)	984
PceI	AGGCCT	1 cut(s)	1133
PctI	GAATGC	2 cut(s)	946, 1093
PfoI	TCCNGGA	1 cut(s)	346
PkrI	GCNGC	6 cut(s)	313, 382, 403, 682, 1009, 1105
PleI	GAGTC	1 cut(s)	976
PpsI	GAGTC	1 cut(s)	976
Psp6I	CCWGG	7 cut(s)	73, 346, 594, 919, 1020, 1270, 1305
PspGI	CCWGG	7 cut(s)	73, 346, 594, 919, 1020, 1270, 1305
PspN4I	GGNNCC	1 cut(s)	954
PspPI	GGNCC	1 cut(s)	821
PsrI	GAACNNNNNNTAC	2 cut(s)	563, 595
PstI	CTGCAG	1 cut(s)	1214
PstNI	CAGNNNCTG	1 cut(s)	1312
PvuII	CAGCTG	2 cut(s)	401, 1103
RsaI	GTAC	5 cut(s)	29, 47, 354, 593, 954
RsaNI	GTAC	5 cut(s)	28, 46, 353, 592, 953
SaqAI	TTAA	3 cut(s)	807, 1014, 1122
SatI	GCNGC	6 cut(s)	312, 381, 402, 681, 1008, 1104
Sau3AI	GATC	3 cut(s)	87, 586, 759
Sau96I	GGNCC	1 cut(s)	821
ScaI	AGTACT	1 cut(s)	354
SchI	GAGTC	1 cut(s)	976
ScrFI	CCNGG	8 cut(s)	75, 348, 596, 921, 1022, 1064, 1272, 1307
SduI	GDGCHC	1 cut(s)	702
SfaNI	GCATC	1 cut(s)	1045
SfcI	CTRYAG	4 cut(s)	112, 366, 811, 1210
Sfr274I	CTCGAG	1 cut(s)	984
SlaI	CTCGAG	1 cut(s)	984
SmlI	CTYRAG	1 cut(s)	984
SmoI	CTYRAG	1 cut(s)	984
Sse9I	AATT	8 cut(s)	18, 198, 576, 732, 1044, 1162, 1182, 1287
SseBI	AGGCCT	1 cut(s)	1133
SspMI	CTAG	3 cut(s)	405, 608, 1113
StuI	AGGCCT	1 cut(s)	1133
StyD4I	CCNGG	8 cut(s)	73, 346, 594, 919, 1020, 1062, 1270, 1305
StyI	CCWWGG	2 cut(s)	1128, 1134
TaaI	ACNGT	4 cut(s)	113, 502, 812, 1323
TaiI	ACGT	2 cut(s)	894, 1180
TaqI	TCGA	5 cut(s)	738, 877, 985, 1180, 1316
TasI	AATT	8 cut(s)	18, 198, 576, 732, 1044, 1162, 1182, 1287
TatI	WGTACW	3 cut(s)	27, 45, 352
Tru1I	TTAA	3 cut(s)	807, 1014, 1122
Tru9I	TTAA	3 cut(s)	807, 1014, 1122
TscAI	CASTG	2 cut(s)	249, 1214
TseFI	GTSAC	2 cut(s)	179, 664
TseI	GCWGC	6 cut(s)	311, 380, 401, 680, 1007, 1103
Tsp45I	GTSAC	2 cut(s)	179, 664
TspDTI	ATGAA	3 cut(s)	584, 800, 929
TspRI	CASTG	2 cut(s)	249, 1214
XapI	RAATTY	1 cut(s)	1182
XcmI	CCANNNNNNNNNTGG	1 cut(s)	472
XhoI	CTCGAG	1 cut(s)	984
XspI	CTAG	3 cut(s)	405, 608, 1113
ZraI	GACGTC	2 cut(s)	892, 1178
ZrmI	AGTACT	1 cut(s)	354
Zsp2I	ATGCAT	1 cut(s)	1299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.