Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002969

Physical Location & Seq

Genomic Coordinates Reverse (-)

7081763 .. 7084865

Gene Structure 3103 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G16370.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1560 bp

ATGGCAACAACTACTCTCAATGACAGTGTCACCACTACACTTGCTTCAGAGCCTCAAAGGACTTACCAAGTTGTTGTTGCTGCAACTAAAGAAATGGGTATTGGTAAAGATGGGAAATTGCCATGGAATTTGCCAACAGATCTCAAGTTCTTTAAAGACATTACTTTGACCACTTCAGATTCCTCTAAGAAAAATGCTGTTGTGATGGGTAGAAAGACTTGGGAGTCTATTCCCATTAAGTATAGGCCGCTTTCGGGTCGGCTTAACGTTGTTCTAACTCGTTCTGGTGGGTTTGATATAGCCAACACTGAGAATGTTGTCACTTGTAGTAGTGTAGATTCTGCTCTTGATTTGTTAGCTGCGCCGCCGTATTGTTTATCTATTGAGAGGGTTTTTGTTATAGGAGGTGGTGACATATTGAGGGAAGCATTGAATAGGCCTAGTTGTGATGCTATCCATTTAACTGAGATTGATACAAGTGTTGACTGTGATACGTTTATACCTGCGATTGATACTTCTGTTTATCAGCCTTGGTCTTCATCGTTTCCAGTAACTGAAAATGGACTTCGGTTTTGCTTCACGACTTTTGTCCGTGTAAAGAGTTCTGCTGATGAATCTTCTGATGAAAGCAATGGGTCACAGTCTCTTCAATTTGATGGGAAGAAGTTTTTGTTTCTTCCTAAGATGGTTTTTGATCAGCATGAGGAGTTTCTGTATTTGAATATGGTTGAAGATATTATCTCTAATGGCAATGTGAAGAATGATAGGACCGGGACTGGTACATTATCAAAATTTGGTTGCCAGATGAAATTCAATTTACGCAGAAGTTTTCCACTTCTGACAACAAAGCGAGTTTTCTGGAGAGGTGTTGTTGAGGAACTTCTATGGTTCATAAGCGGTTCAACCAATGCAAAAGTCCTTCAGGAAAAAGGTATCCATATATGGGATGGGAATGCGTCAAGAGAGTATCTCGATGGTATCGGCCTGACAGAGAGAGAGGAAGGCGACCTTGGACCTGTATACGGATTTCAATGGCGACACTTTGGTGCTAAGTACACAGATATGCATGCTGATTATACTGGTCAAGGATTTGATCAACTTGTAGATGTAATTGACAAAATCAAGAACAATCCTGATGATCGGCGTATTATAATGTCAGCTTGGAATCCTTCTGATCTTAAGCTGATGGCACTTCCTCCATGCCATATGTTTGCACAATTTTATGTGGCAGAAGGGGAACTCTCATGTCAAATGTATCAGCGTTCAGCAGATATGGGCCTTGGTGTGCCGTTTAACATTGCTTCTTACTCTCTTCTTACTTGCATGCTGGCTCATGTGTGTGATCTTGTTCCTGGTGATTTTATCCATGTTCTTGGGGATGCTCATGTATACAAAACTCACGTGAGGCCTCTGCAAGAGCAACTTCTGAATCTTCCAAAACCCTTTCCTGTAATGAAGATAAACCCGGAGAAGAAACAAATCGATTCTTTTGTGGCTTCTGACTTTGACCTCACAGGCTATGATCCTCACAAGAAGATAGAAATGAAAATGGCGGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004146 GO:0004799 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0009058 GO:0009108 GO:0009256 GO:0009257 GO:0009396 GO:0009987 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016740 GO:0016741 GO:0018130 GO:0019438 GO:0019752 GO:0032259 GO:0034641 GO:0042083 GO:0042398 GO:0042558 GO:0042559 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko00670 ko00790 ko01100 map00240 map00670 map00790 map01100

Pfam Domains

Protein Families

DHFR_1 Thymidylat_synt

Protein Analysis

519

Amino Acids

58.14

Weight (kDa)

5.55

Isoelectric Point (pI)

45.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHFR_1	PF00186	23 - 198	2.5e-36	Dihydrofolate reductase
Thymidylat_synt	PF00303	239 - 408	6.1e-63	Thymidylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16370 AT2G16370 AT2G16370 AT4G34570 AT4G34570 AT4G34570 AT4G34570
fragaria_vesca	FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370
malus_domestica	MD05G1063200.v1.1 MD10G1070700.v1.1
prunus_persica	Prupe.8G092500_v2.0.a1 Prupe.8G092500_v2.0.a1
pyrus_communis	pycom05g05460 pycom05g05470 pycom10g05650
rosa_chinensis	RchiOBHm_Chr6g0265641
rosa_laevigata	RLG00000014170
rosa_multiflora	Rmu_sc0003233.1_g000014 Rmu_sc0003233.1_g000015 Rmu_sc0009907.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432460 Rroxscaffold_6G00388530 Rroxscaffold_7G00202550
rosa_rugosa	Rorug06G0018100
rosa_samantha	Rh1BG086900 Rh3BG370300 Rh3CG367200 Rh6AG140100 Rh6BG140000 Rh6CG137100 Rh6DG123400
rosa_wichuraiana	Rw6G012050 Rw6G012100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1151
AasI	GACNNNNNNGTC	1 cut(s)	223
Acc36I	ACCTGC	1 cut(s)	511
AccI	GTMKAC	2 cut(s)	1020, 1389
AciI	CCGC	4 cut(s)	248, 365, 897, 1553
AclI	AACGTT	1 cut(s)	267
AclWI	GGATC	1 cut(s)	1517
AcsI	RAATTY	3 cut(s)	127, 791, 809
AcuI	CTGAAG	3 cut(s)	30, 159, 905
AcvI	CACGTG	1 cut(s)	1402
AfaI	GTAC	2 cut(s)	781, 1055
AfiI	CCNNNNNNNGG	3 cut(s)	254, 943, 1022
AflII	CTTAAG	1 cut(s)	1178
AgsI	TTSAA	7 cut(s)	433, 650, 721, 731, 814, 903, 1031
AjnI	CCWGG	1 cut(s)	1351
AjuI	GAANNNNNNNTTGG	4 cut(s)	84, 116, 993, 1025
AleI	CACNNNNGTG	1 cut(s)	1044
AluBI	AGCT	3 cut(s)	359, 1160, 1183
AluI	AGCT	3 cut(s)	359, 1160, 1183
Alw26I	GTCTC	1 cut(s)	648
AlwI	GGATC	1 cut(s)	1517
AlwNI	CAGNNNCTG	1 cut(s)	554
AoxI	GGCC	5 cut(s)	245, 437, 982, 1276, 1406
ApeKI	GCWGC	2 cut(s)	80, 359
ApoI	RAATTY	3 cut(s)	127, 791, 809
ArsI	GACNNNNNNTTYG	2 cut(s)	555, 587
AspLEI	GCGC	1 cut(s)	364
AspS9I	GGNCC	3 cut(s)	768, 1013, 1276
AsuC2I	CCSGG	2 cut(s)	772, 1466
AsuHPI	GGTGA	3 cut(s)	22, 422, 1367
AvaII	GGWCC	2 cut(s)	768, 1013
BaeI	ACNNNNGTAYC	4 cut(s)	465, 498, 504, 537
BbrPI	CACGTG	1 cut(s)	1402
BbsI	GAAGAC	1 cut(s)	528
BbvI	GCAGC	2 cut(s)	67, 346
BccI	CCATC	7 cut(s)	104, 199, 650, 679, 941, 968, 1180
BceAI	ACGGC	2 cut(s)	352, 1273
BciT130I	CCWGG	1 cut(s)	1353
BciVI	GTATCC	1 cut(s)	944
BclI	TGATCA	2 cut(s)	694, 1093
BcnI	CCSGG	2 cut(s)	772, 1466
BcoDI	GTCTC	1 cut(s)	648
BfaI	CTAG	1 cut(s)	441
BfrI	CTTAAG	1 cut(s)	1178
BfuAI	ACCTGC	1 cut(s)	511
BfuI	GTATCC	1 cut(s)	944
BglII	AGATCT	1 cut(s)	139
BisI	GCNGC	4 cut(s)	81, 248, 360, 365
BlsI	GCNGC	4 cut(s)	82, 249, 361, 366
Bme1390I	CCNGG	3 cut(s)	772, 1353, 1466
Bme18I	GGWCC	2 cut(s)	768, 1013
BmgT120I	GGNCC	3 cut(s)	768, 1013, 1276
BmrFI	CCNGG	3 cut(s)	772, 1353, 1466
BmsI	GCATC	2 cut(s)	439, 1369
BoxI	GACNNNNGTC	1 cut(s)	587
BpiI	GAAGAC	1 cut(s)	528
BplI	GAGNNNNNCTC	2 cut(s)	954, 986
BpmI	CTGGAG	1 cut(s)	880
BpuEI	CTTGAG	1 cut(s)	128
BpuMI	CCSGG	2 cut(s)	772, 1466
Bsa29I	ATCGAT	1 cut(s)	1482
BsaAI	YACGTR	1 cut(s)	1402
BsaJI	CCNNGG	4 cut(s)	122, 530, 1009, 1279
BsaXI	ACNNNNNCTCC	2 cut(s)	698, 728
Bsc4I	CCNNNNNNNGG	3 cut(s)	254, 943, 1022
Bse1I	ACTGG	3 cut(s)	548, 781, 1084
Bse3DI	GCAATG	3 cut(s)	637, 757, 1296
BseBI	CCWGG	1 cut(s)	1353
BseCI	ATCGAT	1 cut(s)	1482
BseDI	CCNNGG	4 cut(s)	122, 530, 1009, 1279
BseGI	GGATG	2 cut(s)	952, 1384
BseLI	CCNNNNNNNGG	3 cut(s)	254, 943, 1022
BseMI	GCAATG	3 cut(s)	637, 757, 1296
BseMII	CTCAG	2 cut(s)	300, 456
BseNI	ACTGG	3 cut(s)	548, 781, 1084
BseRI	GAGGAG	1 cut(s)	719
BseXI	GCAGC	2 cut(s)	67, 346
BshFI	GGCC	5 cut(s)	247, 439, 984, 1278, 1408
BshVI	ATCGAT	1 cut(s)	1482
BsiSI	CCGG	2 cut(s)	771, 1466
BslFI	GGGAC	1 cut(s)	787
BslI	CCNNNNNNNGG	3 cut(s)	254, 943, 1022
BsmAI	GTCTC	1 cut(s)	648
BsmFI	GGGAC	1 cut(s)	787
BsmI	GAATGC	1 cut(s)	958
BsnI	GGCC	5 cut(s)	247, 439, 984, 1278, 1408
Bsp143I	GATC	7 cut(s)	139, 694, 1093, 1138, 1174, 1342, 1522
Bsp19I	CCATGG	1 cut(s)	122
BspACI	CCGC	4 cut(s)	248, 365, 897, 1553
BspANI	GGCC	5 cut(s)	247, 439, 984, 1278, 1408
BspCNI	CTCAG	2 cut(s)	301, 457
BspDI	ATCGAT	1 cut(s)	1482
BspMI	ACCTGC	1 cut(s)	511
BspPI	GGATC	1 cut(s)	1517
BspTI	CTTAAG	1 cut(s)	1178
BsrDI	GCAATG	3 cut(s)	637, 757, 1296
BsrI	ACTGG	3 cut(s)	548, 781, 1084
BssECI	CCNNGG	4 cut(s)	122, 530, 1009, 1279
BssMI	GATC	7 cut(s)	139, 694, 1093, 1138, 1174, 1342, 1522
BssNAI	GTATAC	2 cut(s)	1021, 1390
BssT1I	CCWWGG	4 cut(s)	122, 530, 1009, 1279
Bst1107I	GTATAC	2 cut(s)	1021, 1390
Bst2UI	CCWGG	1 cut(s)	1353
Bst4CI	ACNGT	3 cut(s)	26, 488, 642
Bst6I	CTCTTC	2 cut(s)	651, 1317
BstAFI	CTTAAG	1 cut(s)	1178
BstBAI	YACGTR	1 cut(s)	1402
BstC8I	GCNNGC	3 cut(s)	1068, 1325, 1329
BstDEI	CTNAG	5 cut(s)	186, 309, 465, 681, 1050
BstDSI	CCRYGG	1 cut(s)	122
BstF5I	GGATG	2 cut(s)	952, 1384
BstHHI	GCGC	1 cut(s)	364
BstKTI	GATC	7 cut(s)	142, 697, 1096, 1141, 1177, 1345, 1525
BstMAI	GTCTC	1 cut(s)	648
BstMBI	GATC	7 cut(s)	139, 694, 1093, 1138, 1174, 1342, 1522
BstNI	CCWGG	1 cut(s)	1353
BstNSI	RCATGY	2 cut(s)	1070, 1327
BstPAI	GACNNNNGTC	1 cut(s)	587
BstSCI	CCNGG	3 cut(s)	770, 1351, 1464
BstV1I	GCAGC	2 cut(s)	67, 346
BstV2I	GAAGAC	1 cut(s)	528
BstX2I	RGATCY	1 cut(s)	139
BstXI	CCANNNNNNTGG	1 cut(s)	1373
BstYI	RGATCY	1 cut(s)	139
BstZ17I	GTATAC	2 cut(s)	1021, 1390
Bsu15I	ATCGAT	1 cut(s)	1482
BsuI	GTATCC	1 cut(s)	944
BsuRI	GGCC	5 cut(s)	247, 439, 984, 1278, 1408
BsuTUI	ATCGAT	1 cut(s)	1482
BtgI	CCRYGG	1 cut(s)	122
BtsCI	GGATG	2 cut(s)	952, 1384
BtsIMutI	CAGTG	2 cut(s)	31, 306
BveI	ACCTGC	1 cut(s)	511
Cac8I	GCNNGC	3 cut(s)	1068, 1325, 1329
CaiI	CAGNNNCTG	1 cut(s)	554
CfoI	GCGC	1 cut(s)	364
Cfr13I	GGNCC	3 cut(s)	768, 1013, 1276
ClaI	ATCGAT	1 cut(s)	1482
CseI	GACGC	1 cut(s)	945
Csp6I	GTAC	2 cut(s)	780, 1054
CspCI	CAANNNNNGTGG	2 cut(s)	22, 57
CviAII	CATG	9 cut(s)	123, 701, 1067, 1200, 1245, 1324, 1334, 1367, 1385
CviQI	GTAC	2 cut(s)	780, 1054
DdeI	CTNAG	5 cut(s)	186, 309, 465, 681, 1050
DpnI	GATC	7 cut(s)	141, 696, 1095, 1140, 1176, 1344, 1524
DpnII	GATC	7 cut(s)	139, 694, 1093, 1138, 1174, 1342, 1522
DraI	TTTAAA	1 cut(s)	154
DrdI	GACNNNNNNGTC	1 cut(s)	223
DseDI	GACNNNNNNGTC	1 cut(s)	223
Eam1104I	CTCTTC	2 cut(s)	651, 1317
EarI	CTCTTC	2 cut(s)	651, 1317
Eco130I	CCWWGG	4 cut(s)	122, 530, 1009, 1279
Eco147I	AGGCCT	2 cut(s)	439, 1408
Eco47I	GGWCC	2 cut(s)	768, 1013
Eco57I	CTGAAG	3 cut(s)	30, 159, 905
Eco72I	CACGTG	1 cut(s)	1402
EcoRII	CCWGG	1 cut(s)	1351
EcoT14I	CCWWGG	4 cut(s)	122, 530, 1009, 1279
EcoT22I	ATGCAT	1 cut(s)	1068
ErhI	CCWWGG	4 cut(s)	122, 530, 1009, 1279
FaeI	CATG	9 cut(s)	126, 704, 1070, 1203, 1248, 1327, 1337, 1370, 1388
FalI	AAGNNNNNCTT	4 cut(s)	993, 1025, 1407, 1439
FaqI	GGGAC	1 cut(s)	787
FatI	CATG	9 cut(s)	122, 700, 1066, 1199, 1244, 1323, 1333, 1366, 1384
FauNDI	CATATG	1 cut(s)	1206
FbaI	TGATCA	2 cut(s)	694, 1093
FblI	GTMKAC	2 cut(s)	1020, 1389
Fnu4HI	GCNGC	4 cut(s)	81, 248, 360, 365
FokI	GGATG	2 cut(s)	959, 1391
Fsp4HI	GCNGC	4 cut(s)	81, 248, 360, 365
FspBI	CTAG	1 cut(s)	441
GlaI	GCGC	1 cut(s)	363
GluI	GCNGC	4 cut(s)	81, 248, 360, 365
GsuI	CTGGAG	1 cut(s)	880
HaeIII	GGCC	5 cut(s)	247, 439, 984, 1278, 1408
HapII	CCGG	2 cut(s)	771, 1466
HgaI	GACGC	1 cut(s)	945
HhaI	GCGC	1 cut(s)	364
Hin1II	CATG	9 cut(s)	126, 704, 1070, 1203, 1248, 1327, 1337, 1370, 1388
Hin6I	GCGC	1 cut(s)	362
HinP1I	GCGC	1 cut(s)	362
HincII	GTYRAC	1 cut(s)	484
HindII	GTYRAC	1 cut(s)	484
HinfI	GANTC	7 cut(s)	179, 224, 338, 614, 1165, 1429, 1484
HpaII	CCGG	2 cut(s)	771, 1466
HphI	GGTGA	3 cut(s)	22, 422, 1367
Hpy166II	GTNNAC	4 cut(s)	484, 1021, 1056, 1390
Hpy188I	TCNGA	7 cut(s)	49, 178, 622, 840, 1174, 1428, 1501
Hpy188III	TCNNGA	8 cut(s)	347, 580, 859, 923, 960, 971, 1123, 1133
Hpy8I	GTNNAC	4 cut(s)	484, 1021, 1056, 1390
HpyAV	CCTTC	4 cut(s)	929, 995, 1179, 1226
HpyCH4III	ACNGT	3 cut(s)	26, 488, 642
HpyCH4IV	ACGT	3 cut(s)	267, 494, 1401
HpyCH4V	TGCA	6 cut(s)	83, 911, 1066, 1214, 1323, 1414
HpyF3I	CTNAG	5 cut(s)	186, 309, 465, 681, 1050
HpySE526I	ACGT	3 cut(s)	267, 494, 1401
Hsp92II	CATG	9 cut(s)	126, 704, 1070, 1203, 1248, 1327, 1337, 1370, 1388
HspAI	GCGC	1 cut(s)	362
Ksp22I	TGATCA	2 cut(s)	694, 1093
Kzo9I	GATC	7 cut(s)	139, 694, 1093, 1138, 1174, 1342, 1522
Lsp1109I	GCAGC	2 cut(s)	67, 346
LweI	GCATC	2 cut(s)	439, 1369
MaeI	CTAG	1 cut(s)	441
MaeII	ACGT	3 cut(s)	267, 494, 1401
MaeIII	GTNAC	5 cut(s)	28, 319, 410, 550, 636
MalI	GATC	7 cut(s)	141, 696, 1095, 1140, 1176, 1344, 1524
MboI	GATC	7 cut(s)	139, 694, 1093, 1138, 1174, 1342, 1522
MflI	RGATCY	1 cut(s)	139
MluCI	AATT	8 cut(s)	116, 127, 650, 791, 809, 814, 1110, 1217
MlyI	GAGTC	1 cut(s)	233
Mph1103I	ATGCAT	1 cut(s)	1068
MseI	TTAA	6 cut(s)	153, 237, 264, 461, 1179, 1293
MslI	CAYNNNNRTG	3 cut(s)	1044, 1061, 1338
MspCI	CTTAAG	1 cut(s)	1178
MspI	CCGG	2 cut(s)	771, 1466
MspR9I	CCNGG	3 cut(s)	772, 1353, 1466
Mva1269I	GAATGC	1 cut(s)	958
MvaI	CCWGG	1 cut(s)	1353
NciI	CCSGG	2 cut(s)	772, 1466
NcoI	CCATGG	1 cut(s)	122
NdeI	CATATG	1 cut(s)	1206
NdeII	GATC	7 cut(s)	139, 694, 1093, 1138, 1174, 1342, 1522
NlaIII	CATG	9 cut(s)	126, 704, 1070, 1203, 1248, 1327, 1337, 1370, 1388
NmuCI	GTSAC	4 cut(s)	28, 319, 410, 636
NsiI	ATGCAT	1 cut(s)	1068
NspI	RCATGY	2 cut(s)	1070, 1327
OliI	CACNNNNGTG	1 cut(s)	1044
PaeI	GCATGC	2 cut(s)	1070, 1327
PceI	AGGCCT	2 cut(s)	439, 1408
PctI	GAATGC	1 cut(s)	958
PfeI	GAWTC	6 cut(s)	179, 338, 614, 1165, 1429, 1484
PflFI	GACNNNGTC	1 cut(s)	26
PkrI	GCNGC	4 cut(s)	82, 249, 361, 366
PleI	GAGTC	1 cut(s)	232
PmaCI	CACGTG	1 cut(s)	1402
PmlI	CACGTG	1 cut(s)	1402
PpsI	GAGTC	1 cut(s)	232
Ppu21I	YACGTR	1 cut(s)	1402
PshAI	GACNNNNGTC	1 cut(s)	587
PsiI	TTATAA	1 cut(s)	1151
Psp1406I	AACGTT	1 cut(s)	267
Psp6I	CCWGG	1 cut(s)	1351
PspCI	CACGTG	1 cut(s)	1402
PspGI	CCWGG	1 cut(s)	1351
PspPI	GGNCC	3 cut(s)	768, 1013, 1276
PstNI	CAGNNNCTG	1 cut(s)	554
PsuI	RGATCY	1 cut(s)	139
PsyI	GACNNNGTC	1 cut(s)	26
RsaI	GTAC	2 cut(s)	781, 1055
RsaNI	GTAC	2 cut(s)	780, 1054
RseI	CAYNNNNRTG	3 cut(s)	1044, 1061, 1338
SaqAI	TTAA	6 cut(s)	153, 237, 264, 461, 1179, 1293
SatI	GCNGC	4 cut(s)	81, 248, 360, 365
Sau3AI	GATC	7 cut(s)	139, 694, 1093, 1138, 1174, 1342, 1522
Sau96I	GGNCC	3 cut(s)	768, 1013, 1276
SchI	GAGTC	1 cut(s)	233
ScrFI	CCNGG	3 cut(s)	772, 1353, 1466
SfaNI	GCATC	2 cut(s)	439, 1369
SinI	GGWCC	2 cut(s)	768, 1013
SmiMI	CAYNNNNRTG	3 cut(s)	1044, 1061, 1338
SmlI	CTYRAG	2 cut(s)	143, 1178
SmoI	CTYRAG	2 cut(s)	143, 1178
SphI	GCATGC	2 cut(s)	1070, 1327
Sse9I	AATT	8 cut(s)	116, 127, 650, 791, 809, 814, 1110, 1217
SseBI	AGGCCT	2 cut(s)	439, 1408
SsiI	CCGC	4 cut(s)	248, 365, 897, 1553
SspMI	CTAG	1 cut(s)	441
StuI	AGGCCT	2 cut(s)	439, 1408
StyD4I	CCNGG	3 cut(s)	770, 1351, 1464
StyI	CCWWGG	4 cut(s)	122, 530, 1009, 1279
TaaI	ACNGT	3 cut(s)	26, 488, 642
TaiI	ACGT	3 cut(s)	270, 497, 1404
TaqI	TCGA	2 cut(s)	972, 1482
TasI	AATT	8 cut(s)	116, 127, 650, 791, 809, 814, 1110, 1217
TatI	WGTACW	1 cut(s)	1053
TauI	GCSGC	2 cut(s)	250, 367
TfiI	GAWTC	6 cut(s)	179, 338, 614, 1165, 1429, 1484
Tru1I	TTAA	6 cut(s)	153, 237, 264, 461, 1179, 1293
Tru9I	TTAA	6 cut(s)	153, 237, 264, 461, 1179, 1293
TscAI	CASTG	2 cut(s)	31, 313
TseFI	GTSAC	4 cut(s)	28, 319, 410, 636
TseI	GCWGC	2 cut(s)	80, 359
Tsp45I	GTSAC	4 cut(s)	28, 319, 410, 636
TspDTI	ATGAA	7 cut(s)	528, 627, 639, 821, 880, 1469, 1559
TspGWI	ACGGA	2 cut(s)	581, 1038
TspRI	CASTG	2 cut(s)	31, 313
Tth111I	GACNNNGTC	1 cut(s)	26
Vha464I	CTTAAG	1 cut(s)	1178
VpaK11BI	GGWCC	2 cut(s)	768, 1013
XapI	RAATTY	3 cut(s)	127, 791, 809
XceI	RCATGY	2 cut(s)	1070, 1327
XcmI	CCANNNNNNNNNTGG	1 cut(s)	944
XmiI	GTMKAC	2 cut(s)	1020, 1389
XspI	CTAG	1 cut(s)	441
Zsp2I	ATGCAT	1 cut(s)	1068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.