Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0002969

Physical Location & Seq

Genomic Coordinates Forward (+)

48588446 .. 48592595

Gene Structure 4150 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014170

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGCATACCCCGCCACAATGGACTTTGGCGGAACTCCGCGTCAACCGCTTCTTTGGATTTCTTCGCCTACATGTCATGTCACCCCAACTTGCTGCCACTCTTGCCTCCCTTCCCTCCAAAATTTCGCTCCACCGTTTAAGATCGGCCTTACCGCACTTCCAGAATAATTGGCTGTGCGGTCCTCGTTTGAAGTTGCTACCTTTGAGTCTGTGTCGTTCCTTGTATTCAACAATGGCTGGTGAAGTAGTGACCGTAAATGGAAATGGCAATGGCAATGGCAACACCCAGCTGAATCCACAGAGGACTTACCAAGTTGTTGTGGCTGCAACTCGAGAAATGGGTATTGGAAAGGATGGGAAACTACCTTGGAAACTTCCTTCTGATCTCAAGTTCTTTAAGGATGTTACTGTAACCACATCAGATCCCGGAAAAAAGAATGCAATTATAATGGGCAGAAAAACATGGGAGAGCATTCCCCCTGAGAACCGGCCTCTACCTGGACGCCTTAATGTTGTTCTGACTCGTTCTGGGAGTTTTGATATAGCTACTGCAGAGAATGTCGTCATATGTGGGAGCATGGCCTCTGCTCTGGAGCTGTTAGCTGCTTCCCCTTACTGTCTGTCAATTGACAAAGTGTTTCTCATAGGAGGTGGCCAGATATTGAGGGAAGCTCTCAATGGGCCTGGCTGTGATGCAATTCACATTACAGAAATAGAGACCAACATTGAATGCGATACTTTTATCCCTGCAATTGATTCCACTGTATTTCAGCCATGGTATTCATCTTTTCCCAAGGTGGAGAATAACATTCGTTGTTCCTTCACGACTTATGTGCGTGTGAGGAGTTCTGAAGTTGGATCCCTCTGTGAAAACAATGATCTGAACTGTAATAGTAGGTCGGATTCCATTAAGTGTGAAGTAAAGAAGTTCTCTTTCCTCCCGAAGATGATTTTCGAGAAACATGAGGAGTACATGTATCTAAATCTGGTTCAAGATATACTCTCAGATGGTACTTCTAAGGATGATAGGACTGGGACTGGTACTTTATCAAAATTTGGTTGCCAGATGCGGTTCAATCTGCGCAGAAGTTTTCCTCTTCTAACAACCAAGAAAGTATTTTGGCGAGGGGTTGTCGAAGAACTTCTGTGGTTCCTCAGTGGTTCAACCAATGCCAAGGTCCTTCAGGAGAAAGGCATCCGCATATGGGATGGAAATGCATCTAGAGACTACCTTGATAGCATTGGTTTAACTGGCAGGGAGGAGGGCGACTTAGGACCAGTATATGGGTTCCAGTGGCGACACTTTGGTGCCAGGTATACTGACATGCATGCTGACTACACTGGCCAAGGATTTGACCAGTTGTTAGATGTTATTGACAAGATCAAAAATAATCCTAATGATCGACGGATTATTCTTTCAGCTTGGAATCCTTCTGATCTGAAATTGATGGCACTTCCACCTTGTCACATGTTTGCTCAGTTCTATGTTGCAAAAGGAGAGTTATCATGCCAAATGTATCAGCGTTCTGCTGACATGGGCCTGGGCGTGCCATTTAACATCGCGTCATATGCGCTTCTGACATGCATGATTGCTCATGTCTGTGATCTTGTTCCAGGTGATTTCGTTCACGTTTTAGGGGATGCTCATATTTACCGTACTCACATCAGGCCTCTGCAGGAGCAACTTCAGAAACTGCCTAAGCCTCTTCCAATTTTGAAGATCAATCCTGATAAAAAGAATATAGATTCATTTGTGGCAGCTGATTTCGAACTCATAGGTTATAATCCTCATGAGAAGATAGAAATGAAAATGGCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004146 GO:0004799 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0009058 GO:0009108 GO:0009256 GO:0009257 GO:0009396 GO:0009987 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016740 GO:0016741 GO:0018130 GO:0019438 GO:0019752 GO:0032259 GO:0034641 GO:0042083 GO:0042398 GO:0042558 GO:0042559 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko00670 ko00790 ko01100 map00240 map00670 map00790 map01100

Pfam Domains

Protein Families

DHFR_1 Thymidylat_synt

Protein Analysis

607

Amino Acids

67.98

Weight (kDa)

8.49

Isoelectric Point (pI)

44.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHFR_1	PF00186	104 - 279	3.4e-37	Dihydrofolate reductase
Thymidylat_synt	PF00303	325 - 606	1.1e-116	Thymidylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16370 AT2G16370 AT2G16370 AT4G34570 AT4G34570 AT4G34570 AT4G34570
fragaria_vesca	FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370
malus_domestica	MD05G1063200.v1.1 MD10G1070700.v1.1
prunus_persica	Prupe.8G092500_v2.0.a1 Prupe.8G092500_v2.0.a1
pyrus_communis	pycom05g05460 pycom05g05470 pycom10g05650
rosa_chinensis	RchiOBHm_Chr6g0265641
rosa_laevigata	RLG00000014170
rosa_multiflora	Rmu_sc0003233.1_g000014 Rmu_sc0003233.1_g000015 Rmu_sc0009907.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432460 Rroxscaffold_6G00388530 Rroxscaffold_7G00202550
rosa_rugosa	Rorug06G0018100
rosa_samantha	Rh1BG086900 Rh3BG370300 Rh3CG367200 Rh6AG140100 Rh6BG140000 Rh6CG137100 Rh6DG123400
rosa_wichuraiana	Rw6G012050 Rw6G012100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	446, 1782
Acc16I	TGCGCA	1 cut(s)	1082
AccB1I	GGYRCC	1 cut(s)	1307
AccB7I	CCANNNNNTGG	1 cut(s)	1283
AccI	GTMKAC	1 cut(s)	1316
AccII	CGCG	2 cut(s)	39, 1562
AciI	CCGC	8 cut(s)	11, 29, 37, 46, 152, 177, 1069, 1198
AclWI	GGATC	3 cut(s)	416, 852, 865
AcoI	YGGCCR	2 cut(s)	652, 1342
AcsI	RAATTY	2 cut(s)	120, 1052
AcuI	CTGAAG	3 cut(s)	870, 1166, 1670
AcyI	GRCGYC	1 cut(s)	502
AfaI	GTAC	4 cut(s)	971, 1012, 1042, 1657
AfiI	CCNNNNNNNGG	3 cut(s)	497, 1204, 1283
AflIII	ACRYGT	3 cut(s)	70, 972, 1467
AgsI	TTSAA	7 cut(s)	190, 228, 728, 992, 1075, 1164, 1717
AjnI	CCWGG	5 cut(s)	496, 682, 1310, 1539, 1612
AjuI	GAANNNNNNNTTGG	2 cut(s)	327, 359
AleI	CACNNNNGTG	1 cut(s)	1305
AluBI	AGCT	7 cut(s)	289, 545, 595, 602, 671, 1421, 1760
AluI	AGCT	7 cut(s)	289, 545, 595, 602, 671, 1421, 1760
Alw26I	GTCTC	2 cut(s)	710, 1218
AlwI	GGATC	3 cut(s)	416, 852, 865
AlwNI	CAGNNNCTG	1 cut(s)	1693
Ama87I	CYCGRG	1 cut(s)	330
AoxI	GGCC	8 cut(s)	144, 488, 579, 652, 680, 1342, 1537, 1667
ApeKI	GCWGC	4 cut(s)	92, 323, 602, 1757
ApoI	RAATTY	2 cut(s)	120, 1052
Asp700I	GAANNNNTTC	1 cut(s)	1140
AspLEI	GCGC	2 cut(s)	1083, 1573
AspS9I	GGNCC	5 cut(s)	179, 680, 1177, 1274, 1537
AsuC2I	CCSGG	1 cut(s)	426
AsuHPI	GGTGA	3 cut(s)	72, 251, 1628
AsuII	TTCGAA	1 cut(s)	1767
AvaI	CYCGRG	1 cut(s)	330
AvaII	GGWCC	3 cut(s)	179, 1177, 1274
BalI	TGGCCA	2 cut(s)	654, 1344
BamHI	GGATCC	1 cut(s)	857
BanI	GGYRCC	1 cut(s)	1307
BbvI	GCAGC	4 cut(s)	79, 310, 589, 1769
BccI	CCATC	4 cut(s)	347, 1001, 1202, 1441
BcgI	CGANNNNNNTGC	2 cut(s)	814, 848
BciT130I	CCWGG	5 cut(s)	498, 684, 1312, 1541, 1614
BcnI	CCSGG	1 cut(s)	426
BcoDI	GTCTC	2 cut(s)	710, 1218
BfaI	CTAG	1 cut(s)	1221
BfmI	CTRYAG	2 cut(s)	549, 1673
BisI	GCNGC	4 cut(s)	93, 324, 603, 1758
BlsI	GCNGC	4 cut(s)	94, 325, 604, 1759
Bme1390I	CCNGG	6 cut(s)	426, 498, 684, 1312, 1541, 1614
Bme18I	GGWCC	3 cut(s)	179, 1177, 1274
BmeT110I	CYCGRG	1 cut(s)	330
BmgT120I	GGNCC	5 cut(s)	179, 680, 1177, 1274, 1537
BmiI	GGNNCC	4 cut(s)	859, 1151, 1289, 1309
BmrFI	CCNGG	6 cut(s)	426, 498, 684, 1312, 1541, 1614
BmrI	ACTGGG	1 cut(s)	1041
BmsI	GCATC	5 cut(s)	682, 1056, 1203, 1226, 1630
BmuI	ACTGGG	1 cut(s)	1041
BplI	GAGNNNNNCTC	2 cut(s)	655, 687
BpmI	CTGGAG	1 cut(s)	611
Bpu10I	CCTNAGC	1 cut(s)	1698
Bpu14I	TTCGAA	1 cut(s)	1767
BpuEI	CTTGAG	1 cut(s)	371
BpuMI	CCSGG	1 cut(s)	426
BsaHI	GRCGYC	1 cut(s)	502
BsaI	GGTCTC	1 cut(s)	710
BsaJI	CCNNGG	6 cut(s)	365, 773, 792, 1173, 1345, 1540
BsaXI	ACNNNNNCTCC	2 cut(s)	959, 989
Bsc4I	CCNNNNNNNGG	3 cut(s)	497, 1204, 1283
Bse118I	RCCGGY	1 cut(s)	486
Bse1I	ACTGG	7 cut(s)	1036, 1042, 1255, 1277, 1291, 1345, 1357
Bse3DI	GCAATG	2 cut(s)	274, 280
BseBI	CCWGG	5 cut(s)	498, 684, 1312, 1541, 1614
BseDI	CCNNGG	6 cut(s)	365, 773, 792, 1173, 1345, 1540
BseGI	GGATG	6 cut(s)	358, 406, 1027, 1194, 1213, 1645
BseLI	CCNNNNNNNGG	3 cut(s)	497, 1204, 1283
BseMI	GCAATG	2 cut(s)	274, 280
BseMII	CTCAG	4 cut(s)	471, 1017, 1168, 1490
BseNI	ACTGG	7 cut(s)	1036, 1042, 1255, 1277, 1291, 1345, 1357
BseRI	GAGGAG	3 cut(s)	856, 980, 1274
BseXI	GCAGC	4 cut(s)	79, 310, 589, 1769
BseYI	CCCAGC	1 cut(s)	285
Bsh1236I	CGCG	2 cut(s)	39, 1562
BshFI	GGCC	8 cut(s)	146, 490, 581, 654, 682, 1344, 1539, 1669
BshNI	GGYRCC	1 cut(s)	1307
BsiHKCI	CYCGRG	1 cut(s)	330
BsiSI	CCGG	2 cut(s)	426, 487
BslFI	GGGAC	1 cut(s)	1048
BslI	CCNNNNNNNGG	3 cut(s)	497, 1204, 1283
BsmAI	GTCTC	2 cut(s)	710, 1218
BsmFI	GGGAC	1 cut(s)	1048
BsmI	GAATGC	3 cut(s)	442, 471, 734
BsnI	GGCC	8 cut(s)	146, 490, 581, 654, 682, 1344, 1539, 1669
Bso31I	GGTCTC	1 cut(s)	710
BsoBI	CYCGRG	1 cut(s)	330
Bsp119I	TTCGAA	1 cut(s)	1767
Bsp19I	CCATGG	1 cut(s)	773
BspACI	CCGC	8 cut(s)	11, 29, 37, 46, 152, 177, 1069, 1198
BspANI	GGCC	8 cut(s)	146, 490, 581, 654, 682, 1344, 1539, 1669
BspCNI	CTCAG	4 cut(s)	472, 1016, 1167, 1489
BspFNI	CGCG	2 cut(s)	39, 1562
BspHI	TCATGA	1 cut(s)	1789
BspLI	GGNNCC	4 cut(s)	859, 1151, 1289, 1309
BspMAI	CTGCAG	2 cut(s)	553, 1677
BspPI	GGATC	3 cut(s)	416, 852, 865
BspT104I	TTCGAA	1 cut(s)	1767
BspT107I	GGYRCC	1 cut(s)	1307
BspTNI	GGTCTC	1 cut(s)	710
BsrDI	GCAATG	2 cut(s)	274, 280
BsrFI	RCCGGY	1 cut(s)	486
BsrI	ACTGG	7 cut(s)	1036, 1042, 1255, 1277, 1291, 1345, 1357
BssAI	RCCGGY	1 cut(s)	486
BssECI	CCNNGG	6 cut(s)	365, 773, 792, 1173, 1345, 1540
BssNAI	GTATAC	1 cut(s)	1317
BssNI	GRCGYC	1 cut(s)	502
BssT1I	CCWWGG	5 cut(s)	365, 773, 792, 1173, 1345
Bst1107I	GTATAC	1 cut(s)	1317
Bst2UI	CCWGG	5 cut(s)	498, 684, 1312, 1541, 1614
Bst4CI	ACNGT	7 cut(s)	134, 253, 409, 617, 763, 887, 1655
Bst6I	CTCTTC	2 cut(s)	1101, 1710
BstACI	GRCGYC	1 cut(s)	502
BstBI	TTCGAA	1 cut(s)	1767
BstC8I	GCNNGC	2 cut(s)	1329, 1547
BstDEI	CTNAG	7 cut(s)	480, 1003, 1017, 1154, 1270, 1476, 1698
BstDSI	CCRYGG	1 cut(s)	773
BstF5I	GGATG	6 cut(s)	358, 406, 1027, 1194, 1213, 1645
BstFNI	CGCG	2 cut(s)	39, 1562
BstHHI	GCGC	2 cut(s)	1083, 1573
BstMAI	GTCTC	2 cut(s)	710, 1218
BstMWI	GCNNNNNNNGC	4 cut(s)	10, 45, 101, 1568
BstNI	CCWGG	5 cut(s)	498, 684, 1312, 1541, 1614
BstNSI	RCATGY	6 cut(s)	74, 976, 1327, 1331, 1471, 1584
BstSCI	CCNGG	6 cut(s)	424, 496, 682, 1310, 1539, 1612
BstSFI	CTRYAG	2 cut(s)	549, 1673
BstUI	CGCG	2 cut(s)	39, 1562
BstV1I	GCAGC	4 cut(s)	79, 310, 589, 1769
BstX2I	RGATCY	2 cut(s)	421, 857
BstYI	RGATCY	2 cut(s)	421, 857
BstZ17I	GTATAC	1 cut(s)	1317
BsuRI	GGCC	8 cut(s)	146, 490, 581, 654, 682, 1344, 1539, 1669
BtgI	CCRYGG	1 cut(s)	773
BtgZI	GCGATG	1 cut(s)	1543
BtsCI	GGATG	6 cut(s)	358, 406, 1027, 1194, 1213, 1645
BtsIMutI	CAGTG	4 cut(s)	759, 1162, 1298, 1338
Cac8I	GCNNGC	2 cut(s)	1329, 1547
CaiI	CAGNNNCTG	1 cut(s)	1693
CciI	TCATGA	1 cut(s)	1789
CfoI	GCGC	2 cut(s)	1083, 1573
Cfr10I	RCCGGY	1 cut(s)	486
Cfr13I	GGNCC	5 cut(s)	179, 680, 1177, 1274, 1537
CseI	GACGC	3 cut(s)	28, 510, 1551
Csp6I	GTAC	4 cut(s)	970, 1011, 1041, 1656
CviQI	GTAC	4 cut(s)	970, 1011, 1041, 1656
DdeI	CTNAG	7 cut(s)	480, 1003, 1017, 1154, 1270, 1476, 1698
EaeI	YGGCCR	2 cut(s)	652, 1342
Eam1104I	CTCTTC	2 cut(s)	1101, 1710
EarI	CTCTTC	2 cut(s)	1101, 1710
EciI	GGCGGA	1 cut(s)	44
Eco130I	CCWWGG	5 cut(s)	365, 773, 792, 1173, 1345
Eco147I	AGGCCT	1 cut(s)	1669
Eco31I	GGTCTC	1 cut(s)	710
Eco47I	GGWCC	3 cut(s)	179, 1177, 1274
Eco57I	CTGAAG	3 cut(s)	870, 1166, 1670
Eco88I	CYCGRG	1 cut(s)	330
EcoO109I	RGGNCCY	1 cut(s)	1177
EcoRII	CCWGG	5 cut(s)	496, 682, 1310, 1539, 1612
EcoT14I	CCWWGG	5 cut(s)	365, 773, 792, 1173, 1345
EcoT22I	ATGCAT	4 cut(s)	6, 1219, 1329, 1586
ErhI	CCWWGG	5 cut(s)	365, 773, 792, 1173, 1345
FaqI	GGGAC	1 cut(s)	1048
FauI	CCCGC	1 cut(s)	18
FauNDI	CATATG	3 cut(s)	566, 1202, 1567
FblI	GTMKAC	1 cut(s)	1316
Fnu4HI	GCNGC	4 cut(s)	93, 324, 603, 1758
FokI	GGATG	6 cut(s)	365, 413, 1034, 1181, 1220, 1652
Fsp4HI	GCNGC	4 cut(s)	93, 324, 603, 1758
FspBI	CTAG	1 cut(s)	1221
FspI	TGCGCA	1 cut(s)	1082
GlaI	GCGC	2 cut(s)	1082, 1572
GluI	GCNGC	4 cut(s)	93, 324, 603, 1758
GsaI	CCCAGC	1 cut(s)	289
GsuI	CTGGAG	1 cut(s)	611
HaeIII	GGCC	8 cut(s)	146, 490, 581, 654, 682, 1344, 1539, 1669
HapII	CCGG	2 cut(s)	426, 487
HgaI	GACGC	3 cut(s)	28, 510, 1551
HhaI	GCGC	2 cut(s)	1083, 1573
Hin1I	GRCGYC	1 cut(s)	502
Hin6I	GCGC	2 cut(s)	1081, 1571
HinP1I	GCGC	2 cut(s)	1081, 1571
HincII	GTYRAC	1 cut(s)	43
HindII	GTYRAC	1 cut(s)	43
HinfI	GANTC	7 cut(s)	205, 292, 520, 755, 902, 1426, 1745
HpaII	CCGG	2 cut(s)	426, 487
HphI	GGTGA	3 cut(s)	72, 251, 1628
Hpy166II	GTNNAC	3 cut(s)	43, 1317, 1627
Hpy8I	GTNNAC	3 cut(s)	43, 1317, 1627
Hpy99I	CGWCG	1 cut(s)	1407
HpyAV	CCTTC	5 cut(s)	119, 387, 829, 1190, 1440
HpyCH4III	ACNGT	7 cut(s)	134, 253, 409, 617, 763, 887, 1655
HpyCH4IV	ACGT	1 cut(s)	1629
HpyF10VI	GCNNNNNNNGC	4 cut(s)	10, 45, 101, 1568
HpyF3I	CTNAG	7 cut(s)	480, 1003, 1017, 1154, 1270, 1476, 1698
HpySE526I	ACGT	1 cut(s)	1629
Hsp92I	GRCGYC	1 cut(s)	502
HspAI	GCGC	2 cut(s)	1081, 1571
LmnI	GCTCC	4 cut(s)	132, 573, 592, 1678
Lsp1109I	GCAGC	4 cut(s)	79, 310, 589, 1769
LweI	GCATC	5 cut(s)	682, 1056, 1203, 1226, 1630
MaeI	CTAG	1 cut(s)	1221
MaeII	ACGT	1 cut(s)	1629
MaeIII	GTNAC	5 cut(s)	78, 247, 403, 409, 1463
MboII	GAAGA	7 cut(s)	53, 955, 1088, 1148, 1697, 1729, 1807
MfeI	CAATTG	2 cut(s)	624, 750
MflI	RGATCY	2 cut(s)	421, 857
MlsI	TGGCCA	2 cut(s)	654, 1344
MluCI	AATT	9 cut(s)	120, 166, 441, 624, 696, 750, 1052, 1442, 1710
MluNI	TGGCCA	2 cut(s)	654, 1344
MlyI	GAGTC	2 cut(s)	214, 514
MmeI	TCCRAC	2 cut(s)	835, 879
Mox20I	TGGCCA	2 cut(s)	654, 1344
Mph1103I	ATGCAT	4 cut(s)	6, 1219, 1329, 1586
MroXI	GAANNNNTTC	1 cut(s)	1140
MscI	TGGCCA	2 cut(s)	654, 1344
MseI	TTAA	6 cut(s)	137, 396, 507, 909, 1247, 1554
MslI	CAYNNNNRTG	2 cut(s)	1305, 1599
Msp20I	TGGCCA	2 cut(s)	654, 1344
MspA1I	CMGCKG	2 cut(s)	289, 1760
MspI	CCGG	2 cut(s)	426, 487
MspR9I	CCNGG	6 cut(s)	426, 498, 684, 1312, 1541, 1614
MunI	CAATTG	2 cut(s)	624, 750
Mva1269I	GAATGC	3 cut(s)	442, 471, 734
MvaI	CCWGG	5 cut(s)	498, 684, 1312, 1541, 1614
MvnI	CGCG	2 cut(s)	39, 1562
MwoI	GCNNNNNNNGC	4 cut(s)	10, 45, 101, 1568
NciI	CCSGG	1 cut(s)	426
NcoI	CCATGG	1 cut(s)	773
NdeI	CATATG	3 cut(s)	566, 1202, 1567
NlaIV	GGNNCC	4 cut(s)	859, 1151, 1289, 1309
NmuCI	GTSAC	3 cut(s)	78, 247, 1463
NsbI	TGCGCA	1 cut(s)	1082
NsiI	ATGCAT	4 cut(s)	6, 1219, 1329, 1586
NspI	RCATGY	6 cut(s)	74, 976, 1327, 1331, 1471, 1584
NspV	TTCGAA	1 cut(s)	1767
OliI	CACNNNNGTG	1 cut(s)	1305
PaeI	GCATGC	1 cut(s)	1331
PaeR7I	CTCGAG	1 cut(s)	330
PagI	TCATGA	1 cut(s)	1789
PceI	AGGCCT	1 cut(s)	1669
PciI	ACATGT	3 cut(s)	70, 972, 1467
PctI	GAATGC	3 cut(s)	442, 471, 734
PdmI	GAANNNNTTC	1 cut(s)	1140
PfeI	GAWTC	5 cut(s)	292, 755, 902, 1426, 1745
PflMI	CCANNNNNTGG	1 cut(s)	1283
PfoI	TCCNGGA	1 cut(s)	424
PkrI	GCNGC	4 cut(s)	94, 325, 604, 1759
PleI	GAGTC	2 cut(s)	213, 514
PpsI	GAGTC	2 cut(s)	213, 514
PpuMI	RGGWCCY	1 cut(s)	1177
PscI	ACATGT	3 cut(s)	70, 972, 1467
PsiI	TTATAA	2 cut(s)	446, 1782
Psp5II	RGGWCCY	1 cut(s)	1177
Psp6I	CCWGG	5 cut(s)	496, 682, 1310, 1539, 1612
PspFI	CCCAGC	1 cut(s)	285
PspGI	CCWGG	5 cut(s)	496, 682, 1310, 1539, 1612
PspN4I	GGNNCC	4 cut(s)	859, 1151, 1289, 1309
PspPI	GGNCC	5 cut(s)	179, 680, 1177, 1274, 1537
PspPPI	RGGWCCY	1 cut(s)	1177
PstI	CTGCAG	2 cut(s)	553, 1677
PstNI	CAGNNNCTG	1 cut(s)	1693
PsuI	RGATCY	2 cut(s)	421, 857
PvuII	CAGCTG	2 cut(s)	289, 1760
RsaI	GTAC	4 cut(s)	971, 1012, 1042, 1657
RsaNI	GTAC	4 cut(s)	970, 1011, 1041, 1656
RseI	CAYNNNNRTG	2 cut(s)	1305, 1599
SaqAI	TTAA	6 cut(s)	137, 396, 507, 909, 1247, 1554
SatI	GCNGC	4 cut(s)	93, 324, 603, 1758
Sau96I	GGNCC	5 cut(s)	179, 680, 1177, 1274, 1537
SchI	GAGTC	2 cut(s)	214, 514
ScrFI	CCNGG	6 cut(s)	426, 498, 684, 1312, 1541, 1614
SfaNI	GCATC	5 cut(s)	682, 1056, 1203, 1226, 1630
SfcI	CTRYAG	2 cut(s)	549, 1673
Sfr274I	CTCGAG	1 cut(s)	330
SfuI	TTCGAA	1 cut(s)	1767
SinI	GGWCC	3 cut(s)	179, 1177, 1274
SlaI	CTCGAG	1 cut(s)	330
SmiMI	CAYNNNNRTG	2 cut(s)	1305, 1599
SmlI	CTYRAG	2 cut(s)	330, 386
SmoI	CTYRAG	2 cut(s)	330, 386
SphI	GCATGC	1 cut(s)	1331
Sse9I	AATT	9 cut(s)	120, 166, 441, 624, 696, 750, 1052, 1442, 1710
SseBI	AGGCCT	1 cut(s)	1669
SsiI	CCGC	8 cut(s)	11, 29, 37, 46, 152, 177, 1069, 1198
SspMI	CTAG	1 cut(s)	1221
StuI	AGGCCT	1 cut(s)	1669
StyD4I	CCNGG	6 cut(s)	424, 496, 682, 1310, 1539, 1612
StyI	CCWWGG	5 cut(s)	365, 773, 792, 1173, 1345
TaaI	ACNGT	7 cut(s)	134, 253, 409, 617, 763, 887, 1655
TaiI	ACGT	1 cut(s)	1632
TaqI	TCGA	5 cut(s)	331, 954, 1134, 1402, 1767
TasI	AATT	9 cut(s)	120, 166, 441, 624, 696, 750, 1052, 1442, 1710
TatI	WGTACW	1 cut(s)	969
TfiI	GAWTC	5 cut(s)	292, 755, 902, 1426, 1745
Tru1I	TTAA	6 cut(s)	137, 396, 507, 909, 1247, 1554
Tru9I	TTAA	6 cut(s)	137, 396, 507, 909, 1247, 1554
TscAI	CASTG	4 cut(s)	766, 1162, 1298, 1345
TseFI	GTSAC	3 cut(s)	78, 247, 1463
TseI	GCWGC	4 cut(s)	92, 323, 602, 1757
Tsp45I	GTSAC	3 cut(s)	78, 247, 1463
TspDTI	ATGAA	3 cut(s)	771, 1737, 1820
TspGWI	ACGGA	1 cut(s)	1420
TspRI	CASTG	4 cut(s)	766, 1162, 1298, 1345
Van91I	CCANNNNNTGG	1 cut(s)	1283
VpaK11BI	GGWCC	3 cut(s)	179, 1177, 1274
XapI	RAATTY	2 cut(s)	120, 1052
XbaI	TCTAGA	1 cut(s)	1220
XceI	RCATGY	6 cut(s)	74, 976, 1327, 1331, 1471, 1584
XhoI	CTCGAG	1 cut(s)	330
XmiI	GTMKAC	1 cut(s)	1316
XmnI	GAANNNNTTC	1 cut(s)	1140
XspI	CTAG	1 cut(s)	1221
Zsp2I	ATGCAT	4 cut(s)	6, 1219, 1329, 1586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.