Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000175

Orthogroup ID

OG0002969

Physical Location & Seq

Genomic Coordinates Reverse (-)

1209940 .. 1214661

Gene Structure 4722 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_175G00432460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGCTGGTGAAGTAGTGACCGTAAATGGAAATGGCAATGGCAATGGAAACACCCAGCTGAATCCACAGAGGACTTACCAAGTTGTTGTGGCTGCAACTCGAGAAATGGGTATTGGAAAGGATGGGAAACTACCTTGGAAACTTCCTTCTGATCTCAAGTTCTTTAAGGATGTCACTGTAACCACATCAGATCCTGGAAAAAAGAATGCAATTATAATGGGCAGAAAAACATGGGAGAGCATTCCCCCTGAGCATCGGCCTCTACCTGGACGCCTTAATGTTGTTCTGACTCGTTCTGGGAGTTTTGATATTGCTACTGCAGAGAATGTCGTCATATGTGGGAGCATGACCTCTGCTCTGGAGCTGTTAGCTGCTTCTCCTTACTGTCTGTCAATTGACAAAGTGTTTCTCATAGGAGGTGGCCAGATATTGAGGGAAGCTCTCAATGGGCCTGGCTGTGATGCAATTCACATTACAGAAATAGAGACCAACATTGAATGCGATACTTTTATCCCTGCAATTGATTCTACTGTATTTCAGCCATGGTATTCATCTTTTCCCAAGGTGGAGAATAGCATTCGATATTCCTTCACGACTTATGTGCGTGTGAGGAGTTCTGAAGTTGGATCCCTTTGTGAAAACAATGATCTGAACTGTAATAGTAGGTCGGATTCCATTAAGTGTGAGGTAAAGAAGTTCTCTTTCCTCCCCAAGATGATTTTCGAGAAACATGAGGAGTACCTGTATCTAAATCTGGTTCAAGATATACTCTCAGATGGTACTTCTAAGGATGATAGGACCGGGACTGGTACTTTATCAAAATTTGGTTGCCAGGTCCTTCAGGAGAAAGGCATCCGCATATGGGATGGAAATGCATCTAGAGACTACCTTGATAGGCAAGTATTGGACTTCCCACATGTAGGTGTTTGTGTCTATGATGAGGAGGAGGGCGACTTAGGACCAGTTTATGGGTTCCAGTGGCGACACTTTGGTGCCAGGTATACTGACATGCATGCTGACTACACTGGCCAAGGATTTGACCAGTTGTTAGATGTTATTGACAAGATCAAAAATAATCCTGATGATCGACGGATTATACTTTCAGCCTGGAATCCTTCTGATCTGAAATTGATGGCACTTCCACCTTGTCACATGTTTGCTCAGTTCTATGTTGCAAAAGGAGAGTTATCATGCCAAATGTATCAGCGTTCTGCTGACATGGGCCTGGGCGTGCCATTTAACATCGCGTCATATGCACTTCTGACATGCATGATTGCTCATGTCTGTGATCTTGTTCCAGGTGATTTCGTTCACGTTTTAGGGGATGCTCATGTTTACCGCACTCATATCAGGCCTCTGCAGGAGCAACTTCAGAAACTGCCCAAGCCTCTTCCAATTTTGAAGATCAACCCCGATAAAAAGAATATAGATTCATTTGTGGCAGCTGATTTCGAACTCATAGGTTATAATCCCCATGAGAAGATAGAAATGAAAATGGCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004146 GO:0004799 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0009058 GO:0009108 GO:0009256 GO:0009257 GO:0009396 GO:0009987 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016740 GO:0016741 GO:0018130 GO:0019438 GO:0019752 GO:0032259 GO:0034641 GO:0042083 GO:0042398 GO:0042558 GO:0042559 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko00670 ko00790 ko01100 map00240 map00670 map00790 map01100

Pfam Domains

Protein Families

DHFR_1 Thymidylat_synt

Protein Analysis

501

Amino Acids

55.97

Weight (kDa)

5.61

Isoelectric Point (pI)

38.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHFR_1	PF00186	27 - 202	1.5e-37	Dihydrofolate reductase
Thymidylat_synt	PF00303	278 - 501	4.1e-83	Thymidylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16370 AT2G16370 AT2G16370 AT4G34570 AT4G34570 AT4G34570 AT4G34570
fragaria_vesca	FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370
malus_domestica	MD05G1063200.v1.1 MD10G1070700.v1.1
prunus_persica	Prupe.8G092500_v2.0.a1 Prupe.8G092500_v2.0.a1
pyrus_communis	pycom05g05460 pycom05g05470 pycom10g05650
rosa_chinensis	RchiOBHm_Chr6g0265641
rosa_laevigata	RLG00000014170
rosa_multiflora	Rmu_sc0003233.1_g000014 Rmu_sc0003233.1_g000015 Rmu_sc0009907.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432460 Rroxscaffold_6G00388530 Rroxscaffold_7G00202550
rosa_rugosa	Rorug06G0018100
rosa_samantha	Rh1BG086900 Rh3BG370300 Rh3CG367200 Rh6AG140100 Rh6BG140000 Rh6CG137100 Rh6DG123400
rosa_wichuraiana	Rw6G012050 Rw6G012100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	215, 1467
AccB1I	GGYRCC	1 cut(s)	992
AccB7I	CCANNNNNTGG	1 cut(s)	968
AccI	GTMKAC	1 cut(s)	1001
AccII	CGCG	1 cut(s)	1247
AciI	CCGC	2 cut(s)	856, 1339
AclWI	GGATC	3 cut(s)	185, 621, 634
AcoI	YGGCCR	2 cut(s)	421, 1027
AcsI	RAATTY	1 cut(s)	821
AcuI	CTGAAG	3 cut(s)	639, 824, 1355
AcyI	GRCGYC	1 cut(s)	271
AfaI	GTAC	3 cut(s)	740, 781, 811
AfiI	CCNNNNNNNGG	4 cut(s)	266, 862, 920, 968
AflIII	ACRYGT	2 cut(s)	916, 1152
AgsI	TTSAA	3 cut(s)	497, 761, 1402
AjnI	CCWGG	8 cut(s)	193, 265, 451, 831, 995, 1106, 1224, 1297
AjuI	GAANNNNNNNTTGG	4 cut(s)	96, 128, 704, 736
AleI	CACNNNNGTG	1 cut(s)	990
AluBI	AGCT	5 cut(s)	58, 364, 371, 440, 1445
AluI	AGCT	5 cut(s)	58, 364, 371, 440, 1445
Alw26I	GTCTC	2 cut(s)	479, 876
AlwI	GGATC	3 cut(s)	185, 621, 634
AlwNI	CAGNNNCTG	2 cut(s)	194, 1378
Ama87I	CYCGRG	1 cut(s)	99
AoxI	GGCC	6 cut(s)	257, 421, 449, 1027, 1222, 1352
ApeKI	GCWGC	3 cut(s)	92, 371, 1442
ApoI	RAATTY	1 cut(s)	821
AspS9I	GGNCC	5 cut(s)	449, 798, 835, 959, 1222
AsuC2I	CCSGG	1 cut(s)	802
AsuHPI	GGTGA	2 cut(s)	20, 1313
AsuII	TTCGAA	1 cut(s)	1452
AvaI	CYCGRG	1 cut(s)	99
AvaII	GGWCC	3 cut(s)	798, 835, 959
BalI	TGGCCA	2 cut(s)	423, 1029
BamHI	GGATCC	1 cut(s)	626
BanI	GGYRCC	1 cut(s)	992
BbvI	GCAGC	3 cut(s)	79, 358, 1454
BccI	CCATC	4 cut(s)	116, 770, 860, 1126
BcgI	CGANNNNNNTGC	2 cut(s)	583, 617
BciT130I	CCWGG	8 cut(s)	195, 267, 453, 833, 997, 1108, 1226, 1299
BcnI	CCSGG	1 cut(s)	802
BcoDI	GTCTC	2 cut(s)	479, 876
BfaI	CTAG	1 cut(s)	879
BfmI	CTRYAG	2 cut(s)	318, 1358
BisI	GCNGC	3 cut(s)	93, 372, 1443
BlsI	GCNGC	3 cut(s)	94, 373, 1444
Bme1390I	CCNGG	9 cut(s)	195, 267, 453, 802, 833, 997, 1108, 1226, 1299
Bme18I	GGWCC	3 cut(s)	798, 835, 959
BmeT110I	CYCGRG	1 cut(s)	99
BmgT120I	GGNCC	5 cut(s)	449, 798, 835, 959, 1222
BmiI	GGNNCC	3 cut(s)	628, 974, 994
BmrFI	CCNGG	9 cut(s)	195, 267, 453, 802, 833, 997, 1108, 1226, 1299
BmsI	GCATC	5 cut(s)	262, 451, 861, 884, 1315
BplI	GAGNNNNNCTC	2 cut(s)	424, 456
BpmI	CTGGAG	1 cut(s)	380
Bpu10I	CCTNAGC	1 cut(s)	249
Bpu14I	TTCGAA	1 cut(s)	1452
BpuEI	CTTGAG	1 cut(s)	140
BpuMI	CCSGG	1 cut(s)	802
BsaHI	GRCGYC	1 cut(s)	271
BsaI	GGTCTC	1 cut(s)	479
BsaJI	CCNNGG	5 cut(s)	134, 542, 561, 1030, 1225
BsaXI	ACNNNNNCTCC	2 cut(s)	728, 758
Bsc4I	CCNNNNNNNGG	4 cut(s)	266, 862, 920, 968
Bse1I	ACTGG	5 cut(s)	811, 962, 976, 1030, 1042
Bse3DI	GCAATG	2 cut(s)	43, 49
BseBI	CCWGG	8 cut(s)	195, 267, 453, 833, 997, 1108, 1226, 1299
BseDI	CCNNGG	5 cut(s)	134, 542, 561, 1030, 1225
BseGI	GGATG	6 cut(s)	127, 175, 796, 852, 871, 1330
BseLI	CCNNNNNNNGG	4 cut(s)	266, 862, 920, 968
BseMI	GCAATG	2 cut(s)	43, 49
BseMII	CTCAG	3 cut(s)	240, 786, 1175
BseNI	ACTGG	5 cut(s)	811, 962, 976, 1030, 1042
BseRI	GAGGAG	4 cut(s)	625, 749, 956, 959
BseXI	GCAGC	3 cut(s)	79, 358, 1454
BseYI	CCCAGC	1 cut(s)	54
Bsh1236I	CGCG	1 cut(s)	1247
BshFI	GGCC	6 cut(s)	259, 423, 451, 1029, 1224, 1354
BshNI	GGYRCC	1 cut(s)	992
BsiHKCI	CYCGRG	1 cut(s)	99
BsiSI	CCGG	1 cut(s)	801
BslFI	GGGAC	1 cut(s)	817
BslI	CCNNNNNNNGG	4 cut(s)	266, 862, 920, 968
BsmAI	GTCTC	2 cut(s)	479, 876
BsmFI	GGGAC	1 cut(s)	817
BsmI	GAATGC	4 cut(s)	211, 240, 503, 576
BsnI	GGCC	6 cut(s)	259, 423, 451, 1029, 1224, 1354
Bso31I	GGTCTC	1 cut(s)	479
BsoBI	CYCGRG	1 cut(s)	99
Bsp119I	TTCGAA	1 cut(s)	1452
Bsp143I	GATC	9 cut(s)	151, 190, 626, 646, 1065, 1084, 1120, 1288, 1404
Bsp19I	CCATGG	1 cut(s)	542
BspACI	CCGC	2 cut(s)	856, 1339
BspANI	GGCC	6 cut(s)	259, 423, 451, 1029, 1224, 1354
BspCNI	CTCAG	3 cut(s)	241, 785, 1174
BspFNI	CGCG	1 cut(s)	1247
BspLI	GGNNCC	3 cut(s)	628, 974, 994
BspMAI	CTGCAG	2 cut(s)	322, 1362
BspPI	GGATC	3 cut(s)	185, 621, 634
BspT104I	TTCGAA	1 cut(s)	1452
BspT107I	GGYRCC	1 cut(s)	992
BspTNI	GGTCTC	1 cut(s)	479
BsrDI	GCAATG	2 cut(s)	43, 49
BsrI	ACTGG	5 cut(s)	811, 962, 976, 1030, 1042
BssECI	CCNNGG	5 cut(s)	134, 542, 561, 1030, 1225
BssMI	GATC	9 cut(s)	151, 190, 626, 646, 1065, 1084, 1120, 1288, 1404
BssNAI	GTATAC	1 cut(s)	1002
BssNI	GRCGYC	1 cut(s)	271
BssT1I	CCWWGG	4 cut(s)	134, 542, 561, 1030
Bst1107I	GTATAC	1 cut(s)	1002
Bst2UI	CCWGG	8 cut(s)	195, 267, 453, 833, 997, 1108, 1226, 1299
Bst4CI	ACNGT	5 cut(s)	22, 178, 386, 532, 656
Bst6I	CTCTTC	1 cut(s)	1395
BstACI	GRCGYC	1 cut(s)	271
BstBI	TTCGAA	1 cut(s)	1452
BstC8I	GCNNGC	2 cut(s)	1014, 1232
BstDEI	CTNAG	5 cut(s)	249, 772, 786, 955, 1161
BstDSI	CCRYGG	1 cut(s)	542
BstF5I	GGATG	6 cut(s)	127, 175, 796, 852, 871, 1330
BstFNI	CGCG	1 cut(s)	1247
BstKTI	GATC	9 cut(s)	154, 193, 629, 649, 1068, 1087, 1123, 1291, 1407
BstMAI	GTCTC	2 cut(s)	479, 876
BstMBI	GATC	9 cut(s)	151, 190, 626, 646, 1065, 1084, 1120, 1288, 1404
BstMWI	GCNNNNNNNGC	1 cut(s)	1253
BstNI	CCWGG	8 cut(s)	195, 267, 453, 833, 997, 1108, 1226, 1299
BstNSI	RCATGY	5 cut(s)	920, 1012, 1016, 1156, 1269
BstSCI	CCNGG	9 cut(s)	193, 265, 451, 800, 831, 995, 1106, 1224, 1297
BstSFI	CTRYAG	2 cut(s)	318, 1358
BstUI	CGCG	1 cut(s)	1247
BstV1I	GCAGC	3 cut(s)	79, 358, 1454
BstX2I	RGATCY	2 cut(s)	190, 626
BstYI	RGATCY	2 cut(s)	190, 626
BstZ17I	GTATAC	1 cut(s)	1002
BsuRI	GGCC	6 cut(s)	259, 423, 451, 1029, 1224, 1354
BtgI	CCRYGG	1 cut(s)	542
BtgZI	GCGATG	1 cut(s)	1228
BtsCI	GGATG	6 cut(s)	127, 175, 796, 852, 871, 1330
BtsIMutI	CAGTG	3 cut(s)	174, 983, 1023
Cac8I	GCNNGC	2 cut(s)	1014, 1232
CaiI	CAGNNNCTG	2 cut(s)	194, 1378
Cfr13I	GGNCC	5 cut(s)	449, 798, 835, 959, 1222
CseI	GACGC	2 cut(s)	279, 1236
Csp6I	GTAC	3 cut(s)	739, 780, 810
CviQI	GTAC	3 cut(s)	739, 780, 810
DdeI	CTNAG	5 cut(s)	249, 772, 786, 955, 1161
DpnI	GATC	9 cut(s)	153, 192, 628, 648, 1067, 1086, 1122, 1290, 1406
DpnII	GATC	9 cut(s)	151, 190, 626, 646, 1065, 1084, 1120, 1288, 1404
EaeI	YGGCCR	2 cut(s)	421, 1027
Eam1104I	CTCTTC	1 cut(s)	1395
EarI	CTCTTC	1 cut(s)	1395
Eco130I	CCWWGG	4 cut(s)	134, 542, 561, 1030
Eco147I	AGGCCT	1 cut(s)	1354
Eco31I	GGTCTC	1 cut(s)	479
Eco47I	GGWCC	3 cut(s)	798, 835, 959
Eco57I	CTGAAG	3 cut(s)	639, 824, 1355
Eco88I	CYCGRG	1 cut(s)	99
EcoO109I	RGGNCCY	1 cut(s)	835
EcoRII	CCWGG	8 cut(s)	193, 265, 451, 831, 995, 1106, 1224, 1297
EcoT14I	CCWWGG	4 cut(s)	134, 542, 561, 1030
EcoT22I	ATGCAT	3 cut(s)	877, 1014, 1271
ErhI	CCWWGG	4 cut(s)	134, 542, 561, 1030
FaqI	GGGAC	1 cut(s)	817
FauNDI	CATATG	3 cut(s)	335, 860, 1252
FblI	GTMKAC	1 cut(s)	1001
Fnu4HI	GCNGC	3 cut(s)	93, 372, 1443
FokI	GGATG	6 cut(s)	134, 182, 803, 839, 878, 1337
Fsp4HI	GCNGC	3 cut(s)	93, 372, 1443
FspBI	CTAG	1 cut(s)	879
GluI	GCNGC	3 cut(s)	93, 372, 1443
GsaI	CCCAGC	1 cut(s)	58
GsuI	CTGGAG	1 cut(s)	380
HaeIII	GGCC	6 cut(s)	259, 423, 451, 1029, 1224, 1354
HapII	CCGG	1 cut(s)	801
HgaI	GACGC	2 cut(s)	279, 1236
Hin1I	GRCGYC	1 cut(s)	271
HinfI	GANTC	6 cut(s)	61, 289, 524, 671, 1111, 1430
HpaII	CCGG	1 cut(s)	801
HphI	GGTGA	2 cut(s)	20, 1313
Hpy166II	GTNNAC	3 cut(s)	1002, 1312, 1336
Hpy188III	TCNNGA	8 cut(s)	101, 359, 592, 724, 761, 842, 879, 1079
Hpy8I	GTNNAC	3 cut(s)	1002, 1312, 1336
Hpy99I	CGWCG	1 cut(s)	1092
HpyAV	CCTTC	4 cut(s)	156, 598, 848, 1125
HpyCH4III	ACNGT	5 cut(s)	22, 178, 386, 532, 656
HpyCH4IV	ACGT	1 cut(s)	1314
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1253
HpyF3I	CTNAG	5 cut(s)	249, 772, 786, 955, 1161
HpySE526I	ACGT	1 cut(s)	1314
Hsp92I	GRCGYC	1 cut(s)	271
Kzo9I	GATC	9 cut(s)	151, 190, 626, 646, 1065, 1084, 1120, 1288, 1404
LmnI	GCTCC	3 cut(s)	342, 361, 1363
Lsp1109I	GCAGC	3 cut(s)	79, 358, 1454
LweI	GCATC	5 cut(s)	262, 451, 861, 884, 1315
MaeI	CTAG	1 cut(s)	879
MaeII	ACGT	1 cut(s)	1314
MaeIII	GTNAC	4 cut(s)	16, 172, 178, 1148
MalI	GATC	9 cut(s)	153, 192, 628, 648, 1067, 1086, 1122, 1290, 1406
MboI	GATC	9 cut(s)	151, 190, 626, 646, 1065, 1084, 1120, 1288, 1404
MboII	GAAGA	3 cut(s)	1382, 1414, 1492
MfeI	CAATTG	2 cut(s)	393, 519
MflI	RGATCY	2 cut(s)	190, 626
MlsI	TGGCCA	2 cut(s)	423, 1029
MluCI	AATT	7 cut(s)	210, 393, 465, 519, 821, 1127, 1395
MluNI	TGGCCA	2 cut(s)	423, 1029
MlyI	GAGTC	1 cut(s)	283
MmeI	TCCRAC	2 cut(s)	604, 648
Mox20I	TGGCCA	2 cut(s)	423, 1029
Mph1103I	ATGCAT	3 cut(s)	877, 1014, 1271
MscI	TGGCCA	2 cut(s)	423, 1029
MseI	TTAA	4 cut(s)	165, 276, 678, 1239
MslI	CAYNNNNRTG	3 cut(s)	921, 990, 1284
Msp20I	TGGCCA	2 cut(s)	423, 1029
MspA1I	CMGCKG	2 cut(s)	58, 1445
MspI	CCGG	1 cut(s)	801
MspR9I	CCNGG	9 cut(s)	195, 267, 453, 802, 833, 997, 1108, 1226, 1299
MunI	CAATTG	2 cut(s)	393, 519
Mva1269I	GAATGC	4 cut(s)	211, 240, 503, 576
MvaI	CCWGG	8 cut(s)	195, 267, 453, 833, 997, 1108, 1226, 1299
MvnI	CGCG	1 cut(s)	1247
MwoI	GCNNNNNNNGC	1 cut(s)	1253
NciI	CCSGG	1 cut(s)	802
NcoI	CCATGG	1 cut(s)	542
NdeI	CATATG	3 cut(s)	335, 860, 1252
NdeII	GATC	9 cut(s)	151, 190, 626, 646, 1065, 1084, 1120, 1288, 1404
NlaIV	GGNNCC	3 cut(s)	628, 974, 994
NmuCI	GTSAC	3 cut(s)	16, 172, 1148
NsiI	ATGCAT	3 cut(s)	877, 1014, 1271
NspI	RCATGY	5 cut(s)	920, 1012, 1016, 1156, 1269
NspV	TTCGAA	1 cut(s)	1452
OliI	CACNNNNGTG	1 cut(s)	990
PaeI	GCATGC	1 cut(s)	1016
PaeR7I	CTCGAG	1 cut(s)	99
PceI	AGGCCT	1 cut(s)	1354
PciI	ACATGT	2 cut(s)	916, 1152
PctI	GAATGC	4 cut(s)	211, 240, 503, 576
PfeI	GAWTC	5 cut(s)	61, 524, 671, 1111, 1430
PflMI	CCANNNNNTGG	1 cut(s)	968
PfoI	TCCNGGA	1 cut(s)	193
PkrI	GCNGC	3 cut(s)	94, 373, 1444
PleI	GAGTC	1 cut(s)	283
PpsI	GAGTC	1 cut(s)	283
PpuMI	RGGWCCY	1 cut(s)	835
PscI	ACATGT	2 cut(s)	916, 1152
PsiI	TTATAA	2 cut(s)	215, 1467
Psp5II	RGGWCCY	1 cut(s)	835
Psp6I	CCWGG	8 cut(s)	193, 265, 451, 831, 995, 1106, 1224, 1297
PspFI	CCCAGC	1 cut(s)	54
PspGI	CCWGG	8 cut(s)	193, 265, 451, 831, 995, 1106, 1224, 1297
PspN4I	GGNNCC	3 cut(s)	628, 974, 994
PspPI	GGNCC	5 cut(s)	449, 798, 835, 959, 1222
PspPPI	RGGWCCY	1 cut(s)	835
PstI	CTGCAG	2 cut(s)	322, 1362
PstNI	CAGNNNCTG	2 cut(s)	194, 1378
PsuI	RGATCY	2 cut(s)	190, 626
PvuII	CAGCTG	2 cut(s)	58, 1445
RsaI	GTAC	3 cut(s)	740, 781, 811
RsaNI	GTAC	3 cut(s)	739, 780, 810
RseI	CAYNNNNRTG	3 cut(s)	921, 990, 1284
SaqAI	TTAA	4 cut(s)	165, 276, 678, 1239
SatI	GCNGC	3 cut(s)	93, 372, 1443
Sau3AI	GATC	9 cut(s)	151, 190, 626, 646, 1065, 1084, 1120, 1288, 1404
Sau96I	GGNCC	5 cut(s)	449, 798, 835, 959, 1222
SchI	GAGTC	1 cut(s)	283
ScrFI	CCNGG	9 cut(s)	195, 267, 453, 802, 833, 997, 1108, 1226, 1299
SfaNI	GCATC	5 cut(s)	262, 451, 861, 884, 1315
SfcI	CTRYAG	2 cut(s)	318, 1358
Sfr274I	CTCGAG	1 cut(s)	99
SfuI	TTCGAA	1 cut(s)	1452
SinI	GGWCC	3 cut(s)	798, 835, 959
SlaI	CTCGAG	1 cut(s)	99
SmiMI	CAYNNNNRTG	3 cut(s)	921, 990, 1284
SmlI	CTYRAG	2 cut(s)	99, 155
SmoI	CTYRAG	2 cut(s)	99, 155
SphI	GCATGC	1 cut(s)	1016
Sse9I	AATT	7 cut(s)	210, 393, 465, 519, 821, 1127, 1395
SseBI	AGGCCT	1 cut(s)	1354
SsiI	CCGC	2 cut(s)	856, 1339
SspMI	CTAG	1 cut(s)	879
StuI	AGGCCT	1 cut(s)	1354
StyD4I	CCNGG	9 cut(s)	193, 265, 451, 800, 831, 995, 1106, 1224, 1297
StyI	CCWWGG	4 cut(s)	134, 542, 561, 1030
TaaI	ACNGT	5 cut(s)	22, 178, 386, 532, 656
TaiI	ACGT	1 cut(s)	1317
TaqI	TCGA	5 cut(s)	100, 580, 723, 1087, 1452
TasI	AATT	7 cut(s)	210, 393, 465, 519, 821, 1127, 1395
TfiI	GAWTC	5 cut(s)	61, 524, 671, 1111, 1430
Tru1I	TTAA	4 cut(s)	165, 276, 678, 1239
Tru9I	TTAA	4 cut(s)	165, 276, 678, 1239
TscAI	CASTG	3 cut(s)	181, 983, 1030
TseFI	GTSAC	3 cut(s)	16, 172, 1148
TseI	GCWGC	3 cut(s)	92, 371, 1442
Tsp45I	GTSAC	3 cut(s)	16, 172, 1148
TspDTI	ATGAA	3 cut(s)	540, 1422, 1505
TspGWI	ACGGA	1 cut(s)	1105
TspRI	CASTG	3 cut(s)	181, 983, 1030
Van91I	CCANNNNNTGG	1 cut(s)	968
VpaK11BI	GGWCC	3 cut(s)	798, 835, 959
XapI	RAATTY	1 cut(s)	821
XbaI	TCTAGA	1 cut(s)	878
XceI	RCATGY	5 cut(s)	920, 1012, 1016, 1156, 1269
XhoI	CTCGAG	1 cut(s)	99
XmiI	GTMKAC	1 cut(s)	1001
XspI	CTAG	1 cut(s)	879
Zsp2I	ATGCAT	3 cut(s)	877, 1014, 1271

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.