Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002969

Physical Location & Seq

Genomic Coordinates Forward (+)

9812672 .. 9817315

Gene Structure 4644 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1070700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGGCTGGTGAATTGTTGGTGAGTGCGAATGGAAATGGTAACACCCAGCCCAATCCACAGAGGACTTACCAAGTTGTTGTGGCTGCGACCCGAGAAATGGGTATTGGAAAGGATGGGAAACTACCTTGGAGACTGCCCTCTGATCTCAAGTTCTTTAAGGACGTCACTGTGACCACATCAGATCCTGCGAAAAAGAATGCGATTATAATGGGTAGAAAAACATGGGAGAGCATTCCATCTGAGCATCAACCTCTACCTGGCCGCCTTAATGTTGTTCTGACTCGTTCTGGGAGTTTTGATATTGCTACTGCAGAGAATGTTGTGATATGCGGAAGCATGGCATCTGCTTTGGAATTGTTAGCTGCTTCTCCTTACTGTCTGTCAATTGAGAGAGTGTTTCTCATTGGAGGTGGCCAGATATTAAGGGAAGCTCTCAATGCGCCTGGCTGTGAAGCCATTCACATTACAGAAATAGAGACAAACATTGAATGTGACACTTTTATCCCTGCAATTGATTCCTCTGTGTTTCAGCCGTGGTATTCATCCTTTCCCAAGGTTGAAAATAGCATTCGTCATTCCTTCACAACTTATGTTCGTGTAAGGAGTTCTGCAGTTGAATCCCTTTGTGAAAACAATGATCTGATCTCTAATAGTAAGTCAGGCTCCATTAAATGTGAGGTGAAGAATTTCTCTTTTCTTCCAAAGATGATTTTTGAGAAACATGAGGAGCACATGTATCTAAGGCTGGTACGAGAAGTACTCTCAGATGGCACTTCTAAGGATGACAGGACAGGGACTGGTACTTTGTCAAAATTCGGTTGCCAGATGCGGTTTAACCTGCGCAGAACTTTTCCACTTCTGACAACCAAGAAAGTATTTTGGCGAGGGGTTGTTGAAGAACTTCTGTGGTTCATCAGTGGTTCAACAAATGCCAAGGTCCTTCAGGAGAAAGGTATTCACATTTGGGATGGAAATGCATCTAGAGACTACCTTGACAGTGTTGGTTTAACTGACAAGGAAGAGGGCGACTTGGGACCAGTATATGGGTTCCAGTGGCGACACTTTGGTGCCAGGTATACTGACATGCATGCTGACTATACCGGTCAAGGATTTGATCAGTTGCTAGATGTTATTGACAAGATTAAAAATAAGCCAGATGATCGGCGGATTATACTGTCAGCATGGAATCCTTCTGATCTCAAATTGATGGCACTCCCACCTTGTCACATGTTTGCTCAGTTCTATGTAGCCAACGGAGAGTTATCATGTCAAATGTATCAGCGTTCTGCTGACCTCGGCCTTGGTGTTCCATTTAACATTGCATCATATGCTCTTCTGACATGCATGATTGCTCATGTTTGTGGTCTTGTTCCAGGTGATTTCATTCATGCCTTAGGGGATGCTCATGTTTACCGCACTCACATCAGGCCTTTGAAGGAGCAGCTTGAAAAGCTGCCCAAGCCTTTTCCAATTTTGAAGATCAATCCTGAGAAAAAGAATATAGATTCGTTTGTGGCGGCTGATTTCAAACTGATAGGTTATGATCCTCATCAGAAGATAGAAATGAAAATGGCCGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004146 GO:0004799 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0009058 GO:0009108 GO:0009256 GO:0009257 GO:0009396 GO:0009987 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016740 GO:0016741 GO:0018130 GO:0019438 GO:0019752 GO:0032259 GO:0034641 GO:0042083 GO:0042398 GO:0042558 GO:0042559 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko00670 ko00790 ko01100 map00240 map00670 map00790 map01100

Pfam Domains

Protein Families

DHFR_1 Thymidylat_synt

Protein Analysis

527

Amino Acids

58.86

Weight (kDa)

6.92

Isoelectric Point (pI)

42.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHFR_1	PF00186	24 - 199	1.5e-36	Dihydrofolate reductase
Thymidylat_synt	PF00303	245 - 526	8e-116	Thymidylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16370 AT2G16370 AT2G16370 AT4G34570 AT4G34570 AT4G34570 AT4G34570
fragaria_vesca	FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370
malus_domestica	MD05G1063200.v1.1 MD10G1070700.v1.1
prunus_persica	Prupe.8G092500_v2.0.a1 Prupe.8G092500_v2.0.a1
pyrus_communis	pycom05g05460 pycom05g05470 pycom10g05650
rosa_chinensis	RchiOBHm_Chr6g0265641
rosa_laevigata	RLG00000014170
rosa_multiflora	Rmu_sc0003233.1_g000014 Rmu_sc0003233.1_g000015 Rmu_sc0009907.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432460 Rroxscaffold_6G00388530 Rroxscaffold_7G00202550
rosa_rugosa	Rorug06G0018100
rosa_samantha	Rh1BG086900 Rh3BG370300 Rh3CG367200 Rh6AG140100 Rh6BG140000 Rh6CG137100 Rh6DG123400
rosa_wichuraiana	Rw6G012050 Rw6G012100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	206
AatII	GACGTC	1 cut(s)	165
Acc16I	TGCGCA	1 cut(s)	842
Acc36I	ACCTGC	1 cut(s)	846
AccB1I	GGYRCC	1 cut(s)	1067
AccB7I	CCANNNNNTGG	1 cut(s)	1043
AccI	GTMKAC	1 cut(s)	1076
AciI	CCGC	6 cut(s)	262, 330, 829, 1165, 1414, 1517
AclWI	GGATC	2 cut(s)	176, 1538
AcoI	YGGCCR	3 cut(s)	259, 412, 1572
AcsI	RAATTY	2 cut(s)	685, 812
AcuI	CTGAAG	1 cut(s)	926
AcyI	GRCGYC	1 cut(s)	162
AfaI	GTAC	3 cut(s)	750, 759, 802
AfiI	CCNNNNNNNGG	3 cut(s)	97, 257, 1043
AflIII	ACRYGT	2 cut(s)	732, 1227
AgeI	ACCGGT	1 cut(s)	1100
AgsI	TTSAA	9 cut(s)	488, 560, 617, 896, 924, 1435, 1448, 1477, 1528
AjnI	CCWGG	4 cut(s)	256, 442, 1070, 1372
AjuI	GAANNNNNNNTTGG	2 cut(s)	87, 119
AleI	CACNNNNGTG	1 cut(s)	1065
AluBI	AGCT	4 cut(s)	362, 431, 1444, 1453
AluI	AGCT	4 cut(s)	362, 431, 1444, 1453
Alw21I	GWGCWC	1 cut(s)	732
Alw26I	GTCTC	3 cut(s)	124, 470, 978
AlwI	GGATC	2 cut(s)	176, 1538
AlwNI	CAGNNNCTG	2 cut(s)	185, 797
Ama87I	CYCGRG	1 cut(s)	90
AoxI	GGCC	5 cut(s)	259, 412, 1297, 1427, 1572
ApeKI	GCWGC	4 cut(s)	83, 362, 1441, 1453
ApoI	RAATTY	2 cut(s)	685, 812
ArsI	GACNNNNNNTTYG	2 cut(s)	787, 819
AsiGI	ACCGGT	1 cut(s)	1100
Asp700I	GAANNNNTTC	3 cut(s)	456, 686, 900
AspLEI	GCGC	2 cut(s)	442, 843
AspS9I	GGNCC	2 cut(s)	937, 1034
AsuHPI	GGTGA	4 cut(s)	20, 31, 691, 1388
AvaI	CYCGRG	1 cut(s)	90
AvaII	GGWCC	2 cut(s)	937, 1034
AxyI	CCTNAGG	1 cut(s)	1393
BalI	TGGCCA	1 cut(s)	414
BanI	GGYRCC	1 cut(s)	1067
Bbv12I	GWGCWC	1 cut(s)	732
BbvI	GCAGC	4 cut(s)	70, 349, 1440, 1453
BccI	CCATC	5 cut(s)	107, 244, 761, 962, 1201
BceAI	ACGGC	2 cut(s)	517, 1559
BciT130I	CCWGG	4 cut(s)	258, 444, 1072, 1374
BclI	TGATCA	1 cut(s)	1114
BcoDI	GTCTC	3 cut(s)	124, 470, 978
BfaI	CTAG	2 cut(s)	981, 1124
BfmI	CTRYAG	2 cut(s)	309, 609
BfuAI	ACCTGC	1 cut(s)	846
BisI	GCNGC	6 cut(s)	84, 262, 363, 1442, 1454, 1518
BlsI	GCNGC	6 cut(s)	85, 263, 364, 1443, 1455, 1519
BmcAI	AGTACT	1 cut(s)	759
Bme1390I	CCNGG	4 cut(s)	258, 444, 1072, 1374
Bme18I	GGWCC	2 cut(s)	937, 1034
BmeT110I	CYCGRG	1 cut(s)	90
BmgT120I	GGNCC	2 cut(s)	937, 1034
BmiI	GGNNCC	4 cut(s)	664, 1035, 1049, 1069
BmrFI	CCNGG	4 cut(s)	258, 444, 1072, 1374
BmsI	GCATC	6 cut(s)	253, 350, 816, 986, 1331, 1390
BplI	GAGNNNNNCTC	4 cut(s)	384, 416, 744, 776
BpuEI	CTTGAG	1 cut(s)	131
BsaBI	GATNNNNATC	1 cut(s)	1548
BsaHI	GRCGYC	1 cut(s)	162
BsaJI	CCNNGG	6 cut(s)	125, 533, 552, 933, 1294, 1300
BsaWI	WCCGGW	1 cut(s)	1100
BsaXI	ACNNNNNCTCC	2 cut(s)	719, 749
Bsc4I	CCNNNNNNNGG	3 cut(s)	97, 257, 1043
Bse118I	RCCGGY	1 cut(s)	1100
Bse1I	ACTGG	3 cut(s)	802, 1037, 1051
Bse21I	CCTNAGG	1 cut(s)	1393
Bse3DI	GCAATG	1 cut(s)	1317
Bse8I	GATNNNNATC	1 cut(s)	1548
BseBI	CCWGG	4 cut(s)	258, 444, 1072, 1374
BseDI	CCNNGG	6 cut(s)	125, 533, 552, 933, 1294, 1300
BseGI	GGATG	5 cut(s)	118, 542, 787, 973, 1405
BseJI	GATNNNNATC	1 cut(s)	1548
BseLI	CCNNNNNNNGG	3 cut(s)	97, 257, 1043
BseMI	GCAATG	1 cut(s)	1317
BseMII	CTCAG	4 cut(s)	231, 777, 1250, 1479
BseNI	ACTGG	3 cut(s)	802, 1037, 1051
BseRI	GAGGAG	1 cut(s)	740
BseXI	GCAGC	4 cut(s)	70, 349, 1440, 1453
BseYI	CCCAGC	1 cut(s)	45
BshFI	GGCC	5 cut(s)	261, 414, 1299, 1429, 1574
BshNI	GGYRCC	1 cut(s)	1067
BshTI	ACCGGT	1 cut(s)	1100
BsiHKAI	GWGCWC	1 cut(s)	732
BsiHKCI	CYCGRG	1 cut(s)	90
BsiSI	CCGG	1 cut(s)	1101
BslFI	GGGAC	2 cut(s)	808, 1047
BslI	CCNNNNNNNGG	3 cut(s)	97, 257, 1043
BsmAI	GTCTC	3 cut(s)	124, 470, 978
BsmFI	GGGAC	2 cut(s)	808, 1047
BsmI	GAATGC	3 cut(s)	202, 231, 567
BsnI	GGCC	5 cut(s)	261, 414, 1299, 1429, 1574
BsoBI	CYCGRG	1 cut(s)	90
Bsp1286I	GDGCHC	1 cut(s)	732
Bsp143I	GATC	9 cut(s)	142, 181, 637, 642, 1114, 1159, 1195, 1479, 1543
BspACI	CCGC	6 cut(s)	262, 330, 829, 1165, 1414, 1517
BspANI	GGCC	5 cut(s)	261, 414, 1299, 1429, 1574
BspCNI	CTCAG	4 cut(s)	232, 776, 1249, 1480
BspLI	GGNNCC	4 cut(s)	664, 1035, 1049, 1069
BspMAI	CTGCAG	2 cut(s)	313, 613
BspMI	ACCTGC	1 cut(s)	846
BspPI	GGATC	2 cut(s)	176, 1538
BspQI	GCTCTTC	1 cut(s)	1338
BspT107I	GGYRCC	1 cut(s)	1067
BsrDI	GCAATG	1 cut(s)	1317
BsrFI	RCCGGY	1 cut(s)	1100
BsrI	ACTGG	3 cut(s)	802, 1037, 1051
BssAI	RCCGGY	1 cut(s)	1100
BssECI	CCNNGG	6 cut(s)	125, 533, 552, 933, 1294, 1300
BssMI	GATC	9 cut(s)	142, 181, 637, 642, 1114, 1159, 1195, 1479, 1543
BssNAI	GTATAC	1 cut(s)	1077
BssNI	GRCGYC	1 cut(s)	162
BssT1I	CCWWGG	4 cut(s)	125, 552, 933, 1300
Bst1107I	GTATAC	1 cut(s)	1077
Bst2UI	CCWGG	4 cut(s)	258, 444, 1072, 1374
Bst4CI	ACNGT	4 cut(s)	169, 377, 998, 1176
Bst6I	CTCTTC	2 cut(s)	1014, 1338
BstACI	GRCGYC	1 cut(s)	162
BstAPI	GCANNNNNTGC	1 cut(s)	1328
BstC8I	GCNNGC	1 cut(s)	1089
BstDEI	CTNAG	7 cut(s)	240, 740, 763, 777, 1236, 1393, 1488
BstDSI	CCRYGG	1 cut(s)	533
BstF5I	GGATG	5 cut(s)	118, 542, 787, 973, 1405
BstHHI	GCGC	2 cut(s)	442, 843
BstKTI	GATC	9 cut(s)	145, 184, 640, 645, 1117, 1162, 1198, 1482, 1546
BstMAI	GTCTC	3 cut(s)	124, 470, 978
BstMBI	GATC	9 cut(s)	142, 181, 637, 642, 1114, 1159, 1195, 1479, 1543
BstMWI	GCNNNNNNNGC	4 cut(s)	437, 1328, 1450, 1459
BstNI	CCWGG	4 cut(s)	258, 444, 1072, 1374
BstNSI	RCATGY	5 cut(s)	736, 1087, 1091, 1231, 1344
BstSCI	CCNGG	4 cut(s)	256, 442, 1070, 1372
BstSFI	CTRYAG	2 cut(s)	309, 609
BstV1I	GCAGC	4 cut(s)	70, 349, 1440, 1453
BstX2I	RGATCY	1 cut(s)	181
BstYI	RGATCY	1 cut(s)	181
BstZ17I	GTATAC	1 cut(s)	1077
Bsu36I	CCTNAGG	1 cut(s)	1393
BsuRI	GGCC	5 cut(s)	261, 414, 1299, 1429, 1574
BtgI	CCRYGG	1 cut(s)	533
BtsCI	GGATG	5 cut(s)	118, 542, 787, 973, 1405
BtsIMutI	CAGTG	4 cut(s)	165, 922, 1003, 1058
BveI	ACCTGC	1 cut(s)	846
Cac8I	GCNNGC	1 cut(s)	1089
CaiI	CAGNNNCTG	2 cut(s)	185, 797
CfoI	GCGC	2 cut(s)	442, 843
Cfr10I	RCCGGY	1 cut(s)	1100
Cfr13I	GGNCC	2 cut(s)	937, 1034
Csp6I	GTAC	3 cut(s)	749, 758, 801
CspAI	ACCGGT	1 cut(s)	1100
CviQI	GTAC	3 cut(s)	749, 758, 801
DdeI	CTNAG	7 cut(s)	240, 740, 763, 777, 1236, 1393, 1488
DpnI	GATC	9 cut(s)	144, 183, 639, 644, 1116, 1161, 1197, 1481, 1545
DpnII	GATC	9 cut(s)	142, 181, 637, 642, 1114, 1159, 1195, 1479, 1543
EaeI	YGGCCR	3 cut(s)	259, 412, 1572
Eam1104I	CTCTTC	2 cut(s)	1014, 1338
EarI	CTCTTC	2 cut(s)	1014, 1338
EciI	GGCGGA	1 cut(s)	1180
Eco130I	CCWWGG	4 cut(s)	125, 552, 933, 1300
Eco147I	AGGCCT	1 cut(s)	1429
Eco47I	GGWCC	2 cut(s)	937, 1034
Eco57I	CTGAAG	1 cut(s)	926
Eco81I	CCTNAGG	1 cut(s)	1393
Eco88I	CYCGRG	1 cut(s)	90
EcoO109I	RGGNCCY	1 cut(s)	937
EcoRII	CCWGG	4 cut(s)	256, 442, 1070, 1372
EcoT14I	CCWWGG	4 cut(s)	125, 552, 933, 1300
EcoT22I	ATGCAT	3 cut(s)	979, 1089, 1346
ErhI	CCWWGG	4 cut(s)	125, 552, 933, 1300
FaqI	GGGAC	2 cut(s)	808, 1047
FauNDI	CATATG	1 cut(s)	1327
FbaI	TGATCA	1 cut(s)	1114
FblI	GTMKAC	1 cut(s)	1076
Fnu4HI	GCNGC	6 cut(s)	84, 262, 363, 1442, 1454, 1518
FokI	GGATG	5 cut(s)	125, 529, 794, 980, 1412
Fsp4HI	GCNGC	6 cut(s)	84, 262, 363, 1442, 1454, 1518
FspBI	CTAG	2 cut(s)	981, 1124
FspI	TGCGCA	1 cut(s)	842
GlaI	GCGC	2 cut(s)	441, 842
GluI	GCNGC	6 cut(s)	84, 262, 363, 1442, 1454, 1518
GsaI	CCCAGC	1 cut(s)	49
HaeIII	GGCC	5 cut(s)	261, 414, 1299, 1429, 1574
HapII	CCGG	1 cut(s)	1101
HhaI	GCGC	2 cut(s)	442, 843
Hin1I	GRCGYC	1 cut(s)	162
Hin6I	GCGC	2 cut(s)	440, 841
HinP1I	GCGC	2 cut(s)	440, 841
HinfI	GANTC	5 cut(s)	280, 515, 617, 1186, 1505
HpaII	CCGG	1 cut(s)	1101
HphI	GGTGA	4 cut(s)	20, 31, 691, 1388
Hpy166II	GTNNAC	2 cut(s)	1077, 1411
Hpy188III	TCNNGA	3 cut(s)	944, 981, 1487
Hpy8I	GTNNAC	2 cut(s)	1077, 1411
HpyAV	CCTTC	4 cut(s)	589, 950, 1200, 1429
HpyCH4III	ACNGT	4 cut(s)	169, 377, 998, 1176
HpyCH4IV	ACGT	1 cut(s)	162
HpyCH4V	TGCA	7 cut(s)	311, 509, 611, 977, 1087, 1322, 1344
HpyF10VI	GCNNNNNNNGC	4 cut(s)	437, 1328, 1450, 1459
HpyF3I	CTNAG	7 cut(s)	240, 740, 763, 777, 1236, 1393, 1488
HpySE526I	ACGT	1 cut(s)	162
Hsp92I	GRCGYC	1 cut(s)	162
HspAI	GCGC	2 cut(s)	440, 841
Ksp22I	TGATCA	1 cut(s)	1114
Kzo9I	GATC	9 cut(s)	142, 181, 637, 642, 1114, 1159, 1195, 1479, 1543
LguI	GCTCTTC	1 cut(s)	1338
LmnI	GCTCC	3 cut(s)	668, 727, 1438
Lsp1109I	GCAGC	4 cut(s)	70, 349, 1440, 1453
LweI	GCATC	6 cut(s)	253, 350, 816, 986, 1331, 1390
MaeI	CTAG	2 cut(s)	981, 1124
MaeII	ACGT	1 cut(s)	162
MaeIII	GTNAC	5 cut(s)	38, 163, 169, 491, 1223
MalI	GATC	9 cut(s)	144, 183, 639, 644, 1116, 1161, 1197, 1481, 1545
MboI	GATC	9 cut(s)	142, 181, 637, 642, 1114, 1159, 1195, 1479, 1543
MboII	GAAGA	7 cut(s)	689, 694, 908, 1031, 1325, 1489, 1567
MfeI	CAATTG	2 cut(s)	384, 510
MflI	RGATCY	1 cut(s)	181
MhlI	GDGCHC	1 cut(s)	732
MlsI	TGGCCA	1 cut(s)	414
MluCI	AATT	8 cut(s)	11, 353, 384, 510, 685, 812, 1202, 1470
MluNI	TGGCCA	1 cut(s)	414
MlyI	GAGTC	1 cut(s)	274
Mox20I	TGGCCA	1 cut(s)	414
Mph1103I	ATGCAT	3 cut(s)	979, 1089, 1346
MroXI	GAANNNNTTC	3 cut(s)	456, 686, 900
MscI	TGGCCA	1 cut(s)	414
MseI	TTAA	8 cut(s)	156, 267, 422, 669, 834, 1007, 1143, 1314
MslI	CAYNNNNRTG	2 cut(s)	1065, 1359
Msp20I	TGGCCA	1 cut(s)	414
MspI	CCGG	1 cut(s)	1101
MspR9I	CCNGG	4 cut(s)	258, 444, 1072, 1374
MunI	CAATTG	2 cut(s)	384, 510
Mva1269I	GAATGC	3 cut(s)	202, 231, 567
MvaI	CCWGG	4 cut(s)	258, 444, 1072, 1374
MwoI	GCNNNNNNNGC	4 cut(s)	437, 1328, 1450, 1459
NdeI	CATATG	1 cut(s)	1327
NdeII	GATC	9 cut(s)	142, 181, 637, 642, 1114, 1159, 1195, 1479, 1543
NlaIV	GGNNCC	4 cut(s)	664, 1035, 1049, 1069
NmeAIII	GCCGAG	1 cut(s)	1275
NmuCI	GTSAC	4 cut(s)	163, 169, 491, 1223
NsbI	TGCGCA	1 cut(s)	842
NsiI	ATGCAT	3 cut(s)	979, 1089, 1346
NspI	RCATGY	5 cut(s)	736, 1087, 1091, 1231, 1344
OliI	CACNNNNGTG	1 cut(s)	1065
PaeI	GCATGC	1 cut(s)	1091
PceI	AGGCCT	1 cut(s)	1429
PciI	ACATGT	2 cut(s)	732, 1227
PciSI	GCTCTTC	1 cut(s)	1338
PctI	GAATGC	3 cut(s)	202, 231, 567
PdmI	GAANNNNTTC	3 cut(s)	456, 686, 900
PfeI	GAWTC	4 cut(s)	515, 617, 1186, 1505
PflMI	CCANNNNNTGG	1 cut(s)	1043
PinAI	ACCGGT	1 cut(s)	1100
PkrI	GCNGC	6 cut(s)	85, 263, 364, 1443, 1455, 1519
PleI	GAGTC	1 cut(s)	274
PpsI	GAGTC	1 cut(s)	274
PpuMI	RGGWCCY	1 cut(s)	937
PscI	ACATGT	2 cut(s)	732, 1227
PsiI	TTATAA	1 cut(s)	206
Psp5II	RGGWCCY	1 cut(s)	937
Psp6I	CCWGG	4 cut(s)	256, 442, 1070, 1372
PspFI	CCCAGC	1 cut(s)	45
PspGI	CCWGG	4 cut(s)	256, 442, 1070, 1372
PspN4I	GGNNCC	4 cut(s)	664, 1035, 1049, 1069
PspPI	GGNCC	2 cut(s)	937, 1034
PspPPI	RGGWCCY	1 cut(s)	937
PstI	CTGCAG	2 cut(s)	313, 613
PstNI	CAGNNNCTG	2 cut(s)	185, 797
PsuI	RGATCY	1 cut(s)	181
RsaI	GTAC	3 cut(s)	750, 759, 802
RsaNI	GTAC	3 cut(s)	749, 758, 801
RseI	CAYNNNNRTG	2 cut(s)	1065, 1359
SapI	GCTCTTC	1 cut(s)	1338
SaqAI	TTAA	8 cut(s)	156, 267, 422, 669, 834, 1007, 1143, 1314
SatI	GCNGC	6 cut(s)	84, 262, 363, 1442, 1454, 1518
Sau3AI	GATC	9 cut(s)	142, 181, 637, 642, 1114, 1159, 1195, 1479, 1543
Sau96I	GGNCC	2 cut(s)	937, 1034
ScaI	AGTACT	1 cut(s)	759
SchI	GAGTC	1 cut(s)	274
ScrFI	CCNGG	4 cut(s)	258, 444, 1072, 1374
SduI	GDGCHC	1 cut(s)	732
SfaNI	GCATC	6 cut(s)	253, 350, 816, 986, 1331, 1390
SfcI	CTRYAG	2 cut(s)	309, 609
SinI	GGWCC	2 cut(s)	937, 1034
SmiMI	CAYNNNNRTG	2 cut(s)	1065, 1359
SmlI	CTYRAG	1 cut(s)	146
SmoI	CTYRAG	1 cut(s)	146
SphI	GCATGC	1 cut(s)	1091
Sse9I	AATT	8 cut(s)	11, 353, 384, 510, 685, 812, 1202, 1470
SseBI	AGGCCT	1 cut(s)	1429
SsiI	CCGC	6 cut(s)	262, 330, 829, 1165, 1414, 1517
SspMI	CTAG	2 cut(s)	981, 1124
StuI	AGGCCT	1 cut(s)	1429
StyD4I	CCNGG	4 cut(s)	256, 442, 1070, 1372
StyI	CCWWGG	4 cut(s)	125, 552, 933, 1300
TaaI	ACNGT	4 cut(s)	169, 377, 998, 1176
TaiI	ACGT	1 cut(s)	165
TasI	AATT	8 cut(s)	11, 353, 384, 510, 685, 812, 1202, 1470
TatI	WGTACW	1 cut(s)	757
TauI	GCSGC	2 cut(s)	264, 1520
TfiI	GAWTC	4 cut(s)	515, 617, 1186, 1505
Tru1I	TTAA	8 cut(s)	156, 267, 422, 669, 834, 1007, 1143, 1314
Tru9I	TTAA	8 cut(s)	156, 267, 422, 669, 834, 1007, 1143, 1314
TscAI	CASTG	4 cut(s)	172, 922, 1003, 1058
TseFI	GTSAC	4 cut(s)	163, 169, 491, 1223
TseI	GCWGC	4 cut(s)	83, 362, 1441, 1453
Tsp45I	GTSAC	4 cut(s)	163, 169, 491, 1223
TspDTI	ATGAA	5 cut(s)	531, 901, 1372, 1376, 1580
TspGWI	ACGGA	1 cut(s)	1269
TspRI	CASTG	4 cut(s)	172, 922, 1003, 1058
Van91I	CCANNNNNTGG	1 cut(s)	1043
VpaK11BI	GGWCC	2 cut(s)	937, 1034
XapI	RAATTY	2 cut(s)	685, 812
XbaI	TCTAGA	1 cut(s)	980
XceI	RCATGY	5 cut(s)	736, 1087, 1091, 1231, 1344
XmiI	GTMKAC	1 cut(s)	1076
XmnI	GAANNNNTTC	3 cut(s)	456, 686, 900
XspI	CTAG	2 cut(s)	981, 1124
ZraI	GACGTC	1 cut(s)	163
ZrmI	AGTACT	1 cut(s)	759
Zsp2I	ATGCAT	3 cut(s)	979, 1089, 1346

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.