Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0002969

Physical Location & Seq

Genomic Coordinates Reverse (-)

8291393 .. 8297384

Gene Structure 5992 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g09370.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGGCTGGTGAAGTAGTGACTGCAAATGGAAATGGAAACGGCAACGGCAACACCCAGCTAAATCCACAGAGGACTTACCAAGTTGTTGTGGCTGCAACACGACAAATGGGTATTGGAAAGGATGGGAAACTACCTTGGAGACTGCCTTCTGATCTCAAGTTCTTTAAGGAGGTCACTGTGACCACATCAGATCCTGGAAAAAAGAATGCAATTATAATGGGTAGAAAAACATGGGAGAGCATTCCCCCTGAGCATCGGCCTCTACCTGGACGCCTTAATGTTGTCCTGACTCGTTCTGGGAGTTTTGATATTGCTACTGCTGAGAATGTTGTCATATGTGGGAGCATCGCCTCTGCTCTGGAGCTGTTAGCTGCTTCTCCTTACTGTCTGTCAATTGACAAAGTGTTTCTCATAGGAGGTGGCCAGATATTGAGGGAAGCTCTCAATGGGCCTGGATGTGATGCAATCCACATTACAGAAATAGAGACCAACATTGAATGCGACACTTGTATCCCTGCAATTGATTCCGCAGTTTTTCAGCCATGGTATTCATCATTTCCCAAGGTGGAGAATAGCATTCGTCATTCCTTCACAACTTATGTACGTGTGAGGAGTTCTGAAGTTGGATCCCTTTGTGAAAACAATGATCTGAACTGTAATAGTAGGTCAGATTCCATTAAAGAGGTAAAGAAGTTCCCTTTCCTCCCCAAGATGATCTTTGAGAAACATGAGGAGTACATGTATCTAAATCTGGTTCAAGAAATAATCTCAGATGGTACTTCTAAGGATGATAGGACTGGGACTGGTACTTTATCAAAATTTGGTTGCCAGATGCGGTTCAATCTGCGCAGAACTTTTCCTCTTCTAACAACCAAGAAAGTATTTTGGCGAGGGGTTGTTGAAGAACTTCTGTGGTTCCTCAGTGGTTCAACCAATGCAAAGGTTCTTCAGGAGAAAGGCATTCACATATGGGATGGAAATGCATCTAGAGACTACCTTGATAGCATTGGTTTAACTGGCAGGGAGGAGGGCGACTTGGGACCGGTATATGGGTTCCAGTGGCGACATTTTGGTGCCAGGTACACTGACATGCATGCTGACTACACTGGCCAAGGATTTGACCAGTTATTAGATGTTATTGACAAGATAAAAAATAATCCTGATGATCGACGGATTATACTTTCAGCTTGGAATCCTTCTGATCTCAAATTGATGGCCCTCCCACCTTGTCACATGTTTGCTCAGTTCTATGTTGCCAAAGGAGAGTTATCATGCCAAATGTACCAGCGTTCTGCTGACATGGGCCTGGGCGTGCCATTTAACATTGCCTCATATGCGCTTCTGACATGCATGATTGCTCATGTTTGTGATCTTGTTCCAGGTGATTTCGTTCATGTTTTAGGGGATGCTCATGTTTACCGCACTCATATCAGGCCTCTAGAGGAGCAACTTAAGAAACTGCCCAAGCCTTTTCCAATTTTGAAGATTAATCCTGAGAAAAAGAATATAGATTCTTTTGTGGCTGCTGATTTCGAACTCAGTGGTTATGATCCTCATGAGAAGATAGAAATGAAAATGGCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004146 GO:0004799 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0009058 GO:0009108 GO:0009256 GO:0009257 GO:0009396 GO:0009987 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016740 GO:0016741 GO:0018130 GO:0019438 GO:0019752 GO:0032259 GO:0034641 GO:0042083 GO:0042398 GO:0042558 GO:0042559 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko00670 ko00790 ko01100 map00240 map00670 map00790 map01100

Pfam Domains

Protein Families

DHFR_1 Thymidylat_synt

Protein Analysis

529

Amino Acids

59.03

Weight (kDa)

6.38

Isoelectric Point (pI)

43.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHFR_1	PF00186	27 - 202	1.2e-36	Dihydrofolate reductase
Thymidylat_synt	PF00303	247 - 513	3.7e-100	Thymidylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16370 AT2G16370 AT2G16370 AT4G34570 AT4G34570 AT4G34570 AT4G34570
fragaria_vesca	FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370
malus_domestica	MD05G1063200.v1.1 MD10G1070700.v1.1
prunus_persica	Prupe.8G092500_v2.0.a1 Prupe.8G092500_v2.0.a1
pyrus_communis	pycom05g05460 pycom05g05470 pycom10g05650
rosa_chinensis	RchiOBHm_Chr6g0265641
rosa_laevigata	RLG00000014170
rosa_multiflora	Rmu_sc0003233.1_g000014 Rmu_sc0003233.1_g000015 Rmu_sc0009907.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432460 Rroxscaffold_6G00388530 Rroxscaffold_7G00202550
rosa_rugosa	Rorug06G0018100
rosa_samantha	Rh1BG086900 Rh3BG370300 Rh3CG367200 Rh6AG140100 Rh6BG140000 Rh6CG137100 Rh6DG123400
rosa_wichuraiana	Rw6G012050 Rw6G012100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	215
Acc16I	TGCGCA	1 cut(s)	848
AccB1I	GGYRCC	1 cut(s)	1073
AciI	CCGC	3 cut(s)	528, 835, 1420
AclWI	GGATC	4 cut(s)	185, 621, 634, 1544
AcoI	YGGCCR	2 cut(s)	421, 1108
AcsI	RAATTY	1 cut(s)	818
AcuI	CTGAAG	2 cut(s)	639, 932
AcyI	GRCGYC	1 cut(s)	271
AfaI	GTAC	6 cut(s)	603, 737, 778, 808, 1082, 1283
AfiI	CCNNNNNNNGG	2 cut(s)	266, 1049
AflII	CTTAAG	1 cut(s)	1451
AflIII	ACRYGT	3 cut(s)	604, 738, 1233
AgeI	ACCGGT	1 cut(s)	1042
AgsI	TTSAA	6 cut(s)	497, 758, 841, 902, 930, 1483
AjnI	CCWGG	6 cut(s)	193, 265, 451, 1076, 1305, 1378
AluBI	AGCT	5 cut(s)	58, 364, 371, 440, 1187
AluI	AGCT	5 cut(s)	58, 364, 371, 440, 1187
Alw26I	GTCTC	3 cut(s)	133, 479, 984
AlwI	GGATC	4 cut(s)	185, 621, 634, 1544
AlwNI	CAGNNNCTG	1 cut(s)	194
AoxI	GGCC	7 cut(s)	257, 421, 449, 1108, 1215, 1303, 1433
ApeKI	GCWGC	3 cut(s)	92, 371, 1523
ApoI	RAATTY	1 cut(s)	818
AseI	ATTAAT	1 cut(s)	1488
AsiGI	ACCGGT	1 cut(s)	1042
Asp700I	GAANNNNTTC	1 cut(s)	906
AspLEI	GCGC	2 cut(s)	849, 1339
AspS9I	GGNCC	4 cut(s)	449, 1040, 1216, 1303
AsuHPI	GGTGA	2 cut(s)	20, 1394
AsuII	TTCGAA	1 cut(s)	1533
AvaII	GGWCC	1 cut(s)	1040
BaeI	ACNNNNGTAYC	2 cut(s)	493, 526
BalI	TGGCCA	2 cut(s)	423, 1110
BamHI	GGATCC	1 cut(s)	626
BanI	GGYRCC	1 cut(s)	1073
BbvI	GCAGC	3 cut(s)	79, 358, 1510
BccI	CCATC	4 cut(s)	116, 767, 968, 1207
BceAI	ACGGC	2 cut(s)	55, 61
BciT130I	CCWGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
BciVI	GTATCC	1 cut(s)	521
BcoDI	GTCTC	3 cut(s)	133, 479, 984
BfaI	CTAG	2 cut(s)	987, 1439
BfrI	CTTAAG	1 cut(s)	1451
BfuI	GTATCC	1 cut(s)	521
BisI	GCNGC	3 cut(s)	93, 372, 1524
BlsI	GCNGC	3 cut(s)	94, 373, 1525
Bme1390I	CCNGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
Bme18I	GGWCC	1 cut(s)	1040
BmgT120I	GGNCC	4 cut(s)	449, 1040, 1216, 1303
BmiI	GGNNCC	5 cut(s)	628, 917, 1041, 1055, 1075
BmrFI	CCNGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
BmrI	ACTGGG	1 cut(s)	807
BmsI	GCATC	6 cut(s)	262, 354, 451, 822, 992, 1396
BmuI	ACTGGG	1 cut(s)	807
BplI	GAGNNNNNCTC	2 cut(s)	424, 456
BpmI	CTGGAG	1 cut(s)	380
Bpu10I	CCTNAGC	1 cut(s)	249
Bpu14I	TTCGAA	1 cut(s)	1533
BpuEI	CTTGAG	1 cut(s)	140
BsaAI	YACGTR	1 cut(s)	605
BsaHI	GRCGYC	1 cut(s)	271
BsaI	GGTCTC	1 cut(s)	479
BsaJI	CCNNGG	5 cut(s)	134, 542, 561, 1111, 1306
BsaWI	WCCGGW	1 cut(s)	1042
BsaXI	ACNNNNNCTCC	2 cut(s)	725, 755
Bsc4I	CCNNNNNNNGG	2 cut(s)	266, 1049
Bse118I	RCCGGY	1 cut(s)	1042
Bse1I	ACTGG	6 cut(s)	802, 808, 1021, 1057, 1111, 1123
Bse3DI	GCAATG	1 cut(s)	1323
BseBI	CCWGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
BseDI	CCNNGG	5 cut(s)	134, 542, 561, 1111, 1306
BseGI	GGATG	5 cut(s)	127, 461, 793, 979, 1411
BseLI	CCNNNNNNNGG	2 cut(s)	266, 1049
BseMI	GCAATG	1 cut(s)	1323
BseMII	CTCAG	7 cut(s)	240, 312, 783, 934, 1256, 1485, 1552
BseNI	ACTGG	6 cut(s)	802, 808, 1021, 1057, 1111, 1123
BseRI	GAGGAG	4 cut(s)	625, 746, 1040, 1457
BseXI	GCAGC	3 cut(s)	79, 358, 1510
BseYI	CCCAGC	1 cut(s)	54
BshFI	GGCC	7 cut(s)	259, 423, 451, 1110, 1217, 1305, 1435
BshNI	GGYRCC	1 cut(s)	1073
BshTI	ACCGGT	1 cut(s)	1042
BsiSI	CCGG	1 cut(s)	1043
BslFI	GGGAC	2 cut(s)	814, 1053
BslI	CCNNNNNNNGG	2 cut(s)	266, 1049
BsmAI	GTCTC	3 cut(s)	133, 479, 984
BsmFI	GGGAC	2 cut(s)	814, 1053
BsmI	GAATGC	5 cut(s)	211, 240, 503, 576, 960
BsnI	GGCC	7 cut(s)	259, 423, 451, 1110, 1217, 1305, 1435
Bso31I	GGTCTC	1 cut(s)	479
Bsp119I	TTCGAA	1 cut(s)	1533
Bsp143I	GATC	9 cut(s)	151, 190, 626, 646, 714, 1165, 1201, 1369, 1549
Bsp19I	CCATGG	1 cut(s)	542
BspACI	CCGC	3 cut(s)	528, 835, 1420
BspANI	GGCC	7 cut(s)	259, 423, 451, 1110, 1217, 1305, 1435
BspCNI	CTCAG	7 cut(s)	241, 313, 782, 933, 1255, 1486, 1551
BspHI	TCATGA	1 cut(s)	1555
BspLI	GGNNCC	5 cut(s)	628, 917, 1041, 1055, 1075
BspPI	GGATC	4 cut(s)	185, 621, 634, 1544
BspT104I	TTCGAA	1 cut(s)	1533
BspT107I	GGYRCC	1 cut(s)	1073
BspTI	CTTAAG	1 cut(s)	1451
BspTNI	GGTCTC	1 cut(s)	479
BsrDI	GCAATG	1 cut(s)	1323
BsrFI	RCCGGY	1 cut(s)	1042
BsrI	ACTGG	6 cut(s)	802, 808, 1021, 1057, 1111, 1123
BssAI	RCCGGY	1 cut(s)	1042
BssECI	CCNNGG	5 cut(s)	134, 542, 561, 1111, 1306
BssMI	GATC	9 cut(s)	151, 190, 626, 646, 714, 1165, 1201, 1369, 1549
BssNI	GRCGYC	1 cut(s)	271
BssT1I	CCWWGG	4 cut(s)	134, 542, 561, 1111
Bst2UI	CCWGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
Bst4CI	ACNGT	3 cut(s)	178, 386, 656
Bst6I	CTCTTC	1 cut(s)	867
BstACI	GRCGYC	1 cut(s)	271
BstAFI	CTTAAG	1 cut(s)	1451
BstBAI	YACGTR	1 cut(s)	605
BstBI	TTCGAA	1 cut(s)	1533
BstC8I	GCNNGC	2 cut(s)	1095, 1313
BstDEI	CTNAG	8 cut(s)	249, 321, 769, 783, 920, 1242, 1494, 1538
BstDSI	CCRYGG	1 cut(s)	542
BstF5I	GGATG	5 cut(s)	127, 461, 793, 979, 1411
BstHHI	GCGC	2 cut(s)	849, 1339
BstKTI	GATC	9 cut(s)	154, 193, 629, 649, 717, 1168, 1204, 1372, 1552
BstMAI	GTCTC	3 cut(s)	133, 479, 984
BstMBI	GATC	9 cut(s)	151, 190, 626, 646, 714, 1165, 1201, 1369, 1549
BstMWI	GCNNNNNNNGC	1 cut(s)	1334
BstNI	CCWGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
BstNSI	RCATGY	5 cut(s)	742, 1093, 1097, 1237, 1350
BstSCI	CCNGG	6 cut(s)	193, 265, 451, 1076, 1305, 1378
BstV1I	GCAGC	3 cut(s)	79, 358, 1510
BstX2I	RGATCY	2 cut(s)	190, 626
BstYI	RGATCY	2 cut(s)	190, 626
BsuI	GTATCC	1 cut(s)	521
BsuRI	GGCC	7 cut(s)	259, 423, 451, 1110, 1217, 1305, 1435
BtgI	CCRYGG	1 cut(s)	542
BtgZI	GCGATG	1 cut(s)	331
BtsCI	GGATG	5 cut(s)	127, 461, 793, 979, 1411
BtsIMutI	CAGTG	6 cut(s)	174, 928, 1064, 1083, 1104, 1546
Cac8I	GCNNGC	2 cut(s)	1095, 1313
CaiI	CAGNNNCTG	1 cut(s)	194
CciI	TCATGA	1 cut(s)	1555
CfoI	GCGC	2 cut(s)	849, 1339
Cfr10I	RCCGGY	1 cut(s)	1042
Cfr13I	GGNCC	4 cut(s)	449, 1040, 1216, 1303
CseI	GACGC	1 cut(s)	279
Csp6I	GTAC	6 cut(s)	602, 736, 777, 807, 1081, 1282
CspAI	ACCGGT	1 cut(s)	1042
CviQI	GTAC	6 cut(s)	602, 736, 777, 807, 1081, 1282
DdeI	CTNAG	8 cut(s)	249, 321, 769, 783, 920, 1242, 1494, 1538
DpnI	GATC	9 cut(s)	153, 192, 628, 648, 716, 1167, 1203, 1371, 1551
DpnII	GATC	9 cut(s)	151, 190, 626, 646, 714, 1165, 1201, 1369, 1549
EaeI	YGGCCR	2 cut(s)	421, 1108
Eam1104I	CTCTTC	1 cut(s)	867
EarI	CTCTTC	1 cut(s)	867
Eco130I	CCWWGG	4 cut(s)	134, 542, 561, 1111
Eco147I	AGGCCT	1 cut(s)	1435
Eco31I	GGTCTC	1 cut(s)	479
Eco47I	GGWCC	1 cut(s)	1040
Eco57I	CTGAAG	2 cut(s)	639, 932
EcoRII	CCWGG	6 cut(s)	193, 265, 451, 1076, 1305, 1378
EcoT14I	CCWWGG	4 cut(s)	134, 542, 561, 1111
EcoT22I	ATGCAT	3 cut(s)	985, 1095, 1352
ErhI	CCWWGG	4 cut(s)	134, 542, 561, 1111
FalI	AAGNNNNNCTT	2 cut(s)	701, 733
FaqI	GGGAC	2 cut(s)	814, 1053
FauNDI	CATATG	3 cut(s)	335, 968, 1333
Fnu4HI	GCNGC	3 cut(s)	93, 372, 1524
FokI	GGATG	5 cut(s)	134, 468, 800, 986, 1418
Fsp4HI	GCNGC	3 cut(s)	93, 372, 1524
FspBI	CTAG	2 cut(s)	987, 1439
FspI	TGCGCA	1 cut(s)	848
GlaI	GCGC	2 cut(s)	848, 1338
GluI	GCNGC	3 cut(s)	93, 372, 1524
GsaI	CCCAGC	1 cut(s)	58
GsuI	CTGGAG	1 cut(s)	380
HaeIII	GGCC	7 cut(s)	259, 423, 451, 1110, 1217, 1305, 1435
HapII	CCGG	1 cut(s)	1043
HgaI	GACGC	1 cut(s)	279
HhaI	GCGC	2 cut(s)	849, 1339
Hin1I	GRCGYC	1 cut(s)	271
Hin6I	GCGC	2 cut(s)	847, 1337
HinP1I	GCGC	2 cut(s)	847, 1337
HinfI	GANTC	5 cut(s)	289, 524, 671, 1192, 1511
HpaII	CCGG	1 cut(s)	1043
HphI	GGTGA	2 cut(s)	20, 1394
Hpy166II	GTNNAC	2 cut(s)	1083, 1417
Hpy188I	TCNGA	8 cut(s)	151, 190, 619, 651, 670, 772, 1201, 1344
Hpy188III	TCNNGA	9 cut(s)	286, 359, 758, 950, 987, 1160, 1439, 1493, 1556
Hpy8I	GTNNAC	2 cut(s)	1083, 1417
Hpy99I	CGWCG	1 cut(s)	1173
HpyAV	CCTTC	3 cut(s)	156, 598, 1206
HpyCH4III	ACNGT	3 cut(s)	178, 386, 656
HpyCH4IV	ACGT	1 cut(s)	604
HpyCH4V	TGCA	9 cut(s)	23, 95, 209, 464, 518, 938, 983, 1093, 1350
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1334
HpyF3I	CTNAG	8 cut(s)	249, 321, 769, 783, 920, 1242, 1494, 1538
HpySE526I	ACGT	1 cut(s)	604
Hsp92I	GRCGYC	1 cut(s)	271
HspAI	GCGC	2 cut(s)	847, 1337
Kzo9I	GATC	9 cut(s)	151, 190, 626, 646, 714, 1165, 1201, 1369, 1549
LmnI	GCTCC	3 cut(s)	342, 361, 1444
Lsp1109I	GCAGC	3 cut(s)	79, 358, 1510
LweI	GCATC	6 cut(s)	262, 354, 451, 822, 992, 1396
MaeI	CTAG	2 cut(s)	987, 1439
MaeII	ACGT	1 cut(s)	604
MaeIII	GTNAC	4 cut(s)	16, 172, 178, 1229
MalI	GATC	9 cut(s)	153, 192, 628, 648, 716, 1167, 1203, 1371, 1551
MboI	GATC	9 cut(s)	151, 190, 626, 646, 714, 1165, 1201, 1369, 1549
MboII	GAAGA	5 cut(s)	854, 914, 938, 1495, 1573
MfeI	CAATTG	2 cut(s)	393, 519
MflI	RGATCY	2 cut(s)	190, 626
MlsI	TGGCCA	2 cut(s)	423, 1110
MluCI	AATT	6 cut(s)	210, 393, 519, 818, 1208, 1476
MluNI	TGGCCA	2 cut(s)	423, 1110
MlyI	GAGTC	1 cut(s)	283
MmeI	TCCRAC	1 cut(s)	604
Mox20I	TGGCCA	2 cut(s)	423, 1110
Mph1103I	ATGCAT	3 cut(s)	985, 1095, 1352
MroXI	GAANNNNTTC	1 cut(s)	906
MscI	TGGCCA	2 cut(s)	423, 1110
MseI	TTAA	7 cut(s)	165, 276, 678, 1013, 1320, 1452, 1488
MslI	CAYNNNNRTG	3 cut(s)	1071, 1088, 1365
Msp20I	TGGCCA	2 cut(s)	423, 1110
MspCI	CTTAAG	1 cut(s)	1451
MspI	CCGG	1 cut(s)	1043
MspR9I	CCNGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
MunI	CAATTG	2 cut(s)	393, 519
Mva1269I	GAATGC	5 cut(s)	211, 240, 503, 576, 960
MvaI	CCWGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
MwoI	GCNNNNNNNGC	1 cut(s)	1334
NcoI	CCATGG	1 cut(s)	542
NdeI	CATATG	3 cut(s)	335, 968, 1333
NdeII	GATC	9 cut(s)	151, 190, 626, 646, 714, 1165, 1201, 1369, 1549
NlaIV	GGNNCC	5 cut(s)	628, 917, 1041, 1055, 1075
NmuCI	GTSAC	4 cut(s)	16, 172, 178, 1229
NsbI	TGCGCA	1 cut(s)	848
NsiI	ATGCAT	3 cut(s)	985, 1095, 1352
NspI	RCATGY	5 cut(s)	742, 1093, 1097, 1237, 1350
NspV	TTCGAA	1 cut(s)	1533
PaeI	GCATGC	1 cut(s)	1097
PagI	TCATGA	1 cut(s)	1555
PceI	AGGCCT	1 cut(s)	1435
PciI	ACATGT	2 cut(s)	738, 1233
PctI	GAATGC	5 cut(s)	211, 240, 503, 576, 960
PdmI	GAANNNNTTC	1 cut(s)	906
PfeI	GAWTC	4 cut(s)	524, 671, 1192, 1511
PfoI	TCCNGGA	1 cut(s)	193
PinAI	ACCGGT	1 cut(s)	1042
PkrI	GCNGC	3 cut(s)	94, 373, 1525
PleI	GAGTC	1 cut(s)	283
PpsI	GAGTC	1 cut(s)	283
Ppu21I	YACGTR	1 cut(s)	605
PscI	ACATGT	2 cut(s)	738, 1233
PshBI	ATTAAT	1 cut(s)	1488
PsiI	TTATAA	1 cut(s)	215
Psp6I	CCWGG	6 cut(s)	193, 265, 451, 1076, 1305, 1378
PspFI	CCCAGC	1 cut(s)	54
PspGI	CCWGG	6 cut(s)	193, 265, 451, 1076, 1305, 1378
PspN4I	GGNNCC	5 cut(s)	628, 917, 1041, 1055, 1075
PspPI	GGNCC	4 cut(s)	449, 1040, 1216, 1303
PstNI	CAGNNNCTG	1 cut(s)	194
PsuI	RGATCY	2 cut(s)	190, 626
RsaI	GTAC	6 cut(s)	603, 737, 778, 808, 1082, 1283
RsaNI	GTAC	6 cut(s)	602, 736, 777, 807, 1081, 1282
RseI	CAYNNNNRTG	3 cut(s)	1071, 1088, 1365
SaqAI	TTAA	7 cut(s)	165, 276, 678, 1013, 1320, 1452, 1488
SatI	GCNGC	3 cut(s)	93, 372, 1524
Sau3AI	GATC	9 cut(s)	151, 190, 626, 646, 714, 1165, 1201, 1369, 1549
Sau96I	GGNCC	4 cut(s)	449, 1040, 1216, 1303
SchI	GAGTC	1 cut(s)	283
ScrFI	CCNGG	6 cut(s)	195, 267, 453, 1078, 1307, 1380
SfaNI	GCATC	6 cut(s)	262, 354, 451, 822, 992, 1396
SfuI	TTCGAA	1 cut(s)	1533
SinI	GGWCC	1 cut(s)	1040
SmiMI	CAYNNNNRTG	3 cut(s)	1071, 1088, 1365
SmlI	CTYRAG	2 cut(s)	155, 1451
SmoI	CTYRAG	2 cut(s)	155, 1451
SphI	GCATGC	1 cut(s)	1097
Sse9I	AATT	6 cut(s)	210, 393, 519, 818, 1208, 1476
SseBI	AGGCCT	1 cut(s)	1435
SsiI	CCGC	3 cut(s)	528, 835, 1420
SspMI	CTAG	2 cut(s)	987, 1439
StuI	AGGCCT	1 cut(s)	1435
StyD4I	CCNGG	6 cut(s)	193, 265, 451, 1076, 1305, 1378
StyI	CCWWGG	4 cut(s)	134, 542, 561, 1111
TaaI	ACNGT	3 cut(s)	178, 386, 656
TaiI	ACGT	1 cut(s)	607
TaqI	TCGA	2 cut(s)	1168, 1533
TasI	AATT	6 cut(s)	210, 393, 519, 818, 1208, 1476
TatI	WGTACW	1 cut(s)	735
TfiI	GAWTC	4 cut(s)	524, 671, 1192, 1511
Tru1I	TTAA	7 cut(s)	165, 276, 678, 1013, 1320, 1452, 1488
Tru9I	TTAA	7 cut(s)	165, 276, 678, 1013, 1320, 1452, 1488
TscAI	CASTG	6 cut(s)	181, 928, 1064, 1090, 1111, 1546
TseFI	GTSAC	4 cut(s)	16, 172, 178, 1229
TseI	GCWGC	3 cut(s)	92, 371, 1523
Tsp45I	GTSAC	4 cut(s)	16, 172, 178, 1229
TspDTI	ATGAA	3 cut(s)	540, 1382, 1586
TspGWI	ACGGA	1 cut(s)	1186
TspRI	CASTG	6 cut(s)	181, 928, 1064, 1090, 1111, 1546
Vha464I	CTTAAG	1 cut(s)	1451
VpaK11BI	GGWCC	1 cut(s)	1040
VspI	ATTAAT	1 cut(s)	1488
XapI	RAATTY	1 cut(s)	818
XbaI	TCTAGA	2 cut(s)	986, 1438
XceI	RCATGY	5 cut(s)	742, 1093, 1097, 1237, 1350
XmnI	GAANNNNTTC	1 cut(s)	906
XspI	CTAG	2 cut(s)	987, 1439
Zsp2I	ATGCAT	3 cut(s)	985, 1095, 1352

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.