Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002969

Physical Location & Seq

Genomic Coordinates Forward (+)

11101757 .. 11106611

Gene Structure 4855 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1063200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGGTTCAACTATGGCTGGTGAATTGTTGGTGAGTGCAAATGGAAATGGTAACGCCCAACCCAATCCACAGAGGACGTACCAAGTTGTTGTGGCTGCGACTCGAGAAATGGGTATTGGAAAGGATGGGAAACTACCTTGGAGACTGCCGTCTGATCTCAAGTTCTTTAAGGACATCACTGTGACCACGTCAGATCCTGCGAAAAAGAATGCAATTATAATGGGTAGGAAAACATGGGAGAGCATTCTACCTGAGCATCAACCTCTACCTGGCCGCCTTAATGTTGTTCTGACTCGTTCCGGGAGTTTTGATATTGCTACTGCAGAGAATGTTGTGATATGCGGAAGCATGGCATCTGCTTTGGAATTGTTAGCTGCTTCTCCTTACTGTCTGTCAATTGAGAAAGTGTTTCTCATTGGAGGTGGCCAGATATTAAGGGAAGCTCTCAATGCGTCTGGCTGTGAAGCCATTCACATTACAGAAATAGAGACAAACATTGAATGTGACACTTTTATCCCTGCAATTGATTCCTCTGTATTTCAGCCGTGGTATTCATCCTTTCCTAAGGTTGAAAATGGCATTCGTCATTCTTTCACAACTTATGTTCGTGTAAGAAATTCTGCAGTTGAATCCCTTTGTGAAAACAATGATCTGAACTCTAATATTAAGTCAGACTCTGTGAAGTGTGAGGTGAAGAATTTTTCTTATCTGCCGAAGATGATTTTTGAGAAACATGAGGAGCACATGTATCTAAGGTTGGTTCGAGAAATACTCTCTGATGGCACTGCTAAGGATGACAGGACAGGGACTGGTACTTTGTCAAAATTCGGTTGCCAGAAAGTATTTTGGCGAGGGGTTGTTGAAGAACTTCTGTGGTTCATTAGTGGTTCAACAAATGCCAAGGTCCTTCAGGAGAAAGGCATTCACATATGGGATGGGAATGCATCTAGAGATTACCTTGACAGTGTTGGTTTAACTGACAAGGAAGAGGGCGACTTGGGACCAGTATATGGATTCCAGTGGCGACACTTTGGTGCCAGGTATACTGACATGCATGCTGACTACACGGGTCAAGGATTTGATCAGTTGCTAGATGTTACTGACAAGATTAAAAACAATCCGGATGATCGGCGGATTATACTCTCAGCATGGAATCCTTCTGATCTCAAATCGATGGCACTCCCACCTTGTCACATGTTTGCTCAGTTCTATGTAGCCAATGGAGAGTTATCATGTCAAATGTATCAGCGTTCTGCTGACATCGGCCTTGGCATTCCATTTAACATAGCATCATATGCTCTTCTGACATGCATGATTGCTCATGTTTGTGATCTTGTTCCTGGTGATTTCATTCATGTCTTAGGGGATGCTCATGTTTACCGCACTCACATCAGGCCTTTGCAGGAGCAGCTTGAAAAACTGCCCAAGCCTTTTCCAATTTTGAAGATCAATCCTGAGAAAAAGAATATAGATTCTTTTGCGGCTGCTGATTTCCAACTGATAGGTTATGATCCTCATCAGAAGATAGAAATGAAAATGGCCGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004146 GO:0004799 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0009058 GO:0009108 GO:0009256 GO:0009257 GO:0009396 GO:0009987 GO:0016053 GO:0016491 GO:0016645 GO:0016646 GO:0016740 GO:0016741 GO:0018130 GO:0019438 GO:0019752 GO:0032259 GO:0034641 GO:0042083 GO:0042398 GO:0042558 GO:0042559 GO:0043436 GO:0043603 GO:0043604 GO:0043648 GO:0043650 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko00670 ko00790 ko01100 map00240 map00670 map00790 map01100

Pfam Domains

Protein Families

DHFR_1 Thymidylat_synt

Protein Analysis

516

Amino Acids

57.44

Weight (kDa)

5.82

Isoelectric Point (pI)

42.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHFR_1	PF00186	28 - 203	4e-36	Dihydrofolate reductase
Thymidylat_synt	PF00303	249 - 515	5e-99	Thymidylate synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16370 AT2G16370 AT2G16370 AT4G34570 AT4G34570 AT4G34570 AT4G34570
fragaria_vesca	FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370 FvH4_2g09370
malus_domestica	MD05G1063200.v1.1 MD10G1070700.v1.1
prunus_persica	Prupe.8G092500_v2.0.a1 Prupe.8G092500_v2.0.a1
pyrus_communis	pycom05g05460 pycom05g05470 pycom10g05650
rosa_chinensis	RchiOBHm_Chr6g0265641
rosa_laevigata	RLG00000014170
rosa_multiflora	Rmu_sc0003233.1_g000014 Rmu_sc0003233.1_g000015 Rmu_sc0009907.1_g000015
rosa_roxburghii	Rroxscaffold_175G00432460 Rroxscaffold_6G00388530 Rroxscaffold_7G00202550
rosa_rugosa	Rorug06G0018100
rosa_samantha	Rh1BG086900 Rh3BG370300 Rh3CG367200 Rh6AG140100 Rh6BG140000 Rh6CG137100 Rh6DG123400
rosa_wichuraiana	Rw6G012050 Rw6G012100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	218
AccB1I	GGYRCC	1 cut(s)	1034
AccB7I	CCANNNNNTGG	1 cut(s)	1010
AccI	GTMKAC	1 cut(s)	1043
AccIII	TCCGGA	1 cut(s)	1120
AciI	CCGC	5 cut(s)	274, 342, 1132, 1381, 1481
AclWI	GGATC	2 cut(s)	188, 1505
AcoI	YGGCCR	3 cut(s)	271, 424, 1539
AcsI	RAATTY	3 cut(s)	616, 697, 824
AcuI	CTGAAG	1 cut(s)	893
AfaI	GTAC	2 cut(s)	80, 814
AfiI	CCNNNNNNNGG	2 cut(s)	269, 1010
AflIII	ACRYGT	2 cut(s)	744, 1194
AgsI	TTSAA	8 cut(s)	9, 500, 572, 629, 863, 891, 1415, 1444
AjiI	CACGTC	1 cut(s)	189
AjnI	CCWGG	3 cut(s)	268, 1037, 1339
AjuI	GAANNNNNNNTTGG	2 cut(s)	99, 131
AleI	CACNNNNGTG	1 cut(s)	1032
AluBI	AGCT	3 cut(s)	374, 443, 1411
AluI	AGCT	3 cut(s)	374, 443, 1411
Alw21I	GWGCWC	1 cut(s)	744
Alw26I	GTCTC	2 cut(s)	136, 482
AlwI	GGATC	2 cut(s)	188, 1505
AlwNI	CAGNNNCTG	3 cut(s)	197, 677, 809
Ama87I	CYCGRG	1 cut(s)	102
Aor13HI	TCCGGA	1 cut(s)	1120
AoxI	GGCC	5 cut(s)	271, 424, 1264, 1394, 1539
ApeKI	GCWGC	4 cut(s)	95, 374, 1408, 1484
ApoI	RAATTY	3 cut(s)	616, 697, 824
ArsI	GACNNNNNNTTYG	2 cut(s)	799, 831
Asp700I	GAANNNNTTC	2 cut(s)	468, 867
AspS9I	GGNCC	2 cut(s)	904, 1001
AsuC2I	CCSGG	1 cut(s)	301
AsuHPI	GGTGA	4 cut(s)	32, 43, 703, 1355
AvaI	CYCGRG	1 cut(s)	102
AvaII	GGWCC	2 cut(s)	904, 1001
AxyI	CCTNAGG	1 cut(s)	564
BalI	TGGCCA	1 cut(s)	426
BanI	GGYRCC	1 cut(s)	1034
Bbv12I	GWGCWC	1 cut(s)	744
BbvI	GCAGC	4 cut(s)	82, 361, 1420, 1471
BccI	CCATC	4 cut(s)	119, 773, 929, 1168
BceAI	ACGGC	3 cut(s)	133, 529, 1526
BciT130I	CCWGG	3 cut(s)	270, 1039, 1341
BclI	TGATCA	1 cut(s)	1081
BcnI	CCSGG	1 cut(s)	301
BcoDI	GTCTC	2 cut(s)	136, 482
BfaI	CTAG	2 cut(s)	948, 1091
BfmI	CTRYAG	2 cut(s)	321, 621
BisI	GCNGC	6 cut(s)	96, 274, 375, 1409, 1482, 1485
BlsI	GCNGC	6 cut(s)	97, 275, 376, 1410, 1483, 1486
Bme1390I	CCNGG	4 cut(s)	270, 301, 1039, 1341
Bme18I	GGWCC	2 cut(s)	904, 1001
BmeT110I	CYCGRG	1 cut(s)	102
BmgBI	CACGTC	1 cut(s)	189
BmgT120I	GGNCC	2 cut(s)	904, 1001
BmiI	GGNNCC	2 cut(s)	1002, 1036
BmrFI	CCNGG	4 cut(s)	270, 301, 1039, 1341
BmsI	GCATC	5 cut(s)	265, 362, 953, 1298, 1357
BoxI	GACNNNNGTC	1 cut(s)	148
BplI	GAGNNNNNCTC	2 cut(s)	756, 788
Bpu10I	CCTNAGC	2 cut(s)	252, 789
BpuEI	CTTGAG	1 cut(s)	143
BpuMI	CCSGG	1 cut(s)	301
Bsa29I	ATCGAT	1 cut(s)	1172
BsaBI	GATNNNNATC	1 cut(s)	1515
BsaJI	CCNNGG	4 cut(s)	137, 545, 900, 1267
BsaWI	WCCGGW	1 cut(s)	1120
BsaXI	ACNNNNNCTCC	2 cut(s)	731, 761
Bsc4I	CCNNNNNNNGG	2 cut(s)	269, 1010
Bse1I	ACTGG	3 cut(s)	814, 1004, 1018
Bse21I	CCTNAGG	1 cut(s)	564
Bse8I	GATNNNNATC	1 cut(s)	1515
BseAI	TCCGGA	1 cut(s)	1120
BseBI	CCWGG	3 cut(s)	270, 1039, 1341
BseCI	ATCGAT	1 cut(s)	1172
BseDI	CCNNGG	4 cut(s)	137, 545, 900, 1267
BseGI	GGATG	6 cut(s)	130, 554, 799, 940, 1129, 1372
BseJI	GATNNNNATC	1 cut(s)	1515
BseLI	CCNNNNNNNGG	2 cut(s)	269, 1010
BseMII	CTCAG	4 cut(s)	243, 1158, 1217, 1446
BseNI	ACTGG	3 cut(s)	814, 1004, 1018
BseRI	GAGGAG	1 cut(s)	752
BseXI	GCAGC	4 cut(s)	82, 361, 1420, 1471
BshFI	GGCC	5 cut(s)	273, 426, 1266, 1396, 1541
BshNI	GGYRCC	1 cut(s)	1034
BshVI	ATCGAT	1 cut(s)	1172
BsiHKAI	GWGCWC	1 cut(s)	744
BsiHKCI	CYCGRG	1 cut(s)	102
BsiSI	CCGG	2 cut(s)	300, 1121
BslFI	GGGAC	2 cut(s)	820, 1014
BslI	CCNNNNNNNGG	2 cut(s)	269, 1010
BsmAI	GTCTC	2 cut(s)	136, 482
BsmFI	GGGAC	2 cut(s)	820, 1014
BsmI	GAATGC	6 cut(s)	214, 243, 579, 921, 946, 1272
BsnI	GGCC	5 cut(s)	273, 426, 1266, 1396, 1541
BsoBI	CYCGRG	1 cut(s)	102
Bsp1286I	GDGCHC	1 cut(s)	744
Bsp13I	TCCGGA	1 cut(s)	1120
Bsp143I	GATC	9 cut(s)	154, 193, 649, 1081, 1126, 1162, 1330, 1446, 1510
BspACI	CCGC	5 cut(s)	274, 342, 1132, 1381, 1481
BspANI	GGCC	5 cut(s)	273, 426, 1266, 1396, 1541
BspCNI	CTCAG	4 cut(s)	244, 1157, 1216, 1447
BspDI	ATCGAT	1 cut(s)	1172
BspEI	TCCGGA	1 cut(s)	1120
BspLI	GGNNCC	2 cut(s)	1002, 1036
BspMAI	CTGCAG	2 cut(s)	325, 625
BspPI	GGATC	2 cut(s)	188, 1505
BspQI	GCTCTTC	1 cut(s)	1305
BspT107I	GGYRCC	1 cut(s)	1034
BsrI	ACTGG	3 cut(s)	814, 1004, 1018
BssECI	CCNNGG	4 cut(s)	137, 545, 900, 1267
BssMI	GATC	9 cut(s)	154, 193, 649, 1081, 1126, 1162, 1330, 1446, 1510
BssNAI	GTATAC	1 cut(s)	1044
BssT1I	CCWWGG	3 cut(s)	137, 900, 1267
Bst1107I	GTATAC	1 cut(s)	1044
Bst2UI	CCWGG	3 cut(s)	270, 1039, 1341
Bst4CI	ACNGT	3 cut(s)	181, 389, 965
Bst6I	CTCTTC	2 cut(s)	981, 1305
BstAPI	GCANNNNNTGC	1 cut(s)	1295
BstC8I	GCNNGC	1 cut(s)	1056
BstDEI	CTNAG	8 cut(s)	252, 564, 752, 789, 1144, 1203, 1360, 1455
BstDSI	CCRYGG	1 cut(s)	545
BstF5I	GGATG	6 cut(s)	130, 554, 799, 940, 1129, 1372
BstKTI	GATC	9 cut(s)	157, 196, 652, 1084, 1129, 1165, 1333, 1449, 1513
BstMAI	GTCTC	2 cut(s)	136, 482
BstMBI	GATC	9 cut(s)	154, 193, 649, 1081, 1126, 1162, 1330, 1446, 1510
BstMWI	GCNNNNNNNGC	3 cut(s)	449, 1295, 1408
BstNI	CCWGG	3 cut(s)	270, 1039, 1341
BstNSI	RCATGY	5 cut(s)	748, 1054, 1058, 1198, 1311
BstPAI	GACNNNNGTC	1 cut(s)	148
BstSCI	CCNGG	4 cut(s)	268, 299, 1037, 1339
BstSFI	CTRYAG	2 cut(s)	321, 621
BstV1I	GCAGC	4 cut(s)	82, 361, 1420, 1471
BstX2I	RGATCY	1 cut(s)	193
BstYI	RGATCY	1 cut(s)	193
BstZ17I	GTATAC	1 cut(s)	1044
Bsu15I	ATCGAT	1 cut(s)	1172
Bsu36I	CCTNAGG	1 cut(s)	564
BsuRI	GGCC	5 cut(s)	273, 426, 1266, 1396, 1541
BsuTUI	ATCGAT	1 cut(s)	1172
BtgI	CCRYGG	1 cut(s)	545
BtrI	CACGTC	1 cut(s)	189
BtsCI	GGATG	6 cut(s)	130, 554, 799, 940, 1129, 1372
BtsI	GCAGTG	1 cut(s)	783
BtsIMutI	CAGTG	4 cut(s)	177, 783, 970, 1025
Cac8I	GCNNGC	1 cut(s)	1056
CaiI	CAGNNNCTG	3 cut(s)	197, 677, 809
Cfr13I	GGNCC	2 cut(s)	904, 1001
ClaI	ATCGAT	1 cut(s)	1172
CseI	GACGC	1 cut(s)	441
Csp6I	GTAC	2 cut(s)	79, 813
CviQI	GTAC	2 cut(s)	79, 813
DdeI	CTNAG	8 cut(s)	252, 564, 752, 789, 1144, 1203, 1360, 1455
DpnI	GATC	9 cut(s)	156, 195, 651, 1083, 1128, 1164, 1332, 1448, 1512
DpnII	GATC	9 cut(s)	154, 193, 649, 1081, 1126, 1162, 1330, 1446, 1510
EaeI	YGGCCR	3 cut(s)	271, 424, 1539
Eam1104I	CTCTTC	2 cut(s)	981, 1305
EarI	CTCTTC	2 cut(s)	981, 1305
EciI	GGCGGA	1 cut(s)	1147
Eco130I	CCWWGG	3 cut(s)	137, 900, 1267
Eco147I	AGGCCT	1 cut(s)	1396
Eco47I	GGWCC	2 cut(s)	904, 1001
Eco57I	CTGAAG	1 cut(s)	893
Eco81I	CCTNAGG	1 cut(s)	564
Eco88I	CYCGRG	1 cut(s)	102
EcoO109I	RGGNCCY	1 cut(s)	904
EcoRII	CCWGG	3 cut(s)	268, 1037, 1339
EcoT14I	CCWWGG	3 cut(s)	137, 900, 1267
EcoT22I	ATGCAT	3 cut(s)	946, 1056, 1313
ErhI	CCWWGG	3 cut(s)	137, 900, 1267
FaqI	GGGAC	2 cut(s)	820, 1014
FauNDI	CATATG	2 cut(s)	929, 1294
FbaI	TGATCA	1 cut(s)	1081
FblI	GTMKAC	1 cut(s)	1043
Fnu4HI	GCNGC	6 cut(s)	96, 274, 375, 1409, 1482, 1485
FokI	GGATG	6 cut(s)	137, 541, 806, 947, 1136, 1379
Fsp4HI	GCNGC	6 cut(s)	96, 274, 375, 1409, 1482, 1485
FspBI	CTAG	2 cut(s)	948, 1091
GluI	GCNGC	6 cut(s)	96, 274, 375, 1409, 1482, 1485
HaeIII	GGCC	5 cut(s)	273, 426, 1266, 1396, 1541
HapII	CCGG	2 cut(s)	300, 1121
HgaI	GACGC	1 cut(s)	441
HinfI	GANTC	8 cut(s)	100, 292, 527, 629, 674, 1014, 1153, 1472
HpaII	CCGG	2 cut(s)	300, 1121
HphI	GGTGA	4 cut(s)	32, 43, 703, 1355
Hpy166II	GTNNAC	2 cut(s)	1044, 1378
Hpy188I	TCNGA	9 cut(s)	154, 193, 291, 654, 673, 778, 1162, 1305, 1521
Hpy188III	TCNNGA	6 cut(s)	104, 764, 911, 948, 1121, 1454
Hpy8I	GTNNAC	2 cut(s)	1044, 1378
HpyAV	CCTTC	2 cut(s)	917, 1167
HpyCH4III	ACNGT	3 cut(s)	181, 389, 965
HpyCH4IV	ACGT	2 cut(s)	77, 188
HpyCH4V	TGCA	9 cut(s)	38, 212, 323, 521, 623, 944, 1054, 1311, 1402
HpyF10VI	GCNNNNNNNGC	3 cut(s)	449, 1295, 1408
HpyF3I	CTNAG	8 cut(s)	252, 564, 752, 789, 1144, 1203, 1360, 1455
HpySE526I	ACGT	2 cut(s)	77, 188
Kpn2I	TCCGGA	1 cut(s)	1120
Ksp22I	TGATCA	1 cut(s)	1081
Kzo9I	GATC	9 cut(s)	154, 193, 649, 1081, 1126, 1162, 1330, 1446, 1510
LguI	GCTCTTC	1 cut(s)	1305
LmnI	GCTCC	2 cut(s)	739, 1405
Lsp1109I	GCAGC	4 cut(s)	82, 361, 1420, 1471
LweI	GCATC	5 cut(s)	265, 362, 953, 1298, 1357
MaeI	CTAG	2 cut(s)	948, 1091
MaeII	ACGT	2 cut(s)	77, 188
MaeIII	GTNAC	5 cut(s)	50, 181, 503, 1096, 1190
MalI	GATC	9 cut(s)	156, 195, 651, 1083, 1128, 1164, 1332, 1448, 1512
MboI	GATC	9 cut(s)	154, 193, 649, 1081, 1126, 1162, 1330, 1446, 1510
MboII	GAAGA	7 cut(s)	706, 727, 875, 998, 1292, 1456, 1534
MfeI	CAATTG	2 cut(s)	396, 522
MflI	RGATCY	1 cut(s)	193
MhlI	GDGCHC	1 cut(s)	744
MlsI	TGGCCA	1 cut(s)	426
MluCI	AATT	9 cut(s)	23, 213, 365, 396, 522, 616, 697, 824, 1437
MluNI	TGGCCA	1 cut(s)	426
MlyI	GAGTC	3 cut(s)	94, 286, 668
MmeI	TCCRAC	1 cut(s)	1519
MnlI	CCTC	9 cut(s)	66, 273, 413, 541, 682, 730, 845, 982, 1524
Mox20I	TGGCCA	1 cut(s)	426
Mph1103I	ATGCAT	3 cut(s)	946, 1056, 1313
MroI	TCCGGA	1 cut(s)	1120
MroXI	GAANNNNTTC	2 cut(s)	468, 867
MscI	TGGCCA	1 cut(s)	426
MseI	TTAA	7 cut(s)	168, 279, 434, 666, 974, 1110, 1281
MslI	CAYNNNNRTG	3 cut(s)	179, 1032, 1326
Msp20I	TGGCCA	1 cut(s)	426
MspI	CCGG	2 cut(s)	300, 1121
MspR9I	CCNGG	4 cut(s)	270, 301, 1039, 1341
MunI	CAATTG	2 cut(s)	396, 522
Mva1269I	GAATGC	6 cut(s)	214, 243, 579, 921, 946, 1272
MvaI	CCWGG	3 cut(s)	270, 1039, 1341
MwoI	GCNNNNNNNGC	3 cut(s)	449, 1295, 1408
NciI	CCSGG	1 cut(s)	301
NdeI	CATATG	2 cut(s)	929, 1294
NdeII	GATC	9 cut(s)	154, 193, 649, 1081, 1126, 1162, 1330, 1446, 1510
NlaIV	GGNNCC	2 cut(s)	1002, 1036
NmuCI	GTSAC	3 cut(s)	181, 503, 1190
NsiI	ATGCAT	3 cut(s)	946, 1056, 1313
NspI	RCATGY	5 cut(s)	748, 1054, 1058, 1198, 1311
OliI	CACNNNNGTG	1 cut(s)	1032
PaeI	GCATGC	1 cut(s)	1058
PaeR7I	CTCGAG	1 cut(s)	102
PceI	AGGCCT	1 cut(s)	1396
PciI	ACATGT	2 cut(s)	744, 1194
PciSI	GCTCTTC	1 cut(s)	1305
PctI	GAATGC	6 cut(s)	214, 243, 579, 921, 946, 1272
PdmI	GAANNNNTTC	2 cut(s)	468, 867
PfeI	GAWTC	5 cut(s)	527, 629, 1014, 1153, 1472
PflFI	GACNNNGTC	1 cut(s)	187
PflMI	CCANNNNNTGG	1 cut(s)	1010
PfoI	TCCNGGA	1 cut(s)	299
PkrI	GCNGC	6 cut(s)	97, 275, 376, 1410, 1483, 1486
PleI	GAGTC	3 cut(s)	94, 286, 668
PpsI	GAGTC	3 cut(s)	94, 286, 668
PpuMI	RGGWCCY	1 cut(s)	904
PscI	ACATGT	2 cut(s)	744, 1194
PshAI	GACNNNNGTC	1 cut(s)	148
PsiI	TTATAA	1 cut(s)	218
Psp5II	RGGWCCY	1 cut(s)	904
Psp6I	CCWGG	3 cut(s)	268, 1037, 1339
PspGI	CCWGG	3 cut(s)	268, 1037, 1339
PspN4I	GGNNCC	2 cut(s)	1002, 1036
PspPI	GGNCC	2 cut(s)	904, 1001
PspPPI	RGGWCCY	1 cut(s)	904
PstI	CTGCAG	2 cut(s)	325, 625
PstNI	CAGNNNCTG	3 cut(s)	197, 677, 809
PsuI	RGATCY	1 cut(s)	193
PsyI	GACNNNGTC	1 cut(s)	187
RsaI	GTAC	2 cut(s)	80, 814
RsaNI	GTAC	2 cut(s)	79, 813
RseI	CAYNNNNRTG	3 cut(s)	179, 1032, 1326
SapI	GCTCTTC	1 cut(s)	1305
SaqAI	TTAA	7 cut(s)	168, 279, 434, 666, 974, 1110, 1281
SatI	GCNGC	6 cut(s)	96, 274, 375, 1409, 1482, 1485
Sau3AI	GATC	9 cut(s)	154, 193, 649, 1081, 1126, 1162, 1330, 1446, 1510
Sau96I	GGNCC	2 cut(s)	904, 1001
SchI	GAGTC	3 cut(s)	94, 286, 668
ScrFI	CCNGG	4 cut(s)	270, 301, 1039, 1341
SduI	GDGCHC	1 cut(s)	744
SfaNI	GCATC	5 cut(s)	265, 362, 953, 1298, 1357
SfcI	CTRYAG	2 cut(s)	321, 621
Sfr274I	CTCGAG	1 cut(s)	102
SinI	GGWCC	2 cut(s)	904, 1001
SlaI	CTCGAG	1 cut(s)	102
SmiMI	CAYNNNNRTG	3 cut(s)	179, 1032, 1326
SmlI	CTYRAG	2 cut(s)	102, 158
SmoI	CTYRAG	2 cut(s)	102, 158
SphI	GCATGC	1 cut(s)	1058
Sse9I	AATT	9 cut(s)	23, 213, 365, 396, 522, 616, 697, 824, 1437
SseBI	AGGCCT	1 cut(s)	1396
SsiI	CCGC	5 cut(s)	274, 342, 1132, 1381, 1481
SspI	AATATT	1 cut(s)	664
SspMI	CTAG	2 cut(s)	948, 1091
StuI	AGGCCT	1 cut(s)	1396
StyD4I	CCNGG	4 cut(s)	268, 299, 1037, 1339
StyI	CCWWGG	3 cut(s)	137, 900, 1267
TaaI	ACNGT	3 cut(s)	181, 389, 965
TaiI	ACGT	2 cut(s)	80, 191
TaqI	TCGA	3 cut(s)	103, 763, 1172
TasI	AATT	9 cut(s)	23, 213, 365, 396, 522, 616, 697, 824, 1437
TauI	GCSGC	2 cut(s)	276, 1484
TfiI	GAWTC	5 cut(s)	527, 629, 1014, 1153, 1472
Tru1I	TTAA	7 cut(s)	168, 279, 434, 666, 974, 1110, 1281
Tru9I	TTAA	7 cut(s)	168, 279, 434, 666, 974, 1110, 1281
TscAI	CASTG	4 cut(s)	184, 790, 970, 1025
TseFI	GTSAC	3 cut(s)	181, 503, 1190
TseI	GCWGC	4 cut(s)	95, 374, 1408, 1484
Tsp45I	GTSAC	3 cut(s)	181, 503, 1190
TspDTI	ATGAA	5 cut(s)	543, 868, 1339, 1343, 1547
TspRI	CASTG	4 cut(s)	184, 790, 970, 1025
Tth111I	GACNNNGTC	1 cut(s)	187
Van91I	CCANNNNNTGG	1 cut(s)	1010
VpaK11BI	GGWCC	2 cut(s)	904, 1001
XapI	RAATTY	3 cut(s)	616, 697, 824
XbaI	TCTAGA	1 cut(s)	947
XceI	RCATGY	5 cut(s)	748, 1054, 1058, 1198, 1311
XhoI	CTCGAG	1 cut(s)	102
XmiI	GTMKAC	1 cut(s)	1043
XmnI	GAANNNNTTC	2 cut(s)	468, 867
XspI	CTAG	2 cut(s)	948, 1091
Zsp2I	ATGCAT	3 cut(s)	946, 1056, 1313

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.