AT2G17250

CBF/Mak21 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003349
Physical Location & Seq
Forward (+)
7499472 .. 7503195
3724 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G17250.1

Sequence Viewer

Length: 1734 bp
ATGGCGTCAATCCTCTCAAAGAAGCAGAAGAAGAACGAAAAATACACCTTGAAAGAGCTCAAATCATTAGGTCATGACTTACTCACTTCTCGATCTCACATCAATAACCTTCCTCTCCTTCTCACCTTCGTCTCACCAGAATCTCCGCCGCAGTTCGTCGTTGAATCACTCCTCTCACTCCAATCCTTTTTCACTCCTCTACTCTCTCAGCTTCCTCCGACTTCTTCGTCTCCTTCTTCAACCAAAACAGAGGACCCTGAAGTTGTGTTCAAAGCTTGGCTCAGATCAAAATTTGACGAATTCGTCAAGCTTTTACTCGATGTTCTTGTCTCTCAACAATCAGAAGATTCTCTAAGGGGAATTGTGTTGGGAACATTAATGGAATTTGTAAAGCTTTTGAATGCTGGAAGGTTTCATTCTTCAATCTACCACAGATTACTTGATGCTATTATTCACTCTGAGGTTGATATTGAGATATTCTTGGATATACTTACTTCAAAGTACTTCAAGTACATTGATGTCCGCTATTTTACATACATCAGCATGGAAAAGTTTGTGAAAACATTGGAAGCATCAGTGTCTGCTGATAGAACTGTGATCGAAAATAATGAGGCTGAGAGTGATTCAAAAGAAAGCCTAGAACTCTCTGTTCGCAAGATCTATCAAGTTTTATCTCAAATCCCACCTCCTGAGAAACAAGCTGAGAAGTCTCAACACGAGATGTGGAGTGGGTCAGATGAAAGTATTAGTGAGAAGCCGACAGATAAAAAGAAGAAAACTGAGAAAGGAGACAGCACTCTCCTTTCTCCGGCAACAATTAGCAAGAGGATGAAGTTGAAGTTCACTAAAGCATGGATTTCATTTCTCAGACTGCCTCTTCCCATTGATGTGTACAAGGAGGTTCTTGCCAGTATTCACCTGACAGTCATTCCCCATCTATCTAATCCTACAATGTTATGCGACTTTTTAACAAAATCGTACGACATTGGGGGTGTTGTCAGTGTGATGGCTCTTAGCAGTCTCTTCATCCTCATGACCCAACATGGTTTGGAGTATCCGTTCTTTTATGAAAAACTGTATGCGTTACTGGTTCCTTCTGTCTTTGTGGCAAAGCACCGAGCAAAATTTCTTCAGCTTCTTGACGCATGCCTGAAGTCGTCAATGCTTCCAGCATATCTGGCAGCTTCATTCACAAAGAAACTAAGCAGATTGTCACTTTCCATTCCCCCTGCGGGATCGTTGGTCATAACAGCTTTGATCTACAACCTTTTACGAAGAAATCCTACAATCAACCATCTTGTGCAGGAAATCGTTGAAAATGCCGATGAAGCCAACACAGAGGCTGGTGAACACAATGAGAGCCAACCAAAGACCATCAAAAAGCGAAAGCTTGGTATTGACTATTTCAACAACCAGGAGAGTGATCCCAAGAAATCTGGAGCCTTAAAAAGTTCTCTTTGGGAGATCGACACTTTGCGCCATCATTACTGTCCACCAGTTTCACGGTTTATCTCATCTCTAGAAACTAATCTAACCATCAGATCGAAAACCACGGAAATGAAAATTGAAGATTTTTGCTCTGGTTCATATGCCACCATATTTGGGGACGAGATTCGGAGAAGGGTAAAGCAAGTTCCTTTAGCGTTCTACAAAACGGTTCCAACATCTCTGTTTGCGGATTCAGATTTTCCTGGGTGGACGTTCACTATTCCACAAGAGGAAGGCACATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF RRP36

Protein Analysis

577

Amino Acids

65.35

Weight (kDa)

8.29

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 334 - 503 6e-45 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 226, 302
AciI CCGC 5 cut(s) 146, 149, 523, 1232, 1676
AclWI GGATC 2 cut(s) 1243, 1418
AcsI RAATTY 4 cut(s) 290, 299, 383, 1124
AcuI CTGAAG 3 cut(s) 279, 1115, 1172
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 503, 512, 893, 980
AfiI CCNNNNNNNGG 3 cut(s) 808, 1232, 1602
AjnI CCWGG 2 cut(s) 1413, 1690
AloI GAACNNNNNNTCC 2 cut(s) 1043, 1075
Alw21I GWGCWC 1 cut(s) 60
Alw26I GTCTC 6 cut(s) 136, 234, 334, 714, 783, 1025
AlwI GGATC 2 cut(s) 1243, 1418
AlwNI CAGNNNCTG 2 cut(s) 581, 1343
ApeKI GCWGC 1 cut(s) 1181
ApoI RAATTY 4 cut(s) 290, 299, 383, 1124
AseI ATTAAT 1 cut(s) 377
AspLEI GCGC 1 cut(s) 1479
AspS9I GGNCC 1 cut(s) 253
AsuHPI GGTGA 4 cut(s) 115, 126, 908, 1358
AvaII GGWCC 1 cut(s) 253
BanII GRGCYC 1 cut(s) 60
BarI GAAGNNNNNNTAC 2 cut(s) 1267, 1299
BauI CACGAG 1 cut(s) 716
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 1193
BccI CCATC 6 cut(s) 942, 1000, 1302, 1382, 1488, 1544
BciT130I CCWGG 2 cut(s) 1415, 1692
BciVI GTATCC 1 cut(s) 1065
BcoDI GTCTC 6 cut(s) 136, 234, 334, 714, 783, 1025
BfaI CTAG 2 cut(s) 638, 1520
BfuI GTATCC 1 cut(s) 1065
BglII AGATCT 1 cut(s) 657
BisI GCNGC 2 cut(s) 149, 1182
BlsI GCNGC 2 cut(s) 150, 1183
BmcAI AGTACT 1 cut(s) 503
Bme1390I CCNGG 2 cut(s) 1415, 1692
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 253
BmiI GGNNCC 4 cut(s) 255, 1092, 1441, 1659
BmrFI CCNGG 2 cut(s) 1415, 1692
BmsI GCATC 2 cut(s) 433, 581
BpmI CTGGAG 1 cut(s) 1458
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 1551, 1691
BsaXI ACNNNNNCTCC 2 cut(s) 1043, 1073
Bsc4I CCNNNNNNNGG 3 cut(s) 808, 1232, 1602
Bse1I ACTGG 3 cut(s) 909, 1092, 1496
BseBI CCWGG 2 cut(s) 1415, 1692
BseDI CCNNGG 2 cut(s) 1551, 1691
BseGI GGATG 2 cut(s) 834, 1026
BseLI CCNNNNNNNGG 3 cut(s) 808, 1232, 1602
BseMII CTCAG 8 cut(s) 221, 295, 450, 606, 681, 693, 771, 880
BseNI ACTGG 3 cut(s) 909, 1092, 1496
BseRI GAGGAG 2 cut(s) 161, 186
BseXI GCAGC 1 cut(s) 1193
BsgI GTGCAG 1 cut(s) 1322
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 1 cut(s) 809
BsiWI CGTACG 1 cut(s) 978
BslFI GGGAC 1 cut(s) 1619
BslI CCNNNNNNNGG 3 cut(s) 808, 1232, 1602
BsmAI GTCTC 6 cut(s) 136, 234, 334, 714, 783, 1025
BsmBI CGTCTC 2 cut(s) 136, 234
BsmFI GGGAC 1 cut(s) 1619
BsmI GAATGC 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 60
Bsp1407I TGTACA 1 cut(s) 891
Bsp143I GATC 9 cut(s) 92, 284, 597, 657, 1235, 1257, 1423, 1464, 1541
BspACI CCGC 5 cut(s) 146, 149, 523, 1232, 1676
BspCNI CTCAG 8 cut(s) 220, 294, 451, 607, 682, 694, 772, 879
BspHI TCATGA 2 cut(s) 73, 1032
BspLI GGNNCC 4 cut(s) 255, 1092, 1441, 1659
BspPI GGATC 2 cut(s) 1243, 1418
BsrGI TGTACA 1 cut(s) 891
BsrI ACTGG 3 cut(s) 909, 1092, 1496
BssECI CCNNGG 2 cut(s) 1551, 1691
BssMI GATC 9 cut(s) 92, 284, 597, 657, 1235, 1257, 1423, 1464, 1541
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 716
Bst2UI CCWGG 2 cut(s) 1415, 1692
Bst4CI ACNGT 6 cut(s) 595, 925, 1077, 1490, 1506, 1657
Bst6I CTCTTC 2 cut(s) 882, 1028
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 891
BstC8I GCNNGC 1 cut(s) 1147
BstDSI CCRYGG 1 cut(s) 1551
BstF5I GGATG 2 cut(s) 834, 1026
BstHHI GCGC 1 cut(s) 1479
BstKTI GATC 9 cut(s) 95, 287, 600, 660, 1238, 1260, 1426, 1467, 1544
BstMAI GTCTC 6 cut(s) 136, 234, 334, 714, 783, 1025
BstMBI GATC 9 cut(s) 92, 284, 597, 657, 1235, 1257, 1423, 1464, 1541
BstMWI GCNNNNNNNGC 2 cut(s) 1178, 1328
BstNI CCWGG 2 cut(s) 1415, 1692
BstNSI RCATGY 2 cut(s) 1149, 1731
BstSCI CCNGG 2 cut(s) 1413, 1690
BstV1I GCAGC 1 cut(s) 1193
BstX2I RGATCY 1 cut(s) 657
BstYI RGATCY 1 cut(s) 657
BsuI GTATCC 1 cut(s) 1065
BtgI CCRYGG 1 cut(s) 1551
BtsCI GGATG 2 cut(s) 834, 1026
BtsIMutI CAGTG 2 cut(s) 582, 1006
Cac8I GCNNGC 1 cut(s) 1147
CaiI CAGNNNCTG 2 cut(s) 581, 1343
CciI TCATGA 2 cut(s) 73, 1032
CfoI GCGC 1 cut(s) 1479
Cfr13I GGNCC 1 cut(s) 253
CseI GACGC 1 cut(s) 1151
Csp6I GTAC 4 cut(s) 502, 511, 892, 979
CspCI CAANNNNNGTGG 2 cut(s) 1582, 1617
CviAII CATG 7 cut(s) 74, 544, 852, 1033, 1043, 1146, 1728
CviQI GTAC 4 cut(s) 502, 511, 892, 979
DpnI GATC 9 cut(s) 94, 286, 599, 659, 1237, 1259, 1425, 1466, 1543
DpnII GATC 9 cut(s) 92, 284, 597, 657, 1235, 1257, 1423, 1464, 1541
DrdI GACNNNNNNGTC 2 cut(s) 226, 302
DseDI GACNNNNNNGTC 2 cut(s) 226, 302
Eam1104I CTCTTC 2 cut(s) 882, 1028
EarI CTCTTC 2 cut(s) 882, 1028
EciI GGCGGA 1 cut(s) 135
Ecl136II GAGCTC 1 cut(s) 58
Eco24I GRGCYC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 253
Eco53kI GAGCTC 1 cut(s) 58
Eco57I CTGAAG 3 cut(s) 279, 1115, 1172
EcoICRI GAGCTC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 253
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 1413, 1690
EcoT38I GRGCYC 1 cut(s) 60
Esp3I CGTCTC 2 cut(s) 136, 234
FaeI CATG 7 cut(s) 77, 547, 855, 1036, 1046, 1149, 1731
FaqI GGGAC 1 cut(s) 1619
FatI CATG 7 cut(s) 73, 543, 851, 1032, 1042, 1145, 1727
FauI CCCGC 1 cut(s) 1225
FauNDI CATATG 1 cut(s) 1588
Fnu4HI GCNGC 2 cut(s) 149, 1182
FokI GGATG 2 cut(s) 841, 1013
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 2 cut(s) 149, 1182
FspBI CTAG 2 cut(s) 638, 1520
GlaI GCGC 1 cut(s) 1478
GluI GCNGC 2 cut(s) 149, 1182
GsuI CTGGAG 1 cut(s) 1458
HapII CCGG 1 cut(s) 809
HgaI GACGC 1 cut(s) 1151
HhaI GCGC 1 cut(s) 1479
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 77, 547, 855, 1036, 1046, 1149, 1731
Hin6I GCGC 1 cut(s) 1477
HinP1I GCGC 1 cut(s) 1477
HindIII AAGCTT 4 cut(s) 273, 308, 392, 1388
HinfI GANTC 6 cut(s) 140, 164, 347, 623, 1612, 1679
HpaII CCGG 1 cut(s) 809
HphI GGTGA 4 cut(s) 115, 126, 908, 1358
Hpy166II GTNNAC 6 cut(s) 843, 892, 1349, 1493, 1698, 1704
Hpy188I TCNGA 9 cut(s) 219, 284, 343, 460, 736, 869, 1541, 1617, 1684
Hpy188III TCNNGA 7 cut(s) 74, 90, 689, 1033, 1139, 1437, 1520
Hpy8I GTNNAC 6 cut(s) 843, 892, 1349, 1493, 1698, 1704
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 8 cut(s) 119, 128, 136, 243, 402, 1104, 1614, 1715
HpyCH4III ACNGT 6 cut(s) 595, 925, 1077, 1490, 1506, 1657
HpyCH4IV ACGT 1 cut(s) 1700
HpyCH4V TGCA 1 cut(s) 1303
HpyF10VI GCNNNNNNNGC 2 cut(s) 1178, 1328
HpySE526I ACGT 1 cut(s) 1700
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 77, 547, 855, 1036, 1046, 1149, 1731
HspAI GCGC 1 cut(s) 1477
Kzo9I GATC 9 cut(s) 92, 284, 597, 657, 1235, 1257, 1423, 1464, 1541
LmnI GCTCC 1 cut(s) 1439
Lsp1109I GCAGC 1 cut(s) 1193
LweI GCATC 2 cut(s) 433, 581
MaeI CTAG 2 cut(s) 638, 1520
MaeII ACGT 1 cut(s) 1700
MaeIII GTNAC 2 cut(s) 1083, 1212
MalI GATC 9 cut(s) 94, 286, 599, 659, 1237, 1259, 1425, 1466, 1543
MboI GATC 9 cut(s) 92, 284, 597, 657, 1235, 1257, 1423, 1464, 1541
MflI RGATCY 1 cut(s) 657
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 7 cut(s) 290, 299, 360, 383, 816, 1124, 1563
MmeI TCCRAC 2 cut(s) 242, 1685
MseI TTAA 3 cut(s) 377, 968, 1445
MslI CAYNNNNRTG 4 cut(s) 542, 887, 1031, 1556
MspI CCGG 1 cut(s) 809
MspR9I CCNGG 2 cut(s) 1415, 1692
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 2 cut(s) 1415, 1692
MwoI GCNNNNNNNGC 2 cut(s) 1178, 1328
NdeI CATATG 1 cut(s) 1588
NdeII GATC 9 cut(s) 92, 284, 597, 657, 1235, 1257, 1423, 1464, 1541
NlaIII CATG 7 cut(s) 77, 547, 855, 1036, 1046, 1149, 1731
NlaIV GGNNCC 4 cut(s) 255, 1092, 1441, 1659
NmuCI GTSAC 1 cut(s) 1212
NspI RCATGY 2 cut(s) 1149, 1731
PaeI GCATGC 1 cut(s) 1149
PagI TCATGA 2 cut(s) 73, 1032
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 6 cut(s) 140, 164, 347, 623, 1612, 1679
Pfl23II CGTACG 1 cut(s) 978
PkrI GCNGC 2 cut(s) 150, 1183
PpuMI RGGWCCY 1 cut(s) 253
PshBI ATTAAT 1 cut(s) 377
Psp124BI GAGCTC 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 1413, 1690
PspGI CCWGG 2 cut(s) 1413, 1690
PspLI CGTACG 1 cut(s) 978
PspN4I GGNNCC 4 cut(s) 255, 1092, 1441, 1659
PspPI GGNCC 1 cut(s) 253
PspPPI RGGWCCY 1 cut(s) 253
PsrI GAACNNNNNNTAC 4 cut(s) 26, 58, 1617, 1649
PstNI CAGNNNCTG 2 cut(s) 581, 1343
PsuI RGATCY 1 cut(s) 657
RsaI GTAC 4 cut(s) 503, 512, 893, 980
RsaNI GTAC 4 cut(s) 502, 511, 892, 979
RseI CAYNNNNRTG 4 cut(s) 542, 887, 1031, 1556
SacI GAGCTC 1 cut(s) 60
SaqAI TTAA 3 cut(s) 377, 968, 1445
SatI GCNGC 2 cut(s) 149, 1182
Sau3AI GATC 9 cut(s) 92, 284, 597, 657, 1235, 1257, 1423, 1464, 1541
Sau96I GGNCC 1 cut(s) 253
ScaI AGTACT 1 cut(s) 503
ScrFI CCNGG 2 cut(s) 1415, 1692
SduI GDGCHC 1 cut(s) 60
SfaNI GCATC 2 cut(s) 433, 581
SinI GGWCC 1 cut(s) 253
SmiMI CAYNNNNRTG 4 cut(s) 542, 887, 1031, 1556
SphI GCATGC 1 cut(s) 1149
Sse9I AATT 7 cut(s) 290, 299, 360, 383, 816, 1124, 1563
SsiI CCGC 5 cut(s) 146, 149, 523, 1232, 1676
SspMI CTAG 2 cut(s) 638, 1520
SstI GAGCTC 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 1413, 1690
TaaI ACNGT 6 cut(s) 595, 925, 1077, 1490, 1506, 1657
TaiI ACGT 1 cut(s) 1703
TaqI TCGA 5 cut(s) 91, 318, 600, 1467, 1544
TasI AATT 7 cut(s) 290, 299, 360, 383, 816, 1124, 1563
TatI WGTACW 3 cut(s) 501, 510, 891
TauI GCSGC 1 cut(s) 151
TfiI GAWTC 6 cut(s) 140, 164, 347, 623, 1612, 1679
Tru1I TTAA 3 cut(s) 377, 968, 1445
Tru9I TTAA 3 cut(s) 377, 968, 1445
TscAI CASTG 2 cut(s) 582, 1006
TseFI GTSAC 1 cut(s) 1212
TseI GCWGC 1 cut(s) 1181
Tsp45I GTSAC 1 cut(s) 1212
TspGWI ACGGA 2 cut(s) 1047, 1568
TspRI CASTG 2 cut(s) 582, 1006
VpaK11BI GGWCC 1 cut(s) 253
VspI ATTAAT 1 cut(s) 377
XapI RAATTY 4 cut(s) 290, 299, 383, 1124
XbaI TCTAGA 1 cut(s) 1519
XceI RCATGY 2 cut(s) 1149, 1731
XspI CTAG 2 cut(s) 638, 1520
ZrmI AGTACT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.