MD05G1138800.v1.1

Nucleolar complex protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003349
Physical Location & Seq
Forward (+)
26919750 .. 26929724
9975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1138800.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGCCTCAATCCTCTCCAAGAAGCAGAAGCAGAAGCAAAAAGCCACCAAGCTTTCCGACCTCAAAACCCTAGGGAACGAGCTCCTCTCGTCTCGGGCCCACATCAACAACCTCCCCAAGCTCCTCTCCTTCGTTTCCCCGGCCTCGCCGCCTCAGTATATCGTCGAGTCCCTCCTCTCTCTCCAGTCATTCTTCACCCCTCTCCTCCCCGACCTCCCTCCCTCCTCCTCCAAGCCCTCCGCCTCCGATGGCTCCCACGACGACCCCGACTTCATATACCGAACCTGTCTCCGCTCCAAGTTCGACGAGCTCGTTGAGGTCCTCGTCGAAGTCCTGCTTTCTACGCAATCCGATGAAATTCTCAAGGTTTACACTACGTCACCAGTTGATTTGTTGTTAGACTTGCTTTCATCCAAGTATTTCAAGTATATTGATGTCCGTTATTTTACCTACATTAGCTTGGAAAAGCTTTCCAAAGTTCTGGATGCAAATGGGATTTCTGAGGATAAAAACATCAAGACAGACGGTAAAGATGTGGAGCATCCTAGGGAAAGATTTTTGTATAAAGGTGAATATGAAGACCTTTCTGGGAATCTGAAAGCAGATGGTAAACAGCACAAGACTGAGAAGCATAAGGATAACGTGTTGTCTGCAGCCAATATTGCCAAAAAGATGAAATTAAAATTTACTAAAGCATGGTTATCATTTCTAAGGTTACCACTTCCAGTTGATGTGTACAAGGAGGTTCTTGCCACACTCCATCAGGCAGTCATTCCTTTTCTAGCTAACCCTGTCTTGTTATGCGACTTCTTAACTAGATCGTATGACATTGGTGGTGCTATCAGCGTCATGGCTCTTAGCAGTCTTTTCATCCTCATGACGCAACATGGTTTAGAGTACCCCAACTTCTATGAGAAACTTTATGCATTACTGCTTGTAGATGCTTGCTTAAAATCGCCACTTCTTCCTGCATATTTGGCTGCGGCTTTTGCCAAGACATTGAGTAGGCTATCATTTTCAGTTCCTCTGTCTGGAGCGTTAGTTATTATTGCACTGGGAGGTGGCGGTGAAACTGGGAAAGATGATCCAGAAGCAGAACAGAGGGTTGCTGATGGCACGGATGGCACCGTAACTGGTTCGGCAGATAATTCAATAAAAAAATCAGGCATTGATCCTTTTGATAATGAACAAACTGATCCTCTGAAATCAAATGCCATGAGAAGTTCACTGGGGGAGATTGATACCGTCCGTCACCACTACTGCCCTGCTGTTTCAAGGTTCGTTTTGTCACTCGAGAATGATTTGACAGTTAGAGCCAAAACTACTGAGATATCCGTTGGAGATTTTAGCTCAGGTTCATATGCGACAATATTTGGAGAGCAGGTGAGACGAAGAATAAAGTCGGCTCCACTAGCATACTACAAAGCAGCTCCGACCTCTCTGTTCTCGGACTCGGAGTCTCAGTTTCTCGGTTGGACGTTCAAGTCTGAAGACAGTAAGCGAAAGAACGAAAGCGCTGATGAGAATGGGAAAATAGATAAAGAATGCGAAGATAGTCTGGTAAAACGACAGCGGATCAAGTGCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

530

Amino Acids

58.62

Weight (kDa)

6.78

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 282 - 428 1.1e-21 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1372
AasI GACNNNNNNGTC 1 cut(s) 1483
Acc36I ACCTGC 1 cut(s) 1372
AccB1I GGYRCC 1 cut(s) 1124
AccBSI CCGCTC 1 cut(s) 294
AciI CCGC 6 cut(s) 149, 240, 292, 983, 1065, 1573
AclWI GGATC 4 cut(s) 1079, 1166, 1190, 1583
AcsI RAATTY 2 cut(s) 357, 683
AcuI CTGAAG 1 cut(s) 1509
AfaI GTAC 2 cut(s) 737, 899
AfeI AGCGCT 1 cut(s) 1516
AfiI CCNNNNNNNGG 1 cut(s) 1031
AflIII ACRYGT 1 cut(s) 642
AgsI TTSAA 4 cut(s) 424, 1152, 1275, 1483
AhdI GACNNNNNGTC 1 cut(s) 1456
AluBI AGCT 9 cut(s) 52, 82, 121, 310, 459, 469, 785, 1350, 1430
AluI AGCT 9 cut(s) 52, 82, 121, 310, 459, 469, 785, 1350, 1430
Alw21I GWGCWC 2 cut(s) 84, 312
Alw26I GTCTC 4 cut(s) 96, 293, 1381, 1464
AlwI GGATC 4 cut(s) 1079, 1166, 1190, 1583
Ama87I CYCGRG 2 cut(s) 93, 1292
Aor51HI AGCGCT 1 cut(s) 1516
AoxI GGCC 3 cut(s) 3, 96, 141
ApaI GGGCCC 1 cut(s) 100
ApeKI GCWGC 3 cut(s) 653, 980, 1427
ApoI RAATTY 2 cut(s) 357, 683
AspA2I CCTAGG 2 cut(s) 70, 545
AspLEI GCGC 1 cut(s) 1517
AspS9I GGNCC 3 cut(s) 96, 97, 319
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 6 cut(s) 187, 372, 581, 1079, 1244, 1396
AvaI CYCGRG 2 cut(s) 93, 1292
AvaII GGWCC 1 cut(s) 319
AvrII CCTAGG 2 cut(s) 70, 545
BaeGI GKGCMC 1 cut(s) 100
BanI GGYRCC 1 cut(s) 1124
BanII GRGCYC 3 cut(s) 84, 100, 312
BarI GAAGNNNNNNTAC 2 cut(s) 890, 922
BbsI GAAGAC 2 cut(s) 585, 1497
Bbv12I GWGCWC 2 cut(s) 84, 312
BbvI GCAGC 3 cut(s) 665, 967, 1439
BccI CCATC 5 cut(s) 242, 599, 768, 1106, 1115
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 4 cut(s) 96, 293, 1381, 1464
BfaI CTAG 5 cut(s) 71, 546, 782, 816, 1412
BfmI CTRYAG 1 cut(s) 651
BfoI RGCGCY 1 cut(s) 1518
BfuAI ACCTGC 1 cut(s) 1372
BisI GCNGC 5 cut(s) 149, 654, 981, 984, 1428
BlnI CCTAGG 2 cut(s) 70, 545
BlsI GCNGC 5 cut(s) 150, 655, 982, 985, 1429
Bme1390I CCNGG 1 cut(s) 140
Bme18I GGWCC 1 cut(s) 319
BmeRI GACNNNNNGTC 1 cut(s) 1456
BmeT110I CYCGRG 2 cut(s) 93, 1292
BmgT120I GGNCC 3 cut(s) 96, 97, 319
BmiI GGNNCC 4 cut(s) 98, 253, 1126, 1407
BmrFI CCNGG 1 cut(s) 140
BmrI ACTGGG 3 cut(s) 1064, 1083, 1238
BmsI GCATC 3 cut(s) 475, 550, 931
BmuI ACTGGG 3 cut(s) 1064, 1083, 1238
BpiI GAAGAC 2 cut(s) 585, 1497
BplI GAGNNNNNCTC 2 cut(s) 71, 103
BpmI CTGGAG 2 cut(s) 167, 1053
Bpu10I CCTNAGC 1 cut(s) 1351
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 70, 138, 545
BsaXI ACNNNNNCTCC 6 cut(s) 1026, 1056, 1368, 1398, 1448, 1478
Bsc4I CCNNNNNNNGG 1 cut(s) 1031
Bse1I ACTGG 7 cut(s) 184, 383, 725, 1059, 1078, 1138, 1233
BseDI CCNNGG 3 cut(s) 70, 138, 545
BseGI GGATG 5 cut(s) 410, 490, 541, 870, 1126
BseLI CCNNNNNNNGG 1 cut(s) 1031
BseMII CTCAG 6 cut(s) 167, 492, 615, 1317, 1365, 1475
BseNI ACTGG 7 cut(s) 184, 383, 725, 1059, 1078, 1138, 1233
BseRI GAGGAG 6 cut(s) 74, 113, 164, 194, 214, 217
BseSI GKGCMC 1 cut(s) 100
BseXI GCAGC 3 cut(s) 665, 967, 1439
BshFI GGCC 3 cut(s) 5, 98, 143
BshNI GGYRCC 1 cut(s) 1124
BsiHKAI GWGCWC 2 cut(s) 84, 312
BsiHKCI CYCGRG 2 cut(s) 93, 1292
BsiSI CCGG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 1 cut(s) 1031
BsmAI GTCTC 4 cut(s) 96, 293, 1381, 1464
BsmBI CGTCTC 2 cut(s) 96, 1381
BsmFI GGGAC 1 cut(s) 154
BsmI GAATGC 1 cut(s) 1550
BsnI GGCC 3 cut(s) 5, 98, 143
BsoBI CYCGRG 2 cut(s) 93, 1292
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 3 cut(s) 84, 100, 312
Bsp1407I TGTACA 1 cut(s) 735
Bsp143I GATC 5 cut(s) 818, 1084, 1171, 1195, 1575
BspACI CCGC 6 cut(s) 149, 240, 292, 983, 1065, 1573
BspANI GGCC 3 cut(s) 5, 98, 143
BspCNI CTCAG 6 cut(s) 166, 493, 616, 1318, 1364, 1474
BspHI TCATGA 1 cut(s) 876
BspLI GGNNCC 4 cut(s) 98, 253, 1126, 1407
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 1372
BspPI GGATC 4 cut(s) 1079, 1166, 1190, 1583
BspT107I GGYRCC 1 cut(s) 1124
BsrBI CCGCTC 1 cut(s) 294
BsrGI TGTACA 1 cut(s) 735
BsrI ACTGG 7 cut(s) 184, 383, 725, 1059, 1078, 1138, 1233
BssECI CCNNGG 3 cut(s) 70, 138, 545
BssMI GATC 5 cut(s) 818, 1084, 1171, 1195, 1575
BssT1I CCWWGG 2 cut(s) 70, 545
Bst4CI ACNGT 5 cut(s) 527, 1129, 1246, 1309, 1496
BstAUI TGTACA 1 cut(s) 735
BstC8I GCNNGC 1 cut(s) 946
BstDEI CTNAG 8 cut(s) 153, 501, 624, 710, 857, 1326, 1351, 1461
BstEII GGTNACC 1 cut(s) 714
BstF5I GGATG 5 cut(s) 410, 490, 541, 870, 1126
BstH2I RGCGCY 1 cut(s) 1518
BstHHI GCGC 1 cut(s) 1517
BstKTI GATC 5 cut(s) 821, 1087, 1174, 1198, 1578
BstMAI GTCTC 4 cut(s) 96, 293, 1381, 1464
BstMBI GATC 5 cut(s) 818, 1084, 1171, 1195, 1575
BstMWI GCNNNNNNNGC 6 cut(s) 343, 662, 977, 989, 1122, 1412
BstPI GGTNACC 1 cut(s) 714
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 651
BstSLI GKGCMC 1 cut(s) 100
BstV1I GCAGC 3 cut(s) 665, 967, 1439
BstV2I GAAGAC 2 cut(s) 585, 1497
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 3 cut(s) 5, 98, 143
BtsCI GGATG 5 cut(s) 410, 490, 541, 870, 1126
BtsIMutI CAGTG 2 cut(s) 1052, 1226
BveI ACCTGC 1 cut(s) 1372
Cac8I GCNNGC 1 cut(s) 946
CciI TCATGA 1 cut(s) 876
CfoI GCGC 1 cut(s) 1517
Cfr13I GGNCC 3 cut(s) 96, 97, 319
CseI GACGC 2 cut(s) 835, 889
Csp6I GTAC 2 cut(s) 736, 898
CviAII CATG 5 cut(s) 696, 850, 877, 887, 1216
CviQI GTAC 2 cut(s) 736, 898
DdeI CTNAG 8 cut(s) 153, 501, 624, 710, 857, 1326, 1351, 1461
DpnI GATC 5 cut(s) 820, 1086, 1173, 1197, 1577
DpnII GATC 5 cut(s) 818, 1084, 1171, 1195, 1575
DrdI GACNNNNNNGTC 1 cut(s) 1483
DriI GACNNNNNGTC 1 cut(s) 1456
DseDI GACNNNNNNGTC 1 cut(s) 1483
Eam1105I GACNNNNNGTC 1 cut(s) 1456
EciI GGCGGA 1 cut(s) 229
Ecl136II GAGCTC 2 cut(s) 82, 310
Eco130I CCWWGG 2 cut(s) 70, 545
Eco24I GRGCYC 3 cut(s) 84, 100, 312
Eco32I GATATC 1 cut(s) 1332
Eco47I GGWCC 1 cut(s) 319
Eco47III AGCGCT 1 cut(s) 1516
Eco53kI GAGCTC 2 cut(s) 82, 310
Eco57I CTGAAG 1 cut(s) 1509
Eco88I CYCGRG 2 cut(s) 93, 1292
Eco91I GGTNACC 1 cut(s) 714
EcoICRI GAGCTC 2 cut(s) 82, 310
EcoO109I RGGNCCY 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 714
EcoRV GATATC 1 cut(s) 1332
EcoT14I CCWWGG 2 cut(s) 70, 545
EcoT22I ATGCAT 1 cut(s) 928
EcoT38I GRGCYC 3 cut(s) 84, 100, 312
ErhI CCWWGG 2 cut(s) 70, 545
Esp3I CGTCTC 2 cut(s) 96, 1381
FaeI CATG 5 cut(s) 699, 853, 880, 890, 1219
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 1 cut(s) 154
FatI CATG 5 cut(s) 695, 849, 876, 886, 1215
FauNDI CATATG 1 cut(s) 1360
Fnu4HI GCNGC 5 cut(s) 149, 654, 981, 984, 1428
FokI GGATG 5 cut(s) 397, 497, 528, 857, 1133
FriOI GRGCYC 3 cut(s) 84, 100, 312
Fsp4HI GCNGC 5 cut(s) 149, 654, 981, 984, 1428
FspBI CTAG 5 cut(s) 71, 546, 782, 816, 1412
GlaI GCGC 1 cut(s) 1516
GluI GCNGC 5 cut(s) 149, 654, 981, 984, 1428
GsuI CTGGAG 2 cut(s) 167, 1053
HaeII RGCGCY 1 cut(s) 1518
HaeIII GGCC 3 cut(s) 5, 98, 143
HapII CCGG 1 cut(s) 140
HgaI GACGC 2 cut(s) 835, 889
HhaI GCGC 1 cut(s) 1517
Hin1II CATG 5 cut(s) 699, 853, 880, 890, 1219
Hin6I GCGC 1 cut(s) 1515
HinP1I GCGC 1 cut(s) 1515
HindIII AAGCTT 2 cut(s) 50, 467
HinfI GANTC 4 cut(s) 167, 592, 1451, 1457
HpaII CCGG 1 cut(s) 140
HphI GGTGA 6 cut(s) 187, 372, 581, 1079, 1244, 1396
Hpy166II GTNNAC 4 cut(s) 370, 611, 736, 1226
Hpy188III TCNNGA 6 cut(s) 482, 517, 877, 1032, 1088, 1294
Hpy8I GTNNAC 4 cut(s) 370, 611, 736, 1226
Hpy99I CGWCG 4 cut(s) 167, 263, 308, 329
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 5 cut(s) 527, 1129, 1246, 1309, 1496
HpyCH4IV ACGT 3 cut(s) 377, 642, 1478
HpyCH4V TGCA 5 cut(s) 488, 653, 926, 971, 1052
HpyF10VI GCNNNNNNNGC 6 cut(s) 343, 662, 977, 989, 1122, 1412
HpyF3I CTNAG 8 cut(s) 153, 501, 624, 710, 857, 1326, 1351, 1461
HpySE526I ACGT 3 cut(s) 377, 642, 1478
Hsp92II CATG 5 cut(s) 699, 853, 880, 890, 1219
HspAI GCGC 1 cut(s) 1515
Kzo9I GATC 5 cut(s) 818, 1084, 1171, 1195, 1575
LmnI GCTCC 8 cut(s) 87, 126, 257, 299, 538, 1034, 1411, 1435
Lsp1109I GCAGC 3 cut(s) 665, 967, 1439
LweI GCATC 3 cut(s) 475, 550, 931
MaeI CTAG 5 cut(s) 71, 546, 782, 816, 1412
MaeII ACGT 3 cut(s) 377, 642, 1478
MaeIII GTNAC 5 cut(s) 378, 714, 1129, 1250, 1287
MalI GATC 5 cut(s) 820, 1086, 1173, 1197, 1577
MbiI CCGCTC 1 cut(s) 294
MboI GATC 5 cut(s) 818, 1084, 1171, 1195, 1575
MboII GAAGA 6 cut(s) 184, 590, 956, 1404, 1502, 1562
MhlI GDGCHC 3 cut(s) 84, 100, 312
MluCI AATT 4 cut(s) 357, 677, 683, 1147
MlyI GAGTC 3 cut(s) 176, 1445, 1466
MmeI TCCRAC 4 cut(s) 81, 1318, 1454, 1457
Mph1103I ATGCAT 1 cut(s) 928
MseI TTAA 4 cut(s) 680, 812, 950, 1588
MslI CAYNNNNRTG 1 cut(s) 875
MspA1I CMGCKG 1 cut(s) 1573
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 1550
MwoI GCNNNNNNNGC 6 cut(s) 343, 662, 977, 989, 1122, 1412
NciI CCSGG 1 cut(s) 140
NdeI CATATG 1 cut(s) 1360
NdeII GATC 5 cut(s) 818, 1084, 1171, 1195, 1575
NlaIII CATG 5 cut(s) 699, 853, 880, 890, 1219
NlaIV GGNNCC 4 cut(s) 98, 253, 1126, 1407
NmuCI GTSAC 3 cut(s) 378, 1250, 1287
NsiI ATGCAT 1 cut(s) 928
PaeR7I CTCGAG 1 cut(s) 1292
PagI TCATGA 1 cut(s) 876
PaqCI CACCTGC 1 cut(s) 1372
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 309
PctI GAATGC 1 cut(s) 1550
PfeI GAWTC 1 cut(s) 592
PkrI GCNGC 5 cut(s) 150, 655, 982, 985, 1429
PleI GAGTC 3 cut(s) 175, 1445, 1465
PpsI GAGTC 3 cut(s) 175, 1445, 1465
PpuMI RGGWCCY 1 cut(s) 319
Psp124BI GAGCTC 2 cut(s) 84, 312
Psp5II RGGWCCY 1 cut(s) 319
PspEI GGTNACC 1 cut(s) 714
PspN4I GGNNCC 4 cut(s) 98, 253, 1126, 1407
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 3 cut(s) 96, 97, 319
PspPPI RGGWCCY 1 cut(s) 319
PstI CTGCAG 1 cut(s) 655
RsaI GTAC 2 cut(s) 737, 899
RsaNI GTAC 2 cut(s) 736, 898
RseI CAYNNNNRTG 1 cut(s) 875
SacI GAGCTC 2 cut(s) 84, 312
SaqAI TTAA 4 cut(s) 680, 812, 950, 1588
SatI GCNGC 5 cut(s) 149, 654, 981, 984, 1428
Sau3AI GATC 5 cut(s) 818, 1084, 1171, 1195, 1575
Sau96I GGNCC 3 cut(s) 96, 97, 319
SchI GAGTC 3 cut(s) 176, 1445, 1466
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 3 cut(s) 84, 100, 312
SfaNI GCATC 3 cut(s) 475, 550, 931
SfcI CTRYAG 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 1292
SinI GGWCC 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 1292
SmiMI CAYNNNNRTG 1 cut(s) 875
SmlI CTYRAG 2 cut(s) 362, 1292
SmoI CTYRAG 2 cut(s) 362, 1292
Sse9I AATT 4 cut(s) 357, 677, 683, 1147
SsiI CCGC 6 cut(s) 149, 240, 292, 983, 1065, 1573
SspI AATATT 2 cut(s) 661, 1371
SspMI CTAG 5 cut(s) 71, 546, 782, 816, 1412
SstI GAGCTC 2 cut(s) 84, 312
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 2 cut(s) 70, 545
TaaI ACNGT 5 cut(s) 527, 1129, 1246, 1309, 1496
TaiI ACGT 3 cut(s) 380, 645, 1481
TaqI TCGA 4 cut(s) 165, 303, 327, 1293
TasI AATT 4 cut(s) 357, 677, 683, 1147
TatI WGTACW 1 cut(s) 735
TauI GCSGC 2 cut(s) 151, 986
TfiI GAWTC 1 cut(s) 592
Tru1I TTAA 4 cut(s) 680, 812, 950, 1588
Tru9I TTAA 4 cut(s) 680, 812, 950, 1588
TscAI CASTG 2 cut(s) 1059, 1233
TseFI GTSAC 3 cut(s) 378, 1250, 1287
TseI GCWGC 3 cut(s) 653, 980, 1427
Tsp45I GTSAC 3 cut(s) 378, 1250, 1287
TspDTI ATGAA 8 cut(s) 262, 369, 399, 591, 689, 859, 1200, 1347
TspGWI ACGGA 4 cut(s) 428, 1133, 1238, 1324
TspRI CASTG 2 cut(s) 1059, 1233
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 2 cut(s) 357, 683
XhoI CTCGAG 1 cut(s) 1292
XmaJI CCTAGG 2 cut(s) 70, 545
XspI CTAG 5 cut(s) 71, 546, 782, 816, 1412
Zsp2I ATGCAT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.