MD10G1141700.v1.1

Nucleolar complex protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003349
Physical Location & Seq
Forward (+)
22756804 .. 22762247
5444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1141700.v1.1.491

Sequence Viewer

Length: 1857 bp
ATGGCCTCCATCCTCTCCAAGAAGCAGAAGCAGAAGCAAAAAAGCACCAAGCTTTCCGACCTAAAAACACTAGGGAACCAGCTCCTCTCGTCCCGGGCCCACATCAACAACCTCCCGAAGCTCCTCTCCTTCGTCTCCCCGGCCTCGCCGCCTCAGTTCGTCGTCGAGTCCCTCCTCTCTCTCCAGTCCTTTTTTACCCCTCTCCTCCCCGACCTACCTCCCTCTTCCTCCAAGCCTTCCGCCGCCGATGGCTCCCACGACGACCCCGACTTCATATACCGCACCTGGCTCCGCTCCAAGTTCGACGAGCTCGTTGAGGCCCTCGTCGAAGTCCTGCTTTCGCCGCAATCTGACGAAACTCTCAAGGAGGTGGTGTTGGACACGATAATGGAGTTTGTGAAGTTCGGAAATGGCGGAAAGTTTCACTCTGCAATGTACCATAGGCTGCTTCGAAGTATTGTTTACACTACGGAACCGGTTAATTTGTTGTTAGACTTGCTCTCATCCAAGTATTTCAAGTATATCGATGTCCGTTATTTTACCTACATTAGCTTGGAAAAGCTTGCTAAAACTCTGGATGCAAATGGCATTTCTGAGGACAAAAATGTGAAGAAAGACGGCAATGATGGGGAGCATCCCAGAGAAAGCATGGATCAATTAATTCACAAGATTCATTATTTGATATCACACATCCCCTCTCTGGAAAGCTCAGTTGAAAAAACTGATCATGAGATGTGGAGTGGATCAGGTGAATATGAAGAGCTTGCTGGGAAGATGAAGGCAGATGGTAAACAGACTGAGAAGCACAAGGATAAGGTGTTGTCTGCAGACAATATTGCCAAAAAGATGAAATTGAAATTTACTAAAGCATGGTTATCATTTCTAAGGTTACCACTTCCAATTGATGTGTACAAGGAGGTTCTTGCCACTCTCCATCAGGCAGTCATTCCTTTTCTAGCCAACCCCGTCTTGTTATGTGACTTCTTAACTCGATCATATGACATTGGTGGTGTTATTAGTGTCATGGCTCTTAGCAGTCTCTTTATCCTCATGACAAAACATGGTCTAGAGTACCCCAACTTTTATGAGAAACTTTATGCATTACTGGCTCCCTCTATTTTTATGGCAAAGCATCGATCAAAGTTTTTTCAGCTTGTAGATGCTTGCTTAAAATCACCACTTCTTCCTGCATATTTGGCTGCTGCTTTTGCCAAGAAATTGAGTAGGCTGTCAATCTCAGTTCCTCCGTCTGGAGCATTAGTTATTATTGCACTGGTTCATAATCTTCTACGTAGACATCCTTCAATCAATTGCTTGGTGAATCGGGGAGGTGGTGTTGAAACTGTGAAAGATGATCCAGAAGCAGAACAGAGGGCTGCTGATGGTGCTGATGGCACTGGAACTGGTTCGGCAGATAAGTCAATCAAAAAATCAGGCATTGATCCTTTTGATAATGAACAAAGTGATCCTATGAAATCAAATGCCATGAGAAGCTCACTTTGGGAGATTGATACCCTCCGTCACCACTACTGCCCTGCTGTTTCAAGGTTTGTTTTATCACTCGAGAATGATTTGACAGTCAGAGCCAAAACCACTGAAATATCTGTTGGAGATTTTAGCTCAGGTTCATATGCGACAATATTTGGAGAGCAGGTGAGACGAAGGATAAAGTCGGCTCCACTAGCATACTACAAAGCAGCTCCGACCTCTTTGTTCTCAGATTCCGAGTCTCAGTTTCTCGGTTGGACTTTCACGTGTGAAGACAGTAAGCGAAAGAACGACAGCGCTGATGAGAATGGGAAAATAGATAAAGAATGTGACCATAGTCCGGCCAAACGACAACGCGTCAAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

619

Amino Acids

69.01

Weight (kDa)

8.52

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 340 - 518 1.2e-46 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1642
Acc36I ACCTGC 1 cut(s) 1642
AccBSI CCGCTC 1 cut(s) 294
AccI GTMKAC 1 cut(s) 1294
AccII CGCG 1 cut(s) 1845
AciI CCGC 7 cut(s) 149, 240, 243, 280, 292, 344, 414
AclWI GGATC 5 cut(s) 660, 751, 1349, 1436, 1460
AcoI YGGCCR 1 cut(s) 1830
AcsI RAATTY 1 cut(s) 857
AcvI CACGTG 1 cut(s) 1755
AfaI GTAC 3 cut(s) 437, 911, 1073
AfeI AGCGCT 1 cut(s) 1786
AfiI CCNNNNNNNGG 3 cut(s) 700, 1250, 1828
AflIII ACRYGT 2 cut(s) 1754, 1843
AgeI ACCGGT 1 cut(s) 475
AgsI TTSAA 6 cut(s) 517, 716, 856, 1305, 1340, 1545
AhdI GACNNNNNGTC 1 cut(s) 1844
AjnI CCWGG 1 cut(s) 284
AjuI GAANNNNNNNTTGG 4 cut(s) 1483, 1515, 1590, 1622
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 4 cut(s) 139, 1043, 1651, 1734
AlwI GGATC 5 cut(s) 660, 751, 1349, 1436, 1460
Ama87I CYCGRG 2 cut(s) 93, 1562
Aor51HI AGCGCT 1 cut(s) 1786
AoxI GGCC 5 cut(s) 3, 96, 141, 318, 1830
ApaI GGGCCC 1 cut(s) 100
ApeKI GCWGC 5 cut(s) 445, 1199, 1202, 1376, 1697
ApoI RAATTY 1 cut(s) 857
AseI ATTAAT 1 cut(s) 659
AsiGI ACCGGT 1 cut(s) 475
Asp700I GAANNNNTTC 1 cut(s) 1405
AspLEI GCGC 1 cut(s) 1787
AspS9I GGNCC 3 cut(s) 96, 97, 319
AsuC2I CCSGG 3 cut(s) 94, 95, 140
AsuHPI GGTGA 5 cut(s) 761, 1167, 1330, 1514, 1666
AsuII TTCGAA 1 cut(s) 451
AvaI CYCGRG 2 cut(s) 93, 1562
BaeGI GKGCMC 1 cut(s) 100
BanII GRGCYC 2 cut(s) 100, 312
BarI GAAGNNNNNNTAC 2 cut(s) 1285, 1317
BbrPI CACGTG 1 cut(s) 1755
BbsI GAAGAC 1 cut(s) 1767
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 5 cut(s) 432, 1186, 1189, 1363, 1709
BccI CCATC 7 cut(s) 17, 242, 620, 779, 942, 1376, 1385
BceAI ACGGC 1 cut(s) 634
BciT130I CCWGG 1 cut(s) 286
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 3 cut(s) 94, 95, 140
BcoDI GTCTC 4 cut(s) 139, 1043, 1651, 1734
BfaI CTAG 4 cut(s) 71, 956, 1067, 1682
BfmI CTRYAG 1 cut(s) 825
BfoI RGCGCY 1 cut(s) 1788
BfuAI ACCTGC 1 cut(s) 1642
BisI GCNGC 8 cut(s) 149, 243, 344, 446, 1200, 1203, 1377, 1698
BlsI GCNGC 8 cut(s) 150, 244, 345, 447, 1201, 1204, 1378, 1699
Bme1390I CCNGG 4 cut(s) 94, 95, 140, 286
BmeRI GACNNNNNGTC 1 cut(s) 1844
BmeT110I CYCGRG 2 cut(s) 93, 1562
BmgT120I GGNCC 3 cut(s) 96, 97, 319
BmiI GGNNCC 7 cut(s) 77, 98, 253, 290, 474, 1110, 1677
BmrFI CCNGG 4 cut(s) 94, 95, 140, 286
BmsI GCATC 4 cut(s) 568, 643, 1141, 1150
BoxI GACNNNNGTC 1 cut(s) 1824
BpiI GAAGAC 1 cut(s) 1767
BpmI CTGGAG 2 cut(s) 167, 1272
Bpu10I CCTNAGC 1 cut(s) 1621
Bpu14I TTCGAA 1 cut(s) 451
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 3 cut(s) 94, 95, 140
Bsa29I ATCGAT 2 cut(s) 525, 1135
BsaAI YACGTR 2 cut(s) 1292, 1755
BsaJI CCNNGG 2 cut(s) 93, 138
BsaWI WCCGGW 1 cut(s) 475
BsaXI ACNNNNNCTCC 8 cut(s) 359, 389, 1245, 1275, 1320, 1350, 1638, 1668
Bsc4I CCNNNNNNNGG 3 cut(s) 700, 1250, 1828
Bse118I RCCGGY 1 cut(s) 475
Bse1I ACTGG 5 cut(s) 184, 1110, 1278, 1402, 1408
Bse3DI GCAATG 2 cut(s) 438, 628
BseBI CCWGG 1 cut(s) 286
BseCI ATCGAT 2 cut(s) 525, 1135
BseDI CCNNGG 2 cut(s) 93, 138
BseGI GGATG 6 cut(s) 9, 503, 583, 634, 690, 1297
BseLI CCNNNNNNNGG 3 cut(s) 700, 1250, 1828
BseMI GCAATG 2 cut(s) 438, 628
BseMII CTCAG 8 cut(s) 167, 585, 723, 789, 1251, 1635, 1731, 1745
BseNI ACTGG 5 cut(s) 184, 1110, 1278, 1402, 1408
BseRI GAGGAG 4 cut(s) 74, 113, 164, 194
BseSI GKGCMC 1 cut(s) 100
BseXI GCAGC 5 cut(s) 432, 1186, 1189, 1363, 1709
BseYI CCCAGC 1 cut(s) 767
Bsh1236I CGCG 1 cut(s) 1845
BshFI GGCC 5 cut(s) 5, 98, 143, 320, 1832
BshTI ACCGGT 1 cut(s) 475
BshVI ATCGAT 2 cut(s) 525, 1135
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 2 cut(s) 93, 1562
BsiSI CCGG 4 cut(s) 94, 140, 476, 1829
BslFI GGGAC 2 cut(s) 76, 154
BslI CCNNNNNNNGG 3 cut(s) 700, 1250, 1828
BsmAI GTCTC 4 cut(s) 139, 1043, 1651, 1734
BsmBI CGTCTC 2 cut(s) 139, 1651
BsmFI GGGAC 2 cut(s) 76, 154
BsnI GGCC 5 cut(s) 5, 98, 143, 320, 1832
BsoBI CYCGRG 2 cut(s) 93, 1562
Bsp119I TTCGAA 1 cut(s) 451
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 2 cut(s) 100, 312
Bsp1407I TGTACA 1 cut(s) 909
Bsp143I GATC 8 cut(s) 652, 724, 743, 992, 1136, 1354, 1441, 1465
BspACI CCGC 7 cut(s) 149, 240, 243, 280, 292, 344, 414
BspANI GGCC 5 cut(s) 5, 98, 143, 320, 1832
BspCNI CTCAG 8 cut(s) 166, 586, 722, 790, 1250, 1634, 1730, 1744
BspDI ATCGAT 2 cut(s) 525, 1135
BspFNI CGCG 1 cut(s) 1845
BspHI TCATGA 2 cut(s) 727, 1050
BspLI GGNNCC 7 cut(s) 77, 98, 253, 290, 474, 1110, 1677
BspMAI CTGCAG 1 cut(s) 829
BspMI ACCTGC 1 cut(s) 1642
BspPI GGATC 5 cut(s) 660, 751, 1349, 1436, 1460
BspQI GCTCTTC 1 cut(s) 753
BspT104I TTCGAA 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 294
BsrDI GCAATG 2 cut(s) 438, 628
BsrFI RCCGGY 1 cut(s) 475
BsrGI TGTACA 1 cut(s) 909
BsrI ACTGG 5 cut(s) 184, 1110, 1278, 1402, 1408
BssAI RCCGGY 1 cut(s) 475
BssECI CCNNGG 2 cut(s) 93, 138
BssMI GATC 8 cut(s) 652, 724, 743, 992, 1136, 1354, 1441, 1465
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 3 cut(s) 1345, 1579, 1766
Bst6I CTCTTC 2 cut(s) 229, 753
BstAUI TGTACA 1 cut(s) 909
BstBAI YACGTR 2 cut(s) 1292, 1755
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 3 cut(s) 564, 765, 1165
BstEII GGTNACC 1 cut(s) 888
BstF5I GGATG 6 cut(s) 9, 503, 583, 634, 690, 1297
BstFNI CGCG 1 cut(s) 1845
BstH2I RGCGCY 1 cut(s) 1788
BstHHI GCGC 1 cut(s) 1787
BstKTI GATC 8 cut(s) 655, 727, 746, 995, 1139, 1357, 1444, 1468
BstMAI GTCTC 4 cut(s) 139, 1043, 1651, 1734
BstMBI GATC 8 cut(s) 652, 724, 743, 992, 1136, 1354, 1441, 1465
BstMWI GCNNNNNNNGC 7 cut(s) 343, 1106, 1196, 1208, 1385, 1682, 1851
BstNI CCWGG 1 cut(s) 286
BstPAI GACNNNNGTC 1 cut(s) 1824
BstPI GGTNACC 1 cut(s) 888
BstSCI CCNGG 4 cut(s) 92, 93, 138, 284
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 1 cut(s) 100
BstSNI TACGTA 1 cut(s) 1292
BstUI CGCG 1 cut(s) 1845
BstV1I GCAGC 5 cut(s) 432, 1186, 1189, 1363, 1709
BstV2I GAAGAC 1 cut(s) 1767
Bsu15I ATCGAT 2 cut(s) 525, 1135
BsuRI GGCC 5 cut(s) 5, 98, 143, 320, 1832
BsuTUI ATCGAT 2 cut(s) 525, 1135
BtsCI GGATG 6 cut(s) 9, 503, 583, 634, 690, 1297
BtsIMutI CAGTG 3 cut(s) 1271, 1395, 1593
BveI ACCTGC 1 cut(s) 1642
Cac8I GCNNGC 3 cut(s) 564, 765, 1165
CciI TCATGA 2 cut(s) 727, 1050
CfoI GCGC 1 cut(s) 1787
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 3 cut(s) 96, 97, 319
Cfr9I CCCGGG 1 cut(s) 93
ClaI ATCGAT 2 cut(s) 525, 1135
CseI GACGC 1 cut(s) 1834
Csp6I GTAC 3 cut(s) 436, 910, 1072
CspAI ACCGGT 1 cut(s) 475
CviAII CATG 7 cut(s) 649, 728, 870, 1024, 1051, 1061, 1486
CviQI GTAC 3 cut(s) 436, 910, 1072
DpnI GATC 8 cut(s) 654, 726, 745, 994, 1138, 1356, 1443, 1467
DpnII GATC 8 cut(s) 652, 724, 743, 992, 1136, 1354, 1441, 1465
DriI GACNNNNNGTC 1 cut(s) 1844
EaeI YGGCCR 1 cut(s) 1830
Eam1104I CTCTTC 2 cut(s) 229, 753
Eam1105I GACNNNNNGTC 1 cut(s) 1844
EarI CTCTTC 2 cut(s) 229, 753
EciI GGCGGA 2 cut(s) 229, 429
Ecl136II GAGCTC 1 cut(s) 310
Eco105I TACGTA 1 cut(s) 1292
Eco24I GRGCYC 2 cut(s) 100, 312
Eco32I GATATC 1 cut(s) 684
Eco47III AGCGCT 1 cut(s) 1786
Eco53kI GAGCTC 1 cut(s) 310
Eco72I CACGTG 1 cut(s) 1755
Eco88I CYCGRG 2 cut(s) 93, 1562
Eco91I GGTNACC 1 cut(s) 888
EcoICRI GAGCTC 1 cut(s) 310
EcoO109I RGGNCCY 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 888
EcoRII CCWGG 1 cut(s) 284
EcoRV GATATC 1 cut(s) 684
EcoT22I ATGCAT 1 cut(s) 1102
EcoT38I GRGCYC 2 cut(s) 100, 312
Esp3I CGTCTC 2 cut(s) 139, 1651
FaeI CATG 7 cut(s) 652, 731, 873, 1027, 1054, 1064, 1489
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 2 cut(s) 76, 154
FatI CATG 7 cut(s) 648, 727, 869, 1023, 1050, 1060, 1485
FauNDI CATATG 2 cut(s) 997, 1630
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 1294
Fnu4HI GCNGC 8 cut(s) 149, 243, 344, 446, 1200, 1203, 1377, 1698
FokI GGATG 5 cut(s) 490, 590, 621, 677, 1284
FriOI GRGCYC 2 cut(s) 100, 312
Fsp4HI GCNGC 8 cut(s) 149, 243, 344, 446, 1200, 1203, 1377, 1698
FspBI CTAG 4 cut(s) 71, 956, 1067, 1682
GlaI GCGC 1 cut(s) 1786
GluI GCNGC 8 cut(s) 149, 243, 344, 446, 1200, 1203, 1377, 1698
GsaI CCCAGC 1 cut(s) 771
GsuI CTGGAG 2 cut(s) 167, 1272
HaeII RGCGCY 1 cut(s) 1788
HaeIII GGCC 5 cut(s) 5, 98, 143, 320, 1832
HapII CCGG 4 cut(s) 94, 140, 476, 1829
HgaI GACGC 1 cut(s) 1834
HhaI GCGC 1 cut(s) 1787
Hin1II CATG 7 cut(s) 652, 731, 873, 1027, 1054, 1064, 1489
Hin6I GCGC 1 cut(s) 1785
HinP1I GCGC 1 cut(s) 1785
HindIII AAGCTT 2 cut(s) 50, 560
HinfI GANTC 5 cut(s) 167, 670, 1321, 1721, 1727
HpaII CCGG 4 cut(s) 94, 140, 476, 1829
HphI GGTGA 5 cut(s) 761, 1167, 1330, 1514, 1666
Hpy166II GTNNAC 4 cut(s) 463, 791, 910, 1295
Hpy188I TCNGA 8 cut(s) 58, 352, 407, 595, 1583, 1704, 1720, 1726
Hpy188III TCNNGA 9 cut(s) 115, 575, 701, 728, 1051, 1067, 1251, 1358, 1564
Hpy8I GTNNAC 4 cut(s) 463, 791, 910, 1295
Hpy99I CGWCG 5 cut(s) 164, 167, 263, 308, 329
HpyAV CCTTC 5 cut(s) 139, 246, 772, 1311, 1656
HpyCH4III ACNGT 3 cut(s) 1345, 1579, 1766
HpyCH4IV ACGT 2 cut(s) 1291, 1754
HpyCH4V TGCA 6 cut(s) 431, 581, 827, 1100, 1190, 1271
HpyF10VI GCNNNNNNNGC 7 cut(s) 343, 1106, 1196, 1208, 1385, 1682, 1851
HpySE526I ACGT 2 cut(s) 1291, 1754
Hsp92II CATG 7 cut(s) 652, 731, 873, 1027, 1054, 1064, 1489
HspAI GCGC 1 cut(s) 1785
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 8 cut(s) 652, 724, 743, 992, 1136, 1354, 1441, 1465
LguI GCTCTTC 1 cut(s) 753
Lsp1109I GCAGC 5 cut(s) 432, 1186, 1189, 1363, 1709
LweI GCATC 4 cut(s) 568, 643, 1141, 1150
MaeI CTAG 4 cut(s) 71, 956, 1067, 1682
MaeII ACGT 2 cut(s) 1291, 1754
MaeIII GTNAC 4 cut(s) 888, 977, 1520, 1817
MalI GATC 8 cut(s) 654, 726, 745, 994, 1138, 1356, 1443, 1467
MbiI CCGCTC 1 cut(s) 294
MboI GATC 8 cut(s) 652, 724, 743, 992, 1136, 1354, 1441, 1465
MboII GAAGA 7 cut(s) 216, 622, 770, 784, 1175, 1277, 1772
MfeI CAATTG 2 cut(s) 900, 1309
MhlI GDGCHC 2 cut(s) 100, 312
MluCI AATT 8 cut(s) 481, 656, 660, 851, 857, 900, 1217, 1309
MluI ACGCGT 1 cut(s) 1843
MlyI GAGTC 2 cut(s) 176, 1736
MmeI TCCRAC 5 cut(s) 81, 357, 1588, 1724, 1727
Mph1103I ATGCAT 1 cut(s) 1102
MroXI GAANNNNTTC 1 cut(s) 1405
MseI TTAA 4 cut(s) 480, 659, 986, 1169
MslI CAYNNNNRTG 1 cut(s) 386
MspI CCGG 4 cut(s) 94, 140, 476, 1829
MspR9I CCNGG 4 cut(s) 94, 95, 140, 286
MunI CAATTG 2 cut(s) 900, 1309
MvaI CCWGG 1 cut(s) 286
MvnI CGCG 1 cut(s) 1845
MwoI GCNNNNNNNGC 7 cut(s) 343, 1106, 1196, 1208, 1385, 1682, 1851
NciI CCSGG 3 cut(s) 94, 95, 140
NdeI CATATG 2 cut(s) 997, 1630
NdeII GATC 8 cut(s) 652, 724, 743, 992, 1136, 1354, 1441, 1465
NlaIII CATG 7 cut(s) 652, 731, 873, 1027, 1054, 1064, 1489
NlaIV GGNNCC 7 cut(s) 77, 98, 253, 290, 474, 1110, 1677
NmuCI GTSAC 3 cut(s) 977, 1520, 1817
NsiI ATGCAT 1 cut(s) 1102
NspV TTCGAA 1 cut(s) 451
PaeR7I CTCGAG 1 cut(s) 1562
PagI TCATGA 2 cut(s) 727, 1050
PaqCI CACCTGC 1 cut(s) 1642
PciSI GCTCTTC 1 cut(s) 753
PcsI WCGNNNNNNNCGW 2 cut(s) 264, 309
PdmI GAANNNNTTC 1 cut(s) 1405
PfeI GAWTC 3 cut(s) 670, 1321, 1721
PinAI ACCGGT 1 cut(s) 475
PkrI GCNGC 8 cut(s) 150, 244, 345, 447, 1201, 1204, 1378, 1699
PleI GAGTC 2 cut(s) 175, 1735
PmaCI CACGTG 1 cut(s) 1755
PmlI CACGTG 1 cut(s) 1755
PpsI GAGTC 2 cut(s) 175, 1735
Ppu21I YACGTR 2 cut(s) 1292, 1755
PshAI GACNNNNGTC 1 cut(s) 1824
PshBI ATTAAT 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 284
PspCI CACGTG 1 cut(s) 1755
PspEI GGTNACC 1 cut(s) 888
PspFI CCCAGC 1 cut(s) 767
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 7 cut(s) 77, 98, 253, 290, 474, 1110, 1677
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 3 cut(s) 96, 97, 319
PstI CTGCAG 1 cut(s) 829
RsaI GTAC 3 cut(s) 437, 911, 1073
RsaNI GTAC 3 cut(s) 436, 910, 1072
RseI CAYNNNNRTG 1 cut(s) 386
SacI GAGCTC 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 4 cut(s) 480, 659, 986, 1169
SatI GCNGC 8 cut(s) 149, 243, 344, 446, 1200, 1203, 1377, 1698
Sau3AI GATC 8 cut(s) 652, 724, 743, 992, 1136, 1354, 1441, 1465
Sau96I GGNCC 3 cut(s) 96, 97, 319
SchI GAGTC 2 cut(s) 176, 1736
ScrFI CCNGG 4 cut(s) 94, 95, 140, 286
SduI GDGCHC 2 cut(s) 100, 312
SfaNI GCATC 4 cut(s) 568, 643, 1141, 1150
SfcI CTRYAG 1 cut(s) 825
Sfr274I CTCGAG 1 cut(s) 1562
SfuI TTCGAA 1 cut(s) 451
SlaI CTCGAG 1 cut(s) 1562
SmaI CCCGGG 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 2 cut(s) 362, 1562
SmoI CTYRAG 2 cut(s) 362, 1562
SnaBI TACGTA 1 cut(s) 1292
Sse9I AATT 8 cut(s) 481, 656, 660, 851, 857, 900, 1217, 1309
SsiI CCGC 7 cut(s) 149, 240, 243, 280, 292, 344, 414
SspI AATATT 2 cut(s) 835, 1641
SspMI CTAG 4 cut(s) 71, 956, 1067, 1682
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 4 cut(s) 92, 93, 138, 284
TaaI ACNGT 3 cut(s) 1345, 1579, 1766
TaiI ACGT 2 cut(s) 1294, 1757
TaqI TCGA 8 cut(s) 165, 303, 327, 451, 525, 991, 1135, 1563
TasI AATT 8 cut(s) 481, 656, 660, 851, 857, 900, 1217, 1309
TatI WGTACW 1 cut(s) 909
TauI GCSGC 3 cut(s) 151, 245, 346
TfiI GAWTC 3 cut(s) 670, 1321, 1721
Tru1I TTAA 4 cut(s) 480, 659, 986, 1169
Tru9I TTAA 4 cut(s) 480, 659, 986, 1169
TscAI CASTG 3 cut(s) 1278, 1402, 1600
TseFI GTSAC 3 cut(s) 977, 1520, 1817
TseI GCWGC 5 cut(s) 445, 1199, 1202, 1376, 1697
Tsp45I GTSAC 3 cut(s) 977, 1520, 1817
TspDTI ATGAA 9 cut(s) 262, 662, 771, 791, 863, 1268, 1470, 1487, 1617
TspGWI ACGGA 4 cut(s) 485, 521, 1236, 1508
TspMI CCCGGG 1 cut(s) 93
TspRI CASTG 3 cut(s) 1278, 1402, 1600
VspI ATTAAT 1 cut(s) 659
XapI RAATTY 1 cut(s) 857
XbaI TCTAGA 1 cut(s) 1066
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XhoI CTCGAG 1 cut(s) 1562
XmaI CCCGGG 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 1294
XmnI GAANNNNTTC 1 cut(s) 1405
XspI CTAG 4 cut(s) 71, 956, 1067, 1682
Zsp2I ATGCAT 1 cut(s) 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.