Rh6DG243400

Nucleolar complex protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003349
Physical Location & Seq
Forward (+)
43046117 .. 43050462
4346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG243400.1

Sequence Viewer

Length: 1329 bp
ATGTTGGAGGTCATGAATTATTGTTTAATTGGCAAAGGTGTTAGATTCCTTAATAAGTTGATGCCGCAACTTGTTAGCTTTAGAATTCTTAGATTTGTTTACTTTTCTTGCAGCATGGAGCAATTAATTCGCAATATTCATTACATTATGTCACATATCCCCGCTTTTGAAGGTTCAGTTGAAAAAACTGATTATGACATGTGGAGTGGATCAAGTGAATCTGAAGGGCATTCTGAGAGCCAGAAATCAAAAGATAAAAAGAAGACTGAGAAAGATAACGATAAGGCGTTGTCTGCAGCCAATATTGTCAAAAAGATGAAATTAAAATTTACTAAAGCATGGTTATCATTTCTAAGGTTACCACTTCCTCTTGATGTGTACAAGGAGGTTCTTGCCAGCCTTCATCAGGCAGTCATTCCTTATATATCTAACCCCGTTGTGTTATGTGACTTCTTAACTAGATCATATGACATTGGTGGTGTTATCAGCGTCATGGCTCTTATCAGTCTCTTCATCATTATGACAAAATATGGTTTAGAGTACCCCAACTTCTATGAGAAACTTTACGCATTGCTGATTCCTTCAATTTTTATGGCTAAACATCGATCAAAGTTTTTTCAGCTTCTTGATTCTTGCTTAAAGTCACCACTTCTTCCTGCATATTTGGCTGCTGCTTTTGCTAAGAAATTGAGTAGGCTGTCAATTTCAGTTCCTCCTTCTGGAGCCTTGGTTATTATTGCGTTGATCCATAATCTTCTACGGAGACACCCTTCAATCAACTGTTTGGTGAATCGGCGAGGTGATGAAAAAACTGTGGAAGTTGATCCAGAAGCAGAGATAAGTACTCCTGAAGGTGCAGATGCCAATGGTACTGATGCAACAGATCAGTCGCTCAGAAAACCAGTTATTGATCCTTTTGATAATGAACAAAGTGATCCTAAGAAATCAAATGCCATGAGAAGTTCACTCTGGGAGATTGATACCCTCCGTCACCATTACTGCCCACATGTTGCAAGGTTTGTGGTGTCACTTGAGAATGATTTGACAGTCAGAGCGAAAACCACTGAAATTTCTGTTGGAGATTTCAGCTCGGGTTCATATGCGACAATATTTGGAGAAGAGATGAGACGGCGGGTAAAGCAGGCTCCAATATCATTCTACAGAACAACTCCCACCTCTTTATTCACGGAGCCTGAGACCGATTTTCTTGGGTGGACATTCAAGTGTGATGACACCAAGAGAAAGAACGATAGCACTCATGAGAATGGGGACATGCATCAGGAAAGTGATCGTAATTCAGGAAAACGACAAAGAGTTGAGTGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

442

Amino Acids

50.22

Weight (kDa)

8.59

Isoelectric Point (pI)

58.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 163 - 340 1.5e-46 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 65, 162, 1132
AclWI GGATC 5 cut(s) 217, 739, 818, 905, 929
AcsI RAATTY 3 cut(s) 84, 326, 1068
AcuI CTGAAG 2 cut(s) 243, 870
AfaI GTAC 4 cut(s) 380, 542, 844, 871
AfiI CCNNNNNNNGG 2 cut(s) 406, 719
AflIII ACRYGT 2 cut(s) 198, 1006
AgsI TTSAA 5 cut(s) 170, 182, 585, 774, 1222
AjuI GAANNNNNNNTTGG 2 cut(s) 1059, 1091
AluBI AGCT 3 cut(s) 78, 622, 1089
AluI AGCT 3 cut(s) 78, 622, 1089
Alw26I GTCTC 4 cut(s) 512, 757, 1120, 1190
AlwI GGATC 5 cut(s) 217, 739, 818, 905, 929
Ama87I CYCGRG 1 cut(s) 1090
ApeKI GCWGC 4 cut(s) 111, 296, 668, 671
ApoI RAATTY 3 cut(s) 84, 326, 1068
AseI ATTAAT 1 cut(s) 125
AsuHPI GGTGA 4 cut(s) 636, 799, 812, 983
AvaI CYCGRG 1 cut(s) 1090
BarI GAAGNNNNNNTAC 2 cut(s) 533, 565
BbsI GAAGAC 1 cut(s) 269
BbvI GCAGC 4 cut(s) 123, 308, 655, 658
BceAI ACGGC 1 cut(s) 1145
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BcoDI GTCTC 4 cut(s) 512, 757, 1120, 1190
BfaI CTAG 1 cut(s) 459
BfmI CTRYAG 2 cut(s) 294, 1159
BisI GCNGC 5 cut(s) 65, 112, 297, 669, 672
BlsI GCNGC 5 cut(s) 66, 113, 298, 670, 673
BmcAI AGTACT 1 cut(s) 844
BmeT110I CYCGRG 1 cut(s) 1090
BmiI GGNNCC 3 cut(s) 724, 1146, 1191
BmsI GCATC 4 cut(s) 51, 850, 865, 1285
BpiI GAAGAC 1 cut(s) 269
BpmI CTGGAG 1 cut(s) 741
BpuEI CTTGAG 1 cut(s) 1052
Bsa29I ATCGAT 1 cut(s) 604
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 1 cut(s) 726
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 719
Bse1I ACTGG 1 cut(s) 902
Bse3DI GCAATG 1 cut(s) 569
BseCI ATCGAT 1 cut(s) 604
BseDI CCNNGG 1 cut(s) 726
BseLI CCNNNNNNNGG 2 cut(s) 406, 719
BseMI GCAATG 1 cut(s) 569
BseMII CTCAG 4 cut(s) 225, 258, 907, 1185
BseNI ACTGG 1 cut(s) 902
BseXI GCAGC 4 cut(s) 123, 308, 655, 658
BsgI GTGCAG 1 cut(s) 876
BshVI ATCGAT 1 cut(s) 604
BsiHKCI CYCGRG 1 cut(s) 1090
BslFI GGGAC 1 cut(s) 1283
BslI CCNNNNNNNGG 2 cut(s) 406, 719
BsmAI GTCTC 4 cut(s) 512, 757, 1120, 1190
BsmBI CGTCTC 1 cut(s) 1120
BsmFI GGGAC 1 cut(s) 1283
BsmI GAATGC 1 cut(s) 229
Bso31I GGTCTC 1 cut(s) 1190
BsoBI CYCGRG 1 cut(s) 1090
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 9 cut(s) 209, 461, 605, 744, 823, 883, 910, 934, 1288
BspACI CCGC 3 cut(s) 65, 162, 1132
BspCNI CTCAG 4 cut(s) 226, 259, 906, 1186
BspDI ATCGAT 1 cut(s) 604
BspHI TCATGA 2 cut(s) 12, 1258
BspLI GGNNCC 3 cut(s) 724, 1146, 1191
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 5 cut(s) 217, 739, 818, 905, 929
BspTNI GGTCTC 1 cut(s) 1190
BsrDI GCAATG 1 cut(s) 569
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 1 cut(s) 902
BssECI CCNNGG 1 cut(s) 726
BssMI GATC 9 cut(s) 209, 461, 605, 744, 823, 883, 910, 934, 1288
BssT1I CCWWGG 1 cut(s) 726
Bst4CI ACNGT 3 cut(s) 782, 814, 1048
Bst6I CTCTTC 2 cut(s) 515, 1113
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 397, 1143
BstDEI CTNAG 8 cut(s) 89, 234, 267, 353, 681, 893, 939, 1194
BstEII GGTNACC 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 404
BstKTI GATC 9 cut(s) 212, 464, 608, 747, 826, 886, 913, 937, 1291
BstMAI GTCTC 4 cut(s) 512, 757, 1120, 1190
BstMBI GATC 9 cut(s) 209, 461, 605, 744, 823, 883, 910, 934, 1288
BstMWI GCNNNNNNNGC 4 cut(s) 293, 665, 677, 1138
BstNSI RCATGY 3 cut(s) 202, 1010, 1276
BstPI GGTNACC 1 cut(s) 357
BstSFI CTRYAG 2 cut(s) 294, 1159
BstV1I GCAGC 4 cut(s) 123, 308, 655, 658
BstV2I GAAGAC 1 cut(s) 269
Bsu15I ATCGAT 1 cut(s) 604
BsuTUI ATCGAT 1 cut(s) 604
BtsIMutI CAGTG 1 cut(s) 1062
Cac8I GCNNGC 2 cut(s) 397, 1143
CciI TCATGA 2 cut(s) 12, 1258
ClaI ATCGAT 1 cut(s) 604
CseI GACGC 1 cut(s) 478
Csp6I GTAC 4 cut(s) 379, 541, 843, 870
CspCI CAANNNNNGTGG 2 cut(s) 1002, 1037
CviAII CATG 9 cut(s) 13, 115, 199, 339, 493, 955, 1007, 1259, 1273
CviQI GTAC 4 cut(s) 379, 541, 843, 870
DdeI CTNAG 8 cut(s) 89, 234, 267, 353, 681, 893, 939, 1194
DpnI GATC 9 cut(s) 211, 463, 607, 746, 825, 885, 912, 936, 1290
DpnII GATC 9 cut(s) 209, 461, 605, 744, 823, 883, 910, 934, 1288
Eam1104I CTCTTC 2 cut(s) 515, 1113
EarI CTCTTC 2 cut(s) 515, 1113
Eco130I CCWWGG 1 cut(s) 726
Eco31I GGTCTC 1 cut(s) 1190
Eco57I CTGAAG 2 cut(s) 243, 870
Eco88I CYCGRG 1 cut(s) 1090
Eco91I GGTNACC 1 cut(s) 357
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 357
EcoRI GAATTC 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 726
EcoT22I ATGCAT 1 cut(s) 1278
ErhI CCWWGG 1 cut(s) 726
Esp3I CGTCTC 1 cut(s) 1120
FaeI CATG 9 cut(s) 16, 118, 202, 342, 496, 958, 1010, 1262, 1276
FaqI GGGAC 1 cut(s) 1283
FatI CATG 9 cut(s) 12, 114, 198, 338, 492, 954, 1006, 1258, 1272
FauI CCCGC 2 cut(s) 169, 1125
FauNDI CATATG 2 cut(s) 466, 1099
Fnu4HI GCNGC 5 cut(s) 65, 112, 297, 669, 672
Fsp4HI GCNGC 5 cut(s) 65, 112, 297, 669, 672
FspBI CTAG 1 cut(s) 459
GluI GCNGC 5 cut(s) 65, 112, 297, 669, 672
GsuI CTGGAG 1 cut(s) 741
HgaI GACGC 1 cut(s) 478
Hin1II CATG 9 cut(s) 16, 118, 202, 342, 496, 958, 1010, 1262, 1276
HinfI GANTC 5 cut(s) 45, 218, 577, 629, 790
HphI GGTGA 4 cut(s) 636, 799, 812, 983
Hpy166II GTNNAC 4 cut(s) 100, 379, 965, 1215
Hpy188I TCNGA 4 cut(s) 223, 235, 896, 1052
Hpy188III TCNNGA 9 cut(s) 13, 371, 626, 720, 827, 848, 1259, 1280, 1299
Hpy8I GTNNAC 4 cut(s) 100, 379, 965, 1215
HpyAV CCTTC 7 cut(s) 164, 218, 410, 591, 726, 780, 845
HpyCH4III ACNGT 3 cut(s) 782, 814, 1048
HpyCH4V TGCA 7 cut(s) 111, 296, 659, 857, 878, 1013, 1276
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 665, 677, 1138
HpyF3I CTNAG 8 cut(s) 89, 234, 267, 353, 681, 893, 939, 1194
Hsp92II CATG 9 cut(s) 16, 118, 202, 342, 496, 958, 1010, 1262, 1276
Kzo9I GATC 9 cut(s) 209, 461, 605, 744, 823, 883, 910, 934, 1288
LmnI GCTCC 4 cut(s) 118, 722, 1150, 1189
Lsp1109I GCAGC 4 cut(s) 123, 308, 655, 658
LweI GCATC 4 cut(s) 51, 850, 865, 1285
MaeI CTAG 1 cut(s) 459
MaeIII GTNAC 6 cut(s) 150, 357, 446, 642, 989, 1026
MalI GATC 9 cut(s) 211, 463, 607, 746, 825, 885, 912, 936, 1290
MboI GATC 9 cut(s) 209, 461, 605, 744, 823, 883, 910, 934, 1288
MboII GAAGA 5 cut(s) 274, 502, 644, 746, 1130
MmeI TCCRAC 1 cut(s) 1057
MnlI CCTC 6 cut(s) 378, 379, 723, 791, 995, 1186
Mph1103I ATGCAT 1 cut(s) 1278
MseI TTAA 6 cut(s) 26, 51, 125, 323, 455, 638
MslI CAYNNNNRTG 3 cut(s) 518, 1222, 1263
Mva1269I GAATGC 1 cut(s) 229
MwoI GCNNNNNNNGC 4 cut(s) 293, 665, 677, 1138
NdeI CATATG 2 cut(s) 466, 1099
NdeII GATC 9 cut(s) 209, 461, 605, 744, 823, 883, 910, 934, 1288
NlaIII CATG 9 cut(s) 16, 118, 202, 342, 496, 958, 1010, 1262, 1276
NlaIV GGNNCC 3 cut(s) 724, 1146, 1191
NmuCI GTSAC 5 cut(s) 150, 446, 642, 989, 1026
NsiI ATGCAT 1 cut(s) 1278
NspI RCATGY 3 cut(s) 202, 1010, 1276
PagI TCATGA 2 cut(s) 12, 1258
PciI ACATGT 2 cut(s) 198, 1006
PctI GAATGC 1 cut(s) 229
PfeI GAWTC 5 cut(s) 45, 218, 577, 629, 790
PkrI GCNGC 5 cut(s) 66, 113, 298, 670, 673
PscI ACATGT 2 cut(s) 198, 1006
PshBI ATTAAT 1 cut(s) 125
PspEI GGTNACC 1 cut(s) 357
PspN4I GGNNCC 3 cut(s) 724, 1146, 1191
PstI CTGCAG 1 cut(s) 298
RsaI GTAC 4 cut(s) 380, 542, 844, 871
RsaNI GTAC 4 cut(s) 379, 541, 843, 870
RseI CAYNNNNRTG 3 cut(s) 518, 1222, 1263
SaqAI TTAA 6 cut(s) 26, 51, 125, 323, 455, 638
SatI GCNGC 5 cut(s) 65, 112, 297, 669, 672
Sau3AI GATC 9 cut(s) 209, 461, 605, 744, 823, 883, 910, 934, 1288
ScaI AGTACT 1 cut(s) 844
SfaNI GCATC 4 cut(s) 51, 850, 865, 1285
SfcI CTRYAG 2 cut(s) 294, 1159
SmiMI CAYNNNNRTG 3 cut(s) 518, 1222, 1263
SmlI CTYRAG 1 cut(s) 1031
SmoI CTYRAG 1 cut(s) 1031
SsiI CCGC 3 cut(s) 65, 162, 1132
SspI AATATT 3 cut(s) 136, 304, 1110
SspMI CTAG 1 cut(s) 459
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 3 cut(s) 782, 814, 1048
TaqI TCGA 1 cut(s) 604
TaqII GACCGA 1 cut(s) 1214
TatI WGTACW 2 cut(s) 378, 842
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 5 cut(s) 45, 218, 577, 629, 790
Tru1I TTAA 6 cut(s) 26, 51, 125, 323, 455, 638
Tru9I TTAA 6 cut(s) 26, 51, 125, 323, 455, 638
TscAI CASTG 1 cut(s) 1069
TseFI GTSAC 5 cut(s) 150, 446, 642, 989, 1026
TseI GCWGC 4 cut(s) 111, 296, 668, 671
Tsp45I GTSAC 5 cut(s) 150, 446, 642, 989, 1026
TspDTI ATGAA 8 cut(s) 29, 128, 332, 392, 502, 819, 939, 1086
TspGWI ACGGA 3 cut(s) 775, 977, 1202
TspRI CASTG 1 cut(s) 1069
VspI ATTAAT 1 cut(s) 125
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 3 cut(s) 84, 326, 1068
XceI RCATGY 3 cut(s) 202, 1010, 1276
XspI CTAG 1 cut(s) 459
ZrmI AGTACT 1 cut(s) 844
Zsp2I ATGCAT 1 cut(s) 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.