Rroxscaffold_7G00187510

Nucleolar complex protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003349
Physical Location & Seq
Reverse (-)
26904967 .. 26911336
6370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187510.1

Sequence Viewer

Length: 1533 bp
ATGGAGTTTGTCAAGTTGGGAAATGGAGGAAAGTTTCACTCTGCTATATTCCATAGGTTTCTTCGTAACATTGTGTACTCTACAACGTCGCCGAATTTCTTGGTAGACTTGCTTGCATCAAAGTATTTCAAGTATATTGATGTCCGTTATTTTACCTACATTAGCTTGGAAAAACTTGCAAGAACTCTAGATGCAAAAGGCCTTTCTGAGGATAGAAATCTGAATGCAGATGGTAACAACGGGAGTCATCCTAGTGAAAGCATGGAGCAATTAATTCGCAATATTCATTACATTATGTCACATATCCCCGCGTTTGAAGGTTCAGTTGAAAAAACTGATTATGACATGTGGAGTGGATCAAGTGAATCTGAAGAGCATTCTGAGAGCCAGAAAGCAAAAGATAAAAAGAAGACTGAGAAAGATAACGATAAGGCGTTGTCTGCGGCCAATATTGTCAAAAAGATGAAATTAAAATTTACTAAAGCATGGTTATCATTTCTAAGGTTACCACTTCCTCTTGATGTGTACAAGGAGGTTCTTGCCAGCCTTCATCAGGCAGTCATTCCTTATATATCTAACCCCGTTGTGTTATGTGACTTCTTAACTAGATCATATGACATTGGTGGTGTTATCAGCGTCATGGCTCTTAGCAGTCTCTTCATCATTATGACAAAATATGGTTTAGAGTACCCCAACTTCTATGAGAAACTTTACGCACTGCTGATTCCTTCAATTTTTATGGCTAAACATCGATCAAAGTTTTTTCAGCTTCTTGATTCTTGCTTAAAGTCACCACTTCTTCCTGCATATTTGGCTGCTGCTTTTGCTAAGAAATTGAGTAGGCTGTCACTTTCAGTTCCTCCTTCTGGAGCCTTGGTTATTATTTGCCAAGGTGATGAAAAAACTGTGGAAGTTGATCCAGAAGCAGAGATAAGTACTCCTGAAGGTGCAGATGCCAATGGTACTGATGCAACAGATCAGTCGCTCAGAAAGCCAGTTATTGATCCTTTTGATAATGAACAAAGTGATCCTAAGAAATCAAATGCCATGAGAAGTTCACTCTGGGAGATTGATACCCTCCGTCACCATTACTGCCCACATGTTGCAAGGTTTGTGGTGTCACTTGAGAATGATTTGACAGTCAGAGCCAAAACCACTGAAATTTCTGTTGGAGATTTCAGCTCGGGTTCATATGCGACAATATTTGGAGAAGAGGTAAGGTCCGTAGTTCAAATCCCTCCTTCCTCACTCAAGATAAGGAAAACTGATCTTATTAGGATTTACTGTGGCCTACTTATTGCTTTGGATCTCAGCAGAATAGCTCTGATGAGACGGCGGGTAAAGCAGGCTCCAATATCATTCTACAGAACAACTCCCACCTCTTTATTCACGGAGTCTGAGACCGATTTTCTTGGGTGGACATTCAAGTGTGAGGACACCAAGAGAAAGAACGATAACACTCATGAGAATGGGGAAATGCATAAAGAAAGTGATCGTAATTCAGGAAAACGACAAAGAGTTGAGTGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

510

Amino Acids

57.66

Weight (kDa)

8.26

Isoelectric Point (pI)

48.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 212 - 371 9.6e-39 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 105
AccII CGCG 1 cut(s) 311
AciI CCGC 3 cut(s) 309, 443, 1336
AclWI GGATC 5 cut(s) 364, 911, 998, 1022, 1314
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 3 cut(s) 94, 473, 1161
AcuI CTGAAG 2 cut(s) 390, 963
AfaI GTAC 5 cut(s) 77, 527, 689, 937, 964
AfiI CCNNNNNNNGG 3 cut(s) 208, 553, 866
AflIII ACRYGT 2 cut(s) 345, 1099
AgsI TTSAA 6 cut(s) 130, 317, 329, 732, 1232, 1426
AjuI GAANNNNNNNTTGG 2 cut(s) 1152, 1184
AluBI AGCT 4 cut(s) 165, 769, 1182, 1322
AluI AGCT 4 cut(s) 165, 769, 1182, 1322
Alw26I GTCTC 3 cut(s) 659, 1324, 1394
AlwI GGATC 5 cut(s) 364, 911, 998, 1022, 1314
Ama87I CYCGRG 1 cut(s) 1183
AoxI GGCC 3 cut(s) 199, 444, 1288
ApeKI GCWGC 2 cut(s) 815, 818
ApoI RAATTY 3 cut(s) 94, 473, 1161
AseI ATTAAT 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 1221
AsuHPI GGTGA 3 cut(s) 783, 905, 1076
AvaI CYCGRG 1 cut(s) 1183
AvaII GGWCC 1 cut(s) 1221
BarI GAAGNNNNNNTAC 2 cut(s) 680, 712
BbsI GAAGAC 1 cut(s) 416
BbvI GCAGC 2 cut(s) 802, 805
BccI CCATC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 1349
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BcoDI GTCTC 3 cut(s) 659, 1324, 1394
BfaI CTAG 3 cut(s) 188, 252, 606
BfmI CTRYAG 1 cut(s) 1363
BisI GCNGC 3 cut(s) 444, 816, 819
BlsI GCNGC 3 cut(s) 445, 817, 820
BmcAI AGTACT 1 cut(s) 937
Bme18I GGWCC 1 cut(s) 1221
BmeT110I CYCGRG 1 cut(s) 1183
BmgT120I GGNCC 1 cut(s) 1221
BmiI GGNNCC 2 cut(s) 871, 1350
BmsI GCATC 4 cut(s) 125, 181, 943, 958
BpiI GAAGAC 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 1145, 1235
Bsa29I ATCGAT 1 cut(s) 751
BsaBI GATNNNNATC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 1394
BsaJI CCNNGG 2 cut(s) 873, 889
BsaXI ACNNNNNCTCC 4 cut(s) 861, 891, 1200, 1230
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 553, 866
Bse1I ACTGG 1 cut(s) 995
Bse8I GATNNNNATC 1 cut(s) 216
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 2 cut(s) 873, 889
BseGI GGATG 1 cut(s) 247
BseJI GATNNNNATC 1 cut(s) 216
BseLI CCNNNNNNNGG 3 cut(s) 208, 553, 866
BseMII CTCAG 6 cut(s) 198, 372, 405, 1000, 1324, 1389
BseNI ACTGG 1 cut(s) 995
BseXI GCAGC 2 cut(s) 802, 805
BsgI GTGCAG 1 cut(s) 969
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 3 cut(s) 201, 446, 1290
BshVI ATCGAT 1 cut(s) 751
BsiHKCI CYCGRG 1 cut(s) 1183
BslI CCNNNNNNNGG 3 cut(s) 208, 553, 866
BsmAI GTCTC 3 cut(s) 659, 1324, 1394
BsmBI CGTCTC 1 cut(s) 1324
BsmI GAATGC 2 cut(s) 229, 376
BsnI GGCC 3 cut(s) 201, 446, 1290
Bso31I GGTCTC 1 cut(s) 1394
BsoBI CYCGRG 1 cut(s) 1183
Bsp1407I TGTACA 1 cut(s) 525
BspACI CCGC 3 cut(s) 309, 443, 1336
BspANI GGCC 3 cut(s) 201, 446, 1290
BspCNI CTCAG 6 cut(s) 199, 373, 406, 999, 1323, 1390
BspDI ATCGAT 1 cut(s) 751
BspFNI CGCG 1 cut(s) 311
BspHI TCATGA 1 cut(s) 1462
BspLI GGNNCC 2 cut(s) 871, 1350
BspPI GGATC 5 cut(s) 364, 911, 998, 1022, 1314
BspQI GCTCTTC 1 cut(s) 366
BspTNI GGTCTC 1 cut(s) 1394
BsrGI TGTACA 1 cut(s) 525
BsrI ACTGG 1 cut(s) 995
BssECI CCNNGG 2 cut(s) 873, 889
BssT1I CCWWGG 2 cut(s) 873, 889
Bst4CI ACNGT 3 cut(s) 907, 1141, 1286
Bst6I CTCTTC 3 cut(s) 366, 662, 1206
BstAUI TGTACA 1 cut(s) 525
BstC8I GCNNGC 3 cut(s) 114, 544, 1347
BstEII GGTNACC 1 cut(s) 504
BstENI CCTNNNNNAGG 2 cut(s) 206, 551
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 311
BstMAI GTCTC 3 cut(s) 659, 1324, 1394
BstMWI GCNNNNNNNGC 5 cut(s) 440, 812, 824, 991, 1342
BstNSI RCATGY 2 cut(s) 349, 1103
BstPI GGTNACC 1 cut(s) 504
BstSFI CTRYAG 1 cut(s) 1363
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 2 cut(s) 802, 805
BstV2I GAAGAC 1 cut(s) 416
BstX2I RGATCY 1 cut(s) 1306
BstYI RGATCY 1 cut(s) 1306
Bsu15I ATCGAT 1 cut(s) 751
BsuRI GGCC 3 cut(s) 201, 446, 1290
BsuTUI ATCGAT 1 cut(s) 751
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 716
BtsIMutI CAGTG 2 cut(s) 716, 1155
Cac8I GCNNGC 3 cut(s) 114, 544, 1347
CciI TCATGA 1 cut(s) 1462
Cfr13I GGNCC 1 cut(s) 1221
ClaI ATCGAT 1 cut(s) 751
CseI GACGC 1 cut(s) 625
Csp6I GTAC 5 cut(s) 76, 526, 688, 936, 963
CspCI CAANNNNNGTGG 2 cut(s) 1095, 1130
CviAII CATG 7 cut(s) 262, 346, 486, 640, 1048, 1100, 1463
CviQI GTAC 5 cut(s) 76, 526, 688, 936, 963
EaeI YGGCCR 1 cut(s) 444
Eam1104I CTCTTC 3 cut(s) 366, 662, 1206
EarI CTCTTC 3 cut(s) 366, 662, 1206
Eco130I CCWWGG 2 cut(s) 873, 889
Eco147I AGGCCT 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 1394
Eco47I GGWCC 1 cut(s) 1221
Eco57I CTGAAG 2 cut(s) 390, 963
Eco88I CYCGRG 1 cut(s) 1183
Eco91I GGTNACC 1 cut(s) 504
EcoNI CCTNNNNNAGG 2 cut(s) 206, 551
EcoO65I GGTNACC 1 cut(s) 504
EcoT14I CCWWGG 2 cut(s) 873, 889
EcoT22I ATGCAT 1 cut(s) 1482
ErhI CCWWGG 2 cut(s) 873, 889
Esp3I CGTCTC 1 cut(s) 1324
FaeI CATG 7 cut(s) 265, 349, 489, 643, 1051, 1103, 1466
FatI CATG 7 cut(s) 261, 345, 485, 639, 1047, 1099, 1462
FauI CCCGC 2 cut(s) 316, 1329
FauNDI CATATG 2 cut(s) 613, 1192
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 444, 816, 819
FokI GGATG 1 cut(s) 234
Fsp4HI GCNGC 3 cut(s) 444, 816, 819
FspBI CTAG 3 cut(s) 188, 252, 606
GluI GCNGC 3 cut(s) 444, 816, 819
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 3 cut(s) 201, 446, 1290
HgaI GACGC 1 cut(s) 625
Hin1II CATG 7 cut(s) 265, 349, 489, 643, 1051, 1103, 1466
HinfI GANTC 5 cut(s) 244, 365, 724, 776, 1394
HphI GGTGA 3 cut(s) 783, 905, 1076
Hpy166II GTNNAC 5 cut(s) 76, 106, 526, 1058, 1419
Hpy188I TCNGA 8 cut(s) 208, 222, 370, 382, 989, 1145, 1326, 1399
Hpy188III TCNNGA 9 cut(s) 188, 518, 773, 867, 920, 941, 1252, 1463, 1503
Hpy8I GTNNAC 5 cut(s) 76, 106, 526, 1058, 1419
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 6 cut(s) 311, 557, 738, 873, 938, 1251
HpyCH4III ACNGT 3 cut(s) 907, 1141, 1286
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 9 cut(s) 116, 179, 194, 227, 806, 950, 971, 1106, 1480
HpyF10VI GCNNNNNNNGC 5 cut(s) 440, 812, 824, 991, 1342
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 7 cut(s) 265, 349, 489, 643, 1051, 1103, 1466
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 3 cut(s) 265, 869, 1354
Lsp1109I GCAGC 2 cut(s) 802, 805
LweI GCATC 4 cut(s) 125, 181, 943, 958
MaeI CTAG 3 cut(s) 188, 252, 606
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 9 cut(s) 65, 233, 297, 504, 593, 789, 846, 1082, 1119
MboII GAAGA 6 cut(s) 53, 383, 421, 649, 791, 1223
MflI RGATCY 1 cut(s) 1306
MluCI AATT 9 cut(s) 94, 269, 273, 467, 473, 732, 833, 1161, 1498
MlyI GAGTC 2 cut(s) 253, 1403
MmeI TCCRAC 1 cut(s) 1150
Mph1103I ATGCAT 1 cut(s) 1482
MseI TTAA 4 cut(s) 272, 470, 602, 785
MslI CAYNNNNRTG 4 cut(s) 252, 665, 1426, 1467
Mva1269I GAATGC 2 cut(s) 229, 376
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 5 cut(s) 440, 812, 824, 991, 1342
NdeI CATATG 2 cut(s) 613, 1192
NlaIII CATG 7 cut(s) 265, 349, 489, 643, 1051, 1103, 1466
NlaIV GGNNCC 2 cut(s) 871, 1350
NmuCI GTSAC 6 cut(s) 297, 593, 789, 846, 1082, 1119
NsiI ATGCAT 1 cut(s) 1482
NspI RCATGY 2 cut(s) 349, 1103
PagI TCATGA 1 cut(s) 1462
PceI AGGCCT 1 cut(s) 201
PciI ACATGT 2 cut(s) 345, 1099
PciSI GCTCTTC 1 cut(s) 366
PctI GAATGC 2 cut(s) 229, 376
PfeI GAWTC 3 cut(s) 365, 724, 776
PkrI GCNGC 3 cut(s) 445, 817, 820
PleI GAGTC 2 cut(s) 252, 1402
PpsI GAGTC 2 cut(s) 252, 1402
PscI ACATGT 2 cut(s) 345, 1099
PshBI ATTAAT 1 cut(s) 272
PspEI GGTNACC 1 cut(s) 504
PspN4I GGNNCC 2 cut(s) 871, 1350
PspPI GGNCC 1 cut(s) 1221
PsuI RGATCY 1 cut(s) 1306
RsaI GTAC 5 cut(s) 77, 527, 689, 937, 964
RsaNI GTAC 5 cut(s) 76, 526, 688, 936, 963
RseI CAYNNNNRTG 4 cut(s) 252, 665, 1426, 1467
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 4 cut(s) 272, 470, 602, 785
SatI GCNGC 3 cut(s) 444, 816, 819
Sau96I GGNCC 1 cut(s) 1221
ScaI AGTACT 1 cut(s) 937
SchI GAGTC 2 cut(s) 253, 1403
SfaNI GCATC 4 cut(s) 125, 181, 943, 958
SfcI CTRYAG 1 cut(s) 1363
SinI GGWCC 1 cut(s) 1221
SmiMI CAYNNNNRTG 4 cut(s) 252, 665, 1426, 1467
SmlI CTYRAG 2 cut(s) 1124, 1250
SmoI CTYRAG 2 cut(s) 1124, 1250
Sse9I AATT 9 cut(s) 94, 269, 273, 467, 473, 732, 833, 1161, 1498
SseBI AGGCCT 1 cut(s) 201
SsiI CCGC 3 cut(s) 309, 443, 1336
SspI AATATT 3 cut(s) 283, 451, 1203
SspMI CTAG 3 cut(s) 188, 252, 606
StuI AGGCCT 1 cut(s) 201
StyI CCWWGG 2 cut(s) 873, 889
TaaI ACNGT 3 cut(s) 907, 1141, 1286
TaiI ACGT 1 cut(s) 89
TaqI TCGA 1 cut(s) 751
TaqII GACCGA 1 cut(s) 1418
TasI AATT 9 cut(s) 94, 269, 273, 467, 473, 732, 833, 1161, 1498
TatI WGTACW 3 cut(s) 75, 525, 935
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 3 cut(s) 365, 724, 776
Tru1I TTAA 4 cut(s) 272, 470, 602, 785
Tru9I TTAA 4 cut(s) 272, 470, 602, 785
TscAI CASTG 2 cut(s) 723, 1162
TseFI GTSAC 6 cut(s) 297, 593, 789, 846, 1082, 1119
TseI GCWGC 2 cut(s) 815, 818
Tsp45I GTSAC 6 cut(s) 297, 593, 789, 846, 1082, 1119
TspDTI ATGAA 8 cut(s) 275, 479, 539, 649, 912, 1032, 1179, 1518
TspGWI ACGGA 4 cut(s) 134, 1070, 1213, 1406
TspRI CASTG 2 cut(s) 723, 1162
VpaK11BI GGWCC 1 cut(s) 1221
VspI ATTAAT 1 cut(s) 272
XagI CCTNNNNNAGG 2 cut(s) 206, 551
XapI RAATTY 3 cut(s) 94, 473, 1161
XbaI TCTAGA 1 cut(s) 187
XceI RCATGY 2 cut(s) 349, 1103
XmiI GTMKAC 1 cut(s) 105
XspI CTAG 3 cut(s) 188, 252, 606
ZrmI AGTACT 1 cut(s) 937
Zsp2I ATGCAT 1 cut(s) 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.