Rorug06G0049800

Nucleolar complex protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003349
Physical Location & Seq
Forward (+)
6625429 .. 6628469
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0049800.1

Sequence Viewer

Length: 510 bp
ATGCTATTGGTTCAATCTCGGAGGGACCCTTTTGGAGTTCAGTGTTGTAATCGTTTTGTGGCTTCGTTTTGGATTGATTCTTATGGCGGTGCACGGGTTTGGAAGAAATGCCAGCCCAGTGCTGTTAATATGTGCTCGTGGAGCTCAGCTGCTAGTTATGTGACACCTCCTGGTGGCGTTGCTAAGTTTTCTGGCAATCCCATTGTCGTGAAGGAGCAATTGTTGCAAAAGAGAGCTGGAACTGTAAGGGGTGCTACTATTGAAGAAATAGAAGCGGAGAAGTCTGTGATTGAAAAAGATGCTAAAGGCAGGATGGAAAAGACTATAGAAACGGTACGATCCAATTTCAATTCTATTCGGACAGGGAGAGCAAGCCCAGCCATGCTGGGCAAGATTGAGGTGATACGGTTGACCCTGCGTCAACTCACATCAGATAGACGGAAGGAGGCATCATCATCTGTGCCGGGGTTGGTGGTGGGGATAACAACGGAGGATCAGATAATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

169

Amino Acids

18.58

Weight (kDa)

9.61

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 87, 275
AclWI GGATC 2 cut(s) 333, 501
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 173
AgsI TTSAA 4 cut(s) 14, 263, 293, 349
AhdI GACNNNNNGTC 1 cut(s) 417
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 3 cut(s) 144, 149, 236
AluI AGCT 3 cut(s) 144, 149, 236
Alw21I GWGCWC 3 cut(s) 94, 137, 146
Alw44I GTGCAC 1 cut(s) 90
AlwI GGATC 2 cut(s) 333, 501
ApaLI GTGCAC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 25
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 1 cut(s) 412
AvaII GGWCC 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 94
BanII GRGCYC 1 cut(s) 146
BauI CACGAG 1 cut(s) 136
Bbv12I GWGCWC 3 cut(s) 94, 137, 146
BbvI GCAGC 1 cut(s) 136
BccI CCATC 1 cut(s) 307
BciT130I CCWGG 1 cut(s) 171
BcnI CCSGG 1 cut(s) 465
BfaI CTAG 1 cut(s) 153
BfmI CTRYAG 1 cut(s) 324
BisI GCNGC 1 cut(s) 150
BlpI GCTNAGC 1 cut(s) 145
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 2 cut(s) 171, 465
Bme18I GGWCC 1 cut(s) 25
BmeRI GACNNNNNGTC 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 2 cut(s) 26, 27
BmrFI CCNGG 2 cut(s) 171, 465
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 289, 458
BmuI ACTGGG 1 cut(s) 111
Bpu1102I GCTNAGC 1 cut(s) 145
BpuMI CCSGG 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 464
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 117
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 1 cut(s) 136
BseYI CCCAGC 2 cut(s) 376, 385
BsiHKAI GWGCWC 3 cut(s) 94, 137, 146
BsiSI CCGG 1 cut(s) 464
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 38
Bsp1286I GDGCHC 3 cut(s) 94, 137, 146
Bsp143I GATC 2 cut(s) 338, 493
Bsp1720I GCTNAGC 1 cut(s) 145
BspACI CCGC 2 cut(s) 87, 275
BspCNI CTCAG 1 cut(s) 158
BspLI GGNNCC 2 cut(s) 26, 27
BspPI GGATC 2 cut(s) 333, 501
BsrI ACTGG 1 cut(s) 117
BssECI CCNNGG 1 cut(s) 464
BssMI GATC 2 cut(s) 338, 493
BssSI CACGAG 1 cut(s) 136
Bst2BI CACGAG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 244, 334, 408
BstAPI GCANNNNNTGC 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 113, 373
BstDEI CTNAG 2 cut(s) 145, 183
BstF5I GGATG 1 cut(s) 318
BstKTI GATC 2 cut(s) 341, 496
BstMBI GATC 2 cut(s) 338, 493
BstMWI GCNNNNNNNGC 3 cut(s) 141, 223, 377
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 2 cut(s) 169, 463
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 318
BtsIMutI CAGTG 2 cut(s) 47, 124
Cac8I GCNNGC 2 cut(s) 113, 373
Cfr13I GGNCC 1 cut(s) 25
CseI GACGC 1 cut(s) 407
Csp6I GTAC 1 cut(s) 335
CviAII CATG 1 cut(s) 382
CviJI RGCY 7 cut(s) 62, 115, 144, 149, 236, 375, 380
CviKI_1 RGCY 7 cut(s) 62, 115, 144, 149, 236, 375, 380
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 2 cut(s) 145, 183
DpnI GATC 2 cut(s) 340, 495
DpnII GATC 2 cut(s) 338, 493
DriI GACNNNNNGTC 1 cut(s) 417
Eam1105I GACNNNNNGTC 1 cut(s) 417
Ecl136II GAGCTC 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 25
Eco53kI GAGCTC 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 146
FaeI CATG 1 cut(s) 385
FaiI YATR 5 cut(s) 84, 131, 159, 326, 383
FaqI GGGAC 1 cut(s) 38
FatI CATG 1 cut(s) 381
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 153
GluI GCNGC 1 cut(s) 150
GsaI CCCAGC 2 cut(s) 380, 389
HapII CCGG 1 cut(s) 464
HgaI GACGC 1 cut(s) 407
Hin1II CATG 1 cut(s) 385
HincII GTYRAC 2 cut(s) 411, 422
HindII GTYRAC 2 cut(s) 411, 422
HinfI GANTC 1 cut(s) 77
HpaII CCGG 1 cut(s) 464
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 3 cut(s) 92, 411, 422
Hpy188I TCNGA 4 cut(s) 21, 360, 433, 498
Hpy188III TCNNGA 1 cut(s) 208
Hpy8I GTNNAC 3 cut(s) 92, 411, 422
HpyAV CCTTC 2 cut(s) 205, 436
HpyCH4III ACNGT 3 cut(s) 244, 334, 408
HpyCH4V TGCA 2 cut(s) 92, 226
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 223, 377
HpyF3I CTNAG 2 cut(s) 145, 183
Hsp92II CATG 1 cut(s) 385
KflI GGGWCCC 1 cut(s) 25
Kzo9I GATC 2 cut(s) 338, 493
LmnI GCTCC 2 cut(s) 141, 214
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 2 cut(s) 289, 458
MaeI CTAG 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 160
MalI GATC 2 cut(s) 340, 495
MboI GATC 2 cut(s) 338, 493
MboII GAAGA 2 cut(s) 115, 275
MfeI CAATTG 1 cut(s) 218
MhlI GDGCHC 3 cut(s) 94, 137, 146
MluCI AATT 3 cut(s) 218, 343, 349
MnlI CCTC 5 cut(s) 15, 177, 391, 439, 484
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 2 cut(s) 171, 465
MunI CAATTG 1 cut(s) 218
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 141, 223, 377
NciI CCSGG 1 cut(s) 465
NdeII GATC 2 cut(s) 338, 493
NlaIII CATG 1 cut(s) 385
NlaIV GGNNCC 2 cut(s) 26, 27
NmuCI GTSAC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 1 cut(s) 151
PpuMI RGGWCCY 1 cut(s) 25
Psp124BI GAGCTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 25
Psp6I CCWGG 1 cut(s) 169
PspFI CCCAGC 2 cut(s) 376, 385
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 26, 27
PspPI GGNCC 1 cut(s) 25
PspPPI RGGWCCY 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 149
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 206
SacI GAGCTC 1 cut(s) 146
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 2 cut(s) 338, 493
Sau96I GGNCC 1 cut(s) 25
ScrFI CCNGG 2 cut(s) 171, 465
SduI GDGCHC 3 cut(s) 94, 137, 146
SetI ASST 5 cut(s) 146, 151, 169, 238, 402
SfaNI GCATC 2 cut(s) 289, 458
SfcI CTRYAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 3 cut(s) 218, 343, 349
SsiI CCGC 2 cut(s) 87, 275
SspMI CTAG 1 cut(s) 153
SstI GAGCTC 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 169, 463
TaaI ACNGT 3 cut(s) 244, 334, 408
TasI AATT 3 cut(s) 218, 343, 349
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 2 cut(s) 47, 124
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 2 cut(s) 454, 503
TspRI CASTG 2 cut(s) 47, 124
VneI GTGCAC 1 cut(s) 90
VpaK11BI GGWCC 1 cut(s) 25
XspI CTAG 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.