MD11G1014400.v1.1

Nucleolar complex protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003349
Physical Location & Seq
Forward (+)
1148686 .. 1151230
2545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1014400.v1.1.491

Sequence Viewer

Length: 795 bp
ATGAAGCTCTCAGGGTTGCTTTTCGATTTATTGAAAGAGGTGGTGTTGGACACGATAATGGAGTTTGTGATGTTCGGAAATGGTGGAAAGTTTCACTCTGCAATGTACCATAGGCTGCTTCGTAGTATTGTTTACACTACGTCACCAGTTGATTTGATGTTAGACTTGCTCTCATCCAAGTATTTCAAGTATATTGATGTCCGTTATTTTACCTACATTAGCTTGGAAAAGCTTTCCAAAGTTCTGGATGCAAATGGGATTTCTGGTACTTTCTGCTGTGTTCTTGCCACACTCCAGGCAGTCATTCCTTTTCTAGCTAACCCTGTCCTGTTATGCGACTTCTTAACTAGATCGTATGACATTGGTGGTGTTATCAGCGTCATGGCTCTTAGCAGTCTTTTCATCCTCATGACGCAACATGGTTTAGAGTACCCCAACTTCTATGAGAAACTTTATGCATTACTGGTTCCCTCTATTTTTCTGGCAAAACATCGATCGAAGTTTTTTCAGCTTGTAGATGCTTGCTTAAAATCGCCACTTCTTCCTGCATATTTGGCTGCGGCTTTTGCCAAGAAATTGAGTAGGCTATCAATTTCAGTTCCTCCATCTGGAGCGTTAGTTAATATTGCGCTGGTTCATAATCTTCTACGGAGACATCCTTCAATCAATTGCTTGGTGAATCGGGGAGGTGGTGATGAAACTGTGAAAGATGATCCAGAAGCAGAACAGAGGGTTGCTGATGGCGTGGATGGCACCGTAACTGGTTCGGCAGATAATTCAATAAAAAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

265

Amino Acids

29.2

Weight (kDa)

8.5

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 126 - 243 7.6e-30 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 752
AciI CCGC 1 cut(s) 560
AclWI GGATC 1 cut(s) 707
AfaI GTAC 3 cut(s) 107, 268, 431
AfiI CCNNNNNNNGG 1 cut(s) 608
AgsI TTSAA 4 cut(s) 34, 187, 663, 780
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 5 cut(s) 7, 222, 232, 317, 511
AluI AGCT 5 cut(s) 7, 222, 232, 317, 511
Alw26I GTCTC 1 cut(s) 646
AlwI GGATC 1 cut(s) 707
ApeKI GCWGC 2 cut(s) 115, 557
AspLEI GCGC 1 cut(s) 631
AsuHPI GGTGA 3 cut(s) 135, 688, 704
BanI GGYRCC 1 cut(s) 752
BarI GAAGNNNNNNTAC 2 cut(s) 422, 454
BbvI GCAGC 2 cut(s) 102, 544
BccI CCATC 3 cut(s) 613, 734, 743
BciT130I CCWGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 646
BfaI CTAG 2 cut(s) 314, 348
BisI GCNGC 3 cut(s) 116, 558, 561
BlsI GCNGC 3 cut(s) 117, 559, 562
Bme1390I CCNGG 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 468, 754
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 2 cut(s) 238, 508
BpmI CTGGAG 2 cut(s) 278, 630
Bsa29I ATCGAT 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 608
Bse1I ACTGG 3 cut(s) 146, 468, 766
Bse3DI GCAATG 1 cut(s) 108
BseBI CCWGG 1 cut(s) 296
BseCI ATCGAT 1 cut(s) 493
BseGI GGATG 5 cut(s) 173, 253, 402, 655, 754
BseLI CCNNNNNNNGG 1 cut(s) 608
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 3 cut(s) 146, 468, 766
BseXI GCAGC 2 cut(s) 102, 544
Bsh1285I CGRYCG 1 cut(s) 497
BshNI GGYRCC 1 cut(s) 752
BshVI ATCGAT 1 cut(s) 493
BsiEI CGRYCG 1 cut(s) 497
BslI CCNNNNNNNGG 1 cut(s) 608
BsmAI GTCTC 1 cut(s) 646
Bsp143I GATC 3 cut(s) 350, 494, 712
BspACI CCGC 1 cut(s) 560
BspCNI CTCAG 1 cut(s) 23
BspDI ATCGAT 1 cut(s) 493
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 2 cut(s) 468, 754
BspPI GGATC 1 cut(s) 707
BspT107I GGYRCC 1 cut(s) 752
BsrDI GCAATG 1 cut(s) 108
BsrI ACTGG 3 cut(s) 146, 468, 766
BssMI GATC 3 cut(s) 350, 494, 712
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 2 cut(s) 703, 757
BstC8I GCNNGC 1 cut(s) 523
BstDEI CTNAG 2 cut(s) 10, 389
BstF5I GGATG 5 cut(s) 173, 253, 402, 655, 754
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 3 cut(s) 353, 497, 715
BstMAI GTCTC 1 cut(s) 646
BstMBI GATC 3 cut(s) 350, 494, 712
BstMCI CGRYCG 1 cut(s) 497
BstMWI GCNNNNNNNGC 3 cut(s) 554, 566, 750
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 102, 544
BstXI CCANNNNNNTGG 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 493
BsuTUI ATCGAT 1 cut(s) 493
BtsCI GGATG 5 cut(s) 173, 253, 402, 655, 754
Cac8I GCNNGC 1 cut(s) 523
CciI TCATGA 1 cut(s) 408
CfoI GCGC 1 cut(s) 631
ClaI ATCGAT 1 cut(s) 493
CseI GACGC 2 cut(s) 367, 421
Csp6I GTAC 3 cut(s) 106, 267, 430
CviAII CATG 3 cut(s) 382, 409, 419
CviQI GTAC 3 cut(s) 106, 267, 430
DdeI CTNAG 2 cut(s) 10, 389
DpnI GATC 3 cut(s) 352, 496, 714
DpnII GATC 3 cut(s) 350, 494, 712
EcoRII CCWGG 1 cut(s) 294
EcoT22I ATGCAT 1 cut(s) 460
FaeI CATG 3 cut(s) 385, 412, 422
FatI CATG 3 cut(s) 381, 408, 418
Fnu4HI GCNGC 3 cut(s) 116, 558, 561
FokI GGATG 5 cut(s) 160, 260, 389, 642, 761
Fsp4HI GCNGC 3 cut(s) 116, 558, 561
FspBI CTAG 2 cut(s) 314, 348
GlaI GCGC 1 cut(s) 630
GluI GCNGC 3 cut(s) 116, 558, 561
GsuI CTGGAG 2 cut(s) 278, 630
HgaI GACGC 2 cut(s) 367, 421
HhaI GCGC 1 cut(s) 631
Hin1II CATG 3 cut(s) 385, 412, 422
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 1 cut(s) 679
HphI GGTGA 3 cut(s) 135, 688, 704
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 1 cut(s) 77
Hpy188III TCNNGA 4 cut(s) 245, 409, 609, 716
Hpy8I GTNNAC 1 cut(s) 133
HpyAV CCTTC 1 cut(s) 669
HpyCH4III ACNGT 2 cut(s) 703, 757
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 4 cut(s) 101, 251, 458, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 554, 566, 750
HpyF3I CTNAG 2 cut(s) 10, 389
HpySE526I ACGT 1 cut(s) 140
Hsp92II CATG 3 cut(s) 385, 412, 422
HspAI GCGC 1 cut(s) 629
Kzo9I GATC 3 cut(s) 350, 494, 712
LmnI GCTCC 1 cut(s) 611
Lsp1109I GCAGC 2 cut(s) 102, 544
LweI GCATC 2 cut(s) 238, 508
MaeI CTAG 2 cut(s) 314, 348
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 2 cut(s) 141, 757
MalI GATC 3 cut(s) 352, 496, 714
MboI GATC 3 cut(s) 350, 494, 712
MboII GAAGA 2 cut(s) 533, 635
MfeI CAATTG 1 cut(s) 667
MluCI AATT 4 cut(s) 575, 591, 667, 775
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 6 cut(s) 31, 416, 481, 612, 680, 723
Mph1103I ATGCAT 1 cut(s) 460
MseI TTAA 3 cut(s) 344, 527, 621
MslI CAYNNNNRTG 2 cut(s) 56, 407
MspR9I CCNGG 1 cut(s) 296
MunI CAATTG 1 cut(s) 667
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 3 cut(s) 554, 566, 750
NdeII GATC 3 cut(s) 350, 494, 712
NlaIII CATG 3 cut(s) 385, 412, 422
NlaIV GGNNCC 2 cut(s) 468, 754
NmuCI GTSAC 1 cut(s) 141
NsiI ATGCAT 1 cut(s) 460
PagI TCATGA 1 cut(s) 408
PfeI GAWTC 1 cut(s) 679
PkrI GCNGC 3 cut(s) 117, 559, 562
Ple19I CGATCG 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 2 cut(s) 468, 754
PvuI CGATCG 1 cut(s) 497
RsaI GTAC 3 cut(s) 107, 268, 431
RsaNI GTAC 3 cut(s) 106, 267, 430
RseI CAYNNNNRTG 2 cut(s) 56, 407
SaqAI TTAA 3 cut(s) 344, 527, 621
SatI GCNGC 3 cut(s) 116, 558, 561
Sau3AI GATC 3 cut(s) 350, 494, 712
ScrFI CCNGG 1 cut(s) 296
SetI ASST 9 cut(s) 9, 42, 143, 215, 224, 234, 319, 513, 691
SfaNI GCATC 2 cut(s) 238, 508
SmiMI CAYNNNNRTG 2 cut(s) 56, 407
Sse9I AATT 4 cut(s) 575, 591, 667, 775
SsiI CCGC 1 cut(s) 560
SspI AATATT 1 cut(s) 625
SspMI CTAG 2 cut(s) 314, 348
StyD4I CCNGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 703, 757
TaiI ACGT 1 cut(s) 143
TaqI TCGA 3 cut(s) 24, 493, 497
TasI AATT 4 cut(s) 575, 591, 667, 775
TauI GCSGC 1 cut(s) 563
TfiI GAWTC 1 cut(s) 679
Tru1I TTAA 3 cut(s) 344, 527, 621
Tru9I TTAA 3 cut(s) 344, 527, 621
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 2 cut(s) 115, 557
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 4 cut(s) 17, 391, 626, 711
TspGWI ACGGA 2 cut(s) 191, 664
XspI CTAG 2 cut(s) 314, 348
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.