RLG00000013018

Nucleolar complex protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003349
Physical Location & Seq
Reverse (-)
24905521 .. 24909579
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013018

Sequence Viewer

Length: 1356 bp
ATGGCCTCCATTCTCTCGAAGAAGCAAAAGCAGAAGCAGAAAACCACCAAGCTTTCCGACCTCCGAGACCTAGGCCACGACCTCCTCTCCTCCAGAACCCATATCAACAACCTCGCCAAGCTCCTCTCCTACGTCACCCCAACCTCGCCGCCGCAGTACGTCGTCGAATCCCTCCTCTCCATCCAAGCCTTCTTCACTCCCGTCTTGCCTGACCTCCCTTCCTCTTCTTCCAAGCCCAATCACTCCCAGGACGACGACCCCGAGTTCATATACCGCACCTGGCTCCGCTCCAAGTTCGACGAGCTCGTCGAGTCCCTCATCCAGCTCCTGCTTTCGCCGGAATCCGATGAAACTCTCAAGGAGGTGGTGTTGGATACACTGATGGAGTTTGTGAAGTTGGGAAATGGAGGAAAGTTTCACTCTGCTATATTCCATAGGTTTCTTCGTAACATTGTGTACTCTACAACGTCGCCGAATTTCCTGGTAGACTTGCTTGCATCAAAGTATTTCAAGTACATTGATGTCCGTTATTTTACCTACATTAGCTTGGAAAAACTTGCAAGAACTCTAGATGCAAAAGGCCTTTCTGAGGATAGAAATCTGAATGCAGATGGTAACAACGGGAGTCATCCTAGTGAAAGCATGGAGCAATTAATTCACAATATTCATTACATTATGTCACATATCCCCACTTTTGAAGGTTCAGTTGAAAAAACTGATTATGACATGTGGAGTGGATCAAGTGAATCTGAAGAGCATTCTGAGAGCCAGAAAGCAAAAGATAAAAAGAAGACTAAGAAAGATAACGATAAGGCATTGTCTGCAGCTAATATTGTCAAAAAGATGAAATTAAAATTTACTAAAGCATGGTTATCATTTCTAAGGTTACCACTTCCTCTTGATGTGTACAAGGAGGTTCTTGCCAGCCTTCATCAGGCAGTCATTCCTTATATATCTAACCCCGTTGTGTTATGTGACTTCTTAACTAGATCATATGACATTGGTGGTGTTATCAGCGTCATGGCTCTTAGCAGTCTCTTCATCATTATGACAAAATATGGTTTAGAGTACCCCAACTTCTATGAGAAACTTTACGCATTGCTGATTCCTTCAATTTTTATGGCTAAACATCGATCAAAGTTTTTTCAGCTTCTTGATTCTTGCTTAAAGTCACCACTTCTTCCTGCATATTTGGCTGCTGCTTTTGCTAAGAAATTGAGTAGGCTGTCACTTTCAGTTCCTCCTTCTGGAGCCTTGGTTATTATTGCGTTGATCCATAATCTTCTACGGAGACACCCTTCAATCAACTGTTTGGTGAATCGGGTAACTATAAAATCTTTATTTGGTCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

452

Amino Acids

51.04

Weight (kDa)

9.16

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 339 - 442 1.2e-28 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 288
AccI GTMKAC 1 cut(s) 486
AciI CCGC 4 cut(s) 149, 152, 274, 286
AclWI GGATC 2 cut(s) 745, 1267
AcsI RAATTY 2 cut(s) 475, 854
AcuI CTGAAG 1 cut(s) 771
AfaI GTAC 5 cut(s) 158, 458, 515, 908, 1070
AfiI CCNNNNNNNGG 3 cut(s) 589, 934, 1247
AflIII ACRYGT 1 cut(s) 726
AgsI TTSAA 5 cut(s) 511, 698, 710, 1113, 1302
AjnI CCWGG 3 cut(s) 246, 278, 480
AluBI AGCT 7 cut(s) 52, 121, 304, 325, 546, 827, 1150
AluI AGCT 7 cut(s) 52, 121, 304, 325, 546, 827, 1150
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 60, 1040, 1285
AlwI GGATC 2 cut(s) 745, 1267
AlwNI CAGNNNCTG 1 cut(s) 328
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 3 cut(s) 3, 73, 580
ApeKI GCWGC 3 cut(s) 824, 1196, 1199
ApoI RAATTY 2 cut(s) 475, 854
AseI ATTAAT 1 cut(s) 653
AspA2I CCTAGG 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 1346
AsuHPI GGTGA 3 cut(s) 127, 1164, 1327
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 1346
AvrII CCTAGG 1 cut(s) 70
BanII GRGCYC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 1061, 1093
BbsI GAAGAC 1 cut(s) 797
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 836, 1183, 1186
BccI CCATC 3 cut(s) 188, 376, 605
BciT130I CCWGG 3 cut(s) 248, 280, 482
BciVI GTATCC 1 cut(s) 367
BcoDI GTCTC 3 cut(s) 60, 1040, 1285
BfaI CTAG 4 cut(s) 71, 569, 633, 987
BfmI CTRYAG 1 cut(s) 822
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 5 cut(s) 149, 152, 825, 1197, 1200
BlnI CCTAGG 1 cut(s) 70
BlsI GCNGC 5 cut(s) 150, 153, 826, 1198, 1201
Bme1390I CCNGG 3 cut(s) 248, 280, 482
Bme18I GGWCC 1 cut(s) 1346
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 1346
BmiI GGNNCC 2 cut(s) 284, 1252
BmrFI CCNGG 3 cut(s) 248, 280, 482
BmsI GCATC 2 cut(s) 506, 562
BpiI GAAGAC 1 cut(s) 797
BpmI CTGGAG 2 cut(s) 76, 1269
BpuEI CTTGAG 1 cut(s) 341
Bsa29I ATCGAT 1 cut(s) 1132
BsaBI GATNNNNATC 1 cut(s) 597
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 3 cut(s) 70, 246, 1254
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 353, 383, 1242, 1272
Bsc4I CCNNNNNNNGG 3 cut(s) 589, 934, 1247
Bse3DI GCAATG 1 cut(s) 1097
Bse8I GATNNNNATC 1 cut(s) 597
BseBI CCWGG 3 cut(s) 248, 280, 482
BseCI ATCGAT 1 cut(s) 1132
BseDI CCNNGG 3 cut(s) 70, 246, 1254
BseGI GGATG 3 cut(s) 180, 318, 628
BseJI GATNNNNATC 1 cut(s) 597
BseLI CCNNNNNNNGG 3 cut(s) 589, 934, 1247
BseMI GCAATG 1 cut(s) 1097
BseMII CTCAG 2 cut(s) 579, 753
BseRI GAGGAG 4 cut(s) 74, 79, 113, 164
BseXI GCAGC 3 cut(s) 836, 1183, 1186
BshFI GGCC 3 cut(s) 5, 75, 582
BshVI ATCGAT 1 cut(s) 1132
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 3 cut(s) 589, 934, 1247
BsmAI GTCTC 3 cut(s) 60, 1040, 1285
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 2 cut(s) 610, 757
BsnI GGCC 3 cut(s) 5, 75, 582
Bso31I GGTCTC 1 cut(s) 60
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 306
Bsp1407I TGTACA 1 cut(s) 906
Bsp143I GATC 4 cut(s) 737, 989, 1133, 1272
BspACI CCGC 4 cut(s) 149, 152, 274, 286
BspANI GGCC 3 cut(s) 5, 75, 582
BspCNI CTCAG 2 cut(s) 580, 754
BspDI ATCGAT 1 cut(s) 1132
BspLI GGNNCC 2 cut(s) 284, 1252
BspMAI CTGCAG 1 cut(s) 826
BspPI GGATC 2 cut(s) 745, 1267
BspQI GCTCTTC 1 cut(s) 747
BspTNI GGTCTC 1 cut(s) 60
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 1097
BsrGI TGTACA 1 cut(s) 906
BssECI CCNNGG 3 cut(s) 70, 246, 1254
BssMI GATC 4 cut(s) 737, 989, 1133, 1272
BssT1I CCWWGG 2 cut(s) 70, 1254
Bst2UI CCWGG 3 cut(s) 248, 280, 482
Bst4CI ACNGT 1 cut(s) 1310
Bst6I CTCTTC 3 cut(s) 229, 747, 1043
BstAPI GCANNNNNTGC 1 cut(s) 821
BstAUI TGTACA 1 cut(s) 906
BstC8I GCNNGC 2 cut(s) 495, 925
BstDEI CTNAG 6 cut(s) 588, 762, 795, 881, 1028, 1209
BstEII GGTNACC 1 cut(s) 885
BstENI CCTNNNNNAGG 2 cut(s) 587, 932
BstF5I GGATG 3 cut(s) 180, 318, 628
BstKTI GATC 4 cut(s) 740, 992, 1136, 1275
BstMAI GTCTC 3 cut(s) 60, 1040, 1285
BstMBI GATC 4 cut(s) 737, 989, 1133, 1272
BstMWI GCNNNNNNNGC 3 cut(s) 821, 1193, 1205
BstNI CCWGG 3 cut(s) 248, 280, 482
BstNSI RCATGY 1 cut(s) 730
BstPI GGTNACC 1 cut(s) 885
BstSCI CCNGG 3 cut(s) 246, 278, 480
BstSFI CTRYAG 1 cut(s) 822
BstV1I GCAGC 3 cut(s) 836, 1183, 1186
BstV2I GAAGAC 1 cut(s) 797
Bsu15I ATCGAT 1 cut(s) 1132
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 3 cut(s) 5, 75, 582
BsuTUI ATCGAT 1 cut(s) 1132
BtsCI GGATG 3 cut(s) 180, 318, 628
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 2 cut(s) 495, 925
CaiI CAGNNNCTG 1 cut(s) 328
Cfr13I GGNCC 1 cut(s) 1346
ClaI ATCGAT 1 cut(s) 1132
CseI GACGC 1 cut(s) 1006
Csp6I GTAC 5 cut(s) 157, 457, 514, 907, 1069
CviAII CATG 4 cut(s) 643, 727, 867, 1021
CviQI GTAC 5 cut(s) 157, 457, 514, 907, 1069
DdeI CTNAG 6 cut(s) 588, 762, 795, 881, 1028, 1209
DpnI GATC 4 cut(s) 739, 991, 1135, 1274
DpnII GATC 4 cut(s) 737, 989, 1133, 1272
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
Eam1104I CTCTTC 3 cut(s) 229, 747, 1043
EarI CTCTTC 3 cut(s) 229, 747, 1043
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 2 cut(s) 70, 1254
Eco147I AGGCCT 1 cut(s) 582
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 1346
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 260
Eco91I GGTNACC 1 cut(s) 885
EcoICRI GAGCTC 1 cut(s) 304
EcoNI CCTNNNNNAGG 2 cut(s) 587, 932
EcoO65I GGTNACC 1 cut(s) 885
EcoRII CCWGG 3 cut(s) 246, 278, 480
EcoT14I CCWWGG 2 cut(s) 70, 1254
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 2 cut(s) 70, 1254
FaeI CATG 4 cut(s) 646, 730, 870, 1024
FaqI GGGAC 1 cut(s) 298
FatI CATG 4 cut(s) 642, 726, 866, 1020
FauNDI CATATG 1 cut(s) 994
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 5 cut(s) 149, 152, 825, 1197, 1200
FokI GGATG 3 cut(s) 167, 305, 615
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 5 cut(s) 149, 152, 825, 1197, 1200
FspBI CTAG 4 cut(s) 71, 569, 633, 987
GluI GCNGC 5 cut(s) 149, 152, 825, 1197, 1200
GsuI CTGGAG 2 cut(s) 76, 1269
HaeIII GGCC 3 cut(s) 5, 75, 582
HapII CCGG 1 cut(s) 338
HgaI GACGC 1 cut(s) 1006
Hin1II CATG 4 cut(s) 646, 730, 870, 1024
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 8 cut(s) 167, 311, 341, 625, 746, 1105, 1157, 1318
HpaII CCGG 1 cut(s) 338
HphI GGTGA 3 cut(s) 127, 1164, 1327
Hpy166II GTNNAC 3 cut(s) 457, 487, 907
Hpy188I TCNGA 7 cut(s) 58, 65, 346, 589, 603, 751, 763
Hpy188III TCNNGA 6 cut(s) 16, 93, 569, 899, 1154, 1248
Hpy8I GTNNAC 3 cut(s) 457, 487, 907
Hpy99I CGWCG 6 cut(s) 164, 167, 257, 302, 311, 472
HpyAV CCTTC 7 cut(s) 199, 228, 692, 938, 1119, 1254, 1308
HpyCH4III ACNGT 1 cut(s) 1310
HpyCH4IV ACGT 3 cut(s) 132, 159, 467
HpyCH4V TGCA 6 cut(s) 497, 560, 575, 608, 824, 1187
HpyF10VI GCNNNNNNNGC 3 cut(s) 821, 1193, 1205
HpyF3I CTNAG 6 cut(s) 588, 762, 795, 881, 1028, 1209
HpySE526I ACGT 3 cut(s) 132, 159, 467
Hsp92II CATG 4 cut(s) 646, 730, 870, 1024
Kzo9I GATC 4 cut(s) 737, 989, 1133, 1272
LguI GCTCTTC 1 cut(s) 747
LmnI GCTCC 6 cut(s) 126, 288, 293, 330, 646, 1250
Lsp1109I GCAGC 3 cut(s) 836, 1183, 1186
LweI GCATC 2 cut(s) 506, 562
MaeI CTAG 4 cut(s) 71, 569, 633, 987
MaeII ACGT 3 cut(s) 132, 159, 467
MaeIII GTNAC 9 cut(s) 133, 446, 614, 678, 885, 974, 1170, 1227, 1324
MalI GATC 4 cut(s) 739, 991, 1135, 1274
MbiI CCGCTC 1 cut(s) 288
MboI GATC 4 cut(s) 737, 989, 1133, 1272
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 7 cut(s) 475, 650, 654, 848, 854, 1113, 1214
MlyI GAGTC 2 cut(s) 320, 634
MmeI TCCRAC 2 cut(s) 81, 351
MseI TTAA 4 cut(s) 653, 851, 983, 1166
MslI CAYNNNNRTG 2 cut(s) 633, 1046
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 3 cut(s) 248, 280, 482
Mva1269I GAATGC 2 cut(s) 610, 757
MvaI CCWGG 3 cut(s) 248, 280, 482
MwoI GCNNNNNNNGC 3 cut(s) 821, 1193, 1205
NdeI CATATG 1 cut(s) 994
NdeII GATC 4 cut(s) 737, 989, 1133, 1272
NlaIII CATG 4 cut(s) 646, 730, 870, 1024
NlaIV GGNNCC 2 cut(s) 284, 1252
NmuCI GTSAC 5 cut(s) 133, 678, 974, 1170, 1227
NspI RCATGY 1 cut(s) 730
PceI AGGCCT 1 cut(s) 582
PciI ACATGT 1 cut(s) 726
PciSI GCTCTTC 1 cut(s) 747
PcsI WCGNNNNNNNCGW 3 cut(s) 258, 303, 306
PctI GAATGC 2 cut(s) 610, 757
PfeI GAWTC 6 cut(s) 167, 341, 746, 1105, 1157, 1318
PkrI GCNGC 5 cut(s) 150, 153, 826, 1198, 1201
PleI GAGTC 2 cut(s) 319, 633
PpsI GAGTC 2 cut(s) 319, 633
PscI ACATGT 1 cut(s) 726
PshBI ATTAAT 1 cut(s) 653
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 3 cut(s) 246, 278, 480
PspEI GGTNACC 1 cut(s) 885
PspGI CCWGG 3 cut(s) 246, 278, 480
PspN4I GGNNCC 2 cut(s) 284, 1252
PspPI GGNCC 1 cut(s) 1346
PstI CTGCAG 1 cut(s) 826
PstNI CAGNNNCTG 1 cut(s) 328
RsaI GTAC 5 cut(s) 158, 458, 515, 908, 1070
RsaNI GTAC 5 cut(s) 157, 457, 514, 907, 1069
RseI CAYNNNNRTG 2 cut(s) 633, 1046
SacI GAGCTC 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 747
SaqAI TTAA 4 cut(s) 653, 851, 983, 1166
SatI GCNGC 5 cut(s) 149, 152, 825, 1197, 1200
Sau3AI GATC 4 cut(s) 737, 989, 1133, 1272
Sau96I GGNCC 1 cut(s) 1346
SchI GAGTC 2 cut(s) 320, 634
ScrFI CCNGG 3 cut(s) 248, 280, 482
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 2 cut(s) 506, 562
SfcI CTRYAG 1 cut(s) 822
SinI GGWCC 1 cut(s) 1346
SmiMI CAYNNNNRTG 2 cut(s) 633, 1046
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 7 cut(s) 475, 650, 654, 848, 854, 1113, 1214
SseBI AGGCCT 1 cut(s) 582
SsiI CCGC 4 cut(s) 149, 152, 274, 286
SspI AATATT 2 cut(s) 664, 832
SspMI CTAG 4 cut(s) 71, 569, 633, 987
SstI GAGCTC 1 cut(s) 306
StuI AGGCCT 1 cut(s) 582
StyD4I CCNGG 3 cut(s) 246, 278, 480
StyI CCWWGG 2 cut(s) 70, 1254
TaaI ACNGT 1 cut(s) 1310
TaiI ACGT 3 cut(s) 135, 162, 470
TaqI TCGA 5 cut(s) 17, 165, 297, 309, 1132
TasI AATT 7 cut(s) 475, 650, 654, 848, 854, 1113, 1214
TatI WGTACW 3 cut(s) 456, 513, 906
TauI GCSGC 2 cut(s) 151, 154
TfiI GAWTC 6 cut(s) 167, 341, 746, 1105, 1157, 1318
Tru1I TTAA 4 cut(s) 653, 851, 983, 1166
Tru9I TTAA 4 cut(s) 653, 851, 983, 1166
TscAI CASTG 1 cut(s) 384
TseFI GTSAC 5 cut(s) 133, 678, 974, 1170, 1227
TseI GCWGC 3 cut(s) 824, 1196, 1199
Tsp45I GTSAC 5 cut(s) 133, 678, 974, 1170, 1227
TspDTI ATGAA 6 cut(s) 256, 363, 656, 860, 920, 1030
TspGWI ACGGA 2 cut(s) 515, 1303
TspRI CASTG 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 1346
VspI ATTAAT 1 cut(s) 653
XagI CCTNNNNNAGG 2 cut(s) 587, 932
XapI RAATTY 2 cut(s) 475, 854
XbaI TCTAGA 1 cut(s) 568
XceI RCATGY 1 cut(s) 730
XmaJI CCTAGG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 486
XspI CTAG 4 cut(s) 71, 569, 633, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.