AT2G19160

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004280
Physical Location & Seq
Forward (+)
8309533 .. 8314008
4476 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G19160.1

Sequence Viewer

Length: 1185 bp
ATGAAGGCAATTAAGGGATGGCGTCTAGGCAAAATTAATTATATGCAGTCTTTGCCTGGGGCTCGCCATCGTGCTCCTACAAGGAAGCCTATATGGATCATTGCAGTGCTTTCACTGATAGCAATGTTTGTAATTGGTGCTTACATGTTCCCTCATCACAGCAAAGCGGCATGTTATATGTTTTCATCTAAAGGGTGCAAGGGGCTAACTGATTGGCTTCCACCATCACTGAGGGAATATTCGGATGACGAGATTGCAGCTCGTGTAGTTATTAGTGAAATTTTGAGCTCCCCTCGTGTTATAAAAAAGAGTTCTAAAATTGCATTTATGTTCTTAACTCCTGGTACATTGCCTTTTGAAAAGCTATGGGACCTCTTTTTCCAGGGTCATGAGGGGAAATTCTCTGTTTATATACATGCATCAAAGGATACTCCGGTTCACACCAGTCGTTACTTTCTTAACCGTGAGATTCGAAGTGACGAGGTGGTCTGGGGTAGAATATCAATGATTGACGCTGAAAGACGTTTGTTGACCAATGCTCTTAGAGATCCTGAAAATCAGCAATTTGTATTACTCTCTGATAGTTGTGTGCCACTTCGAAGTTTTGAATACATGTACAACTATATGATGCACAGCAATGTCAGCTATGTTGACTGCTTTGATGATCCTGGTCCACATGGAACGGGCAGGCATATGGACCACATGTTGCCGGAAATTCCAAGGGAAGATTTTCGAAAGGGTGCACAGTGGTTCTCTATGAAGCGTCAGCATGCTGTAGTAACTGTGGCAGATAATCTTTACTACTCTAAATTCCGGGACTACTGTGGGCCAGGTGTAGAGGGCAACAAGAACTGCATTGCGGATGAACACTATCTGCCAACATTCTTCTATATGCTTGATCCTACTGGCATTGCTAACTGGACTGTGACATATGTTGATTGGTCTGAGAGAAAGTGGCATCCAAGAAAATACATGCCTGAAGATATCACGCTCGAGTTAATTAAGAACATCTCGTCCATTGACGCAGTTTCACGCGTAACAAGTGAGAAAAACGGAGTAGTATCCTGGACGCATTGCATGTGGAATGGAATCAAAAGACCTTGCTATCTCTTTGGAAGGAAATTTCATGCTGATACTCTCGATAAACTGATGGAACTCTTCCCAAACTACACAAGCATCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

394

Amino Acids

45.75

Weight (kDa)

8.84

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 107 - 337 9.8e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 302
AasI GACNNNNNNGTC 1 cut(s) 485
AccII CGCG 1 cut(s) 1035
AciI CCGC 2 cut(s) 167, 860
AclWI GGATC 4 cut(s) 104, 542, 659, 893
AcsI RAATTY 5 cut(s) 279, 398, 714, 809, 1121
AcuI CTGAAG 1 cut(s) 999
AcyI GRCGYC 1 cut(s) 22
AfaI GTAC 2 cut(s) 346, 617
AflIII ACRYGT 4 cut(s) 144, 612, 702, 1033
AgsI TTSAA 2 cut(s) 359, 608
AjnI CCWGG 6 cut(s) 55, 340, 381, 667, 829, 1064
AloI GAACNNNNNNTCC 2 cut(s) 998, 1030
AluBI AGCT 4 cut(s) 260, 288, 364, 645
AluI AGCT 4 cut(s) 260, 288, 364, 645
Alw21I GWGCWC 3 cut(s) 76, 290, 745
Alw44I GTGCAC 1 cut(s) 741
AlwI GGATC 4 cut(s) 104, 542, 659, 893
Ama87I CYCGRG 1 cut(s) 992
AoxI GGCC 1 cut(s) 827
ApaLI GTGCAC 1 cut(s) 741
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 5 cut(s) 279, 398, 714, 809, 1121
AseI ATTAAT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 729
AspS9I GGNCC 4 cut(s) 370, 671, 697, 827
AsuC2I CCSGG 1 cut(s) 815
AsuII TTCGAA 3 cut(s) 472, 598, 733
AvaI CYCGRG 1 cut(s) 992
AvaII GGWCC 3 cut(s) 370, 671, 697
BaeGI GKGCMC 1 cut(s) 745
BaeI ACNNNNGTAYC 2 cut(s) 420, 453
BanII GRGCYC 2 cut(s) 64, 290
BauI CACGAG 2 cut(s) 261, 294
Bbv12I GWGCWC 3 cut(s) 76, 290, 745
BbvI GCAGC 1 cut(s) 269
BccI CCATC 4 cut(s) 12, 75, 232, 1144
BciT130I CCWGG 6 cut(s) 57, 342, 383, 669, 831, 1066
BciVI GTATCC 2 cut(s) 421, 1072
BcnI CCSGG 1 cut(s) 815
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 774
BfuI GTATCC 2 cut(s) 421, 1072
BisI GCNGC 2 cut(s) 168, 258
BlsI GCNGC 2 cut(s) 169, 259
Bme1390I CCNGG 7 cut(s) 57, 342, 383, 669, 815, 831, 1066
Bme18I GGWCC 3 cut(s) 370, 671, 697
BmeT110I CYCGRG 1 cut(s) 992
BmgT120I GGNCC 4 cut(s) 370, 671, 697, 827
BmiI GGNNCC 1 cut(s) 371
BmrFI CCNGG 7 cut(s) 57, 342, 383, 669, 815, 831, 1066
BmsI GCATC 3 cut(s) 428, 618, 967
BplI GAGNNNNNCTC 2 cut(s) 277, 309
Bpu14I TTCGAA 3 cut(s) 472, 598, 733
BpuMI CCSGG 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 22
BsaJI CCNNGG 3 cut(s) 56, 382, 719
BsaWI WCCGGW 1 cut(s) 433
Bse1I ACTGG 3 cut(s) 444, 910, 923
Bse3DI GCAATG 7 cut(s) 99, 129, 347, 643, 855, 909, 1072
BseBI CCWGG 6 cut(s) 57, 342, 383, 669, 831, 1066
BseDI CCNNGG 3 cut(s) 56, 382, 719
BseGI GGATG 4 cut(s) 23, 250, 868, 958
BseMI GCAATG 7 cut(s) 99, 129, 347, 643, 855, 909, 1072
BseMII CTCAG 2 cut(s) 221, 936
BseNI ACTGG 3 cut(s) 444, 910, 923
BseSI GKGCMC 1 cut(s) 745
BseXI GCAGC 1 cut(s) 269
Bsh1236I CGCG 1 cut(s) 1035
BshFI GGCC 1 cut(s) 829
BsiHKAI GWGCWC 3 cut(s) 76, 290, 745
BsiHKCI CYCGRG 1 cut(s) 992
BsiSI CCGG 3 cut(s) 434, 710, 814
BslFI GGGAC 2 cut(s) 383, 830
BsmFI GGGAC 2 cut(s) 383, 830
BsnI GGCC 1 cut(s) 829
BsoBI CYCGRG 1 cut(s) 992
Bsp119I TTCGAA 3 cut(s) 472, 598, 733
Bsp1286I GDGCHC 4 cut(s) 64, 76, 290, 745
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 4 cut(s) 96, 547, 664, 898
BspACI CCGC 2 cut(s) 167, 860
BspANI GGCC 1 cut(s) 829
BspCNI CTCAG 2 cut(s) 222, 937
BspFNI CGCG 1 cut(s) 1035
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 371
BspPI GGATC 4 cut(s) 104, 542, 659, 893
BspT104I TTCGAA 3 cut(s) 472, 598, 733
BsrDI GCAATG 7 cut(s) 99, 129, 347, 643, 855, 909, 1072
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 3 cut(s) 444, 910, 923
BssECI CCNNGG 3 cut(s) 56, 382, 719
BssMI GATC 4 cut(s) 96, 547, 664, 898
BssNI GRCGYC 1 cut(s) 22
BssSI CACGAG 2 cut(s) 261, 294
BssT1I CCWWGG 1 cut(s) 719
Bst2BI CACGAG 2 cut(s) 261, 294
Bst2UI CCWGG 6 cut(s) 57, 342, 383, 669, 831, 1066
Bst4CI ACNGT 5 cut(s) 464, 747, 784, 824, 925
Bst6I CTCTTC 1 cut(s) 1163
BstACI GRCGYC 1 cut(s) 22
BstAPI GCANNNNNTGC 1 cut(s) 52
BstAUI TGTACA 1 cut(s) 615
BstBI TTCGAA 3 cut(s) 472, 598, 733
BstC8I GCNNGC 3 cut(s) 64, 689, 771
BstDEI CTNAG 3 cut(s) 230, 542, 945
BstF5I GGATG 4 cut(s) 23, 250, 868, 958
BstFNI CGCG 1 cut(s) 1035
BstKTI GATC 4 cut(s) 99, 550, 667, 901
BstMBI GATC 4 cut(s) 96, 547, 664, 898
BstMWI GCNNNNNNNGC 2 cut(s) 52, 642
BstNI CCWGG 6 cut(s) 57, 342, 383, 669, 831, 1066
BstNSI RCATGY 8 cut(s) 148, 174, 419, 616, 706, 773, 976, 1081
BstSCI CCNGG 7 cut(s) 55, 340, 381, 667, 813, 829, 1064
BstSFI CTRYAG 1 cut(s) 774
BstSLI GKGCMC 1 cut(s) 745
BstUI CGCG 1 cut(s) 1035
BstV1I GCAGC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
BsuI GTATCC 2 cut(s) 421, 1072
BsuRI GGCC 1 cut(s) 829
BtsCI GGATG 4 cut(s) 23, 250, 868, 958
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 4 cut(s) 111, 113, 227, 752
Cac8I GCNNGC 3 cut(s) 64, 689, 771
CciI TCATGA 1 cut(s) 388
Cfr13I GGNCC 4 cut(s) 370, 671, 697, 827
CseI GACGC 5 cut(s) 11, 521, 752, 1031, 1078
Csp6I GTAC 2 cut(s) 345, 616
CviJI RGCY 9 cut(s) 62, 88, 205, 217, 260, 288, 364, 645, 829
CviKI_1 RGCY 9 cut(s) 62, 88, 205, 217, 260, 288, 364, 645, 829
CviQI GTAC 2 cut(s) 345, 616
DdeI CTNAG 3 cut(s) 230, 542, 945
DpnI GATC 4 cut(s) 98, 549, 666, 900
DpnII GATC 4 cut(s) 96, 547, 664, 898
DrdI GACNNNNNNGTC 1 cut(s) 485
DseDI GACNNNNNNGTC 1 cut(s) 485
Eam1104I CTCTTC 1 cut(s) 1163
EarI CTCTTC 1 cut(s) 1163
Ecl136II GAGCTC 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 719
Eco24I GRGCYC 2 cut(s) 64, 290
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 3 cut(s) 370, 671, 697
Eco53kI GAGCTC 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 999
Eco88I CYCGRG 1 cut(s) 992
EcoICRI GAGCTC 1 cut(s) 288
EcoO109I RGGNCCY 1 cut(s) 370
EcoRII CCWGG 6 cut(s) 55, 340, 381, 667, 829, 1064
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 1 cut(s) 719
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 2 cut(s) 64, 290
ErhI CCWWGG 1 cut(s) 719
FaqI GGGAC 2 cut(s) 383, 830
FauNDI CATATG 2 cut(s) 693, 931
Fnu4HI GCNGC 2 cut(s) 168, 258
FokI GGATG 4 cut(s) 30, 257, 875, 945
FriOI GRGCYC 2 cut(s) 64, 290
Fsp4HI GCNGC 2 cut(s) 168, 258
FspBI CTAG 1 cut(s) 26
GluI GCNGC 2 cut(s) 168, 258
HaeIII GGCC 1 cut(s) 829
HapII CCGG 3 cut(s) 434, 710, 814
HgaI GACGC 5 cut(s) 11, 521, 752, 1031, 1078
Hin1I GRCGYC 1 cut(s) 22
HincII GTYRAC 2 cut(s) 531, 652
HindII GTYRAC 2 cut(s) 531, 652
HinfI GANTC 2 cut(s) 469, 1089
HpaII CCGG 3 cut(s) 434, 710, 814
Hpy166II GTNNAC 5 cut(s) 439, 531, 652, 674, 743
Hpy188I TCNGA 3 cut(s) 244, 580, 946
Hpy188III TCNNGA 3 cut(s) 389, 551, 1139
Hpy8I GTNNAC 5 cut(s) 439, 531, 652, 674, 743
HpyAV CCTTC 1 cut(s) 1110
HpyCH4III ACNGT 5 cut(s) 464, 747, 784, 824, 925
HpyCH4IV ACGT 1 cut(s) 523
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 642
HpyF3I CTNAG 3 cut(s) 230, 542, 945
HpySE526I ACGT 1 cut(s) 523
Hsp92I GRCGYC 1 cut(s) 22
Kzo9I GATC 4 cut(s) 96, 547, 664, 898
LmnI GCTCC 2 cut(s) 79, 293
Lsp1109I GCAGC 1 cut(s) 269
LweI GCATC 3 cut(s) 428, 618, 967
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 5 cut(s) 449, 476, 778, 925, 1036
MalI GATC 4 cut(s) 98, 549, 666, 900
MboI GATC 4 cut(s) 96, 547, 664, 898
MboII GAAGA 4 cut(s) 737, 877, 992, 1150
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 4 cut(s) 64, 76, 290, 745
MluI ACGCGT 1 cut(s) 1033
MnlI CCTC 7 cut(s) 162, 225, 303, 383, 385, 475, 832
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 6 cut(s) 12, 36, 335, 459, 998, 1002
MslI CAYNNNNRTG 2 cut(s) 104, 636
MspI CCGG 3 cut(s) 434, 710, 814
MspR9I CCNGG 7 cut(s) 57, 342, 383, 669, 815, 831, 1066
MvaI CCWGG 6 cut(s) 57, 342, 383, 669, 831, 1066
MvnI CGCG 1 cut(s) 1035
MwoI GCNNNNNNNGC 2 cut(s) 52, 642
NciI CCSGG 1 cut(s) 815
NdeI CATATG 2 cut(s) 693, 931
NdeII GATC 4 cut(s) 96, 547, 664, 898
NlaIV GGNNCC 1 cut(s) 371
NmuCI GTSAC 2 cut(s) 476, 925
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 8 cut(s) 148, 174, 419, 616, 706, 773, 976, 1081
NspV TTCGAA 3 cut(s) 472, 598, 733
PacI TTAATTAA 1 cut(s) 1002
PaeI GCATGC 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 992
PagI TCATGA 1 cut(s) 388
PciI ACATGT 3 cut(s) 144, 612, 702
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 2 cut(s) 469, 1089
PfoI TCCNGGA 2 cut(s) 813, 1064
PkrI GCNGC 2 cut(s) 169, 259
PpuMI RGGWCCY 1 cut(s) 370
PscI ACATGT 3 cut(s) 144, 612, 702
PshBI ATTAAT 1 cut(s) 36
PsiI TTATAA 1 cut(s) 302
Psp124BI GAGCTC 1 cut(s) 290
Psp5II RGGWCCY 1 cut(s) 370
Psp6I CCWGG 6 cut(s) 55, 340, 381, 667, 829, 1064
PspGI CCWGG 6 cut(s) 55, 340, 381, 667, 829, 1064
PspN4I GGNNCC 1 cut(s) 371
PspPI GGNCC 4 cut(s) 370, 671, 697, 827
PspPPI RGGWCCY 1 cut(s) 370
PspXI VCTCGAGB 1 cut(s) 992
PsuI RGATCY 1 cut(s) 547
RsaI GTAC 2 cut(s) 346, 617
RsaNI GTAC 2 cut(s) 345, 616
RseI CAYNNNNRTG 2 cut(s) 104, 636
SacI GAGCTC 1 cut(s) 290
SaqAI TTAA 6 cut(s) 12, 36, 335, 459, 998, 1002
SatI GCNGC 2 cut(s) 168, 258
Sau3AI GATC 4 cut(s) 96, 547, 664, 898
Sau96I GGNCC 4 cut(s) 370, 671, 697, 827
ScrFI CCNGG 7 cut(s) 57, 342, 383, 669, 815, 831, 1066
SduI GDGCHC 4 cut(s) 64, 76, 290, 745
SetI ASST 9 cut(s) 262, 290, 366, 375, 486, 526, 647, 835, 1102
SfaNI GCATC 3 cut(s) 428, 618, 967
SfcI CTRYAG 1 cut(s) 774
Sfr274I CTCGAG 1 cut(s) 992
SfuI TTCGAA 3 cut(s) 472, 598, 733
SinI GGWCC 3 cut(s) 370, 671, 697
SlaI CTCGAG 1 cut(s) 992
SmiMI CAYNNNNRTG 2 cut(s) 104, 636
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
SphI GCATGC 1 cut(s) 773
SsiI CCGC 2 cut(s) 167, 860
SspI AATATT 1 cut(s) 239
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 1 cut(s) 290
StyD4I CCNGG 7 cut(s) 55, 340, 381, 667, 813, 829, 1064
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 5 cut(s) 464, 747, 784, 824, 925
TaiI ACGT 1 cut(s) 526
TaqI TCGA 5 cut(s) 472, 598, 733, 993, 1140
TatI WGTACW 1 cut(s) 615
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 469, 1089
Tru1I TTAA 6 cut(s) 12, 36, 335, 459, 998, 1002
Tru9I TTAA 6 cut(s) 12, 36, 335, 459, 998, 1002
TscAI CASTG 4 cut(s) 111, 120, 234, 752
TseFI GTSAC 2 cut(s) 476, 925
TseI GCWGC 1 cut(s) 257
Tsp45I GTSAC 2 cut(s) 476, 925
TspDTI ATGAA 5 cut(s) 17, 174, 773, 879, 1115
TspGWI ACGGA 1 cut(s) 1068
TspRI CASTG 4 cut(s) 111, 120, 234, 752
VneI GTGCAC 1 cut(s) 741
VpaK11BI GGWCC 3 cut(s) 370, 671, 697
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 5 cut(s) 279, 398, 714, 809, 1121
XceI RCATGY 8 cut(s) 148, 174, 419, 616, 706, 773, 976, 1081
XhoI CTCGAG 1 cut(s) 992
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.