MD13G1244200.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004280
Physical Location & Seq
Reverse (-)
25476921 .. 25482521
5601 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1244200.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGAAGACGAGTCATTGGCGATTAGGCATGGGTGACATGCAGATCTTGCCTGGGGCACGCCATCGCCCACCTTTGAAGAGGCCAGTGTGGATTATTGTCTTGGTTTCTTTGGTCAGCATCTTTGTGATTTGTGCTTTTCTCTATCCACCTCAAAGCAACGCTGCTTGTTACATATTTTCTTCCAGAGGTTGTAAGGCTATTTCAGATTGGCTTCCACCGGCTCCTGCAAGGCAATTTACTGACGATGAGATTGCATCCCGTGTTGTGATCAGAGATATTTTAAGCGCACCTCCTATTCAATCCAAGAATCCAAAAATTGCATTCATGTTCCTTACACCAAGTGCATTGCCTTTTGAGAAGCTGTGGGATAAGTTTCTCCATGGTCATGAAGGGAAGTTCTCTATCTATGTGCATGCTTCTAAGGAAAGGGCAATTCATTTGAGCCGTTACTTTTTTAATCGAGAGATTCGCAGTGACCCGGTGATTTGGGGAAAAATTTCTATGGTAGATGCAGAGAGAAGATTATTGGCAAATGCTCTTCAAGATCCTGATAACCAGCATTTTGTCTTACTTTCTGAAAGTTGTGTACCATTGTACACATTTGACCATATCTATGACTATCTGATGAATACAAATATAAGCTATGTTGACTGCTTTGAGGATCCTGGTCCACACGGAAATGGGAGGTATTCTGAACACATGTTACCTGAAATTGAGAAGAAAGACTTTAGAAAGGGTGCACAGTGGTTCTCTATGAAGCGTCGGCATGCTGTGATAGTAATGGCTGATAATCTCTACTACTCGAAATTCCGGGACTACTGTTTTGACGGGCGCAATTGCATTGCTGATGAACATTACTTGCCAACCTTTTTCAATATAATTGATCCTGGTGGCATTGCAAACTGGTCCGTAACACATGTTGATTGGTCTGAAAGAAAGTGGCATCCGAAGTTATATAAGTCTCAGGATATTACACATGAGCTTCTGAAGAATATTACGTCGATTGATGTGAGCGTGCATGTAACCAGTGATGCGAAGAAAGTAGTACAGAAATGGCCTTGCTTATGGAACGGTATACAACGACCGTGTTACTTATTTGCCAGGAAGTTCCATCCAGAAACTCTGAATGACTTGTTGCGCCTTTTTGCCAATTATACAACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

389

Amino Acids

45.14

Weight (kDa)

8.82

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 106 - 332 2.5e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1075
AclWI GGATC 4 cut(s) 539, 656, 669, 878
AcsI RAATTY 2 cut(s) 495, 806
AcuI CTGAAG 1 cut(s) 1007
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 3 cut(s) 588, 596, 1047
AflIII ACRYGT 2 cut(s) 699, 916
AgsI TTSAA 4 cut(s) 76, 299, 542, 874
AjnI CCWGG 4 cut(s) 49, 664, 886, 1100
AluBI AGCT 3 cut(s) 361, 642, 982
AluI AGCT 3 cut(s) 361, 642, 982
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 1 cut(s) 966
Alw44I GTGCAC 1 cut(s) 738
AlwI GGATC 4 cut(s) 539, 656, 669, 878
AoxI GGCC 2 cut(s) 80, 1055
ApaLI GTGCAC 1 cut(s) 738
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 2 cut(s) 495, 806
ArsI GACNNNNNNTTYG 2 cut(s) 806, 838
Asp700I GAANNNNTTC 1 cut(s) 496
AspLEI GCGC 3 cut(s) 287, 834, 1140
AspS9I GGNCC 2 cut(s) 668, 906
AsuC2I CCSGG 2 cut(s) 479, 812
AsuHPI GGTGA 2 cut(s) 44, 493
AvaII GGWCC 2 cut(s) 668, 906
BaeGI GKGCMC 2 cut(s) 58, 742
BamHI GGATCC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 1 cut(s) 148
BccI CCATC 2 cut(s) 69, 1119
BceAI ACGGC 1 cut(s) 429
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 4 cut(s) 51, 666, 888, 1102
BclI TGATCA 1 cut(s) 267
BcnI CCSGG 2 cut(s) 479, 812
BcoDI GTCTC 1 cut(s) 966
BglII AGATCT 1 cut(s) 42
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 6 cut(s) 51, 479, 666, 812, 888, 1102
Bme18I GGWCC 2 cut(s) 668, 906
BmgT120I GGNCC 2 cut(s) 668, 906
BmiI GGNNCC 2 cut(s) 222, 663
BmrFI CCNGG 6 cut(s) 51, 479, 666, 812, 888, 1102
BmsI GCATC 5 cut(s) 126, 263, 499, 952, 1021
BpiI GAAGAC 1 cut(s) 11
BpuMI CCSGG 2 cut(s) 479, 812
BsaJI CCNNGG 2 cut(s) 50, 379
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 3 cut(s) 83, 908, 1026
Bse3DI GCAATG 3 cut(s) 344, 840, 894
BseBI CCWGG 4 cut(s) 51, 666, 888, 1102
BseDI CCNNGG 2 cut(s) 50, 379
BseGI GGATG 3 cut(s) 254, 943, 1111
BseMI GCAATG 3 cut(s) 344, 840, 894
BseMII CTCAG 1 cut(s) 977
BseNI ACTGG 3 cut(s) 83, 908, 1026
BseSI GKGCMC 2 cut(s) 58, 742
BseXI GCAGC 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 1085
BshFI GGCC 2 cut(s) 82, 1057
BsiEI CGRYCG 1 cut(s) 1085
BsiHKAI GWGCWC 1 cut(s) 742
BsiSI CCGG 3 cut(s) 218, 479, 811
BslFI GGGAC 1 cut(s) 827
BsmAI GTCTC 1 cut(s) 966
BsmFI GGGAC 1 cut(s) 827
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 2 cut(s) 82, 1057
Bsp1286I GDGCHC 2 cut(s) 58, 742
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 5 cut(s) 42, 267, 544, 661, 883
Bsp19I CCATGG 1 cut(s) 379
BspANI GGCC 2 cut(s) 82, 1057
BspCNI CTCAG 1 cut(s) 976
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 222, 663
BspPI GGATC 4 cut(s) 539, 656, 669, 878
BspQI GCTCTTC 1 cut(s) 543
BsrDI GCAATG 3 cut(s) 344, 840, 894
BsrFI RCCGGY 1 cut(s) 217
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 3 cut(s) 83, 908, 1026
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 2 cut(s) 50, 379
BssMI GATC 5 cut(s) 42, 267, 544, 661, 883
BssNAI GTATAC 1 cut(s) 1076
BssT1I CCWWGG 1 cut(s) 379
Bst1107I GTATAC 1 cut(s) 1076
Bst2UI CCWGG 4 cut(s) 51, 666, 888, 1102
Bst4CI ACNGT 5 cut(s) 744, 821, 1073, 1086, 1162
Bst6I CTCTTC 2 cut(s) 71, 543
BstAPI GCANNNNNTGC 1 cut(s) 46
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 4 cut(s) 58, 414, 768, 1016
BstDEI CTNAG 2 cut(s) 420, 963
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 3 cut(s) 254, 943, 1111
BstHHI GCGC 3 cut(s) 287, 834, 1140
BstKTI GATC 5 cut(s) 45, 270, 547, 664, 886
BstMAI GTCTC 1 cut(s) 966
BstMBI GATC 5 cut(s) 42, 267, 544, 661, 883
BstMCI CGRYCG 1 cut(s) 1085
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 4 cut(s) 51, 666, 888, 1102
BstNSI RCATGY 6 cut(s) 40, 416, 703, 770, 920, 1022
BstSCI CCNGG 6 cut(s) 49, 477, 664, 810, 886, 1100
BstSLI GKGCMC 2 cut(s) 58, 742
BstV1I GCAGC 1 cut(s) 148
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 3 cut(s) 42, 544, 661
BstYI RGATCY 3 cut(s) 42, 544, 661
BstZ17I GTATAC 1 cut(s) 1076
BsuRI GGCC 2 cut(s) 82, 1057
BtgI CCRYGG 1 cut(s) 379
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 254, 943, 1111
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 4 cut(s) 90, 478, 749, 1033
Cac8I GCNNGC 4 cut(s) 58, 414, 768, 1016
CciI TCATGA 1 cut(s) 385
CfoI GCGC 3 cut(s) 287, 834, 1140
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 668, 906
CseI GACGC 1 cut(s) 749
Csp6I GTAC 3 cut(s) 587, 595, 1046
CviQI GTAC 3 cut(s) 587, 595, 1046
DdeI CTNAG 2 cut(s) 420, 963
DpnI GATC 5 cut(s) 44, 269, 546, 663, 885
DpnII GATC 5 cut(s) 42, 267, 544, 661, 883
DraIII CACNNNGTG 1 cut(s) 341
Eam1104I CTCTTC 2 cut(s) 71, 543
EarI CTCTTC 2 cut(s) 71, 543
Eco130I CCWWGG 1 cut(s) 379
Eco47I GGWCC 2 cut(s) 668, 906
Eco57I CTGAAG 1 cut(s) 1007
EcoRII CCWGG 4 cut(s) 49, 664, 886, 1100
EcoT14I CCWWGG 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 379
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 1 cut(s) 827
FbaI TGATCA 1 cut(s) 267
FblI GTMKAC 1 cut(s) 1075
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 3 cut(s) 241, 930, 1098
Fsp4HI GCNGC 1 cut(s) 162
GlaI GCGC 3 cut(s) 286, 833, 1139
GluI GCNGC 1 cut(s) 162
HaeIII GGCC 2 cut(s) 82, 1057
HapII CCGG 3 cut(s) 218, 479, 811
HgaI GACGC 1 cut(s) 749
HhaI GCGC 3 cut(s) 287, 834, 1140
Hin6I GCGC 3 cut(s) 285, 832, 1138
HinP1I GCGC 3 cut(s) 285, 832, 1138
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 3 cut(s) 10, 307, 466
HpaII CCGG 3 cut(s) 218, 479, 811
HphI GGTGA 2 cut(s) 44, 493
Hpy166II GTNNAC 6 cut(s) 587, 597, 649, 671, 740, 1076
Hpy188I TCNGA 9 cut(s) 205, 272, 577, 624, 694, 931, 948, 987, 1125
Hpy188III TCNNGA 7 cut(s) 183, 386, 461, 542, 548, 965, 1115
Hpy8I GTNNAC 6 cut(s) 587, 597, 649, 671, 740, 1076
Hpy99I CGWCG 2 cut(s) 765, 1003
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 5 cut(s) 744, 821, 1073, 1086, 1162
HpyCH4IV ACGT 1 cut(s) 998
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 2 cut(s) 420, 963
HpySE526I ACGT 1 cut(s) 998
HspAI GCGC 3 cut(s) 285, 832, 1138
Ksp22I TGATCA 1 cut(s) 267
Kzo9I GATC 5 cut(s) 42, 267, 544, 661, 883
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 1 cut(s) 226
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 5 cut(s) 126, 263, 499, 952, 1021
MaeII ACGT 1 cut(s) 998
MaeIII GTNAC 8 cut(s) 32, 167, 446, 473, 702, 910, 1021, 1088
MalI GATC 5 cut(s) 44, 269, 546, 663, 885
MboI GATC 5 cut(s) 42, 267, 544, 661, 883
MboII GAAGA 8 cut(s) 16, 88, 171, 530, 531, 730, 1000, 1048
MfeI CAATTG 1 cut(s) 835
MflI RGATCY 3 cut(s) 42, 544, 661
MhlI GDGCHC 2 cut(s) 58, 742
MluCI AATT 9 cut(s) 233, 315, 432, 495, 711, 806, 835, 879, 1150
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 6 cut(s) 72, 159, 179, 300, 652, 678
MroXI GAANNNNTTC 1 cut(s) 496
MseI TTAA 2 cut(s) 281, 456
MslI CAYNNNNRTG 4 cut(s) 122, 384, 612, 678
MspI CCGG 3 cut(s) 218, 479, 811
MspR9I CCNGG 6 cut(s) 51, 479, 666, 812, 888, 1102
MunI CAATTG 1 cut(s) 835
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 4 cut(s) 51, 666, 888, 1102
MwoI GCNNNNNNNGC 1 cut(s) 46
NciI CCSGG 2 cut(s) 479, 812
NcoI CCATGG 1 cut(s) 379
NdeII GATC 5 cut(s) 42, 267, 544, 661, 883
NlaIV GGNNCC 2 cut(s) 222, 663
NmuCI GTSAC 2 cut(s) 32, 473
NspI RCATGY 6 cut(s) 40, 416, 703, 770, 920, 1022
PaeI GCATGC 2 cut(s) 416, 770
PagI TCATGA 1 cut(s) 385
PciI ACATGT 2 cut(s) 699, 916
PciSI GCTCTTC 1 cut(s) 543
PctI GAATGC 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 496
PfeI GAWTC 2 cut(s) 307, 466
PfoI TCCNGGA 1 cut(s) 810
PkrI GCNGC 1 cut(s) 163
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
PscI ACATGT 2 cut(s) 699, 916
Psp6I CCWGG 4 cut(s) 49, 664, 886, 1100
PspGI CCWGG 4 cut(s) 49, 664, 886, 1100
PspN4I GGNNCC 2 cut(s) 222, 663
PspPI GGNCC 2 cut(s) 668, 906
PsrI GAACNNNNNNTAC 2 cut(s) 687, 719
PsuI RGATCY 3 cut(s) 42, 544, 661
RsaI GTAC 3 cut(s) 588, 596, 1047
RsaNI GTAC 3 cut(s) 587, 595, 1046
RseI CAYNNNNRTG 4 cut(s) 122, 384, 612, 678
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 2 cut(s) 281, 456
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 5 cut(s) 42, 267, 544, 661, 883
Sau96I GGNCC 2 cut(s) 668, 906
SchI GAGTC 1 cut(s) 19
ScrFI CCNGG 6 cut(s) 51, 479, 666, 812, 888, 1102
SduI GDGCHC 2 cut(s) 58, 742
SfaNI GCATC 5 cut(s) 126, 263, 499, 952, 1021
SinI GGWCC 2 cut(s) 668, 906
SmiMI CAYNNNNRTG 4 cut(s) 122, 384, 612, 678
SphI GCATGC 2 cut(s) 416, 770
Sse9I AATT 9 cut(s) 233, 315, 432, 495, 711, 806, 835, 879, 1150
SspI AATATT 1 cut(s) 994
StyD4I CCNGG 6 cut(s) 49, 477, 664, 810, 886, 1100
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 5 cut(s) 744, 821, 1073, 1086, 1162
TaiI ACGT 1 cut(s) 1001
TaqI TCGA 3 cut(s) 460, 803, 1001
TasI AATT 9 cut(s) 233, 315, 432, 495, 711, 806, 835, 879, 1150
TatI WGTACW 2 cut(s) 594, 1045
TfiI GAWTC 2 cut(s) 307, 466
Tru1I TTAA 2 cut(s) 281, 456
Tru9I TTAA 2 cut(s) 281, 456
TscAI CASTG 4 cut(s) 90, 478, 749, 1033
TseFI GTSAC 2 cut(s) 32, 473
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 2 cut(s) 32, 473
TspDTI ATGAA 7 cut(s) 17, 313, 402, 425, 641, 770, 864
TspGWI ACGGA 2 cut(s) 690, 898
TspRI CASTG 4 cut(s) 90, 478, 749, 1033
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 2 cut(s) 668, 906
XapI RAATTY 2 cut(s) 495, 806
XceI RCATGY 6 cut(s) 40, 416, 703, 770, 920, 1022
XmiI GTMKAC 1 cut(s) 1075
XmnI GAANNNNTTC 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.