RchiOBHm_Chr4g0399961

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004280
Physical Location & Seq
Reverse (-)
17401307 .. 17407391
6085 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37205

Sequence Viewer

Length: 1182 bp
ATGAAGACGGGTCAGCCGTGGCGAATAGGCATGGGTGACATTCCTATATTTTCTGGGGCTCGCCATCGCTCACCTTTGAAGAGGCCGTTGTGGATCATTGTCTTGGTGTCTTTGGTTAGCATCTTTGTGATCTGTGCTTTTCTCTATCAACCGCAAAGCAACGCCGCTTGTTACATATTTTCTTCAAGAGGTTGTAAGGCTATTTCCGATTGGCTTCCACCTGCTCCAGCAAGGGAATATAATGATGATGAGATTGCAGCCCGGGTAGTGATAAAAGAAATTTTGAGTGCACCTCCTGTTCAATCAAAAAACCCGAAAATAGCATTCATGTTCCTTACACCAAGTGCAGTGCCTTTTGAGAAGCTGTGGGATAAATTTTTCATCCGTGGTCATGAGGGAAAGTTCTCTGTTTATGTGCATGCATCTAAAGAAAAGGCAGTTCATTTGAGCCGTTACTTTGCTAATAGAGAGATACGCAGTGATCAGGTGATTTGGGGAAAAATTTCCATGGTAGATGCTGAGAGACGATTATTGGCAAATGCTCTTCAAGATCCTGATAACCAGCACTTTGTGCTACTTTCTGAAAGTTGTGTACCATTGTATACGTTTGACTATATCTATGACTATCTGATGAATACAAATATAAGCTATGTTGACTGCTTTGAGGATCCTGGCCCACATGGAAATGGCAGGTACTCAGAACACATGTTACCTGAAATTGAGAAGAAAGACTTTAGAAAGGGTGCACAGTGGTTCTCTGTTAAGCGTCAGCATGCTGTTATAATAATGGCTGATAGTCTTTACTACTCAAAATTCCGGGATTACTGCAAGCCAGGTTTGGAGGGGCGCAATTGCATTGCTGATGAACATTACTTGCCAACTTTTTTCAATATAGTCGATCCTGGTGGAATTGCAAACTGGTCTGTAACACATGTTGATTGGTCTGAAAGAAAGTGGCACCCAAAGTTATACAAGGCTCAGGATATTAGTTACGAACTCCTGAGAAATATTACGTCGATTGATGTGAGTGTCCATGTAACCAGTGATGAAAAGAGGGTAGTGCAAAGATGGCCTTGCTTATGGAATGGTATACAACGACCGTGTTACTTGTTCGCAAGGAAATTCCATCCAGAGAGTCTCAATAACTTGTTGCACCTTTTTGCCAATTATACAACAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

393

Amino Acids

45.57

Weight (kDa)

8.7

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 107 - 337 1.9e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
AarI CACCTGC 1 cut(s) 229
Acc36I ACCTGC 2 cut(s) 229, 681
AccB1I GGYRCC 1 cut(s) 957
AccI GTMKAC 2 cut(s) 602, 1090
AciI CCGC 2 cut(s) 152, 165
AclWI GGATC 5 cut(s) 101, 545, 662, 675, 893
AcsI RAATTY 5 cut(s) 279, 374, 501, 812, 1121
AdeI CACNNNGTG 2 cut(s) 344, 571
AfaI GTAC 2 cut(s) 594, 695
AflIII ACRYGT 2 cut(s) 705, 931
AgsI TTSAA 5 cut(s) 79, 186, 302, 548, 889
AjnI CCWGG 3 cut(s) 670, 832, 901
AluBI AGCT 2 cut(s) 364, 648
AluI AGCT 2 cut(s) 364, 648
Alw21I GWGCWC 2 cut(s) 292, 748
Alw26I GTCTC 2 cut(s) 517, 1142
Alw44I GTGCAC 2 cut(s) 288, 744
AlwI GGATC 5 cut(s) 101, 545, 662, 675, 893
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 3 cut(s) 83, 673, 1070
ApaLI GTGCAC 2 cut(s) 288, 744
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 5 cut(s) 279, 374, 501, 812, 1121
Asp700I GAANNNNTTC 1 cut(s) 502
AspLEI GCGC 1 cut(s) 849
AspS9I GGNCC 1 cut(s) 674
AsuC2I CCSGG 3 cut(s) 262, 263, 818
AsuHPI GGTGA 3 cut(s) 47, 63, 499
AvaI CYCGRG 1 cut(s) 261
BaeGI GKGCMC 2 cut(s) 292, 748
BamHI GGATCC 1 cut(s) 667
BanI GGYRCC 1 cut(s) 957
BanII GRGCYC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 292, 748
BbvI GCAGC 1 cut(s) 269
BccI CCATC 3 cut(s) 72, 1062, 1134
BceAI ACGGC 2 cut(s) 70, 435
BciT130I CCWGG 3 cut(s) 672, 834, 903
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 3 cut(s) 262, 263, 818
BcoDI GTCTC 2 cut(s) 517, 1142
BfuAI ACCTGC 2 cut(s) 229, 681
BisI GCNGC 2 cut(s) 165, 258
BlsI GCNGC 2 cut(s) 166, 259
Bme1390I CCNGG 6 cut(s) 262, 263, 672, 818, 834, 903
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 2 cut(s) 669, 959
BmrFI CCNGG 6 cut(s) 262, 263, 672, 818, 834, 903
BmsI GCATC 3 cut(s) 129, 431, 505
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 978
BpuMI CCSGG 3 cut(s) 262, 263, 818
BsaJI CCNNGG 4 cut(s) 17, 261, 385, 507
Bse1I ACTGG 2 cut(s) 923, 1041
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 3 cut(s) 672, 834, 903
BseDI CCNNGG 4 cut(s) 17, 261, 385, 507
BseGI GGATG 2 cut(s) 381, 1126
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 4 cut(s) 510, 711, 992, 992
BseNI ACTGG 2 cut(s) 923, 1041
BseSI GKGCMC 2 cut(s) 292, 748
BseXI GCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 366
Bsh1285I CGRYCG 1 cut(s) 1100
BshFI GGCC 3 cut(s) 85, 675, 1072
BshNI GGYRCC 1 cut(s) 957
BsiEI CGRYCG 1 cut(s) 1100
BsiHKAI GWGCWC 2 cut(s) 292, 748
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 262, 817
BsmAI GTCTC 2 cut(s) 517, 1142
BsmBI CGTCTC 1 cut(s) 517
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 3 cut(s) 85, 675, 1072
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 61, 292, 748
Bsp143I GATC 6 cut(s) 93, 129, 481, 550, 667, 898
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 2 cut(s) 152, 165
BspANI GGCC 3 cut(s) 85, 675, 1072
BspCNI CTCAG 4 cut(s) 511, 710, 991, 993
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 2 cut(s) 669, 959
BspMI ACCTGC 2 cut(s) 229, 681
BspPI GGATC 5 cut(s) 101, 545, 662, 675, 893
BspQI GCTCTTC 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 957
BsrDI GCAATG 1 cut(s) 855
BsrI ACTGG 2 cut(s) 923, 1041
BssECI CCNNGG 4 cut(s) 17, 261, 385, 507
BssMI GATC 6 cut(s) 93, 129, 481, 550, 667, 898
BssNAI GTATAC 2 cut(s) 603, 1091
BssT1I CCWWGG 1 cut(s) 507
Bst1107I GTATAC 2 cut(s) 603, 1091
Bst2UI CCWGG 3 cut(s) 672, 834, 903
Bst4CI ACNGT 2 cut(s) 750, 1101
Bst6I CTCTTC 2 cut(s) 74, 549
BstAPI GCANNNNNTGC 1 cut(s) 571
BstC8I GCNNGC 4 cut(s) 61, 420, 774, 830
BstDEI CTNAG 4 cut(s) 519, 697, 978, 1001
BstDSI CCRYGG 3 cut(s) 17, 385, 507
BstF5I GGATG 2 cut(s) 381, 1126
BstHHI GCGC 1 cut(s) 849
BstKTI GATC 6 cut(s) 96, 132, 484, 553, 670, 901
BstMAI GTCTC 2 cut(s) 517, 1142
BstMBI GATC 6 cut(s) 93, 129, 481, 550, 667, 898
BstMCI CGRYCG 1 cut(s) 1100
BstMWI GCNNNNNNNGC 2 cut(s) 571, 1069
BstNI CCWGG 3 cut(s) 672, 834, 903
BstNSI RCATGY 4 cut(s) 422, 709, 776, 935
BstSCI CCNGG 6 cut(s) 260, 261, 670, 816, 832, 901
BstSLI GKGCMC 2 cut(s) 292, 748
BstV1I GCAGC 1 cut(s) 269
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 550, 667
BstYI RGATCY 2 cut(s) 550, 667
BstZ17I GTATAC 2 cut(s) 603, 1091
BsuRI GGCC 3 cut(s) 85, 675, 1072
BtgI CCRYGG 3 cut(s) 17, 385, 507
BtgZI GCGATG 1 cut(s) 50
BtsCI GGATG 2 cut(s) 381, 1126
BtsI GCAGTG 2 cut(s) 354, 484
BtsIMutI CAGTG 4 cut(s) 354, 484, 755, 1048
BveI ACCTGC 2 cut(s) 229, 681
Cac8I GCNNGC 4 cut(s) 61, 420, 774, 830
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 849
Cfr13I GGNCC 1 cut(s) 674
Cfr9I CCCGGG 1 cut(s) 261
CseI GACGC 1 cut(s) 755
Csp6I GTAC 2 cut(s) 593, 694
CviQI GTAC 2 cut(s) 593, 694
DdeI CTNAG 4 cut(s) 519, 697, 978, 1001
DpnI GATC 6 cut(s) 95, 131, 483, 552, 669, 900
DpnII GATC 6 cut(s) 93, 129, 481, 550, 667, 898
DraIII CACNNNGTG 2 cut(s) 344, 571
Eam1104I CTCTTC 2 cut(s) 74, 549
EarI CTCTTC 2 cut(s) 74, 549
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 261
EcoRII CCWGG 3 cut(s) 670, 832, 901
EcoT14I CCWWGG 1 cut(s) 507
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 507
Esp3I CGTCTC 1 cut(s) 517
FalI AAGNNNNNCTT 4 cut(s) 716, 748, 1057, 1089
FbaI TGATCA 1 cut(s) 481
FblI GTMKAC 2 cut(s) 602, 1090
Fnu4HI GCNGC 2 cut(s) 165, 258
FokI GGATG 2 cut(s) 368, 1113
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 165, 258
GlaI GCGC 1 cut(s) 848
GluI GCNGC 2 cut(s) 165, 258
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 3 cut(s) 85, 675, 1072
HapII CCGG 2 cut(s) 262, 817
HgaI GACGC 1 cut(s) 755
HhaI GCGC 1 cut(s) 849
Hin6I GCGC 1 cut(s) 847
HinP1I GCGC 1 cut(s) 847
HincII GTYRAC 1 cut(s) 655
HindII GTYRAC 1 cut(s) 655
HinfI GANTC 1 cut(s) 1135
HpaII CCGG 2 cut(s) 262, 817
HphI GGTGA 3 cut(s) 47, 63, 499
Hpy166II GTNNAC 6 cut(s) 290, 593, 603, 655, 746, 1091
Hpy188I TCNGA 5 cut(s) 208, 583, 630, 700, 946
Hpy188III TCNNGA 7 cut(s) 186, 392, 548, 554, 980, 1000, 1130
Hpy8I GTNNAC 6 cut(s) 290, 593, 603, 655, 746, 1091
Hpy99I CGWCG 1 cut(s) 1018
HpyCH4III ACNGT 2 cut(s) 750, 1101
HpyCH4IV ACGT 2 cut(s) 605, 1013
HpyF10VI GCNNNNNNNGC 2 cut(s) 571, 1069
HpyF3I CTNAG 4 cut(s) 519, 697, 978, 1001
HpySE526I ACGT 2 cut(s) 605, 1013
HspAI GCGC 1 cut(s) 847
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 6 cut(s) 93, 129, 481, 550, 667, 898
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 1 cut(s) 269
LweI GCATC 3 cut(s) 129, 431, 505
MaeII ACGT 2 cut(s) 605, 1013
MaeIII GTNAC 8 cut(s) 35, 170, 452, 708, 925, 989, 1036, 1103
MalI GATC 6 cut(s) 95, 131, 483, 552, 669, 900
MboI GATC 6 cut(s) 93, 129, 481, 550, 667, 898
MboII GAAGA 5 cut(s) 16, 91, 174, 536, 736
MfeI CAATTG 1 cut(s) 850
MflI RGATCY 2 cut(s) 550, 667
MhlI GDGCHC 3 cut(s) 61, 292, 748
MlyI GAGTC 1 cut(s) 1144
MnlI CCTC 7 cut(s) 75, 182, 303, 388, 658, 835, 1047
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 502
MseI TTAA 1 cut(s) 762
MslI CAYNNNNRTG 2 cut(s) 125, 684
MspI CCGG 2 cut(s) 262, 817
MspR9I CCNGG 6 cut(s) 262, 263, 672, 818, 834, 903
MunI CAATTG 1 cut(s) 850
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 3 cut(s) 672, 834, 903
MwoI GCNNNNNNNGC 2 cut(s) 571, 1069
NciI CCSGG 3 cut(s) 262, 263, 818
NcoI CCATGG 1 cut(s) 507
NdeII GATC 6 cut(s) 93, 129, 481, 550, 667, 898
NlaIV GGNNCC 2 cut(s) 669, 959
NmuCI GTSAC 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 4 cut(s) 422, 709, 776, 935
PaeI GCATGC 2 cut(s) 422, 776
PagI TCATGA 1 cut(s) 391
PaqCI CACCTGC 1 cut(s) 229
PciI ACATGT 2 cut(s) 705, 931
PciSI GCTCTTC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 502
PfoI TCCNGGA 1 cut(s) 816
PkrI GCNGC 2 cut(s) 166, 259
PleI GAGTC 1 cut(s) 1143
PpsI GAGTC 1 cut(s) 1143
PscI ACATGT 2 cut(s) 705, 931
PsiI TTATAA 1 cut(s) 782
Psp6I CCWGG 3 cut(s) 670, 832, 901
PspGI CCWGG 3 cut(s) 670, 832, 901
PspN4I GGNNCC 2 cut(s) 669, 959
PspPI GGNCC 1 cut(s) 674
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
PsuI RGATCY 2 cut(s) 550, 667
RsaI GTAC 2 cut(s) 594, 695
RsaNI GTAC 2 cut(s) 593, 694
RseI CAYNNNNRTG 2 cut(s) 125, 684
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 1 cut(s) 762
SatI GCNGC 2 cut(s) 165, 258
Sau3AI GATC 6 cut(s) 93, 129, 481, 550, 667, 898
Sau96I GGNCC 1 cut(s) 674
SchI GAGTC 1 cut(s) 1144
ScrFI CCNGG 6 cut(s) 262, 263, 672, 818, 834, 903
SduI GDGCHC 3 cut(s) 61, 292, 748
SfaNI GCATC 3 cut(s) 129, 431, 505
SmaI CCCGGG 1 cut(s) 263
SmiMI CAYNNNNRTG 2 cut(s) 125, 684
SphI GCATGC 2 cut(s) 422, 776
SsiI CCGC 2 cut(s) 152, 165
SspI AATATT 1 cut(s) 1009
StyD4I CCNGG 6 cut(s) 260, 261, 670, 816, 832, 901
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 2 cut(s) 750, 1101
TaiI ACGT 2 cut(s) 608, 1016
TaqI TCGA 2 cut(s) 897, 1016
TauI GCSGC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 4 cut(s) 354, 484, 755, 1048
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 7 cut(s) 17, 316, 370, 431, 647, 879, 1062
TspGWI ACGGA 1 cut(s) 374
TspMI CCCGGG 1 cut(s) 261
TspRI CASTG 4 cut(s) 354, 484, 755, 1048
VneI GTGCAC 2 cut(s) 288, 744
XapI RAATTY 5 cut(s) 279, 374, 501, 812, 1121
XceI RCATGY 4 cut(s) 422, 709, 776, 935
XmaI CCCGGG 1 cut(s) 261
XmiI GTMKAC 2 cut(s) 602, 1090
XmnI GAANNNNTTC 1 cut(s) 502
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.