AT4G25870

Core-2 I-branching beta-1,6-N-acetylglucosaminyltransferase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004280
Physical Location & Seq
Reverse (-)
13149170 .. 13152279
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25870.1

Sequence Viewer

Length: 1170 bp
ATGGGTGAGACGCAGAAGATTTTGCAAGGGCCACGTCACCACACATCTCTGAAGAAGCCCTTATGGGTTGTTTTGACAGTCTCAGTGACCAGCATGCTTCTAATCTGTACTCACATGTATCCAAAACATGGCAAGAGCTCCTCGTGTCACGGCCTCTATAGCACCAGAGGCTGTGAGGATGCTCTCTCTAAGTGGCTACCTGTTCATGTCAGGAAATTCACTGATGAGGAGATAGCAGCTCGAGCAGTGGTTAGAGACATACTAAGAACGCCTCCCTTCATAACCAATAACTCCAAAATCGCTTTCTTGTTCTTGACTCCCGGTACTTTGCCATTTGAGAAGCTCTGGGATGAATTTTTCAAGGGTCATGAAGGGAAATTCTCGATATATATCCACCCGTCAAAGGAACGACCAGTTCACATCAGCCGTCACTTTTCTGATCGAGAGATTCACAGTGATGAGGTTACTTGGGGAAGGATTTCTATGGTTGATGCTGAGAAGCGGTTACTGGTTAGCGCTCTTGAAGATCCTGACAACCAACACTTTGTTCTTGTTTCAGAGAGTTGTATTCCGTTGCATACTTTTGACTACACTTATAGATATTTGTTGTACTCCAACGTTAGCTTCATTGAGAGTTTTGTGGATCCGGGTCCACATGGAACCGGTAGACATATGGAACACATGTTACCAGAAATCGCCAAGGAAGATTTTAGAAAGGGTGCTCAGTGGTTCACAATGAAGCGACAACACGCGATTATAGTGATGGCCGATGGTCTTTACTACTCAAAATTCCGCGAGTATTGTGGACCTGGAATAGAGGCCGACAAGAACTGCATTGCCGATGAACATTACTTGCCAACATTCTTCAATATGATTGATCCCATGGGGATCTCAAATTGGTCAGTGACGTTTGTTGATTGGTCTGAACGAAGGTGGCATCCAAAGACATACGGAGGAAACGAGATTTCATTAGAATTTATGAAAAATGTCACATCCGAGGACATGAGTGTACATGTCACCAGTGTGGGGAAGCATGGAGATGAGCTGCATTGGCCTTGTACATGGAACGGGATTAAACGGCCTTGCTATCTATTTGCAAGAAAGTTCCATCCTGATACTCTCGACACATTGGTCAACCTCTTCCCAAACTACACAAACACAGCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

389

Amino Acids

45.01

Weight (kDa)

6.76

Isoelectric Point (pI)

33.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 100 - 330 2.2e-76 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1130
AccB7I CCANNNNNTGG 1 cut(s) 128
AccI GTMKAC 1 cut(s) 667
AccII CGCG 2 cut(s) 752, 795
AciI CCGC 2 cut(s) 502, 793
AclI AACGTT 1 cut(s) 618
AclWI GGATC 5 cut(s) 521, 638, 651, 872, 896
AcoI YGGCCR 1 cut(s) 765
AcsI RAATTY 5 cut(s) 215, 353, 377, 788, 974
AcuI CTGAAG 1 cut(s) 71
AfaI GTAC 5 cut(s) 109, 325, 611, 1011, 1060
AfeI AGCGCT 1 cut(s) 517
AfiI CCNNNNNNNGG 3 cut(s) 128, 403, 1026
AflIII ACRYGT 3 cut(s) 114, 681, 1012
AgeI ACCGGT 1 cut(s) 662
AgsI TTSAA 3 cut(s) 361, 524, 868
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 1 cut(s) 808
AjuI GAANNNNNNNTTGG 6 cut(s) 287, 319, 531, 563, 608, 640
AleI CACNNNNGTG 1 cut(s) 1022
AluBI AGCT 6 cut(s) 138, 239, 343, 624, 1045, 1163
AluI AGCT 6 cut(s) 138, 239, 343, 624, 1045, 1163
Alw21I GWGCWC 2 cut(s) 140, 724
Alw26I GTCTC 3 cut(s) 2, 85, 249
AlwI GGATC 5 cut(s) 521, 638, 651, 872, 896
AlwNI CAGNNNCTG 1 cut(s) 171
Ama87I CYCGRG 1 cut(s) 240
Aor51HI AGCGCT 1 cut(s) 517
AoxI GGCC 6 cut(s) 29, 151, 765, 819, 1052, 1079
ApeKI GCWGC 2 cut(s) 236, 1045
ApoI RAATTY 5 cut(s) 215, 353, 377, 788, 974
AsiGI ACCGGT 1 cut(s) 662
Asp700I GAANNNNTTC 1 cut(s) 478
AspLEI GCGC 1 cut(s) 518
AspS9I GGNCC 3 cut(s) 29, 650, 806
AsuC2I CCSGG 2 cut(s) 321, 648
AsuHPI GGTGA 3 cut(s) 17, 29, 1009
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 2 cut(s) 650, 806
BamHI GGATCC 1 cut(s) 643
BanII GRGCYC 1 cut(s) 140
BauI CACGAG 1 cut(s) 142
Bbv12I GWGCWC 2 cut(s) 140, 724
BbvI GCAGC 2 cut(s) 248, 1032
BccI CCATC 3 cut(s) 757, 764, 1116
BceAI ACGGC 3 cut(s) 166, 411, 1094
BciT130I CCWGG 1 cut(s) 810
BciVI GTATCC 1 cut(s) 129
BcnI CCSGG 2 cut(s) 321, 648
BcoDI GTCTC 3 cut(s) 2, 85, 249
BfmI CTRYAG 1 cut(s) 157
BfoI RGCGCY 1 cut(s) 519
BfuI GTATCC 1 cut(s) 129
BisI GCNGC 2 cut(s) 237, 1046
BlsI GCNGC 2 cut(s) 238, 1047
Bme1390I CCNGG 3 cut(s) 321, 648, 810
Bme18I GGWCC 2 cut(s) 650, 806
BmeT110I CYCGRG 1 cut(s) 240
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 3 cut(s) 29, 650, 806
BmiI GGNNCC 3 cut(s) 645, 651, 661
BmrFI CCNGG 3 cut(s) 321, 648, 810
BmsI GCATC 3 cut(s) 169, 481, 946
BpuMI CCSGG 2 cut(s) 321, 648
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 3 cut(s) 699, 882, 996
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 403, 1026
Bse118I RCCGGY 1 cut(s) 662
Bse1I ACTGG 3 cut(s) 413, 513, 1020
Bse3DI GCAATG 1 cut(s) 834
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 1 cut(s) 810
BseDI CCNNGG 3 cut(s) 699, 882, 996
BseGI GGATG 5 cut(s) 184, 355, 937, 992, 1108
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 3 cut(s) 128, 403, 1026
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 3 cut(s) 96, 486, 737
BseNI ACTGG 3 cut(s) 413, 513, 1020
BseRI GAGGAG 2 cut(s) 130, 242
BseXI GCAGC 2 cut(s) 248, 1032
Bsh1236I CGCG 2 cut(s) 752, 795
BshFI GGCC 6 cut(s) 31, 153, 767, 821, 1054, 1081
BshTI ACCGGT 1 cut(s) 662
BsiHKAI GWGCWC 2 cut(s) 140, 724
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 3 cut(s) 321, 647, 663
BslI CCNNNNNNNGG 3 cut(s) 128, 403, 1026
BsmAI GTCTC 3 cut(s) 2, 85, 249
BsmBI CGTCTC 1 cut(s) 2
BsnI GGCC 6 cut(s) 31, 153, 767, 821, 1054, 1081
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 2 cut(s) 140, 724
Bsp1407I TGTACA 2 cut(s) 1009, 1058
Bsp143I GATC 5 cut(s) 439, 526, 643, 877, 888
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 2 cut(s) 502, 793
BspANI GGCC 6 cut(s) 31, 153, 767, 821, 1054, 1081
BspCNI CTCAG 3 cut(s) 95, 487, 736
BspFNI CGCG 2 cut(s) 752, 795
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 645, 651, 661
BspPI GGATC 5 cut(s) 521, 638, 651, 872, 896
BsrDI GCAATG 1 cut(s) 834
BsrFI RCCGGY 1 cut(s) 662
BsrGI TGTACA 2 cut(s) 1009, 1058
BsrI ACTGG 3 cut(s) 413, 513, 1020
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 3 cut(s) 699, 882, 996
BssMI GATC 5 cut(s) 439, 526, 643, 877, 888
BssSI CACGAG 1 cut(s) 142
BssT1I CCWWGG 2 cut(s) 699, 882
Bst2BI CACGAG 1 cut(s) 142
Bst2UI CCWGG 1 cut(s) 810
Bst4CI ACNGT 2 cut(s) 79, 455
Bst6I CTCTTC 1 cut(s) 1145
BstAUI TGTACA 2 cut(s) 1009, 1058
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 5 cut(s) 82, 189, 263, 495, 723
BstDSI CCRYGG 1 cut(s) 882
BstF5I GGATG 5 cut(s) 184, 355, 937, 992, 1108
BstFNI CGCG 2 cut(s) 752, 795
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 5 cut(s) 442, 529, 646, 880, 891
BstMAI GTCTC 3 cut(s) 2, 85, 249
BstMBI GATC 5 cut(s) 439, 526, 643, 877, 888
BstMWI GCNNNNNNNGC 4 cut(s) 159, 168, 242, 1051
BstNI CCWGG 1 cut(s) 810
BstNSI RCATGY 4 cut(s) 97, 118, 685, 1016
BstSCI CCNGG 3 cut(s) 319, 646, 808
BstSFI CTRYAG 1 cut(s) 157
BstUI CGCG 2 cut(s) 752, 795
BstV1I GCAGC 2 cut(s) 248, 1032
BstX2I RGATCY 3 cut(s) 526, 643, 888
BstYI RGATCY 3 cut(s) 526, 643, 888
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 6 cut(s) 31, 153, 767, 821, 1054, 1081
BtgI CCRYGG 1 cut(s) 882
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 5 cut(s) 184, 355, 937, 992, 1108
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 7 cut(s) 90, 219, 252, 460, 731, 909, 1027
Cac8I GCNNGC 1 cut(s) 95
CaiI CAGNNNCTG 1 cut(s) 171
CciI TCATGA 1 cut(s) 367
CfoI GCGC 1 cut(s) 518
Cfr10I RCCGGY 1 cut(s) 662
Cfr13I GGNCC 3 cut(s) 29, 650, 806
CseI GACGC 1 cut(s) 19
Csp6I GTAC 5 cut(s) 108, 324, 610, 1010, 1059
CspAI ACCGGT 1 cut(s) 662
CviQI GTAC 5 cut(s) 108, 324, 610, 1010, 1059
DdeI CTNAG 5 cut(s) 82, 189, 263, 495, 723
DpnI GATC 5 cut(s) 441, 528, 645, 879, 890
DpnII GATC 5 cut(s) 439, 526, 643, 877, 888
DrdI GACNNNNNNGTC 1 cut(s) 1130
DseDI GACNNNNNNGTC 1 cut(s) 1130
EaeI YGGCCR 1 cut(s) 765
Eam1104I CTCTTC 1 cut(s) 1145
EarI CTCTTC 1 cut(s) 1145
Ecl136II GAGCTC 1 cut(s) 138
Eco130I CCWWGG 2 cut(s) 699, 882
Eco24I GRGCYC 1 cut(s) 140
Eco47I GGWCC 2 cut(s) 650, 806
Eco47III AGCGCT 1 cut(s) 517
Eco53kI GAGCTC 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 138
EcoRII CCWGG 1 cut(s) 808
EcoT14I CCWWGG 2 cut(s) 699, 882
EcoT38I GRGCYC 1 cut(s) 140
ErhI CCWWGG 2 cut(s) 699, 882
Esp3I CGTCTC 1 cut(s) 2
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FauNDI CATATG 1 cut(s) 672
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 2 cut(s) 237, 1046
FokI GGATG 5 cut(s) 191, 362, 924, 979, 1095
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 2 cut(s) 237, 1046
GlaI GCGC 1 cut(s) 517
GluI GCNGC 2 cut(s) 237, 1046
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 6 cut(s) 31, 153, 767, 821, 1054, 1081
HapII CCGG 3 cut(s) 321, 647, 663
HgaI GACGC 1 cut(s) 19
HhaI GCGC 1 cut(s) 518
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HincII GTYRAC 1 cut(s) 1135
HindII GTYRAC 1 cut(s) 1135
HinfI GANTC 2 cut(s) 316, 448
HpaII CCGG 3 cut(s) 321, 647, 663
HphI GGTGA 3 cut(s) 17, 29, 1009
Hpy166II GTNNAC 7 cut(s) 418, 653, 668, 732, 806, 1010, 1135
Hpy188I TCNGA 6 cut(s) 51, 439, 559, 925, 997, 1169
Hpy188III TCNNGA 9 cut(s) 211, 313, 368, 382, 443, 521, 530, 1112, 1121
Hpy8I GTNNAC 7 cut(s) 418, 653, 668, 732, 806, 1010, 1135
HpyAV CCTTC 4 cut(s) 286, 365, 468, 924
HpyCH4III ACNGT 2 cut(s) 79, 455
HpyCH4IV ACGT 3 cut(s) 34, 618, 908
HpyCH4V TGCA 5 cut(s) 25, 577, 834, 1048, 1097
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 168, 242, 1051
HpyF3I CTNAG 5 cut(s) 82, 189, 263, 495, 723
HpySE526I ACGT 3 cut(s) 34, 618, 908
HspAI GCGC 1 cut(s) 516
Kzo9I GATC 5 cut(s) 439, 526, 643, 877, 888
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 2 cut(s) 248, 1032
LweI GCATC 3 cut(s) 169, 481, 946
MaeII ACGT 3 cut(s) 34, 618, 908
MalI GATC 5 cut(s) 441, 528, 645, 879, 890
MboI GATC 5 cut(s) 439, 526, 643, 877, 888
MboII GAAGA 6 cut(s) 28, 64, 536, 716, 856, 1132
MflI RGATCY 3 cut(s) 526, 643, 888
MhlI GDGCHC 2 cut(s) 140, 724
MluCI AATT 6 cut(s) 215, 353, 377, 788, 895, 974
MlyI GAGTC 1 cut(s) 310
MmeI TCCRAC 1 cut(s) 639
MroXI GAANNNNTTC 1 cut(s) 478
MseI TTAA 1 cut(s) 1074
MslI CAYNNNNRTG 3 cut(s) 456, 1022, 1038
MspA1I CMGCKG 1 cut(s) 1163
MspI CCGG 3 cut(s) 321, 647, 663
MspR9I CCNGG 3 cut(s) 321, 648, 810
MvaI CCWGG 1 cut(s) 810
MvnI CGCG 2 cut(s) 752, 795
MwoI GCNNNNNNNGC 4 cut(s) 159, 168, 242, 1051
NciI CCSGG 2 cut(s) 321, 648
NcoI CCATGG 1 cut(s) 882
NdeI CATATG 1 cut(s) 672
NdeII GATC 5 cut(s) 439, 526, 643, 877, 888
NlaIV GGNNCC 3 cut(s) 645, 651, 661
NmuCI GTSAC 7 cut(s) 35, 85, 146, 428, 904, 988, 1015
NspI RCATGY 4 cut(s) 97, 118, 685, 1016
OliI CACNNNNGTG 1 cut(s) 1022
PaeI GCATGC 1 cut(s) 97
PaeR7I CTCGAG 1 cut(s) 240
PagI TCATGA 1 cut(s) 367
PciI ACATGT 3 cut(s) 114, 681, 1012
PcsI WCGNNNNNNNCGW 1 cut(s) 957
PdmI GAANNNNTTC 1 cut(s) 478
PfeI GAWTC 1 cut(s) 448
PflMI CCANNNNNTGG 1 cut(s) 128
PinAI ACCGGT 1 cut(s) 662
PkrI GCNGC 2 cut(s) 238, 1047
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PscI ACATGT 3 cut(s) 114, 681, 1012
Psp124BI GAGCTC 1 cut(s) 140
Psp1406I AACGTT 1 cut(s) 618
Psp6I CCWGG 1 cut(s) 808
PspGI CCWGG 1 cut(s) 808
PspN4I GGNNCC 3 cut(s) 645, 651, 661
PspPI GGNCC 3 cut(s) 29, 650, 806
PspXI VCTCGAGB 1 cut(s) 240
PsrI GAACNNNNNNTAC 2 cut(s) 669, 701
PstNI CAGNNNCTG 1 cut(s) 171
PsuI RGATCY 3 cut(s) 526, 643, 888
PvuII CAGCTG 1 cut(s) 1163
RsaI GTAC 5 cut(s) 109, 325, 611, 1011, 1060
RsaNI GTAC 5 cut(s) 108, 324, 610, 1010, 1059
RseI CAYNNNNRTG 3 cut(s) 456, 1022, 1038
SacI GAGCTC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 1074
SatI GCNGC 2 cut(s) 237, 1046
Sau3AI GATC 5 cut(s) 439, 526, 643, 877, 888
Sau96I GGNCC 3 cut(s) 29, 650, 806
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 3 cut(s) 321, 648, 810
SduI GDGCHC 2 cut(s) 140, 724
SfaNI GCATC 3 cut(s) 169, 481, 946
SfcI CTRYAG 1 cut(s) 157
Sfr274I CTCGAG 1 cut(s) 240
SinI GGWCC 2 cut(s) 650, 806
SlaI CTCGAG 1 cut(s) 240
SmiMI CAYNNNNRTG 3 cut(s) 456, 1022, 1038
SmlI CTYRAG 1 cut(s) 240
SmoI CTYRAG 1 cut(s) 240
SphI GCATGC 1 cut(s) 97
Sse9I AATT 6 cut(s) 215, 353, 377, 788, 895, 974
SsiI CCGC 2 cut(s) 502, 793
SstI GAGCTC 1 cut(s) 140
StyD4I CCNGG 3 cut(s) 319, 646, 808
StyI CCWWGG 2 cut(s) 699, 882
TaaI ACNGT 2 cut(s) 79, 455
TaiI ACGT 3 cut(s) 37, 621, 911
TaqI TCGA 4 cut(s) 241, 383, 442, 1122
TasI AATT 6 cut(s) 215, 353, 377, 788, 895, 974
TatI WGTACW 4 cut(s) 107, 609, 1009, 1058
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 1 cut(s) 1074
Tru9I TTAA 1 cut(s) 1074
TscAI CASTG 7 cut(s) 90, 226, 252, 460, 731, 909, 1027
TseFI GTSAC 7 cut(s) 35, 85, 146, 428, 904, 988, 1015
TseI GCWGC 2 cut(s) 236, 1045
Tsp45I GTSAC 7 cut(s) 35, 85, 146, 428, 904, 988, 1015
TspDTI ATGAA 9 cut(s) 194, 268, 366, 384, 616, 752, 858, 957, 995
TspGWI ACGGA 2 cut(s) 561, 966
TspRI CASTG 7 cut(s) 90, 226, 252, 460, 731, 909, 1027
Van91I CCANNNNNTGG 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 650, 806
XapI RAATTY 5 cut(s) 215, 353, 377, 788, 974
XceI RCATGY 4 cut(s) 97, 118, 685, 1016
XhoI CTCGAG 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 667
XmnI GAANNNNTTC 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.