RLG00000009229

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004280
Physical Location & Seq
Forward (+)
49399528 .. 49405207
5680 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009229

Sequence Viewer

Length: 1389 bp
ATGAAGACGGGTCAGCCGTGGCGAATAGGCATGGGTGACATTCCTATATTTTCTGGGGCTCGCCATCGCCCACCTTTGAAGAAGCCGTTGTGGATCATTGTCTTGGTGTCTTTGGTTAGCATCTTTGTGATTTGTGCTTTTCTCTATCAACCGCAAAGCAACGCCGCTTGTTACATATTTTCTTCAAGAGGTTGTAAGGCTATTTCCGATTGGCTTCCACCTGCTCCAGCAAGGGAATATAATGATGATGAGATTGCAGCCCGGGTAGTGATAAAAGAAATTTTGAGTGCGCCTCCTGTTCAATCAAAAAACCCGAAAATAGCATTCATGTTCCTTACACCAAGTGCAGTGCCTTTTGAGAAGCTGTGGGATAAATTTTTTCATGGTCATGAGGGAAAGTTCTCTGTTTATGTGCATGCATCTAAAGAAAAGGCAGTTCATTTGAGCCGTTACTTTGCTAATAGAGAGATACGCAGTGATCAGGTGATTTGGGGAAAAATTTCCATGGTAGATGCTGAGAGACGATTATTGGCAAATGCTCTTCAAGATCCTGATAACCAGCACTTTGTGCTACTTTCTGAAAGTTGTGTACCGTTGTATACATTTGACTATATCTATGACTATCTGATGAATACAAATATAAGCTATGTTGACTGCTTTGAGGATCCTGGCCCACATGGAAATGGCAGGTACTCAGAACACATGTTACCTGAAATTGAGAAGAAAGACTTTAGAAAGGGTGCACAGTGGTTCTCTGTTAAGCGTCAGCATGCTGTTATAATAATGGCTGATAGTCTTTACTACTCAAAATTCCGGGATTACTGCAAGCCAGGTCTGGAGGGGCGCAATTGCATTGCTGATGAACATTACTTGCCAACCTTTTTCAATATAGTCGATCCTGGTGGAATTGCAAACTGGTCTGTAACACATGTTGATTGGTCTGAAAGAAAGTGGCACCCAAAGTTATACAAGGCTCAGGATATTAGTTACGAACTCTTGAGAAATATTACGTTCTTGAGTGCAAGGGAGGCTGGCAGAGCAGTTGGGGTGACCAGAGTTGAGTTGGAAGCTAAAAAGTATTTGGTTGGGGAGCTTGACAGTTTATTAGAGTTGAAAAGACAAGAAGAAAGCAAACTGCAATTTTGGAAGCCATTGGAGACGAAGAAGGTTTTGGGTGAGGATCCAATATTCCAAGGGGAGCTTGGTGCGCCGTTGCGCTCCACCCAAAACCAAATCTCCCTCAATTCTATCTTGCTAGTCCGGCGGCCCTCCGCGCCGGCGCGGATGTTGGACCTTGTTTGTAAAGATGTGTCCATCGAGCTTCATAGAAGCGATGGAGGTTTTGAGTCTTGTAGGCGTGATGCAGGTGCTGCTGTACGGGCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

463

Amino Acids

52.78

Weight (kDa)

8.56

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 107 - 336 2e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 779
AarI CACCTGC 2 cut(s) 229, 1355
Acc36I ACCTGC 3 cut(s) 229, 678, 1355
AccB1I GGYRCC 1 cut(s) 954
AccI GTMKAC 1 cut(s) 599
AccII CGCG 2 cut(s) 1274, 1282
AciI CCGC 5 cut(s) 152, 165, 1264, 1272, 1282
AclWI GGATC 7 cut(s) 101, 542, 659, 672, 890, 1175, 1188
AcsI RAATTY 4 cut(s) 279, 374, 498, 809
AdeI CACNNNGTG 2 cut(s) 344, 568
AfaI GTAC 3 cut(s) 591, 692, 1377
AflIII ACRYGT 2 cut(s) 702, 928
AgsI TTSAA 6 cut(s) 79, 186, 302, 545, 886, 1114
AjnI CCWGG 3 cut(s) 667, 829, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
AluBI AGCT 6 cut(s) 364, 645, 1070, 1093, 1201, 1321
AluI AGCT 6 cut(s) 364, 645, 1070, 1093, 1201, 1321
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 2 cut(s) 514, 1151
Alw44I GTGCAC 1 cut(s) 741
AlwI GGATC 7 cut(s) 101, 542, 659, 672, 890, 1175, 1188
AlwNI CAGNNNCTG 1 cut(s) 1370
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 2 cut(s) 670, 1265
ApaLI GTGCAC 1 cut(s) 741
ApeKI GCWGC 2 cut(s) 257, 1370
ApoI RAATTY 4 cut(s) 279, 374, 498, 809
Asp700I GAANNNNTTC 1 cut(s) 499
AspLEI GCGC 6 cut(s) 292, 846, 1210, 1218, 1276, 1282
AspS9I GGNCC 3 cut(s) 671, 1266, 1291
AsuC2I CCSGG 3 cut(s) 262, 263, 815
AsuHPI GGTGA 4 cut(s) 47, 496, 1060, 1187
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 1291
BaeGI GKGCMC 1 cut(s) 745
BamHI GGATCC 2 cut(s) 664, 1180
BanI GGYRCC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 745
BbvI GCAGC 2 cut(s) 269, 1357
BccI CCATC 3 cut(s) 72, 1322, 1328
BceAI ACGGC 3 cut(s) 70, 432, 1195
BciT130I CCWGG 3 cut(s) 669, 831, 900
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 3 cut(s) 262, 263, 815
BcoDI GTCTC 2 cut(s) 514, 1151
BfaI CTAG 1 cut(s) 1256
BfuAI ACCTGC 3 cut(s) 229, 678, 1355
BisI GCNGC 4 cut(s) 165, 258, 1265, 1371
BlsI GCNGC 4 cut(s) 166, 259, 1266, 1372
Bme1390I CCNGG 6 cut(s) 262, 263, 669, 815, 831, 900
Bme18I GGWCC 1 cut(s) 1291
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 3 cut(s) 671, 1266, 1291
BmiI GGNNCC 3 cut(s) 666, 956, 1182
BmrFI CCNGG 6 cut(s) 262, 263, 669, 815, 831, 900
BmsI GCATC 4 cut(s) 129, 428, 502, 1351
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 2 cut(s) 210, 857
Bpu10I CCTNAGC 1 cut(s) 975
BpuEI CTTGAG 2 cut(s) 1018, 1036
BpuMI CCSGG 3 cut(s) 262, 263, 815
BsaJI CCNNGG 4 cut(s) 17, 261, 504, 1192
BsaXI ACNNNNNCTCC 2 cut(s) 1220, 1250
Bse118I RCCGGY 1 cut(s) 1276
Bse1I ACTGG 1 cut(s) 920
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 3 cut(s) 669, 831, 900
BseDI CCNNGG 4 cut(s) 17, 261, 504, 1192
BseGI GGATG 1 cut(s) 1290
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 3 cut(s) 507, 708, 989
BseNI ACTGG 1 cut(s) 920
BseSI GKGCMC 1 cut(s) 745
BseXI GCAGC 2 cut(s) 269, 1357
BsgI GTGCAG 1 cut(s) 366
Bsh1236I CGCG 2 cut(s) 1274, 1282
BshFI GGCC 2 cut(s) 672, 1267
BshNI GGYRCC 1 cut(s) 954
BsiHKAI GWGCWC 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 4 cut(s) 262, 814, 1261, 1277
BsmAI GTCTC 2 cut(s) 514, 1151
BsmBI CGTCTC 2 cut(s) 514, 1151
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 2 cut(s) 672, 1267
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 2 cut(s) 61, 745
Bsp143I GATC 6 cut(s) 93, 478, 547, 664, 895, 1180
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 5 cut(s) 152, 165, 1264, 1272, 1282
BspANI GGCC 2 cut(s) 672, 1267
BspCNI CTCAG 3 cut(s) 508, 707, 988
BspFNI CGCG 2 cut(s) 1274, 1282
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 3 cut(s) 666, 956, 1182
BspMI ACCTGC 3 cut(s) 229, 678, 1355
BspPI GGATC 7 cut(s) 101, 542, 659, 672, 890, 1175, 1188
BspQI GCTCTTC 1 cut(s) 546
BspT107I GGYRCC 1 cut(s) 954
BsrDI GCAATG 1 cut(s) 852
BsrFI RCCGGY 1 cut(s) 1276
BsrI ACTGG 1 cut(s) 920
BssAI RCCGGY 1 cut(s) 1276
BssECI CCNNGG 4 cut(s) 17, 261, 504, 1192
BssMI GATC 6 cut(s) 93, 478, 547, 664, 895, 1180
BssNAI GTATAC 1 cut(s) 600
BssT1I CCWWGG 2 cut(s) 504, 1192
Bst1107I GTATAC 1 cut(s) 600
Bst2UI CCWGG 3 cut(s) 669, 831, 900
Bst4CI ACNGT 3 cut(s) 594, 747, 1100
Bst6I CTCTTC 1 cut(s) 546
BstAPI GCANNNNNTGC 2 cut(s) 568, 1370
BstC8I GCNNGC 6 cut(s) 61, 417, 771, 827, 1033, 1278
BstDEI CTNAG 3 cut(s) 516, 694, 975
BstDSI CCRYGG 2 cut(s) 17, 504
BstEII GGTNACC 1 cut(s) 1048
BstF5I GGATG 1 cut(s) 1290
BstFNI CGCG 2 cut(s) 1274, 1282
BstHHI GCGC 6 cut(s) 292, 846, 1210, 1218, 1276, 1282
BstKTI GATC 6 cut(s) 96, 481, 550, 667, 898, 1183
BstMAI GTCTC 2 cut(s) 514, 1151
BstMBI GATC 6 cut(s) 93, 478, 547, 664, 895, 1180
BstMWI GCNNNNNNNGC 8 cut(s) 568, 1028, 1037, 1207, 1261, 1273, 1370, 1379
BstNI CCWGG 3 cut(s) 669, 831, 900
BstNSI RCATGY 4 cut(s) 419, 706, 773, 932
BstPI GGTNACC 1 cut(s) 1048
BstSCI CCNGG 6 cut(s) 260, 261, 667, 813, 829, 898
BstSLI GKGCMC 1 cut(s) 745
BstUI CGCG 2 cut(s) 1274, 1282
BstV1I GCAGC 2 cut(s) 269, 1357
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 3 cut(s) 547, 664, 1180
BstYI RGATCY 3 cut(s) 547, 664, 1180
BstZ17I GTATAC 1 cut(s) 600
BsuRI GGCC 2 cut(s) 672, 1267
BtgI CCRYGG 2 cut(s) 17, 504
BtgZI GCGATG 2 cut(s) 50, 1347
BtsCI GGATG 1 cut(s) 1290
BtsI GCAGTG 2 cut(s) 354, 481
BtsIMutI CAGTG 3 cut(s) 354, 481, 752
BveI ACCTGC 3 cut(s) 229, 678, 1355
Cac8I GCNNGC 6 cut(s) 61, 417, 771, 827, 1033, 1278
CaiI CAGNNNCTG 1 cut(s) 1370
CciI TCATGA 1 cut(s) 388
CfoI GCGC 6 cut(s) 292, 846, 1210, 1218, 1276, 1282
Cfr10I RCCGGY 1 cut(s) 1276
Cfr13I GGNCC 3 cut(s) 671, 1266, 1291
Cfr9I CCCGGG 1 cut(s) 261
CseI GACGC 1 cut(s) 752
Csp6I GTAC 3 cut(s) 590, 691, 1376
CviQI GTAC 3 cut(s) 590, 691, 1376
DdeI CTNAG 3 cut(s) 516, 694, 975
DpnI GATC 6 cut(s) 95, 480, 549, 666, 897, 1182
DpnII GATC 6 cut(s) 93, 478, 547, 664, 895, 1180
DraIII CACNNNGTG 2 cut(s) 344, 568
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
Eco130I CCWWGG 2 cut(s) 504, 1192
Eco24I GRGCYC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 1291
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 1048
EcoO65I GGTNACC 1 cut(s) 1048
EcoRII CCWGG 3 cut(s) 667, 829, 898
EcoT14I CCWWGG 2 cut(s) 504, 1192
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 2 cut(s) 504, 1192
Esp3I CGTCTC 2 cut(s) 514, 1151
FalI AAGNNNNNCTT 4 cut(s) 713, 745, 1185, 1217
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 4 cut(s) 165, 258, 1265, 1371
FokI GGATG 1 cut(s) 1297
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 4 cut(s) 165, 258, 1265, 1371
FspBI CTAG 1 cut(s) 1256
GlaI GCGC 6 cut(s) 291, 845, 1209, 1217, 1275, 1281
GluI GCNGC 4 cut(s) 165, 258, 1265, 1371
GsuI CTGGAG 2 cut(s) 210, 857
HaeIII GGCC 2 cut(s) 672, 1267
HapII CCGG 4 cut(s) 262, 814, 1261, 1277
HgaI GACGC 1 cut(s) 752
HhaI GCGC 6 cut(s) 292, 846, 1210, 1218, 1276, 1282
Hin6I GCGC 6 cut(s) 290, 844, 1208, 1216, 1274, 1280
HinP1I GCGC 6 cut(s) 290, 844, 1208, 1216, 1274, 1280
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 1 cut(s) 1346
HpaII CCGG 4 cut(s) 262, 814, 1261, 1277
HphI GGTGA 4 cut(s) 47, 496, 1060, 1187
Hpy166II GTNNAC 4 cut(s) 590, 600, 652, 743
Hpy188I TCNGA 5 cut(s) 208, 580, 627, 697, 943
Hpy188III TCNNGA 8 cut(s) 186, 389, 545, 551, 836, 977, 997, 1015
Hpy8I GTNNAC 4 cut(s) 590, 600, 652, 743
HpyAV CCTTC 1 cut(s) 1159
HpyCH4III ACNGT 3 cut(s) 594, 747, 1100
HpyCH4IV ACGT 1 cut(s) 1010
HpyF10VI GCNNNNNNNGC 8 cut(s) 568, 1028, 1037, 1207, 1261, 1273, 1370, 1379
HpyF3I CTNAG 3 cut(s) 516, 694, 975
HpySE526I ACGT 1 cut(s) 1010
HspAI GCGC 6 cut(s) 290, 844, 1208, 1216, 1274, 1280
KroI GCCGGC 1 cut(s) 1276
KroNI GCCGGC 1 cut(s) 1278
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 93, 478, 547, 664, 895, 1180
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 4 cut(s) 229, 1090, 1198, 1223
Lsp1109I GCAGC 2 cut(s) 269, 1357
LweI GCATC 4 cut(s) 129, 428, 502, 1351
MaeI CTAG 1 cut(s) 1256
MaeII ACGT 1 cut(s) 1010
MaeIII GTNAC 7 cut(s) 35, 170, 449, 705, 922, 986, 1048
MalI GATC 6 cut(s) 95, 480, 549, 666, 897, 1182
MboI GATC 6 cut(s) 93, 478, 547, 664, 895, 1180
MboII GAAGA 7 cut(s) 16, 91, 174, 533, 733, 1136, 1174
MfeI CAATTG 1 cut(s) 847
MflI RGATCY 3 cut(s) 547, 664, 1180
MhlI GDGCHC 2 cut(s) 61, 745
MluCI AATT 9 cut(s) 279, 374, 498, 714, 809, 847, 906, 1139, 1243
MlyI GAGTC 1 cut(s) 1355
MmeI TCCRAC 2 cut(s) 1044, 1269
Mph1103I ATGCAT 1 cut(s) 421
MreI CGCCGGCG 1 cut(s) 1276
MroNI GCCGGC 1 cut(s) 1276
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 1 cut(s) 759
MslI CAYNNNNRTG 3 cut(s) 125, 387, 681
MspI CCGG 4 cut(s) 262, 814, 1261, 1277
MspR9I CCNGG 6 cut(s) 262, 263, 669, 815, 831, 900
MunI CAATTG 1 cut(s) 847
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 3 cut(s) 669, 831, 900
MvnI CGCG 2 cut(s) 1274, 1282
MwoI GCNNNNNNNGC 8 cut(s) 568, 1028, 1037, 1207, 1261, 1273, 1370, 1379
NaeI GCCGGC 1 cut(s) 1278
NciI CCSGG 3 cut(s) 262, 263, 815
NcoI CCATGG 1 cut(s) 504
NdeII GATC 6 cut(s) 93, 478, 547, 664, 895, 1180
NgoMIV GCCGGC 1 cut(s) 1276
NlaIV GGNNCC 3 cut(s) 666, 956, 1182
NmuCI GTSAC 2 cut(s) 35, 1048
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 4 cut(s) 419, 706, 773, 932
PaeI GCATGC 2 cut(s) 419, 773
PagI TCATGA 1 cut(s) 388
PaqCI CACCTGC 2 cut(s) 229, 1355
PciI ACATGT 2 cut(s) 702, 928
PciSI GCTCTTC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 323
PdiI GCCGGC 1 cut(s) 1278
PdmI GAANNNNTTC 1 cut(s) 499
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 4 cut(s) 166, 259, 1266, 1372
PleI GAGTC 1 cut(s) 1354
PpsI GAGTC 1 cut(s) 1354
PscI ACATGT 2 cut(s) 702, 928
PsiI TTATAA 1 cut(s) 779
Psp6I CCWGG 3 cut(s) 667, 829, 898
PspEI GGTNACC 1 cut(s) 1048
PspGI CCWGG 3 cut(s) 667, 829, 898
PspN4I GGNNCC 3 cut(s) 666, 956, 1182
PspPI GGNCC 3 cut(s) 671, 1266, 1291
PsrI GAACNNNNNNTAC 2 cut(s) 690, 722
PstNI CAGNNNCTG 1 cut(s) 1370
PsuI RGATCY 3 cut(s) 547, 664, 1180
RsaI GTAC 3 cut(s) 591, 692, 1377
RsaNI GTAC 3 cut(s) 590, 691, 1376
RseI CAYNNNNRTG 3 cut(s) 125, 387, 681
SapI GCTCTTC 1 cut(s) 546
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 4 cut(s) 165, 258, 1265, 1371
Sau3AI GATC 6 cut(s) 93, 478, 547, 664, 895, 1180
Sau96I GGNCC 3 cut(s) 671, 1266, 1291
SchI GAGTC 1 cut(s) 1355
ScrFI CCNGG 6 cut(s) 262, 263, 669, 815, 831, 900
SduI GDGCHC 2 cut(s) 61, 745
SfaNI GCATC 4 cut(s) 129, 428, 502, 1351
SgrAI CRCCGGYG 1 cut(s) 1276
SinI GGWCC 1 cut(s) 1291
SmaI CCCGGG 1 cut(s) 263
SmiMI CAYNNNNRTG 3 cut(s) 125, 387, 681
SmlI CTYRAG 2 cut(s) 997, 1015
SmoI CTYRAG 2 cut(s) 997, 1015
SphI GCATGC 2 cut(s) 419, 773
Sse9I AATT 9 cut(s) 279, 374, 498, 714, 809, 847, 906, 1139, 1243
SsiI CCGC 5 cut(s) 152, 165, 1264, 1272, 1282
SspI AATATT 2 cut(s) 1006, 1188
SspMI CTAG 1 cut(s) 1256
StyD4I CCNGG 6 cut(s) 260, 261, 667, 813, 829, 898
StyI CCWWGG 2 cut(s) 504, 1192
TaaI ACNGT 3 cut(s) 594, 747, 1100
TaiI ACGT 1 cut(s) 1013
TaqI TCGA 2 cut(s) 894, 1317
TasI AATT 9 cut(s) 279, 374, 498, 714, 809, 847, 906, 1139, 1243
TauI GCSGC 2 cut(s) 167, 1267
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 3 cut(s) 354, 481, 752
TseFI GTSAC 2 cut(s) 35, 1048
TseI GCWGC 2 cut(s) 257, 1370
Tsp45I GTSAC 2 cut(s) 35, 1048
TspDTI ATGAA 7 cut(s) 17, 316, 371, 428, 644, 876, 1313
TspMI CCCGGG 1 cut(s) 261
TspRI CASTG 3 cut(s) 354, 481, 752
VneI GTGCAC 1 cut(s) 741
VpaK11BI GGWCC 1 cut(s) 1291
XapI RAATTY 4 cut(s) 279, 374, 498, 809
XceI RCATGY 4 cut(s) 419, 706, 773, 932
XcmI CCANNNNNNNNNTGG 2 cut(s) 1060, 1199
XmaI CCCGGG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 599
XmnI GAANNNNTTC 1 cut(s) 499
XspI CTAG 1 cut(s) 1256
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.