AT5G57270

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004280
Physical Location & Seq
Reverse (-)
23200591 .. 23203908
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57270.5

Sequence Viewer

Length: 1167 bp
ATGGGAGAGAATCTGCATTTGTCTCCACGACACCGATCTTCTCTAAAGAAGCCATTGTTGATTGTTTTGTTAGTCTGTATCACAAGTGTGCTTTTGGTGATCACTTACATGTATCCACAACACAACAACAGTAAGAGCTCTGCTTGTGCGGGTTTGTCTAGTAGAGGCTGTCAAGCTGCGCTCTCTGGATGGCTTCCTGTTCATGTCAGGAAATTCACTGATGAAGAAGTAGCAGCTCGAGTTGTGATCAAAGATATACTGAGACTACCTCCTGCTCTAACCGCTAAATCGAAAATCGCTTTTATGTTTTTGACTCCTGGTACTTTGCCATTTGAGAAGCTTTGGGATAAGTTTTTCCAGGGTCAAGAAGGAAGATTCTCTATTTACATCCACCCTTCCAGGTTACGACCAGTTCACATTAGCCGTCACTTTTCAGACCGTGAAATTCATAGTGATCATGTCACTTGGGGAAGGATTTCTATGGTTGATGCTGAGAGACGGCTTCTAGCAAATGCTCTTGAAGATCCTGATAACCAGCACTTTGTTCTTCTTTCAGAAAGTTGTATACCGTTGCATACATTTGACTACACCTATCGATATTTGATGCACGCAAATGTCAGCTTCATTGATAGTTTTGAGGATCTTGGTCCTCATGGGACTGGCAGACACATGGATCACATGTTACCTGAGATCCCAAGGCAAGATTTCAGAAAGGGTGCACAGTGGTTCACAATGAAGCGTCAACACGCTGTAATAGTGATGGCTGATGGTCTCTACTACTCGAAATTCCGGGAGTATTGCAGACCTGGAGTAGAAGCAAACAAGAACTGCATCGCGGATGAACATTACCTGCCAACATTCTTTCATATGCTTGATCCTGGAGGAATCTCGAACTGGTCAGTCACATATGTTGATTGGTCTGAGCGTAGATGGCATCCAAAGACATATAGAGCACGCGATGTCTCACTCAAGCTCTTGAAAAATATCACGTCTGACGACATGAGTGTACATGTAACAAGCGTTGGCAAGCGCGGAGAAGAGCTGCGTTGGCCTTGTACATGGAAGGGAATTAGACGACCATGTTATCTATTTGCAAGGAAGCTTCACTCAGATGCTCTTTACAAACTGGTCAGACTCTTTCCAAATTACACAAGCACAGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

388

Amino Acids

44.97

Weight (kDa)

9.35

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 99 - 329 9.8e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 858
AccI GTMKAC 1 cut(s) 565
AccII CGCG 3 cut(s) 836, 957, 1032
AciI CCGC 4 cut(s) 149, 282, 836, 1032
AclWI GGATC 5 cut(s) 518, 648, 681, 685, 869
AcsI RAATTY 3 cut(s) 212, 444, 785
AfaI GTAC 3 cut(s) 322, 1008, 1057
AflIII ACRYGT 3 cut(s) 108, 678, 1009
AgsI TTSAA 2 cut(s) 521, 979
AjiI CACGTC 1 cut(s) 990
AjnI CCWGG 5 cut(s) 316, 357, 398, 805, 877
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 8 cut(s) 138, 176, 236, 340, 621, 973, 1042, 1102
AluI AGCT 8 cut(s) 138, 176, 236, 340, 621, 973, 1042, 1102
Alw21I GWGCWC 3 cut(s) 140, 721, 955
Alw26I GTCTC 5 cut(s) 27, 256, 490, 776, 967
Alw44I GTGCAC 1 cut(s) 717
AlwI GGATC 5 cut(s) 518, 648, 681, 685, 869
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 1 cut(s) 1049
ApaLI GTGCAC 1 cut(s) 717
ApeKI GCWGC 3 cut(s) 176, 233, 1042
ApoI RAATTY 3 cut(s) 212, 444, 785
Asp700I GAANNNNTTC 1 cut(s) 475
AspLEI GCGC 2 cut(s) 181, 1032
AspS9I GGNCC 1 cut(s) 647
AsuC2I CCSGG 1 cut(s) 791
AsuHPI GGTGA 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 721
BanII GRGCYC 1 cut(s) 140
Bbv12I GWGCWC 3 cut(s) 140, 721, 955
BbvI GCAGC 3 cut(s) 163, 245, 1029
BccI CCATC 4 cut(s) 183, 754, 761, 924
BceAI ACGGC 2 cut(s) 408, 515
BciT130I CCWGG 5 cut(s) 318, 359, 400, 807, 879
BciVI GTATCC 1 cut(s) 123
BclI TGATCA 3 cut(s) 99, 246, 454
BcnI CCSGG 1 cut(s) 791
BcoDI GTCTC 5 cut(s) 27, 256, 490, 776, 967
BfaI CTAG 2 cut(s) 159, 506
BfuAI ACCTGC 1 cut(s) 858
BfuI GTATCC 1 cut(s) 123
BisI GCNGC 3 cut(s) 177, 234, 1043
BlsI GCNGC 3 cut(s) 178, 235, 1044
Bme1390I CCNGG 6 cut(s) 318, 359, 400, 791, 807, 879
Bme18I GGWCC 1 cut(s) 647
BmeT110I CYCGRG 1 cut(s) 237
BmgBI CACGTC 1 cut(s) 990
BmgT120I GGNCC 1 cut(s) 647
BmrFI CCNGG 6 cut(s) 318, 359, 400, 791, 807, 879
BmsI GCATC 5 cut(s) 478, 594, 840, 943, 1102
BplI GAGNNNNNCTC 2 cut(s) 253, 285
BpmI CTGGAG 2 cut(s) 828, 900
BpuEI CTTGAG 1 cut(s) 953
BpuMI CCSGG 1 cut(s) 791
Bsa29I ATCGAT 1 cut(s) 595
BsaI GGTCTC 1 cut(s) 776
BsaJI CCNNGG 2 cut(s) 358, 695
Bse1I ACTGG 4 cut(s) 410, 664, 899, 1131
BseBI CCWGG 5 cut(s) 318, 359, 400, 807, 879
BseCI ATCGAT 1 cut(s) 595
BseDI CCNNGG 2 cut(s) 358, 695
BseGI GGATG 4 cut(s) 194, 387, 844, 934
BseMII CTCAG 5 cut(s) 251, 483, 678, 912, 1122
BseNI ACTGG 4 cut(s) 410, 664, 899, 1131
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 3 cut(s) 163, 245, 1029
Bsh1236I CGCG 3 cut(s) 836, 957, 1032
BshFI GGCC 1 cut(s) 1051
BshVI ATCGAT 1 cut(s) 595
BsiHKAI GWGCWC 3 cut(s) 140, 721, 955
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 1 cut(s) 790
BslFI GGGAC 1 cut(s) 670
BsmAI GTCTC 5 cut(s) 27, 256, 490, 776, 967
BsmBI CGTCTC 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 670
BsnI GGCC 1 cut(s) 1051
Bso31I GGTCTC 1 cut(s) 776
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 3 cut(s) 140, 721, 955
Bsp1407I TGTACA 2 cut(s) 1006, 1055
Bsp143I GATC 9 cut(s) 35, 99, 246, 454, 523, 640, 673, 690, 874
BspACI CCGC 4 cut(s) 149, 282, 836, 1032
BspANI GGCC 1 cut(s) 1051
BspCNI CTCAG 5 cut(s) 252, 484, 679, 913, 1121
BspDI ATCGAT 1 cut(s) 595
BspFNI CGCG 3 cut(s) 836, 957, 1032
BspMI ACCTGC 1 cut(s) 858
BspPI GGATC 5 cut(s) 518, 648, 681, 685, 869
BspQI GCTCTTC 1 cut(s) 1032
BspTNI GGTCTC 1 cut(s) 776
BsrGI TGTACA 2 cut(s) 1006, 1055
BsrI ACTGG 4 cut(s) 410, 664, 899, 1131
BssECI CCNNGG 2 cut(s) 358, 695
BssMI GATC 9 cut(s) 35, 99, 246, 454, 523, 640, 673, 690, 874
BssNAI GTATAC 1 cut(s) 566
BssT1I CCWWGG 1 cut(s) 695
Bst1107I GTATAC 1 cut(s) 566
Bst2UI CCWGG 5 cut(s) 318, 359, 400, 807, 879
Bst4CI ACNGT 5 cut(s) 131, 440, 570, 723, 1159
Bst6I CTCTTC 1 cut(s) 1032
BstAUI TGTACA 2 cut(s) 1006, 1055
BstC8I GCNNGC 3 cut(s) 609, 955, 1028
BstDEI CTNAG 5 cut(s) 260, 492, 687, 921, 1108
BstF5I GGATG 4 cut(s) 194, 387, 844, 934
BstFNI CGCG 3 cut(s) 836, 957, 1032
BstHHI GCGC 2 cut(s) 181, 1032
BstKTI GATC 9 cut(s) 38, 102, 249, 457, 526, 643, 676, 693, 877
BstMAI GTCTC 5 cut(s) 27, 256, 490, 776, 967
BstMBI GATC 9 cut(s) 35, 99, 246, 454, 523, 640, 673, 690, 874
BstMWI GCNNNNNNNGC 3 cut(s) 281, 931, 1048
BstNI CCWGG 5 cut(s) 318, 359, 400, 807, 879
BstNSI RCATGY 3 cut(s) 112, 682, 1013
BstSCI CCNGG 6 cut(s) 316, 357, 398, 789, 805, 877
BstSLI GKGCMC 1 cut(s) 721
BstUI CGCG 3 cut(s) 836, 957, 1032
BstV1I GCAGC 3 cut(s) 163, 245, 1029
BstX2I RGATCY 3 cut(s) 523, 640, 690
BstYI RGATCY 3 cut(s) 523, 640, 690
BstZ17I GTATAC 1 cut(s) 566
Bsu15I ATCGAT 1 cut(s) 595
BsuI GTATCC 1 cut(s) 123
BsuRI GGCC 1 cut(s) 1051
BsuTUI ATCGAT 1 cut(s) 595
BtgZI GCGATG 2 cut(s) 817, 972
BtrI CACGTC 1 cut(s) 990
BtsCI GGATG 4 cut(s) 194, 387, 844, 934
BtsIMutI CAGTG 3 cut(s) 216, 728, 1164
BveI ACCTGC 1 cut(s) 858
Cac8I GCNNGC 3 cut(s) 609, 955, 1028
CfoI GCGC 2 cut(s) 181, 1032
Cfr13I GGNCC 1 cut(s) 647
ClaI ATCGAT 1 cut(s) 595
CseI GACGC 1 cut(s) 728
Csp6I GTAC 3 cut(s) 321, 1007, 1056
CspCI CAANNNNNGTGG 1 cut(s) 1140
CviQI GTAC 3 cut(s) 321, 1007, 1056
DdeI CTNAG 5 cut(s) 260, 492, 687, 921, 1108
DpnI GATC 9 cut(s) 37, 101, 248, 456, 525, 642, 675, 692, 876
DpnII GATC 9 cut(s) 35, 99, 246, 454, 523, 640, 673, 690, 874
Eam1104I CTCTTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 1032
Ecl136II GAGCTC 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 695
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 1 cut(s) 776
Eco47I GGWCC 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 138
EcoRII CCWGG 5 cut(s) 316, 357, 398, 805, 877
EcoT14I CCWWGG 1 cut(s) 695
EcoT38I GRGCYC 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 695
Esp3I CGTCTC 1 cut(s) 490
FaqI GGGAC 1 cut(s) 670
FauI CCCGC 1 cut(s) 142
FauNDI CATATG 2 cut(s) 867, 907
FbaI TGATCA 3 cut(s) 99, 246, 454
FblI GTMKAC 1 cut(s) 565
Fnu4HI GCNGC 3 cut(s) 177, 234, 1043
FokI GGATG 4 cut(s) 201, 374, 851, 921
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 3 cut(s) 177, 234, 1043
FspBI CTAG 2 cut(s) 159, 506
GlaI GCGC 2 cut(s) 180, 1031
GluI GCNGC 3 cut(s) 177, 234, 1043
GsuI CTGGAG 2 cut(s) 828, 900
HaeIII GGCC 1 cut(s) 1051
HapII CCGG 1 cut(s) 790
HgaI GACGC 1 cut(s) 728
HhaI GCGC 2 cut(s) 181, 1032
Hin6I GCGC 2 cut(s) 179, 1030
HinP1I GCGC 2 cut(s) 179, 1030
HincII GTYRAC 1 cut(s) 743
HindII GTYRAC 1 cut(s) 743
HindIII AAGCTT 2 cut(s) 338, 1100
HinfI GANTC 5 cut(s) 10, 313, 375, 885, 1134
HpaII CCGG 1 cut(s) 790
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 6 cut(s) 415, 566, 719, 729, 743, 1007
Hpy188I TCNGA 7 cut(s) 436, 556, 710, 922, 994, 1111, 1133
Hpy188III TCNNGA 7 cut(s) 186, 208, 365, 518, 527, 889, 976
Hpy8I GTNNAC 6 cut(s) 415, 566, 719, 729, 743, 1007
HpyAV CCTTC 4 cut(s) 362, 405, 465, 1057
HpyCH4III ACNGT 5 cut(s) 131, 440, 570, 723, 1159
HpyCH4IV ACGT 1 cut(s) 989
HpyCH4V TGCA 7 cut(s) 16, 574, 607, 719, 801, 831, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 931, 1048
HpyF3I CTNAG 5 cut(s) 260, 492, 687, 921, 1108
HpySE526I ACGT 1 cut(s) 989
HspAI GCGC 2 cut(s) 179, 1030
Ksp22I TGATCA 3 cut(s) 99, 246, 454
Kzo9I GATC 9 cut(s) 35, 99, 246, 454, 523, 640, 673, 690, 874
LguI GCTCTTC 1 cut(s) 1032
Lsp1109I GCAGC 3 cut(s) 163, 245, 1029
LweI GCATC 5 cut(s) 478, 594, 840, 943, 1102
MaeI CTAG 2 cut(s) 159, 506
MaeII ACGT 1 cut(s) 989
MaeIII GTNAC 6 cut(s) 402, 425, 460, 681, 901, 1012
MalI GATC 9 cut(s) 37, 101, 248, 456, 525, 642, 675, 692, 876
MboI GATC 9 cut(s) 35, 99, 246, 454, 523, 640, 673, 690, 874
MboII GAAGA 6 cut(s) 30, 236, 384, 533, 539, 1049
MflI RGATCY 3 cut(s) 523, 640, 690
MhlI GDGCHC 3 cut(s) 140, 721, 955
MluCI AATT 5 cut(s) 212, 444, 785, 1068, 1144
MlyI GAGTC 2 cut(s) 307, 1128
MnlI CCTC 5 cut(s) 158, 279, 631, 660, 875
MroXI GAANNNNTTC 1 cut(s) 475
MslI CAYNNNNRTG 4 cut(s) 86, 107, 612, 1110
MspI CCGG 1 cut(s) 790
MspR9I CCNGG 6 cut(s) 318, 359, 400, 791, 807, 879
MvaI CCWGG 5 cut(s) 318, 359, 400, 807, 879
MvnI CGCG 3 cut(s) 836, 957, 1032
MwoI GCNNNNNNNGC 3 cut(s) 281, 931, 1048
NciI CCSGG 1 cut(s) 791
NdeI CATATG 2 cut(s) 867, 907
NdeII GATC 9 cut(s) 35, 99, 246, 454, 523, 640, 673, 690, 874
NmuCI GTSAC 3 cut(s) 425, 460, 901
NspI RCATGY 3 cut(s) 112, 682, 1013
OliI CACNNNNGTG 1 cut(s) 86
PaeR7I CTCGAG 1 cut(s) 237
PciI ACATGT 3 cut(s) 108, 678, 1009
PciSI GCTCTTC 1 cut(s) 1032
PdmI GAANNNNTTC 1 cut(s) 475
PfeI GAWTC 3 cut(s) 10, 375, 885
PfoI TCCNGGA 2 cut(s) 789, 877
PkrI GCNGC 3 cut(s) 178, 235, 1044
PleI GAGTC 2 cut(s) 307, 1128
PpsI GAGTC 2 cut(s) 307, 1128
PscI ACATGT 3 cut(s) 108, 678, 1009
Psp124BI GAGCTC 1 cut(s) 140
Psp6I CCWGG 5 cut(s) 316, 357, 398, 805, 877
PspGI CCWGG 5 cut(s) 316, 357, 398, 805, 877
PspPI GGNCC 1 cut(s) 647
PspXI VCTCGAGB 1 cut(s) 237
PsuI RGATCY 3 cut(s) 523, 640, 690
RsaI GTAC 3 cut(s) 322, 1008, 1057
RsaNI GTAC 3 cut(s) 321, 1007, 1056
RseI CAYNNNNRTG 4 cut(s) 86, 107, 612, 1110
SacI GAGCTC 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 1032
SatI GCNGC 3 cut(s) 177, 234, 1043
Sau3AI GATC 9 cut(s) 35, 99, 246, 454, 523, 640, 673, 690, 874
Sau96I GGNCC 1 cut(s) 647
SchI GAGTC 2 cut(s) 307, 1128
ScrFI CCNGG 6 cut(s) 318, 359, 400, 791, 807, 879
SduI GDGCHC 3 cut(s) 140, 721, 955
SfaNI GCATC 5 cut(s) 478, 594, 840, 943, 1102
Sfr274I CTCGAG 1 cut(s) 237
SinI GGWCC 1 cut(s) 647
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 4 cut(s) 86, 107, 612, 1110
SmlI CTYRAG 2 cut(s) 237, 968
SmoI CTYRAG 2 cut(s) 237, 968
Sse9I AATT 5 cut(s) 212, 444, 785, 1068, 1144
SsiI CCGC 4 cut(s) 149, 282, 836, 1032
SspMI CTAG 2 cut(s) 159, 506
SstI GAGCTC 1 cut(s) 140
StyD4I CCNGG 6 cut(s) 316, 357, 398, 789, 805, 877
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 5 cut(s) 131, 440, 570, 723, 1159
TaiI ACGT 1 cut(s) 992
TaqI TCGA 5 cut(s) 238, 290, 595, 782, 890
TasI AATT 5 cut(s) 212, 444, 785, 1068, 1144
TatI WGTACW 2 cut(s) 1006, 1055
TfiI GAWTC 3 cut(s) 10, 375, 885
TscAI CASTG 3 cut(s) 223, 728, 1164
TseFI GTSAC 3 cut(s) 425, 460, 901
TseI GCWGC 3 cut(s) 176, 233, 1042
Tsp45I GTSAC 3 cut(s) 425, 460, 901
TspDTI ATGAA 7 cut(s) 191, 237, 437, 613, 749, 854, 855
TspRI CASTG 3 cut(s) 223, 728, 1164
VneI GTGCAC 1 cut(s) 717
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 3 cut(s) 212, 444, 785
XceI RCATGY 3 cut(s) 112, 682, 1013
XhoI CTCGAG 1 cut(s) 237
XmiI GTMKAC 1 cut(s) 565
XmnI GAANNNNTTC 1 cut(s) 475
XspI CTAG 2 cut(s) 159, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.