MD16G1251400.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004280
Physical Location & Seq
Reverse (-)
28155458 .. 28161161
5704 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1251400.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGAAGACGAATCAGTGGCGTCTAGGCATGGGTGACATGCAGATCTTGCCCGGGCCTCGCCATCGCCCGCCTTTGAAGAGGCCGGTGTGGATTATTGTCTTGGTCTCTTTGGTCAGCATCTTTGTGATTTGTGCTTTCCTCTATCCGCCTCAAAGCAGCGCTGCTTGTTACATATTTTCTTCTAGAGGTTGTAAGGCTATTTCGGACTGGCTTCCACCTGCTCCTGCAAGGGATTTTACCGACGATGAGATCGCATCCCGTGTTGTAATTAGAGAGATTTTAAGCCAACCTCCTATTCAATCCAGGAATCCAAAAATTGCATTCATGTTCCTTACTCCAAGTGCATTGCCTTTTGAGAAGCTCTGGGATAAGTTTCTCCATGGTCATGAAGGAAGGTTCTCTGTGTATGTCCATGCTTCTAAAGAAAAGGCAATTCATTTGAGCCGTTACTTTTCTAATCGAGAGATTCGCAGTGACCCGGTGATTTGGGGAAAAATTTCAATGGTAGATGCAGAGAGAAGATTATTGGCAAATGCTCTTCAAGATCCTGATAACCAGCATTTTGTCTTACTTTCTGAAAGTTGTGTACCATTGTACACGTTTGACTATATCTATGACTATCTGATGAATACAAATATAAGCTATGTTGACTGCTTTGAGGATCCTGGTCCTCATGGAAACGGGAGGTATTCTGAACACATGTTACCTGAAATTGAGAAGAAAGACTTTAGAAAGGGAGCACAGTGGTTCTCTATGAAGCGTCAGCATGCTGTGATAGTAATGGCTGATAGTCTCTACTACTCGAAATTCCGGGACTACTGTAAGCCAGGTTTTGACGGGCGCAATTGCATTGCTGATGAACATTACTTGCCAACCTTTTTCAATGTAATTGATCCTGGTGGCATTGCAAACTGGTCTGTGACACATGTTGATTGGTCTGAAAGAAAGTGGCATCCAAAGTTATACAAGTCTCAGGATATTACATATGAGCTTCTGAAGAATATTACGTCAGTTGATGTGAGCGTGCATGTAACCAGTGATGCGAAGAAAGTAGTACAGAGATGGCCTTGCTTATGGAATGGTATACAACGACCGTGTTACTTATTTGCAAGGAAGTTCCATCCAGAGACTCTCAATGACTTGTTGCACCTTTTTGCCAATTATACAACAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

392

Amino Acids

45.44

Weight (kDa)

8.59

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 106 - 335 6.4e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 226
Acc36I ACCTGC 1 cut(s) 226
AccI GTMKAC 1 cut(s) 1084
AciI CCGC 2 cut(s) 68, 146
AclWI GGATC 4 cut(s) 539, 656, 669, 887
AcsI RAATTY 2 cut(s) 495, 806
AcuI CTGAAG 1 cut(s) 1016
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 3 cut(s) 588, 596, 1056
AfeI AGCGCT 1 cut(s) 160
AflIII ACRYGT 3 cut(s) 597, 699, 925
AgsI TTSAA 5 cut(s) 76, 299, 501, 542, 883
AjnI CCWGG 4 cut(s) 302, 664, 826, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 3 cut(s) 361, 642, 991
AluI AGCT 3 cut(s) 361, 642, 991
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 4 cut(s) 109, 797, 975, 1121
AlwI GGATC 4 cut(s) 539, 656, 669, 887
Ama87I CYCGRG 1 cut(s) 50
Aor51HI AGCGCT 1 cut(s) 160
AoxI GGCC 3 cut(s) 53, 80, 1064
ApeKI GCWGC 2 cut(s) 156, 161
ApoI RAATTY 2 cut(s) 495, 806
Asp700I GAANNNNTTC 1 cut(s) 496
AspLEI GCGC 2 cut(s) 161, 843
AspS9I GGNCC 2 cut(s) 53, 668
AsuC2I CCSGG 4 cut(s) 51, 52, 479, 812
AsuHPI GGTGA 2 cut(s) 44, 493
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 668
BamHI GGATCC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 2 cut(s) 148, 168
BccI CCATC 3 cut(s) 69, 1056, 1128
BceAI ACGGC 1 cut(s) 429
BciT130I CCWGG 4 cut(s) 304, 666, 828, 897
BcnI CCSGG 4 cut(s) 51, 52, 479, 812
BcoDI GTCTC 4 cut(s) 109, 797, 975, 1121
BfaI CTAG 2 cut(s) 23, 183
BfoI RGCGCY 1 cut(s) 162
BfuAI ACCTGC 1 cut(s) 226
BglII AGATCT 1 cut(s) 42
BisI GCNGC 2 cut(s) 157, 162
BlsI GCNGC 2 cut(s) 158, 163
Bme1390I CCNGG 8 cut(s) 51, 52, 304, 479, 666, 812, 828, 897
Bme18I GGWCC 1 cut(s) 668
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 53, 668
BmiI GGNNCC 1 cut(s) 663
BmrFI CCNGG 8 cut(s) 51, 52, 304, 479, 666, 812, 828, 897
BmsI GCATC 5 cut(s) 126, 263, 499, 961, 1030
BpiI GAAGAC 1 cut(s) 11
BpuMI CCSGG 4 cut(s) 51, 52, 479, 812
BsaHI GRCGYC 1 cut(s) 19
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 50, 379
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 3 cut(s) 212, 917, 1035
Bse3DI GCAATG 3 cut(s) 344, 849, 903
BseBI CCWGG 4 cut(s) 304, 666, 828, 897
BseDI CCNNGG 2 cut(s) 50, 379
BseGI GGATG 3 cut(s) 254, 952, 1120
BseMI GCAATG 3 cut(s) 344, 849, 903
BseMII CTCAG 1 cut(s) 986
BseNI ACTGG 3 cut(s) 212, 917, 1035
BseXI GCAGC 2 cut(s) 148, 168
Bsh1285I CGRYCG 1 cut(s) 1094
BshFI GGCC 3 cut(s) 55, 82, 1066
BsiEI CGRYCG 1 cut(s) 1094
BsiHKAI GWGCWC 1 cut(s) 742
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 4 cut(s) 51, 83, 479, 811
BslFI GGGAC 1 cut(s) 827
BsmAI GTCTC 4 cut(s) 109, 797, 975, 1121
BsmFI GGGAC 1 cut(s) 827
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 3 cut(s) 55, 82, 1066
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 742
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 5 cut(s) 42, 249, 544, 661, 892
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 2 cut(s) 68, 146
BspANI GGCC 3 cut(s) 55, 82, 1066
BspCNI CTCAG 1 cut(s) 985
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 1 cut(s) 663
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 4 cut(s) 539, 656, 669, 887
BspQI GCTCTTC 1 cut(s) 543
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 3 cut(s) 344, 849, 903
BsrFI RCCGGY 1 cut(s) 82
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 3 cut(s) 212, 917, 1035
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 50, 379
BssMI GATC 5 cut(s) 42, 249, 544, 661, 892
BssNAI GTATAC 1 cut(s) 1085
BssNI GRCGYC 1 cut(s) 19
BssT1I CCWWGG 1 cut(s) 379
Bst1107I GTATAC 1 cut(s) 1085
Bst2UI CCWGG 4 cut(s) 304, 666, 828, 897
Bst4CI ACNGT 3 cut(s) 744, 821, 1095
Bst6I CTCTTC 2 cut(s) 71, 543
BstACI GRCGYC 1 cut(s) 19
BstAPI GCANNNNNTGC 1 cut(s) 46
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 3 cut(s) 68, 768, 1025
BstDEI CTNAG 1 cut(s) 972
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 3 cut(s) 254, 952, 1120
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 2 cut(s) 161, 843
BstKTI GATC 5 cut(s) 45, 252, 547, 664, 895
BstMAI GTCTC 4 cut(s) 109, 797, 975, 1121
BstMBI GATC 5 cut(s) 42, 249, 544, 661, 892
BstMCI CGRYCG 1 cut(s) 1094
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 4 cut(s) 304, 666, 828, 897
BstNSI RCATGY 5 cut(s) 40, 703, 770, 929, 1031
BstSCI CCNGG 8 cut(s) 49, 50, 302, 477, 664, 810, 826, 895
BstV1I GCAGC 2 cut(s) 148, 168
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 3 cut(s) 42, 544, 661
BstYI RGATCY 3 cut(s) 42, 544, 661
BstZ17I GTATAC 1 cut(s) 1085
BsuRI GGCC 3 cut(s) 55, 82, 1066
BtgI CCRYGG 1 cut(s) 379
BtgZI GCGATG 1 cut(s) 47
BtsCI GGATG 3 cut(s) 254, 952, 1120
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 4 cut(s) 20, 478, 749, 1042
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 3 cut(s) 68, 768, 1025
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 161, 843
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 53, 668
Cfr9I CCCGGG 1 cut(s) 50
CseI GACGC 2 cut(s) 8, 749
Csp6I GTAC 3 cut(s) 587, 595, 1055
CviQI GTAC 3 cut(s) 587, 595, 1055
DdeI CTNAG 1 cut(s) 972
DpnI GATC 5 cut(s) 44, 251, 546, 663, 894
DpnII GATC 5 cut(s) 42, 249, 544, 661, 892
Eam1104I CTCTTC 2 cut(s) 71, 543
EarI CTCTTC 2 cut(s) 71, 543
EciI GGCGGA 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 379
Eco31I GGTCTC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 668
Eco47III AGCGCT 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 1016
Eco88I CYCGRG 1 cut(s) 50
EcoRII CCWGG 4 cut(s) 302, 664, 826, 895
EcoT14I CCWWGG 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 379
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 1 cut(s) 827
FauI CCCGC 1 cut(s) 75
FauNDI CATATG 1 cut(s) 985
FblI GTMKAC 1 cut(s) 1084
Fnu4HI GCNGC 2 cut(s) 157, 162
FokI GGATG 3 cut(s) 241, 939, 1107
Fsp4HI GCNGC 2 cut(s) 157, 162
FspBI CTAG 2 cut(s) 23, 183
GlaI GCGC 2 cut(s) 160, 842
GluI GCNGC 2 cut(s) 157, 162
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 3 cut(s) 55, 82, 1066
HapII CCGG 4 cut(s) 51, 83, 479, 811
HgaI GACGC 2 cut(s) 8, 749
HhaI GCGC 2 cut(s) 161, 843
Hin1I GRCGYC 1 cut(s) 19
Hin6I GCGC 2 cut(s) 159, 841
HinP1I GCGC 2 cut(s) 159, 841
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 4 cut(s) 10, 307, 466, 1129
HpaII CCGG 4 cut(s) 51, 83, 479, 811
HphI GGTGA 2 cut(s) 44, 493
Hpy166II GTNNAC 4 cut(s) 587, 597, 649, 1085
Hpy188I TCNGA 6 cut(s) 205, 577, 624, 694, 940, 996
Hpy188III TCNNGA 7 cut(s) 183, 386, 461, 542, 548, 974, 1124
Hpy8I GTNNAC 4 cut(s) 587, 597, 649, 1085
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 383, 387
HpyCH4III ACNGT 3 cut(s) 744, 821, 1095
HpyCH4IV ACGT 2 cut(s) 599, 1007
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 1 cut(s) 972
HpySE526I ACGT 2 cut(s) 599, 1007
Hsp92I GRCGYC 1 cut(s) 19
HspAI GCGC 2 cut(s) 159, 841
Kzo9I GATC 5 cut(s) 42, 249, 544, 661, 892
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 2 cut(s) 226, 737
Lsp1109I GCAGC 2 cut(s) 148, 168
LweI GCATC 5 cut(s) 126, 263, 499, 961, 1030
MaeI CTAG 2 cut(s) 23, 183
MaeII ACGT 2 cut(s) 599, 1007
MaeIII GTNAC 8 cut(s) 32, 167, 446, 473, 702, 919, 1030, 1097
MalI GATC 5 cut(s) 44, 251, 546, 663, 894
MboI GATC 5 cut(s) 42, 249, 544, 661, 892
MboII GAAGA 8 cut(s) 16, 88, 171, 530, 531, 730, 1009, 1057
MfeI CAATTG 1 cut(s) 844
MflI RGATCY 3 cut(s) 42, 544, 661
MhlI GDGCHC 1 cut(s) 742
MlyI GAGTC 1 cut(s) 1123
MnlI CCTC 9 cut(s) 66, 72, 149, 159, 179, 300, 652, 678, 681
MroXI GAANNNNTTC 1 cut(s) 496
MseI TTAA 1 cut(s) 281
MslI CAYNNNNRTG 2 cut(s) 122, 384
MspI CCGG 4 cut(s) 51, 83, 479, 811
MspR9I CCNGG 8 cut(s) 51, 52, 304, 479, 666, 812, 828, 897
MunI CAATTG 1 cut(s) 844
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 4 cut(s) 304, 666, 828, 897
MwoI GCNNNNNNNGC 1 cut(s) 46
NciI CCSGG 4 cut(s) 51, 52, 479, 812
NcoI CCATGG 1 cut(s) 379
NdeI CATATG 1 cut(s) 985
NdeII GATC 5 cut(s) 42, 249, 544, 661, 892
NlaIV GGNNCC 1 cut(s) 663
NmuCI GTSAC 3 cut(s) 32, 473, 919
NspI RCATGY 5 cut(s) 40, 703, 770, 929, 1031
PaeI GCATGC 1 cut(s) 770
PagI TCATGA 1 cut(s) 385
PaqCI CACCTGC 1 cut(s) 226
PciI ACATGT 2 cut(s) 699, 925
PciSI GCTCTTC 1 cut(s) 543
PctI GAATGC 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 496
PfeI GAWTC 3 cut(s) 10, 307, 466
PfoI TCCNGGA 2 cut(s) 302, 810
PkrI GCNGC 2 cut(s) 158, 163
PleI GAGTC 1 cut(s) 1123
PpsI GAGTC 1 cut(s) 1123
PscI ACATGT 2 cut(s) 699, 925
Psp6I CCWGG 4 cut(s) 302, 664, 826, 895
PspGI CCWGG 4 cut(s) 302, 664, 826, 895
PspN4I GGNNCC 1 cut(s) 663
PspPI GGNCC 2 cut(s) 53, 668
PsrI GAACNNNNNNTAC 2 cut(s) 687, 719
PsuI RGATCY 3 cut(s) 42, 544, 661
RsaI GTAC 3 cut(s) 588, 596, 1056
RsaNI GTAC 3 cut(s) 587, 595, 1055
RseI CAYNNNNRTG 2 cut(s) 122, 384
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 2 cut(s) 157, 162
Sau3AI GATC 5 cut(s) 42, 249, 544, 661, 892
Sau96I GGNCC 2 cut(s) 53, 668
SchI GAGTC 1 cut(s) 1123
ScrFI CCNGG 8 cut(s) 51, 52, 304, 479, 666, 812, 828, 897
SduI GDGCHC 1 cut(s) 742
SfaNI GCATC 5 cut(s) 126, 263, 499, 961, 1030
SinI GGWCC 1 cut(s) 668
SmaI CCCGGG 1 cut(s) 52
SmiMI CAYNNNNRTG 2 cut(s) 122, 384
SphI GCATGC 1 cut(s) 770
SrfI GCCCGGGC 1 cut(s) 52
SsiI CCGC 2 cut(s) 68, 146
SspI AATATT 1 cut(s) 1003
SspMI CTAG 2 cut(s) 23, 183
StyD4I CCNGG 8 cut(s) 49, 50, 302, 477, 664, 810, 826, 895
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 3 cut(s) 744, 821, 1095
TaiI ACGT 2 cut(s) 602, 1010
TaqI TCGA 2 cut(s) 460, 803
TatI WGTACW 2 cut(s) 594, 1054
TfiI GAWTC 3 cut(s) 10, 307, 466
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 4 cut(s) 20, 478, 749, 1042
TseFI GTSAC 3 cut(s) 32, 473, 919
TseI GCWGC 2 cut(s) 156, 161
Tsp45I GTSAC 3 cut(s) 32, 473, 919
TspDTI ATGAA 7 cut(s) 17, 313, 402, 425, 641, 770, 873
TspMI CCCGGG 1 cut(s) 50
TspRI CASTG 4 cut(s) 20, 478, 749, 1042
VpaK11BI GGWCC 1 cut(s) 668
XapI RAATTY 2 cut(s) 495, 806
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 5 cut(s) 40, 703, 770, 929, 1031
XmaI CCCGGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 1084
XmnI GAANNNNTTC 1 cut(s) 496
XspI CTAG 2 cut(s) 23, 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.