Rorug04G0021400

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004280
Physical Location & Seq
Reverse (-)
3095279 .. 3098179
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0021400.1

Sequence Viewer

Length: 2901 bp
ATGAGTAGCAGCACTGTAAGTGCAAATCTGAATCTCGTTGAGCTCAACAAGGGGTTCAAAATCGATAACAAATGCCTCACTCAACTCATGAAGGAGAAGAATCTAAACCTCCTTCAAGAGCTCGGAGGCACTGATGGTATTGCGTCAGCTCTTGAAGCCGACAGTGAGCGTGGAGTCCATGATGAAAAGGACAACATTGCTCGCCACCGTGAAGCATTTGGCTCAAACACGTATAGCAAACCACCTTCGAAAAGCCTTTTCCATTTTATGTGGCAAGCCTCTAAGGACCTCACCATTGTCATCCTCCTAGGCTGCGCTGCGCTTTCGCTTGGCTTTGGCATCAAGCAAGATGGACTCAAAGAAGGTTGGTATGATGGTGGAAGCATCTTCATTGCTGTTGTTCTCATCGTTGCTATATCTGCCTTTAGTGACTACAGACAGAACAAGCAGTTCGAAAAGCTGTCCAAAGCAAGTGATGATATCCCAATAGATGTTGTAAGAGGTGGAAGAAGACAGAAGGTTTCCATCTTTGAAATTGTAGTTGGAGATGTTGTGTTTCTGAAGATTGGAGACCAAGTTCCTGCTGATGGATTGTTCTTATATGGACATTCGTTGCGAGTAGACGAATCAAGCATGACAGGGGAGAGTGACCATGTGGAGGTTGATTGCAGCAACAATCCATTCTTGTATTCTGGAACCAAAGTGGTTGATGGATACGGCCGAATGCTTGTGACTTCAGTAGGGATGAACTCAAGTTGGGGCCAAATGATGAGCTTGATCAGCCACGAAAACAATGAGCAGACACCTTTACAAGAAAAGCTTAGTAAGTTCACTCCATTACTTGCTAAGATTGGCTTGGCAGTTGCATTCATGGTCTTTGTGGTGCTGTTAGTTCGTTACTTCACAGGAAACACAAAGAGTGAGAATGGAAGTTTTGGTAAGTTCAAGGGTGGCAAGACGAGGATTGATGATGTGGCCAATGCCTTGTTGGATATGGTAGCAGTTGCTGTCATTATTGTTGTGATTGCAATTCCAGAAGGGTTTCCACTGGCTGTGACTCTAACTCTTGCTTACTCGATGAAGAAAATGATGGCGGATCATGCATTGGTTCGGAGGCTCTCAGCTTGCGAGACTATGGGATGTGCCACCATAATCTGTACAGACAAAACAGGTACTCTCACACTCAACGAGATGAAGGTTGTGAAGTTTTGGATTGGAAAGGAAAGTGTGGAAGATGGTGCTGCTCATTCATCAATTCCTGTTAGTATTCTTCATTTGATCCGAGAAGGGGTAGCGTTCAACACAACAGGAAGTGTTTACAAGCCTAACTCAGGATCAGGGTTTGAGTTTTCCGGCAGTCCTACTGAAAAGGCCATTCTTTCATGGGCTGCAGTTGAGCTGAACATGGACATCGATGAGTTGAAACAGAACTGTACCATTCAACATGTAGAGGCCTTCAATTCACAGAAGAAGCGAAGCGGGGTTTTGGTGGGAAGGAAGGCAGACAATGCAATCCATGTGCATTGGAAAGGAGCTGCGGAGATGATAGTAGACATGTGCTCAAGTTACTACGATGCCTCTGGTGTTACCAGTGAGCTTGGTGAAGATGAAAGGAAGCAGTTCAAGAAAATTGTTCAAGGAATGGCAGCAAGCAGTCTCAGATGCATTGCTTTTGCACACAAACAAGTTCGAGAAAGCAATGATGAAAATGATAGCAAGATGCTGGAAGAGCACAACTTGATCCTATTAGGCCTGGTGGGTTTGAAGGACCCATGTAGGCCAGGAGTGAGAGAGGCAGTACAAGCTTGCCAGTTCGCCGGGGTTCAAGTAAAATTGATCACCGGTGACAATGTGTTTACCGCTACAGCCATAGCTACTGAGTGTGGGATACTGAGGCCTAATGACCAGGAAATGGTAAGGGAAGCAGTGGTAGAAGGTGAAGAATTTCGAAACTACACACCAGAGCAGAGAATGCAGAGAGTTGATGGAATTCGTGTGATGGCAAGGTCTTCTCCATTTGATAAGCTTCTTATGGTGCAGTGCTTAAAACAGAAAGGGCATGTGGTTGCTGTGACCGGTGATGGCACAAATGATGCACCAGCGCTGAAGGAAGCCGATATAGGACTCTCGATGGGGATTCAAGGCACCGAAGTGGCCAAGGAGAGTTCAGACATTGTGATCCTAGATGACAACTTTGCTACTGTTGTCACTGTTTTAAGGTGGGGAAGAGGTGTGTATGCCAACATTCAGAAGTTTGTGCAATTCCAGCTCACAATCAATGTTGCCACTCTTGTAGTCAACTTTGTGGCAGCAGCTTCAGCTGGTGAAGTGCCTTTAACAGCAGTCCAGTTACTGTGGGTGAATCTGATAATGGACACAATGGCTGCTCTTGCTCTTGCCACAGATAAGCCAACCAAAGACCTCATGGAACGGCCACCGGTGCTTCGATCTGAGCCTGTGATCACATACCTCATGTGGAGGAACGTTTTTGCTCAAGCTCTGTTCCAGGTAGCTGTGCTCTTGATCTTGCAGTTCAAAGGCAGATCAATCTTTGGTGTTAGTGAGAAGGTAAACAACACTATGATCTTCAACGTCTTTGTCTTGTGCCAAGTGTTTAATGAATTCAATGCAAGGAGTGTTGAGAAGAAGAATGTCCTCAAAGGGAGTCACAAGAACAAGTTGTTTTGGGCGATCGTTGGTATCACCATTATAGTTCAGGTGGTTATGGTGGAGTTTCTGAAGAAGTTTGCAGATACAGAGAGGTTAAGTTGGGGTCAATGGGGTGCTTGCATTGGAATTTCAGCCATATCTTGCCCAATCAGTTGGGTTGTCAAGTGTATACCAGTTCCTGCGAAGCCGATATTCGCTTATCTAAAGAAGCATAATACAATCACACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

966

Amino Acids

105.94

Weight (kDa)

6.8

Isoelectric Point (pI)

28.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 41 - 109 2e-11 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 164 - 260 1.4e-21 P-type ATPase actuator domain
Hydrolase PF00702 384 - 706 5.9e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 425 - 594 2.8e-30 P-type ATPase, cytoplasmic domain N
Cation_ATPase_C PF00689 777 - 946 4.1e-49 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2144
AccB7I CCANNNNNTGG 1 cut(s) 1912
AccI GTMKAC 3 cut(s) 621, 1551, 2839
AciI CCGC 4 cut(s) 1094, 1479, 1538, 1860
AclI AACGTT 1 cut(s) 2484
AclWI GGATC 5 cut(s) 1104, 1273, 1342, 1735, 2173
AcoI YGGCCR 4 cut(s) 718, 975, 2154, 2432
AcsI RAATTY 4 cut(s) 1943, 1989, 2621, 2796
AcuI CTGAAG 5 cut(s) 581, 720, 2126, 2301, 2759
AfaI GTAC 4 cut(s) 1159, 1174, 1435, 1800
AfeI AGCGCT 1 cut(s) 2103
AfiI CCNNNNNNNGG 5 cut(s) 587, 658, 1288, 1331, 1912
AflIII ACRYGT 3 cut(s) 228, 1444, 1554
AgeI ACCGGT 3 cut(s) 1841, 2075, 2437
AjnI CCWGG 4 cut(s) 1752, 1780, 1905, 2505
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AleI CACNNNNGTG 1 cut(s) 2150
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
Alw21I GWGCWC 5 cut(s) 45, 123, 1562, 1734, 2520
Alw26I GTCTC 3 cut(s) 564, 1124, 1661
AlwI GGATC 5 cut(s) 1104, 1273, 1342, 1735, 2173
AlwNI CAGNNNCTG 3 cut(s) 1007, 2353, 2849
Aor51HI AGCGCT 1 cut(s) 2103
ApoI RAATTY 4 cut(s) 1943, 1989, 2621, 2796
AsiGI ACCGGT 3 cut(s) 1841, 2075, 2437
Asp700I GAANNNNTTC 3 cut(s) 1041, 1619, 1944
AspA2I CCTAGG 1 cut(s) 307
AspLEI GCGC 3 cut(s) 317, 322, 2104
AspS9I GGNCC 3 cut(s) 286, 760, 1768
AsuC2I CCSGG 1 cut(s) 1819
AsuHPI GGTGA 9 cut(s) 283, 1613, 1831, 1856, 1949, 2090, 2336, 2371, 2695
AsuII TTCGAA 3 cut(s) 248, 453, 1948
AvaII GGWCC 2 cut(s) 286, 1768
AvrII CCTAGG 1 cut(s) 307
BalI TGGCCA 2 cut(s) 977, 2156
BanI GGYRCC 1 cut(s) 2144
BanII GRGCYC 2 cut(s) 45, 123
BbsI GAAGAC 2 cut(s) 517, 2001
Bbv12I GWGCWC 5 cut(s) 45, 123, 1562, 1734, 2520
BceAI ACGGC 2 cut(s) 733, 2447
BciT130I CCWGG 4 cut(s) 1754, 1782, 1907, 2507
BciVI GTATCC 2 cut(s) 707, 1881
BclI TGATCA 3 cut(s) 777, 1836, 2460
BcnI CCSGG 1 cut(s) 1819
BcoDI GTCTC 3 cut(s) 564, 1124, 1661
BfaI CTAG 2 cut(s) 308, 2183
BfmI CTRYAG 3 cut(s) 433, 1389, 1863
BfoI RGCGCY 1 cut(s) 2105
BfuI GTATCC 2 cut(s) 707, 1881
BlnI CCTAGG 1 cut(s) 307
Bme1390I CCNGG 5 cut(s) 1754, 1782, 1819, 1907, 2507
Bme18I GGWCC 2 cut(s) 286, 1768
BmgT120I GGNCC 3 cut(s) 286, 760, 1768
BmiI GGNNCC 4 cut(s) 697, 761, 1770, 2146
BmrFI CCNGG 5 cut(s) 1754, 1782, 1819, 1907, 2507
BmsI GCATC 6 cut(s) 348, 393, 1564, 1652, 1710, 2083
BpiI GAAGAC 2 cut(s) 517, 2001
Bpu14I TTCGAA 3 cut(s) 248, 453, 1948
BpuEI CTTGAG 3 cut(s) 736, 1546, 2478
BpuMI CCSGG 1 cut(s) 1819
Bsa29I ATCGAT 2 cut(s) 63, 1413
BsaAI YACGTR 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 3 cut(s) 307, 1818, 2157
BsaWI WCCGGW 3 cut(s) 1841, 2075, 2437
BsaXI ACNNNNNCTCC 4 cut(s) 537, 561, 567, 591
Bsc4I CCNNNNNNNGG 5 cut(s) 587, 658, 1288, 1331, 1912
Bse118I RCCGGY 3 cut(s) 1841, 2075, 2437
Bse1I ACTGG 5 cut(s) 1053, 1590, 1810, 2347, 2843
Bse3DI GCAATG 4 cut(s) 195, 390, 1665, 1705
BseBI CCWGG 4 cut(s) 1754, 1782, 1907, 2507
BseCI ATCGAT 2 cut(s) 63, 1413
BseDI CCNNGG 3 cut(s) 307, 1818, 2157
BseGI GGATG 3 cut(s) 300, 750, 1145
BseLI CCNNNNNNNGG 5 cut(s) 587, 658, 1288, 1331, 1912
BseMI GCAATG 4 cut(s) 195, 390, 1665, 1705
BseMII CTCAG 6 cut(s) 1134, 1344, 1672, 1869, 1883, 2442
BseNI ACTGG 5 cut(s) 1053, 1590, 1810, 2347, 2843
BseX3I CGGCCG 1 cut(s) 718
BsgI GTGCAG 1 cut(s) 2057
Bsh1285I CGRYCG 2 cut(s) 721, 2694
BshNI GGYRCC 1 cut(s) 2144
BshTI ACCGGT 3 cut(s) 1841, 2075, 2437
BshVI ATCGAT 2 cut(s) 63, 1413
BsiEI CGRYCG 2 cut(s) 721, 2694
BsiHKAI GWGCWC 5 cut(s) 45, 123, 1562, 1734, 2520
BsiSI CCGG 5 cut(s) 1353, 1818, 1842, 2076, 2438
BslI CCNNNNNNNGG 5 cut(s) 587, 658, 1288, 1331, 1912
BsmAI GTCTC 3 cut(s) 564, 1124, 1661
BsmI GAATGC 3 cut(s) 729, 866, 1977
Bso31I GGTCTC 1 cut(s) 564
Bsp119I TTCGAA 3 cut(s) 248, 453, 1948
Bsp1286I GDGCHC 5 cut(s) 45, 123, 1562, 1734, 2520
Bsp1407I TGTACA 1 cut(s) 1157
BspACI CCGC 4 cut(s) 1094, 1479, 1538, 1860
BspCNI CTCAG 6 cut(s) 1133, 1343, 1671, 1870, 1884, 2443
BspDI ATCGAT 2 cut(s) 63, 1413
BspHI TCATGA 1 cut(s) 87
BspLI GGNNCC 4 cut(s) 697, 761, 1770, 2146
BspMAI CTGCAG 1 cut(s) 1393
BspPI GGATC 5 cut(s) 1104, 1273, 1342, 1735, 2173
BspQI GCTCTTC 1 cut(s) 1722
BspT104I TTCGAA 3 cut(s) 248, 453, 1948
BspT107I GGYRCC 1 cut(s) 2144
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 4 cut(s) 195, 390, 1665, 1705
BsrFI RCCGGY 3 cut(s) 1841, 2075, 2437
BsrGI TGTACA 1 cut(s) 1157
BsrI ACTGG 5 cut(s) 1053, 1590, 1810, 2347, 2843
BssAI RCCGGY 3 cut(s) 1841, 2075, 2437
BssECI CCNNGG 3 cut(s) 307, 1818, 2157
BssNAI GTATAC 1 cut(s) 2840
BssT1I CCWWGG 2 cut(s) 307, 2157
Bst1107I GTATAC 1 cut(s) 2840
Bst2UI CCWGG 4 cut(s) 1754, 1782, 1907, 2507
Bst4CI ACNGT 7 cut(s) 16, 164, 209, 1433, 2203, 2212, 2355
Bst6I CTCTTC 2 cut(s) 1722, 2221
BstAPI GCANNNNNTGC 2 cut(s) 1508, 1972
BstAUI TGTACA 1 cut(s) 1157
BstBAI YACGTR 1 cut(s) 231
BstBI TTCGAA 3 cut(s) 248, 453, 1948
BstC8I GCNNGC 6 cut(s) 202, 276, 1126, 1651, 1807, 2788
BstDEI CTNAG 9 cut(s) 282, 821, 846, 1120, 1330, 1658, 1878, 1892, 2451
BstENI CCTNNNNNAGG 1 cut(s) 1329
BstF5I GGATG 3 cut(s) 300, 750, 1145
BstH2I RGCGCY 1 cut(s) 2105
BstHHI GCGC 3 cut(s) 317, 322, 2104
BstMAI GTCTC 3 cut(s) 564, 1124, 1661
BstMCI CGRYCG 2 cut(s) 721, 2694
BstNI CCWGG 4 cut(s) 1754, 1782, 1907, 2507
BstNSI RCATGY 3 cut(s) 1448, 1558, 2063
BstSCI CCNGG 5 cut(s) 1752, 1780, 1817, 1905, 2505
BstSFI CTRYAG 3 cut(s) 433, 1389, 1863
BstV2I GAAGAC 2 cut(s) 517, 2001
BstXI CCANNNNNNTGG 1 cut(s) 2823
BstZ17I GTATAC 1 cut(s) 2840
BstZI CGGCCG 1 cut(s) 718
Bsu15I ATCGAT 2 cut(s) 63, 1413
BsuI GTATCC 2 cut(s) 707, 1881
BsuTUI ATCGAT 2 cut(s) 63, 1413
BtsCI GGATG 3 cut(s) 300, 750, 1145
BtsI GCAGTG 2 cut(s) 1932, 2045
BtsIMutI CAGTG 8 cut(s) 12, 129, 169, 1046, 1597, 1932, 2045, 2208
Cac8I GCNNGC 6 cut(s) 202, 276, 1126, 1651, 1807, 2788
CaiI CAGNNNCTG 3 cut(s) 1007, 2353, 2849
CciI TCATGA 1 cut(s) 87
CfoI GCGC 3 cut(s) 317, 322, 2104
Cfr10I RCCGGY 3 cut(s) 1841, 2075, 2437
Cfr13I GGNCC 3 cut(s) 286, 760, 1768
ClaI ATCGAT 2 cut(s) 63, 1413
CseI GACGC 1 cut(s) 132
Csp6I GTAC 4 cut(s) 1158, 1173, 1434, 1799
CspAI ACCGGT 3 cut(s) 1841, 2075, 2437
CviQI GTAC 4 cut(s) 1158, 1173, 1434, 1799
DdeI CTNAG 9 cut(s) 282, 821, 846, 1120, 1330, 1658, 1878, 1892, 2451
EaeI YGGCCR 4 cut(s) 718, 975, 2154, 2432
EagI CGGCCG 1 cut(s) 718
Eam1104I CTCTTC 2 cut(s) 1722, 2221
EarI CTCTTC 2 cut(s) 1722, 2221
EciI GGCGGA 1 cut(s) 1109
Ecl136II GAGCTC 2 cut(s) 43, 121
EclXI CGGCCG 1 cut(s) 718
Eco130I CCWWGG 2 cut(s) 307, 2157
Eco147I AGGCCT 3 cut(s) 1454, 1752, 1897
Eco24I GRGCYC 2 cut(s) 45, 123
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 481
Eco47I GGWCC 2 cut(s) 286, 1768
Eco47III AGCGCT 1 cut(s) 2103
Eco52I CGGCCG 1 cut(s) 718
Eco53kI GAGCTC 2 cut(s) 43, 121
Eco57I CTGAAG 5 cut(s) 581, 720, 2126, 2301, 2759
EcoICRI GAGCTC 2 cut(s) 43, 121
EcoNI CCTNNNNNAGG 1 cut(s) 1329
EcoO109I RGGNCCY 2 cut(s) 286, 1768
EcoRI GAATTC 2 cut(s) 1989, 2621
EcoRII CCWGG 4 cut(s) 1752, 1780, 1905, 2505
EcoRV GATATC 1 cut(s) 481
EcoT14I CCWWGG 2 cut(s) 307, 2157
EcoT22I ATGCAT 2 cut(s) 1105, 1667
EcoT38I GRGCYC 2 cut(s) 45, 123
ErhI CCWWGG 2 cut(s) 307, 2157
FalI AAGNNNNNCTT 4 cut(s) 804, 836, 839, 871
FauI CCCGC 1 cut(s) 1472
FbaI TGATCA 3 cut(s) 777, 1836, 2460
FblI GTMKAC 3 cut(s) 621, 1551, 2839
FokI GGATG 3 cut(s) 287, 757, 1152
FriOI GRGCYC 2 cut(s) 45, 123
FspBI CTAG 2 cut(s) 308, 2183
GlaI GCGC 3 cut(s) 316, 321, 2103
HaeII RGCGCY 1 cut(s) 2105
HapII CCGG 5 cut(s) 1353, 1818, 1842, 2076, 2438
HgaI GACGC 1 cut(s) 132
HhaI GCGC 3 cut(s) 317, 322, 2104
Hin6I GCGC 3 cut(s) 315, 320, 2102
HinP1I GCGC 3 cut(s) 315, 320, 2102
HincII GTYRAC 1 cut(s) 2299
HindII GTYRAC 1 cut(s) 2299
HindIII AAGCTT 3 cut(s) 818, 1803, 2024
HpaII CCGG 5 cut(s) 1353, 1818, 1842, 2076, 2438
HphI GGTGA 9 cut(s) 283, 1613, 1831, 1856, 1949, 2090, 2336, 2371, 2695
Hpy166II GTNNAC 8 cut(s) 622, 831, 1318, 1552, 1857, 2299, 2572, 2840
Hpy8I GTNNAC 8 cut(s) 622, 831, 1318, 1552, 1857, 2299, 2572, 2840
HpyCH4III ACNGT 7 cut(s) 16, 164, 209, 1433, 2203, 2212, 2355
HpyCH4IV ACGT 3 cut(s) 230, 2484, 2592
HpyF3I CTNAG 9 cut(s) 282, 821, 846, 1120, 1330, 1658, 1878, 1892, 2451
HpySE526I ACGT 3 cut(s) 230, 2484, 2592
HspAI GCGC 3 cut(s) 315, 320, 2102
Ksp22I TGATCA 3 cut(s) 777, 1836, 2460
LguI GCTCTTC 1 cut(s) 1722
LmnI GCTCC 1 cut(s) 1532
LweI GCATC 6 cut(s) 348, 393, 1564, 1652, 1710, 2083
MaeI CTAG 2 cut(s) 308, 2183
MaeII ACGT 3 cut(s) 230, 2484, 2592
MhlI GDGCHC 5 cut(s) 45, 123, 1562, 1734, 2520
MlsI TGGCCA 2 cut(s) 977, 2156
MluNI TGGCCA 2 cut(s) 977, 2156
MlyI GAGTC 5 cut(s) 183, 348, 1051, 2118, 2674
MmeI TCCRAC 2 cut(s) 523, 969
Mox20I TGGCCA 2 cut(s) 977, 2156
Mph1103I ATGCAT 2 cut(s) 1105, 1667
MroXI GAANNNNTTC 3 cut(s) 1041, 1619, 1944
MscI TGGCCA 2 cut(s) 977, 2156
MseI TTAA 5 cut(s) 2045, 2216, 2336, 2616, 2765
MslI CAYNNNNRTG 1 cut(s) 2150
Msp20I TGGCCA 2 cut(s) 977, 2156
MspA1I CMGCKG 1 cut(s) 2321
MspI CCGG 5 cut(s) 1353, 1818, 1842, 2076, 2438
MspR9I CCNGG 5 cut(s) 1754, 1782, 1819, 1907, 2507
MteI GCGCNGCGC 1 cut(s) 318
Mva1269I GAATGC 3 cut(s) 729, 866, 1977
MvaI CCWGG 4 cut(s) 1754, 1782, 1907, 2507
NciI CCSGG 1 cut(s) 1819
NlaIV GGNNCC 4 cut(s) 697, 761, 1770, 2146
NmuCI GTSAC 8 cut(s) 428, 647, 730, 1054, 1844, 2071, 2206, 2666
NsiI ATGCAT 2 cut(s) 1105, 1667
NspI RCATGY 3 cut(s) 1448, 1558, 2063
NspV TTCGAA 3 cut(s) 248, 453, 1948
OliI CACNNNNGTG 1 cut(s) 2150
PagI TCATGA 1 cut(s) 87
PceI AGGCCT 3 cut(s) 1454, 1752, 1897
PciI ACATGT 2 cut(s) 1444, 1554
PciSI GCTCTTC 1 cut(s) 1722
PctI GAATGC 3 cut(s) 729, 866, 1977
PdmI GAANNNNTTC 3 cut(s) 1041, 1619, 1944
PfeI GAWTC 5 cut(s) 31, 100, 626, 2137, 2362
PflMI CCANNNNNTGG 1 cut(s) 1912
PinAI ACCGGT 3 cut(s) 1841, 2075, 2437
Ple19I CGATCG 1 cut(s) 2694
PleI GAGTC 5 cut(s) 182, 348, 1051, 2118, 2673
PpsI GAGTC 5 cut(s) 182, 348, 1051, 2118, 2673
Ppu21I YACGTR 1 cut(s) 231
PpuMI RGGWCCY 2 cut(s) 286, 1768
PscI ACATGT 2 cut(s) 1444, 1554
Psp124BI GAGCTC 2 cut(s) 45, 123
Psp1406I AACGTT 1 cut(s) 2484
Psp5II RGGWCCY 2 cut(s) 286, 1768
Psp6I CCWGG 4 cut(s) 1752, 1780, 1905, 2505
PspGI CCWGG 4 cut(s) 1752, 1780, 1905, 2505
PspN4I GGNNCC 4 cut(s) 697, 761, 1770, 2146
PspPI GGNCC 3 cut(s) 286, 760, 1768
PspPPI RGGWCCY 2 cut(s) 286, 1768
PstI CTGCAG 1 cut(s) 1393
PstNI CAGNNNCTG 3 cut(s) 1007, 2353, 2849
PvuI CGATCG 1 cut(s) 2694
PvuII CAGCTG 1 cut(s) 2321
RsaI GTAC 4 cut(s) 1159, 1174, 1435, 1800
RsaNI GTAC 4 cut(s) 1158, 1173, 1434, 1799
RseI CAYNNNNRTG 1 cut(s) 2150
SacI GAGCTC 2 cut(s) 45, 123
SapI GCTCTTC 1 cut(s) 1722
SaqAI TTAA 5 cut(s) 2045, 2216, 2336, 2616, 2765
Sau96I GGNCC 3 cut(s) 286, 760, 1768
SchI GAGTC 5 cut(s) 183, 348, 1051, 2118, 2674
ScrFI CCNGG 5 cut(s) 1754, 1782, 1819, 1907, 2507
SduI GDGCHC 5 cut(s) 45, 123, 1562, 1734, 2520
SfaNI GCATC 6 cut(s) 348, 393, 1564, 1652, 1710, 2083
SfcI CTRYAG 3 cut(s) 433, 1389, 1863
SfuI TTCGAA 3 cut(s) 248, 453, 1948
SgrAI CRCCGGYG 2 cut(s) 1841, 2437
SinI GGWCC 2 cut(s) 286, 1768
SmiMI CAYNNNNRTG 1 cut(s) 2150
SmlI CTYRAG 3 cut(s) 751, 1561, 2493
SmoI CTYRAG 3 cut(s) 751, 1561, 2493
SseBI AGGCCT 3 cut(s) 1454, 1752, 1897
SsiI CCGC 4 cut(s) 1094, 1479, 1538, 1860
SspMI CTAG 2 cut(s) 308, 2183
SstI GAGCTC 2 cut(s) 45, 123
StuI AGGCCT 3 cut(s) 1454, 1752, 1897
StyD4I CCNGG 5 cut(s) 1752, 1780, 1817, 1905, 2505
StyI CCWWGG 2 cut(s) 307, 2157
TaaI ACNGT 7 cut(s) 16, 164, 209, 1433, 2203, 2212, 2355
TaiI ACGT 3 cut(s) 233, 2487, 2595
TaqI TCGA 9 cut(s) 63, 248, 453, 1076, 1413, 1690, 1948, 2129, 2446
TatI WGTACW 2 cut(s) 1157, 1798
TfiI GAWTC 5 cut(s) 31, 100, 626, 2137, 2362
Tru1I TTAA 5 cut(s) 2045, 2216, 2336, 2616, 2765
Tru9I TTAA 5 cut(s) 2045, 2216, 2336, 2616, 2765
TscAI CASTG 8 cut(s) 19, 136, 169, 1053, 1597, 1932, 2045, 2215
TseFI GTSAC 8 cut(s) 428, 647, 730, 1054, 1844, 2071, 2206, 2666
Tsp45I GTSAC 8 cut(s) 428, 647, 730, 1054, 1844, 2071, 2206, 2666
TspRI CASTG 8 cut(s) 19, 136, 169, 1053, 1597, 1932, 2045, 2215
Van91I CCANNNNNTGG 1 cut(s) 1912
VpaK11BI GGWCC 2 cut(s) 286, 1768
XagI CCTNNNNNAGG 1 cut(s) 1329
XapI RAATTY 4 cut(s) 1943, 1989, 2621, 2796
XceI RCATGY 3 cut(s) 1448, 1558, 2063
XcmI CCANNNNNNNNNTGG 2 cut(s) 985, 2422
XmaJI CCTAGG 1 cut(s) 307
XmiI GTMKAC 3 cut(s) 621, 1551, 2839
XmnI GAANNNNTTC 3 cut(s) 1041, 1619, 1944
XspI CTAG 2 cut(s) 308, 2183
Zsp2I ATGCAT 2 cut(s) 1105, 1667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.