Rh4DG092200

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004280
Physical Location & Seq
Reverse (-)
13890576 .. 13894217
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG092200.1

Sequence Viewer

Length: 951 bp
ATGAAGACGGGTCAGCCGTGGCGAATAGGCATGGGTGACATTCCAATATTTTCTGGGGCTCGCCATCGCCCACCTTTGAAGAGGCCGTTGTGGATCATTGTCTTGGTGTCTTTGGTTAGCATCTTTGTGATCTGTGCTTTTCTCTATCAACCGCAAAGCAACGCCGCTTGTTACATATTTTCTTCAAGAGGTTGTAAGGCTATTTCCGATTGGCTTCCACCTGCTCCAGCAAGGGAATATAATGATGATGAGATTGCAGCCCGGGTAGTGATAAAAGAAATTTTGAGTGCACCTCCTGTTCAATCAAAAAACCCGAAAATAGCATTCATGTTCCTTACACCAAGTGCAGTGCCTTTTGAGAAGCTGTGGGATAAATTTTTTCATGGTCATGAGGGAAAGTTCTCTGTTTATGTGCATGCATCTAAAGAAAAGGCAGTTCATTTGAGCCGTTACTTTGCTAATAGAGAGATACACAGTGATCAGGTGATTTGGGGAAAAATTTCCATGGTAGATGCTGAGAGACGATTATTGGCAAATGCTCTTCAAGATCCTGATAACCAGCACTTTGTGCTACTTTCTGAAAGTTGTGTACCGTTGTATACGTTTGACTATATCTATGACTATCTGATGAATACAAATATAAGCTATGTTGACTGCTTTGAGGATCCTGGCCCACATGGAAATGGCAGGTACTCAGAACACATGTTACCTGAAATTGAGAAGAAAGACTTTAGAAAGGGTGCACAGTGGTTCTCTGTTAAGCGTCAGCATGCTGTTATAATAATGGCTGATAGTCTTTACTACTCAAAATTCCGGGATTACTGCAAGCCAGGTTTGGAGGGGCGTAATTGCATTGCTGATGAACATTACTTGCCAACCTTTTTCAATGTGAGGATCTTTGCTCCAACCTTTTCATCTTCAATGTTGCCTTGTTTTAGCGTATCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

316

Amino Acids

36.31

Weight (kDa)

8.33

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 107 - 298 3.1e-69 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 779
AarI CACCTGC 1 cut(s) 229
Acc36I ACCTGC 2 cut(s) 229, 678
AccI GTMKAC 1 cut(s) 599
AciI CCGC 2 cut(s) 152, 165
AclWI GGATC 5 cut(s) 101, 542, 659, 672, 902
AcsI RAATTY 4 cut(s) 279, 374, 498, 809
AdeI CACNNNGTG 2 cut(s) 344, 568
AfaI GTAC 2 cut(s) 591, 692
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 6 cut(s) 79, 186, 302, 545, 886, 921
AjnI CCWGG 2 cut(s) 667, 829
AluBI AGCT 2 cut(s) 364, 645
AluI AGCT 2 cut(s) 364, 645
Alw21I GWGCWC 2 cut(s) 292, 745
Alw26I GTCTC 1 cut(s) 514
Alw44I GTGCAC 2 cut(s) 288, 741
AlwI GGATC 5 cut(s) 101, 542, 659, 672, 902
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 2 cut(s) 83, 670
ApaLI GTGCAC 2 cut(s) 288, 741
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 4 cut(s) 279, 374, 498, 809
Asp700I GAANNNNTTC 1 cut(s) 499
AspS9I GGNCC 1 cut(s) 671
AsuC2I CCSGG 3 cut(s) 262, 263, 815
AsuHPI GGTGA 2 cut(s) 47, 496
AvaI CYCGRG 1 cut(s) 261
BaeGI GKGCMC 2 cut(s) 292, 745
BamHI GGATCC 1 cut(s) 664
BanII GRGCYC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 292, 745
BbvI GCAGC 1 cut(s) 269
BccI CCATC 1 cut(s) 72
BceAI ACGGC 2 cut(s) 70, 432
BciT130I CCWGG 2 cut(s) 669, 831
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 3 cut(s) 262, 263, 815
BcoDI GTCTC 1 cut(s) 514
BfuAI ACCTGC 2 cut(s) 229, 678
BisI GCNGC 2 cut(s) 165, 258
BlsI GCNGC 2 cut(s) 166, 259
Bme1390I CCNGG 5 cut(s) 262, 263, 669, 815, 831
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 1 cut(s) 666
BmrFI CCNGG 5 cut(s) 262, 263, 669, 815, 831
BmsI GCATC 3 cut(s) 129, 428, 502
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 1 cut(s) 210
BpuMI CCSGG 3 cut(s) 262, 263, 815
BsaJI CCNNGG 3 cut(s) 17, 261, 504
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 2 cut(s) 669, 831
BseDI CCNNGG 3 cut(s) 17, 261, 504
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 2 cut(s) 507, 708
BseSI GKGCMC 2 cut(s) 292, 745
BseXI GCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 366
BshFI GGCC 2 cut(s) 85, 672
BsiHKAI GWGCWC 2 cut(s) 292, 745
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 262, 814
BsmAI GTCTC 1 cut(s) 514
BsmBI CGTCTC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 2 cut(s) 85, 672
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 61, 292, 745
Bsp143I GATC 6 cut(s) 93, 129, 478, 547, 664, 894
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 2 cut(s) 152, 165
BspANI GGCC 2 cut(s) 85, 672
BspCNI CTCAG 2 cut(s) 508, 707
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 666
BspMI ACCTGC 2 cut(s) 229, 678
BspPI GGATC 5 cut(s) 101, 542, 659, 672, 902
BspQI GCTCTTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 852
BssECI CCNNGG 3 cut(s) 17, 261, 504
BssMI GATC 6 cut(s) 93, 129, 478, 547, 664, 894
BssNAI GTATAC 1 cut(s) 600
BssT1I CCWWGG 1 cut(s) 504
Bst1107I GTATAC 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 669, 831
Bst4CI ACNGT 3 cut(s) 476, 594, 747
Bst6I CTCTTC 2 cut(s) 74, 546
BstAPI GCANNNNNTGC 1 cut(s) 568
BstC8I GCNNGC 4 cut(s) 61, 417, 771, 827
BstDEI CTNAG 2 cut(s) 516, 694
BstDSI CCRYGG 2 cut(s) 17, 504
BstKTI GATC 6 cut(s) 96, 132, 481, 550, 667, 897
BstMAI GTCTC 1 cut(s) 514
BstMBI GATC 6 cut(s) 93, 129, 478, 547, 664, 894
BstMWI GCNNNNNNNGC 1 cut(s) 568
BstNI CCWGG 2 cut(s) 669, 831
BstNSI RCATGY 3 cut(s) 419, 706, 773
BstSCI CCNGG 5 cut(s) 260, 261, 667, 813, 829
BstSLI GKGCMC 2 cut(s) 292, 745
BstV1I GCAGC 1 cut(s) 269
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 3 cut(s) 547, 664, 894
BstYI RGATCY 3 cut(s) 547, 664, 894
BstZ17I GTATAC 1 cut(s) 600
BsuRI GGCC 2 cut(s) 85, 672
BtgI CCRYGG 2 cut(s) 17, 504
BtgZI GCGATG 1 cut(s) 50
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 3 cut(s) 354, 481, 752
BveI ACCTGC 2 cut(s) 229, 678
Cac8I GCNNGC 4 cut(s) 61, 417, 771, 827
CciI TCATGA 1 cut(s) 388
Cfr13I GGNCC 1 cut(s) 671
Cfr9I CCCGGG 1 cut(s) 261
CseI GACGC 1 cut(s) 752
Csp6I GTAC 2 cut(s) 590, 691
CviAII CATG 9 cut(s) 31, 328, 383, 389, 416, 505, 677, 703, 770
CviQI GTAC 2 cut(s) 590, 691
DdeI CTNAG 2 cut(s) 516, 694
DpnI GATC 6 cut(s) 95, 131, 480, 549, 666, 896
DpnII GATC 6 cut(s) 93, 129, 478, 547, 664, 894
DraIII CACNNNGTG 2 cut(s) 344, 568
Eam1104I CTCTTC 2 cut(s) 74, 546
EarI CTCTTC 2 cut(s) 74, 546
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 667, 829
EcoT14I CCWWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 9 cut(s) 34, 331, 386, 392, 419, 508, 680, 706, 773
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FatI CATG 9 cut(s) 30, 327, 382, 388, 415, 504, 676, 702, 769
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 2 cut(s) 165, 258
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 165, 258
GluI GCNGC 2 cut(s) 165, 258
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 2 cut(s) 85, 672
HapII CCGG 2 cut(s) 262, 814
HgaI GACGC 1 cut(s) 752
Hin1II CATG 9 cut(s) 34, 331, 386, 392, 419, 508, 680, 706, 773
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HpaII CCGG 2 cut(s) 262, 814
HphI GGTGA 2 cut(s) 47, 496
Hpy166II GTNNAC 5 cut(s) 290, 590, 600, 652, 743
Hpy188I TCNGA 5 cut(s) 208, 580, 627, 697, 950
Hpy188III TCNNGA 4 cut(s) 186, 389, 545, 551
Hpy8I GTNNAC 5 cut(s) 290, 590, 600, 652, 743
HpyCH4III ACNGT 3 cut(s) 476, 594, 747
HpyCH4IV ACGT 1 cut(s) 602
HpyCH4V TGCA 8 cut(s) 257, 290, 347, 415, 419, 743, 825, 852
HpyF10VI GCNNNNNNNGC 1 cut(s) 568
HpyF3I CTNAG 2 cut(s) 516, 694
HpySE526I ACGT 1 cut(s) 602
Hsp92II CATG 9 cut(s) 34, 331, 386, 392, 419, 508, 680, 706, 773
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 93, 129, 478, 547, 664, 894
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 2 cut(s) 229, 907
Lsp1109I GCAGC 1 cut(s) 269
LweI GCATC 3 cut(s) 129, 428, 502
MaeII ACGT 1 cut(s) 602
MaeIII GTNAC 4 cut(s) 35, 170, 449, 705
MalI GATC 6 cut(s) 95, 131, 480, 549, 666, 896
MboI GATC 6 cut(s) 93, 129, 478, 547, 664, 894
MboII GAAGA 6 cut(s) 16, 91, 174, 533, 733, 909
MflI RGATCY 3 cut(s) 547, 664, 894
MhlI GDGCHC 3 cut(s) 61, 292, 745
MluCI AATT 6 cut(s) 279, 374, 498, 714, 809, 847
MmeI TCCRAC 1 cut(s) 929
MnlI CCTC 7 cut(s) 75, 182, 303, 385, 655, 832, 885
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 1 cut(s) 759
MslI CAYNNNNRTG 3 cut(s) 125, 387, 681
MspI CCGG 2 cut(s) 262, 814
MspR9I CCNGG 5 cut(s) 262, 263, 669, 815, 831
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 2 cut(s) 669, 831
MwoI GCNNNNNNNGC 1 cut(s) 568
NciI CCSGG 3 cut(s) 262, 263, 815
NcoI CCATGG 1 cut(s) 504
NdeII GATC 6 cut(s) 93, 129, 478, 547, 664, 894
NlaIII CATG 9 cut(s) 34, 331, 386, 392, 419, 508, 680, 706, 773
NlaIV GGNNCC 1 cut(s) 666
NmuCI GTSAC 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 3 cut(s) 419, 706, 773
PaeI GCATGC 2 cut(s) 419, 773
PagI TCATGA 1 cut(s) 388
PaqCI CACCTGC 1 cut(s) 229
PciI ACATGT 1 cut(s) 702
PciSI GCTCTTC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 499
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 2 cut(s) 166, 259
PscI ACATGT 1 cut(s) 702
PsiI TTATAA 1 cut(s) 779
Psp6I CCWGG 2 cut(s) 667, 829
PspGI CCWGG 2 cut(s) 667, 829
PspN4I GGNNCC 1 cut(s) 666
PspPI GGNCC 1 cut(s) 671
PsrI GAACNNNNNNTAC 2 cut(s) 690, 722
PsuI RGATCY 3 cut(s) 547, 664, 894
RsaI GTAC 2 cut(s) 591, 692
RsaNI GTAC 2 cut(s) 590, 691
RseI CAYNNNNRTG 3 cut(s) 125, 387, 681
SapI GCTCTTC 1 cut(s) 546
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 2 cut(s) 165, 258
Sau3AI GATC 6 cut(s) 93, 129, 478, 547, 664, 894
Sau96I GGNCC 1 cut(s) 671
ScrFI CCNGG 5 cut(s) 262, 263, 669, 815, 831
SduI GDGCHC 3 cut(s) 61, 292, 745
SfaNI GCATC 3 cut(s) 129, 428, 502
SmaI CCCGGG 1 cut(s) 263
SmiMI CAYNNNNRTG 3 cut(s) 125, 387, 681
SphI GCATGC 2 cut(s) 419, 773
Sse9I AATT 6 cut(s) 279, 374, 498, 714, 809, 847
SsiI CCGC 2 cut(s) 152, 165
SspI AATATT 1 cut(s) 48
StyD4I CCNGG 5 cut(s) 260, 261, 667, 813, 829
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 3 cut(s) 476, 594, 747
TaiI ACGT 1 cut(s) 605
TasI AATT 6 cut(s) 279, 374, 498, 714, 809, 847
TauI GCSGC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 3 cut(s) 354, 481, 752
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 7 cut(s) 17, 316, 371, 428, 644, 876, 903
TspMI CCCGGG 1 cut(s) 261
TspRI CASTG 3 cut(s) 354, 481, 752
VneI GTGCAC 2 cut(s) 288, 741
XapI RAATTY 4 cut(s) 279, 374, 498, 809
XceI RCATGY 3 cut(s) 419, 706, 773
XmaI CCCGGG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 599
XmnI GAANNNNTTC 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.