AT4G30060

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004280
Physical Location & Seq
Reverse (-)
14688991 .. 14692553
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30060.2

Sequence Viewer

Length: 1206 bp
ATGAAGGCAGTTAAAAGATGGAGTATAGGGAACTTAGCGGACATTCCCGTGTCTTTGCCTGGAGCTCGATACCGTGCTCCTCCTCCAGGAAGAAGACGAGTATGGATCATCATGGTGCTTTCACTGATCACCATGTTCTTTATTATGGCTTACATGTATCCTCATCATAGTAAACGTGCTTGTTATATGATTTCATCAAGAGGATGCAAGGCTTTAGCTGATTGGCTTCCACCTTCTCTGAGGGAATATTCAGATGATGAGATTGCAGCTCGTGTAGTCATTAGAGAAATATTGAGTTCACCTCCTGTTATTAGAAAGAACTCTAAAATTGCTTTCATGTTTTTAACTCCTGGTACATTGCCTTTTGAGAGGCTTTGGGACAGATTTTTCCTGGGCCATGAAGGGAAATTCTCTGTTTATATACATGCATCAAAGGAAAGGCCAGTTCACTACAGTCGTTACTTTCTCAACCGCGAAATTCGTAGTGATGAGGTGGTGTGGGGAAGGATATCAATGGTTGACGCAGAGAGACGGTTGTTAGCTAATGCTCTTAGAGACACTTCTAACCAGCAATTTGTTTTACTCTCTGATAGTTGTGTACCACTTCGAAGTTTTGAATACATTTACAATTACCTGATGCACAGCAATCTCAGCTATGTTGACTGCTTTGACGATCCAGGTCAACATGGAGCAGGCAGGCATATGAATCACATGTTGCCTGAAATCCCAAAGAAGGATTTTCGAAAGGGTGCACAGTGGTTCACCATGAAGCGACAACACGCTGTAGCAACTATGGCAGACAGTCTTTACTACTCTAAATTCCGAGATTACTGCGGGCCAGGTATAGAGAACAACAAGAACTGCATAGCTGATGAACACTATCTGCCAACATTCTTTCATATGCTTGATCCCGGTGGCATTGCTAACTGGACTGTAACACAAGTTGATTGGTCTGAGAGAAAGTGGCATCCCAAAACATATATGCCTGAGGATATCACACACGAGTTACTAAACAACCTCACGTCTACTGACACACTCGTACATGTCACAAGTGTTGGGATGGGTGAAGAAATATGGATGCCTTGTATGTGGAACGGAATCCAAAGACCGTGTTACTTGTTTGGAAGGAAATTTCACCCGGATACACTCGATAAACTGTTGGATCTCTTCTCAAATTACACAAGATCAGTCACTTGGAATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

401

Amino Acids

46.86

Weight (kDa)

8.97

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 110 - 340 5.2e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1025
AccII CGCG 1 cut(s) 474
AciI CCGC 3 cut(s) 38, 472, 834
AclWI GGATC 4 cut(s) 113, 668, 902, 1170
AcsI RAATTY 5 cut(s) 407, 477, 818, 1130, 1198
AfaI GTAC 3 cut(s) 355, 600, 1041
AfiI CCNNNNNNNGG 2 cut(s) 86, 733
AflIII ACRYGT 3 cut(s) 153, 711, 1042
AgsI TTSAA 1 cut(s) 617
AjiI CACGTC 1 cut(s) 1023
AjnI CCWGG 6 cut(s) 58, 85, 349, 390, 676, 838
AluBI AGCT 6 cut(s) 65, 218, 269, 542, 654, 869
AluI AGCT 6 cut(s) 65, 218, 269, 542, 654, 869
Alw21I GWGCWC 3 cut(s) 67, 79, 754
Alw26I GTCTC 2 cut(s) 523, 549
Alw44I GTGCAC 1 cut(s) 750
AlwI GGATC 4 cut(s) 113, 668, 902, 1170
AoxI GGCC 3 cut(s) 394, 440, 836
ApaLI GTGCAC 1 cut(s) 750
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 5 cut(s) 407, 477, 818, 1130, 1198
AspS9I GGNCC 2 cut(s) 394, 836
AsuC2I CCSGG 2 cut(s) 912, 1139
AsuHPI GGTGA 5 cut(s) 121, 291, 754, 1076, 1127
AsuII TTCGAA 2 cut(s) 607, 742
AxyI CCTNAGG 1 cut(s) 987
BaeGI GKGCMC 1 cut(s) 754
BanII GRGCYC 1 cut(s) 67
BauI CACGAG 2 cut(s) 270, 1001
BbsI GAAGAC 1 cut(s) 100
Bbv12I GWGCWC 3 cut(s) 67, 79, 754
BbvI GCAGC 1 cut(s) 278
BccI CCATC 2 cut(s) 12, 1054
BcgI CGANNNNNNTGC 2 cut(s) 813, 847
BciT130I CCWGG 6 cut(s) 60, 87, 351, 392, 678, 840
BciVI GTATCC 2 cut(s) 168, 1135
BclI TGATCA 1 cut(s) 126
BcnI CCSGG 2 cut(s) 912, 1139
BcoDI GTCTC 2 cut(s) 523, 549
BfmI CTRYAG 2 cut(s) 451, 783
BfuI GTATCC 2 cut(s) 168, 1135
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 8 cut(s) 60, 87, 351, 392, 678, 840, 912, 1139
BmgBI CACGTC 1 cut(s) 1023
BmgT120I GGNCC 2 cut(s) 394, 836
BmrFI CCNGG 8 cut(s) 60, 87, 351, 392, 678, 840, 912, 1139
BmsI GCATC 5 cut(s) 194, 437, 627, 976, 1068
BpiI GAAGAC 1 cut(s) 100
BplI GAGNNNNNCTC 2 cut(s) 286, 318
BpmI CTGGAG 2 cut(s) 69, 81
Bpu14I TTCGAA 2 cut(s) 607, 742
BpuMI CCSGG 2 cut(s) 912, 1139
BsaJI CCNNGG 1 cut(s) 391
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 733
Bse1I ACTGG 2 cut(s) 443, 932
Bse21I CCTNAGG 1 cut(s) 987
Bse3DI GCAATG 2 cut(s) 356, 918
BseBI CCWGG 6 cut(s) 60, 87, 351, 392, 678, 840
BseDI CCNNGG 1 cut(s) 391
BseGI GGATG 4 cut(s) 209, 967, 1065, 1083
BseLI CCNNNNNNNGG 2 cut(s) 86, 733
BseMI GCAATG 2 cut(s) 356, 918
BseMII CTCAG 4 cut(s) 230, 664, 945, 978
BseNI ACTGG 2 cut(s) 443, 932
BseRI GAGGAG 2 cut(s) 69, 72
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 3 cut(s) 396, 442, 838
BsiHKAI GWGCWC 3 cut(s) 67, 79, 754
BsiSI CCGG 2 cut(s) 912, 1139
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 2 cut(s) 86, 733
BsmAI GTCTC 2 cut(s) 523, 549
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 392
BsnI GGCC 3 cut(s) 396, 442, 838
Bsp119I TTCGAA 2 cut(s) 607, 742
Bsp1286I GDGCHC 3 cut(s) 67, 79, 754
Bsp143I GATC 6 cut(s) 105, 126, 673, 907, 1162, 1184
BspACI CCGC 3 cut(s) 38, 472, 834
BspANI GGCC 3 cut(s) 396, 442, 838
BspCNI CTCAG 4 cut(s) 231, 663, 946, 979
BspFNI CGCG 1 cut(s) 474
BspPI GGATC 4 cut(s) 113, 668, 902, 1170
BspT104I TTCGAA 2 cut(s) 607, 742
BsrDI GCAATG 2 cut(s) 356, 918
BsrI ACTGG 2 cut(s) 443, 932
BssECI CCNNGG 1 cut(s) 391
BssMI GATC 6 cut(s) 105, 126, 673, 907, 1162, 1184
BssSI CACGAG 2 cut(s) 270, 1001
Bst2BI CACGAG 2 cut(s) 270, 1001
Bst2UI CCWGG 6 cut(s) 60, 87, 351, 392, 678, 840
Bst4CI ACNGT 8 cut(s) 74, 455, 534, 756, 803, 934, 1110, 1158
Bst6I CTCTTC 1 cut(s) 1172
BstBI TTCGAA 2 cut(s) 607, 742
BstC8I GCNNGC 3 cut(s) 694, 698, 836
BstDEI CTNAG 6 cut(s) 34, 239, 551, 650, 954, 987
BstENI CCTNNNNNAGG 1 cut(s) 84
BstF5I GGATG 4 cut(s) 209, 967, 1065, 1083
BstFNI CGCG 1 cut(s) 474
BstKTI GATC 6 cut(s) 108, 129, 676, 910, 1165, 1187
BstMAI GTCTC 2 cut(s) 523, 549
BstMBI GATC 6 cut(s) 105, 126, 673, 907, 1162, 1184
BstMWI GCNNNNNNNGC 2 cut(s) 651, 794
BstNI CCWGG 6 cut(s) 60, 87, 351, 392, 678, 840
BstNSI RCATGY 4 cut(s) 157, 428, 715, 1046
BstSCI CCNGG 8 cut(s) 58, 85, 349, 390, 676, 838, 910, 1137
BstSFI CTRYAG 2 cut(s) 451, 783
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 100
BstX2I RGATCY 1 cut(s) 1162
BstYI RGATCY 1 cut(s) 1162
Bsu36I CCTNAGG 1 cut(s) 987
BsuI GTATCC 2 cut(s) 168, 1135
BsuRI GGCC 3 cut(s) 396, 442, 838
BtrI CACGTC 1 cut(s) 1023
BtsCI GGATG 4 cut(s) 209, 967, 1065, 1083
BtsIMutI CAGTG 2 cut(s) 122, 761
Cac8I GCNNGC 3 cut(s) 694, 698, 836
Cfr13I GGNCC 2 cut(s) 394, 836
CseI GACGC 1 cut(s) 530
Csp6I GTAC 3 cut(s) 354, 599, 1040
CviQI GTAC 3 cut(s) 354, 599, 1040
DdeI CTNAG 6 cut(s) 34, 239, 551, 650, 954, 987
DpnI GATC 6 cut(s) 107, 128, 675, 909, 1164, 1186
DpnII GATC 6 cut(s) 105, 126, 673, 907, 1162, 1184
Eam1104I CTCTTC 1 cut(s) 1172
EarI CTCTTC 1 cut(s) 1172
Ecl136II GAGCTC 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 67
Eco32I GATATC 2 cut(s) 510, 994
Eco53kI GAGCTC 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 987
EcoICRI GAGCTC 1 cut(s) 65
EcoNI CCTNNNNNAGG 1 cut(s) 84
EcoRII CCWGG 6 cut(s) 58, 85, 349, 390, 676, 838
EcoRV GATATC 2 cut(s) 510, 994
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 523
FaqI GGGAC 1 cut(s) 392
FauI CCCGC 1 cut(s) 827
FauNDI CATATG 2 cut(s) 702, 900
FbaI TGATCA 1 cut(s) 126
FblI GTMKAC 1 cut(s) 1025
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 4 cut(s) 216, 954, 1072, 1090
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 267
GluI GCNGC 1 cut(s) 267
GsuI CTGGAG 2 cut(s) 69, 81
HaeIII GGCC 3 cut(s) 396, 442, 838
HapII CCGG 2 cut(s) 912, 1139
HgaI GACGC 1 cut(s) 530
HincII GTYRAC 3 cut(s) 520, 661, 683
HindII GTYRAC 3 cut(s) 520, 661, 683
HinfI GANTC 2 cut(s) 706, 1098
HpaII CCGG 2 cut(s) 912, 1139
HphI GGTGA 5 cut(s) 121, 291, 754, 1076, 1127
Hpy188I TCNGA 5 cut(s) 240, 253, 589, 824, 955
Hpy188III TCNNGA 1 cut(s) 198
HpyAV CCTTC 5 cut(s) 243, 395, 498, 727, 1119
HpyCH4III ACNGT 8 cut(s) 74, 455, 534, 756, 803, 934, 1110, 1158
HpyCH4IV ACGT 2 cut(s) 175, 1022
HpyCH4V TGCA 6 cut(s) 207, 266, 428, 640, 752, 864
HpyF10VI GCNNNNNNNGC 2 cut(s) 651, 794
HpyF3I CTNAG 6 cut(s) 34, 239, 551, 650, 954, 987
HpySE526I ACGT 2 cut(s) 175, 1022
Ksp22I TGATCA 1 cut(s) 126
Kzo9I GATC 6 cut(s) 105, 126, 673, 907, 1162, 1184
LmnI GCTCC 3 cut(s) 62, 82, 689
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 5 cut(s) 194, 437, 627, 976, 1068
MaeII ACGT 2 cut(s) 175, 1022
MaeIII GTNAC 6 cut(s) 458, 934, 1005, 1045, 1112, 1189
MalI GATC 6 cut(s) 107, 128, 675, 909, 1164, 1186
MboI GATC 6 cut(s) 105, 126, 673, 907, 1162, 1184
MboII GAAGA 4 cut(s) 102, 105, 1079, 1159
MflI RGATCY 1 cut(s) 1162
MhlI GDGCHC 3 cut(s) 67, 79, 754
MluCI AATT 9 cut(s) 327, 407, 477, 572, 628, 818, 1130, 1174, 1198
MmeI TCCRAC 1 cut(s) 1140
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 2 cut(s) 12, 344
MslI CAYNNNNRTG 2 cut(s) 47, 113
MspI CCGG 2 cut(s) 912, 1139
MspR9I CCNGG 8 cut(s) 60, 87, 351, 392, 678, 840, 912, 1139
MvaI CCWGG 6 cut(s) 60, 87, 351, 392, 678, 840
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 2 cut(s) 651, 794
NciI CCSGG 2 cut(s) 912, 1139
NdeI CATATG 2 cut(s) 702, 900
NdeII GATC 6 cut(s) 105, 126, 673, 907, 1162, 1184
NmuCI GTSAC 2 cut(s) 1045, 1189
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 4 cut(s) 157, 428, 715, 1046
NspV TTCGAA 2 cut(s) 607, 742
PciI ACATGT 3 cut(s) 153, 711, 1042
PfeI GAWTC 2 cut(s) 706, 1098
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 1 cut(s) 268
PscI ACATGT 3 cut(s) 153, 711, 1042
Psp124BI GAGCTC 1 cut(s) 67
Psp6I CCWGG 6 cut(s) 58, 85, 349, 390, 676, 838
PspGI CCWGG 6 cut(s) 58, 85, 349, 390, 676, 838
PspPI GGNCC 2 cut(s) 394, 836
PsuI RGATCY 1 cut(s) 1162
RsaI GTAC 3 cut(s) 355, 600, 1041
RsaNI GTAC 3 cut(s) 354, 599, 1040
RseI CAYNNNNRTG 2 cut(s) 47, 113
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 2 cut(s) 12, 344
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 6 cut(s) 105, 126, 673, 907, 1162, 1184
Sau96I GGNCC 2 cut(s) 394, 836
ScrFI CCNGG 8 cut(s) 60, 87, 351, 392, 678, 840, 912, 1139
SduI GDGCHC 3 cut(s) 67, 79, 754
SfaNI GCATC 5 cut(s) 194, 437, 627, 976, 1068
SfcI CTRYAG 2 cut(s) 451, 783
SfuI TTCGAA 2 cut(s) 607, 742
SmiMI CAYNNNNRTG 2 cut(s) 47, 113
Sse9I AATT 9 cut(s) 327, 407, 477, 572, 628, 818, 1130, 1174, 1198
SsiI CCGC 3 cut(s) 38, 472, 834
SspI AATATT 2 cut(s) 248, 291
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 8 cut(s) 58, 85, 349, 390, 676, 838, 910, 1137
TaaI ACNGT 8 cut(s) 74, 455, 534, 756, 803, 934, 1110, 1158
TaiI ACGT 2 cut(s) 178, 1025
TaqI TCGA 4 cut(s) 67, 607, 742, 1149
TasI AATT 9 cut(s) 327, 407, 477, 572, 628, 818, 1130, 1174, 1198
TfiI GAWTC 2 cut(s) 706, 1098
Tru1I TTAA 2 cut(s) 12, 344
Tru9I TTAA 2 cut(s) 12, 344
TscAI CASTG 2 cut(s) 129, 761
TseFI GTSAC 2 cut(s) 1045, 1189
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 2 cut(s) 1045, 1189
TspDTI ATGAA 8 cut(s) 17, 183, 325, 414, 719, 782, 887, 888
TspGWI ACGGA 1 cut(s) 1110
TspRI CASTG 2 cut(s) 129, 761
VneI GTGCAC 1 cut(s) 750
XagI CCTNNNNNAGG 1 cut(s) 84
XapI RAATTY 5 cut(s) 407, 477, 818, 1130, 1198
XceI RCATGY 4 cut(s) 157, 428, 715, 1046
XmiI GTMKAC 1 cut(s) 1025
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.