Rorug04G0021500

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004280
Physical Location & Seq
Reverse (-)
3102112 .. 3105633
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0021500.1

Sequence Viewer

Length: 3093 bp
ATGAGTAGGTTTGCCCTGCATGAAAAATTGGGAAGCAATGTTCGGTTGATTCTTGATGTTCCGAGCAGCAGTCTTGGCATAAGCAAAAGAAAATGGCATTCTGCTTTCATTACCATCTACTGCTCTAGAGCCTTCCTCTCTTTACGACCTTCTACTCTTCCCAAACATACCAACTCCACATTCATATCTCGCACACTCTCTTGCTCCATTGTTAGCGTCGAGCCAGTCTCGGTCCCAGCCGTTAATGGATTCCAAGCAGACCAAAAAAGCCTCACTGAGCTTGTGAAGGAAAAAAATCTCAAGGAGCTCTCGGAGCTTGGAGGGGTTGAAGAAGTAGCATCAGCTCTCAAAACTGATGCAGAGCATGGAATCAATGGTGATGATGCTGAAGATATTGCGCGCAGACACGAAGCATTTGGTTCCAATACATACAAGAAACCGCCAACGCAAGGATTCCTCCATTTTGTGTGGGAAGCCTTCAAAGACCTCACAATCCTAATCCTCTTGGGATGTGCTGCGCTTTCTCTTGGTTTCGGCATCAAAGAACATGGATTGAAAGACGGTTGGATTGACGGTGGAAGCATTTGTCTCGCAGTCATTCTGGTCATTTCTGTTTCAGCCATTAGCAACTACAGACAGAGCAGACAGTTTGACAAATTGTCCAAAGTCAGTAACAATCTCCAAATTGAGGCTGTGAGAAATGGAAGGCGCCAGCAGATTTCCATATTTGAAATTGTGGTAGGTGATGTCATTTGTTTGAAGATTGGAGACCAAGTTCCAGCTGATGGATTGCTTTTAGATGGCCATTCTTTATCAGTAGATGAATCAAGCATGACAGGGGAGAGCGACCATGTTGAGGTAAGTGTCACTGAGAATCCTTTCTTGTTCTCTGGGACTAAAATAGCTGATGGTTATGGTCGCATGCTTGTCACGTCAGTCGGGATGAACACAAACTGGGGTGAAATGATGAGCCAAATTAGCCAAGACACCAGTGAAAAGACACCTCTGCAAGCACGTTTAGACAAGCTAACTTCGTCCATCGGTAAAGTTGGTTTGGCAGTTGCTTTCTTTGTTCTTGTAGTCATGTTGGTCCGATACTTCACAGGGAATACAGAGGATGAGAATGGAAACAAAGAGTACAATGGCAGCAAGACAAAATCTAATGACATCATAAATGCCGTTATTGGGATTGTAGCAGCTGCCGTTACAATTGTTGTGGTGGCAATTCCAGAAGGTCTACCATTAGCTGTGACTCTCACTCTTGCTTATTCCATGAAGAGAATGATGGTTGATAACGCAATGGTGCGGAAGCTCTCTGCTTGTGAGACCATGGGATCTGCTACAACAATTTGTACTGACAAAACAGGTACTCTGACCATGAACCAGATGAAGGTGACTAAGGTTTGGTTGGGGAAAGAATGTGTGGAAGAAGAAGCTTATTCATCCATCTCTCCTTATGTTCTCGACTTGATCCAAGAAGGGGTTGCTCTTAATACAACTGGTGGTGTGTACAGGCCTAGCTTGGATTCTGAAATTGAGATCTCAGGCAGTCCAACTGAAAAGGCTATTCTTTCATGGGCGGTACATGGGTCGAACATGGATATGGAGAAAGTCGTGAAGAGTTGTAGCATTCTCCACGTTGAAGCCTTTAATTCGAAGAAGAAACAGAGTGGAGTTCTGGTGAAGAGAAAGGCTGATAACTCAGTCCATGTACACTGGAAAGGGGCAGCAGAGATGATACTAGCAATGTGCACAAGTTACTACAATGCCTCTGGAGTTGTTAAGGATATGGATACAATTGAAAAAAGGAACTTTGAGCAGATTATTCAAGGTATGGCAGCTAGCAGCCTCAGATGCATAGCATTCGCACATAAACAAGTTCCCACTGAAGAGCGACTAAATGCAGACCAGAAAGTTGTGCTAAAGGAAGATGGATTGACCCTATTGGGAGTTGTCGGTCTTAAAGATCCATGTCGTCCCGGTGTGAAGAAAGCAGTTGAAGAATGCCAATATGCAGGGGTGAACGTCAAAATGATTACCGGCGACAATGTTTTCACTGCAAAAGCCATAGCCACTGAATGTGGGATACTCAGGCCTGGTCAAGACATGATTGGAGCAGTGATAGAAGGTGTGGAATTTCGCAACTACACGCCAGAACAGAGAATGGAGAAAGTTGAGAAAATTTGTGTGATGGCAAGGTCTTCTCCTTTTGATAAGCTTCTAATGGTGCAATGCTTGAAACAGAAAGGTCATGTAGTTGCAGTGACGGGTGATGGCACTAATGATGCACCGGCATTAAAAGAAGCTGATATAGGACTTTCCATGGGGATTCAAGGCACAGAAGTTGCCAAGGAAAGCTCAGATATTGTGATCATGGATGATAACTTTGCTTCAGTTGCTACAGTTTTGATGTGGGGAAGATGTGTCTACAATAATATCCAGAAGTTCATCCAATTCCAACTCACCGTCAATGTTGCAGCTCTTGTGATCAACTTTGTGGCAGCAGTTTCGGCAGGTCAAGTCCCATTAACAGCAGTTCAATTATTGTGGGTAAACTTGATCATGGACACACTTGGAGCTCTTGCTCTTGCCACAGAAAAACCCACAAGAGAACTTATGGAGAAGCCACCAGTGGGAAGGACAGCGCCTCTCATCACAAACATCATGTGGAGGAACCTCTTGCCTCAAGCTCTGTACCAGATAACTGTCCTCTTGATCTTACAATTCAGGGGCAAAGCAATCTTCGGTGTCAGTGATAAGGTAAAGGACACATTGATCTTCAACACTTTTGTGCTTTGCCAGATATTTAATGAGTTCAATGCAAGAAAGCTAGAGAAGAAGAATGTCTTCAAGGGAATACACACCAACAAGTTGTTTATGGGGATCATCGCGGTGACAATTGTTCTTCAGGTTGTGATGGTGGAGGTTCTCAAGAAATTTGCAGATACAGAGAGGCTGAATTGGGGACAATGGGGTGTATGCATTGGGATTGCAGTCATCTCTTGGCCAATTGGTTGGCTAGTCAAGTGTATACCTGTTCCAGAGAAACCCATTTTCAGCTATCTGAAGATGAAGAAAAAGCATTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

1030

Amino Acids

112.71

Weight (kDa)

8.08

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 106 - 175 4.3e-15 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 231 - 326 3.5e-21 P-type ATPase actuator domain
Cation_ATPase PF13246 489 - 660 5.8e-28 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 629 - 770 3.5e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 841 - 1010 1.3e-48 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2504
AccB1I GGYRCC 1 cut(s) 708
AccB7I CCANNNNNTGG 1 cut(s) 785
AccI GTMKAC 3 cut(s) 1237, 2427, 3031
AccII CGCG 2 cut(s) 400, 2891
AciI CCGC 4 cut(s) 440, 1306, 1580, 2891
AclWI GGATC 4 cut(s) 1342, 1465, 1961, 2891
AcoI YGGCCR 2 cut(s) 802, 3005
AcsI RAATTY 3 cut(s) 2135, 2181, 2936
AcuI CTGAAG 5 cut(s) 408, 1908, 2376, 2891, 3086
AcyI GRCGYC 1 cut(s) 709
AfaI GTAC 7 cut(s) 1139, 1354, 1369, 1511, 1584, 1713, 2696
AfiI CCNNNNNNNGG 8 cut(s) 449, 688, 785, 856, 1185, 1390, 1480, 2633
AhdI GACNNNNNGTC 1 cut(s) 658
AjiI CACGTC 1 cut(s) 933
AjnI CCWGG 1 cut(s) 2095
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AleI CACNNNNGTG 1 cut(s) 2789
AloI GAACNNNNNNTCC 4 cut(s) 24, 56, 759, 791
Alw21I GWGCWC 3 cut(s) 309, 1754, 2581
Alw26I GTCTC 4 cut(s) 232, 593, 762, 1319
Alw44I GTGCAC 1 cut(s) 1750
AlwI GGATC 4 cut(s) 1342, 1465, 1961, 2891
AoxI GGCC 4 cut(s) 802, 1514, 2093, 3005
ApaLI GTGCAC 1 cut(s) 1750
ApoI RAATTY 3 cut(s) 2135, 2181, 2936
Asp700I GAANNNNTTC 2 cut(s) 878, 2846
AspLEI GCGC 5 cut(s) 400, 402, 520, 711, 2647
AspS9I GGNCC 2 cut(s) 232, 1090
AsuC2I CCSGG 1 cut(s) 1980
AsuHPI GGTGA 9 cut(s) 389, 755, 971, 1405, 1693, 2032, 2282, 2455, 2905
AsuII TTCGAA 1 cut(s) 1655
AsuNHI GCTAGC 1 cut(s) 1841
AvaII GGWCC 2 cut(s) 232, 1090
BaeGI GKGCMC 1 cut(s) 1754
BaeI ACNNNNGTAYC 2 cut(s) 1574, 1607
BalI TGGCCA 2 cut(s) 804, 3007
BanI GGYRCC 1 cut(s) 708
BanII GRGCYC 2 cut(s) 309, 2581
BbsI GAAGAC 2 cut(s) 2193, 2839
Bbv12I GWGCWC 3 cut(s) 309, 1754, 2581
BceAI ACGGC 3 cut(s) 224, 1163, 1187
BcgI CGANNNNNNTGC 6 cut(s) 571, 605, 1846, 1880, 2490, 2524
BciT130I CCWGG 1 cut(s) 2097
BciVI GTATCC 2 cut(s) 1786, 2079
BclI TGATCA 3 cut(s) 2370, 2487, 2559
BcnI CCSGG 1 cut(s) 1980
BcoDI GTCTC 4 cut(s) 232, 593, 762, 1319
BfaI CTAG 6 cut(s) 126, 1518, 1742, 1842, 2831, 3020
BfmI CTRYAG 2 cut(s) 631, 2400
BfoI RGCGCY 2 cut(s) 712, 2648
BfuAI ACCTGC 1 cut(s) 2504
BfuI GTATCC 2 cut(s) 1786, 2079
BglII AGATCT 1 cut(s) 1539
Bme1390I CCNGG 2 cut(s) 1980, 2097
Bme18I GGWCC 2 cut(s) 232, 1090
BmeRI GACNNNNNGTC 1 cut(s) 658
BmgBI CACGTC 1 cut(s) 933
BmgT120I GGNCC 2 cut(s) 232, 1090
BmiI GGNNCC 4 cut(s) 234, 421, 710, 2675
BmrFI CCNGG 2 cut(s) 1980, 2097
BmrI ACTGGG 1 cut(s) 964
BmsI GCATC 6 cut(s) 346, 347, 373, 546, 1844, 2275
BmtI GCTAGC 1 cut(s) 1845
BmuI ACTGGG 1 cut(s) 964
BpiI GAAGAC 2 cut(s) 2193, 2839
BplI GAGNNNNNCTC 4 cut(s) 120, 152, 212, 244
BpmI CTGGAG 1 cut(s) 1794
Bpu14I TTCGAA 1 cut(s) 1655
BpuEI CTTGAG 3 cut(s) 284, 2670, 2915
BpuMI CCSGG 1 cut(s) 1980
BsaHI GRCGYC 1 cut(s) 709
BsaI GGTCTC 2 cut(s) 762, 1319
BsaJI CCNNGG 3 cut(s) 1329, 2322, 2349
BsaXI ACNNNNNCTCC 4 cut(s) 759, 789, 1665, 1695
Bsc4I CCNNNNNNNGG 8 cut(s) 449, 688, 785, 856, 1185, 1390, 1480, 2633
Bse118I RCCGGY 2 cut(s) 2039, 2290
Bse1I ACTGG 6 cut(s) 224, 959, 990, 1504, 1721, 2630
Bse3DI GCAATG 4 cut(s) 43, 1305, 1752, 2237
BseBI CCWGG 1 cut(s) 2097
BseDI CCNNGG 3 cut(s) 1329, 2322, 2349
BseGI GGATG 6 cut(s) 515, 948, 1123, 1442, 2383, 2448
BseLI CCNNNNNNNGG 8 cut(s) 449, 688, 785, 856, 1185, 1390, 1480, 2633
BseMI GCAATG 4 cut(s) 43, 1305, 1752, 2237
BseMII CTCAG 7 cut(s) 267, 861, 1557, 1716, 1864, 2104, 2373
BseNI ACTGG 6 cut(s) 224, 959, 990, 1504, 1721, 2630
BsePI GCGCGC 1 cut(s) 398
BseSI GKGCMC 1 cut(s) 1754
BseYI CCCAGC 1 cut(s) 235
Bsh1236I CGCG 2 cut(s) 400, 2891
BshFI GGCC 4 cut(s) 804, 1516, 2095, 3007
BshNI GGYRCC 1 cut(s) 708
BsiHKAI GWGCWC 3 cut(s) 309, 1754, 2581
BsiSI CCGG 3 cut(s) 1980, 2040, 2291
BslFI GGGAC 5 cut(s) 218, 907, 1962, 2507, 2979
BslI CCNNNNNNNGG 8 cut(s) 449, 688, 785, 856, 1185, 1390, 1480, 2633
BsmAI GTCTC 4 cut(s) 232, 593, 762, 1319
BsmFI GGGAC 5 cut(s) 218, 907, 1962, 2507, 2979
BsmI GAATGC 5 cut(s) 97, 1629, 1862, 2009, 3082
BsnI GGCC 4 cut(s) 804, 1516, 2095, 3007
Bso31I GGTCTC 2 cut(s) 762, 1319
Bsp119I TTCGAA 1 cut(s) 1655
Bsp1286I GDGCHC 3 cut(s) 309, 1754, 2581
Bsp1407I TGTACA 2 cut(s) 1509, 1711
Bsp19I CCATGG 2 cut(s) 1329, 2322
BspACI CCGC 4 cut(s) 440, 1306, 1580, 2891
BspANI GGCC 4 cut(s) 804, 1516, 2095, 3007
BspCNI CTCAG 7 cut(s) 268, 862, 1556, 1715, 1863, 2103, 2372
BspFNI CGCG 2 cut(s) 400, 2891
BspLI GGNNCC 4 cut(s) 234, 421, 710, 2675
BspMI ACCTGC 1 cut(s) 2504
BspOI GCTAGC 1 cut(s) 1845
BspPI GGATC 4 cut(s) 1342, 1465, 1961, 2891
BspQI GCTCTTC 1 cut(s) 1884
BspT104I TTCGAA 1 cut(s) 1655
BspT107I GGYRCC 1 cut(s) 708
BspTNI GGTCTC 2 cut(s) 762, 1319
BsrDI GCAATG 4 cut(s) 43, 1305, 1752, 2237
BsrFI RCCGGY 2 cut(s) 2039, 2290
BsrGI TGTACA 2 cut(s) 1509, 1711
BsrI ACTGG 6 cut(s) 224, 959, 990, 1504, 1721, 2630
BssAI RCCGGY 2 cut(s) 2039, 2290
BssECI CCNNGG 3 cut(s) 1329, 2322, 2349
BssHII GCGCGC 1 cut(s) 398
BssNAI GTATAC 1 cut(s) 3032
BssNI GRCGYC 1 cut(s) 709
BssT1I CCWWGG 3 cut(s) 1329, 2322, 2349
Bst1107I GTATAC 1 cut(s) 3032
Bst2UI CCWGG 1 cut(s) 2097
Bst4CI ACNGT 6 cut(s) 563, 575, 648, 2404, 2467, 2707
Bst6I CTCTTC 5 cut(s) 162, 1271, 1613, 1679, 1884
BstACI GRCGYC 1 cut(s) 709
BstAUI TGTACA 2 cut(s) 1509, 1711
BstBI TTCGAA 1 cut(s) 1655
BstC8I GCNNGC 5 cut(s) 400, 713, 923, 1011, 1843
BstDEI CTNAG 8 cut(s) 276, 870, 1398, 1543, 1702, 1850, 2090, 2359
BstDSI CCRYGG 2 cut(s) 1329, 2322
BstF5I GGATG 6 cut(s) 515, 948, 1123, 1442, 2383, 2448
BstFNI CGCG 2 cut(s) 400, 2891
BstH2I RGCGCY 2 cut(s) 712, 2648
BstHHI GCGC 5 cut(s) 400, 402, 520, 711, 2647
BstMAI GTCTC 4 cut(s) 232, 593, 762, 1319
BstMWI GCNNNNNNNGC 6 cut(s) 75, 313, 978, 1854, 2396, 2510
BstNI CCWGG 1 cut(s) 2097
BstNSI RCATGY 1 cut(s) 925
BstSCI CCNGG 2 cut(s) 1978, 2095
BstSFI CTRYAG 2 cut(s) 631, 2400
BstSLI GKGCMC 1 cut(s) 1754
BstUI CGCG 2 cut(s) 400, 2891
BstV2I GAAGAC 2 cut(s) 2193, 2839
BstX2I RGATCY 3 cut(s) 1334, 1539, 1966
BstXI CCANNNNNNTGG 1 cut(s) 3015
BstYI RGATCY 3 cut(s) 1334, 1539, 1966
BstZ17I GTATAC 1 cut(s) 3032
BsuI GTATCC 2 cut(s) 1786, 2079
BsuRI GGCC 4 cut(s) 804, 1516, 2095, 3007
BtgI CCRYGG 2 cut(s) 1329, 2322
BtgZI GCGATG 1 cut(s) 2872
BtrI CACGTC 1 cut(s) 933
BtsCI GGATG 6 cut(s) 515, 948, 1123, 1442, 2383, 2448
BtsI GCAGTG 3 cut(s) 2055, 2124, 2268
BveI ACCTGC 1 cut(s) 2504
Cac8I GCNNGC 5 cut(s) 400, 713, 923, 1011, 1843
CfoI GCGC 5 cut(s) 400, 402, 520, 711, 2647
Cfr10I RCCGGY 2 cut(s) 2039, 2290
Cfr13I GGNCC 2 cut(s) 232, 1090
CseI GACGC 1 cut(s) 205
Csp6I GTAC 7 cut(s) 1138, 1353, 1368, 1510, 1583, 1712, 2695
CspCI CAANNNNNGTGG 4 cut(s) 1197, 1232, 2528, 2563
CviQI GTAC 7 cut(s) 1138, 1353, 1368, 1510, 1583, 1712, 2695
DdeI CTNAG 8 cut(s) 276, 870, 1398, 1543, 1702, 1850, 2090, 2359
DinI GGCGCC 1 cut(s) 710
DriI GACNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 2 cut(s) 802, 3005
Eam1104I CTCTTC 5 cut(s) 162, 1271, 1613, 1679, 1884
Eam1105I GACNNNNNGTC 1 cut(s) 658
EarI CTCTTC 5 cut(s) 162, 1271, 1613, 1679, 1884
Ecl136II GAGCTC 2 cut(s) 307, 2579
Eco130I CCWWGG 3 cut(s) 1329, 2322, 2349
Eco147I AGGCCT 2 cut(s) 1516, 2095
Eco24I GRGCYC 2 cut(s) 309, 2581
Eco31I GGTCTC 2 cut(s) 762, 1319
Eco47I GGWCC 2 cut(s) 232, 1090
Eco53kI GAGCTC 2 cut(s) 307, 2579
Eco57I CTGAAG 5 cut(s) 408, 1908, 2376, 2891, 3086
EcoICRI GAGCTC 2 cut(s) 307, 2579
EcoRII CCWGG 1 cut(s) 2095
EcoT14I CCWWGG 3 cut(s) 1329, 2322, 2349
EcoT22I ATGCAT 2 cut(s) 1859, 2984
EcoT38I GRGCYC 2 cut(s) 309, 2581
EgeI GGCGCC 1 cut(s) 710
EheI GGCGCC 1 cut(s) 710
ErhI CCWWGG 3 cut(s) 1329, 2322, 2349
FalI AAGNNNNNCTT 2 cut(s) 2831, 2863
FaqI GGGAC 5 cut(s) 218, 907, 1962, 2507, 2979
FbaI TGATCA 3 cut(s) 2370, 2487, 2559
FblI GTMKAC 3 cut(s) 1237, 2427, 3031
FokI GGATG 6 cut(s) 522, 955, 1130, 1429, 2390, 2435
FriOI GRGCYC 2 cut(s) 309, 2581
FspBI CTAG 6 cut(s) 126, 1518, 1742, 1842, 2831, 3020
GlaI GCGC 5 cut(s) 399, 401, 519, 710, 2646
GsaI CCCAGC 1 cut(s) 239
GsuI CTGGAG 1 cut(s) 1794
HaeII RGCGCY 2 cut(s) 712, 2648
HaeIII GGCC 4 cut(s) 804, 1516, 2095, 3007
HapII CCGG 3 cut(s) 1980, 2040, 2291
HgaI GACGC 1 cut(s) 205
HhaI GCGC 5 cut(s) 400, 402, 520, 711, 2647
Hin1I GRCGYC 1 cut(s) 709
Hin6I GCGC 5 cut(s) 398, 400, 518, 709, 2645
HinP1I GCGC 5 cut(s) 398, 400, 518, 709, 2645
HindIII AAGCTT 2 cut(s) 1434, 2216
HinfI GANTC 9 cut(s) 49, 249, 369, 453, 824, 874, 1252, 1526, 2329
HpaII CCGG 3 cut(s) 1980, 2040, 2291
HphI GGTGA 9 cut(s) 389, 755, 971, 1405, 1693, 2032, 2282, 2455, 2905
Hpy166II GTNNAC 8 cut(s) 1238, 1510, 1714, 1752, 2023, 2428, 2554, 3032
Hpy188I TCNGA 8 cut(s) 63, 313, 1094, 1374, 1531, 1853, 2362, 3066
Hpy8I GTNNAC 8 cut(s) 1238, 1510, 1714, 1752, 2023, 2428, 2554, 3032
Hpy99I CGWCG 1 cut(s) 221
HpyCH4III ACNGT 6 cut(s) 563, 575, 648, 2404, 2467, 2707
HpyCH4IV ACGT 4 cut(s) 932, 1015, 1638, 2025
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 313, 978, 1854, 2396, 2510
HpyF3I CTNAG 8 cut(s) 276, 870, 1398, 1543, 1702, 1850, 2090, 2359
HpySE526I ACGT 4 cut(s) 932, 1015, 1638, 2025
Hsp92I GRCGYC 1 cut(s) 709
HspAI GCGC 5 cut(s) 398, 400, 518, 709, 2645
KasI GGCGCC 1 cut(s) 708
Ksp22I TGATCA 3 cut(s) 2370, 2487, 2559
LguI GCTCTTC 1 cut(s) 1884
LmnI GCTCC 5 cut(s) 209, 304, 313, 2114, 2576
LweI GCATC 6 cut(s) 346, 347, 373, 546, 1844, 2275
MaeI CTAG 6 cut(s) 126, 1518, 1742, 1842, 2831, 3020
MaeII ACGT 4 cut(s) 932, 1015, 1638, 2025
MaeIII GTNAC 9 cut(s) 671, 865, 928, 1204, 1249, 1393, 1757, 2263, 2893
MfeI CAATTG 4 cut(s) 1209, 1797, 2898, 3009
MflI RGATCY 3 cut(s) 1334, 1539, 1966
MhlI GDGCHC 3 cut(s) 309, 1754, 2581
MlsI TGGCCA 2 cut(s) 804, 3007
MluNI TGGCCA 2 cut(s) 804, 3007
Mly113I GGCGCC 1 cut(s) 709
MlyI GAGTC 1 cut(s) 1246
MmeI TCCRAC 3 cut(s) 545, 1577, 2482
Mox20I TGGCCA 2 cut(s) 804, 3007
Mph1103I ATGCAT 2 cut(s) 1859, 2984
MroXI GAANNNNTTC 2 cut(s) 878, 2846
MscI TGGCCA 2 cut(s) 804, 3007
MseI TTAA 8 cut(s) 243, 1491, 1650, 1782, 1962, 2297, 2528, 2808
MslI CAYNNNNRTG 3 cut(s) 1601, 2789, 2891
Msp20I TGGCCA 2 cut(s) 804, 3007
MspA1I CMGCKG 2 cut(s) 782, 1199
MspI CCGG 3 cut(s) 1980, 2040, 2291
MspR9I CCNGG 2 cut(s) 1980, 2097
MunI CAATTG 4 cut(s) 1209, 1797, 2898, 3009
Mva1269I GAATGC 5 cut(s) 97, 1629, 1862, 2009, 3082
MvaI CCWGG 1 cut(s) 2097
MvnI CGCG 2 cut(s) 400, 2891
MwoI GCNNNNNNNGC 6 cut(s) 75, 313, 978, 1854, 2396, 2510
NarI GGCGCC 1 cut(s) 709
NciI CCSGG 1 cut(s) 1980
NcoI CCATGG 2 cut(s) 1329, 2322
NheI GCTAGC 1 cut(s) 1841
NlaIV GGNNCC 4 cut(s) 234, 421, 710, 2675
NmuCI GTSAC 6 cut(s) 865, 928, 1249, 1393, 2263, 2893
NsiI ATGCAT 2 cut(s) 1859, 2984
NspI RCATGY 1 cut(s) 925
NspV TTCGAA 1 cut(s) 1655
OliI CACNNNNGTG 1 cut(s) 2789
PaeI GCATGC 1 cut(s) 925
PauI GCGCGC 1 cut(s) 398
PceI AGGCCT 2 cut(s) 1516, 2095
PciSI GCTCTTC 1 cut(s) 1884
PctI GAATGC 5 cut(s) 97, 1629, 1862, 2009, 3082
PdmI GAANNNNTTC 2 cut(s) 878, 2846
PfeI GAWTC 8 cut(s) 49, 249, 369, 453, 824, 874, 1526, 2329
PflMI CCANNNNNTGG 1 cut(s) 785
PleI GAGTC 1 cut(s) 1246
PluTI GGCGCC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 1246
Psp124BI GAGCTC 2 cut(s) 309, 2581
Psp6I CCWGG 1 cut(s) 2095
PspFI CCCAGC 1 cut(s) 235
PspGI CCWGG 1 cut(s) 2095
PspN4I GGNNCC 4 cut(s) 234, 421, 710, 2675
PspPI GGNCC 2 cut(s) 232, 1090
PsuI RGATCY 3 cut(s) 1334, 1539, 1966
PteI GCGCGC 1 cut(s) 398
PvuII CAGCTG 2 cut(s) 782, 1199
RsaI GTAC 7 cut(s) 1139, 1354, 1369, 1511, 1584, 1713, 2696
RsaNI GTAC 7 cut(s) 1138, 1353, 1368, 1510, 1583, 1712, 2695
RseI CAYNNNNRTG 3 cut(s) 1601, 2789, 2891
SacI GAGCTC 2 cut(s) 309, 2581
SapI GCTCTTC 1 cut(s) 1884
SaqAI TTAA 8 cut(s) 243, 1491, 1650, 1782, 1962, 2297, 2528, 2808
Sau96I GGNCC 2 cut(s) 232, 1090
SchI GAGTC 1 cut(s) 1246
ScrFI CCNGG 2 cut(s) 1980, 2097
SduI GDGCHC 3 cut(s) 309, 1754, 2581
SfaNI GCATC 6 cut(s) 346, 347, 373, 546, 1844, 2275
SfcI CTRYAG 2 cut(s) 631, 2400
SfoI GGCGCC 1 cut(s) 710
SfuI TTCGAA 1 cut(s) 1655
SinI GGWCC 2 cut(s) 232, 1090
SmiMI CAYNNNNRTG 3 cut(s) 1601, 2789, 2891
SmlI CTYRAG 3 cut(s) 299, 2685, 2930
SmoI CTYRAG 3 cut(s) 299, 2685, 2930
SphI GCATGC 1 cut(s) 925
SseBI AGGCCT 2 cut(s) 1516, 2095
SsiI CCGC 4 cut(s) 440, 1306, 1580, 2891
SspDI GGCGCC 1 cut(s) 708
SspMI CTAG 6 cut(s) 126, 1518, 1742, 1842, 2831, 3020
SstI GAGCTC 2 cut(s) 309, 2581
StuI AGGCCT 2 cut(s) 1516, 2095
StyD4I CCNGG 2 cut(s) 1978, 2095
StyI CCWWGG 3 cut(s) 1329, 2322, 2349
TaaI ACNGT 6 cut(s) 563, 575, 648, 2404, 2467, 2707
TaiI ACGT 4 cut(s) 935, 1018, 1641, 2028
TaqI TCGA 4 cut(s) 219, 1464, 1592, 1655
TaqII GACCGA 2 cut(s) 220, 1946
TatI WGTACW 4 cut(s) 1137, 1352, 1509, 1711
TfiI GAWTC 8 cut(s) 49, 249, 369, 453, 824, 874, 1526, 2329
Tru1I TTAA 8 cut(s) 243, 1491, 1650, 1782, 1962, 2297, 2528, 2808
Tru9I TTAA 8 cut(s) 243, 1491, 1650, 1782, 1962, 2297, 2528, 2808
TseFI GTSAC 6 cut(s) 865, 928, 1249, 1393, 2263, 2893
Tsp45I GTSAC 6 cut(s) 865, 928, 1249, 1393, 2263, 2893
Van91I CCANNNNNTGG 1 cut(s) 785
VneI GTGCAC 1 cut(s) 1750
VpaK11BI GGWCC 2 cut(s) 232, 1090
XapI RAATTY 3 cut(s) 2135, 2181, 2936
XbaI TCTAGA 1 cut(s) 125
XceI RCATGY 1 cut(s) 925
XmiI GTMKAC 3 cut(s) 1237, 2427, 3031
XmnI GAANNNNTTC 2 cut(s) 878, 2846
XspI CTAG 6 cut(s) 126, 1518, 1742, 1842, 2831, 3020
Zsp2I ATGCAT 2 cut(s) 1859, 2984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.