Rh4CG106600

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004280
Physical Location & Seq
Reverse (-)
19683707 .. 19689953
6247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG106600.1

Sequence Viewer

Length: 1179 bp
ATGAAGACGGGTCAGCCGTGGCGAATAGGCATGGGTGACATTCCTATATTTTCTGGGGCTCGCCATCGCTCACCTTTGAAGAGGCCATTGTGGATCATTGTCTTGGTGTCTTTGGTTAGCATCTTTGTGATCTGTGCTTTTCTCTATCAACCGCAAAGCAACGCCGCTTGTTACATATTTTCTTCAAGAGGTTGTAAGGCTATTTCCGATTGGCTTCCACCTGCTCCAGCAAGGGAATATAATGATGATGAGATTGCAGCCCGAGTAGTGATAAAAGAAATTTTGAGTGCACCTCCTGTTCAATCAAAAAACCCGAAAATAGCATTCATGTTCCTTACACCAAGTGCAGTGCCTTTTGAGAAGCTGTGGGATAAATTTTTTCATGGTCATGAGGGAAAGTTCTCTGTTTATGTGCATGCATCTAAAGAAAAGGCAGTTCATTTGAGCCGTTACTTTGCTAATAGAGAGATACGCAGTGATCAGGTGATTTGGGGAAAAATTTCCATGGTAGATGCTGAGAGGCGATTATTGGCAAATGCTCTTCAAGATCCTGATAACCAGCACTTTGTGCTACTTTCTGAAAGTTGTGTACCGTTGTATACGTTTGACTATATCTATGACTATCTGATGAATACAAATATAAGCTATGTTGACTGCTTTGAGGATCCTGGCCCACATGGAAATGGCAGGTACTCAGAACACATGTTACCTGAAATTGAGAAGAAAGACTTTAGAAAGGGTGCACAGTGGTTCTCTGTTAAGCGTCAGCATGCTGTTATAATAATGGCTGATAGTCTTTACTACTCAAAATTCCGGGATTACTGCAAGCCAGGTTTGGAGGGGCGCAATTGCATTGCTGATGAACATTACTTGCCAACCTTTTTCAATATAGTCGATCCTGGTGGAATTGCAAACTGGTCTGTAACACATGTTGATTGGTCTGAAAGAAAGTGGCACCCAAAGTTATACAAGGCTCAGGATATTAGTTATGAACTCCTGAGAAATATTACGTCGATTGATGTGAGTGTCCATGTAACCAGTGATGAAAAGAGGGTAGTGCAAAGATGGCCTTGCTTATGGAATGGTATACAACGACCGTGTTACTTGTTCGCAAGGAAATTCCATCCAGAGAGTCTCAATAACTTGTTGCACCTTTTTGCCAATTATACAACAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

392

Amino Acids

45.43

Weight (kDa)

8.59

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 107 - 336 1.3e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 779
AarI CACCTGC 1 cut(s) 229
Acc36I ACCTGC 2 cut(s) 229, 678
AccB1I GGYRCC 1 cut(s) 954
AccI GTMKAC 2 cut(s) 599, 1087
AciI CCGC 2 cut(s) 152, 165
AclWI GGATC 5 cut(s) 101, 542, 659, 672, 890
AcsI RAATTY 5 cut(s) 279, 374, 498, 809, 1118
AdeI CACNNNGTG 2 cut(s) 344, 568
AfaI GTAC 2 cut(s) 591, 692
AflIII ACRYGT 2 cut(s) 702, 928
AgsI TTSAA 5 cut(s) 79, 186, 302, 545, 886
AjnI CCWGG 3 cut(s) 667, 829, 898
AluBI AGCT 2 cut(s) 364, 645
AluI AGCT 2 cut(s) 364, 645
Alw21I GWGCWC 2 cut(s) 292, 745
Alw26I GTCTC 1 cut(s) 1139
Alw44I GTGCAC 2 cut(s) 288, 741
AlwI GGATC 5 cut(s) 101, 542, 659, 672, 890
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 3 cut(s) 83, 670, 1067
ApaLI GTGCAC 2 cut(s) 288, 741
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 5 cut(s) 279, 374, 498, 809, 1118
Asp700I GAANNNNTTC 1 cut(s) 499
AspLEI GCGC 1 cut(s) 846
AspS9I GGNCC 1 cut(s) 671
AsuC2I CCSGG 1 cut(s) 815
AsuHPI GGTGA 3 cut(s) 47, 63, 496
AvaI CYCGRG 1 cut(s) 261
BaeGI GKGCMC 2 cut(s) 292, 745
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 292, 745
BbvI GCAGC 1 cut(s) 269
BccI CCATC 3 cut(s) 72, 1059, 1131
BceAI ACGGC 1 cut(s) 432
BciT130I CCWGG 3 cut(s) 669, 831, 900
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 1 cut(s) 815
BcoDI GTCTC 1 cut(s) 1139
BfuAI ACCTGC 2 cut(s) 229, 678
BisI GCNGC 2 cut(s) 165, 258
BlsI GCNGC 2 cut(s) 166, 259
Bme1390I CCNGG 4 cut(s) 669, 815, 831, 900
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 2 cut(s) 666, 956
BmrFI CCNGG 4 cut(s) 669, 815, 831, 900
BmsI GCATC 3 cut(s) 129, 428, 502
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 975
BpuMI CCSGG 1 cut(s) 815
BsaJI CCNNGG 2 cut(s) 17, 504
Bse1I ACTGG 2 cut(s) 920, 1038
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 3 cut(s) 669, 831, 900
BseDI CCNNGG 2 cut(s) 17, 504
BseGI GGATG 1 cut(s) 1123
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 4 cut(s) 507, 708, 989, 989
BseNI ACTGG 2 cut(s) 920, 1038
BseSI GKGCMC 2 cut(s) 292, 745
BseXI GCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 366
Bsh1285I CGRYCG 1 cut(s) 1097
BshFI GGCC 3 cut(s) 85, 672, 1069
BshNI GGYRCC 1 cut(s) 954
BsiEI CGRYCG 1 cut(s) 1097
BsiHKAI GWGCWC 2 cut(s) 292, 745
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 1 cut(s) 814
BsmAI GTCTC 1 cut(s) 1139
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 3 cut(s) 85, 672, 1069
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 61, 292, 745
Bsp143I GATC 6 cut(s) 93, 129, 478, 547, 664, 895
Bsp19I CCATGG 1 cut(s) 504
BspACI CCGC 2 cut(s) 152, 165
BspANI GGCC 3 cut(s) 85, 672, 1069
BspCNI CTCAG 4 cut(s) 508, 707, 988, 990
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 2 cut(s) 666, 956
BspMI ACCTGC 2 cut(s) 229, 678
BspPI GGATC 5 cut(s) 101, 542, 659, 672, 890
BspQI GCTCTTC 1 cut(s) 546
BspT107I GGYRCC 1 cut(s) 954
BsrDI GCAATG 1 cut(s) 852
BsrI ACTGG 2 cut(s) 920, 1038
BssECI CCNNGG 2 cut(s) 17, 504
BssMI GATC 6 cut(s) 93, 129, 478, 547, 664, 895
BssNAI GTATAC 2 cut(s) 600, 1088
BssT1I CCWWGG 1 cut(s) 504
Bst1107I GTATAC 2 cut(s) 600, 1088
Bst2UI CCWGG 3 cut(s) 669, 831, 900
Bst4CI ACNGT 3 cut(s) 594, 747, 1098
Bst6I CTCTTC 2 cut(s) 74, 546
BstAPI GCANNNNNTGC 1 cut(s) 568
BstC8I GCNNGC 4 cut(s) 61, 417, 771, 827
BstDEI CTNAG 4 cut(s) 516, 694, 975, 998
BstDSI CCRYGG 2 cut(s) 17, 504
BstF5I GGATG 1 cut(s) 1123
BstHHI GCGC 1 cut(s) 846
BstKTI GATC 6 cut(s) 96, 132, 481, 550, 667, 898
BstMAI GTCTC 1 cut(s) 1139
BstMBI GATC 6 cut(s) 93, 129, 478, 547, 664, 895
BstMCI CGRYCG 1 cut(s) 1097
BstMWI GCNNNNNNNGC 2 cut(s) 568, 1066
BstNI CCWGG 3 cut(s) 669, 831, 900
BstNSI RCATGY 4 cut(s) 419, 706, 773, 932
BstSCI CCNGG 4 cut(s) 667, 813, 829, 898
BstSLI GKGCMC 2 cut(s) 292, 745
BstV1I GCAGC 1 cut(s) 269
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 547, 664
BstYI RGATCY 2 cut(s) 547, 664
BstZ17I GTATAC 2 cut(s) 600, 1088
BsuRI GGCC 3 cut(s) 85, 672, 1069
BtgI CCRYGG 2 cut(s) 17, 504
BtgZI GCGATG 1 cut(s) 50
BtsCI GGATG 1 cut(s) 1123
BtsI GCAGTG 2 cut(s) 354, 481
BtsIMutI CAGTG 4 cut(s) 354, 481, 752, 1045
BveI ACCTGC 2 cut(s) 229, 678
Cac8I GCNNGC 4 cut(s) 61, 417, 771, 827
CciI TCATGA 1 cut(s) 388
CfoI GCGC 1 cut(s) 846
Cfr13I GGNCC 1 cut(s) 671
CseI GACGC 1 cut(s) 752
Csp6I GTAC 2 cut(s) 590, 691
CviQI GTAC 2 cut(s) 590, 691
DdeI CTNAG 4 cut(s) 516, 694, 975, 998
DpnI GATC 6 cut(s) 95, 131, 480, 549, 666, 897
DpnII GATC 6 cut(s) 93, 129, 478, 547, 664, 895
DraIII CACNNNGTG 2 cut(s) 344, 568
Eam1104I CTCTTC 2 cut(s) 74, 546
EarI CTCTTC 2 cut(s) 74, 546
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 261
EcoRII CCWGG 3 cut(s) 667, 829, 898
EcoT14I CCWWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 504
FalI AAGNNNNNCTT 4 cut(s) 713, 745, 1054, 1086
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 2 cut(s) 599, 1087
Fnu4HI GCNGC 2 cut(s) 165, 258
FokI GGATG 1 cut(s) 1110
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 2 cut(s) 165, 258
GlaI GCGC 1 cut(s) 845
GluI GCNGC 2 cut(s) 165, 258
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 3 cut(s) 85, 672, 1069
HapII CCGG 1 cut(s) 814
HgaI GACGC 1 cut(s) 752
HhaI GCGC 1 cut(s) 846
Hin6I GCGC 1 cut(s) 844
HinP1I GCGC 1 cut(s) 844
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 1 cut(s) 1132
HpaII CCGG 1 cut(s) 814
HphI GGTGA 3 cut(s) 47, 63, 496
Hpy166II GTNNAC 6 cut(s) 290, 590, 600, 652, 743, 1088
Hpy188I TCNGA 5 cut(s) 208, 580, 627, 697, 943
Hpy188III TCNNGA 7 cut(s) 186, 389, 545, 551, 977, 997, 1127
Hpy8I GTNNAC 6 cut(s) 290, 590, 600, 652, 743, 1088
Hpy99I CGWCG 1 cut(s) 1015
HpyCH4III ACNGT 3 cut(s) 594, 747, 1098
HpyCH4IV ACGT 2 cut(s) 602, 1010
HpyF10VI GCNNNNNNNGC 2 cut(s) 568, 1066
HpyF3I CTNAG 4 cut(s) 516, 694, 975, 998
HpySE526I ACGT 2 cut(s) 602, 1010
HspAI GCGC 1 cut(s) 844
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 93, 129, 478, 547, 664, 895
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 1 cut(s) 269
LweI GCATC 3 cut(s) 129, 428, 502
MaeII ACGT 2 cut(s) 602, 1010
MaeIII GTNAC 7 cut(s) 35, 170, 449, 705, 922, 1033, 1100
MalI GATC 6 cut(s) 95, 131, 480, 549, 666, 897
MboI GATC 6 cut(s) 93, 129, 478, 547, 664, 895
MboII GAAGA 5 cut(s) 16, 91, 174, 533, 733
MfeI CAATTG 1 cut(s) 847
MflI RGATCY 2 cut(s) 547, 664
MhlI GDGCHC 3 cut(s) 61, 292, 745
MlyI GAGTC 1 cut(s) 1141
MnlI CCTC 8 cut(s) 75, 182, 303, 385, 513, 655, 832, 1044
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 1 cut(s) 759
MslI CAYNNNNRTG 3 cut(s) 125, 387, 681
MspI CCGG 1 cut(s) 814
MspR9I CCNGG 4 cut(s) 669, 815, 831, 900
MunI CAATTG 1 cut(s) 847
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 3 cut(s) 669, 831, 900
MwoI GCNNNNNNNGC 2 cut(s) 568, 1066
NciI CCSGG 1 cut(s) 815
NcoI CCATGG 1 cut(s) 504
NdeII GATC 6 cut(s) 93, 129, 478, 547, 664, 895
NlaIV GGNNCC 2 cut(s) 666, 956
NmuCI GTSAC 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 4 cut(s) 419, 706, 773, 932
PaeI GCATGC 2 cut(s) 419, 773
PagI TCATGA 1 cut(s) 388
PaqCI CACCTGC 1 cut(s) 229
PciI ACATGT 2 cut(s) 702, 928
PciSI GCTCTTC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PctI GAATGC 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 499
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 2 cut(s) 166, 259
PleI GAGTC 1 cut(s) 1140
PpsI GAGTC 1 cut(s) 1140
PscI ACATGT 2 cut(s) 702, 928
PsiI TTATAA 1 cut(s) 779
Psp6I CCWGG 3 cut(s) 667, 829, 898
PspGI CCWGG 3 cut(s) 667, 829, 898
PspN4I GGNNCC 2 cut(s) 666, 956
PspPI GGNCC 1 cut(s) 671
PsrI GAACNNNNNNTAC 2 cut(s) 690, 722
PsuI RGATCY 2 cut(s) 547, 664
RsaI GTAC 2 cut(s) 591, 692
RsaNI GTAC 2 cut(s) 590, 691
RseI CAYNNNNRTG 3 cut(s) 125, 387, 681
SapI GCTCTTC 1 cut(s) 546
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 2 cut(s) 165, 258
Sau3AI GATC 6 cut(s) 93, 129, 478, 547, 664, 895
Sau96I GGNCC 1 cut(s) 671
SchI GAGTC 1 cut(s) 1141
ScrFI CCNGG 4 cut(s) 669, 815, 831, 900
SduI GDGCHC 3 cut(s) 61, 292, 745
SfaNI GCATC 3 cut(s) 129, 428, 502
SmiMI CAYNNNNRTG 3 cut(s) 125, 387, 681
SphI GCATGC 2 cut(s) 419, 773
SsiI CCGC 2 cut(s) 152, 165
SspI AATATT 1 cut(s) 1006
StyD4I CCNGG 4 cut(s) 667, 813, 829, 898
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 3 cut(s) 594, 747, 1098
TaiI ACGT 2 cut(s) 605, 1013
TaqI TCGA 2 cut(s) 894, 1013
TauI GCSGC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 4 cut(s) 354, 481, 752, 1045
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 8 cut(s) 17, 316, 371, 428, 644, 876, 1005, 1059
TspRI CASTG 4 cut(s) 354, 481, 752, 1045
VneI GTGCAC 2 cut(s) 288, 741
XapI RAATTY 5 cut(s) 279, 374, 498, 809, 1118
XceI RCATGY 4 cut(s) 419, 706, 773, 932
XmiI GTMKAC 2 cut(s) 599, 1087
XmnI GAANNNNTTC 1 cut(s) 499
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.