Rorug04G0021600

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004280
Physical Location & Seq
Reverse (-)
3112306 .. 3113190
885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0021600.1

Sequence Viewer

Length: 885 bp
ATGGAGAAGGTTGAGAAAATTTGTGTGATGGCAAGGTCTTCTCCTTTTGATAAGCTTCTAATGGTGCAATGCTTGAAACAGAAAGGTCATGTAGTTGCAGTGACGGGTGATGGCACTAATGATGCACCAGCATTAAAAGAAGCTGATATAGGACTTTCCATGGGGATTCAAGGCACAGAAGTTGCCAAGGAAAGCTCAGATATTGTGATCATGGATGATAACTTTGCTTCAGTTGCTACAGTTTTGATGTGGGGAAGATGTGTCTACAATAATATCCAGAAGTTCATCCAATTGCAACTCACCGTCAATGTTGCAGCTCTTGTGATCAACTTTGTGGCAGCAGTTTCAGCAGGTCAAGTCCCATTAACAGCAGTTCAATTATTGTGGGTAAACTTGATCATGGACACACTTGGAGCTCTTGCTCTTGCCACAGAAAAACCCACAAGAGAACTTATGGAGAAGCCACCAGTGGGAAGGACGGCACCTCTAATCACAAACATCATGTGGAGGAATCTCTTGCCTCAAGCACTGTACCAAATAACAGTCCTCTTGATCTTACAATTCCGGGGAAAAGCAATCTTTGGTGTCAGTGATAAGGTAAAGGACACATTGATCTTCAACACTTTTGTGCTTTGCCAGGTTTTCAATGTGTTCAATGCAAGGAAGCTAGAGAAGAAGAATGTCTTCAAGGGAATACATACCAATAAATTGTTTATGGGGATCATTGCAGTGACAATTGTTCTTCAGGTCGTGATGGTGGAGGTTCTCAAGAAATTTGCAGATACAGAGAGGTTGAACTGGGGACAATGGGGTGTATGCATTGGGATTGCTGTCATCTCTTGGCCAATTGGTTGGCTAGTCAAGTCTATACCTGTTCCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

294

Amino Acids

32.45

Weight (kDa)

9.05

Isoelectric Point (pI)

19.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_C PF00689 120 - 289 4.3e-50 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 481
AccI GTMKAC 1 cut(s) 264
AclWI GGATC 1 cut(s) 728
AcoI YGGCCR 1 cut(s) 842
AcsI RAATTY 2 cut(s) 18, 773
AcuI CTGAAG 2 cut(s) 213, 728
AfaI GTAC 1 cut(s) 533
AfiI CCNNNNNNNGG 1 cut(s) 470
AgsI TTSAA 8 cut(s) 76, 170, 377, 619, 646, 655, 688, 796
AjnI CCWGG 1 cut(s) 636
AleI CACNNNNGTG 1 cut(s) 626
AluBI AGCT 6 cut(s) 55, 143, 195, 317, 416, 667
AluI AGCT 6 cut(s) 55, 143, 195, 317, 416, 667
Alw21I GWGCWC 1 cut(s) 418
AlwI GGATC 1 cut(s) 728
AoxI GGCC 1 cut(s) 842
ApeKI GCWGC 2 cut(s) 314, 338
ApoI RAATTY 2 cut(s) 18, 773
Asp700I GAANNNNTTC 1 cut(s) 683
AsuC2I CCSGG 1 cut(s) 566
AsuHPI GGTGA 2 cut(s) 119, 292
BalI TGGCCA 1 cut(s) 844
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 1 cut(s) 418
BbsI GAAGAC 2 cut(s) 30, 676
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 326, 350
BccI CCATC 3 cut(s) 22, 104, 748
BceAI ACGGC 1 cut(s) 495
BciT130I CCWGG 1 cut(s) 638
BclI TGATCA 3 cut(s) 207, 324, 396
BcnI CCSGG 1 cut(s) 566
BfaI CTAG 2 cut(s) 668, 857
BfmI CTRYAG 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 341
BisI GCNGC 2 cut(s) 315, 339
BlsI GCNGC 2 cut(s) 316, 340
Bme1390I CCNGG 2 cut(s) 566, 638
BmiI GGNNCC 1 cut(s) 483
BmrFI CCNGG 2 cut(s) 566, 638
BmrI ACTGGG 1 cut(s) 808
BmsI GCATC 1 cut(s) 112
BmuI ACTGGG 1 cut(s) 808
BpiI GAAGAC 2 cut(s) 30, 676
BpuEI CTTGAG 2 cut(s) 507, 752
BpuMI CCSGG 1 cut(s) 566
BsaJI CCNNGG 3 cut(s) 159, 186, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 467, 803
Bse3DI GCAATG 2 cut(s) 74, 723
BseBI CCWGG 1 cut(s) 638
BseDI CCNNGG 3 cut(s) 159, 186, 565
BseGI GGATG 2 cut(s) 220, 285
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 2 cut(s) 74, 723
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 2 cut(s) 467, 803
BseXI GCAGC 2 cut(s) 326, 350
BshFI GGCC 1 cut(s) 844
BshNI GGYRCC 1 cut(s) 481
BsiHKAI GWGCWC 1 cut(s) 418
BsiSI CCGG 1 cut(s) 565
BslFI GGGAC 2 cut(s) 344, 816
BslI CCNNNNNNNGG 1 cut(s) 470
BsmFI GGGAC 2 cut(s) 344, 816
BsnI GGCC 1 cut(s) 844
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 6 cut(s) 207, 324, 396, 552, 612, 720
Bsp19I CCATGG 1 cut(s) 159
BspANI GGCC 1 cut(s) 844
BspCNI CTCAG 1 cut(s) 209
BspLI GGNNCC 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 341
BspPI GGATC 1 cut(s) 728
BspT107I GGYRCC 1 cut(s) 481
BsrDI GCAATG 2 cut(s) 74, 723
BsrI ACTGG 2 cut(s) 467, 803
BssECI CCNNGG 3 cut(s) 159, 186, 565
BssMI GATC 6 cut(s) 207, 324, 396, 552, 612, 720
BssT1I CCWWGG 2 cut(s) 159, 186
Bst2UI CCWGG 1 cut(s) 638
Bst4CI ACNGT 4 cut(s) 241, 304, 531, 544
BstDEI CTNAG 1 cut(s) 196
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 2 cut(s) 220, 285
BstKTI GATC 6 cut(s) 210, 327, 399, 555, 615, 723
BstMBI GATC 6 cut(s) 207, 324, 396, 552, 612, 720
BstMWI GCNNNNNNNGC 2 cut(s) 233, 347
BstNI CCWGG 1 cut(s) 638
BstSCI CCNGG 2 cut(s) 564, 636
BstSFI CTRYAG 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 326, 350
BstV2I GAAGAC 2 cut(s) 30, 676
BstXI CCANNNNNNTGG 1 cut(s) 852
BsuRI GGCC 1 cut(s) 844
BtgI CCRYGG 1 cut(s) 159
BtsCI GGATG 2 cut(s) 220, 285
BtsI GCAGTG 2 cut(s) 105, 735
BtsIMutI CAGTG 5 cut(s) 105, 474, 527, 595, 735
BveI ACCTGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 532
CspCI CAANNNNNGTGG 2 cut(s) 365, 400
CviAII CATG 5 cut(s) 89, 160, 211, 400, 502
CviJI RGCY 9 cut(s) 55, 143, 195, 317, 416, 463, 667, 844, 856
CviKI_1 RGCY 9 cut(s) 55, 143, 195, 317, 416, 463, 667, 844, 856
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 1 cut(s) 196
DpnI GATC 6 cut(s) 209, 326, 398, 554, 614, 722
DpnII GATC 6 cut(s) 207, 324, 396, 552, 612, 720
EaeI YGGCCR 1 cut(s) 842
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 159, 186
Eco24I GRGCYC 1 cut(s) 418
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 2 cut(s) 213, 728
EcoICRI GAGCTC 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 636
EcoT14I CCWWGG 2 cut(s) 159, 186
EcoT22I ATGCAT 1 cut(s) 821
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 2 cut(s) 159, 186
FaeI CATG 5 cut(s) 92, 163, 214, 403, 505
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 2 cut(s) 344, 816
FatI CATG 5 cut(s) 88, 159, 210, 399, 501
FbaI TGATCA 3 cut(s) 207, 324, 396
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 2 cut(s) 315, 339
FokI GGATG 2 cut(s) 227, 272
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 315, 339
FspBI CTAG 2 cut(s) 668, 857
GluI GCNGC 2 cut(s) 315, 339
HaeIII GGCC 1 cut(s) 844
HapII CCGG 1 cut(s) 565
Hin1II CATG 5 cut(s) 92, 163, 214, 403, 505
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 2 cut(s) 166, 511
HpaII CCGG 1 cut(s) 565
HphI GGTGA 2 cut(s) 119, 292
Hpy166II GTNNAC 2 cut(s) 265, 391
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 5 cut(s) 277, 550, 751, 769, 878
Hpy8I GTNNAC 2 cut(s) 265, 391
HpyAV CCTTC 1 cut(s) 468
HpyCH4III ACNGT 4 cut(s) 241, 304, 531, 544
HpyCH4V TGCA 9 cut(s) 67, 98, 125, 295, 314, 659, 728, 779, 819
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 347
HpyF3I CTNAG 1 cut(s) 196
Hsp92II CATG 5 cut(s) 92, 163, 214, 403, 505
Ksp22I TGATCA 3 cut(s) 207, 324, 396
Kzo9I GATC 6 cut(s) 207, 324, 396, 552, 612, 720
LmnI GCTCC 1 cut(s) 413
LpnPI CCDG 9 cut(s) 141, 290, 336, 480, 578, 623, 650, 731, 784
Lsp1109I GCAGC 2 cut(s) 326, 350
LweI GCATC 1 cut(s) 112
MaeI CTAG 2 cut(s) 668, 857
MaeIII GTNAC 2 cut(s) 100, 730
MalI GATC 6 cut(s) 209, 326, 398, 554, 614, 722
MboI GATC 6 cut(s) 207, 324, 396, 552, 612, 720
MboII GAAGA 7 cut(s) 30, 267, 607, 676, 685, 688, 734
MfeI CAATTG 3 cut(s) 290, 735, 846
MhlI GDGCHC 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 844
MluCI AATT 8 cut(s) 18, 290, 377, 560, 707, 735, 773, 846
MluNI TGGCCA 1 cut(s) 844
MnlI CCTC 6 cut(s) 495, 501, 531, 557, 754, 783
Mox20I TGGCCA 1 cut(s) 844
Mph1103I ATGCAT 1 cut(s) 821
MroXI GAANNNNTTC 1 cut(s) 683
MscI TGGCCA 1 cut(s) 844
MseI TTAA 2 cut(s) 134, 365
MslI CAYNNNNRTG 2 cut(s) 626, 728
Msp20I TGGCCA 1 cut(s) 844
MspI CCGG 1 cut(s) 565
MspR9I CCNGG 2 cut(s) 566, 638
MunI CAATTG 3 cut(s) 290, 735, 846
MvaI CCWGG 1 cut(s) 638
MwoI GCNNNNNNNGC 2 cut(s) 233, 347
NciI CCSGG 1 cut(s) 566
NcoI CCATGG 1 cut(s) 159
NdeII GATC 6 cut(s) 207, 324, 396, 552, 612, 720
NlaIII CATG 5 cut(s) 92, 163, 214, 403, 505
NlaIV GGNNCC 1 cut(s) 483
NmuCI GTSAC 2 cut(s) 100, 730
NsiI ATGCAT 1 cut(s) 821
OliI CACNNNNGTG 1 cut(s) 626
PdmI GAANNNNTTC 1 cut(s) 683
PfeI GAWTC 2 cut(s) 166, 511
PkrI GCNGC 2 cut(s) 316, 340
Psp124BI GAGCTC 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 636
PspGI CCWGG 1 cut(s) 636
PspN4I GGNNCC 1 cut(s) 483
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
RseI CAYNNNNRTG 2 cut(s) 626, 728
SacI GAGCTC 1 cut(s) 418
SaqAI TTAA 2 cut(s) 134, 365
SatI GCNGC 2 cut(s) 315, 339
Sau3AI GATC 6 cut(s) 207, 324, 396, 552, 612, 720
ScrFI CCNGG 2 cut(s) 566, 638
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 1 cut(s) 112
SfcI CTRYAG 1 cut(s) 237
SmiMI CAYNNNNRTG 2 cut(s) 626, 728
SmlI CTYRAG 2 cut(s) 522, 767
SmoI CTYRAG 2 cut(s) 522, 767
Sse9I AATT 8 cut(s) 18, 290, 377, 560, 707, 735, 773, 846
SspMI CTAG 2 cut(s) 668, 857
SstI GAGCTC 1 cut(s) 418
StyD4I CCNGG 2 cut(s) 564, 636
StyI CCWWGG 2 cut(s) 159, 186
TaaI ACNGT 4 cut(s) 241, 304, 531, 544
TasI AATT 8 cut(s) 18, 290, 377, 560, 707, 735, 773, 846
TfiI GAWTC 2 cut(s) 166, 511
Tru1I TTAA 2 cut(s) 134, 365
Tru9I TTAA 2 cut(s) 134, 365
TscAI CASTG 5 cut(s) 105, 474, 534, 595, 735
TseFI GTSAC 2 cut(s) 100, 730
TseI GCWGC 2 cut(s) 314, 338
Tsp45I GTSAC 2 cut(s) 100, 730
TspDTI ATGAA 1 cut(s) 274
TspRI CASTG 5 cut(s) 105, 474, 534, 595, 735
XapI RAATTY 2 cut(s) 18, 773
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 683
XspI CTAG 2 cut(s) 668, 857
Zsp2I ATGCAT 1 cut(s) 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.