FvH4_4g07450

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004280
Physical Location & Seq
Reverse (-)
6813493 .. 6819535
6043 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07450.t1

Sequence Viewer

Length: 1179 bp
ATGAAGACAGGTCAGCCTTGGCGAATAAACATGGGTGACATTCCTATATTTTCTGGGGCTCGCCATCGGCCACCTTTGAAGAGGCCGTTGTGGATCATTGTCTTGGTGTCCTTGGTTAGCATCTTTGTTATTTGTGCTTTTCTCTATCCACCGCAAAGCAGCGCCGCTTGTTACATATTTTCGTCAAGAGGTTGTAAGGCTATTTCCGATTGGCTTCCACCTGCTCCAGCAAGGGAATATAATGATGATGAGATTGCATCCCGGGTAGTGATAAAAGAAATTTTGAGTGCACCTCCTGTTCAATCAAAAAACCCCAAACTAGCTTTCATGTTCCTTACACCTAGTGCAGTGCCGTTTGAGAAGCTGTGGGATAAATTTTTCCATGGTCATGAGGGAAAGTTCTCTGTTTATGTGCATGCATCTAAAGAAAAGGCAGTTCATCTGAGCCGTTACTTTGCTAATAGAGAGATACGCAGCGACCAGGTGATTTGGGGAAAAATTTCCATGGTAGATGCAGAGAGACGATTATTGGCAAATGCCCTTCAAGATCCTGATAACCAGCACTTTGTGTTACTTTCTGAAAGTTGTGTACCGCTGTATACATTTGACTATATCTATGACTATCTGATGAATACCAATATAAGCTACGTTGACTGCTTTGAGGATCCTGGCCCACATGGAAATGGCAGGTACTCAGAACACATGTTGCCTGAAATTGAGATGAAAGACTTCAGAAAGGGTGCACAGTGGTTCTCTGTCAAGCGTCAGCATGCTGTTATAATAATGGCCGATAATCTTTACTACACAAAATTCCGGGATTACTGCAAGCCAGGTTTGGAGGGGCGCAATTGCATTGCTGATGAACATTACTTGCCAACCTTTTTTAATATAATTGATCCTGGTGGAATTGCAAACTGGTCTGTAACACATGTTGATTGGTCTGAAAGAAAGTGGCACCCTAAACTATACAGGGCTGGGGATATTAGTTACGAGCTTCTGAGAAATATTACGTCAATTGATGTGAGCATTCATGTAACCAGTGATGCTAAGAAAGTAGTGCAAAGATGGCCTTGCTTATGGAATGGTCAGCAACGGCCGTGTTACTTGTTTGCAAGGAAGTTTCATCCAGAGAGTCTCAGTAACTTGTTGCACCTTTTTGCCAATTATACAACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

393

Amino Acids

45.38

Weight (kDa)

8.59

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 107 - 336 1.4e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 779
AarI CACCTGC 1 cut(s) 229
Acc36I ACCTGC 2 cut(s) 229, 678
AccB1I GGYRCC 1 cut(s) 954
AccI GTMKAC 1 cut(s) 599
AciI CCGC 3 cut(s) 152, 165, 593
AclWI GGATC 5 cut(s) 101, 542, 659, 672, 890
AcoI YGGCCR 3 cut(s) 68, 786, 1094
AcsI RAATTY 4 cut(s) 279, 374, 498, 809
AcuI CTGAAG 1 cut(s) 715
AdeI CACNNNGTG 2 cut(s) 344, 568
AfaI GTAC 2 cut(s) 591, 692
AflIII ACRYGT 2 cut(s) 702, 928
AgsI TTSAA 3 cut(s) 79, 302, 545
AjnI CCWGG 4 cut(s) 480, 667, 829, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 4 cut(s) 323, 364, 645, 994
AluI AGCT 4 cut(s) 323, 364, 645, 994
Alw21I GWGCWC 2 cut(s) 292, 745
Alw26I GTCTC 2 cut(s) 514, 1139
Alw44I GTGCAC 2 cut(s) 288, 741
AlwI GGATC 5 cut(s) 101, 542, 659, 672, 890
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 6 cut(s) 68, 83, 670, 786, 1067, 1094
ApaLI GTGCAC 2 cut(s) 288, 741
ApeKI GCWGC 2 cut(s) 159, 474
ApoI RAATTY 4 cut(s) 279, 374, 498, 809
Asp700I GAANNNNTTC 2 cut(s) 499, 728
AspLEI GCGC 2 cut(s) 164, 846
AspS9I GGNCC 1 cut(s) 671
AsuC2I CCSGG 3 cut(s) 262, 263, 815
AsuHPI GGTGA 2 cut(s) 47, 496
AvaI CYCGRG 1 cut(s) 261
BaeGI GKGCMC 2 cut(s) 292, 745
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 292, 745
BbvI GCAGC 2 cut(s) 171, 486
BccI CCATC 2 cut(s) 72, 1059
BceAI ACGGC 5 cut(s) 70, 337, 432, 1081, 1109
BciT130I CCWGG 4 cut(s) 482, 669, 831, 900
BcnI CCSGG 3 cut(s) 262, 263, 815
BcoDI GTCTC 2 cut(s) 514, 1139
BfaI CTAG 2 cut(s) 320, 342
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 2 cut(s) 229, 678
BisI GCNGC 3 cut(s) 160, 165, 475
BlsI GCNGC 3 cut(s) 161, 166, 476
Bme1390I CCNGG 7 cut(s) 262, 263, 482, 669, 815, 831, 900
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 2 cut(s) 666, 956
BmrFI CCNGG 7 cut(s) 262, 263, 482, 669, 815, 831, 900
BmsI GCATC 5 cut(s) 129, 266, 428, 502, 1033
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 1 cut(s) 210
BpuMI CCSGG 3 cut(s) 262, 263, 815
BsaJI CCNNGG 5 cut(s) 17, 111, 261, 382, 504
Bse1I ACTGG 2 cut(s) 920, 1038
Bse3DI GCAATG 1 cut(s) 852
BseBI CCWGG 4 cut(s) 482, 669, 831, 900
BseDI CCNNGG 5 cut(s) 17, 111, 261, 382, 504
BseGI GGATG 2 cut(s) 257, 1123
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 4 cut(s) 434, 708, 989, 1150
BseNI ACTGG 2 cut(s) 920, 1038
BseSI GKGCMC 2 cut(s) 292, 745
BseX3I CGGCCG 1 cut(s) 1094
BseXI GCAGC 2 cut(s) 171, 486
BseYI CCCAGC 1 cut(s) 974
BsgI GTGCAG 1 cut(s) 366
Bsh1285I CGRYCG 1 cut(s) 1097
BshFI GGCC 6 cut(s) 70, 85, 672, 788, 1069, 1096
BshNI GGYRCC 1 cut(s) 954
BsiEI CGRYCG 1 cut(s) 1097
BsiHKAI GWGCWC 2 cut(s) 292, 745
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 262, 814
BsmAI GTCTC 2 cut(s) 514, 1139
BsmBI CGTCTC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 1026
BsnI GGCC 6 cut(s) 70, 85, 672, 788, 1069, 1096
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 61, 292, 745
Bsp143I GATC 4 cut(s) 93, 547, 664, 895
Bsp19I CCATGG 2 cut(s) 382, 504
BspACI CCGC 3 cut(s) 152, 165, 593
BspANI GGCC 6 cut(s) 70, 85, 672, 788, 1069, 1096
BspCNI CTCAG 4 cut(s) 435, 707, 990, 1149
BspHI TCATGA 1 cut(s) 388
BspLI GGNNCC 2 cut(s) 666, 956
BspMI ACCTGC 2 cut(s) 229, 678
BspPI GGATC 5 cut(s) 101, 542, 659, 672, 890
BspT107I GGYRCC 1 cut(s) 954
BsrDI GCAATG 1 cut(s) 852
BsrI ACTGG 2 cut(s) 920, 1038
BssECI CCNNGG 5 cut(s) 17, 111, 261, 382, 504
BssMI GATC 4 cut(s) 93, 547, 664, 895
BssNAI GTATAC 1 cut(s) 600
BssT1I CCWWGG 4 cut(s) 17, 111, 382, 504
Bst1107I GTATAC 1 cut(s) 600
Bst2UI CCWGG 4 cut(s) 482, 669, 831, 900
Bst4CI ACNGT 2 cut(s) 747, 1174
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 61, 417, 771, 827
BstDEI CTNAG 5 cut(s) 443, 694, 998, 1047, 1136
BstDSI CCRYGG 2 cut(s) 382, 504
BstF5I GGATG 2 cut(s) 257, 1123
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 846
BstKTI GATC 4 cut(s) 96, 550, 667, 898
BstMAI GTCTC 2 cut(s) 514, 1139
BstMBI GATC 4 cut(s) 93, 547, 664, 895
BstMCI CGRYCG 1 cut(s) 1097
BstMWI GCNNNNNNNGC 1 cut(s) 1066
BstNI CCWGG 4 cut(s) 482, 669, 831, 900
BstNSI RCATGY 4 cut(s) 419, 706, 773, 932
BstSCI CCNGG 7 cut(s) 260, 261, 480, 667, 813, 829, 898
BstSLI GKGCMC 2 cut(s) 292, 745
BstV1I GCAGC 2 cut(s) 171, 486
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 547, 664
BstYI RGATCY 2 cut(s) 547, 664
BstZ17I GTATAC 1 cut(s) 600
BstZI CGGCCG 1 cut(s) 1094
BsuRI GGCC 6 cut(s) 70, 85, 672, 788, 1069, 1096
BtgI CCRYGG 2 cut(s) 382, 504
BtsCI GGATG 2 cut(s) 257, 1123
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 3 cut(s) 354, 752, 1045
BveI ACCTGC 2 cut(s) 229, 678
Cac8I GCNNGC 4 cut(s) 61, 417, 771, 827
CciI TCATGA 1 cut(s) 388
CfoI GCGC 2 cut(s) 164, 846
Cfr13I GGNCC 1 cut(s) 671
Cfr9I CCCGGG 1 cut(s) 261
CseI GACGC 1 cut(s) 752
CsiI ACCWGGT 1 cut(s) 480
Csp6I GTAC 2 cut(s) 590, 691
CviQI GTAC 2 cut(s) 590, 691
DdeI CTNAG 5 cut(s) 443, 694, 998, 1047, 1136
DpnI GATC 4 cut(s) 95, 549, 666, 897
DpnII GATC 4 cut(s) 93, 547, 664, 895
DraIII CACNNNGTG 2 cut(s) 344, 568
EaeI YGGCCR 3 cut(s) 68, 786, 1094
EagI CGGCCG 1 cut(s) 1094
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
EclXI CGGCCG 1 cut(s) 1094
Eco130I CCWWGG 4 cut(s) 17, 111, 382, 504
Eco24I GRGCYC 1 cut(s) 61
Eco52I CGGCCG 1 cut(s) 1094
Eco57I CTGAAG 1 cut(s) 715
Eco88I CYCGRG 1 cut(s) 261
EcoRII CCWGG 4 cut(s) 480, 667, 829, 898
EcoT14I CCWWGG 4 cut(s) 17, 111, 382, 504
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 4 cut(s) 17, 111, 382, 504
Esp3I CGTCTC 1 cut(s) 514
FalI AAGNNNNNCTT 2 cut(s) 1054, 1086
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 3 cut(s) 160, 165, 475
FokI GGATG 2 cut(s) 244, 1110
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 3 cut(s) 160, 165, 475
FspBI CTAG 2 cut(s) 320, 342
GlaI GCGC 2 cut(s) 163, 845
GluI GCNGC 3 cut(s) 160, 165, 475
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 6 cut(s) 70, 85, 672, 788, 1069, 1096
HapII CCGG 2 cut(s) 262, 814
HgaI GACGC 1 cut(s) 752
HhaI GCGC 2 cut(s) 164, 846
Hin6I GCGC 2 cut(s) 162, 844
HinP1I GCGC 2 cut(s) 162, 844
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 1 cut(s) 1132
HpaII CCGG 2 cut(s) 262, 814
HphI GGTGA 2 cut(s) 47, 496
Hpy166II GTNNAC 5 cut(s) 290, 590, 600, 652, 743
Hpy188I TCNGA 8 cut(s) 208, 444, 580, 627, 697, 734, 943, 999
Hpy188III TCNNGA 5 cut(s) 186, 389, 545, 551, 1127
Hpy8I GTNNAC 5 cut(s) 290, 590, 600, 652, 743
HpyAV CCTTC 1 cut(s) 551
HpyCH4III ACNGT 2 cut(s) 747, 1174
HpyCH4IV ACGT 2 cut(s) 648, 1010
HpyF10VI GCNNNNNNNGC 1 cut(s) 1066
HpyF3I CTNAG 5 cut(s) 443, 694, 998, 1047, 1136
HpySE526I ACGT 2 cut(s) 648, 1010
HspAI GCGC 2 cut(s) 162, 844
Kzo9I GATC 4 cut(s) 93, 547, 664, 895
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 2 cut(s) 171, 486
LweI GCATC 5 cut(s) 129, 266, 428, 502, 1033
MabI ACCWGGT 1 cut(s) 480
MaeI CTAG 2 cut(s) 320, 342
MaeII ACGT 2 cut(s) 648, 1010
MaeIII GTNAC 9 cut(s) 35, 170, 449, 570, 922, 986, 1033, 1100, 1139
MalI GATC 4 cut(s) 95, 549, 666, 897
MboI GATC 4 cut(s) 93, 547, 664, 895
MboII GAAGA 2 cut(s) 16, 91
MfeI CAATTG 2 cut(s) 847, 1014
MflI RGATCY 2 cut(s) 547, 664
MhlI GDGCHC 3 cut(s) 61, 292, 745
MlyI GAGTC 1 cut(s) 1141
MnlI CCTC 6 cut(s) 75, 182, 303, 385, 655, 832
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 2 cut(s) 499, 728
MseI TTAA 1 cut(s) 885
MslI CAYNNNNRTG 2 cut(s) 387, 681
MspA1I CMGCKG 1 cut(s) 595
MspI CCGG 2 cut(s) 262, 814
MspR9I CCNGG 7 cut(s) 262, 263, 482, 669, 815, 831, 900
MunI CAATTG 2 cut(s) 847, 1014
Mva1269I GAATGC 1 cut(s) 1026
MvaI CCWGG 4 cut(s) 482, 669, 831, 900
MwoI GCNNNNNNNGC 1 cut(s) 1066
NciI CCSGG 3 cut(s) 262, 263, 815
NcoI CCATGG 2 cut(s) 382, 504
NdeII GATC 4 cut(s) 93, 547, 664, 895
NlaIV GGNNCC 2 cut(s) 666, 956
NmuCI GTSAC 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 4 cut(s) 419, 706, 773, 932
PaeI GCATGC 2 cut(s) 419, 773
PagI TCATGA 1 cut(s) 388
PaqCI CACCTGC 1 cut(s) 229
PciI ACATGT 2 cut(s) 702, 928
PctI GAATGC 1 cut(s) 1026
PdmI GAANNNNTTC 2 cut(s) 499, 728
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 3 cut(s) 161, 166, 476
PleI GAGTC 1 cut(s) 1140
PpsI GAGTC 1 cut(s) 1140
PscI ACATGT 2 cut(s) 702, 928
PsiI TTATAA 1 cut(s) 779
Psp6I CCWGG 4 cut(s) 480, 667, 829, 898
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 4 cut(s) 480, 667, 829, 898
PspN4I GGNNCC 2 cut(s) 666, 956
PspPI GGNCC 1 cut(s) 671
PsuI RGATCY 2 cut(s) 547, 664
RsaI GTAC 2 cut(s) 591, 692
RsaNI GTAC 2 cut(s) 590, 691
RseI CAYNNNNRTG 2 cut(s) 387, 681
SaqAI TTAA 1 cut(s) 885
SatI GCNGC 3 cut(s) 160, 165, 475
Sau3AI GATC 4 cut(s) 93, 547, 664, 895
Sau96I GGNCC 1 cut(s) 671
SchI GAGTC 1 cut(s) 1141
ScrFI CCNGG 7 cut(s) 262, 263, 482, 669, 815, 831, 900
SduI GDGCHC 3 cut(s) 61, 292, 745
SexAI ACCWGGT 1 cut(s) 480
SfaNI GCATC 5 cut(s) 129, 266, 428, 502, 1033
SmaI CCCGGG 1 cut(s) 263
SmiMI CAYNNNNRTG 2 cut(s) 387, 681
SphI GCATGC 2 cut(s) 419, 773
SsiI CCGC 3 cut(s) 152, 165, 593
SspI AATATT 1 cut(s) 1006
SspMI CTAG 2 cut(s) 320, 342
StyD4I CCNGG 7 cut(s) 260, 261, 480, 667, 813, 829, 898
StyI CCWWGG 4 cut(s) 17, 111, 382, 504
TaaI ACNGT 2 cut(s) 747, 1174
TaiI ACGT 2 cut(s) 651, 1013
TauI GCSGC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 885
Tru9I TTAA 1 cut(s) 885
TscAI CASTG 3 cut(s) 354, 752, 1045
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 2 cut(s) 159, 474
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 8 cut(s) 17, 316, 428, 644, 737, 876, 1019, 1112
TspMI CCCGGG 1 cut(s) 261
TspRI CASTG 3 cut(s) 354, 752, 1045
VneI GTGCAC 2 cut(s) 288, 741
XapI RAATTY 4 cut(s) 279, 374, 498, 809
XceI RCATGY 4 cut(s) 419, 706, 773, 932
XmaI CCCGGG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 599
XmnI GAANNNNTTC 2 cut(s) 499, 728
XspI CTAG 2 cut(s) 320, 342
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.