AT2G20060

Ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002609
Physical Location & Seq
Forward (+)
8658981 .. 8661558
2578 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G20060.1

Sequence Viewer

Length: 903 bp
ATGGCTGCTTCAATTTCAAGAAGGATTTTTCGCACTTTTGGATCCTTTTCAGCTCTAGGACGTTCCTCTTCACCAAATTTGTCTGCGAATACATCTGGAAATGGTTTAGCTTCTCTTACTAATGATCTAAAGTATGGAATGGCTTTACTTTCCAACCGAAAGCTCTCCACTTCTATTTTGACTCCTGATGATTCATTTCCTTCTGATTTACTTACCAAAAAGACTGTGCTTACACCAGACCGAACCATAGGACAATATCAAGACTTGGTCATTCCAGTGACTAATTTTCAGAACGAGGAAAAGGGTTTCATGGTTTTAGCAGGTGATGTCTTTGATGTTCCAATAAGGAAAGATATCATTCACAATGTCGTGCGATGGCAGCTTGCGAAACGCCAGCAGGGAACTCATTCGACCAAAACGTTAAGTGAGGTTAGTGGAACTGGTAGAAAGCCATGGAATCAGAAGGGTACAGGTCGAGCTAGACATGGTACACTACGTGGTCCGCAGTTCCGAGGTGGTTGTGTAATGCATGGACCCAAACCAAGAAGCCATGCCATAAAAATGAATAAGCAGGTTCGACGGCTTGGACTGAAAATAGCATTGACAGCTCGAGCCGCAGAAGGAAAACTCCTCGTGTTTGATGATTTGGGATTGCCTACGCATAAAACAAAGAACATTGTGAACTATTACAACCAAATGGAGAACACAAAGAAAGTCCTTGTTGTAGAAGGTGGTCCTATTGATGAAAAGCTGAAGCTAGCCACCCAAAATCTACACTATGTCAACATAATTCCATCAATAGGGCTAAATGTTTACAGCATTCTGCTACACGATACGCTTCTGATGTCCCGTGATGCTGTGAATAAGATCGTTGAGCGTATGCACACTCCTATTAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

300

Amino Acids

33.26

Weight (kDa)

10.3

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 106 - 293 3.6e-67 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 311
Acc36I ACCTGC 2 cut(s) 311, 562
AciI CCGC 2 cut(s) 503, 615
AclI AACGTT 1 cut(s) 419
AclWI GGATC 2 cut(s) 36, 49
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 1 cut(s) 773
AdeI CACNNNGTG 1 cut(s) 497
AfaI GTAC 2 cut(s) 469, 490
AfiI CCNNNNNNNGG 1 cut(s) 800
AgsI TTSAA 2 cut(s) 12, 18
AluBI AGCT 8 cut(s) 53, 110, 163, 382, 479, 608, 751, 757
AluI AGCT 8 cut(s) 53, 110, 163, 382, 479, 608, 751, 757
AlwI GGATC 2 cut(s) 36, 49
Ama87I CYCGRG 1 cut(s) 609
ApeKI GCWGC 2 cut(s) 5, 379
ApoI RAATTY 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 406
AspS9I GGNCC 3 cut(s) 500, 533, 734
AsuHPI GGTGA 2 cut(s) 63, 335
AsuNHI GCTAGC 1 cut(s) 757
AvaI CYCGRG 1 cut(s) 609
AvaII GGWCC 3 cut(s) 500, 533, 734
BamHI GGATCC 1 cut(s) 41
BauI CACGAG 1 cut(s) 632
BbvI GCAGC 1 cut(s) 391
BccI CCATC 2 cut(s) 369, 802
BceAI ACGGC 1 cut(s) 596
BfaI CTAG 3 cut(s) 56, 480, 758
BfuAI ACCTGC 2 cut(s) 311, 562
BisI GCNGC 3 cut(s) 6, 380, 615
BlsI GCNGC 3 cut(s) 7, 381, 616
Bme18I GGWCC 3 cut(s) 500, 533, 734
BmeT110I CYCGRG 1 cut(s) 609
BmgT120I GGNCC 3 cut(s) 500, 533, 734
BmiI GGNNCC 2 cut(s) 43, 535
BmsI GCATC 1 cut(s) 844
BmtI GCTAGC 1 cut(s) 761
BsaAI YACGTR 1 cut(s) 497
BsaJI CCNNGG 2 cut(s) 452, 511
Bsc4I CCNNNNNNNGG 1 cut(s) 800
Bse1I ACTGG 2 cut(s) 275, 445
BseDI CCNNGG 2 cut(s) 452, 511
BseLI CCNNNNNNNGG 1 cut(s) 800
BseNI ACTGG 2 cut(s) 275, 445
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 609
BslFI GGGAC 1 cut(s) 832
BslI CCNNNNNNNGG 1 cut(s) 800
BsmFI GGGAC 1 cut(s) 832
BsmI GAATGC 1 cut(s) 819
BsoBI CYCGRG 1 cut(s) 609
Bsp143I GATC 3 cut(s) 41, 124, 867
Bsp19I CCATGG 1 cut(s) 452
BspACI CCGC 2 cut(s) 503, 615
BspLI GGNNCC 2 cut(s) 43, 535
BspMI ACCTGC 2 cut(s) 311, 562
BspOI GCTAGC 1 cut(s) 761
BspPI GGATC 2 cut(s) 36, 49
BsrI ACTGG 2 cut(s) 275, 445
BssECI CCNNGG 2 cut(s) 452, 511
BssMI GATC 3 cut(s) 41, 124, 867
BssSI CACGAG 1 cut(s) 632
BssT1I CCWWGG 1 cut(s) 452
Bst2BI CACGAG 1 cut(s) 632
Bst4CI ACNGT 2 cut(s) 226, 899
Bst6I CTCTTC 1 cut(s) 73
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 3 cut(s) 384, 395, 759
BstDSI CCRYGG 1 cut(s) 452
BstKTI GATC 3 cut(s) 44, 127, 870
BstMBI GATC 3 cut(s) 41, 124, 867
BstMWI GCNNNNNNNGC 3 cut(s) 379, 605, 614
BstV1I GCAGC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 452
BtgZI GCGATG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 282
BveI ACCTGC 2 cut(s) 311, 562
Cac8I GCNNGC 3 cut(s) 384, 395, 759
Cfr13I GGNCC 3 cut(s) 500, 533, 734
Csp6I GTAC 2 cut(s) 468, 489
CviAII CATG 5 cut(s) 310, 453, 485, 530, 551
CviQI GTAC 2 cut(s) 468, 489
DpnI GATC 3 cut(s) 43, 126, 869
DpnII GATC 3 cut(s) 41, 124, 867
DraIII CACNNNGTG 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 452
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 3 cut(s) 500, 533, 734
Eco57I CTGAAG 1 cut(s) 773
Eco88I CYCGRG 1 cut(s) 609
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 1 cut(s) 452
EcoT22I ATGCAT 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 452
FaeI CATG 5 cut(s) 313, 456, 488, 533, 554
FaqI GGGAC 1 cut(s) 832
FatI CATG 5 cut(s) 309, 452, 484, 529, 550
Fnu4HI GCNGC 3 cut(s) 6, 380, 615
Fsp4HI GCNGC 3 cut(s) 6, 380, 615
FspBI CTAG 3 cut(s) 56, 480, 758
GluI GCNGC 3 cut(s) 6, 380, 615
Hin1II CATG 5 cut(s) 313, 456, 488, 533, 554
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HinfI GANTC 3 cut(s) 181, 191, 457
HphI GGTGA 2 cut(s) 63, 335
Hpy166II GTNNAC 4 cut(s) 491, 682, 784, 814
Hpy188I TCNGA 5 cut(s) 205, 291, 462, 512, 843
Hpy188III TCNNGA 4 cut(s) 18, 96, 185, 260
Hpy8I GTNNAC 4 cut(s) 491, 682, 784, 814
Hpy99I CGWCG 1 cut(s) 582
HpyAV CCTTC 5 cut(s) 15, 210, 457, 614, 722
HpyCH4III ACNGT 2 cut(s) 226, 899
HpyCH4IV ACGT 3 cut(s) 61, 419, 496
HpyCH4V TGCA 2 cut(s) 529, 883
HpyF10VI GCNNNNNNNGC 3 cut(s) 379, 605, 614
HpySE526I ACGT 3 cut(s) 61, 419, 496
Hsp92II CATG 5 cut(s) 313, 456, 488, 533, 554
Kzo9I GATC 3 cut(s) 41, 124, 867
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 1 cut(s) 844
MaeI CTAG 3 cut(s) 56, 480, 758
MaeII ACGT 3 cut(s) 61, 419, 496
MaeIII GTNAC 1 cut(s) 277
MalI GATC 3 cut(s) 43, 126, 869
MboI GATC 3 cut(s) 41, 124, 867
MboII GAAGA 1 cut(s) 60
MflI RGATCY 1 cut(s) 41
MluCI AATT 4 cut(s) 12, 76, 283, 789
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 5 cut(s) 76, 289, 421, 506, 641
Mph1103I ATGCAT 1 cut(s) 531
MroXI GAANNNNTTC 1 cut(s) 406
MseI TTAA 2 cut(s) 422, 894
MslI CAYNNNNRTG 2 cut(s) 275, 560
Mva1269I GAATGC 1 cut(s) 819
MwoI GCNNNNNNNGC 3 cut(s) 379, 605, 614
NcoI CCATGG 1 cut(s) 452
NdeII GATC 3 cut(s) 41, 124, 867
NheI GCTAGC 1 cut(s) 757
NlaIII CATG 5 cut(s) 313, 456, 488, 533, 554
NlaIV GGNNCC 2 cut(s) 43, 535
NmuCI GTSAC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 531
PaeR7I CTCGAG 1 cut(s) 609
PaqCI CACCTGC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PctI GAATGC 1 cut(s) 819
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 2 cut(s) 191, 457
PflFI GACNNNGTC 1 cut(s) 266
PkrI GCNGC 3 cut(s) 7, 381, 616
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 497
Psp1406I AACGTT 1 cut(s) 419
PspN4I GGNNCC 2 cut(s) 43, 535
PspPI GGNCC 3 cut(s) 500, 533, 734
PspXI VCTCGAGB 1 cut(s) 609
PsuI RGATCY 1 cut(s) 41
PsyI GACNNNGTC 1 cut(s) 266
RsaI GTAC 2 cut(s) 469, 490
RsaNI GTAC 2 cut(s) 468, 489
RseI CAYNNNNRTG 2 cut(s) 275, 560
SaqAI TTAA 2 cut(s) 422, 894
SatI GCNGC 3 cut(s) 6, 380, 615
Sau3AI GATC 3 cut(s) 41, 124, 867
Sau96I GGNCC 3 cut(s) 500, 533, 734
SchI GAGTC 1 cut(s) 175
SfaNI GCATC 1 cut(s) 844
Sfr274I CTCGAG 1 cut(s) 609
SinI GGWCC 3 cut(s) 500, 533, 734
SlaI CTCGAG 1 cut(s) 609
SmiMI CAYNNNNRTG 2 cut(s) 275, 560
SmlI CTYRAG 1 cut(s) 609
SmoI CTYRAG 1 cut(s) 609
Sse9I AATT 4 cut(s) 12, 76, 283, 789
SsiI CCGC 2 cut(s) 503, 615
SspMI CTAG 3 cut(s) 56, 480, 758
StyI CCWWGG 1 cut(s) 452
TaaI ACNGT 2 cut(s) 226, 899
TaiI ACGT 3 cut(s) 64, 422, 499
TaqI TCGA 4 cut(s) 410, 475, 577, 610
TaqII GACCGA 1 cut(s) 255
TasI AATT 4 cut(s) 12, 76, 283, 789
TauI GCSGC 1 cut(s) 617
TfiI GAWTC 2 cut(s) 191, 457
Tru1I TTAA 2 cut(s) 422, 894
Tru9I TTAA 2 cut(s) 422, 894
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 2 cut(s) 5, 379
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 4 cut(s) 183, 298, 578, 759
TspRI CASTG 1 cut(s) 282
Tth111I GACNNNGTC 1 cut(s) 266
VpaK11BI GGWCC 3 cut(s) 500, 533, 734
XapI RAATTY 1 cut(s) 76
XhoI CTCGAG 1 cut(s) 609
XmnI GAANNNNTTC 1 cut(s) 406
XspI CTAG 3 cut(s) 56, 480, 758
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.