MD05G1352400.v1.1

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002609
Physical Location & Seq
Reverse (-)
46887069 .. 46890878
3810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1352400.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGGCTTTGTTGGTATCCAGAAGGCTTTTGCGTTCATTTGGTTCTGTATTGGCATCAGGCCGCTGTGATTCTTCAAGTAATTTATATCCATCATCATTTCGTGCTACTTTCGATTTTTGCGGCCATGTTCCCGGGGATACCTTACTTTCTGGAGAGTTGTCAGCTGTTTTCAAGCCTGGATCATTTTTTCTAGCAAATCGAAGGCTTTCAACCTCCAACCCGACTCCCGAATCAAGTGGAGGTGCCTTTCCCTCTAATTTATTGTCTGCAAAGCCTGTGGTTGCATCAGAGCGTTCTATAGGGCTCGGTCAGGACCTTGTGATTCCAGTGACAAATTTTCATTATGAAGATAAAGGCTTCACAGTTTTGGCTGGTGATGTTTTCGATGTACCTATTAGGAAGGATATTATTCATCGGGTTGTACTATGGCAGCTAGCAAAACGACAACAGGGAACACATTCAACAAAAACTATTAGTGAGGTTAGTGGGACTGGGAGAAAGCCATGGAATCAGAAGGGTACTGGTCGAGCAAGGCACGGAACATTGCGTGGTCCCCAGTTCAGAGGAGGATGCACAATGCATGGTCCTAAGCCGCGAAGCCATGAAATTAAGCTGAATAAGAAGGTTCGGCGTCTAGGACTAAAGATTGCTCTGACAGCTCGAGCAGCAGAGGGAAAGGCAACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCTTGCTTTGTTCTTCTGGTTTTTGAAGATTTTGAGGTCCCCACGCATAAAACCAAGAATATTGTAAACTACGTCCAGCAAATGGAGGGCTCCAAGAAATTTTTGCTGGTGGATGGTACTAAAACAGAGGATGGTAAACAAATGTTACCCGAAAAGCTCAAGTTGGCTACACAAAATTTGCATTATGTGAACGTGCTGCCCGCAGTAGGTTTGAACGTCTACAGCATCCTGCAGCATGACACACTGGTGATGACCCGAGCAGCTGTAAATGAAATTGTCAAGCGGATGCACACTCCAATCAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

349

Amino Acids

38.13

Weight (kDa)

10.12

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 123 - 340 2.2e-62 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 820
AccI GTMKAC 1 cut(s) 957
AccII CGCG 1 cut(s) 595
AciI CCGC 5 cut(s) 61, 120, 593, 939, 1021
AclWI GGATC 1 cut(s) 187
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 3 cut(s) 334, 836, 913
AcyI GRCGYC 1 cut(s) 631
AfaI GTAC 4 cut(s) 390, 423, 520, 856
AfiI CCNNNNNNNGG 2 cut(s) 820, 944
AgsI TTSAA 6 cut(s) 75, 172, 210, 462, 764, 952
AjnI CCWGG 1 cut(s) 175
AleI CACNNNNGTG 1 cut(s) 983
AluBI AGCT 6 cut(s) 164, 433, 613, 659, 895, 1001
AluI AGCT 6 cut(s) 164, 433, 613, 659, 895, 1001
AlwI GGATC 1 cut(s) 187
Ama87I CYCGRG 3 cut(s) 131, 660, 993
AoxI GGCC 2 cut(s) 58, 121
ApeKI GCWGC 5 cut(s) 430, 665, 934, 970, 998
ApoI RAATTY 3 cut(s) 334, 836, 913
Asp700I GAANNNNTTC 2 cut(s) 205, 457
AspS9I GGNCC 4 cut(s) 313, 551, 584, 775
AsuC2I CCSGG 2 cut(s) 132, 133
AsuHPI GGTGA 2 cut(s) 386, 997
AsuNHI GCTAGC 1 cut(s) 433
AvaI CYCGRG 3 cut(s) 131, 660, 993
AvaII GGWCC 4 cut(s) 313, 551, 584, 775
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 2 cut(s) 306, 830
BbvI GCAGC 5 cut(s) 442, 677, 921, 982, 1010
BccI CCATC 3 cut(s) 97, 845, 863
BciT130I CCWGG 1 cut(s) 177
BciVI GTATCC 2 cut(s) 25, 130
BcnI CCSGG 2 cut(s) 132, 133
BfaI CTAG 3 cut(s) 191, 434, 635
BfmI CTRYAG 3 cut(s) 297, 958, 968
BfuI GTATCC 2 cut(s) 25, 130
BisI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 935, 971, 999
BlsI GCNGC 8 cut(s) 62, 122, 432, 594, 667, 936, 972, 1000
Bme1390I CCNGG 3 cut(s) 132, 133, 177
Bme18I GGWCC 4 cut(s) 313, 551, 584, 775
BmeT110I CYCGRG 3 cut(s) 131, 660, 993
BmgT120I GGNCC 4 cut(s) 313, 551, 584, 775
BmiI GGNNCC 4 cut(s) 244, 553, 777, 829
BmrFI CCNGG 3 cut(s) 132, 133, 177
BmrI ACTGGG 2 cut(s) 501, 550
BmsI GCATC 5 cut(s) 62, 293, 560, 972, 1014
BmtI GCTAGC 1 cut(s) 437
BmuI ACTGGG 2 cut(s) 501, 550
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 881
BpuMI CCSGG 2 cut(s) 132, 133
BsaHI GRCGYC 1 cut(s) 631
BsaJI CCNNGG 3 cut(s) 131, 132, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 820, 944
Bse1I ACTGG 5 cut(s) 326, 496, 526, 556, 987
Bse3DI GCAATG 1 cut(s) 542
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 131, 132, 503
BseGI GGATG 5 cut(s) 575, 856, 874, 963, 1029
BseLI CCNNNNNNNGG 2 cut(s) 820, 944
BseMI GCAATG 1 cut(s) 542
BseNI ACTGG 5 cut(s) 326, 496, 526, 556, 987
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 5 cut(s) 442, 677, 921, 982, 1010
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 2 cut(s) 60, 123
BshNI GGYRCC 1 cut(s) 242
BsiHKCI CYCGRG 3 cut(s) 131, 660, 993
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 3 cut(s) 502, 537, 761
BslI CCNNNNNNNGG 2 cut(s) 820, 944
BsmFI GGGAC 3 cut(s) 502, 537, 761
BsnI GGCC 2 cut(s) 60, 123
BsoBI CYCGRG 3 cut(s) 131, 660, 993
Bsp1286I GDGCHC 2 cut(s) 306, 830
Bsp143I GATC 1 cut(s) 179
Bsp19I CCATGG 1 cut(s) 503
BspACI CCGC 5 cut(s) 61, 120, 593, 939, 1021
BspANI GGCC 2 cut(s) 60, 123
BspFNI CGCG 1 cut(s) 595
BspLI GGNNCC 4 cut(s) 244, 553, 777, 829
BspMAI CTGCAG 1 cut(s) 972
BspOI GCTAGC 1 cut(s) 437
BspPI GGATC 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 542
BsrI ACTGG 5 cut(s) 326, 496, 526, 556, 987
BssECI CCNNGG 3 cut(s) 131, 132, 503
BssMI GATC 1 cut(s) 179
BssNI GRCGYC 1 cut(s) 631
BssT1I CCWWGG 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 2 cut(s) 364, 1043
BstACI GRCGYC 1 cut(s) 631
BstC8I GCNNGC 2 cut(s) 435, 939
BstDEI CTNAG 1 cut(s) 588
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 5 cut(s) 575, 856, 874, 963, 1029
BstFNI CGCG 1 cut(s) 595
BstKTI GATC 1 cut(s) 182
BstMBI GATC 1 cut(s) 179
BstMWI GCNNNNNNNGC 2 cut(s) 656, 665
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 3 cut(s) 130, 131, 175
BstSFI CTRYAG 3 cut(s) 297, 958, 968
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 5 cut(s) 442, 677, 921, 982, 1010
BsuI GTATCC 2 cut(s) 25, 130
BsuRI GGCC 2 cut(s) 60, 123
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 5 cut(s) 575, 856, 874, 963, 1029
BtsIMutI CAGTG 2 cut(s) 333, 980
Cac8I GCNNGC 2 cut(s) 435, 939
Cfr13I GGNCC 4 cut(s) 313, 551, 584, 775
Cfr9I CCCGGG 1 cut(s) 131
CseI GACGC 1 cut(s) 620
Csp6I GTAC 4 cut(s) 389, 422, 519, 855
CspCI CAANNNNNGTGG 2 cut(s) 258, 293
CviAII CATG 5 cut(s) 125, 504, 581, 602, 974
CviQI GTAC 4 cut(s) 389, 422, 519, 855
DdeI CTNAG 1 cut(s) 588
DpnI GATC 1 cut(s) 181
DpnII GATC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 503
Eco24I GRGCYC 2 cut(s) 306, 830
Eco47I GGWCC 4 cut(s) 313, 551, 584, 775
Eco88I CYCGRG 3 cut(s) 131, 660, 993
EcoO109I RGGNCCY 2 cut(s) 313, 775
EcoRII CCWGG 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 503
EcoT22I ATGCAT 1 cut(s) 582
EcoT38I GRGCYC 2 cut(s) 306, 830
ErhI CCWWGG 1 cut(s) 503
FaeI CATG 5 cut(s) 128, 507, 584, 605, 977
FaqI GGGAC 3 cut(s) 502, 537, 761
FatI CATG 5 cut(s) 124, 503, 580, 601, 973
FauI CCCGC 1 cut(s) 946
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 935, 971, 999
FokI GGATG 5 cut(s) 582, 863, 881, 950, 1036
FriOI GRGCYC 2 cut(s) 306, 830
Fsp4HI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 935, 971, 999
FspBI CTAG 3 cut(s) 191, 434, 635
GluI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 935, 971, 999
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 60, 123
HapII CCGG 1 cut(s) 132
HgaI GACGC 1 cut(s) 620
Hin1I GRCGYC 1 cut(s) 631
Hin1II CATG 5 cut(s) 128, 507, 584, 605, 977
HinfI GANTC 5 cut(s) 68, 223, 230, 322, 508
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 386, 997
Hpy166II GTNNAC 4 cut(s) 805, 875, 928, 958
Hpy188I TCNGA 4 cut(s) 289, 513, 563, 654
Hpy188III TCNNGA 4 cut(s) 18, 150, 227, 311
Hpy8I GTNNAC 4 cut(s) 805, 875, 928, 958
HpyAV CCTTC 5 cut(s) 15, 195, 394, 508, 616
HpyCH4III ACNGT 2 cut(s) 364, 1043
HpyCH4IV ACGT 3 cut(s) 810, 930, 954
HpyCH4V TGCA 7 cut(s) 269, 284, 573, 580, 919, 970, 1027
HpyF10VI GCNNNNNNNGC 2 cut(s) 656, 665
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 3 cut(s) 810, 930, 954
Hsp92I GRCGYC 1 cut(s) 631
Hsp92II CATG 5 cut(s) 128, 507, 584, 605, 977
Kzo9I GATC 1 cut(s) 179
LmnI GCTCC 1 cut(s) 833
Lsp1109I GCAGC 5 cut(s) 442, 677, 921, 982, 1010
LweI GCATC 5 cut(s) 62, 293, 560, 972, 1014
MaeI CTAG 3 cut(s) 191, 434, 635
MaeII ACGT 3 cut(s) 810, 930, 954
MaeIII GTNAC 2 cut(s) 328, 882
MalI GATC 1 cut(s) 181
MboI GATC 1 cut(s) 179
MboII GAAGA 4 cut(s) 63, 359, 743, 776
MhlI GDGCHC 2 cut(s) 306, 830
MluCI AATT 7 cut(s) 79, 256, 334, 606, 836, 913, 1011
MlyI GAGTC 1 cut(s) 217
MmeI TCCRAC 1 cut(s) 240
Mph1103I ATGCAT 1 cut(s) 582
MroXI GAANNNNTTC 2 cut(s) 205, 457
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 983
MspA1I CMGCKG 3 cut(s) 63, 164, 1001
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 132, 133, 177
MvaI CCWGG 1 cut(s) 177
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 2 cut(s) 656, 665
NciI CCSGG 2 cut(s) 132, 133
NcoI CCATGG 1 cut(s) 503
NdeII GATC 1 cut(s) 179
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 5 cut(s) 128, 507, 584, 605, 977
NlaIV GGNNCC 4 cut(s) 244, 553, 777, 829
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 582
OliI CACNNNNGTG 1 cut(s) 983
PaeR7I CTCGAG 1 cut(s) 660
PdmI GAANNNNTTC 2 cut(s) 205, 457
PfeI GAWTC 4 cut(s) 68, 230, 322, 508
PflMI CCANNNNNTGG 1 cut(s) 820
PkrI GCNGC 8 cut(s) 62, 122, 432, 594, 667, 936, 972, 1000
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PpuMI RGGWCCY 2 cut(s) 313, 775
Psp5II RGGWCCY 2 cut(s) 313, 775
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 4 cut(s) 244, 553, 777, 829
PspPI GGNCC 4 cut(s) 313, 551, 584, 775
PspPPI RGGWCCY 2 cut(s) 313, 775
PspXI VCTCGAGB 1 cut(s) 660
PstI CTGCAG 1 cut(s) 972
PvuII CAGCTG 2 cut(s) 164, 1001
RsaI GTAC 4 cut(s) 390, 423, 520, 856
RsaNI GTAC 4 cut(s) 389, 422, 519, 855
RseI CAYNNNNRTG 1 cut(s) 983
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 935, 971, 999
Sau3AI GATC 1 cut(s) 179
Sau96I GGNCC 4 cut(s) 313, 551, 584, 775
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 3 cut(s) 132, 133, 177
SduI GDGCHC 2 cut(s) 306, 830
SfaNI GCATC 5 cut(s) 62, 293, 560, 972, 1014
SfcI CTRYAG 3 cut(s) 297, 958, 968
Sfr274I CTCGAG 1 cut(s) 660
SinI GGWCC 4 cut(s) 313, 551, 584, 775
SlaI CTCGAG 1 cut(s) 660
SmaI CCCGGG 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 983
SmlI CTYRAG 2 cut(s) 660, 896
SmoI CTYRAG 2 cut(s) 660, 896
Sse9I AATT 7 cut(s) 79, 256, 334, 606, 836, 913, 1011
SsiI CCGC 5 cut(s) 61, 120, 593, 939, 1021
SspI AATATT 1 cut(s) 799
SspMI CTAG 3 cut(s) 191, 434, 635
StyD4I CCNGG 3 cut(s) 130, 131, 175
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 2 cut(s) 364, 1043
TaiI ACGT 3 cut(s) 813, 933, 957
TaqI TCGA 5 cut(s) 111, 199, 384, 526, 661
TaqII GACCGA 1 cut(s) 296
TasI AATT 7 cut(s) 79, 256, 334, 606, 836, 913, 1011
TatI WGTACW 1 cut(s) 421
TauI GCSGC 3 cut(s) 63, 123, 595
TfiI GAWTC 4 cut(s) 68, 230, 322, 508
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 2 cut(s) 333, 987
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 5 cut(s) 430, 665, 934, 970, 998
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 6 cut(s) 24, 329, 360, 401, 618, 1023
TspGWI ACGGA 1 cut(s) 552
TspMI CCCGGG 1 cut(s) 131
TspRI CASTG 2 cut(s) 333, 987
Van91I CCANNNNNTGG 1 cut(s) 820
VpaK11BI GGWCC 4 cut(s) 313, 551, 584, 775
XapI RAATTY 3 cut(s) 334, 836, 913
XhoI CTCGAG 1 cut(s) 660
XmaI CCCGGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 957
XmnI GAANNNNTTC 2 cut(s) 205, 457
XspI CTAG 3 cut(s) 191, 434, 635
Zsp2I ATGCAT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.