FvH4_3g01410

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002609
Physical Location & Seq
Forward (+)
724820 .. 728575
3756 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01410.t1

Sequence Viewer

Length: 924 bp
ATGGCTTTGTCGGTTTCCAGGAGGCTGCTACGTTCATTTGGTTCTGTTTTGGCATCAGGCAGCTGCGATTCTCCGAGTATATTGTATCCATCTTTACGTGCTTCTCATGGGGTTATCTCTTCCTATGAGGATTCTTCGGCTGTTATAAAGGGTGTGTCATCATCTTTTGCTTATCGAAGGCTTTCAACTCTGACCCCTGAATCAAGTGGAGGTGCATTTCCCTCTGATCTATTGTCCCAAAAGCCTGTGGTAGCATCAGAACATAAAATAGGGAATTATCAAGACCTTGTGATTCCAGTGACAAATTTTCACAATGAAGACAAAGGCTTGATGGTTTTGCCTGGTGATGTTTTTGATGTTCCTATCAGAAAGGATATTGTTCATCGGGTTGTACTATGGCAGCTAGCAAAAAGGCAACAGGGAACACATTCAACAAAAACCATCAGTGAGGTTAGTGGGACCGGTAGAAAGCCATGGAATCAGAAGGGTACTGGTCGAGCGAGGCATGGAACTTTGCGTGGTCCTCAGTTCCGTGGAGGTGCGACAATGCATGGTCCTAAGCCACGAAGTCATGCTATTAAGCTGAATAAGAAGGTTCGGCGTCTTGGGCTAAAAGTTGCTCTGACAGCTCGAGCAGCAGAGGGAAAGCTTCTTGTTTTCGAGGATTTGGAAGTCCCAACACATAAAACCAAGAATATTGTGAACTATGTCAAGCAAATGGAGAACGCCAAGAAAGTTCTAGTAGTGGATGGTGGCCCCATAAGTGATAACCTCAAGCTGGCAACTCAAAACATACATTATGTGAATGTGCTGCCATCAATTGGTTTGAACGTCTACAGCATCCTGCAGCATGACACACTGGTGATGTCTCGCGATGCTGTAAACAAAATTGTTGAACGGATGCATACTCCAATTAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

308

Amino Acids

33.66

Weight (kDa)

10.2

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 113 - 300 8.9e-71 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 821
AccI GTMKAC 1 cut(s) 834
AccII CGCG 1 cut(s) 873
AcsI RAATTY 1 cut(s) 304
AcyI GRCGYC 1 cut(s) 601
AfaI GTAC 2 cut(s) 393, 490
AfiI CCNNNNNNNGG 2 cut(s) 778, 821
AgeI ACCGGT 1 cut(s) 461
AgsI TTSAA 4 cut(s) 186, 432, 829, 896
AjnI CCWGG 2 cut(s) 17, 340
AleI CACNNNNGTG 1 cut(s) 860
AluBI AGCT 6 cut(s) 63, 403, 583, 629, 649, 778
AluI AGCT 6 cut(s) 63, 403, 583, 629, 649, 778
Alw26I GTCTC 1 cut(s) 873
Ama87I CYCGRG 1 cut(s) 630
AoxI GGCC 1 cut(s) 754
ApeKI GCWGC 7 cut(s) 25, 60, 63, 400, 635, 811, 847
ApoI RAATTY 1 cut(s) 304
AsiGI ACCGGT 1 cut(s) 461
Asp700I GAANNNNTTC 2 cut(s) 181, 427
AspS9I GGNCC 4 cut(s) 459, 521, 554, 755
AsuHPI GGTGA 2 cut(s) 356, 874
AsuNHI GCTAGC 1 cut(s) 403
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 3 cut(s) 459, 521, 554
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 7 cut(s) 12, 50, 72, 412, 647, 798, 859
BccI CCATC 5 cut(s) 97, 325, 449, 743, 823
BciT130I CCWGG 2 cut(s) 19, 342
BciVI GTATCC 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 873
BfaI CTAG 2 cut(s) 404, 740
BfmI CTRYAG 2 cut(s) 835, 845
BfuI GTATCC 1 cut(s) 96
BisI GCNGC 7 cut(s) 26, 61, 64, 401, 636, 812, 848
BlsI GCNGC 7 cut(s) 27, 62, 65, 402, 637, 813, 849
Bme1390I CCNGG 2 cut(s) 19, 342
Bme18I GGWCC 3 cut(s) 459, 521, 554
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 4 cut(s) 459, 521, 554, 755
BmiI GGNNCC 2 cut(s) 460, 757
BmrFI CCNGG 2 cut(s) 19, 342
BmsI GCATC 5 cut(s) 62, 263, 849, 865, 891
BmtI GCTAGC 1 cut(s) 407
BpiI GAAGAC 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 758
BsaAI YACGTR 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 601
BsaJI CCNNGG 2 cut(s) 473, 532
BsaWI WCCGGW 1 cut(s) 461
Bsc4I CCNNNNNNNGG 2 cut(s) 778, 821
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 3 cut(s) 296, 496, 864
BseBI CCWGG 2 cut(s) 19, 342
BseDI CCNNGG 2 cut(s) 473, 532
BseGI GGATG 3 cut(s) 754, 840, 906
BseLI CCNNNNNNNGG 2 cut(s) 778, 821
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 3 cut(s) 296, 496, 864
BseXI GCAGC 7 cut(s) 12, 50, 72, 412, 647, 798, 859
Bsh1236I CGCG 1 cut(s) 873
BshFI GGCC 1 cut(s) 756
BshTI ACCGGT 1 cut(s) 461
BsiHKCI CYCGRG 1 cut(s) 630
BsiSI CCGG 1 cut(s) 462
BslFI GGGAC 3 cut(s) 220, 472, 659
BslI CCNNNNNNNGG 2 cut(s) 778, 821
BsmAI GTCTC 1 cut(s) 873
BsmFI GGGAC 3 cut(s) 220, 472, 659
BsnI GGCC 1 cut(s) 756
BsoBI CYCGRG 1 cut(s) 630
Bsp143I GATC 1 cut(s) 226
Bsp19I CCATGG 1 cut(s) 473
Bsp68I TCGCGA 1 cut(s) 873
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 1 cut(s) 538
BspFNI CGCG 1 cut(s) 873
BspLI GGNNCC 2 cut(s) 460, 757
BspMAI CTGCAG 1 cut(s) 849
BspOI GCTAGC 1 cut(s) 407
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 3 cut(s) 296, 496, 864
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 2 cut(s) 473, 532
BssMI GATC 1 cut(s) 226
BssNI GRCGYC 1 cut(s) 601
BssT1I CCWWGG 1 cut(s) 473
Bst2UI CCWGG 2 cut(s) 19, 342
Bst4CI ACNGT 1 cut(s) 920
Bst6I CTCTTC 1 cut(s) 124
BstACI GRCGYC 1 cut(s) 601
BstBAI YACGTR 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 405, 780
BstDEI CTNAG 2 cut(s) 525, 558
BstDSI CCRYGG 2 cut(s) 473, 532
BstF5I GGATG 3 cut(s) 754, 840, 906
BstFNI CGCG 1 cut(s) 873
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 873
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 3 cut(s) 607, 626, 635
BstNI CCWGG 2 cut(s) 19, 342
BstSCI CCNGG 2 cut(s) 17, 340
BstSFI CTRYAG 2 cut(s) 835, 845
BstUI CGCG 1 cut(s) 873
BstV1I GCAGC 7 cut(s) 12, 50, 72, 412, 647, 798, 859
BstV2I GAAGAC 1 cut(s) 324
BsuI GTATCC 1 cut(s) 96
BsuRI GGCC 1 cut(s) 756
BtgI CCRYGG 2 cut(s) 473, 532
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 3 cut(s) 754, 840, 906
BtsIMutI CAGTG 3 cut(s) 303, 451, 857
BtuMI TCGCGA 1 cut(s) 873
Cac8I GCNNGC 2 cut(s) 405, 780
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 4 cut(s) 459, 521, 554, 755
CseI GACGC 1 cut(s) 590
Csp6I GTAC 2 cut(s) 392, 489
CspAI ACCGGT 1 cut(s) 461
CviAII CATG 6 cut(s) 107, 474, 506, 551, 572, 851
CviQI GTAC 2 cut(s) 392, 489
DdeI CTNAG 2 cut(s) 525, 558
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 473
Eco47I GGWCC 3 cut(s) 459, 521, 554
Eco88I CYCGRG 1 cut(s) 630
EcoRII CCWGG 2 cut(s) 17, 340
EcoT14I CCWWGG 1 cut(s) 473
EcoT22I ATGCAT 2 cut(s) 552, 906
ErhI CCWWGG 1 cut(s) 473
FaeI CATG 6 cut(s) 110, 477, 509, 554, 575, 854
FaqI GGGAC 3 cut(s) 220, 472, 659
FatI CATG 6 cut(s) 106, 473, 505, 550, 571, 850
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 7 cut(s) 26, 61, 64, 401, 636, 812, 848
FokI GGATG 3 cut(s) 761, 827, 913
Fsp4HI GCNGC 7 cut(s) 26, 61, 64, 401, 636, 812, 848
FspBI CTAG 2 cut(s) 404, 740
GluI GCNGC 7 cut(s) 26, 61, 64, 401, 636, 812, 848
HaeIII GGCC 1 cut(s) 756
HapII CCGG 1 cut(s) 462
HgaI GACGC 1 cut(s) 590
Hin1I GRCGYC 1 cut(s) 601
Hin1II CATG 6 cut(s) 110, 477, 509, 554, 575, 854
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 5 cut(s) 68, 131, 200, 292, 478
HpaII CCGG 1 cut(s) 462
HphI GGTGA 2 cut(s) 356, 874
Hpy166II GTNNAC 3 cut(s) 703, 835, 883
Hpy188I TCNGA 7 cut(s) 75, 192, 226, 259, 368, 483, 624
Hpy188III TCNNGA 2 cut(s) 281, 872
Hpy8I GTNNAC 3 cut(s) 703, 835, 883
HpyAV CCTTC 3 cut(s) 171, 478, 586
HpyCH4III ACNGT 1 cut(s) 920
HpyCH4IV ACGT 3 cut(s) 31, 97, 831
HpyCH4V TGCA 4 cut(s) 215, 550, 847, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 607, 626, 635
HpyF3I CTNAG 2 cut(s) 525, 558
HpySE526I ACGT 3 cut(s) 31, 97, 831
Hsp92I GRCGYC 1 cut(s) 601
Hsp92II CATG 6 cut(s) 110, 477, 509, 554, 575, 854
Kzo9I GATC 1 cut(s) 226
Lsp1109I GCAGC 7 cut(s) 12, 50, 72, 412, 647, 798, 859
LweI GCATC 5 cut(s) 62, 263, 849, 865, 891
MaeI CTAG 2 cut(s) 404, 740
MaeII ACGT 3 cut(s) 31, 97, 831
MaeIII GTNAC 1 cut(s) 298
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 3 cut(s) 111, 126, 329
MfeI CAATTG 1 cut(s) 819
MluCI AATT 5 cut(s) 274, 304, 819, 888, 912
Mph1103I ATGCAT 2 cut(s) 552, 906
MroXI GAANNNNTTC 2 cut(s) 181, 427
MseI TTAA 2 cut(s) 579, 915
MslI CAYNNNNRTG 1 cut(s) 860
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 2 cut(s) 19, 342
MunI CAATTG 1 cut(s) 819
MvaI CCWGG 2 cut(s) 19, 342
MvnI CGCG 1 cut(s) 873
MwoI GCNNNNNNNGC 3 cut(s) 607, 626, 635
NcoI CCATGG 1 cut(s) 473
NdeII GATC 1 cut(s) 226
NheI GCTAGC 1 cut(s) 403
NlaIII CATG 6 cut(s) 110, 477, 509, 554, 575, 854
NlaIV GGNNCC 2 cut(s) 460, 757
NmuCI GTSAC 1 cut(s) 298
NruI TCGCGA 1 cut(s) 873
NsiI ATGCAT 2 cut(s) 552, 906
OliI CACNNNNGTG 1 cut(s) 860
PaeR7I CTCGAG 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 181, 427
PfeI GAWTC 5 cut(s) 68, 131, 200, 292, 478
PflMI CCANNNNNTGG 1 cut(s) 821
PfoI TCCNGGA 1 cut(s) 17
PinAI ACCGGT 1 cut(s) 461
PkrI GCNGC 7 cut(s) 27, 62, 65, 402, 637, 813, 849
Ppu21I YACGTR 1 cut(s) 98
PsiI TTATAA 1 cut(s) 146
Psp6I CCWGG 2 cut(s) 17, 340
PspGI CCWGG 2 cut(s) 17, 340
PspN4I GGNNCC 2 cut(s) 460, 757
PspPI GGNCC 4 cut(s) 459, 521, 554, 755
PspXI VCTCGAGB 1 cut(s) 630
PstI CTGCAG 1 cut(s) 849
PvuII CAGCTG 1 cut(s) 63
RruI TCGCGA 1 cut(s) 873
RsaI GTAC 2 cut(s) 393, 490
RsaNI GTAC 2 cut(s) 392, 489
RseI CAYNNNNRTG 1 cut(s) 860
SaqAI TTAA 2 cut(s) 579, 915
SatI GCNGC 7 cut(s) 26, 61, 64, 401, 636, 812, 848
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 4 cut(s) 459, 521, 554, 755
ScrFI CCNGG 2 cut(s) 19, 342
SfaNI GCATC 5 cut(s) 62, 263, 849, 865, 891
SfcI CTRYAG 2 cut(s) 835, 845
Sfr274I CTCGAG 1 cut(s) 630
SinI GGWCC 3 cut(s) 459, 521, 554
SlaI CTCGAG 1 cut(s) 630
SmiMI CAYNNNNRTG 1 cut(s) 860
SmlI CTYRAG 2 cut(s) 630, 773
SmoI CTYRAG 2 cut(s) 630, 773
Sse9I AATT 5 cut(s) 274, 304, 819, 888, 912
SspI AATATT 1 cut(s) 697
SspMI CTAG 2 cut(s) 404, 740
StyD4I CCNGG 2 cut(s) 17, 340
StyI CCWWGG 1 cut(s) 473
TaaI ACNGT 1 cut(s) 920
TaiI ACGT 3 cut(s) 34, 100, 834
TaqI TCGA 4 cut(s) 175, 496, 631, 660
TasI AATT 5 cut(s) 274, 304, 819, 888, 912
TatI WGTACW 1 cut(s) 391
TfiI GAWTC 5 cut(s) 68, 131, 200, 292, 478
Tru1I TTAA 2 cut(s) 579, 915
Tru9I TTAA 2 cut(s) 579, 915
TscAI CASTG 3 cut(s) 303, 451, 864
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 7 cut(s) 25, 60, 63, 400, 635, 811, 847
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 3 cut(s) 24, 330, 371
TspGWI ACGGA 2 cut(s) 521, 913
TspRI CASTG 3 cut(s) 303, 451, 864
Van91I CCANNNNNTGG 1 cut(s) 821
VpaK11BI GGWCC 3 cut(s) 459, 521, 554
XapI RAATTY 1 cut(s) 304
XhoI CTCGAG 1 cut(s) 630
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 2 cut(s) 181, 427
XspI CTAG 2 cut(s) 404, 740
Zsp2I ATGCAT 2 cut(s) 552, 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.