FvH4_3g43720

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002609
Physical Location & Seq
Reverse (-)
36403146 .. 36406784
3639 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43720.t1

Sequence Viewer

Length: 819 bp
ATGCTGAATCTGTTTTGGAGTATGGCTTTGTTCATTTCCAGAAGAATCGCGCATTCATTTGGTTCTGGTTTAGCATCTGCCCATGTTACCATGGGTGGATCATTGATTTGTGCTTGTCGAATGCTTTCGACTTCCGTCTTGACCCAAGAGTCATTTTGCTCTGACTCATTGTCCTTAAAGCCTGAGGGGGCTTCAGAGTGCACTACAGTGACAAAATTTGGTGATGAAAAGAAAGGATCCACAACTTTGGCTGATGATATTTTTGATGCACCAATTACAATGGATATTGCTCGTCAGATCTTCTTCTTCTTTCGTTGGATTCATCCGGTTAAAATGTGGCAGCTAGCAAAAAGGCAACAGCCATCGAATTGGAAGGATAGTGGTCGAACCAGCCATGGAAACTTGAGTGATCCCCAGCATAGAGGTGTCAACAGACGAAGTCATACTTCTAAGAATGTAGAACTTCTGGGACTAAAGATGGCTCTGTCATCTCGAGCGGCACAGGGAAAGCTTCTGGTTTTTGAGGATTTTGGGATCTACAGAAATAAACCCGATAACGTTGTGAACTATGTCAAAGAAAGAATGAAAAAGGAGAAGAAGAAAAAAATTCTGCTGGTGGATGGTGGTCCAATAAGTAAAGAGCTCAAGTTGGCTACTGAACGTTTACGTTGTGTGACTGTGATGCCCTCAAGTGAGTTGGATGCCCTTAGCATGATCTCTTATGACAGAGTGGTGATGTCTCGTGATGCTGTAAAAAAAATTACTGAGAAAATTATGCATACACCGATCAACCATGGAAGCAACACAACATACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

273

Amino Acids

30.64

Weight (kDa)

9.68

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 127 - 256 1.6e-15 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 148
AccBSI CCGCTC 1 cut(s) 497
AccII CGCG 1 cut(s) 50
AciI CCGC 1 cut(s) 497
AclI AACGTT 2 cut(s) 558, 661
AclWI GGATC 5 cut(s) 106, 231, 244, 404, 542
AcsI RAATTY 2 cut(s) 215, 606
AcuI CTGAAG 1 cut(s) 177
AhdI GACNNNNNGTC 1 cut(s) 169
AleI CACNNNNGTG 1 cut(s) 206
AluBI AGCT 3 cut(s) 343, 511, 643
AluI AGCT 3 cut(s) 343, 511, 643
Alw21I GWGCWC 2 cut(s) 203, 645
Alw26I GTCTC 1 cut(s) 744
Alw44I GTGCAC 1 cut(s) 199
AlwI GGATC 5 cut(s) 106, 231, 244, 404, 542
Ama87I CYCGRG 1 cut(s) 492
ApaLI GTGCAC 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 340
ApoI RAATTY 2 cut(s) 215, 606
Asp700I GAANNNNTTC 1 cut(s) 124
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 626
AsuHPI GGTGA 2 cut(s) 233, 745
AsuNHI GCTAGC 1 cut(s) 343
AvaI CYCGRG 1 cut(s) 492
AvaII GGWCC 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 203
BamHI GGATCC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 645
BauI CACGAG 1 cut(s) 741
Bbv12I GWGCWC 2 cut(s) 203, 645
BbvI GCAGC 1 cut(s) 352
BccI CCATC 3 cut(s) 370, 472, 614
BcoDI GTCTC 1 cut(s) 744
BfaI CTAG 1 cut(s) 344
BfmI CTRYAG 2 cut(s) 204, 538
BglII AGATCT 1 cut(s) 297
BisI GCNGC 2 cut(s) 341, 498
BlsI GCNGC 2 cut(s) 342, 499
Bme18I GGWCC 1 cut(s) 626
BmeRI GACNNNNNGTC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 492
BmgT120I GGNCC 1 cut(s) 626
BmiI GGNNCC 1 cut(s) 238
BmsI GCATC 5 cut(s) 83, 256, 672, 691, 736
BmtI GCTAGC 1 cut(s) 347
BoxI GACNNNNGTC 1 cut(s) 134
Bpu10I CCTNAGC 1 cut(s) 707
BpuEI CTTGAG 3 cut(s) 424, 629, 673
BsaJI CCNNGG 3 cut(s) 90, 394, 793
BsaWI WCCGGW 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 818
Bse21I CCTNAGG 1 cut(s) 183
BseDI CCNNGG 3 cut(s) 90, 394, 793
BseGI GGATG 3 cut(s) 322, 625, 706
BseMII CTCAG 2 cut(s) 174, 756
BseNI ACTGG 1 cut(s) 818
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 1 cut(s) 352
BseYI CCCAGC 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 203, 645
BsiHKCI CYCGRG 1 cut(s) 492
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 483
BsmAI GTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 483
BsmI GAATGC 2 cut(s) 52, 126
BsoBI CYCGRG 1 cut(s) 492
Bsp1286I GDGCHC 2 cut(s) 203, 645
Bsp143I GATC 7 cut(s) 98, 236, 297, 409, 534, 714, 786
Bsp19I CCATGG 3 cut(s) 90, 394, 793
BspACI CCGC 1 cut(s) 497
BspCNI CTCAG 2 cut(s) 175, 757
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 1 cut(s) 238
BspOI GCTAGC 1 cut(s) 347
BspPI GGATC 5 cut(s) 106, 231, 244, 404, 542
BsrBI CCGCTC 1 cut(s) 497
BsrI ACTGG 1 cut(s) 818
BssECI CCNNGG 3 cut(s) 90, 394, 793
BssMI GATC 7 cut(s) 98, 236, 297, 409, 534, 714, 786
BssSI CACGAG 1 cut(s) 741
BssT1I CCWWGG 3 cut(s) 90, 394, 793
Bst2BI CACGAG 1 cut(s) 741
Bst4CI ACNGT 2 cut(s) 208, 679
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 4 cut(s) 183, 450, 707, 765
BstDSI CCRYGG 3 cut(s) 90, 394, 793
BstF5I GGATG 3 cut(s) 322, 625, 706
BstFNI CGCG 1 cut(s) 50
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 7 cut(s) 101, 239, 300, 412, 537, 717, 789
BstMAI GTCTC 1 cut(s) 744
BstMBI GATC 7 cut(s) 98, 236, 297, 409, 534, 714, 786
BstPAI GACNNNNGTC 1 cut(s) 134
BstSFI CTRYAG 2 cut(s) 204, 538
BstSLI GKGCMC 1 cut(s) 203
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 1 cut(s) 352
BstX2I RGATCY 3 cut(s) 236, 297, 534
BstXI CCANNNNNNTGG 2 cut(s) 247, 369
BstYI RGATCY 3 cut(s) 236, 297, 534
Bsu36I CCTNAGG 1 cut(s) 183
BtgI CCRYGG 3 cut(s) 90, 394, 793
BtsCI GGATG 3 cut(s) 322, 625, 706
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 345
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 626
CviAII CATG 5 cut(s) 83, 91, 395, 712, 794
DdeI CTNAG 4 cut(s) 183, 450, 707, 765
DpnI GATC 7 cut(s) 100, 238, 299, 411, 536, 716, 788
DpnII GATC 7 cut(s) 98, 236, 297, 409, 534, 714, 786
DrdI GACNNNNNNGTC 1 cut(s) 148
DriI GACNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 148
Eam1105I GACNNNNNGTC 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 643
Eco130I CCWWGG 3 cut(s) 90, 394, 793
Eco24I GRGCYC 1 cut(s) 645
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 643
Eco57I CTGAAG 1 cut(s) 177
Eco81I CCTNAGG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 492
EcoICRI GAGCTC 1 cut(s) 643
EcoT14I CCWWGG 3 cut(s) 90, 394, 793
EcoT22I ATGCAT 1 cut(s) 780
EcoT38I GRGCYC 1 cut(s) 645
ErhI CCWWGG 3 cut(s) 90, 394, 793
FaeI CATG 5 cut(s) 86, 94, 398, 715, 797
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FaqI GGGAC 1 cut(s) 483
FatI CATG 5 cut(s) 82, 90, 394, 711, 793
Fnu4HI GCNGC 2 cut(s) 341, 498
FokI GGATG 3 cut(s) 309, 632, 713
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 2 cut(s) 341, 498
FspBI CTAG 1 cut(s) 344
GlaI GCGC 1 cut(s) 51
GluI GCNGC 2 cut(s) 341, 498
GsaI CCCAGC 1 cut(s) 418
HapII CCGG 1 cut(s) 326
HhaI GCGC 1 cut(s) 52
Hin1II CATG 5 cut(s) 86, 94, 398, 715, 797
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 5 cut(s) 7, 45, 149, 164, 319
HpaII CCGG 1 cut(s) 326
HphI GGTGA 2 cut(s) 233, 745
Hpy166II GTNNAC 4 cut(s) 201, 430, 565, 665
Hpy188I TCNGA 3 cut(s) 163, 196, 297
Hpy188III TCNNGA 4 cut(s) 39, 139, 492, 743
Hpy8I GTNNAC 4 cut(s) 201, 430, 565, 665
HpyAV CCTTC 1 cut(s) 367
HpyCH4III ACNGT 2 cut(s) 208, 679
HpyCH4IV ACGT 3 cut(s) 558, 661, 667
HpyCH4V TGCA 3 cut(s) 201, 269, 778
HpyF3I CTNAG 4 cut(s) 183, 450, 707, 765
HpySE526I ACGT 3 cut(s) 558, 661, 667
Hsp92II CATG 5 cut(s) 86, 94, 398, 715, 797
HspAI GCGC 1 cut(s) 50
Kzo9I GATC 7 cut(s) 98, 236, 297, 409, 534, 714, 786
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 5 cut(s) 83, 256, 672, 691, 736
MaeI CTAG 1 cut(s) 344
MaeII ACGT 3 cut(s) 558, 661, 667
MaeIII GTNAC 3 cut(s) 85, 208, 673
MalI GATC 7 cut(s) 100, 238, 299, 411, 536, 716, 788
MbiI CCGCTC 1 cut(s) 497
MboI GATC 7 cut(s) 98, 236, 297, 409, 534, 714, 786
MboII GAAGA 6 cut(s) 54, 292, 295, 298, 607, 610
MflI RGATCY 3 cut(s) 236, 297, 534
MhlI GDGCHC 2 cut(s) 203, 645
MluCI AATT 6 cut(s) 215, 273, 367, 606, 759, 771
MlyI GAGTC 2 cut(s) 158, 158
MmeI TCCRAC 2 cut(s) 296, 678
MnlI CCTC 4 cut(s) 178, 416, 517, 697
Mph1103I ATGCAT 1 cut(s) 780
MroXI GAANNNNTTC 1 cut(s) 124
MseI TTAA 2 cut(s) 176, 330
MslI CAYNNNNRTG 3 cut(s) 206, 423, 814
MspI CCGG 1 cut(s) 326
Mva1269I GAATGC 2 cut(s) 52, 126
MvnI CGCG 1 cut(s) 50
NcoI CCATGG 3 cut(s) 90, 394, 793
NdeII GATC 7 cut(s) 98, 236, 297, 409, 534, 714, 786
NheI GCTAGC 1 cut(s) 343
NlaIII CATG 5 cut(s) 86, 94, 398, 715, 797
NlaIV GGNNCC 1 cut(s) 238
NmuCI GTSAC 2 cut(s) 208, 673
NsiI ATGCAT 1 cut(s) 780
OliI CACNNNNGTG 1 cut(s) 206
PaeR7I CTCGAG 1 cut(s) 492
PctI GAATGC 2 cut(s) 52, 126
PdmI GAANNNNTTC 1 cut(s) 124
PfeI GAWTC 3 cut(s) 7, 45, 319
PflFI GACNNNGTC 1 cut(s) 438
PkrI GCNGC 2 cut(s) 342, 499
PleI GAGTC 2 cut(s) 157, 158
PpsI GAGTC 2 cut(s) 157, 158
PshAI GACNNNNGTC 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 645
Psp1406I AACGTT 2 cut(s) 558, 661
PspFI CCCAGC 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 1 cut(s) 626
PsuI RGATCY 3 cut(s) 236, 297, 534
PsyI GACNNNGTC 1 cut(s) 438
RseI CAYNNNNRTG 3 cut(s) 206, 423, 814
SacI GAGCTC 1 cut(s) 645
SaqAI TTAA 2 cut(s) 176, 330
SatI GCNGC 2 cut(s) 341, 498
Sau3AI GATC 7 cut(s) 98, 236, 297, 409, 534, 714, 786
Sau96I GGNCC 1 cut(s) 626
SchI GAGTC 2 cut(s) 158, 158
SduI GDGCHC 2 cut(s) 203, 645
SetI ASST 7 cut(s) 345, 427, 513, 561, 645, 664, 670
SfaNI GCATC 5 cut(s) 83, 256, 672, 691, 736
SfcI CTRYAG 2 cut(s) 204, 538
Sfr274I CTCGAG 1 cut(s) 492
SinI GGWCC 1 cut(s) 626
SlaI CTCGAG 1 cut(s) 492
SmiMI CAYNNNNRTG 3 cut(s) 206, 423, 814
SmlI CTYRAG 4 cut(s) 403, 492, 644, 688
SmoI CTYRAG 4 cut(s) 403, 492, 644, 688
Sse9I AATT 6 cut(s) 215, 273, 367, 606, 759, 771
SsiI CCGC 1 cut(s) 497
SspMI CTAG 1 cut(s) 344
SstI GAGCTC 1 cut(s) 645
StyI CCWWGG 3 cut(s) 90, 394, 793
TaaI ACNGT 2 cut(s) 208, 679
TaiI ACGT 3 cut(s) 561, 664, 670
TaqI TCGA 5 cut(s) 118, 128, 365, 385, 493
TasI AATT 6 cut(s) 215, 273, 367, 606, 759, 771
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 3 cut(s) 7, 45, 319
Tru1I TTAA 2 cut(s) 176, 330
Tru9I TTAA 2 cut(s) 176, 330
TscAI CASTG 1 cut(s) 213
TseFI GTSAC 2 cut(s) 208, 673
TseI GCWGC 1 cut(s) 340
Tsp45I GTSAC 2 cut(s) 208, 673
TspDTI ATGAA 5 cut(s) 22, 45, 240, 311, 599
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 1 cut(s) 213
Tth111I GACNNNGTC 1 cut(s) 438
VneI GTGCAC 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 626
XapI RAATTY 2 cut(s) 215, 606
XhoI CTCGAG 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 124
XspI CTAG 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.