Rw5G047770

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002609
Physical Location & Seq
Reverse (-)
82758477 .. 82760577
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047770.1

Sequence Viewer

Length: 858 bp
ATGGCTTTGTTCATTTCCAGAAGAATCGTACGTTCATTTGGTTCTGGTTTAGCATCAGGCCATGTTTCCAAGGGTGGATCAGTGTTTCATGCTTGTCAAATACTTTCGACATTAGTCTTGACCCATGAGTCGAGTGGAGGTGCATTTTGCTCTGATTCATTGTCCTTAAAGCCGCCCGGGGCTTCGGAGTGCACTACAGGACTTTGTCTTGATCTTGTAATTTCAGTGACAAAATTTCTTGATGAAAAGAAAGGCTCCACAACTTTGGCTAGTGATGTTTTTGATGCACCTATTACGATGGATATTGCTCGTCGGATATTTTTCTTCTTTCGTTGGCTTCATCCAGTTGTGCTGTGGCAGCTAGCAAAAAGGCAACAGTGTTTTGGAATTCTTGTAAAGCCATCGAATTGGAAGGAGAGTGGTCGAGCCAGCCATGGAACTTTGAGTGATCCCCAGCTCAGAGGTCTTAAGCCACGAAGTCATACTATTAAGAATGTCGAACGTCTGGGATTAAAGATTGCTCTGACATCTCGAGCGGCACAGGGAAAGCTTCTGGTTTTTGAGGATTTTGGGATCTACAGAAATAAACCCAAGAACATTGTGAACTATGTCAAGCAAAGACGGAAAAAGGAGAAAAAGAAGAAAATTCTGCTGGTGGATGGTGGTCCAATAAGTGAAAAGCTCAAGTTGGCTACTAAAAATTTACATTACGTGACTGTGCTGCCTTCAAGTGAGTTGGACGTCGACAGCATCCTTTACTATGACACACTGGTGATGTCCCGTGATGCTGTAAATAAAATTGCTGAGCAAATTATGTATACTCCAATCAACCATGGATCCAGCACGACATACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

285

Amino Acids

31.73

Weight (kDa)

9.81

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 89 - 269 2.4e-21 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
AatII GACGTC 1 cut(s) 744
AccBSI CCGCTC 1 cut(s) 536
AccI GTMKAC 2 cut(s) 744, 818
AciI CCGC 2 cut(s) 173, 536
AclWI GGATC 5 cut(s) 85, 443, 581, 831, 844
AcsI RAATTY 4 cut(s) 233, 387, 645, 700
AcyI GRCGYC 1 cut(s) 741
AfaI GTAC 1 cut(s) 30
AflII CTTAAG 1 cut(s) 467
AgsI TTSAA 1 cut(s) 729
AleI CACNNNNGTG 1 cut(s) 770
AluBI AGCT 4 cut(s) 361, 457, 550, 682
AluI AGCT 4 cut(s) 361, 457, 550, 682
Alw21I GWGCWC 1 cut(s) 194
Alw44I GTGCAC 1 cut(s) 190
AlwI GGATC 5 cut(s) 85, 443, 581, 831, 844
Ama87I CYCGRG 2 cut(s) 176, 531
AoxI GGCC 1 cut(s) 58
ApaLI GTGCAC 1 cut(s) 190
ApeKI GCWGC 2 cut(s) 358, 721
ApoI RAATTY 4 cut(s) 233, 387, 645, 700
AspS9I GGNCC 1 cut(s) 665
AsuC2I CCSGG 2 cut(s) 177, 178
AsuHPI GGTGA 1 cut(s) 784
AsuNHI GCTAGC 1 cut(s) 361
AvaI CYCGRG 2 cut(s) 176, 531
AvaII GGWCC 1 cut(s) 665
BaeGI GKGCMC 1 cut(s) 194
BamHI GGATCC 1 cut(s) 836
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 370, 708
BccI CCATC 3 cut(s) 292, 409, 653
BcnI CCSGG 2 cut(s) 177, 178
BfaI CTAG 2 cut(s) 270, 362
BfmI CTRYAG 2 cut(s) 195, 577
BfrI CTTAAG 1 cut(s) 467
BisI GCNGC 4 cut(s) 173, 359, 537, 722
BlpI GCTNAGC 1 cut(s) 804
BlsI GCNGC 4 cut(s) 174, 360, 538, 723
Bme1390I CCNGG 2 cut(s) 177, 178
Bme18I GGWCC 1 cut(s) 665
BmeT110I CYCGRG 2 cut(s) 176, 531
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 2 cut(s) 256, 838
BmrFI CCNGG 2 cut(s) 177, 178
BmsI GCATC 4 cut(s) 62, 274, 759, 775
BmtI GCTAGC 1 cut(s) 365
BoxI GACNNNNGTC 1 cut(s) 113
Bpu1102I GCTNAGC 1 cut(s) 804
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 2 cut(s) 177, 178
BsaAI YACGTR 1 cut(s) 712
BsaHI GRCGYC 1 cut(s) 741
BsaJI CCNNGG 5 cut(s) 69, 176, 177, 433, 832
Bse1I ACTGG 3 cut(s) 344, 774, 857
BseDI CCNNGG 5 cut(s) 69, 176, 177, 433, 832
BseGI GGATG 3 cut(s) 340, 664, 750
BseMII CTCAG 2 cut(s) 472, 795
BseNI ACTGG 3 cut(s) 344, 774, 857
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 2 cut(s) 370, 708
BseYI CCCAGC 1 cut(s) 453
BshFI GGCC 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 194
BsiHKCI CYCGRG 2 cut(s) 176, 531
BsiSI CCGG 1 cut(s) 177
BsiWI CGTACG 1 cut(s) 28
BslFI GGGAC 1 cut(s) 763
BsmFI GGGAC 1 cut(s) 763
BsnI GGCC 1 cut(s) 60
BsoBI CYCGRG 2 cut(s) 176, 531
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 5 cut(s) 77, 211, 448, 573, 836
Bsp1720I GCTNAGC 1 cut(s) 804
Bsp19I CCATGG 2 cut(s) 433, 832
BspACI CCGC 2 cut(s) 173, 536
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 2 cut(s) 471, 796
BspLI GGNNCC 2 cut(s) 256, 838
BspOI GCTAGC 1 cut(s) 365
BspPI GGATC 5 cut(s) 85, 443, 581, 831, 844
BspTI CTTAAG 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 536
BsrI ACTGG 3 cut(s) 344, 774, 857
BssECI CCNNGG 5 cut(s) 69, 176, 177, 433, 832
BssMI GATC 5 cut(s) 77, 211, 448, 573, 836
BssNAI GTATAC 1 cut(s) 819
BssNI GRCGYC 1 cut(s) 741
BssT1I CCWWGG 3 cut(s) 69, 433, 832
Bst1107I GTATAC 1 cut(s) 819
Bst4CI ACNGT 2 cut(s) 378, 718
BstACI GRCGYC 1 cut(s) 741
BstAFI CTTAAG 1 cut(s) 467
BstBAI YACGTR 1 cut(s) 712
BstC8I GCNNGC 2 cut(s) 363, 430
BstDEI CTNAG 2 cut(s) 458, 804
BstDSI CCRYGG 2 cut(s) 433, 832
BstF5I GGATG 3 cut(s) 340, 664, 750
BstKTI GATC 5 cut(s) 80, 214, 451, 576, 839
BstMBI GATC 5 cut(s) 77, 211, 448, 573, 836
BstMWI GCNNNNNNNGC 1 cut(s) 358
BstPAI GACNNNNGTC 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 175, 176
BstSFI CTRYAG 2 cut(s) 195, 577
BstSLI GKGCMC 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 370, 708
BstX2I RGATCY 2 cut(s) 573, 836
BstXI CCANNNNNNTGG 2 cut(s) 265, 408
BstYI RGATCY 2 cut(s) 573, 836
BstZ17I GTATAC 1 cut(s) 819
BsuRI GGCC 1 cut(s) 60
BtgI CCRYGG 2 cut(s) 433, 832
BtsCI GGATG 3 cut(s) 340, 664, 750
BtsIMutI CAGTG 4 cut(s) 87, 231, 383, 767
Cac8I GCNNGC 2 cut(s) 363, 430
Cfr13I GGNCC 1 cut(s) 665
Cfr9I CCCGGG 1 cut(s) 176
Csp6I GTAC 1 cut(s) 29
CviAII CATG 5 cut(s) 62, 89, 125, 434, 833
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 2 cut(s) 458, 804
DpnI GATC 5 cut(s) 79, 213, 450, 575, 838
DpnII GATC 5 cut(s) 77, 211, 448, 573, 836
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
Eco130I CCWWGG 3 cut(s) 69, 433, 832
Eco47I GGWCC 1 cut(s) 665
Eco88I CYCGRG 2 cut(s) 176, 531
EcoRI GAATTC 1 cut(s) 387
EcoT14I CCWWGG 3 cut(s) 69, 433, 832
ErhI CCWWGG 3 cut(s) 69, 433, 832
FaeI CATG 5 cut(s) 65, 92, 128, 437, 836
FaqI GGGAC 1 cut(s) 763
FatI CATG 5 cut(s) 61, 88, 124, 433, 832
FblI GTMKAC 2 cut(s) 744, 818
Fnu4HI GCNGC 4 cut(s) 173, 359, 537, 722
FokI GGATG 3 cut(s) 327, 671, 737
Fsp4HI GCNGC 4 cut(s) 173, 359, 537, 722
FspBI CTAG 2 cut(s) 270, 362
GluI GCNGC 4 cut(s) 173, 359, 537, 722
GsaI CCCAGC 1 cut(s) 457
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 177
Hin1I GRCGYC 1 cut(s) 741
Hin1II CATG 5 cut(s) 65, 92, 128, 437, 836
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 3 cut(s) 24, 128, 155
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 784
Hpy166II GTNNAC 4 cut(s) 192, 604, 745, 819
Hpy188I TCNGA 5 cut(s) 154, 187, 315, 461, 525
Hpy188III TCNNGA 5 cut(s) 18, 118, 209, 239, 531
Hpy8I GTNNAC 4 cut(s) 192, 604, 745, 819
Hpy99I CGWCG 2 cut(s) 315, 746
HpyAV CCTTC 2 cut(s) 406, 735
HpyCH4III ACNGT 2 cut(s) 378, 718
HpyCH4IV ACGT 4 cut(s) 31, 502, 711, 741
HpyCH4V TGCA 3 cut(s) 143, 192, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 358
HpyF3I CTNAG 2 cut(s) 458, 804
HpySE526I ACGT 4 cut(s) 31, 502, 711, 741
Hsp92I GRCGYC 1 cut(s) 741
Hsp92II CATG 5 cut(s) 65, 92, 128, 437, 836
Kzo9I GATC 5 cut(s) 77, 211, 448, 573, 836
LmnI GCTCC 1 cut(s) 260
Lsp1109I GCAGC 2 cut(s) 370, 708
LweI GCATC 4 cut(s) 62, 274, 759, 775
MaeI CTAG 2 cut(s) 270, 362
MaeII ACGT 4 cut(s) 31, 502, 711, 741
MaeIII GTNAC 2 cut(s) 226, 712
MalI GATC 5 cut(s) 79, 213, 450, 575, 838
MbiI CCGCTC 1 cut(s) 536
MboI GATC 5 cut(s) 77, 211, 448, 573, 836
MboII GAAGA 3 cut(s) 33, 316, 652
MflI RGATCY 2 cut(s) 573, 836
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 8 cut(s) 219, 233, 387, 406, 645, 700, 798, 810
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 2 cut(s) 293, 717
MnlI CCTC 3 cut(s) 131, 455, 556
MseI TTAA 4 cut(s) 167, 468, 489, 512
MslI CAYNNNNRTG 2 cut(s) 770, 853
MspCI CTTAAG 1 cut(s) 467
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 2 cut(s) 177, 178
MwoI GCNNNNNNNGC 1 cut(s) 358
NciI CCSGG 2 cut(s) 177, 178
NcoI CCATGG 2 cut(s) 433, 832
NdeII GATC 5 cut(s) 77, 211, 448, 573, 836
NheI GCTAGC 1 cut(s) 361
NlaIII CATG 5 cut(s) 65, 92, 128, 437, 836
NlaIV GGNNCC 2 cut(s) 256, 838
NmuCI GTSAC 2 cut(s) 226, 712
OliI CACNNNNGTG 1 cut(s) 770
PaeR7I CTCGAG 1 cut(s) 531
PfeI GAWTC 2 cut(s) 24, 155
Pfl23II CGTACG 1 cut(s) 28
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 4 cut(s) 174, 360, 538, 723
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Ppu21I YACGTR 1 cut(s) 712
PshAI GACNNNNGTC 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 453
PspLI CGTACG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 256, 838
PspPI GGNCC 1 cut(s) 665
PsuI RGATCY 2 cut(s) 573, 836
PsyI GACNNNGTC 1 cut(s) 204
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
RseI CAYNNNNRTG 2 cut(s) 770, 853
SalI GTCGAC 1 cut(s) 743
SaqAI TTAA 4 cut(s) 167, 468, 489, 512
SatI GCNGC 4 cut(s) 173, 359, 537, 722
Sau3AI GATC 5 cut(s) 77, 211, 448, 573, 836
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 177, 178
SduI GDGCHC 1 cut(s) 194
SfaNI GCATC 4 cut(s) 62, 274, 759, 775
SfcI CTRYAG 2 cut(s) 195, 577
Sfr274I CTCGAG 1 cut(s) 531
SinI GGWCC 1 cut(s) 665
SlaI CTCGAG 1 cut(s) 531
SmaI CCCGGG 1 cut(s) 178
SmiMI CAYNNNNRTG 2 cut(s) 770, 853
SmlI CTYRAG 3 cut(s) 467, 531, 683
SmoI CTYRAG 3 cut(s) 467, 531, 683
Sse9I AATT 8 cut(s) 219, 233, 387, 406, 645, 700, 798, 810
SsiI CCGC 2 cut(s) 173, 536
SspMI CTAG 2 cut(s) 270, 362
StyD4I CCNGG 2 cut(s) 175, 176
StyI CCWWGG 3 cut(s) 69, 433, 832
TaaI ACNGT 2 cut(s) 378, 718
TaiI ACGT 4 cut(s) 34, 505, 714, 744
TaqI TCGA 7 cut(s) 107, 131, 404, 424, 498, 532, 744
TasI AATT 8 cut(s) 219, 233, 387, 406, 645, 700, 798, 810
TauI GCSGC 2 cut(s) 175, 539
TfiI GAWTC 2 cut(s) 24, 155
Tru1I TTAA 4 cut(s) 167, 468, 489, 512
Tru9I TTAA 4 cut(s) 167, 468, 489, 512
TscAI CASTG 4 cut(s) 87, 231, 383, 774
TseFI GTSAC 2 cut(s) 226, 712
TseI GCWGC 2 cut(s) 358, 721
Tsp45I GTSAC 2 cut(s) 226, 712
TspDTI ATGAA 5 cut(s) 24, 77, 147, 258, 329
TspGWI ACGGA 1 cut(s) 637
TspMI CCCGGG 1 cut(s) 176
TspRI CASTG 4 cut(s) 87, 231, 383, 774
Tth111I GACNNNGTC 1 cut(s) 204
Vha464I CTTAAG 1 cut(s) 467
VneI GTGCAC 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 4 cut(s) 233, 387, 645, 700
XcmI CCANNNNNNNNNTGG 2 cut(s) 131, 351
XhoI CTCGAG 1 cut(s) 531
XmaI CCCGGG 1 cut(s) 176
XmiI GTMKAC 2 cut(s) 744, 818
XspI CTAG 2 cut(s) 270, 362
ZraI GACGTC 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.