Rroxscaffold_1G00003890

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002609
Physical Location & Seq
Forward (+)
5152008 .. 5154591
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003890.1

Sequence Viewer

Length: 858 bp
ATGTCATCTTTAAGCTGTGTCTTAGATAACCCACTAACCCAGTTCAGTATAATACAGCTGAATCTGTTTTGGAGTATGGCTTTGTTCATTTCCAGAAGAATCGTACGTTCATTTGGTTCTGGTTTAGCATCAGGCCATGTTTCCAAGGGTGGATCAGTGTTTTGTGCTTGTCGAATGCTTTCGACTTCAGTCTTGACCCATGAGTCGAGTGGAGGTGCATTTTGCTCTGATTCATTGTGCTTAAAGCCTCCCGGGGCTTCGGAGTGCACTACAGGCTCCACAACTTTGGCTAGTGATGTTTTTGATGCACCTATTACGATGGATATTGCTCGTCGGATATTTTTCTTCTTTCGTTGGCTTCATCCAGTTGTGCTGTGGCAGCTAGCAAAAAGGCAACAGCCATTGAATTGGAAGGAGAGTGGTCGAGCCAGCCATGGAACATTGAGTGATCCCCGGCTCAGAGGTCTTAAGCCACGAAGTCATACTATTAAGAATGTCGAACGTCTGGGATTAAAGATTGCTCTGACATCTCGAGCGGCACAGGGAAAGCTTCTGGTTTTTGAGGATTTTGGGATCTACAGAAATAAACCCAAGAACATTGTGAACTACGTCAAGCAAAGACGGAAAAAGGAGAAAAAGAAGAAAATTCTGCTGGTGGATGGTGGTCCAATAAGTGAAAAGCTCAAGTTGGCTACTAAAAATTTACATTACGTGACTGTGCTGCCTTCAAGTGAGTTGGACGTCGACAGCATCCTTTACTATGACACACTGGTGATGTCCCGTGATGCTGTAAATAAAATTGCTGAGCAAATTATGTATACTCCAATCAACCATGGATCCAGCACGACATACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

285

Amino Acids

31.86

Weight (kDa)

9.85

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 132 - 269 1.7e-19 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 202
AatII GACGTC 1 cut(s) 744
AccBSI CCGCTC 1 cut(s) 536
AccI GTMKAC 2 cut(s) 744, 818
AciI CCGC 1 cut(s) 536
AclWI GGATC 5 cut(s) 160, 443, 581, 831, 844
AcsI RAATTY 2 cut(s) 645, 700
AcuI CTGAAG 1 cut(s) 171
AcyI GRCGYC 1 cut(s) 741
AfaI GTAC 1 cut(s) 105
AflII CTTAAG 1 cut(s) 467
AgsI TTSAA 2 cut(s) 406, 729
AleI CACNNNNGTG 1 cut(s) 770
AluBI AGCT 5 cut(s) 15, 58, 382, 550, 682
AluI AGCT 5 cut(s) 15, 58, 382, 550, 682
Alw21I GWGCWC 1 cut(s) 269
Alw44I GTGCAC 1 cut(s) 265
AlwI GGATC 5 cut(s) 160, 443, 581, 831, 844
Ama87I CYCGRG 2 cut(s) 251, 531
AoxI GGCC 1 cut(s) 133
ApaLI GTGCAC 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 379, 721
ApoI RAATTY 2 cut(s) 645, 700
Asp700I GAANNNNTTC 1 cut(s) 178
AspS9I GGNCC 1 cut(s) 665
AsuC2I CCSGG 3 cut(s) 252, 253, 454
AsuHPI GGTGA 1 cut(s) 784
AsuNHI GCTAGC 1 cut(s) 382
AvaI CYCGRG 2 cut(s) 251, 531
AvaII GGWCC 1 cut(s) 665
BaeGI GKGCMC 1 cut(s) 269
BamHI GGATCC 1 cut(s) 836
Bbv12I GWGCWC 1 cut(s) 269
BbvI GCAGC 2 cut(s) 391, 708
BccI CCATC 2 cut(s) 313, 653
BcnI CCSGG 3 cut(s) 252, 253, 454
BfaI CTAG 2 cut(s) 291, 383
BfmI CTRYAG 2 cut(s) 270, 577
BfrI CTTAAG 1 cut(s) 467
BisI GCNGC 3 cut(s) 380, 537, 722
BlpI GCTNAGC 1 cut(s) 804
BlsI GCNGC 3 cut(s) 381, 538, 723
Bme1390I CCNGG 3 cut(s) 252, 253, 454
Bme18I GGWCC 1 cut(s) 665
BmeT110I CYCGRG 2 cut(s) 251, 531
BmgT120I GGNCC 1 cut(s) 665
BmiI GGNNCC 2 cut(s) 277, 838
BmrFI CCNGG 3 cut(s) 252, 253, 454
BmrI ACTGGG 1 cut(s) 34
BmsI GCATC 4 cut(s) 137, 295, 759, 775
BmtI GCTAGC 1 cut(s) 386
BmuI ACTGGG 1 cut(s) 34
BoxI GACNNNNGTC 1 cut(s) 188
Bpu1102I GCTNAGC 1 cut(s) 804
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 3 cut(s) 252, 253, 454
BsaAI YACGTR 1 cut(s) 712
BsaHI GRCGYC 1 cut(s) 741
BsaJI CCNNGG 6 cut(s) 144, 251, 252, 433, 452, 832
Bse1I ACTGG 4 cut(s) 40, 365, 774, 857
BseDI CCNNGG 6 cut(s) 144, 251, 252, 433, 452, 832
BseGI GGATG 3 cut(s) 361, 664, 750
BseMII CTCAG 2 cut(s) 472, 795
BseNI ACTGG 4 cut(s) 40, 365, 774, 857
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 2 cut(s) 391, 708
BshFI GGCC 1 cut(s) 135
BsiHKAI GWGCWC 1 cut(s) 269
BsiHKCI CYCGRG 2 cut(s) 251, 531
BsiSI CCGG 2 cut(s) 252, 454
BsiWI CGTACG 1 cut(s) 103
BslFI GGGAC 1 cut(s) 763
BsmFI GGGAC 1 cut(s) 763
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 1 cut(s) 135
BsoBI CYCGRG 2 cut(s) 251, 531
Bsp1286I GDGCHC 1 cut(s) 269
Bsp143I GATC 4 cut(s) 152, 448, 573, 836
Bsp1720I GCTNAGC 1 cut(s) 804
Bsp19I CCATGG 2 cut(s) 433, 832
BspACI CCGC 1 cut(s) 536
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 2 cut(s) 471, 796
BspLI GGNNCC 2 cut(s) 277, 838
BspOI GCTAGC 1 cut(s) 386
BspPI GGATC 5 cut(s) 160, 443, 581, 831, 844
BspTI CTTAAG 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 536
BsrI ACTGG 4 cut(s) 40, 365, 774, 857
BssECI CCNNGG 6 cut(s) 144, 251, 252, 433, 452, 832
BssMI GATC 4 cut(s) 152, 448, 573, 836
BssNAI GTATAC 1 cut(s) 819
BssNI GRCGYC 1 cut(s) 741
BssT1I CCWWGG 3 cut(s) 144, 433, 832
Bst1107I GTATAC 1 cut(s) 819
Bst4CI ACNGT 1 cut(s) 718
BstACI GRCGYC 1 cut(s) 741
BstAFI CTTAAG 1 cut(s) 467
BstBAI YACGTR 1 cut(s) 712
BstC8I GCNNGC 2 cut(s) 384, 430
BstDEI CTNAG 3 cut(s) 22, 458, 804
BstDSI CCRYGG 2 cut(s) 433, 832
BstF5I GGATG 3 cut(s) 361, 664, 750
BstKTI GATC 4 cut(s) 155, 451, 576, 839
BstMBI GATC 4 cut(s) 152, 448, 573, 836
BstMWI GCNNNNNNNGC 2 cut(s) 273, 379
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 3 cut(s) 250, 251, 452
BstSFI CTRYAG 2 cut(s) 270, 577
BstSLI GKGCMC 1 cut(s) 269
BstV1I GCAGC 2 cut(s) 391, 708
BstX2I RGATCY 2 cut(s) 573, 836
BstXI CCANNNNNNTGG 2 cut(s) 286, 408
BstYI RGATCY 2 cut(s) 573, 836
BstZ17I GTATAC 1 cut(s) 819
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 2 cut(s) 433, 832
BtsCI GGATG 3 cut(s) 361, 664, 750
BtsIMutI CAGTG 2 cut(s) 162, 767
Cac8I GCNNGC 2 cut(s) 384, 430
Cfr13I GGNCC 1 cut(s) 665
Cfr9I CCCGGG 1 cut(s) 251
Csp6I GTAC 1 cut(s) 104
CviAII CATG 4 cut(s) 137, 200, 434, 833
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 3 cut(s) 22, 458, 804
DpnI GATC 4 cut(s) 154, 450, 575, 838
DpnII GATC 4 cut(s) 152, 448, 573, 836
DrdI GACNNNNNNGTC 1 cut(s) 202
DseDI GACNNNNNNGTC 1 cut(s) 202
Eco130I CCWWGG 3 cut(s) 144, 433, 832
Eco47I GGWCC 1 cut(s) 665
Eco57I CTGAAG 1 cut(s) 171
Eco88I CYCGRG 2 cut(s) 251, 531
EcoT14I CCWWGG 3 cut(s) 144, 433, 832
ErhI CCWWGG 3 cut(s) 144, 433, 832
FaeI CATG 4 cut(s) 140, 203, 437, 836
FaqI GGGAC 1 cut(s) 763
FatI CATG 4 cut(s) 136, 199, 433, 832
FblI GTMKAC 2 cut(s) 744, 818
Fnu4HI GCNGC 3 cut(s) 380, 537, 722
FokI GGATG 3 cut(s) 348, 671, 737
Fsp4HI GCNGC 3 cut(s) 380, 537, 722
FspBI CTAG 2 cut(s) 291, 383
GluI GCNGC 3 cut(s) 380, 537, 722
HaeIII GGCC 1 cut(s) 135
HapII CCGG 2 cut(s) 252, 454
Hin1I GRCGYC 1 cut(s) 741
Hin1II CATG 4 cut(s) 140, 203, 437, 836
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 4 cut(s) 61, 99, 203, 230
HpaII CCGG 2 cut(s) 252, 454
HphI GGTGA 1 cut(s) 784
Hpy166II GTNNAC 4 cut(s) 267, 604, 745, 819
Hpy188I TCNGA 5 cut(s) 229, 262, 336, 461, 525
Hpy188III TCNNGA 3 cut(s) 93, 193, 531
Hpy8I GTNNAC 4 cut(s) 267, 604, 745, 819
Hpy99I CGWCG 2 cut(s) 336, 746
HpyAV CCTTC 2 cut(s) 406, 735
HpyCH4III ACNGT 1 cut(s) 718
HpyCH4IV ACGT 5 cut(s) 106, 502, 609, 711, 741
HpyCH4V TGCA 3 cut(s) 218, 267, 308
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 379
HpyF3I CTNAG 3 cut(s) 22, 458, 804
HpySE526I ACGT 5 cut(s) 106, 502, 609, 711, 741
Hsp92I GRCGYC 1 cut(s) 741
Hsp92II CATG 4 cut(s) 140, 203, 437, 836
Kzo9I GATC 4 cut(s) 152, 448, 573, 836
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 2 cut(s) 391, 708
LweI GCATC 4 cut(s) 137, 295, 759, 775
MaeI CTAG 2 cut(s) 291, 383
MaeII ACGT 5 cut(s) 106, 502, 609, 711, 741
MaeIII GTNAC 1 cut(s) 712
MalI GATC 4 cut(s) 154, 450, 575, 838
MbiI CCGCTC 1 cut(s) 536
MboI GATC 4 cut(s) 152, 448, 573, 836
MboII GAAGA 3 cut(s) 108, 337, 652
MflI RGATCY 2 cut(s) 573, 836
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 5 cut(s) 406, 645, 700, 798, 810
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 2 cut(s) 314, 717
MnlI CCTC 4 cut(s) 206, 258, 455, 556
MroXI GAANNNNTTC 1 cut(s) 178
MseI TTAA 5 cut(s) 11, 242, 468, 489, 512
MslI CAYNNNNRTG 2 cut(s) 770, 853
MspA1I CMGCKG 1 cut(s) 58
MspCI CTTAAG 1 cut(s) 467
MspI CCGG 2 cut(s) 252, 454
MspR9I CCNGG 3 cut(s) 252, 253, 454
Mva1269I GAATGC 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 273, 379
NciI CCSGG 3 cut(s) 252, 253, 454
NcoI CCATGG 2 cut(s) 433, 832
NdeII GATC 4 cut(s) 152, 448, 573, 836
NheI GCTAGC 1 cut(s) 382
NlaIII CATG 4 cut(s) 140, 203, 437, 836
NlaIV GGNNCC 2 cut(s) 277, 838
NmuCI GTSAC 1 cut(s) 712
OliI CACNNNNGTG 1 cut(s) 770
PaeR7I CTCGAG 1 cut(s) 531
PctI GAATGC 1 cut(s) 180
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 3 cut(s) 61, 99, 230
Pfl23II CGTACG 1 cut(s) 103
PkrI GCNGC 3 cut(s) 381, 538, 723
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Ppu21I YACGTR 1 cut(s) 712
PshAI GACNNNNGTC 1 cut(s) 188
PspLI CGTACG 1 cut(s) 103
PspN4I GGNNCC 2 cut(s) 277, 838
PspPI GGNCC 1 cut(s) 665
PsuI RGATCY 2 cut(s) 573, 836
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
RseI CAYNNNNRTG 2 cut(s) 770, 853
SalI GTCGAC 1 cut(s) 743
SaqAI TTAA 5 cut(s) 11, 242, 468, 489, 512
SatI GCNGC 3 cut(s) 380, 537, 722
Sau3AI GATC 4 cut(s) 152, 448, 573, 836
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 3 cut(s) 252, 253, 454
SduI GDGCHC 1 cut(s) 269
SfaNI GCATC 4 cut(s) 137, 295, 759, 775
SfcI CTRYAG 2 cut(s) 270, 577
Sfr274I CTCGAG 1 cut(s) 531
SinI GGWCC 1 cut(s) 665
SlaI CTCGAG 1 cut(s) 531
SmaI CCCGGG 1 cut(s) 253
SmiMI CAYNNNNRTG 2 cut(s) 770, 853
SmlI CTYRAG 3 cut(s) 467, 531, 683
SmoI CTYRAG 3 cut(s) 467, 531, 683
Sse9I AATT 5 cut(s) 406, 645, 700, 798, 810
SsiI CCGC 1 cut(s) 536
SspMI CTAG 2 cut(s) 291, 383
StyD4I CCNGG 3 cut(s) 250, 251, 452
StyI CCWWGG 3 cut(s) 144, 433, 832
TaaI ACNGT 1 cut(s) 718
TaiI ACGT 5 cut(s) 109, 505, 612, 714, 744
TaqI TCGA 7 cut(s) 172, 182, 206, 424, 498, 532, 744
TasI AATT 5 cut(s) 406, 645, 700, 798, 810
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 3 cut(s) 61, 99, 230
Tru1I TTAA 5 cut(s) 11, 242, 468, 489, 512
Tru9I TTAA 5 cut(s) 11, 242, 468, 489, 512
TscAI CASTG 2 cut(s) 162, 774
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 2 cut(s) 379, 721
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 4 cut(s) 76, 99, 222, 350
TspGWI ACGGA 1 cut(s) 637
TspMI CCCGGG 1 cut(s) 251
TspRI CASTG 2 cut(s) 162, 774
Vha464I CTTAAG 1 cut(s) 467
VneI GTGCAC 1 cut(s) 265
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 2 cut(s) 645, 700
XcmI CCANNNNNNNNNTGG 2 cut(s) 206, 372
XhoI CTCGAG 1 cut(s) 531
XmaI CCCGGG 1 cut(s) 251
XmiI GTMKAC 2 cut(s) 744, 818
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 2 cut(s) 291, 383
ZraI GACGTC 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.