Rh5BG019800

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002609
Physical Location & Seq
Forward (+)
1490967 .. 1494766
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG019800.1

Sequence Viewer

Length: 1053 bp
ATGTGTTTGCTTCCGGGTCTTCTTTCATTTTCGGTACCCTGGCTCCGGCTAGCTCTCACTCTCTCCTCTCTCACATTGCCGCCATCGGCAGAGCACTCTGTAGCGGAAGGATCGACGGTTGTCAATCTCCGACGCATTTCGACGGCCAGATTTCCGGCGTCTACGGCTTCGAAGAGAGGATTAGAAGGCAGCTATGACTCTCCGAGTATATTATATCCATCGTTTCACGCTTCTCATGGGGTTATCTTTTCCTCTGAGGAATCTTCGGCTGTTCCCAAGGGTTGGTCATCTTCTTATGCTTATCGAAGGCTTTCAACTCTGACTCATGAATCAAGTGGAGGTGCAATTCCCTCTGATCTATTATCCAAAAAGCCTGTGGTAGCGTCAGAGCATAAAATAGGAAATTATCAAGACCTTGTAATTCCAGTGACAAATTTTCATAATGAAGACAAAGGCTTGATGGTCTTGCCTGGTGATGTTTTTGATGTTCCTATTAGAAAGGATATTGTTCACCGGGTTGTACTATGGCAGCTAGCAAAAAGGCAACAGGGAACACATTCAACAAAAACTATCAGTGAGGTCAGCGGGACTGGGAGAAAGCCATGGAATCAGAAGGGTACTGGTCGAGCGAGGCATGGGACTTTGCGTGGTCCTCAGTTCCGAGGAGGTGCGACAATGCATGGTCCTAAGCCACGAAGTCATGCTATCAAGCTGAATAAGAAGGTTCGGCGTCTTGGGTTAAAAGTTGCTCTGACAGCTCGAGCAGCAGAGGGAAAGCTTCTTGTTTTCGAGGACTTGGAAATCCCCACACATAAAACCAAGAATATTGTGAACTATGTCAAGCAAATGGAGAACACCAAGAAAGTTCTAGTGGTGGATGGCGGCCCCATAAGTGATAAACTCAAGCTGGCAACTCAAAACATACATTATGTGAACGTGCTGCCCTCAATTGGTTTGAATGTCTACAGCATCCTGCAGCACGACACACTGGTGATGTCTCGCGATGCTATAGACAAAATTGTTGAACGGATGCACACTCCAATTAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

350

Amino Acids

38.46

Weight (kDa)

10.07

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 156 - 343 2.1e-70 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 34
AccB1I GGYRCC 1 cut(s) 34
AccB7I CCANNNNNTGG 1 cut(s) 282
AccI GTMKAC 2 cut(s) 161, 963
AccII CGCG 1 cut(s) 1002
AciI CCGC 4 cut(s) 80, 104, 585, 882
AclWI GGATC 1 cut(s) 118
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 1 cut(s) 433
AcyI GRCGYC 2 cut(s) 158, 730
AfaI GTAC 3 cut(s) 36, 522, 619
AfiI CCNNNNNNNGG 3 cut(s) 45, 282, 950
AgsI TTSAA 4 cut(s) 315, 561, 958, 1025
AjnI CCWGG 2 cut(s) 38, 469
AleI CACNNNNGTG 1 cut(s) 989
AluBI AGCT 7 cut(s) 53, 192, 532, 712, 758, 778, 907
AluI AGCT 7 cut(s) 53, 192, 532, 712, 758, 778, 907
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 1002
AlwI GGATC 1 cut(s) 118
Ama87I CYCGRG 1 cut(s) 759
AoxI GGCC 2 cut(s) 144, 883
ApeKI GCWGC 5 cut(s) 189, 529, 764, 940, 976
ApoI RAATTY 1 cut(s) 433
Asp700I GAANNNNTTC 2 cut(s) 310, 556
Asp718I GGTACC 1 cut(s) 34
AspS9I GGNCC 3 cut(s) 650, 683, 884
AsuC2I CCSGG 2 cut(s) 15, 515
AsuHPI GGTGA 3 cut(s) 485, 503, 1003
AsuII TTCGAA 1 cut(s) 170
AsuNHI GCTAGC 2 cut(s) 49, 532
AvaI CYCGRG 1 cut(s) 759
AvaII GGWCC 2 cut(s) 650, 683
BanI GGYRCC 1 cut(s) 34
BbsI GAAGAC 2 cut(s) 11, 453
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 5 cut(s) 201, 541, 776, 927, 988
BccI CCATC 4 cut(s) 91, 226, 454, 872
BceAI ACGGC 2 cut(s) 159, 180
BciT130I CCWGG 2 cut(s) 40, 471
BcnI CCSGG 2 cut(s) 15, 515
BcoDI GTCTC 1 cut(s) 1002
BfaI CTAG 3 cut(s) 50, 533, 869
BfmI CTRYAG 4 cut(s) 99, 964, 974, 1008
BisI GCNGC 7 cut(s) 80, 190, 530, 765, 883, 941, 977
BlsI GCNGC 7 cut(s) 81, 191, 531, 766, 884, 942, 978
Bme1390I CCNGG 4 cut(s) 15, 40, 471, 515
Bme18I GGWCC 2 cut(s) 650, 683
BmeT110I CYCGRG 1 cut(s) 759
BmgT120I GGNCC 3 cut(s) 650, 683, 884
BmiI GGNNCC 3 cut(s) 36, 44, 886
BmrFI CCNGG 4 cut(s) 15, 40, 471, 515
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 3 cut(s) 978, 994, 1020
BmtI GCTAGC 2 cut(s) 53, 536
BmuI ACTGGG 1 cut(s) 600
BoxI GACNNNNGTC 1 cut(s) 119
BpiI GAAGAC 2 cut(s) 11, 453
Bpu10I CCTNAGC 1 cut(s) 687
Bpu14I TTCGAA 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 887
BpuMI CCSGG 2 cut(s) 15, 515
BsaHI GRCGYC 2 cut(s) 158, 730
BsaJI CCNNGG 4 cut(s) 38, 276, 602, 661
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 282, 950
Bse1I ACTGG 4 cut(s) 425, 595, 625, 993
Bse3DI GCAATG 1 cut(s) 74
BseBI CCWGG 2 cut(s) 40, 471
BseDI CCNNGG 4 cut(s) 38, 276, 602, 661
BseGI GGATG 3 cut(s) 883, 969, 1035
BseLI CCNNNNNNNGG 3 cut(s) 45, 282, 950
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 2 cut(s) 246, 668
BseNI ACTGG 4 cut(s) 425, 595, 625, 993
BseRI GAGGAG 2 cut(s) 55, 678
BseXI GCAGC 5 cut(s) 201, 541, 776, 927, 988
Bsh1236I CGCG 1 cut(s) 1002
BshFI GGCC 2 cut(s) 146, 885
BshNI GGYRCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 759
BsiSI CCGG 4 cut(s) 14, 46, 155, 514
BslFI GGGAC 2 cut(s) 601, 652
BslI CCNNNNNNNGG 3 cut(s) 45, 282, 950
BsmAI GTCTC 1 cut(s) 1002
BsmFI GGGAC 2 cut(s) 601, 652
BsnI GGCC 2 cut(s) 146, 885
BsoBI CYCGRG 1 cut(s) 759
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 110, 355
Bsp19I CCATGG 1 cut(s) 602
Bsp68I TCGCGA 1 cut(s) 1002
BspACI CCGC 4 cut(s) 80, 104, 585, 882
BspANI GGCC 2 cut(s) 146, 885
BspCNI CTCAG 2 cut(s) 247, 667
BspFNI CGCG 1 cut(s) 1002
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 3 cut(s) 36, 44, 886
BspMAI CTGCAG 1 cut(s) 978
BspOI GCTAGC 2 cut(s) 53, 536
BspPI GGATC 1 cut(s) 118
BspT104I TTCGAA 1 cut(s) 170
BspT107I GGYRCC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 74
BsrI ACTGG 4 cut(s) 425, 595, 625, 993
BssECI CCNNGG 4 cut(s) 38, 276, 602, 661
BssMI GATC 2 cut(s) 110, 355
BssNI GRCGYC 2 cut(s) 158, 730
BssT1I CCWWGG 2 cut(s) 276, 602
Bst2UI CCWGG 2 cut(s) 40, 471
Bst4CI ACNGT 2 cut(s) 118, 1049
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 2 cut(s) 158, 730
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 51, 534, 909
BstDEI CTNAG 3 cut(s) 255, 654, 687
BstDSI CCRYGG 1 cut(s) 602
BstF5I GGATG 3 cut(s) 883, 969, 1035
BstFNI CGCG 1 cut(s) 1002
BstKTI GATC 2 cut(s) 113, 358
BstMAI GTCTC 1 cut(s) 1002
BstMBI GATC 2 cut(s) 110, 355
BstMWI GCNNNNNNNGC 3 cut(s) 164, 755, 764
BstNI CCWGG 2 cut(s) 40, 471
BstPAI GACNNNNGTC 1 cut(s) 119
BstSCI CCNGG 4 cut(s) 13, 38, 469, 513
BstSFI CTRYAG 4 cut(s) 99, 964, 974, 1008
BstUI CGCG 1 cut(s) 1002
BstV1I GCAGC 5 cut(s) 201, 541, 776, 927, 988
BstV2I GAAGAC 2 cut(s) 11, 453
BsuRI GGCC 2 cut(s) 146, 885
BtgI CCRYGG 1 cut(s) 602
BtgZI GCGATG 1 cut(s) 1017
BtsCI GGATG 3 cut(s) 883, 969, 1035
BtsIMutI CAGTG 3 cut(s) 432, 580, 986
BtuMI TCGCGA 1 cut(s) 1002
Cac8I GCNNGC 3 cut(s) 51, 534, 909
CciI TCATGA 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 650, 683, 884
CseI GACGC 4 cut(s) 141, 147, 372, 719
Csp6I GTAC 3 cut(s) 35, 521, 618
CviAII CATG 6 cut(s) 236, 326, 603, 635, 680, 701
CviQI GTAC 3 cut(s) 35, 521, 618
DdeI CTNAG 3 cut(s) 255, 654, 687
DpnI GATC 2 cut(s) 112, 357
DpnII GATC 2 cut(s) 110, 355
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Eco130I CCWWGG 2 cut(s) 276, 602
Eco47I GGWCC 2 cut(s) 650, 683
Eco88I CYCGRG 1 cut(s) 759
EcoRII CCWGG 2 cut(s) 38, 469
EcoT14I CCWWGG 2 cut(s) 276, 602
EcoT22I ATGCAT 1 cut(s) 681
ErhI CCWWGG 2 cut(s) 276, 602
FaeI CATG 6 cut(s) 239, 329, 606, 638, 683, 704
FaqI GGGAC 2 cut(s) 601, 652
FatI CATG 6 cut(s) 235, 325, 602, 634, 679, 700
FauI CCCGC 1 cut(s) 578
FblI GTMKAC 2 cut(s) 161, 963
Fnu4HI GCNGC 7 cut(s) 80, 190, 530, 765, 883, 941, 977
FokI GGATG 3 cut(s) 890, 956, 1042
Fsp4HI GCNGC 7 cut(s) 80, 190, 530, 765, 883, 941, 977
FspBI CTAG 3 cut(s) 50, 533, 869
GluI GCNGC 7 cut(s) 80, 190, 530, 765, 883, 941, 977
HaeIII GGCC 2 cut(s) 146, 885
HapII CCGG 4 cut(s) 14, 46, 155, 514
HgaI GACGC 4 cut(s) 141, 147, 372, 719
Hin1I GRCGYC 2 cut(s) 158, 730
Hin1II CATG 6 cut(s) 239, 329, 606, 638, 683, 704
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 5 cut(s) 197, 260, 322, 329, 607
HpaII CCGG 4 cut(s) 14, 46, 155, 514
HphI GGTGA 3 cut(s) 485, 503, 1003
Hpy166II GTNNAC 5 cut(s) 162, 511, 832, 934, 964
Hpy188I TCNGA 9 cut(s) 131, 204, 256, 321, 355, 388, 612, 662, 753
Hpy188III TCNNGA 3 cut(s) 326, 410, 1001
Hpy8I GTNNAC 5 cut(s) 162, 511, 832, 934, 964
Hpy99I CGWCG 3 cut(s) 118, 135, 145
HpyAV CCTTC 5 cut(s) 101, 179, 300, 607, 715
HpyCH4III ACNGT 2 cut(s) 118, 1049
HpyCH4IV ACGT 1 cut(s) 936
HpyCH4V TGCA 4 cut(s) 344, 679, 976, 1033
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 755, 764
HpyF3I CTNAG 3 cut(s) 255, 654, 687
HpySE526I ACGT 1 cut(s) 936
Hsp92I GRCGYC 2 cut(s) 158, 730
Hsp92II CATG 6 cut(s) 239, 329, 606, 638, 683, 704
KpnI GGTACC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 110, 355
LmnI GCTCC 1 cut(s) 48
Lsp1109I GCAGC 5 cut(s) 201, 541, 776, 927, 988
LweI GCATC 3 cut(s) 978, 994, 1020
MaeI CTAG 3 cut(s) 50, 533, 869
MaeII ACGT 1 cut(s) 936
MaeIII GTNAC 1 cut(s) 427
MalI GATC 2 cut(s) 112, 357
MboI GATC 2 cut(s) 110, 355
MboII GAAGA 5 cut(s) 11, 184, 255, 282, 458
MfeI CAATTG 1 cut(s) 948
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 7 cut(s) 345, 403, 420, 433, 948, 1017, 1041
MlyI GAGTC 2 cut(s) 191, 316
MmeI TCCRAC 1 cut(s) 154
Mph1103I ATGCAT 1 cut(s) 681
MroXI GAANNNNTTC 2 cut(s) 310, 556
MseI TTAA 2 cut(s) 740, 1044
MslI CAYNNNNRTG 1 cut(s) 989
MspA1I CMGCKG 1 cut(s) 585
MspI CCGG 4 cut(s) 14, 46, 155, 514
MspR9I CCNGG 4 cut(s) 15, 40, 471, 515
MunI CAATTG 1 cut(s) 948
MvaI CCWGG 2 cut(s) 40, 471
MvnI CGCG 1 cut(s) 1002
MwoI GCNNNNNNNGC 3 cut(s) 164, 755, 764
NciI CCSGG 2 cut(s) 15, 515
NcoI CCATGG 1 cut(s) 602
NdeII GATC 2 cut(s) 110, 355
NheI GCTAGC 2 cut(s) 49, 532
NlaIII CATG 6 cut(s) 239, 329, 606, 638, 683, 704
NlaIV GGNNCC 3 cut(s) 36, 44, 886
NmuCI GTSAC 1 cut(s) 427
NruI TCGCGA 1 cut(s) 1002
NsiI ATGCAT 1 cut(s) 681
NspV TTCGAA 1 cut(s) 170
OliI CACNNNNGTG 1 cut(s) 989
PaeR7I CTCGAG 1 cut(s) 759
PagI TCATGA 1 cut(s) 325
PdmI GAANNNNTTC 2 cut(s) 310, 556
PfeI GAWTC 3 cut(s) 260, 329, 607
PflMI CCANNNNNTGG 1 cut(s) 282
PkrI GCNGC 7 cut(s) 81, 191, 531, 766, 884, 942, 978
PleI GAGTC 2 cut(s) 191, 316
PpsI GAGTC 2 cut(s) 191, 316
PshAI GACNNNNGTC 1 cut(s) 119
Psp6I CCWGG 2 cut(s) 38, 469
PspGI CCWGG 2 cut(s) 38, 469
PspN4I GGNNCC 3 cut(s) 36, 44, 886
PspPI GGNCC 3 cut(s) 650, 683, 884
PspXI VCTCGAGB 1 cut(s) 759
PstI CTGCAG 1 cut(s) 978
RruI TCGCGA 1 cut(s) 1002
RsaI GTAC 3 cut(s) 36, 522, 619
RsaNI GTAC 3 cut(s) 35, 521, 618
RseI CAYNNNNRTG 1 cut(s) 989
SaqAI TTAA 2 cut(s) 740, 1044
SatI GCNGC 7 cut(s) 80, 190, 530, 765, 883, 941, 977
Sau3AI GATC 2 cut(s) 110, 355
Sau96I GGNCC 3 cut(s) 650, 683, 884
SchI GAGTC 2 cut(s) 191, 316
ScrFI CCNGG 4 cut(s) 15, 40, 471, 515
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 3 cut(s) 978, 994, 1020
SfcI CTRYAG 4 cut(s) 99, 964, 974, 1008
Sfr274I CTCGAG 1 cut(s) 759
SfuI TTCGAA 1 cut(s) 170
SinI GGWCC 2 cut(s) 650, 683
SlaI CTCGAG 1 cut(s) 759
SmiMI CAYNNNNRTG 1 cut(s) 989
SmlI CTYRAG 2 cut(s) 759, 902
SmoI CTYRAG 2 cut(s) 759, 902
Sse9I AATT 7 cut(s) 345, 403, 420, 433, 948, 1017, 1041
SsiI CCGC 4 cut(s) 80, 104, 585, 882
SspI AATATT 1 cut(s) 826
SspMI CTAG 3 cut(s) 50, 533, 869
StyD4I CCNGG 4 cut(s) 13, 38, 469, 513
StyI CCWWGG 2 cut(s) 276, 602
TaaI ACNGT 2 cut(s) 118, 1049
TaiI ACGT 1 cut(s) 939
TaqI TCGA 7 cut(s) 113, 140, 170, 304, 625, 760, 789
TasI AATT 7 cut(s) 345, 403, 420, 433, 948, 1017, 1041
TatI WGTACW 1 cut(s) 520
TauI GCSGC 2 cut(s) 82, 885
TfiI GAWTC 3 cut(s) 260, 329, 607
Tru1I TTAA 2 cut(s) 740, 1044
Tru9I TTAA 2 cut(s) 740, 1044
TscAI CASTG 3 cut(s) 432, 580, 993
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 5 cut(s) 189, 529, 764, 940, 976
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 4 cut(s) 15, 342, 428, 459
TspGWI ACGGA 1 cut(s) 1042
TspRI CASTG 3 cut(s) 432, 580, 993
Van91I CCANNNNNTGG 1 cut(s) 282
VpaK11BI GGWCC 2 cut(s) 650, 683
XapI RAATTY 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 759
XmiI GTMKAC 2 cut(s) 161, 963
XmnI GAANNNNTTC 2 cut(s) 310, 556
XspI CTAG 3 cut(s) 50, 533, 869
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.